[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 831.64 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk21-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk21-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk21.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.0 = CAAGAAAGTTGGGACA-1

using 42297 reads

====================================================================================

graph has 21134 edges initially, 676 edges after simplification

total ucounts = 1107
nonsolo ucounts = 955[2^60, 3^47, 4^38, 5^36, 6^28, 7^26, 8^34, 9^22, 10^23, 11^16, 12^17, 13^14, 14^24, 15^11, 16^17, 17^16, 18^13, 19^11, 20^16, 21^7, 22^10, 23^12, 24^17, 25^3, 26^14, 27^14, 28^12, 29^13, 30^5, 31^12, 32^6, 33^3, 34^7, 35^11, 36^8, 37^4, 38^8, 39^7, 40^7, 41^11, 42^9, 43^4, 44^6, 45^2, 46^7, 47^4, 48^4, 49^8, 50^6, 51^4, 52^3, 53^9, 54^9, 55^3, 56^4, 57^3, 58^5, 59^4, 60^7, 61^5, 62^5, 63^7, 64^4, 65^10, 66^3, 67^4, 68^2, 69^5, 70^6, 71^4, 72^7, 73^3, 74^4, 75^3, 76^5, 77^5, 78, 79^7, 80, 81^3, 82^2, 83^3, 84^6, 85^5, 86^3, 87^2, 88, 89^5, 90^3, 91^3, 92^3, 93^2, 94^2, 95^2, 96, 97^3, 98^2, 100, 101, 102^2, 103^2, 104, 105^2, 108^2, 109^2, 110^3, 112^3, 113, 115^2, 116^2, 119^2, 121^2, 122^2, 123, 126, 127, 128, 129^2, 132, 134, 136, 137, 139, 141, 142, 143^2, 145, 148, 150, 157, 169, 170, 10123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.1 = CAAGAAAGTTGTCTTT-1

using 63 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3^2, 4, 9, 19, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.2 = CAAGAAATCAAAGTAG-1

using 397 reads

====================================================================================

graph has 284 edges initially, 8 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^5, 4, 6, 11, 19, 20^2, 31, 36, 70, 77, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.6 = CAAGAAATCACTTCAT-1

using 33 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.8 = CAAGAAATCCAAACAC-1

using 203 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 198]
surviving nonsolo ucounts = 1[198]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.9 = CAAGAAATCCAGAAGG-1

using 57 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.11 = CAAGAAATCCCTCTTT-1

using 113 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.15 = CAAGAAATCCTTGACC-1

using 72 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2^2, 3^2, 4^2, 5, 10, 12, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.21 = CAAGAAATCTGCGACG-1

using 20 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.22 = CAAGAAATCTGGCGTG-1

using 214 reads

====================================================================================

graph has 220 edges initially, 6 edges after simplification

total ucounts = 19
nonsolo ucounts = 15[2^2, 5, 6^2, 7, 9^2, 10, 14, 15, 17, 18, 24, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.24 = CAAGAAATCTTGAGGT-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.25 = CAAGAAATCTTGTATC-1

using 75 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.27 = CAAGAAATCTTTACGT-1

using 25 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.31 = CAAGATCAGACTTGAA-1

using 308 reads

====================================================================================

graph has 318 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 17[6^3, 9^2, 11^2, 13, 16, 19, 20, 21, 24, 27, 28, 31, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.32 = CAAGATCAGAGGTTGC-1

using 233 reads

====================================================================================

graph has 132 edges initially, 8 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[4, 7^2, 11, 12^2, 14^3, 17, 20, 31^2, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.36 = CAAGATCAGATGTGGC-1

using 481 reads

====================================================================================

graph has 294 edges initially, 10 edges after simplification

total ucounts = 25
nonsolo ucounts = 21[2, 3, 5, 6, 10, 12, 13, 14^3, 21^2, 24, 25, 27, 28, 30, 34, 40, 45, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.37 = CAAGATCAGCAAATCA-1

using 401 reads

====================================================================================

graph has 290 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^5, 3^3, 6, 8, 13, 15^2, 17, 41, 44, 48, 50, 121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.41 = CAAGATCAGCCTTGAT-1

using 204 reads

====================================================================================

graph has 151 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[2^4, 5, 6^2, 8, 10^2, 13, 14^2, 20, 27, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.43 = CAAGATCAGCGCCTTG-1

using 60 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.45 = CAAGATCAGCTAAGAT-1

using 110 reads

====================================================================================

graph has 110 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 3, 5, 6, 16, 18, 24, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.46 = CAAGATCAGCTAGTGG-1

using 191 reads

====================================================================================

graph has 168 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 5, 10^2, 19, 39, 41, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.47 = CAAGATCAGCTCAACT-1

using 159 reads

====================================================================================

graph has 92 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 3^4, 4, 8, 20, 32^2, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.48 = CAAGATCAGCTCCTCT-1

using 192 reads

====================================================================================

graph has 170 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2, 3, 5, 6^2, 7, 9, 19, 20, 52, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.49 = CAAGATCAGCTTTGGT-1

using 347 reads

====================================================================================

graph has 220 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 18[2^3, 4^3, 8, 10, 12, 14, 15, 20, 21^2, 24, 48, 63, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.50 = CAAGATCAGGAATTAC-1

using 283 reads

====================================================================================

graph has 176 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2, 4, 5, 6, 7^2, 8, 11, 15, 20, 27, 32, 39, 45, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.51 = CAAGATCAGGCAGGTT-1

using 310 reads

====================================================================================

graph has 252 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^2, 3^2, 6^2, 13^2, 14, 15, 16, 23, 31, 34, 46, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.52 = CAAGATCAGGGAAACA-1

using 175 reads

====================================================================================

graph has 134 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 13[2, 3, 4^2, 5, 14^2, 17, 18, 19, 20, 24, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.53 = CAAGATCAGGGTTTCT-1

using 379 reads

====================================================================================

graph has 304 edges initially, 10 edges after simplification

total ucounts = 20
nonsolo ucounts = 19[2, 3^3, 5, 6, 7^3, 8, 13, 15, 18^2, 19, 37, 52, 77, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.55 = CAAGATCAGTAGGCCA-1

using 25 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.57 = CAAGATCAGTCCAGGA-1

using 182 reads

====================================================================================

graph has 96 edges initially, 8 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3, 4, 5, 19, 22, 27^2, 33, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.58 = CAAGATCAGTCCTCCT-1

using 265 reads

====================================================================================

graph has 244 edges initially, 10 edges after simplification

total ucounts = 22
nonsolo ucounts = 21[2, 3^4, 4^2, 5^3, 6^2, 8^2, 9, 10, 15, 18, 37, 53, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.59 = CAAGATCAGTCGTTTG-1

using 49 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^3, 6^2, 9^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.61 = CAAGATCAGTTACCCA-1

using 29 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.64 = CAAGATCAGTTTAGGA-1

using 80000 reads

====================================================================================

graph has 31875 edges initially, 776 edges after simplification

total ucounts = 2232
nonsolo ucounts = 1828[2^181, 3^139, 4^118, 5^91, 6^73, 7^67, 8^64, 9^63, 10^59, 11^63, 12^59, 13^58, 14^50, 15^43, 16^41, 17^43, 18^35, 19^31, 20^35, 21^26, 22^28, 23^20, 24^18, 25^18, 26^26, 27^22, 28^27, 29^13, 30^17, 31^12, 32^21, 33^17, 34^12, 35^15, 36^10, 37^2, 38^11, 39^6, 40^6, 41^6, 42^4, 43^6, 44^12, 45^12, 46^13, 47^6, 48^7, 50^5, 51^9, 52^7, 53^6, 54^3, 55^3, 56^4, 57^2, 58^2, 59^3, 60, 61^3, 62^7, 63^2, 65^3, 66^4, 67, 68^2, 69^3, 70^2, 71, 72, 73^2, 74^4, 75^2, 78^4, 79, 81, 82, 85, 91, 92, 101, 102^2, 103, 104, 116, 131, 197, 230, 343, 419, 437, 641, 674, 723, 887, 925, 1107, 1155, 1369, 1412, 1485, 1603, 1758, 1853, 2092, 2184, 7696, 10119, 10485]
surviving nonsolo ucounts = 20[343, 419, 437, 641, 674, 723, 887, 925, 1107, 1155, 1369, 1412, 1485, 1603, 1853, 2092, 2184, 7696, 10119, 10485]
ids = [1993, 1185, 1767, 9, 1148, 818, 1239, 1994, 1788, 1297, ...]

====================================================================================

UMI info for barcode CAAGATCAGTTTAGGA-1 contig 1 = AGGACTGAGC...
umi CAAACGCTTA = 1601 reads: +394 validated
umi GCATTTACAA = 671 reads: +394 validated
umi GCTTGCACGA = 892 reads: +394 validated
umi GCTTTCGCCC = 500 reads: -364 +1 -2XX +4 -1XX +2 -3XX +3 -3XX +1 -1XX +1 -2XX +5 -1XX invalidated
umi GGGCAAAGGC = 1175 reads: +394 validated
umi TTAAGGTATC = 344 reads: +371 -5 +18 non-validated

UMI info for barcode CAAGATCAGTTTAGGA-1 contig 2 = ATCAATGCAC...
umi AAGCGATCGT = 633 reads: +397 validated
umi CACACTACTA = 1486 reads: +397 validated
umi CCCACACGCG = 1839 reads: +397 validated
umi CTAATTTTAA = 715 reads: +397 validated
umi GCACGTTTGT = 2182 reads: +397 validated
umi GCCGCTCGTA = 421 reads: +397 validated
umi TCCTAGTAAT = 7732 reads: -126X +1 -15X +255 invalidated
umi TCCTCAGCTA = 10524 reads: -142X +255 invalidated
umi TCGCGTCTGC = 432 reads: +397 validated
umi TCGGTATTAC = 1104 reads: +397 validated
umi TTTCACGCCA = 1364 reads: +397 validated
umi TTTCTCTCGC = 1389 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=583]
0-147 ==> 0-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
147-484 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=5)
482-541 ==> 7-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
541-583 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 250 reads
cdr3 = CASRGGATNKLIF at 471, score = 6 + 8
umis assigned: [187, 1148, 1239, 1240, 1297, 1993]
of which 5 are surviving nonsolos
reads assigned: 5091
start codons at 147, 182, 259, 267, 361
confident = true

TIG 2[bases=495]
0-46 ==> 0-46 on |661|TRBV6-6|5'UTR| [len=46] (mis=0)
46-390 ==> 0-344 on |662|TRBV6-6|L-REGION+V-REGION| [len=344] (mis=6)
393-443 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 12 umis using 2448 reads
cdr3 = CASSAEDRYNEQFF at 373, score = 9 + 8
umis assigned: [9, 224, 450, 818, 1124, 1185, 1731, 1732, 1767, 1788] and 2 others
of which 12 are surviving nonsolos
reads assigned: 29356
start codons at 4, 46, 104, 157, 181, 196, 223, 287, 401
confident = true

REJECT CONTIGS

TIG 1[bases=309]
8-208 ==> 5800-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
217-257 ==> 7-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
257-309 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [1333, 1691, 1994]
of which 3 are surviving nonsolos
reads assigned: 12756
start codons at 85, 95, 169
confident = false
did not find CDR3
now this is a cell
paired!

