[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 832.79 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk15-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk15-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk15.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.0 = AGGGATGGTAAACACA-1

using 48847 reads

====================================================================================

graph has 26863 edges initially, 872 edges after simplification

total ucounts = 2309
nonsolo ucounts = 1795[2^254, 3^157, 4^118, 5^89, 6^79, 7^86, 8^64, 9^57, 10^57, 11^51, 12^61, 13^48, 14^39, 15^38, 16^40, 17^46, 18^35, 19^38, 20^33, 21^28, 22^29, 23^20, 24^22, 25^19, 26^20, 27^27, 28^21, 29^8, 30^13, 31^18, 32^16, 33^11, 34^10, 35^6, 36^4, 37^7, 38^9, 39^11, 40^2, 41^9, 42^5, 43^5, 44^5, 45^5, 46^6, 47^2, 48^7, 49^4, 50^3, 51^3, 52^3, 53^3, 54, 55^3, 56^2, 57^6, 58^3, 59^3, 61^3, 62^3, 66, 67^3, 69, 74, 75^2, 76^2, 79, 80, 81, 89, 92, 99, 110, 3313, 9146, 10961]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=316]
7-208 ==> 481-682 on segment before TRAJ32 exon 1 [len=682] (mis=0)
208-274 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=0)
274-316 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [241, 513, 1166, 1507]
of which 0 are surviving nonsolos
reads assigned: 21799
start codons at 24, 35, 65, 80, 214
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.3 = AGGGATGGTACAGACG-1

using 53 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.4 = AGGGATGGTACGCACC-1

using 46 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.9 = AGGGATGGTAGCGTGA-1

using 13473 reads

====================================================================================

graph has 5527 edges initially, 110 edges after simplification

total ucounts = 569
nonsolo ucounts = 416[2^75, 3^42, 4^43, 5^37, 6^27, 7^17, 8^12, 9^16, 10^8, 11^14, 12^9, 13^7, 14^8, 15^3, 16^7, 17^4, 18^7, 19, 20^4, 21^5, 22^4, 23, 24^2, 25, 26^3, 27^3, 28^4, 29, 30^2, 31^2, 32^2, 33, 34, 35, 36^3, 37^2, 38, 40, 41^3, 42, 43^2, 44, 45, 47, 49, 50, 52^2, 54^3, 56, 57, 58^2, 59^2, 64, 67, 71, 72^2, 75^2, 79^2, 87, 88, 110, 7996]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.13 = AGGGATGGTATTAGCC-1

using 32 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.14 = AGGGATGGTCACTTCC-1

using 127 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3^2, 10, 14, 46, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.17 = AGGGATGGTCCCTACT-1

using 44 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.19 = AGGGATGGTCCTCTTG-1

using 61 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.26 = AGGGATGGTCTTGTCC-1

using 74 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[3, 9, 10, 15, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.32 = AGGGATGGTGATAAAC-1

using 48 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 4, 6, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.33 = AGGGATGGTGATAAGT-1

using 206 reads

====================================================================================

graph has 140 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[13, 15, 16^2, 25, 35, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.35 = AGGGATGGTGCACCAC-1

using 54 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 4, 5, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.40 = AGGGATGGTGGTTTCA-1

using 44 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.42 = AGGGATGGTGTAAGTA-1

using 21 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.44 = AGGGATGGTGTGGTTT-1

using 27120 reads

====================================================================================

graph has 12541 edges initially, 288 edges after simplification

total ucounts = 1031
nonsolo ucounts = 772[2^103, 3^79, 4^48, 5^55, 6^30, 7^32, 8^51, 9^25, 10^23, 11^19, 12^18, 13^31, 14^20, 15^21, 16^17, 17^19, 18^13, 19^10, 20^12, 21^7, 22^8, 23^7, 24^8, 25^8, 26^4, 27^5, 28^7, 29^2, 30, 31^3, 32^2, 33^5, 34^3, 35^8, 36^2, 37^6, 38^3, 39^3, 40, 42^3, 43^2, 44^2, 45, 46, 47^3, 48, 53^3, 54^2, 55, 57^3, 58^2, 59^2, 60, 61, 62, 63, 64, 66^2, 69^2, 75, 79, 80, 89, 91, 94, 101, 117, 300, 305, 686, 803, 930, 934, 1333, 1638, 1764, 8028]
surviving nonsolo ucounts = 16[2, 3, 5, 6, 9, 91, 300, 305, 686, 803, 930, 934, 1333, 1638, 1764, 8028]
ids = [896, 939, 897, 631, 169, 401, 463, 18, 788, 6, ...]

====================================================================================

UMI info for barcode AGGGATGGTGTGGTTT-1 contig 1 = ACTCACTGGG...
umi AACGTCATCC = 275 reads: -373 +27 non-validated
umi CTGCAACCGT = 1333 reads: +400 validated
umi GGAAACCGTA = 1776 reads: +400 validated
umi TAGCATATCT = 949 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=521]
69-412 ==> 0-343 on |639|TRBV5-5|L-REGION+V-REGION| [len=343] (mis=1)
422-469 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
469-521 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 254 reads
cdr3 = CASSLVGGGRYEQYF at 396, score = 8 + 8
umis assigned: [18, 470, 609, 765]
of which 4 are surviving nonsolos
reads assigned: 4257
start codons at 69, 191, 274, 355
confident = true

REJECT CONTIGS

TIG 1[bases=464]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=3)
360-422 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=3)
422-464 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CSNSPYSSASKIIF at 349, score = 3 + 9
umis assigned: [169, 747]
of which 2 are surviving nonsolos
reads assigned: 1609
start codons at 23, 78, 87, 237
confident = false
frameshifted full length stopped transcript of length 464
VJ delta = 22
not full
not full

TIG 2[bases=317]
7-64 ==> 47-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
64-113 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
113-215 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
265-317 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [94, 778]
of which 2 are surviving nonsolos
reads assigned: 8552
start codons at 
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.45 = AGGGATGGTGTTTGGT-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.48 = AGGGATGGTTATCACG-1

using 44 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.49 = AGGGATGGTTATCGGT-1

using 14 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.57 = AGGGATGGTTGGTAAA-1

using 106 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.59 = AGGGATGGTTGTCTTT-1

using 62 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.62 = AGGGATGGTTTAGGAA-1

using 64 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.65 = AGGGATGTCAACGGCC-1

using 3103 reads

====================================================================================

graph has 2886 edges initially, 76 edges after simplification

total ucounts = 458
nonsolo ucounts = 310[2^66, 3^36, 4^36, 5^26, 6^29, 7^11, 8^12, 9^9, 10^4, 11^3, 12^7, 13^7, 14^7, 15^5, 16^2, 17^11, 18^3, 19^3, 20^2, 22, 23^3, 24, 25, 26^3, 27, 28^3, 30, 36, 37^2, 39, 42^3, 43, 45^2, 49, 51, 60, 62^2, 83, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.69 = AGGGATGTCACGGTTA-1

using 54 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.74 = AGGGATGTCAGCATGT-1

using 96 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 10, 13, 16, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.75 = AGGGATGTCAGGCAAG-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.76 = AGGGATGTCAGGCCCA-1

using 3541 reads

====================================================================================

graph has 3571 edges initially, 70 edges after simplification

total ucounts = 485
nonsolo ucounts = 320[2^61, 3^42, 4^29, 5^28, 6^14, 7^16, 8^23, 9^12, 10^8, 11^12, 12^8, 13^5, 14^5, 15^4, 16^5, 17^7, 18^2, 19^3, 20, 21^2, 22^5, 23^2, 28, 30^2, 35, 36, 38^4, 40, 42^2, 44, 45^2, 50, 56, 59, 62, 66, 67, 74, 82, 87, 94, 105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.77 = AGGGATGTCAGGCGAA-1

using 62 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 26, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.84 = AGGGATGTCATCTGTT-1

using 44 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.85 = AGGGATGTCATGCTCC-1

using 89 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.92 = AGGGATGTCCCTAATT-1

using 9602 reads

====================================================================================

graph has 5578 edges initially, 126 edges after simplification

total ucounts = 515
nonsolo ucounts = 369[2^63, 3^40, 4^31, 5^21, 6^14, 7^17, 8^21, 9^15, 10^10, 11^10, 12^4, 13^7, 14^11, 15^12, 16^5, 17^4, 18^5, 19^6, 20^6, 21^3, 22^3, 23^10, 24, 25^4, 26^3, 27^3, 29, 31^4, 32^2, 35^2, 38, 39, 40, 43^2, 44, 45^2, 46, 48, 50, 52, 53, 57, 61^2, 62, 67, 69, 75, 84, 85, 95, 98, 157, 166, 240, 718, 975, 1033, 1701]
surviving nonsolo ucounts = 7[157, 166, 240, 718, 975, 1033, 1701]
ids = [244, 99, 382, 245, 134, 296, 303]

====================================================================================