TCCCGGGCAGCAGACACTGCTTCTTACTTCTGTGCTTCTCGTGGTGGTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGTGCCGAGGACAGGTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.69 = CAAGATCCAAGGTTTC-1

using 33 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 12, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.70 = CAAGATCCAAGTCTAC-1

using 135 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[135]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.71 = CAAGATCCAAGTTGTC-1

using 138 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[138]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.72 = CAAGATCCAATGCCAT-1

using 13122 reads

====================================================================================

graph has 4685 edges initially, 96 edges after simplification

total ucounts = 222
nonsolo ucounts = 160[2^18, 3^19, 4^12, 5^14, 6^6, 7^4, 8^8, 9^6, 10^6, 11^3, 12^3, 13^4, 14^7, 15^3, 16, 18^6, 19^4, 20^2, 22^2, 23^2, 24^4, 25, 26^2, 31^4, 33, 34, 35, 36^2, 39, 42, 56, 57, 69, 126, 132, 151, 359, 400, 1938, 2122, 2286, 3765]
surviving nonsolo ucounts = 7[151, 359, 400, 1938, 2122, 2286, 3765]
ids = [50, 132, 184, 15, 106, 111, 113]

====================================================================================

UMI info for barcode CAAGATCCAATGCCAT-1 contig 1 = AGAACTGGCA...
umi CAAGACGCAA = 114 reads: -389X +1 -5XX +2 invalidated
umi GACGCGCCCT = 2133 reads: +397 validated
umi GATATTACGG = 2278 reads: +397 validated
umi GGGTGACCAG = 315 reads: -382X +7 -1XX +2 -3XX +2 invalidated
umi TGGAGTGGAT = 399 reads: +397 validated

UMI info for barcode CAAGATCCAATGCCAT-1 contig 2 = TGGGGGCAGA...
umi ACACCAGTCC = 1948 reads: +403 validated
umi GATCACCGTA = 3827 reads: +28 -1XX +1 -4XX +2 -7XX +1 -2XX +2 -2XX +1 -1XX +351 invalidated
umi GTTAGTATGC = 1 reads: -390X +1 -4X +1 -1X +1 -1X +1 -3X invalidated

GOOD CONTIGS

TIG 1[bases=479]
0-30 ==> 13-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
30-374 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=4)
380-427 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
427-479 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 330 reads
cdr3 = CASSPFLESYEQYF at 357, score = 7 + 8
umis assigned: [50, 106, 111, 132, 184]
of which 5 are surviving nonsolos
reads assigned: 5160
start codons at 22, 30, 52, 165, 172, 180, 234, 238, 262
confident = true

TIG 2[bases=461]
16-359 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=6)
359-419 ==> 0-60 on |397|TRAJ12|J-REGION| [len=60] (mis=3)
419-461 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 401 reads
cdr3 = CVAPGGDSSYKLIF at 346, score = 6 + 9
umis assigned: [15, 113, 141]
of which 2 are surviving nonsolos
reads assigned: 5625
start codons at 16, 74, 144, 226, 278
confident = true
now this is a cell
paired!

CAGCTCAGCGATTCAGCCTCCTACATCTGTGTGGCCCCGGGAGGGGATAGCAGCTATAAATTGATCTTCGGGAGTGGGACCAGACTGCTGGTCAGGCCTG <==> CCCAGCCCCAACCAGACCTCTCTGTACTTCTGTGCCAGCAGTCCCTTCTTGGAGTCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.77 = CAAGATCCACCTCGGA-1

using 36 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.78 = CAAGATCCACGGCTAC-1

using 243 reads

====================================================================================

graph has 138 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 9, 19, 20, 25, 50, 52, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.80 = CAAGATCCACTGTCGG-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.85 = CAAGATCCAGCCAATT-1

using 44 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[4, 5, 6, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.91 = CAAGATCCAGGTTTCA-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.94 = CAAGATCCATCCGTGG-1

using 2507 reads

====================================================================================

graph has 892 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[2502]
surviving nonsolo ucounts = 1[2502]
ids = [3]

====================================================================================

UMI info for barcode CAAGATCCATCCGTGG-1 contig 1 = TTGGGGAAAG...
umi ATCGCCATTT = 2511 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=486]
34-381 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
387-434 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
434-486 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 165 reads
cdr3 = CASSLREGIYEQYF at 364, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 2463
start codons at 34, 143
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.96 = CAAGATCCATCTACGA-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.97 = CAAGATCCATGAAGTA-1

using 105 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[34, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.103 = CAAGATCGTAGAGTGC-1

using 76 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.106 = CAAGATCGTATAGGGC-1

using 72 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.109 = CAAGATCGTCAGAGGT-1

using 39 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.134 = CAAGATCGTTTAGGAA-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.136 = CAAGATCTCAAGGCTT-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.139 = CAAGATCTCAGCAACT-1

using 29 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.142 = CAAGATCTCATGCATG-1

using 42 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^3, 15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.143 = CAAGATCTCATGGTCA-1

using 54 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.145 = CAAGATCTCCACGAAT-1

using 44 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.149 = CAAGATCTCCCGGATG-1

using 102 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[13^2, 15, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.155 = CAAGATCTCGGATGGA-1

using 56 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.158 = CAAGATCTCGTCTGCT-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.160 = CAAGATCTCGTTTGCC-1

using 45 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.163 = CAAGATCTCTAGCACA-1

using 68 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.165 = CAAGATCTCTCCGGTT-1

using 29435 reads

====================================================================================

graph has 15049 edges initially, 372 edges after simplification

total ucounts = 1269
nonsolo ucounts = 970[2^141, 3^93, 4^74, 5^67, 6^51, 7^50, 8^45, 9^42, 10^30, 11^40, 12^33, 13^29, 14^27, 15^23, 16^22, 17^17, 18^18, 19^16, 20^16, 21^13, 22^13, 23^10, 24^13, 25^8, 26^6, 27^7, 28^7, 29^7, 30^6, 31^4, 32^2, 34^2, 35, 36^2, 37^2, 39^2, 40^2, 42, 43, 45^2, 47, 48, 51, 53, 55, 56^2, 62, 64, 71, 83, 90, 94, 98, 107^2, 1032, 1060, 1180, 1499, 1526, 1571, 2082, 2101, 6537]
surviving nonsolo ucounts = 8[1060, 1180, 1499, 1526, 1571, 2082, 2101, 6537]
ids = [696, 109, 645, 67, 113, 79, 1236, 126]

====================================================================================

UMI info for barcode CAAGATCTCTCCGGTT-1 contig 1 = GGCCCTCATC...
umi AATGCATTCT = 1535 reads: +400 validated
umi ACCCCATGGA = 1571 reads: +400 validated
umi GCACATGTAT = 1006 reads: -376 +4 -1XX +2 -1XX +6 -3XX +5 -1XX +1 invalidated
umi TTTCAACGTA = 2031 reads: -320X +1 -2XX +1 -2XX +1 -1XX +1 -1XX +5 -2XX +1 -3XX +1 -4XX +1 -2XX +1 -3XX +47 invalidated

UMI info for barcode CAAGATCTCTCCGGTT-1 contig 2 = CTGGAAGACC...
umi ACCAACCTGG = 1177 reads: +424 validated
umi GAACCATTAC = 1512 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=523]
71-418 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4)
423-471 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
471-523 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 176 reads
cdr3 = CASSPRGLQETQYF at 401, score = 7 + 7
umis assigned: [67, 113, 696, 1236]
of which 4 are surviving nonsolos
reads assigned: 6051
start codons at 71, 95, 180, 279, 392
confident = true

TIG 2[bases=511]
0-45 ==> 0-45 on |484|TRAV23/DV6|5'UTR| [len=45] (mis=0)
45-409 ==> 0-364 on |485|TRAV23/DV6|L-REGION+V-REGION| [len=364] (mis=1)
412-469 ==> 9-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 152 reads
cdr3 = CAASRRGGADGLTF at 396, score = 9 + 8
umis assigned: [109, 645]
of which 2 are surviving nonsolos
reads assigned: 2639
start codons at 45, 92, 202, 230, 286, 360
confident = true

REJECT CONTIGS

TIG 1[bases=369]
45-267 ==> 0-222 on segment before TRBJ1-5 exon 1 [len=222] (mis=0)
267-317 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
317-369 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [126]
of which 1 are surviving nonsolos
reads assigned: 6429
start codons at 47, 152, 254, 257, 293
confident = false
did not find CDR3

TIG 2[bases=515]
0-77 ==> 20-97 on |492|TRAV27|5'UTR| [len=97] (mis=0)
77-405 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=2)
411-473 ==> 0-62 on |423|TRAJ37|J-REGION| [len=62] (mis=2)
473-515 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [79, 760]
of which 1 are surviving nonsolos
reads assigned: 2067
start codons at 50, 59, 77, 121, 229, 330
confident = false
frameshifted full length stopped transcript of length 515
did not find CDR3
now this is a cell
paired!

CAGCCTGGAGACTCAGCCACCTACTTCTGTGCAGCAAGCAGAAGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCT <==> ACAGAGCAGGGGGACTCGGCCATGTATCTCTGTGCCAGCAGCCCAAGGGGACTCCAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.166 = CAAGATCTCTCCTATA-1

using 48 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 5, 8, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.168 = CAAGATCTCTTAGCCC-1

using 9685 reads

====================================================================================

graph has 2888 edges initially, 50 edges after simplification

total ucounts = 217
nonsolo ucounts = 131[2^23, 3^12, 4^8, 5^11, 6^12, 7^6, 8^5, 9^5, 10^10, 11^3, 12^2, 13^4, 14^4, 15, 16^2, 17^4, 18^4, 19^2, 20^2, 22^2, 23, 24, 29, 35, 40, 56, 81, 394, 7975]
surviving nonsolo ucounts = 2[394, 7975]
ids = [167, 117]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.170 = CAAGATCTCTTGCCGT-1

using 78 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.171 = CAAGGCCAGAACAACT-1

using 60 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[10, 11, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.172 = CAAGGCCAGACCCACC-1

using 131 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[131]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.181 = CAAGGCCAGCTACCTA-1

using 101 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.187 = CAAGGCCAGGATCGCA-1

using 68 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.188 = CAAGGCCAGGATGTAT-1

using 47 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.193 = CAAGGCCAGGTGACCA-1

using 78 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3, 4^2, 6, 7, 11, 13, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.198 = CAAGGCCAGTATCGAA-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.199 = CAAGGCCAGTCATCCA-1

using 29 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.204 = CAAGGCCAGTTTAGGA-1

using 24 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.205 = CAAGGCCCAAACGCGA-1

using 1617 reads

====================================================================================

graph has 648 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 1607]
surviving nonsolo ucounts = 1[1607]
ids = [0]

====================================================================================