UMI info for barcode AGGGATGTCCCTAATT-1 contig 1 = AGATCTCCCA...
umi ATTGCTCCTC = 167 reads: +358 -17 +7 non-validated
umi CAGCCTCGAC = 961 reads: -266 +1 -1XX +114 invalidated
umi CTATTGATCA = 159 reads: +358 -1 +4 -2 +6 -1 +10 non-validated
umi CTATTGATCG = 719 reads: +382 validated
umi GCCCATCCAC = 1711 reads: +382 validated
umi TCTCAGGACT = 1 reads: -382X invalidated

GOOD CONTIGS

TIG 1[bases=528]
0-94 ==> 18-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
94-429 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=5)
423-435 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
435-476 ==> 6-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
476-528 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 164 reads
cdr3 = CAWGQGGEQYF at 415, score = 7 + 8
umis assigned: [99, 134, 244, 245, 303, 419]
of which 5 are surviving nonsolos
reads assigned: 3626
start codons at 91, 94, 162
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.96 = AGGGATGTCCTCATTA-1

using 34 reads

====================================================================================

graph has 24 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[13, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.97 = AGGGATGTCCTCGCAT-1

using 58 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.101 = AGGGATGTCCTTCAAT-1

using 351 reads

====================================================================================

graph has 282 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^2, 6^3, 11, 16, 26, 35, 40, 54, 69, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.102 = AGGGATGTCCTTTACA-1

using 982 reads

====================================================================================

graph has 384 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 3, 9, 962]
surviving nonsolo ucounts = 1[962]
ids = [6]

====================================================================================

UMI info for barcode AGGGATGTCCTTTACA-1 contig 1 = TGGGGAGTAA...
umi TCAACGGGTC = 979 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=612]
179-515 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=4)
509-570 ==> 0-61 on |427|TRAJ40|J-REGION| [len=61] (mis=4)
570-612 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CAVASGTYKYIF at 503, score = 6 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 960
start codons at 18, 50, 84, 179, 249, 386, 411
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.108 = AGGGATGTCGGAATCT-1

using 81 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 8, 11, 12, 14, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.109 = AGGGATGTCGGAGGTA-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.110 = AGGGATGTCGGGAGTA-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.111 = AGGGATGTCGGTCTAA-1

using 34 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.117 = AGGGATGTCTAACGGT-1

using 140 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3^2, 4, 126]
surviving nonsolo ucounts = 1[126]
ids = [3]

====================================================================================

UMI info for barcode AGGGATGTCTAACGGT-1 contig 1 = GAACAATGAT...
umi GCATAGACGC = 87 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=405]
8-348 ==> 0-340 on |466|TRAV13-2|L-REGION+V-REGION| [len=340] (mis=0)
347-405 ==> 6-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CAENTGGGNKLTF at 335, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 83
start codons at 5, 8, 32, 227
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.119 = AGGGATGTCTACTATC-1

using 53 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.121 = AGGGATGTCTATGTGG-1

using 122 reads

====================================================================================

graph has 78 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 3, 5, 14^2, 22, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.126 = AGGGATGTCTCGCTTG-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.132 = AGGGATGTCTGGCGTG-1

using 40 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.134 = AGGGATGTCTGGTATG-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.137 = AGGGATGTCTTACCGC-1

using 85 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 3, 19, 21, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.141 = AGGGATGTCTTGAGAC-1

using 42590 reads

====================================================================================

graph has 20955 edges initially, 620 edges after simplification

total ucounts = 1823
nonsolo ucounts = 1458[2^182, 3^128, 4^95, 5^71, 6^66, 7^54, 8^42, 9^36, 10^38, 11^36, 12^25, 13^25, 14^34, 15^26, 16^15, 17^23, 18^24, 19^14, 20^15, 21^11, 22^10, 23^15, 24^14, 25^15, 26^14, 27^10, 28^9, 29^15, 30^13, 31^8, 32^13, 33^10, 34^11, 35^11, 36^7, 37^6, 38^9, 39^13, 40^11, 41^10, 42^11, 43^8, 44^12, 45^6, 46^8, 47^8, 48^4, 49^12, 50^8, 51^13, 52^7, 53^4, 54^10, 55^9, 56^5, 57^6, 58^3, 59^6, 60^7, 61^11, 62^4, 63^7, 64^5, 65^2, 66^9, 67^2, 68^3, 69^4, 70^3, 71^4, 72^5, 74^4, 75^3, 76^3, 77^2, 78^2, 79^4, 80^3, 81, 82^2, 84, 85^4, 86^2, 88, 89^2, 90^3, 91^2, 93^2, 94, 96^2, 97^2, 98^2, 101^2, 103, 107, 108^2, 109, 110, 113^2, 115, 116, 118, 122, 124, 130, 133, 1104, 3667, 6286]
surviving nonsolo ucounts = 3[1104, 3667, 6286]
ids = [235, 1392, 1690]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.143 = AGGGATGTCTTGTCAT-1

using 8 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.144 = AGGGTGAAGAAGGTGA-1

using 64 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 7, 12, 19, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.148 = AGGGTGAAGAGTGAGA-1

using 32 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.149 = AGGGTGAAGATAGGAG-1

using 57 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[16, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.150 = AGGGTGAAGATGCGAC-1

using 77 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3^2, 13, 21, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.151 = AGGGTGAAGATGTAAC-1

using 75 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 7, 24, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.152 = AGGGTGAAGCCAGAAC-1

using 59 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[6, 7, 18, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.153 = AGGGTGAAGCCGATTT-1

using 88 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[4, 7, 11, 29, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.156 = AGGGTGAAGCTGCAAG-1

using 135 reads

====================================================================================

graph has 146 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 14[2^3, 3^3, 4, 5, 7, 8, 12, 18, 23, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.164 = AGGGTGAAGGTGATAT-1

using 48 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 9, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.167 = AGGGTGAAGTAGCCGA-1

using 87 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.171 = AGGGTGAAGTTATCGC-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.173 = AGGGTGACAAAGGAAG-1

using 62 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 11, 19, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.175 = AGGGTGACAAAGTGCG-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.180 = AGGGTGACAAGGGTCA-1

using 1109 reads

====================================================================================

graph has 484 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 38, 1065]
surviving nonsolo ucounts = 1[1065]
ids = [0]

====================================================================================

UMI info for barcode AGGGTGACAAGGGTCA-1 contig 1 = AAGATCCTGA...
umi AAGCAGCCTG = 1069 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=580]
0-147 ==> 62-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
147-475 ==> 0-328 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=3)
475-538 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=0)
538-580 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CIVRLITQGGSEKLVF at 459, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1044
start codons at 20, 147, 199, 225, 286, 292, 343, 358, 367, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.186 = AGGGTGACACCAACCG-1

using 121 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.189 = AGGGTGACACCATCCT-1

using 13844 reads

====================================================================================

graph has 9851 edges initially, 280 edges after simplification

total ucounts = 2526
nonsolo ucounts = 1510[2^433, 3^211, 4^125, 5^93, 6^94, 7^80, 8^56, 9^39, 10^43, 11^32, 12^25, 13^24, 14^21, 15^24, 16^16, 17^21, 18^12, 19^13, 20^10, 21^11, 22^8, 23^9, 24^10, 25^2, 26^8, 27^7, 28^2, 29^4, 30^6, 31^4, 32^6, 33, 34, 35, 36^5, 37^6, 38, 39, 40, 41^2, 42^3, 43, 44, 45^4, 46, 47^2, 49^2, 50, 51^3, 52, 53^2, 55^2, 57, 58^2, 59, 60, 62^2, 64, 66^2, 68, 72, 73, 75, 76, 83, 84, 92, 108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.194 = AGGGTGACACTTACGA-1

using 83 reads

====================================================================================

graph has 155 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3, 15, 26, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.195 = AGGGTGACAGAAGCAC-1

using 47 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.201 = AGGGTGACAGCCAGAA-1

using 103 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.202 = AGGGTGACAGCTCGCA-1

using 11505 reads

====================================================================================

graph has 6487 edges initially, 134 edges after simplification

total ucounts = 820
nonsolo ucounts = 557[2^107, 3^66, 4^49, 5^35, 6^32, 7^29, 8^28, 9^18, 10^17, 11^8, 12^11, 13^14, 14^6, 15^10, 16^10, 17^8, 18^13, 19^9, 20^4, 21^2, 22^6, 23^3, 24^3, 25^3, 26, 27^3, 28^5, 29^2, 30, 31^5, 32^2, 34^2, 35, 36^3, 37^2, 39, 40^3, 41^4, 42, 43^2, 45, 46^2, 48^3, 50, 51, 52^2, 53^2, 54^2, 55^2, 57, 65^2, 70, 73, 82, 83, 117, 254, 311, 553, 3870]
surviving nonsolo ucounts = 4[254, 311, 553, 3870]
ids = [794, 796, 243, 624]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.203 = AGGGTGACAGCTGTAT-1

using 88 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.214 = AGGGTGACATCACGAT-1

using 28 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.218 = AGGGTGACATCTATGG-1

using 44 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[4, 11, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.219 = AGGGTGACATGTTCCC-1

using 36 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 13, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.221 = AGGGTGACATTCTCAT-1

using 42 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 6, 7, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.223 = AGGGTGAGTAAGTAGT-1

using 102 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[3, 12, 13, 33, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.225 = AGGGTGAGTACCGCTG-1

using 3123 reads

====================================================================================

graph has 2228 edges initially, 66 edges after simplification

total ucounts = 393
nonsolo ucounts = 227[2^52, 3^25, 4^17, 5^16, 6^15, 7^8, 8^13, 9^5, 10^4, 11^4, 12^3, 13^6, 15^4, 16^2, 17, 18^3, 20^2, 21, 22^2, 23, 24^4, 26, 27^4, 29, 30^5, 32, 33, 34, 35, 36^2, 37^3, 39, 41, 42^2, 43, 44, 46, 47^2, 48, 51, 54^2, 58^2, 67, 81, 100, 158]
surviving nonsolo ucounts = 2[27, 158]
ids = [356, 260]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.227 = AGGGTGAGTACGCTGC-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.236 = AGGGTGAGTATATGAG-1

using 885 reads

====================================================================================

graph has 587 edges initially, 30 edges after simplification

total ucounts = 53
nonsolo ucounts = 36[2^4, 3^4, 4, 5, 6, 7, 8^4, 9, 11^2, 12, 17, 18, 20, 26, 30, 37, 38, 43, 45, 48, 49, 56, 66, 67, 74, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.240 = AGGGTGAGTCGGGTCT-1

using 55 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.241 = AGGGTGAGTCTAAACC-1

using 115 reads

====================================================================================

graph has 162 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2^2, 3^2, 5, 8^2, 9, 16, 20, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.243 = AGGGTGAGTGCAACTT-1

using 57 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[6, 8, 9, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.245 = AGGGTGAGTGCGATAG-1

using 111 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.247 = AGGGTGAGTGGGTATG-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.248 = AGGGTGAGTGGGTCAA-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.249 = AGGGTGAGTGTGCGTC-1

using 18023 reads

====================================================================================

graph has 3114 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 8[2^2, 3, 5, 22, 72, 1701, 16208]
surviving nonsolo ucounts = 2[1701, 16208]
ids = [11, 6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.255 = AGGGTGAGTTCAGACT-1

using 55 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 7, 17, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.256 = AGGGTGAGTTCAGGCC-1

using 51 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[8, 16, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.257 = AGGGTGAGTTCATGGT-1

using 30 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.258 = AGGGTGAGTTCCACTC-1

using 22 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.260 = AGGGTGAGTTCCTCCA-1

using 62 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.264 = AGGGTGAGTTGTACAC-1

using 207 reads

====================================================================================

graph has 147 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^3, 3, 5^2, 6, 8, 31, 69, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.266 = AGGGTGAGTTTCGCTC-1

using 58 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3^2, 8, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.267 = AGGGTGAGTTTGTTGG-1

using 37 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.271 = AGGGTGATCACATACG-1

using 20787 reads

====================================================================================

graph has 12473 edges initially, 322 edges after simplification

total ucounts = 1151
nonsolo ucounts = 840[2^126, 3^68, 4^54, 5^53, 6^44, 7^34, 8^38, 9^39, 10^35, 11^24, 12^28, 13^23, 14^25, 15^15, 16^9, 17^9, 18^16, 19^8, 20^15, 21^15, 22^6, 23^8, 24^7, 25^10, 26^9, 27^4, 28^6, 29^5, 30^7, 31^6, 32^9, 33^9, 34^4, 35^3, 36^3, 37^3, 38^4, 39^6, 40^2, 41^2, 42, 43^3, 44^3, 45^5, 46^2, 47^2, 48^2, 49^2, 50, 51^2, 53^2, 54, 55, 57, 58^2, 60^2, 62, 67, 69^2, 70^2, 81, 89, 109, 278, 385, 397, 1150, 1177, 1217, 1895, 3075]
surviving nonsolo ucounts = 8[278, 385, 397, 1150, 1177, 1217, 1895, 3075]
ids = [617, 1011, 296, 486, 934, 924, 380, 920]

====================================================================================

UMI info for barcode AGGGTGATCACATACG-1 contig 1 = GAGAGTCCTG...
umi CATTCCGCAT = 1896 reads: +394 validated
umi CTGTTTCAGT = 2 reads: -390 +1 -3XX invalidated
umi GAACTCTGCC = 7 reads: +42 -1 +12 -1 +5 -1 +1 -1 +1 -1 +16 -1 +3 -1 +3 -68 +3 -2 +4 -1 +2 -1 +1 -2 +7 -1 +4 -1 +5 -1X +36 -1 +4 -1 +14 -145 invalidated
umi TCACTCCGGA = 3080 reads: +394 validated
umi TCAGCTCGGC = 1127 reads: -358 +1 -15XX +1 -3XX +3 -5XX +1 -5XX +2 invalidated
umi TGACTTCCGT = 393 reads: +394 validated

UMI info for barcode AGGGTGATCACATACG-1 contig 2 = TTTTGAAACC...
umi CAACACCCAT = 392 reads: +351 -27 +19 non-validated
umi CGCATATCTG = 1065 reads: +397 validated
umi GTCTGCTGCT = 1 reads: -384X +1 -3X +2 -7X invalidated
umi TCCAGTGGGT = 1100 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=512]
0-66 ==> 0-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
66-402 ==> 0-336 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=0)
413-460 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
460-512 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 324 reads
cdr3 = CASRLADAYEQYF at 393, score = 9 + 8
umis assigned: [380, 592, 617, 920, 924, 1011]
of which 5 are surviving nonsolos
reads assigned: 6391
start codons at 24, 52, 66, 124, 177, 201, 208, 216, 243, 307, 316
confident = true

TIG 2[bases=567]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=1)
465-525 ==> 9-69 on |439|TRAJ52|J-REGION| [len=69] (mis=0)
525-567 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 32 reads
cdr3 = CAVSGTSYGKLTF at 455, score = 7 + 9
umis assigned: [296, 486, 796, 934]
of which 3 are surviving nonsolos
reads assigned: 2513
start codons at 128, 291, 431, 477
confident = true
now this is a cell
paired!

GCCCATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGAGTGGTACTAGCTATGGAAAGCTGACATTTGGACAAGGGACCATCTTGACTGTCCATCCAA <==> TCGGCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCCGACTAGCGGACGCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.277 = AGGGTGATCAGTCCCT-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.278 = AGGGTGATCATGCTCC-1

using 89 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.280 = AGGGTGATCATTGCGA-1

using 89 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 4, 9, 34, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.284 = AGGGTGATCCACGCAG-1

using 165 reads

====================================================================================

graph has 133 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3, 4, 6, 16, 46, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.290 = AGGGTGATCCTTTACA-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.294 = AGGGTGATCGCCGTGA-1

using 30 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 6, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.299 = AGGGTGATCGTACGGC-1

using 25 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.303 = AGGGTGATCTACTCAT-1

using 83 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.307 = AGGGTGATCTGAGTGT-1

using 77 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 13^3, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.309 = AGGGTGATCTGGCGAC-1

using 4881 reads

====================================================================================

graph has 3178 edges initially, 84 edges after simplification

total ucounts = 346
nonsolo ucounts = 257[2^19, 3^29, 4^18, 5^9, 6^8, 7^11, 8^14, 9^5, 10^2, 11^7, 12^5, 13^8, 14^7, 15^10, 16^7, 17^6, 18^6, 19^8, 20^4, 21^4, 22^2, 23^5, 24^7, 25^3, 26^5, 27^4, 29, 30^2, 31^4, 32^2, 35, 37, 38^2, 41, 43^3, 46^2, 47, 48^2, 50, 55^2, 56, 60, 61, 62, 64, 67, 70, 71, 72, 74, 80, 83, 87, 88, 91, 96, 98, 104, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.311 = AGGGTGATCTGTCTCG-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.312 = AGGGTGATCTTGTTTG-1

using 11 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.313 = AGGTCATAGAAACCAT-1

using 166 reads

====================================================================================

graph has 150 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2, 3^4, 4, 6^2, 7, 8, 21, 23, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.318 = AGGTCATAGACAAGCC-1

using 173 reads

====================================================================================

graph has 170 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 4, 14^2, 30, 101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.320 = AGGTCATAGACTTTCG-1

using 78 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.321 = AGGTCATAGAGCTATA-1

using 50 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.327 = AGGTCATAGCCAGTAG-1

using 2910 reads

====================================================================================

graph has 980 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 5, 11, 22, 2863]
surviving nonsolo ucounts = 2[22, 2863]
ids = [5, 4]

====================================================================================