UMI info for barcode CAAGGCCCAAACGCGA-1 contig 1 = CGATGATGGA...
umi AACTAGGTCC = 1607 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=509]
0-70 ==> 0-70 on |465|TRAV13-2|5'UTR| [len=70] (mis=0)
70-410 ==> 0-340 on |466|TRAV13-2|L-REGION+V-REGION| [len=340] (mis=2)
407-467 ==> 0-60 on |437|TRAJ5|J-REGION| [len=60] (mis=4)
467-509 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 87 reads
cdr3 = CAETTTGRRALTF at 397, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1579
start codons at 2, 5, 20, 41, 67, 70, 94, 289
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.207 = CAAGGCCCAACACCTA-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.209 = CAAGGCCCAACTGCGC-1

using 41 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^3, 6, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.210 = CAAGGCCCAAGCTGTT-1

using 106 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.212 = CAAGGCCCACACAGAG-1

using 67 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.215 = CAAGGCCCACATGACT-1

using 75 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[14, 15, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.216 = CAAGGCCCACCCATTC-1

using 55 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.218 = CAAGGCCCACGGCTAC-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.219 = CAAGGCCCACTCGACG-1

using 14 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.221 = CAAGGCCCAGACACTT-1

using 83 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 4^2, 8, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.230 = CAAGGCCCAGTGAGTG-1

using 1017 reads

====================================================================================

graph has 820 edges initially, 36 edges after simplification

total ucounts = 178
nonsolo ucounts = 103[2^29, 3^11, 4^12, 5^9, 6^5, 7^5, 8^3, 10^3, 12^2, 13, 14, 16, 17^3, 19^2, 20, 21, 22, 23, 24^3, 25, 28, 30, 31, 33, 35, 36, 45, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.236 = CAAGGCCCATCTCCCA-1

using 2099 reads

====================================================================================

graph has 664 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 17, 2076]
surviving nonsolo ucounts = 1[2076]
ids = [5]

====================================================================================

UMI info for barcode CAAGGCCCATCTCCCA-1 contig 1 = GAGAGTCCTG...
umi GCTTTAACTA = 2079 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=512]
0-66 ==> 0-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
66-410 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=0)
426-460 ==> 13-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
460-512 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 127 reads
cdr3 = CASSYSSSGTRYF at 393, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2048
start codons at 24, 52, 66, 124, 177, 201, 208, 216, 243, 307, 316
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.238 = CAAGGCCCATGGTTGT-1

using 25 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.240 = CAAGGCCCATTCTCAT-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.244 = CAAGGCCGTAAGTAGT-1

using 20 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.245 = CAAGGCCGTACACCGC-1

using 60 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.249 = CAAGGCCGTAGTACCT-1

using 153 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 24
nonsolo ucounts = 18[2^5, 3^3, 4, 5, 6, 7^2, 8, 9, 13^2, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.251 = CAAGGCCGTCAATGTC-1

using 48 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.254 = CAAGGCCGTCGTCTTC-1

using 22 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.255 = CAAGGCCGTCTCTTTA-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.261 = CAAGGCCGTGCAGGTA-1

using 43 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.263 = CAAGGCCGTTAAAGAC-1

using 42 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.264 = CAAGGCCGTTCCCGAG-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.268 = CAAGGCCGTTGACGTT-1

using 192 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 4, 5, 67, 108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.271 = CAAGGCCGTTGCGTTA-1

using 93 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 13, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.276 = CAAGGCCTCAACTCTT-1

using 8031 reads

====================================================================================

graph has 1428 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[15, 8011]
surviving nonsolo ucounts = 1[8011]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.278 = CAAGGCCTCACATACG-1

using 79 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.280 = CAAGGCCTCACTCCTG-1

using 48 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 4, 7, 8, 10, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.281 = CAAGGCCTCAGCATGT-1

using 50 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.284 = CAAGGCCTCAGTTTGG-1

using 96 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 6, 19, 28, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.285 = CAAGGCCTCATGCTCC-1

using 60 reads

====================================================================================

graph has 63 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 8, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.286 = CAAGGCCTCCACGACG-1

using 47 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.293 = CAAGGCCTCCTAGGGC-1

using 7216 reads

====================================================================================

graph has 4347 edges initially, 108 edges after simplification

total ucounts = 477
nonsolo ucounts = 335[2^62, 3^42, 4^28, 5^22, 6^17, 7^14, 8^17, 9^13, 10^7, 11^13, 12^15, 13^12, 14^7, 15^7, 16^6, 17^5, 18^5, 19^2, 20^4, 21^5, 22^3, 23^3, 24, 25^2, 26, 27, 28, 30^2, 31, 33, 34, 35^2, 36, 39, 44, 62, 64, 70^2, 72, 73, 98, 252, 694, 2811]
surviving nonsolo ucounts = 2[252, 2811]
ids = [308, 236]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.295 = CAAGGCCTCCTCATTA-1

using 61 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[21, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.296 = CAAGGCCTCGACCAGC-1

using 79 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^4, 3, 13, 23, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.297 = CAAGGCCTCGAGCCCA-1

using 224 reads

====================================================================================

graph has 130 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[5, 7, 13, 14, 42, 59, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.300 = CAAGGCCTCGCTGATA-1

using 53 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.310 = CAAGGCCTCTACTATC-1

using 90 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.312 = CAAGGCCTCTATCCCG-1

using 38 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4, 6, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.313 = CAAGGCCTCTCACATT-1

using 20 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.315 = CAAGGCCTCTCCAGGG-1

using 60 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.317 = CAAGGCCTCTCCTATA-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.318 = CAAGGCCTCTGATTCT-1

using 22 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.321 = CAAGGCCTCTGTGCAA-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.322 = CAAGGCCTCTTATCTG-1

using 79 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[12, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.332 = CAAGTTGAGACGACGT-1

using 287 reads

====================================================================================

graph has 342 edges initially, 6 edges after simplification

total ucounts = 57
nonsolo ucounts = 33[2^12, 3^5, 4^2, 5^2, 6^2, 7, 8, 9, 10, 11^2, 12, 14, 37, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.333 = CAAGTTGAGACTGTAA-1

using 2362 reads

====================================================================================

graph has 936 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^2, 6^2, 9, 12, 19, 60, 2242]
surviving nonsolo ucounts = 1[2242]
ids = [5]

====================================================================================

UMI info for barcode CAAGTTGAGACTGTAA-1 contig 1 = TGGGTCCCAT...
umi CTCATATCGC = 2245 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=476]
27-369 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=1)
376-424 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=3)
424-476 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 187 reads
cdr3 = CASSLHVVTEAFF at 357, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2207
start codons at 27, 169, 250
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.335 = CAAGTTGAGAGAGCTC-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.336 = CAAGTTGAGAGGGATA-1

using 1907 reads

====================================================================================

graph has 708 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[1905]
surviving nonsolo ucounts = 1[1905]
ids = [0]

====================================================================================

UMI info for barcode CAAGTTGAGAGGGATA-1 contig 1 = ATCCTGCCCT...
umi ACGTCAACCA = 1926 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=474]
0-19 ==> 20-39 on |672|TRBV7-2|5'UTR| [len=39] (mis=0)
19-366 ==> 0-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=1)
375-422 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
422-474 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 134 reads
cdr3 = CASSLGSGRTYEQYF at 349, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1874
start codons at 19, 128
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.338 = CAAGTTGAGATCTGCT-1

using 56 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.343 = CAAGTTGAGCATGGCA-1

using 33 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.344 = CAAGTTGAGCCACCTG-1

using 73663 reads

====================================================================================

graph has 40070 edges initially, 1595 edges after simplification

total ucounts = 2829
nonsolo ucounts = 2379[2^228, 3^169, 4^109, 5^95, 6^83, 7^69, 8^67, 9^65, 10^58, 11^56, 12^55, 13^46, 14^37, 15^31, 16^34, 17^37, 18^38, 19^37, 20^32, 21^20, 22^25, 23^30, 24^23, 25^31, 26^24, 27^21, 28^25, 29^24, 30^18, 31^13, 32^23, 33^10, 34^13, 35^19, 36^16, 37^16, 38^11, 39^15, 40^13, 41^17, 42^14, 43^17, 44^8, 45^11, 46^14, 47^12, 48^8, 49^13, 50^9, 51^12, 52^17, 53^10, 54^12, 55^8, 56^10, 57^10, 58^15, 59^7, 60^11, 61^9, 62^9, 63^12, 64^7, 65^10, 66^8, 67^10, 68^14, 69^8, 70^5, 71^6, 72^9, 73^8, 74^8, 75^10, 76^13, 77^6, 78^8, 79^9, 80^11, 81^6, 82^8, 83^13, 84^4, 85^11, 86^6, 87^6, 88^12, 89^7, 90^4, 91^6, 92^10, 93^5, 94^7, 95^5, 96^3, 97^8, 98^3, 99^5, 100^6, 101^7, 102^5, 103^4, 104^4, 105^4, 106^8, 107^6, 108^3, 109^6, 110, 111^5, 112, 113^2, 114^2, 116^2, 117, 118^4, 119^4, 120, 121, 122^2, 123^2, 124^2, 125^2, 126, 127^2, 133, 135, 137, 139, 143, 144, 148, 204, 238, 2010, 2223]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.345 = CAAGTTGAGCCACTAT-1

using 162 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4, 52, 103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.349 = CAAGTTGAGCTAGTTC-1

using 46 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.351 = CAAGTTGAGCTGTCTA-1

using 35 reads

====================================================================================

graph has 31 edges initially, 5 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.352 = CAAGTTGAGCTTATCG-1

using 49 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.356 = CAAGTTGAGGCAGTCA-1

using 73 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[33, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.358 = CAAGTTGAGGCTATCT-1

using 43 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.361 = CAAGTTGAGGGTCTCC-1

using 81 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.362 = CAAGTTGAGGTAGCCA-1

using 46 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.363 = CAAGTTGAGGTGATAT-1

using 1501 reads

====================================================================================

graph has 524 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[1498]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.364 = CAAGTTGAGGTTCCTA-1

using 61 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.367 = CAAGTTGAGTCAAGCG-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.368 = CAAGTTGAGTCATCCA-1

using 18870 reads

====================================================================================

graph has 11891 edges initially, 386 edges after simplification

total ucounts = 1134
nonsolo ucounts = 854[2^114, 3^71, 4^75, 5^46, 6^65, 7^45, 8^40, 9^32, 10^30, 11^25, 12^22, 13^27, 14^19, 15^19, 16^21, 17^16, 18^11, 19^15, 20^10, 21^11, 22^13, 23^15, 24^10, 25^13, 26^10, 27^4, 28^6, 29^8, 30^9, 31^3, 32^2, 33^5, 34, 35, 36, 37^2, 39^2, 40, 41, 42, 43, 44^2, 46^3, 47^2, 50^2, 52, 56^3, 57, 58, 60, 63, 65, 66^2, 71, 75, 80, 88, 90, 91^2, 96, 120, 213, 7974]
surviving nonsolo ucounts = 2[213, 7974]
ids = [893, 426]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.369 = CAAGTTGAGTCATGCT-1

using 182 reads

====================================================================================

graph has 212 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[3, 5, 31, 35, 37, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.371 = CAAGTTGAGTGAAGAG-1

using 1957 reads

====================================================================================

graph has 758 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[30, 1924]
surviving nonsolo ucounts = 1[1924]
ids = [1]

====================================================================================