UMI info for barcode AGGTCATAGCCAGTAG-1 contig 1 = TGGGGCCCTG...
umi CTTCTCCCAG = 2873 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=470]
18-365 ==> 0-347 on |562|TRBV11-1|L-REGION+V-REGION| [len=347] (mis=3)
369-418 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
418-470 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 206 reads
cdr3 = CASSLLGGTDTQYF at 348, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 2814
start codons at 18, 42, 160, 226, 241, 339
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.333 = AGGTCATAGGACCACA-1

using 37 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.334 = AGGTCATAGGAGCGAG-1

using 231 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 226]
surviving nonsolo ucounts = 1[226]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.335 = AGGTCATAGGCCCTTG-1

using 207 reads

====================================================================================

graph has 162 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2^3, 3, 4^2, 6, 8, 10, 13, 17, 22, 37, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.337 = AGGTCATAGGCTAGCA-1

using 53 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[7, 12, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.338 = AGGTCATAGGCTATCT-1

using 34 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.339 = AGGTCATAGGGCTTGA-1

using 35 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.348 = AGGTCATAGTTCGCAT-1

using 78 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.349 = AGGTCATAGTTGAGAT-1

using 132 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 3, 9, 11, 23, 40^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.351 = AGGTCATCAAGAAGAG-1

using 180 reads

====================================================================================

graph has 202 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 17[2^3, 3^3, 4^3, 5, 6^3, 10, 12, 19, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.358 = AGGTCATCAATGGTCT-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.362 = AGGTCATCACCACGTG-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.367 = AGGTCATCACTACAGT-1

using 21074 reads

====================================================================================

graph has 7997 edges initially, 100 edges after simplification

total ucounts = 352
nonsolo ucounts = 238[2^34, 3^24, 4^21, 5^12, 6^15, 7^10, 8^11, 9^9, 10^5, 11^5, 12^5, 13^8, 14^6, 15^3, 16^3, 17^3, 18^6, 19^4, 20, 21^4, 22^3, 23^4, 24^3, 25^2, 27^3, 28^2, 29^3, 30, 32, 34, 36^2, 38, 42, 44^2, 45, 46^2, 56, 57, 69, 82, 296, 493, 531, 557, 1242, 1361, 1451, 1482, 1869, 2147, 2155, 2332, 2405]
surviving nonsolo ucounts = 14[69, 296, 493, 531, 557, 1242, 1361, 1451, 1482, 1869, 2147, 2155, 2332, 2405]
ids = [114, 334, 145, 11, 16, 30, 314, 42, 231, 75, ...]

====================================================================================

UMI info for barcode AGGTCATCACTACAGT-1 contig 1 = GATCTTTTTG...
umi ACGAGCGGGA = 1242 reads: +373 validated
umi ACTGGCTCGC = 229 reads: -334 +1 -2XX +1 -1XX +6 -1XX +1 -1XX +2 -1XX +3 -1XX +4 -5XX +2 -1XX +1 -1XX +4 invalidated
umi CATTTCACAC = 70 reads: +228 -1 +1 -2 +20 -25 +66 -9 +1 -1 +19 non-validated
umi TGTTTCTGCT = 1364 reads: +373 validated
umi TTCTTTCGGA = 291 reads: +373 validated

UMI info for barcode AGGTCATCACTACAGT-1 contig 2 = ATGAGATCCT...
umi AACTATGTCT = 525 reads: +394 validated
umi AATTCGTCGG = 506 reads: +394 validated
umi ACTCAGCCAG = 2345 reads: +394 validated
umi AGATTTAACG = 2146 reads: +394 validated
umi ATTATCAGTC = 1859 reads: +394 validated
umi CAACTAAGCT = 2159 reads: +394 validated
umi CCCAGATACG = 2419 reads: +394 validated
umi CCGTTCGCGT = 501 reads: +70 -1XX +323 invalidated
umi GGATCTTCCC = 1424 reads: -365X +1 -2XX +1 -1XX +1 -3XX +1 -3XX +2 -9XX +2 -1XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=551]
136-449 ==> 0-313 on |469|TRAV16|L-REGION+V-REGION| [len=329] (mis=0)
447-509 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=3)
509-551 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 121 reads
cdr3 = CYSSASKIIF at 448, score = 3 + 9
umis assigned: [30, 42, 114, 314, 334]
of which 5 are surviving nonsolos
reads assigned: 3161
start codons at 31, 70, 136, 299, 442
confident = true

TIG 2[bases=470]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=0)
24-368 ==> 0-344 on |559|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=4)
370-418 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=1)
418-470 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 8 umis using 1020 reads
cdr3 = CAIAGSSNTEAFF at 351, score = 10 + 8
umis assigned: [11, 16, 39, 49, 75, 82, 135, 145, 231]
of which 9 are surviving nonsolos
reads assigned: 13572
start codons at 0, 24, 46, 78, 82, 146, 174, 202, 229, 265
confident = true
now this is a cell
paired!

AAGAAACCATTTGCTCAAGAGGAAGACTCAGCCATGTATTGCTACAGCAGTGCTTCCAAGATAATCTTTGGATCAGGGACCAGACTCAGCATCCGGCCAA <==> TCCGCTACCAGCTCCCAGACATCTGTGTACTTCTGTGCCATCGCAGGGTCCTCGAACACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.369 = AGGTCATCACTGCCAG-1

using 97 reads

====================================================================================

graph has 94 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 3, 23, 31, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.370 = AGGTCATCAGAAGCAC-1

using 79 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 5, 19, 20, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.373 = AGGTCATCAGAGTGTG-1

using 46 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 3, 7, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.376 = AGGTCATCAGCCAGAA-1

using 79 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.378 = AGGTCATCAGCGTAAG-1

using 1351 reads

====================================================================================

graph has 532 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[24, 33, 80, 1212]
surviving nonsolo ucounts = 1[1212]
ids = [4]

====================================================================================

UMI info for barcode AGGTCATCAGCGTAAG-1 contig 1 = ACAGAAGTGG...
umi TCCATTTCTT = 1172 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=511]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
411-469 ==> 8-66 on |429|TRAJ42|J-REGION| [len=66] (mis=0)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CAVQAGGGSQGNLIF at 393, score = 8 + 10
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 1148
start codons at 69, 78
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.392 = AGGTCATCATTGTGCA-1

using 140 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[140]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.402 = AGGTCATGTCAAAGCG-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.403 = AGGTCATGTCAGTGGA-1

using 31 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.406 = AGGTCATGTCCGAGTC-1

using 58 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 8, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.410 = AGGTCATGTCTACCTC-1

using 75 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 7, 16^2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.415 = AGGTCATGTGATGTGG-1

using 4704 reads

====================================================================================

graph has 4460 edges initially, 126 edges after simplification

total ucounts = 518
nonsolo ucounts = 397[2^47, 3^40, 4^37, 5^30, 6^25, 7^23, 8^14, 9^22, 10^12, 11^10, 12^16, 13^9, 14^6, 15^8, 16^8, 17^5, 18^12, 19^9, 20^5, 21^7, 22^6, 23^4, 24^2, 25^8, 26^2, 28^3, 29^4, 30, 31, 33^3, 35^2, 37^4, 40, 44^2, 46, 47, 59, 61, 63, 74, 91, 94, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.416 = AGGTCATGTGCAACGA-1

using 54 reads

====================================================================================

graph has 49 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.418 = AGGTCATGTGTGAATA-1

using 30 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.419 = AGGTCATGTGTTCGAT-1

using 19 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.420 = AGGTCATGTGTTGAGG-1

using 55 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[19, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.424 = AGGTCATGTTATCCGA-1

using 34452 reads

====================================================================================

graph has 11342 edges initially, 168 edges after simplification

total ucounts = 584
nonsolo ucounts = 435[2^53, 3^34, 4^31, 5^25, 6^26, 7^19, 8^22, 9^11, 10^19, 11^20, 12^14, 13^14, 14^13, 15^12, 16^3, 17^10, 18^10, 19^6, 20^6, 21^5, 22^9, 23^3, 24^4, 25^6, 26^3, 27^3, 28^5, 29, 30^2, 31^3, 32, 33^2, 34^2, 36, 37, 38, 40, 41, 44^3, 47^2, 49, 53, 54^3, 64, 76, 85, 86, 92, 99, 122, 962, 1263, 1392, 1403, 1542, 1585, 1609, 1733, 1821, 1836, 1846, 1886, 2163, 2223, 2821, 2872]
surviving nonsolo ucounts = 16[962, 1263, 1392, 1403, 1542, 1585, 1609, 1733, 1821, 1836, 1846, 1886, 2163, 2223, 2821, 2872]
ids = [241, 189, 91, 443, 511, 424, 238, 364, 270, 557, ...]

====================================================================================

UMI info for barcode AGGTCATGTTATCCGA-1 contig 1 = TGGGGACAGT...
umi AATCTCTTGC = 2847 reads: +397 validated
umi CATTAGCACC = 2175 reads: +397 validated
umi CCGGTATGGA = 1255 reads: +397 validated
umi CCTCTCACCT = 1894 reads: +397 validated
umi CGACCGTCAT = 1841 reads: +397 validated
umi CGTCACAACA = 1610 reads: +397 validated
umi CGTTCATCAT = 421 reads: +376 -16 +5 non-validated
umi CTGTCTCTTC = 1830 reads: +397 validated
umi GGGAGTTCTA = 1727 reads: +397 validated
umi GTTGTTATAC = 2223 reads: +397 validated
umi TACCGCATGC = 1594 reads: +397 validated
umi TCAATCCACG = 1400 reads: +397 validated
umi TGGTACCCCT = 1580 reads: +397 validated
umi TTCTGGACCA = 1855 reads: +397 validated

UMI info for barcode AGGTCATGTTATCCGA-1 contig 2 = GACTGTGATT...
umi ACGGATCGTT = 2900 reads: +397 validated
umi ATCACGTTCC = 1398 reads: +397 validated
umi CTTTGGCAAG = 1 reads: -385X +2 -1X +2 -1X +1 -2X +1 -2X invalidated

GOOD CONTIGS

TIG 1[bases=501]
52-399 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=1)
401-449 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
449-501 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 13 umis using 1577 reads
cdr3 = CASSFASEETQYF at 382, score = 7 + 7
umis assigned: [21, 159, 189, 202, 214, 238, 241, 270, 364, 412] and 4 others
of which 14 are surviving nonsolos
reads assigned: 23796
start codons at 52, 76, 161, 260, 373
confident = true

TIG 2[bases=495]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
394-453 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=3)
453-495 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 270 reads
cdr3 = CALSERGFGNVLHC at 380, score = 9 + 6
umis assigned: [45, 91, 279]
of which 2 are surviving nonsolos
reads assigned: 4210
start codons at 15, 56, 131, 219, 273, 408
confident = true
now this is a cell
paired!

CAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTGAGTGAGCGGGGCTTTGGGAATGTGCTGCATTGCGGGTCCGGCACTCAAGTGATTGTTTTACCAC <==> CGCACAGAGCAGGGGGACTCGGCCATGTATCTCTGTGCCAGCAGCTTTGCTAGTGAAGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.425 = AGGTCATGTTCGCTAA-1

using 94 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.426 = AGGTCATGTTCTGTTT-1

using 54 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.427 = AGGTCATGTTGATTCG-1

using 70 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.428 = AGGTCATGTTGGTGGA-1

using 67 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 5, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.432 = AGGTCATTCAAGAAGT-1

using 56 reads

====================================================================================

graph has 107 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 5, 6, 8, 13, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.433 = AGGTCATTCACGAAGG-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.434 = AGGTCATTCAGAGCTT-1

using 14 reads

====================================================================================

graph has 2 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.441 = AGGTCATTCATCTGTT-1

using 54 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.448 = AGGTCATTCCGTAGTA-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.450 = AGGTCATTCCTAGAAC-1

using 86 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[6, 8, 14, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.453 = AGGTCATTCCTGTACC-1

using 85 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.454 = AGGTCATTCCTGTAGA-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.455 = AGGTCATTCCTTCAAT-1

using 59 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.464 = AGGTCATTCTGAAAGA-1

using 48 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 13, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.465 = AGGTCATTCTGGTGTA-1

using 1559 reads

====================================================================================

graph has 602 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2, 3, 4^2, 6, 9, 10, 21, 1496]
surviving nonsolo ucounts = 1[1496]
ids = [0]

====================================================================================

UMI info for barcode AGGTCATTCTGGTGTA-1 contig 1 = AGGACCAGAT...
umi AACCTCACAA = 1489 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=500]
0-51 ==> 23-74 on |591|TRBV2|5'UTR| [len=74] (mis=0)
51-398 ==> 0-347 on |592|TRBV2|L-REGION+V-REGION| [len=347] (mis=5)
398-448 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
448-500 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 111 reads
cdr3 = CASKGTVDQPQHF at 381, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1468
start codons at 8, 51, 68, 150, 262, 292, 319, 372, 424
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.472 = AGGTCCGAGAAGATTC-1

using 1367 reads

====================================================================================

graph has 592 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 5, 1353]
surviving nonsolo ucounts = 1[1353]
ids = [5]

====================================================================================

REJECT CONTIGS

TIG 1[bases=512]
0-76 ==> 0-76 on |487|TRAV25|5'UTR| [len=76] (mis=3)
76-403 ==> 0-327 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=5)
401-470 ==> 0-69 on |439|TRAJ52|J-REGION| [len=69] (mis=1)
470-512 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1340
start codons at 58, 76, 94, 105, 109, 128, 142, 164, 377, 404, 422
confident = false
full length transcript of length 512
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.474 = AGGTCCGAGAGCCCAA-1

using 59 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2^2, 3, 10, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.476 = AGGTCCGAGAGTGAGA-1

using 45 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.477 = AGGTCCGAGATCGGGT-1

using 24 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.485 = AGGTCCGAGCGATATA-1

using 93 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 4, 8, 11, 28, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.486 = AGGTCCGAGCTAGCCC-1

using 43 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2, 3, 4^2, 5^2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.487 = AGGTCCGAGCTCAACT-1

using 23453 reads

====================================================================================

graph has 9986 edges initially, 172 edges after simplification

total ucounts = 1892
nonsolo ucounts = 1145[2^258, 3^150, 4^149, 5^73, 6^88, 7^51, 8^54, 9^46, 10^29, 11^27, 12^27, 13^28, 14^14, 15^22, 16^16, 17^6, 18^11, 19^11, 20^7, 21^4, 22^6, 23^5, 24^7, 25^6, 26^3, 27^3, 28^5, 30, 31^2, 32^5, 33, 34, 35^2, 36, 37^2, 38^3, 40^2, 41^2, 42, 43, 45, 47, 49^2, 50, 53, 54, 62, 70^2, 71, 103, 141, 6793, 6877]
surviving nonsolo ucounts = 2[6793, 6877]
ids = [1272, 256]

====================================================================================

REJECT CONTIGS

TIG 1[bases=404]
47-151 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
151-200 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
200-302 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
352-404 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [256, 1272]
of which 2 are surviving nonsolos
reads assigned: 13500
start codons at 61
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.489 = AGGTCCGAGCTGTCTA-1

using 82 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.490 = AGGTCCGAGGAACTGC-1

using 56 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2^4, 3, 4, 7, 12, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.493 = AGGTCCGAGGATGGTC-1

using 40 reads

====================================================================================

graph has 27 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.500 = AGGTCCGAGTCTCCTC-1

using 21 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.501 = AGGTCCGAGTCTTGCA-1

using 68 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2^3, 3^3, 4^2, 5, 6, 10^2, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.503 = AGGTCCGCAAACTGTC-1

using 28 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.504 = AGGTCCGCAAGCCATT-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 3, 4, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.505 = AGGTCCGCAAGGACAC-1

using 168 reads

====================================================================================

graph has 171 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3^2, 4, 5, 6, 17, 58, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.506 = AGGTCCGCAAGTTCTG-1

using 56 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.508 = AGGTCCGCACACCGCA-1

using 64 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.509 = AGGTCCGCACATCCAA-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.511 = AGGTCCGCACCAGCAC-1

using 33 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.512 = AGGTCCGCACCAGGCT-1

using 75 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 4, 5, 6, 8, 9, 11, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.517 = AGGTCCGCACCTCGTT-1

using 17 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 3, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.521 = AGGTCCGCACGGTAAG-1

using 74 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 13^2, 17, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.522 = AGGTCCGCACGTCTCT-1

using 40 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 3, 8^2, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.523 = AGGTCCGCACGTGAGA-1

using 767 reads

====================================================================================

graph has 455 edges initially, 24 edges after simplification

total ucounts = 30
nonsolo ucounts = 23[2, 3, 4^4, 5^2, 9, 10, 16, 17, 27, 35, 37, 39^2, 52, 80, 86, 87, 88, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.526 = AGGTCCGCAGACGCAA-1

using 36 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 4[4, 6, 8^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.528 = AGGTCCGCAGCGTAAG-1

using 33 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.535 = AGGTCCGCATAGACTC-1

using 8444 reads

====================================================================================

graph has 7642 edges initially, 227 edges after simplification

total ucounts = 1226
nonsolo ucounts = 834[2^165, 3^98, 4^72, 5^58, 6^57, 7^42, 8^42, 9^35, 10^23, 11^18, 12^21, 13^17, 14^18, 15^15, 16^10, 17^18, 18^19, 19^14, 20^10, 21^5, 22^10, 23^8, 24^6, 25^6, 26, 27^5, 28^3, 29^2, 30^2, 31^2, 33, 34, 36^2, 37^2, 39^2, 40, 41^2, 42, 43, 45, 46, 49, 51, 52^2, 54^2, 55, 57, 58, 62^2, 63, 67, 71, 78, 95, 114]
surviving nonsolo ucounts = 1[8]
ids = [603]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.538 = AGGTCCGCATCACCCT-1