UMI info for barcode CAAGTTGAGTGAAGAG-1 contig 1 = TGGGGACAGT...
umi ACGGGCCGCA = 1927 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=498]
52-399 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=3)
398-446 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=4)
446-498 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 150 reads
cdr3 = CASSLWEYGYTF at 382, score = 7 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1892
start codons at 52, 76, 161, 260, 373, 404
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.377 = CAAGTTGAGTTCCACA-1

using 4311 reads

====================================================================================

graph has 3137 edges initially, 90 edges after simplification

total ucounts = 755
nonsolo ucounts = 412[2^103, 3^77, 4^39, 5^21, 6^19, 7^24, 8^4, 9^11, 10^10, 11^5, 12^11, 13^6, 14^7, 15^7, 16^5, 17, 18^6, 19^2, 20, 21^2, 22^7, 23, 24, 25^3, 26^6, 27, 28^2, 29, 30^2, 31, 32, 33^2, 34, 35, 36, 39, 40, 42, 43^2, 44, 45, 49, 51, 52^2, 59, 61, 69, 71, 72, 74^2, 83^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.378 = CAAGTTGCAAAGAATC-1

using 162 reads

====================================================================================

graph has 144 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3, 4, 5, 8, 10, 21, 24, 25, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.382 = CAAGTTGCAAGCCATT-1

using 26 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.383 = CAAGTTGCAAGGACTG-1

using 13586 reads

====================================================================================

graph has 8614 edges initially, 248 edges after simplification

total ucounts = 860
nonsolo ucounts = 638[2^95, 3^72, 4^51, 5^28, 6^41, 7^28, 8^29, 9^32, 10^16, 11^26, 12^19, 13^27, 14^20, 15^14, 16^11, 17^11, 18^8, 19^8, 20^11, 21^4, 22^7, 23^11, 24^8, 25^3, 26^2, 27^3, 28^5, 29^5, 30^3, 31^2, 32^2, 33, 34^2, 35^2, 36, 37, 39, 40, 41, 42, 45^2, 46, 47, 49^3, 51, 52, 53, 54, 56^2, 59, 60, 61, 62, 63, 71, 72^2, 75, 84, 93, 6036]
surviving nonsolo ucounts = 1[6036]
ids = [59]

====================================================================================

REJECT CONTIGS

TIG 1[bases=309]
5-56 ==> 53-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
56-105 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
105-207 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=1)
257-309 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [59, 600]
of which 1 are surviving nonsolos
reads assigned: 5970
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.387 = CAAGTTGCAATCAGAA-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.389 = CAAGTTGCAATTCCTT-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.394 = CAAGTTGCACATCCGG-1

using 24792 reads

====================================================================================

graph has 9638 edges initially, 124 edges after simplification

total ucounts = 569
nonsolo ucounts = 414[2^85, 3^45, 4^34, 5^26, 6^21, 7^25, 8^13, 9^16, 10^19, 11^14, 12^11, 13^14, 14^5, 15^7, 16^10, 17^4, 18^7, 19^2, 20, 21^10, 22^2, 23^4, 24^2, 25^3, 27, 28, 30^2, 32, 33, 34, 37, 39^2, 40, 41, 42, 49, 52, 53, 54, 58^2, 83, 147, 496, 675, 859, 1233, 1331, 1448, 1468, 1526, 1601, 2341, 2396, 2507, 2916]
surviving nonsolo ucounts = 12[496, 675, 859, 1233, 1331, 1448, 1468, 1601, 2341, 2396, 2507, 2916]
ids = [444, 477, 245, 138, 116, 371, 64, 277, 478, 164, ...]

====================================================================================

UMI info for barcode CAAGTTGCACATCCGG-1 contig 1 = GGAGTTTCTT...
umi AATCCGGGGA = 2695 reads: -369 +2 -1XX +2 -2XX +1 -1XX +2 -1XX +2 -1XX +2 -2XX +1 -2XX +6 -2XX +2 -2XX invalidated
umi ACGCTCACCT = 1393 reads: -376X +1 -1X +2 -1XX +2 -1XX +2 -2XX +1 -2XX +6 -2XX +2 -2XX invalidated
umi CAACATTGCG = 1236 reads: +403 validated
umi GTTACAGTCG = 1365 reads: -381X +2 -1XX +2 -2XX +1 -2XX +6 -2XX +2 -2XX invalidated
umi TCGGCACACA = 493 reads: +403 validated
umi TGCAGGCCTA = 642 reads: -376X +1 -1XX +2 -1XX +2 -1XX +2 -2XX +1 -2XX +6 -2XX +2 -2XX invalidated

UMI info for barcode CAAGTTGCACATCCGG-1 contig 2 = GTCATCCCTC...
umi ACCTGACCTT = 2523 reads: +391 validated
umi ATCGGCGCGT = 1327 reads: +391 validated
umi CATTAGACAT = 2396 reads: +391 validated
umi CTGACCGTCA = 859 reads: +391 validated
umi GAGTTTGGCG = 1617 reads: +391 validated
umi TGCATTATCC = 2340 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=681]
236-577 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=1)
576-639 ==> 0-63 on |425|TRAJ39|J-REGION| [len=63] (mis=1)
639-681 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CAVGPNNNAGNMLTF at 563, score = 7 + 9
umis assigned: [24, 64, 138, 371, 444, 477]
of which 6 are surviving nonsolos
reads assigned: 7786
start codons at 236, 358, 372, 399, 546, 585, 596, 626
confident = true

TIG 2[bases=521]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=5)
421-469 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
469-521 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 623 reads
cdr3 = CSAPRPGQQETQYF at 399, score = 7 + 7
umis assigned: [56, 116, 164, 245, 277, 478]
of which 6 are surviving nonsolos
reads assigned: 10840
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true
now this is a cell
paired!

TGGAGTGATGCTGCTGAGTACTTCTGTGCTGTGGGTCCGAATAATAATGCAGGCAACATGCTCACCTTTGGAGGGGGAACAAGGTTAATGGTCAAACCCC <==> GCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCCCCACGACCGGGACAGCAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.401 = CAAGTTGCACTTCGAA-1

using 260 reads

====================================================================================

graph has 337 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 11, 28, 51, 74, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.403 = CAAGTTGCAGACAAGC-1

using 12406 reads

====================================================================================

graph has 6155 edges initially, 120 edges after simplification

total ucounts = 561
nonsolo ucounts = 399[2^55, 3^44, 4^22, 5^29, 6^17, 7^21, 8^16, 9^20, 10^18, 11^18, 12^13, 13^12, 14^9, 15^11, 16^7, 17^3, 18^6, 19^5, 20^10, 21^5, 22^7, 23^3, 24^2, 25^4, 26^2, 27^3, 29^2, 30^4, 32^3, 33, 34^2, 37, 38, 39, 40, 43, 44, 45, 47^2, 50^2, 51^2, 52, 54, 58, 64, 66^2, 70, 72, 87, 95, 98, 7317]
surviving nonsolo ucounts = 1[7317]
ids = [386]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.406 = CAAGTTGCAGATCTGT-1

using 148 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 4, 19, 29, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.408 = CAAGTTGCAGGAACGT-1

using 3220 reads

====================================================================================

graph has 2432 edges initially, 50 edges after simplification

total ucounts = 278
nonsolo ucounts = 192[2^34, 3^26, 4^17, 5^13, 6^12, 7^5, 8^10, 9^5, 10^7, 11^4, 12^2, 13^3, 14^7, 15^7, 16^5, 17^4, 18^5, 19^5, 21^3, 22^3, 23, 24^3, 34, 36, 41, 42, 46, 49, 52, 65, 67, 76, 1178]
surviving nonsolo ucounts = 2[15, 1178]
ids = [136, 94]

====================================================================================

UMI info for barcode CAAGTTGCAGGAACGT-1 contig 1 = GGGGAGTCAC...
umi CCAATAGTCA = 1181 reads: +412 validated
umi TTCAGCGTCA = 3 reads: -399X +1 -2XX +2 -6XX +1 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=573]
119-469 ==> 0-350 on |505|TRAV38-1|L-REGION+V-REGION| [len=350] (mis=1)
465-531 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=3)
531-573 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CAFMKLSGGSNYKLTF at 452, score = 7 + 9
umis assigned: [94, 256]
of which 1 are surviving nonsolos
reads assigned: 1162
start codons at 119, 176, 209, 252, 311, 351, 443, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.410 = CAAGTTGCAGGCTGAA-1

using 2757 reads

====================================================================================

graph has 882 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[2756]
surviving nonsolo ucounts = 1[2756]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=428]
14-61 ==> 8686-8733 on segment before TRBV7-9 exon 1 [len=8733] (mis=0)
61-180 ==> 0-119 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
180-323 ==> 204-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=4) [SHIFT!]
329-376 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
376-428 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSSRGGWREQYF at 306, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 2714
start codons at 8, 61, 85, 170, 184, 297
confident = false
not full
frameshifted full length stopped transcript of length 428
VJ delta = 101
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.412 = CAAGTTGCAGGTCGTC-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.416 = CAAGTTGCATACGCCG-1

using 64 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.418 = CAAGTTGCATCTCGCT-1

using 48 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.421 = CAAGTTGCATGCCTAA-1

using 10203 reads

====================================================================================

graph has 1704 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 3[6, 10, 10177]
surviving nonsolo ucounts = 1[10177]
ids = [12]

====================================================================================

REJECT CONTIGS

TIG 1[bases=315]
5-62 ==> 47-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
62-111 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
111-213 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
263-315 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 10057
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.425 = CAAGTTGCATTTGCCC-1

using 91 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.426 = CAAGTTGCATTTGCTT-1

using 42 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.427 = CAAGTTGGTACCGTAT-1

using 61 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.432 = CAAGTTGGTAGGCATG-1

using 1093 reads

====================================================================================

graph has 410 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[1092]
surviving nonsolo ucounts = 1[1092]
ids = [1]

====================================================================================

UMI info for barcode CAAGTTGGTAGGCATG-1 contig 1 = CTCTTCATTC...
umi GGCACCGGGT = 1102 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=564]
0-121 ==> 87-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
121-465 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=2)
474-512 ==> 10-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
512-564 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CASSIGTNLYTF at 448, score = 6 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1078
start codons at 121, 179, 230, 266, 271, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.435 = CAAGTTGGTATCGCAT-1

using 13 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.436 = CAAGTTGGTCAAACTC-1

using 171 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[171]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.439 = CAAGTTGGTCACCCAG-1

using 14 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.440 = CAAGTTGGTCAGCTAT-1

using 16 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.442 = CAAGTTGGTCCAGTTA-1

using 245 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[245]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.447 = CAAGTTGGTCGAGTTT-1

using 6386 reads

====================================================================================

graph has 2994 edges initially, 76 edges after simplification

total ucounts = 322
nonsolo ucounts = 223[2^38, 3^21, 4^12, 5^19, 6^15, 7^14, 8^7, 9^9, 10^9, 11^10, 12^5, 13, 14^7, 15^5, 16^9, 17^2, 18^6, 19^4, 20, 21^3, 22, 23^2, 24, 25, 28, 30^3, 31^2, 36^2, 38, 39, 41, 42, 44, 46, 59, 60, 62, 70, 87, 88, 3744]
surviving nonsolo ucounts = 1[3744]
ids = [160]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.452 = CAAGTTGGTCTCTCTG-1

using 53 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.460 = CAAGTTGGTGTTCTTT-1

using 72 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.464 = CAAGTTGGTTATCCGA-1

using 79 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.466 = CAAGTTGGTTCAGTAC-1

using 35987 reads

====================================================================================

graph has 14636 edges initially, 350 edges after simplification

total ucounts = 1026
nonsolo ucounts = 775[2^115, 3^74, 4^65, 5^44, 6^43, 7^32, 8^39, 9^19, 10^30, 11^31, 12^26, 13^20, 14^16, 15^17, 16^11, 17^13, 18^14, 19^12, 20^13, 21^10, 22^4, 23^8, 24^10, 25^13, 26^16, 27^7, 28^6, 29^2, 30, 31^3, 32^3, 33^2, 34^2, 35^3, 36, 37^4, 38^2, 39, 40^2, 41, 42^2, 43^2, 44, 45^2, 46, 48, 49, 50, 52, 54, 55, 57, 59, 62^2, 70, 91, 105, 107, 114, 458, 529, 915, 917, 1065, 1093, 1212, 1461, 1470, 1740, 1838, 1874, 2242, 2327, 2396, 2420, 2912]
surviving nonsolo ucounts = 17[458, 529, 915, 917, 1065, 1093, 1212, 1461, 1470, 1740, 1838, 1874, 2242, 2327, 2396, 2420, 2912]
ids = [6, 828, 971, 665, 119, 260, 711, 60, 551, 936, ...]