using 60 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^3, 4, 6^3, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.543 = AGGTCCGCATGCTAGT-1

using 52 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.544 = AGGTCCGCATGTCCTC-1

using 26 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^3, 3^2, 5, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.546 = AGGTCCGCATTCTCAT-1

using 67 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3^2, 23, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.548 = AGGTCCGGTAAGTAGT-1

using 16 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.551 = AGGTCCGGTACATCCA-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3, 4^3, 10, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.553 = AGGTCCGGTACTTAGC-1

using 66 reads

====================================================================================

graph has 73 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.555 = AGGTCCGGTAGAAGGA-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.557 = AGGTCCGGTAGCTGCC-1

using 83 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[41, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.559 = AGGTCCGGTATATCCG-1

using 17 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.560 = AGGTCCGGTCAACTGT-1

using 35 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2, 3^2, 4, 7, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.563 = AGGTCCGGTCTAGCGC-1

using 7280 reads

====================================================================================

graph has 1684 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 3, 4, 17, 7246]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.564 = AGGTCCGGTCTAGTCA-1

using 27 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.565 = AGGTCCGGTCTGGAGA-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.566 = AGGTCCGGTCTTGCGG-1

using 59 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.568 = AGGTCCGGTCTTTCAT-1

using 62 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.570 = AGGTCCGGTGAGTGAC-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.572 = AGGTCCGGTGCACCAC-1

using 33 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.577 = AGGTCCGGTTATCCGA-1

using 88 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.578 = AGGTCCGGTTATCGGT-1

using 109 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 10, 12, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.579 = AGGTCCGGTTCCCTTG-1

using 29 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.586 = AGGTCCGTCAACGGGA-1

using 37 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.588 = AGGTCCGTCACGGTTA-1

using 300 reads

====================================================================================

graph has 330 edges initially, 8 edges after simplification

total ucounts = 32
nonsolo ucounts = 25[2, 3^5, 4^2, 6^2, 7^2, 8, 9, 11, 12, 13^2, 15, 16, 19, 24, 30, 35, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.589 = AGGTCCGTCACTCCTG-1

using 28 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.590 = AGGTCCGTCAGAGCTT-1

using 37 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.596 = AGGTCCGTCCAACCAA-1

using 39 reads

====================================================================================

graph has 47 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^4, 10, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.598 = AGGTCCGTCCAATGGT-1

using 86 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[3^2, 5, 6, 7, 13, 14, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.600 = AGGTCCGTCCAGTAGT-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.601 = AGGTCCGTCCTAGGGC-1

using 177 reads

====================================================================================

graph has 161 edges initially, 2 edges after simplification

total ucounts = 45
nonsolo ucounts = 25[2^7, 3^3, 4^2, 5^2, 6^2, 7^2, 8, 9, 11^2, 12, 15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.607 = AGGTCCGTCTAACGGT-1

using 43 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3^3, 4, 5, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.618 = AGGTCCGTCTGGCGTG-1

using 2361 reads

====================================================================================

graph has 772 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 2355]
surviving nonsolo ucounts = 1[2355]
ids = [0]

====================================================================================

UMI info for barcode AGGTCCGTCTGGCGTG-1 contig 1 = GGTCACAACT...
umi ACGGACACGG = 2364 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=486]
0-46 ==> 32-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
46-381 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=2)
393-434 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
434-486 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 138 reads
cdr3 = CSASGRAAGEQFF at 367, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 2321
start codons at 46, 184, 220, 226, 239, 299
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.619 = AGGTCCGTCTGTTGAG-1

using 32 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.621 = AGGTCCGTCTTATCTG-1

using 28313 reads

====================================================================================

graph has 10175 edges initially, 284 edges after simplification

total ucounts = 1496
nonsolo ucounts = 1019[2^234, 3^137, 4^101, 5^74, 6^59, 7^52, 8^57, 9^30, 10^19, 11^25, 12^24, 13^19, 14^20, 15^12, 16^12, 17^14, 18^12, 19^6, 20^7, 21^8, 22^8, 23^6, 24^8, 25^8, 26^2, 27^3, 28^5, 29^6, 30^4, 31^2, 32, 33^2, 34^5, 35^2, 36^4, 39, 40, 41^2, 42, 46^2, 47, 48, 51, 57, 58, 66, 69, 70^2, 71, 74, 75, 85, 98, 121, 122, 269, 628, 905, 1444, 1828, 2096, 3554, 8193]
surviving nonsolo ucounts = 8[121, 628, 905, 1444, 1828, 2096, 3554, 8193]
ids = [568, 1332, 1369, 1116, 4, 315, 913, 370]

====================================================================================

UMI info for barcode AGGTCCGTCTTATCTG-1 contig 1 = CTTTTCTCAG...
umi AACAACGTCT = 1824 reads: +391 validated
umi TGGTTCGTCT = 570 reads: -354 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi TTAATCCCCT = 914 reads: +391 validated

UMI info for barcode AGGTCCGTCTTATCTG-1 contig 2 = AGATCTCTGC...
umi CACAAATGAT = 2111 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=474]
0-31 ==> 36-67 on |614|TRBV29-1|5'UTR| [len=67] (mis=0)
31-366 ==> 0-335 on |615|TRBV29-1|L-REGION+V-REGION| [len=335] (mis=2)
360-372 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
375-422 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
422-474 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 190 reads
cdr3 = CSVAQGTFSYEQYF at 352, score = 7 + 8
umis assigned: [4, 1332, 1369]
of which 3 are surviving nonsolos
reads assigned: 3259
start codons at 31, 166, 169, 245, 322
confident = true

TIG 2[bases=464]
0-19 ==> 17-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
19-362 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=2)
360-422 ==> 0-62 on |428|TRAJ41|J-REGION| [len=62] (mis=3)
422-464 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 136 reads
cdr3 = CVVRGSNSGYALNF at 349, score = 6 + 7
umis assigned: [315]
of which 1 are surviving nonsolos
reads assigned: 2079
start codons at 19, 77, 147, 229, 281, 377
confident = true

REJECT CONTIGS

TIG 1[bases=330]
18-217 ==> 5801-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
228-278 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=2)
278-330 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [370, 568, 729, 913]
of which 3 are surviving nonsolos
reads assigned: 11747
start codons at 13, 94, 104, 178, 254
confident = false
did not find CDR3

TIG 2[bases=496]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
394-454 ==> 0-60 on |731|TRAJ15|J-REGION| [len=60] (mis=1)
454-496 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [1116]
of which 1 are surviving nonsolos
reads assigned: 1430
start codons at 15, 56, 131, 219, 273
confident = false
frameshifted full length transcript of length 496
did not find CDR3
now this is a cell
paired!

CAGCTCAGCGATTCAGCCTCCTACATCTGTGTGGTGAGGGGTTCAAATTCCGGGTATGCACTCAACTTCGGCAAAGGCACCTCGCTGTTGGTCACACCCC <==> ATGAGCCCTGAAGACAGCAGCATATATCTCTGCAGCGTTGCACAGGGGACTTTCTCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.622 = AGGTCCGTCTTGCATT-1

using 66 reads

====================================================================================

graph has 70 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[3, 7^2, 9, 13, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.626 = AGTAGTCAGAATGTTG-1

using 197 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 21
nonsolo ucounts = 14[2, 7, 8^3, 9, 11, 12, 13, 15, 17, 25, 26, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.627 = AGTAGTCAGAATTGTG-1

using 69 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.628 = AGTAGTCAGACAATAC-1

using 87 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.633 = AGTAGTCAGATATACG-1

using 37 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.634 = AGTAGTCAGATCACGG-1

using 5766 reads

====================================================================================

graph has 3676 edges initially, 92 edges after simplification

total ucounts = 320
nonsolo ucounts = 228[2^36, 3^34, 4^17, 5^10, 6^12, 7^6, 8^6, 9^6, 10^8, 11^7, 12^5, 13^5, 14^6, 15^9, 16^6, 17^2, 18^4, 19^6, 20^5, 21^2, 22^2, 23^4, 24^3, 25, 26^3, 27^2, 29^2, 30^2, 31, 32, 34, 36^3, 37, 38, 40, 43, 54, 58, 67, 82, 101, 439, 2559]
surviving nonsolo ucounts = 3[2, 439, 2559]
ids = [79, 115, 127]

====================================================================================

UMI info for barcode AGTAGTCAGATCACGG-1 contig 1 = AGAATGCTTA...
umi CCTATGGTCT = 434 reads: +394 validated
umi CGCTCCGTCT = 2586 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=493]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=2)
392-441 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=3)
441-493 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 235 reads
cdr3 = CASSAGMSDTQYF at 374, score = 9 + 8
umis assigned: [115, 127]
of which 2 are surviving nonsolos
reads assigned: 2951
start codons at 3, 47, 169, 255, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.639 = AGTAGTCAGCGATAGC-1

using 33 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.651 = AGTAGTCAGGCATGGT-1

using 32911 reads

====================================================================================

graph has 9017 edges initially, 82 edges after simplification

total ucounts = 342
nonsolo ucounts = 229[2^37, 3^22, 4^17, 5^15, 6^15, 7^11, 8^12, 9^14, 10^3, 11^4, 12^4, 13, 14^4, 15^3, 16^3, 17^8, 18^5, 19^3, 20, 21, 22^5, 23^4, 24^2, 26^2, 27, 28, 30^2, 31, 32, 33, 34, 48, 64, 69, 77, 79, 95, 110, 181, 334, 419, 679, 691, 1431, 1462, 1619, 1722, 1759, 1946, 2149, 2202, 2292, 2377, 2574, 2911, 3681]
surviving nonsolo ucounts = 16[334, 419, 691, 1431, 1462, 1619, 1722, 1759, 1946, 2149, 2202, 2292, 2377, 2574, 2911, 3681]
ids = [188, 29, 200, 218, 87, 181, 84, 263, 94, 154, ...]

====================================================================================

UMI info for barcode AGTAGTCAGGCATGGT-1 contig 1 = AGACCAGAAT...
umi ACACCCCTGT = 639 reads: -384 +2 -1X +2 -1XX +2 -2XX +1 -2XX +1 -1XX +2 -1XX +1 invalidated
umi ACGCTATCCC = 402 reads: +403 validated
umi AGGAATATTG = 2288 reads: +403 validated
umi ATTTTATAAC = 1722 reads: +403 validated
umi CAAAGACGGC = 1467 reads: +403 validated
umi CACTATACAG = 1957 reads: +403 validated
umi CATATTAGCT = 2387 reads: +403 validated
umi CTACGGAGGG = 2145 reads: +403 validated
umi CTCGATCGTC = 2904 reads: +403 validated
umi CTGTCAGTCA = 3587 reads: +403 validated
umi GAAGCTCATC = 329 reads: +403 validated
umi GCAACCCTCC = 687 reads: +403 validated
umi GCCATACTTT = 2564 reads: +403 validated
umi GCGCATCATC = 1434 reads: +403 validated
umi TAGGATTCCC = 1767 reads: +403 validated
umi TATCGCATAC = 2201 reads: +403 validated

UMI info for barcode AGTAGTCAGGCATGGT-1 contig 2 = GGGGGGACTC...
umi CTTTCACCTA = 1614 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=0)
400-450 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=2)
450-502 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 15 umis using 2002 reads
cdr3 = CASSQDLGPGNQPQHF at 374, score = 9 + 8
umis assigned: [23, 29, 42, 84, 87, 94, 109, 154, 164, 172] and 6 others
of which 15 are surviving nonsolos
reads assigned: 28035
start codons at 47, 146, 169, 189, 197, 239, 273, 426
confident = true

TIG 2[bases=511]
75-411 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=2)
411-469 ==> 0-58 on |420|TRAJ34|J-REGION| [len=58] (mis=0)
469-511 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CAVSSYNTDKLIF at 399, score = 6 + 9
umis assigned: [181]
of which 1 are surviving nonsolos
reads assigned: 1595
start codons at 75, 145, 282, 307
confident = true
now this is a cell
paired!

TCCCATCCCAGAGACTCTGCCGTCTACATCTGTGCTGTCTCATCTTATAACACCGACAAGCTCATCTTTGGGACTGGGACCAGATTACAAGTCTTTCCAA <==> CTTGGTGACTCTGCTGTGTATTTCTGTGCCAGCAGCCAAGATCTGGGGCCCGGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.653 = AGTAGTCAGGCCCTCA-1

using 29 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.656 = AGTAGTCAGGCTCTTA-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.667 = AGTAGTCAGTGGGTTG-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.670 = AGTAGTCAGTTCGCGC-1

using 117 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.672 = AGTAGTCCAACACCTA-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.677 = AGTAGTCCAATAAGCA-1

using 160 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 43, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.678 = AGTAGTCCAATGTTGC-1

using 94 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.679 = AGTAGTCCACCAGTTA-1

using 81 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.681 = AGTAGTCCACTCGACG-1

using 87 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.685 = AGTAGTCCAGAGCCAA-1

using 1906 reads

====================================================================================

graph has 689 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[1903]
surviving nonsolo ucounts = 1[1903]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCCAGAGCCAA-1 contig 1 = GTTCCCCTAT...
umi GATGGGACGA = 1914 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=509]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=1)
416-457 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
457-509 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 111 reads
cdr3 = CASSEGRWEVGEQFF at 384, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1873
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.686 = AGTAGTCCAGATAATG-1

using 72495 reads

====================================================================================

graph has 18834 edges initially, 214 edges after simplification

total ucounts = 603
nonsolo ucounts = 407[2^61, 3^35, 4^27, 5^29, 6^19, 7^19, 8^8, 9^14, 10^12, 11^14, 12^6, 13^11, 14^8, 15^7, 16^5, 17^8, 18^3, 19^7, 20^7, 21^3, 23^4, 24^3, 25^2, 26^5, 27^4, 28^4, 29^2, 30^5, 31^5, 32^3, 33^3, 34^3, 35^2, 36, 37^4, 38^2, 39^2, 42, 44, 47, 48, 49^2, 50, 51, 53, 55, 57, 58, 60, 63^2, 65^2, 66, 70, 71, 77, 84, 95, 102, 324, 371, 544, 633, 757, 994, 1014, 1095, 1139, 1185, 1217, 1344, 1357, 1522, 1702, 1717, 1843, 1948, 2061, 2210, 4318, 5334, 6888, 7059, 8175, 10202]
surviving nonsolo ucounts = 25[324, 371, 544, 633, 994, 1014, 1095, 1139, 1185, 1217, 1344, 1357, 1522, 1702, 1717, 1843, 1948, 2061, 2210, 4318, 5334, 6888, 7059, 8175, 10202]
ids = [313, 20, 587, 470, 75, 315, 179, 64, 454, 532, ...]

====================================================================================

UMI info for barcode AGTAGTCCAGATAATG-1 contig 1 = GGGGCTTTTT...
umi ACTACCTGCT = 1523 reads: +403 validated
umi ATCGTAACCT = 1139 reads: +403 validated
umi ATTATATTTT = 989 reads: +403 validated
umi CAACGTTACT = 7098 reads: -71X +2 -1XX +329 invalidated
umi CCGCATTCAC = 2332 reads: -318X +2 -1XX +1 -3XX +1 -2XX +2 -9XX +1 -3XX +60 invalidated
umi CTTACTCTAT = 1016 reads: +403 validated
umi TTTACAAAGA = 550 reads: +403 validated

UMI info for barcode AGTAGTCCAGATAATG-1 contig 2 = GAATGGAGGC...
umi ACTTTTTCTT = 2281 reads: +26 -2XX +363 invalidated
umi ATTAATCCCC = 1937 reads: +391 validated
umi CCCGGATAGC = 1092 reads: +391 validated
umi CCTTCATCAC = 1723 reads: +391 validated
umi TGTATATTCG = 1849 reads: +391 validated
umi TTGTCCCTTA = 1349 reads: +391 validated
umi TTTAGGTATG = 8360 reads: -315X +1 -6XX +1 -2XX +1 -2XX +1 -3XX +2 -1XX +1 -9XX +1 -1XX +44 invalidated

UMI info for barcode AGTAGTCCAGATAATG-1 contig 3 = TTCCTCTGCT...
umi ACACGCAATA = 6520 reads: -350 +1 -2XX +1 -2XX +3 -1XX +2 -4XX +9 -1XX +1 -1XX +2 -1XX +2 -3XX +2 invalidated
umi ACCCTCAGTA = 310 reads: -369X +1 -3X +1 -2X +1 -2XX +2 -1XX +1 -2XX +3 invalidated
umi CACAGTCCCG = 1344 reads: +388 validated
umi CTTACACTCT = 275 reads: -369X +6 -1X +1 -1XX +2 -1XX +2 -3XX +2 invalidated
umi GGTCTCCTTA = 4881 reads: -366 +6 -1XX +2 -1XX +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi TAGCAGATCT = 1173 reads: +388 validated

UMI info for barcode AGTAGTCCAGATAATG-1 contig 4 = GTTCTCTGGA...
umi TATGAATGTT = 634 reads: +391 validated
umi TGATATTTAA = 1213 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=553]
108-445 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=0)
449-511 ==> 0-62 on |446|TRAJ6|J-REGION| [len=62] (mis=2)
511-553 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 819 reads
cdr3 = CVVNTYPSGGSYIPTF at 432, score = 7 + 7
umis assigned: [32, 64, 75, 96, 195, 315, 587]
of which 7 are surviving nonsolos
reads assigned: 14376
start codons at 10, 47, 105, 108, 205, 312, 334, 340
confident = true