====================================================================================

UMI info for barcode CAAGTTGGTTCAGTAC-1 contig 1 = AGAATGCTTA...
umi AATATAGCCT = 1470 reads: +400 validated
umi ATCCGGTGGC = 1088 reads: +400 validated
umi GTATAGACCA = 589 reads: +383 -10 +7 non-validated
umi TAAGGGCTTT = 1221 reads: +400 validated
umi TCAATCGGCC = 1873 reads: +400 validated
umi TCGTCCTTCT = 1852 reads: +400 validated
umi TCTTCATGCC = 2403 reads: +400 validated
umi TCTTGTGCTA = 2436 reads: +400 validated
umi TGCCCGCGCA = 2349 reads: +400 validated
umi TTAGGATTCC = 2896 reads: +400 validated

UMI info for barcode CAAGTTGGTTCAGTAC-1 contig 2 = ACTATTTCTA...
umi GACACATTTC = 1459 reads: +376 validated
umi TCCTGTTCTC = 480 reads: +376 validated

UMI info for barcode CAAGTTGGTTCAGTAC-1 contig 3 = TGGGGACTCT...
umi ACCCCTCCAA = 1067 reads: +412 validated
umi TCACGTGTTG = 2252 reads: +412 validated
umi TTCACGGCAC = 1760 reads: +412 validated
umi TTGGATGATC = 917 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=2)
398-447 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
447-499 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 1259 reads
cdr3 = CASSVRLAGTDTQYF at 374, score = 9 + 8
umis assigned: [60, 260, 665, 711, 787, 841, 860, 865, 878, 923]
of which 10 are surviving nonsolos
reads assigned: 17874
start codons at 3, 47, 169, 255
confident = true

TIG 2[bases=494]
0-76 ==> 0-76 on |487|TRAV25|5'UTR| [len=76] (mis=0)
76-403 ==> 0-327 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=2)
404-452 ==> 15-63 on |435|TRAJ48|J-REGION| [len=63] (mis=0)
452-494 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 89 reads
cdr3 = CAGDEKLTF at 394, score = 6 + 10
umis assigned: [551, 828]
of which 2 are surviving nonsolos
reads assigned: 1912
start codons at 58, 76, 94, 105, 109, 128, 142, 164, 377, 404
confident = true

TIG 3[bases=483]
29-371 ==> 0-342 on |505|TRAV38-1|L-REGION+V-REGION| [len=350] (mis=0)
375-441 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=0)
441-483 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 386 reads
cdr3 = CAFLAYSGAGSYQLTF at 362, score = 7 + 10
umis assigned: [119, 794, 936, 971]
of which 4 are surviving nonsolos
reads assigned: 5893
start codons at 29, 86, 119, 162, 221, 261, 353
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.467 = CAAGTTGGTTCCACAA-1

using 31 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.468 = CAAGTTGGTTGATTCG-1

using 69 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 5, 7^2, 8, 10, 12, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.473 = CAAGTTGTCAAACAAG-1

using 1952 reads

====================================================================================

graph has 570 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^3, 4, 5, 1933]
surviving nonsolo ucounts = 1[1933]
ids = [9]

====================================================================================

REJECT CONTIGS

TIG 1[bases=413]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |560|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=5)
361-413 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 1882
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.476 = CAAGTTGTCAACGCTA-1

using 65 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.480 = CAAGTTGTCACGATGT-1

using 301 reads

====================================================================================

graph has 224 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[3, 4^2, 6^2, 7, 9^2, 12, 16, 26, 53, 70, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.482 = CAAGTTGTCACTATTC-1

using 66 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.483 = CAAGTTGTCACTGGGC-1

using 23 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.484 = CAAGTTGTCACTTATC-1

using 113 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.492 = CAAGTTGTCATGGTCA-1

using 123 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[30, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.497 = CAAGTTGTCCCTGACT-1

using 40 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.499 = CAAGTTGTCCGCGTTT-1

using 32 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.500 = CAAGTTGTCCTAGAAC-1

using 104 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 13, 14, 19, 24, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.501 = CAAGTTGTCCTTCAAT-1

using 36 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.502 = CAAGTTGTCGACAGCC-1

using 1339 reads

====================================================================================

graph has 404 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 4, 5, 1326]
surviving nonsolo ucounts = 1[1326]
ids = [4]

====================================================================================

UMI info for barcode CAAGTTGTCGACAGCC-1 contig 1 = AGTGGTCACA...
umi TTCTATTGGG = 1327 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=498]
0-49 ==> 29-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
49-384 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=5)
396-446 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 78 reads
cdr3 = CSATRAATRAKNIQYF at 370, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 1304
start codons at 49, 187, 223, 229, 242, 302
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.505 = CAAGTTGTCGTATCAG-1

using 95 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 6, 37, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.507 = CAAGTTGTCGTTACGA-1

using 915 reads

====================================================================================

graph has 368 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[7, 12, 13, 880]
surviving nonsolo ucounts = 1[880]
ids = [2]

====================================================================================

UMI info for barcode CAAGTTGTCGTTACGA-1 contig 1 = GGAAGCCTGT...
umi GATTAAAAGA = 883 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=600]
0-157 ==> 51-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
157-501 ==> 0-344 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=5)
506-548 ==> 7-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
548-600 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CASSSMGNTQYF at 484, score = 6 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 869
start codons at 157, 215, 266, 302, 307, 371, 499
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.510 = CAAGTTGTCTACTTAC-1

using 56 reads

====================================================================================

graph has 65 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[4^2, 5, 6, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.511 = CAAGTTGTCTAGCACA-1

using 14295 reads

====================================================================================

graph has 10813 edges initially, 320 edges after simplification

total ucounts = 1240
nonsolo ucounts = 879[2^128, 3^91, 4^89, 5^53, 6^42, 7^49, 8^41, 9^28, 10^38, 11^31, 12^20, 13^22, 14^24, 15^27, 16^17, 17^15, 18^12, 19^12, 20^5, 21^10, 22^12, 23^4, 24^8, 25^7, 26^5, 27^4, 28^5, 29^4, 30^6, 31^5, 32^2, 33^2, 34^6, 35^3, 36, 37^3, 39^2, 40^3, 41, 43^3, 46, 47, 48^2, 51^2, 52^3, 53, 55^2, 56^3, 57, 59, 60, 62, 63^2, 65, 69, 74, 75, 76^3, 78, 84, 85, 112, 116, 164, 173, 397, 761, 864, 1347]
surviving nonsolo ucounts = 7[84, 164, 173, 397, 761, 864, 1347]
ids = [602, 283, 584, 141, 529, 1149, 132]

====================================================================================

UMI info for barcode CAAGTTGTCTAGCACA-1 contig 1 = AGGACTGAGC...
umi ACCTCGCGAT = 1349 reads: +403 validated
umi TTACTACTCC = 818 reads: -372 +2 -3XX +6 -1XX +2 -2XX +1 -2XX +1 -2XX +2 -3XX +2 -2XX invalidated
umi TTGCCTTCCA = 2 reads: -389 +1 -3XX +1 -5XX +1 -3XX invalidated

GOOD CONTIGS

TIG 1[bases=592]
0-147 ==> 0-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
147-484 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=2)
484-550 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=5)
550-592 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CATAPGSGGGADGLTF at 471, score = 6 + 8
umis assigned: [132, 1149, 1193]
of which 2 are surviving nonsolos
reads assigned: 2139
start codons at 147, 182, 259, 267, 361
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.516 = CAAGTTGTCTGAGTGT-1

using 40 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.517 = CAAGTTGTCTGATTCT-1

using 85 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 5, 9, 11, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.518 = CAAGTTGTCTGCCAGG-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.524 = CAAGTTGTCTTGCAAG-1

using 87 reads

====================================================================================

graph has 71 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[13, 16, 17, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.527 = CACAAACAGAAACCTA-1

using 23944 reads

====================================================================================

graph has 14834 edges initially, 563 edges after simplification

total ucounts = 1238
nonsolo ucounts = 969[2^104, 3^82, 4^59, 5^57, 6^42, 7^33, 8^37, 9^33, 10^28, 11^28, 12^37, 13^32, 14^31, 15^20, 16^14, 17^14, 18^17, 19^18, 20^21, 21^11, 22^19, 23^17, 24^14, 25^16, 26^18, 27^18, 28^4, 29^15, 30^6, 31^9, 32^13, 33^4, 34^5, 35^9, 36^7, 37^3, 38^2, 39^5, 40^5, 41^2, 42^5, 43^3, 44, 45^2, 46^3, 47^2, 48^3, 50^2, 52^2, 53, 54, 56^2, 58, 59^2, 60^2, 61, 62, 63, 65, 66, 67, 70, 71, 72, 73, 77, 78, 80, 83, 85, 90^3, 106, 116, 141, 912, 1106, 1140, 1266, 1498, 1507, 1524]
surviving nonsolo ucounts = 7[912, 1106, 1140, 1266, 1498, 1507, 1524]
ids = [557, 257, 612, 922, 81, 622, 1052]

====================================================================================

UMI info for barcode CACAAACAGAAACCTA-1 contig 1 = AGCTCCTGTA...
umi ACAATCTCCA = 1502 reads: +409 validated
umi ATCGCACGTC = 1113 reads: +409 validated
umi CTATCATCAC = 1137 reads: +409 validated
umi CTATTTCCCC = 1513 reads: +409 validated
umi TCTCGTCTCA = 1526 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=525]
0-64 ==> 0-64 on |571|TRBV12-5|5'UTR| [len=64] (mis=0)
64-411 ==> 0-347 on |572|TRBV12-5|L-REGION+V-REGION| [len=347] (mis=1)
411-427 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
424-473 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=5)
473-525 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 403 reads
cdr3 = CASGLGGLAGVRDTQYF at 394, score = 8 + 8
umis assigned: [81, 257, 612, 622, 1052]
of which 5 are surviving nonsolos
reads assigned: 6660
start codons at 64, 122, 163, 181, 186, 235, 238, 287, 298, 325, 332
confident = true

REJECT CONTIGS

TIG 1[bases=473]
0-22 ==> 6466-6488 on segment before TRBV12-2 exon 1 [len=6488] (mis=0)
22-39 ==> 0-17 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=1)
39-318 ==> 20-299 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=54)
370-420 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
429-473 ==> 8-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [557]
of which 1 are surviving nonsolos
reads assigned: 694
start codons at 22, 69, 77, 104, 118, 141, 245, 253, 287, 344, 378
confident = false
see deletion of 3 bases at pos 17 on |566|TRBV12-3|L-REGION+V-REGION|
did not find CDR3

TIG 2[bases=461]
15-358 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=1)
360-419 ==> 0-59 on |448|TRAJ7|J-REGION| [len=59] (mis=4)
419-461 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [894, 922, 996]
of which 1 are surviving nonsolos
reads assigned: 1248
start codons at 15, 73, 143, 225, 277
confident = false
frameshifted full length stopped transcript of length 461
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.528 = CACAAACAGAACAACT-1

using 119 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 8, 11, 39, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.529 = CACAAACAGAACAATC-1

using 36 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.530 = CACAAACAGAAGAAGC-1

using 53849 reads

====================================================================================

graph has 12777 edges initially, 263 edges after simplification

total ucounts = 657
nonsolo ucounts = 470[2^74, 3^40, 4^36, 5^27, 6^25, 7^14, 8^15, 9^13, 10^13, 11^17, 12^12, 13^10, 14^14, 15^7, 16^10, 17^11, 18^5, 19^4, 20^11, 21^8, 22^4, 23^4, 24^7, 25^3, 26^4, 27^6, 28^7, 30^6, 31^3, 32, 33, 34, 35, 36^4, 37^2, 38^2, 39^2, 41, 42, 43, 44^4, 45^3, 46^2, 47^3, 48^3, 50^2, 53^3, 56^2, 58, 64, 65, 69, 79, 82, 83, 92^2, 93, 97^3, 1121, 4404, 4556, 5176, 5427, 7861, 9088, 9287]
surviving nonsolo ucounts = 8[1121, 4404, 4556, 5176, 5427, 7861, 9088, 9287]
ids = [423, 453, 635, 458, 415, 196, 246, 22]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.533 = CACAAACAGACAAGCC-1

using 31 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.535 = CACAAACAGACACTAA-1

using 76 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 4, 11, 15, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.539 = CACAAACAGAGTAAGG-1

using 106 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.542 = CACAAACAGATCCCGC-1

using 45 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[9, 10, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.544 = CACAAACAGATGGGTC-1

using 809 reads

====================================================================================

graph has 414 edges initially, 10 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[4^2, 5, 9, 28, 756]
surviving nonsolo ucounts = 1[756]
ids = [5]

====================================================================================

UMI info for barcode CACAAACAGATGGGTC-1 contig 1 = TGGGGACAGT...
umi CTGAGATACA = 765 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=498]
52-399 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
398-446 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CASSLALETQYF at 382, score = 7 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 744
start codons at 52, 76, 161, 260, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.547 = CACAAACAGCCCAATT-1

using 5640 reads

====================================================================================

graph has 3801 edges initially, 78 edges after simplification

total ucounts = 365
nonsolo ucounts = 258[2^35, 3^17, 4^27, 5^17, 6^9, 7^18, 8^11, 9^10, 10^12, 11^7, 12^8, 13^8, 14^6, 15^4, 16^9, 17^9, 18^7, 19^3, 20^3, 21^2, 22^3, 23^4, 25^6, 26^2, 27, 28, 29, 30, 32, 34^2, 35, 36^2, 37^2, 39, 42, 59, 70, 71, 74, 85, 98, 2445]
surviving nonsolo ucounts = 1[2445]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.553 = CACAAACAGCGTGTCC-1

using 55 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.555 = CACAAACAGCTCCTTC-1

using 31 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.556 = CACAAACAGCTCTCGG-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.561 = CACAAACAGGACATTA-1

using 38 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[14, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.563 = CACAAACAGGCTAGGT-1

using 39 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 5, 6, 7, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.566 = CACAAACAGGTACTCT-1

using 122 reads

====================================================================================

graph has 53 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[122]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.569 = CACAAACAGGTGTTAA-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.571 = CACAAACAGTACGTTC-1

using 90 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.572 = CACAAACAGTACTTGC-1

using 12026 reads

====================================================================================

graph has 10864 edges initially, 458 edges after simplification

total ucounts = 1209
nonsolo ucounts = 913[2^115, 3^97, 4^68, 5^58, 6^51, 7^32, 8^26, 9^33, 10^32, 11^34, 12^31, 13^19, 14^24, 15^20, 16^27, 17^29, 18^18, 19^12, 20^12, 21^16, 22^14, 23^13, 24^11, 25^14, 26^11, 27^14, 28^7, 29^10, 30^9, 31^6, 32^5, 33^4, 34^4, 35^3, 36^2, 37^4, 38, 39^4, 40^3, 41, 42^2, 43^3, 44^3, 53, 54, 56, 58, 59, 63, 68, 79, 88, 89, 663]
surviving nonsolo ucounts = 4[3^2, 20, 663]
ids = [267, 590, 172, 636]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.575 = CACAAACAGTATCTCG-1

using 102 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.576 = CACAAACAGTCCATAC-1

using 47 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 14, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.580 = CACAAACAGTGCCAGA-1

using 24 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.581 = CACAAACAGTGCGATG-1

using 34 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.582 = CACAAACAGTTATCGC-1

using 51 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.583 = CACAAACAGTTCGCAT-1

using 70 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 6, 8, 25, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.586 = CACAAACCAAATACAG-1

using 75 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 5, 8, 10, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.587 = CACAAACCAAATCCGT-1

using 297 reads

====================================================================================

graph has 323 edges initially, 18 edges after simplification

total ucounts = 20
nonsolo ucounts = 9[4^2, 12, 13, 19, 34, 57, 60, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.591 = CACAAACCAAGGTTTC-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.594 = CACAAACCAAGTTCTG-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.596 = CACAAACCAATCCGAT-1

using 168 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[54, 114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.601 = CACAAACCACAGACAG-1

using 248 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 10[3^2, 4, 5^3, 6^2, 12, 190]
surviving nonsolo ucounts = 1[190]
ids = [6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=376]
0-268 ==> 5722-5990 on rc of segment after TRBV28 exon 2 [len=6000] (mis=2)
273-324 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=0)
324-376 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 133, 181, 257
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.602 = CACAAACCACATGGGA-1

using 55 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.605 = CACAAACCACCATCCT-1

using 96 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 4, 5, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.607 = CACAAACCACGGATAG-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.609 = CACAAACCACGGCGTT-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.610 = CACAAACCACGGTAGA-1

using 89 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.611 = CACAAACCACTACAGT-1

using 119 reads

====================================================================================

graph has 102 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^4, 4, 5^2, 9, 14, 16, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.617 = CACAAACCAGCTGTTA-1

using 170 reads

====================================================================================

graph has 216 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 6, 37, 53, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.618 = CACAAACCAGGAATGC-1

using 42 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.621 = CACAAACCAGTTTACG-1

using 99 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[9, 12, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.623 = CACAAACCATAGACTC-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[6, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.626 = CACAAACCATCTACGA-1

using 58 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.627 = CACAAACCATCTATGG-1

using 34 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.629 = CACAAACCATGCCTAA-1

using 2709 reads

====================================================================================

graph has 866 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 4[2, 4, 6, 2689]
surviving nonsolo ucounts = 1[2689]
ids = [11]

====================================================================================

REJECT CONTIGS

TIG 1[bases=320]
5-205 ==> 5800-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
220-268 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
268-320 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 2659
start codons at 82, 92, 166
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.634 = CACAAACCATTTCACT-1

using 79 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.636 = CACAAACGTAACGTTC-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.637 = CACAAACGTACAGCAG-1

using 891 reads

====================================================================================

graph has 304 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 885]
surviving nonsolo ucounts = 1[885]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.640 = CACAAACGTACGACCC-1

using 56 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.642 = CACAAACGTAGCTAAA-1

using 108 reads

====================================================================================

graph has 96 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 11[2^5, 3^2, 15, 16, 26, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.647 = CACAAACGTCACACGC-1

using 57 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.649 = CACAAACGTCATGCCG-1

using 14280 reads

====================================================================================

graph has 4976 edges initially, 38 edges after simplification

total ucounts = 242
nonsolo ucounts = 163[2^31, 3^27, 4^12, 5^12, 6^8, 7^7, 8^4, 9^7, 10, 11^9, 12^7, 13, 14^5, 15, 16^3, 18^3, 19, 20^3, 22, 23, 24, 25, 26, 37, 52, 59, 66^2, 100, 123, 317, 634, 703, 992, 1191, 1202, 1413, 1414, 4797]
surviving nonsolo ucounts = 9[100, 634, 703, 992, 1191, 1202, 1413, 1414, 4797]
ids = [133, 122, 185, 205, 114, 188, 176, 203, 166]

====================================================================================

UMI info for barcode CACAAACGTCATGCCG-1 contig 1 = GAAGGACGTC...
umi GCAATTGTGT = 99 reads: +403 validated
umi GTCTTATCCT = 4812 reads: +403 validated
umi TATCCCCTTC = 635 reads: -363X +1 -7XX +3 -1XX +2 -4XX +2 -1XX +5 -2XX +1 -1XX +2 -1XX +7 invalidated
umi TCAACACACT = 1212 reads: +403 validated
umi TCTAACATTT = 1412 reads: +403 validated

UMI info for barcode CACAAACGTCATGCCG-1 contig 2 = GAGAAGCCTC...
umi CTTCCGTTCG = 1189 reads: +403 validated
umi GACGCCTCTC = 642 reads: +403 validated
umi TAATCTCAAT = 1424 reads: +403 validated
umi TCTATCTGGC = 988 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=533]
78-425 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
441-481 ==> 8-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
481-533 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 501 reads
cdr3 = CASSLAWGRGEGYTF at 408, score = 7 + 9
umis assigned: [133, 166, 185, 188, 203]
of which 5 are surviving nonsolos
reads assigned: 8029
start codons at 78, 102, 187, 286, 399
confident = true

TIG 2[bases=562]
0-117 ==> 0-117 on |474|TRAV19|5'UTR| [len=117] (mis=1)
117-458 ==> 0-341 on |475|TRAV19|L-REGION+V-REGION| [len=350] (mis=0)
458-520 ==> 0-62 on |442|TRAJ56|J-REGION| [len=62] (mis=1)
520-562 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 164 reads
cdr3 = CALDTGANSKLTF at 450, score = 7 + 8
umis assigned: [114, 122, 176, 205]
of which 4 are surviving nonsolos
reads assigned: 4181
start codons at 111, 117, 174, 229, 250, 340, 349
confident = true
now this is a cell
paired!

TCACAAGTCGTGGACTCAGCAGTATACTTCTGTGCTCTTGATACTGGAGCCAATAGTAAGCTGACATTTGGAAAAGGAATAACTCTGAGTGTTAGACCAG <==> GAGCAGGGGGACTCGGCCATGTATCTCTGTGCCAGCAGCTTAGCGTGGGGGCGCGGGGAAGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.654 = CACAAACGTCGAGATG-1

using 5588 reads

====================================================================================

graph has 2068 edges initially, 30 edges after simplification

total ucounts = 183
nonsolo ucounts = 118[2^36, 3^14, 4^7, 5^10, 6^6, 7^6, 8^5, 9^3, 10^3, 11^3, 12^2, 13^2, 14^2, 15, 16, 17, 18^2, 19, 20, 21, 22, 23, 25, 28, 32, 33, 73, 124, 572, 1066, 2903]
surviving nonsolo ucounts = 3[572, 1066, 2903]
ids = [161, 18, 37]

====================================================================================

UMI info for barcode CACAAACGTCGAGATG-1 contig 1 = CCGGTGTCGG...
umi ACATTAATCT = 1061 reads: +403 validated
umi CTTGGATTCT = 2 reads: -395X +1 -7XX invalidated
umi TGTGGATCCA = 573 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=621]
0-176 ==> 0-176 on |496|TRAV3|5'UTR| [len=176] (mis=0)
176-519 ==> 0-343 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=3)
525-579 ==> 6-60 on |437|TRAJ5|J-REGION| [len=60] (mis=0)
579-621 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 42 reads
cdr3 = CAVRLARAGRRALTF at 503, score = 8 + 9
umis assigned: [18, 93, 161]
of which 2 are surviving nonsolos
reads assigned: 1611
start codons at 176, 197, 206, 298, 339, 414
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.655 = CACAAACGTCGCATAT-1

using 119 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.661 = CACAAACGTCTCACCT-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.662 = CACAAACGTCTCTCGT-1

using 43 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.664 = CACAAACGTCTTGATG-1

using 55 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.668 = CACAAACGTGCTGTAT-1

using 63 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.669 = CACAAACGTGGAAAGA-1

using 59 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 23, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.671 = CACAAACGTGGTCCGT-1

using 72 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.672 = CACAAACGTGGTCTCG-1

using 81 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.675 = CACAAACGTTAGGGTG-1

using 66 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.680 = CACAAACGTTGCGTTA-1

using 35 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.685 = CACAAACGTTTGTTTC-1

using 108 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 31, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.687 = CACAAACTCAACGGCC-1

using 41 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.689 = CACAAACTCAATCACG-1

using 80 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.692 = CACAAACTCACCACCT-1

using 41 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.703 = CACAAACTCCGGCACA-1

using 46 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[4, 7^2, 10, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.704 = CACAAACTCCTACAGA-1

using 76 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.707 = CACAAACTCGCACTCT-1

using 90 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.709 = CACAAACTCGCTTAGA-1

using 42 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[4, 8^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.711 = CACAAACTCGGATGTT-1

using 2479 reads

====================================================================================

graph has 758 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 6^2, 2461]
surviving nonsolo ucounts = 1[2461]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=486]
5-27 ==> 9502-9524 on segment before TRBV21-1 exon 1 [len=9524] (mis=0)
386-434 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
434-486 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 2421
start codons at 27, 145, 176, 236, 269, 288
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.712 = CACAAACTCGGCTTGG-1

using 364 reads

====================================================================================

graph has 224 edges initially, 8 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^2, 3^3, 6, 10^2, 11, 12, 13, 27, 28, 29, 31, 71, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.714 = CACAAACTCGTACGGC-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.717 = CACAAACTCGTTACGA-1

using 67 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[19, 21, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.719 = CACAAACTCTCGCATC-1

using 37 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.721 = CACAAACTCTCTGTCG-1

using 22 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.723 = CACAAACTCTGGAGCC-1

using 45 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.724 = CACAAACTCTGGTGTA-1

using 48 reads

====================================================================================

graph has 30 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[19, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.727 = CACAAACTCTTGACGA-1

using 14 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.730 = CACAAACTCTTGTTTG-1

using 84 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 3, 11, 22, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.733 = CACACAAAGAAACCAT-1

using 47 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.739 = CACACAAAGAGCTGGT-1

using 219 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 4, 5, 13, 35, 56, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.740 = CACACAAAGATAGCAT-1

using 233 reads

====================================================================================

graph has 230 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[8^2, 9, 14, 17, 19, 46, 47, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.741 = CACACAAAGATATACG-1

using 50 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.746 = CACACAAAGATGTTAG-1

using 42 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.748 = CACACAAAGCATCATC-1

using 27 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.749 = CACACAAAGCCACCTG-1

using 110 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.750 = CACACAAAGCCAGTAG-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.751 = CACACAAAGCCCAACC-1

using 68 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.753 = CACACAAAGCGCCTTG-1

using 37 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.757 = CACACAAAGCTAGCCC-1

using 26636 reads

====================================================================================

graph has 9686 edges initially, 190 edges after simplification

total ucounts = 600
nonsolo ucounts = 401[2^78, 3^61, 4^42, 5^26, 6^21, 7^26, 8^16, 9^16, 10^12, 11^8, 12^10, 13^6, 15^6, 16^7, 17^6, 18^5, 19^5, 20^3, 21^4, 22, 23^2, 24^3, 25, 26, 28, 32^3, 35, 36, 37^2, 38^2, 39, 43^2, 44, 46, 49, 61, 63, 64, 73, 80, 92, 238, 776, 1061, 1079, 1629, 1656, 1689, 1939, 1952, 1958, 2013, 2100, 4858]
surviving nonsolo ucounts = 13[238, 776, 1061, 1079, 1629, 1656, 1689, 1939, 1952, 1958, 2013, 2100, 4858]
ids = [572, 115, 263, 445, 406, 161, 371, 253, 266, 320, ...]

====================================================================================

UMI info for barcode CACACAAAGCTAGCCC-1 contig 1 = ATTGCAGCTC...
umi GCAATAATGA = 1990 reads: +391 validated
umi GTTTGAACCA = 4859 reads: -16X +1 -1XX +373 invalidated
umi TTGTTCACGA = 2134 reads: +391 validated

UMI info for barcode CACACAAAGCTAGCCC-1 contig 2 = ACTACTGGGA...
umi AGCGGGTAAC = 2022 reads: +391 validated
umi CAACACCGCC = 1658 reads: +391 validated
umi CGTTTACATT = 1935 reads: +391 validated
umi CTCCGCAGCA = 1061 reads: +391 validated
umi CTCGCTCTCT = 1961 reads: +391 validated
umi GGCCCTGCCG = 1685 reads: +391 validated
umi GTCGCTCCTT = 1638 reads: +391 validated
umi TAGAGCACAC = 1079 reads: +391 validated
umi TTGCTTTTTT = 194 reads: -382X +1 -1XX +2 -1XX +4 invalidated

GOOD CONTIGS

TIG 1[bases=492]
0-59 ==> 8-67 on |509|TRAV4|5'UTR| [len=67] (mis=0)
59-387 ==> 0-328 on |510|TRAV4|L-REGION+V-REGION| [len=328] (mis=0)
388-450 ==> 0-62 on |424|TRAJ38|J-REGION| [len=62] (mis=0)
450-492 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 611 reads
cdr3 = CLVGDNNAGNNRKLIW at 371, score = 8 + 8
umis assigned: [320, 427, 576]
of which 3 are surviving nonsolos
reads assigned: 8782
start codons at 59, 137, 147, 198, 390
confident = true

TIG 2[bases=480]
0-37 ==> 0-37 on |606|TRBV25-1|5'UTR| [len=37] (mis=0)
37-374 ==> 0-337 on |607|TRBV25-1|L-REGION+V-REGION| [len=344] (mis=0)
377-428 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=3)
428-480 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 939 reads
cdr3 = CASTPPTGELFF at 364, score = 7 + 9
umis assigned: [119, 161, 253, 263, 266, 371, 406, 445, 572]
of which 9 are surviving nonsolos
reads assigned: 12988
start codons at 37, 61, 91, 159, 172, 179, 187, 214, 242
confident = true
now this is a cell
paired!

AGCGACACTGCTGTGTACTACTGCCTCGTGGGTGACAATAATGCTGGCAACAACCGTAAGCTGATTTGGGGATTGGGAACAAGCCTGGCAGTAAATCCGA <==> GAGTCTGCCAGGCCCTCACATACCTCTCAGTACCTCTGTGCCAGCACCCCCCCCACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.760 = CACACAAAGGACAGCT-1

using 100 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[4, 6, 7^2, 14, 20, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.763 = CACACAAAGGGCTCTC-1

using 106 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.767 = CACACAAAGGTGGGTT-1

using 103 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 5, 8, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.769 = CACACAAAGTATCGAA-1

using 79 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.773 = CACACAAAGTCGAGTG-1

using 46 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 14, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.774 = CACACAAAGTGACATA-1

using 54 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3, 4, 5, 11, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.775 = CACACAAAGTTCGCGC-1

using 120 reads

====================================================================================

graph has 98 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2, 3^3, 4^2, 5, 12, 25^2, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.779 = CACACAAAGTTTCCTT-1

using 26 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.780 = CACACAACAAACAACA-1

using 65 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.784 = CACACAACAACGATCT-1

using 102 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[25, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.785 = CACACAACAAGACGTG-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.789 = CACACAACAAGTACCT-1

using 47 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.790 = CACACAACAATAGAGT-1

using 113 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.793 = CACACAACACAGACTT-1

using 58 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.795 = CACACAACACATTAGC-1

using 38 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.799 = CACACAACACGCCAGT-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.801 = CACACAACACGTCAGC-1

using 178 reads

====================================================================================

graph has 126 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 4, 5, 10, 14^2, 18, 24, 35, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.804 = CACACAACAGACACTT-1

using 114 reads

====================================================================================

graph has 184 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3^2, 4^2, 10, 23, 64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.807 = CACACAACAGCCAGAA-1

using 9381 reads

====================================================================================

graph has 1582 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2, 3, 4^2, 9360]
surviving nonsolo ucounts = 1[9360]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.812 = CACACAACAGGGATTG-1

using 63 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.814 = CACACAACAGGTGGAT-1

using 44 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^2, 3^2, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.818 = CACACAACATAACCTG-1

using 73 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.821 = CACACAACATAGTAAG-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.822 = CACACAACATCCTAGA-1

using 44 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.827 = CACACAACATTGAGCT-1

using 17 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.830 = CACACAAGTACCATCA-1

using 138 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2, 4, 10^2, 13, 14, 15, 27, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.834 = CACACAAGTAGCCTAT-1

using 64 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.835 = CACACAAGTAGCGATG-1

using 92 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 15[2^6, 4^3, 8^2, 9, 10, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.837 = CACACAAGTCAATACC-1

using 81 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.840 = CACACAAGTCATACTG-1

using 93 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.845 = CACACAAGTCGTCTTC-1

using 60 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.846 = CACACAAGTCTAACGT-1

using 49 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^3, 3, 5, 15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.848 = CACACAAGTCTGATTG-1

using 72 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 5, 6, 11, 14^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.849 = CACACAAGTGACAAAT-1

using 85 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 5, 6, 7, 11, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.852 = CACACAAGTGCCTGGT-1

using 92 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.855 = CACACAAGTGTCCTCT-1

using 147 reads

====================================================================================

graph has 166 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2^3, 4^3, 6^2, 8, 17, 20, 22, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.858 = CACACAAGTTCAGGCC-1

using 37 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.859 = CACACAAGTTCAGTAC-1

using 16 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.861 = CACACAAGTTCTGAAC-1

using 79 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.863 = CACACAATCAACGCTA-1

using 42 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.864 = CACACAATCAACGGCC-1

using 100 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.866 = CACACAATCACAACGT-1

using 70 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 7, 8, 10, 11, 15, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.867 = CACACAATCACCACCT-1

using 32 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.868 = CACACAATCACGGTTA-1

using 51 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.870 = CACACAATCAGTACGT-1

using 147 reads

====================================================================================

graph has 144 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2, 3, 5^2, 7, 8, 9, 11, 13, 14^2, 16, 17, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.875 = CACACAATCATGGTCA-1

using 51 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.876 = CACACAATCATGTCCC-1

using 46 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.877 = CACACAATCCACGAAT-1

using 44 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.878 = CACACAATCCAGATCA-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.882 = CACACAATCCTCTAGC-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.886 = CACACAATCGGAATCT-1

using 155 reads

====================================================================================

graph has 97 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[3^4, 4^2, 5, 9, 54, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.887 = CACACAATCGGCGGTT-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.891 = CACACAATCTAACTTC-1

using 138 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[4, 6, 7, 10, 12, 43, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.892 = CACACAATCTATCCTA-1

using 149 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2^2, 3, 5^2, 8^2, 9^2, 15, 20, 25, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.893 = CACACAATCTCCGGTT-1

using 65 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.897 = CACACAATCTCTTATG-1

using 31 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.899 = CACACAATCTGATACG-1

using 40 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.901 = CACACAATCTGTTGAG-1

using 62 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.903 = CACACCTAGAAACCTA-1

using 39 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 5, 6, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.906 = CACACCTAGAATTCCC-1

using 35 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.907 = CACACCTAGACAATAC-1

using 42 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.909 = CACACCTAGAGCTATA-1

using 35926 reads

====================================================================================

graph has 14889 edges initially, 414 edges after simplification

total ucounts = 1177
nonsolo ucounts = 922[2^135, 3^87, 4^77, 5^58, 6^50, 7^34, 8^50, 9^37, 10^20, 11^24, 12^34, 13^18, 14^21, 15^17, 16^17, 17^26, 18^19, 19^13, 20^13, 21^16, 22^12, 23^7, 24^9, 25^6, 26^11, 27^8, 28^8, 29^4, 30^5, 31^9, 32^4, 33^2, 34^3, 35^4, 36, 37^5, 38^4, 39^3, 40^2, 41, 42, 43, 44^3, 45^3, 46, 47, 48^2, 49, 50^2, 51^2, 53, 54, 55, 59, 60, 64^2, 67, 69, 74^3, 77, 84, 92, 102, 129, 172, 401, 653, 873, 1143, 1168, 1313, 1391, 1448, 1890, 2143, 3416, 4178, 4376]
surviving nonsolo ucounts = 14[172, 401, 653, 873, 1143, 1168, 1313, 1391, 1448, 1890, 2143, 3416, 4178, 4376]
ids = [901, 868, 1141, 918, 886, 27, 0, 1123, 665, 471, ...]

====================================================================================

UMI info for barcode CACACCTAGAGCTATA-1 contig 1 = CCGGTGTCGG...
umi AACCATTGGG = 1169 reads: +400 validated
umi ATTGGATCCT = 4189 reads: -231X +169 invalidated
umi GTGGTCTGCC = 2116 reads: -366 +5 -2XX +1 -1XX +5 -1XX +3 -1XX +1 -4XX +2 -3XX +2 -1XX +1 -1XX invalidated
umi TACATTCTCT = 405 reads: +400 validated
umi TCAATCCTCA = 854 reads: +361 -5 +34 non-validated
umi TTGTTAGCCA = 656 reads: +378 -1 +21 non-validated

UMI info for barcode CACACCTAGAGCTATA-1 contig 2 = CATGTTCACA...
umi CCTATAGGGA = 1882 reads: +397 validated
umi GTCTTTTTGG = 2134 reads: +397 validated
umi TAGATCCCTT = 1150 reads: +397 validated
umi TATCTGACCA = 133 reads: -377X +1 -3X +3 -5X +1 -5XX +2 invalidated
umi TTGCAACCCC = 1391 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=618]
0-176 ==> 0-176 on |496|TRAV3|5'UTR| [len=176] (mis=0)
176-519 ==> 0-343 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=2)
513-576 ==> 0-63 on |431|TRAJ44|J-REGION| [len=63] (mis=4)
576-618 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 281 reads
cdr3 = CAVRGTGTASKLTF at 503, score = 8 + 9
umis assigned: [27, 270, 835, 868, 918, 1141]
of which 6 are surviving nonsolos
reads assigned: 9263
start codons at 176, 197, 206, 298, 339, 414
confident = true

TIG 2[bases=502]
0-53 ==> 6-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=0)
53-400 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=4)
392-404 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
403-450 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 4 umis using 350 reads
cdr3 = CASRTGGSYEQYF at 383, score = 8 + 8
umis assigned: [471, 829, 886, 901, 1123]
of which 5 are surviving nonsolos
reads assigned: 6588
start codons at 1, 53, 111, 152, 175, 224, 227, 276, 287, 314, 321
confident = true

REJECT CONTIGS

TIG 1[bases=387]
0-276 ==> 5724-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
287-335 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=2)
335-387 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [0, 78, 665]
of which 3 are surviving nonsolos
reads assigned: 6017
start codons at 153, 163, 237
confident = false
did not find CDR3
now this is a cell
paired!

CTTGTGAGCGACTCCGCTTTGTACTTCTGTGCTGTGAGAGGGACCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> CCCTCAGAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGGACAGGGGGTTCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.910 = CACACCTAGAGCTGCA-1

using 945 reads

====================================================================================

graph has 382 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[7^2, 9, 23, 26, 873]
surviving nonsolo ucounts = 1[873]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.911 = CACACCTAGAGGGATA-1

using 96 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3, 8^2, 14, 16, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.912 = CACACCTAGAGGGCTT-1

using 306 reads

====================================================================================

graph has 130 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 4^2, 8, 29, 31, 51, 69, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.914 = CACACCTAGATACACA-1

using 32 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.915 = CACACCTAGATAGTCA-1

using 33 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.917 = CACACCTAGATGCCTT-1

using 135 reads

====================================================================================

graph has 186 edges initially, 2 edges after simplification

total ucounts = 30
nonsolo ucounts = 20[2^9, 3^2, 4^3, 5, 8, 14^2, 24^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.926 = CACACCTAGCTAACTC-1

using 61 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 3^2, 5, 7, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.929 = CACACCTAGCTGCAAG-1

using 155 reads

====================================================================================

graph has 210 edges initially, 14 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^2, 3, 4, 5, 6, 8^2, 11, 15, 25, 26, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.932 = CACACCTAGGATGGTC-1

using 67 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 4^2, 5, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.933 = CACACCTAGGATGTAT-1

using 55 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.934 = CACACCTAGGCACATG-1

using 18 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.935 = CACACCTAGGCGATAC-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.938 = CACACCTAGGGTGTGT-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.942 = CACACCTAGGTGCTTT-1

using 124 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2^2, 3, 5, 6, 7, 13, 17, 25, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.943 = CACACCTAGTAACCCT-1

using 791 reads

====================================================================================

graph has 598 edges initially, 18 edges after simplification

total ucounts = 31
nonsolo ucounts = 22[2, 3, 5, 6, 8, 11, 12, 18, 22, 29, 32, 33, 39, 46, 54^2, 57, 58, 63, 72, 76, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.948 = CACACCTAGTGACTCT-1

using 453 reads

====================================================================================

graph has 210 edges initially, 8 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[3^2, 4, 5, 7, 8, 10^2, 15, 17, 22, 32, 55, 61, 69, 128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.950 = CACACCTAGTTATCGC-1

using 51 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 3, 4, 5, 6, 7, 8, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.952 = CACACCTAGTTGTCGT-1

using 68 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.953 = CACACCTCAAACAACA-1

using 38 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.954 = CACACCTCAAACTGCT-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.955 = CACACCTCAAAGCAAT-1

using 65 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 6, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.957 = CACACCTCAACACGCC-1

using 66 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.958 = CACACCTCAACTTGAC-1

using 260 reads

====================================================================================

graph has 252 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 8, 9, 12^2, 15, 20, 27, 69, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.965 = CACACCTCAAGTCTAC-1

using 122 reads

====================================================================================

graph has 36 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[48, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.966 = CACACCTCAAGTTGTC-1

using 63 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.970 = CACACCTCACAACGCC-1

using 45 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 4[7^2, 9, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.976 = CACACCTCACATAACC-1

using 40 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.980 = CACACCTCACATTTCT-1

using 63 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.983 = CACACCTCACCAGTTA-1

using 61 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 12, 14, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.987 = CACACCTCACGAAAGC-1

using 4321 reads

====================================================================================

graph has 2479 edges initially, 103 edges after simplification

total ucounts = 358
nonsolo ucounts = 216[2^54, 3^30, 4^15, 5^14, 6^12, 7^12, 8^11, 9^6, 10^9, 11^5, 12^2, 13, 14^3, 15^5, 16^3, 17^4, 18^4, 19^3, 20^3, 21^4, 22, 24^2, 29, 32, 35^2, 37, 40, 53, 56, 66, 68, 144, 1080, 1120]
surviving nonsolo ucounts = 3[144, 1080, 1120]
ids = [352, 297, 42]

====================================================================================

UMI info for barcode CACACCTCACGAAAGC-1 contig 1 = GGGGGCTGTT...
umi AGGACACACG = 1124 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=490]
61-403 ==> 0-342 on |733|TRBV11-2|L-REGION+V-REGION| [len=342] (mis=1)
432-473 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
junction support: 1 umis using 61 reads
cdr3 = CASSLGSTNRGFMGEQFF at 391, score = 8 + 8
umis assigned: [42]
of which 1 are surviving nonsolos
reads assigned: 1104
start codons at 61, 203, 284, 427
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.991 = CACACCTCACTTCGAA-1

using 104 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.993 = CACACCTCAGACACTT-1

using 76 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 7^2, 9, 10, 11, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.999 = CACACCTCAGATTGCT-1

using 99 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.1000 = CACACCTCAGCATGAG-1

using 57 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 3^2, 4^2, 8, 15, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.1004 = CACACCTCAGCGTTCG-1

using 60 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 9^2, 13, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.1005 = CACACCTCAGCTCCGA-1

using 41 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 21.1006 = CACACCTCAGCTCGCA-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.17
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk21-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk21-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

90.171 seconds used processing barcodes, peak mem = 0.35