TIG 2[bases=502]
0-59 ==> 19-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
59-394 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=5)
394-406 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=0)
400-450 ==> 0-50 on |538|TRBJ1-3|J-REGION| [len=50] (mis=5)
450-502 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 622 reads
cdr3 = CSAKTGQGANTIYF at 380, score = 7 + 7
umis assigned: [42, 72, 179, 224, 547, 585, 591]
of which 7 are surviving nonsolos
reads assigned: 18252
start codons at 2, 59, 197, 233, 239, 252, 312
confident = true

TIG 3[bases=552]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=5)
443-455 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
459-500 ==> 7-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
500-552 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 96 reads
cdr3 = CAWLNRGVRGYTF at 433, score = 7 + 9
umis assigned: [13, 20, 103, 313, 407, 454]
of which 6 are surviving nonsolos
reads assigned: 14359
start codons at 109, 112, 180
confident = true

TIG 4[bases=513]
0-80 ==> 129-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
80-401 ==> 0-321 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=0)
405-471 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=1)
471-513 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 127 reads
cdr3 = CIVRPYSGGGADGLTF at 392, score = 8 + 8
umis assigned: [470, 532]
of which 2 are surviving nonsolos
reads assigned: 1813
start codons at 80, 132, 158, 219, 225, 276, 291, 300, 305
confident = true

REJECT CONTIGS

TIG 1[bases=509]
62-409 ==> 0-347 on |703|TRDV1|L-REGION+V-REGION| [len=347] (mis=0)
404-467 ==> 0-63 on |443|TRAJ57|J-REGION| [len=63] (mis=1)
467-509 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CSWGTQGGSEKLVF at 394, score = 3 + 9
umis assigned: [347, 352]
of which 2 are surviving nonsolos
reads assigned: 3718
start codons at 55, 62, 158, 195, 254, 282, 291
confident = false
not full
not full

TIG 2[bases=342]
0-139 ==> 37-176 on |496|TRAV3|5'UTR| [len=176] (mis=1)
139-300 ==> 0-161 on |497|TRAV3|L-REGION+V-REGION| [len=343] (mis=0)
300-342 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [391]
of which 1 are surviving nonsolos
reads assigned: 6825
start codons at 139, 160, 169, 261
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.688 = AGTAGTCCAGATGGGT-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.691 = AGTAGTCCAGCTCGAC-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.705 = AGTAGTCCATGCCTAA-1

using 48 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.707 = AGTAGTCCATTCACTT-1

using 120 reads

====================================================================================

graph has 30 edges initially, 6 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.708 = AGTAGTCCATTCCTGC-1

using 19 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.710 = AGTAGTCCATTGAGCT-1

using 36 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.711 = AGTAGTCCATTGCGGC-1

using 5493 reads

====================================================================================

graph has 5230 edges initially, 112 edges after simplification

total ucounts = 714
nonsolo ucounts = 478[2^86, 3^68, 4^42, 5^31, 6^28, 7^32, 8^15, 9^19, 10^10, 11^9, 12^9, 13^13, 14^10, 15^11, 16^18, 17^6, 18^13, 19^10, 20^6, 21, 22, 23, 24^2, 25, 27^3, 28^3, 30, 31, 32^2, 34^3, 35^2, 36^3, 42^2, 43, 45, 50, 59, 60, 62, 74, 75, 76, 98, 99, 102, 111, 119, 130, 164]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.713 = AGTAGTCGTAAACACA-1

using 2585 reads

====================================================================================

graph has 860 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[16, 17, 2548]
surviving nonsolo ucounts = 1[2548]
ids = [6]

====================================================================================

UMI info for barcode AGTAGTCGTAAACACA-1 contig 1 = ACCTGGAGCC...
umi TCACCTTTTT = 2567 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=495]
0-43 ==> 0-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
43-387 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=3)
393-443 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
443-495 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 166 reads
cdr3 = CASSLLSQTDNEQFF at 370, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 2520
start codons at 35, 43, 65, 178, 185, 193, 247, 251, 275, 401
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.715 = AGTAGTCGTAAGTGTA-1

using 225 reads

====================================================================================

graph has 146 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2, 3, 6, 9^2, 10^2, 15, 40, 44, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.716 = AGTAGTCGTACGCTGC-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.717 = AGTAGTCGTAGATTAG-1

using 95 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2^2, 5^2, 7, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.719 = AGTAGTCGTCAGCTAT-1

using 12524 reads

====================================================================================

graph has 6212 edges initially, 130 edges after simplification

total ucounts = 626
nonsolo ucounts = 446[2^72, 3^47, 4^35, 5^24, 6^34, 7^30, 8^28, 9^17, 10^18, 11^18, 12^9, 13^9, 14^10, 15^9, 16^8, 17^9, 18^3, 19^9, 20^9, 21^5, 22^3, 23^3, 24^2, 26^3, 27, 29^3, 30, 31^2, 32, 33, 34^2, 35, 36^2, 39^2, 40^2, 41, 42, 44, 49, 54, 67, 70^2, 72, 77, 78, 82, 95, 7633]
surviving nonsolo ucounts = 1[7633]
ids = [283]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.720 = AGTAGTCGTCCTGCTT-1

using 47 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[6, 10, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.721 = AGTAGTCGTCGAGTTT-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.722 = AGTAGTCGTCGCTTCT-1

using 22 reads

====================================================================================

graph has 33 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.724 = AGTAGTCGTCTTGTCC-1

using 103 reads

====================================================================================

graph has 68 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3, 18, 21, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.726 = AGTAGTCGTGCGGTAA-1

using 39 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.733 = AGTAGTCTCACAACGT-1

using 45 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.736 = AGTAGTCTCAGCATGT-1

using 170 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[170]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.740 = AGTAGTCTCATCACCC-1

using 2395 reads

====================================================================================

graph has 922 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4, 9, 11, 2366]
surviving nonsolo ucounts = 1[2366]
ids = [5]

====================================================================================

REJECT CONTIGS

TIG 1[bases=320]
0-209 ==> 138-347 on |673|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=2)
219-268 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
268-320 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSLVVGASTDTQYF at 192, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1773
start codons at 
confident = false
not full
VJ delta = 18
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.743 = AGTAGTCTCATTCACT-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.745 = AGTAGTCTCCAAACTG-1

using 103 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.747 = AGTAGTCTCCACGAAT-1

using 128 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.749 = AGTAGTCTCCAGTAGT-1

using 49 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.750 = AGTAGTCTCCATGAAC-1

using 114 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[42, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.754 = AGTAGTCTCCGCATCT-1

using 94 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.756 = AGTAGTCTCCGGCACA-1

using 19 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.759 = AGTAGTCTCCGTTGTC-1

using 2726 reads

====================================================================================

graph has 2125 edges initially, 58 edges after simplification

total ucounts = 232
nonsolo ucounts = 174[2^27, 3^21, 4^13, 5^10, 6^13, 7^4, 8^10, 9^5, 10^4, 11^3, 12^6, 13^3, 14^7, 15^5, 16^4, 17^5, 18, 19^6, 20^3, 21^2, 23^3, 24, 25^2, 26^2, 27, 29, 30^2, 32, 34, 36, 37, 38, 41, 49, 60, 83, 757]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=483]
0-66 ==> 0-66 on |451|TRAV1-1|5'UTR| [len=66] (mis=0)
66-392 ==> 0-326 on |452|TRAV1-1|L-REGION+V-REGION| [len=326] (mis=5)
390-441 ==> 10-61 on |450|TRAJ9|J-REGION| [len=61] (mis=0)
441-483 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [170]
of which 0 are surviving nonsolos
reads assigned: 728
start codons at 33, 49, 66, 88, 96, 102, 205, 229, 232, 262, 271, 325, 354
confident = false
frameshifted full length transcript of length 483
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.760 = AGTAGTCTCCTACAGA-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.761 = AGTAGTCTCCTCAATT-1

using 47 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.763 = AGTAGTCTCCTTTACA-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.764 = AGTAGTCTCGACGGAA-1

using 12 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.768 = AGTAGTCTCGCTAGCG-1

using 115 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 15, 19, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.769 = AGTAGTCTCGGAAATA-1

using 36 reads

====================================================================================

graph has 17 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.773 = AGTAGTCTCGGTGTCG-1

using 36 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 3, 7, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.774 = AGTAGTCTCGGTGTTA-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.775 = AGTAGTCTCGTCACGG-1

using 124 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.778 = AGTAGTCTCTAACCGA-1

using 34556 reads

====================================================================================

graph has 11597 edges initially, 112 edges after simplification

total ucounts = 384
nonsolo ucounts = 266[2^28, 3^29, 4^23, 5^13, 6^10, 7^12, 8^15, 9^3, 10^6, 11^10, 12^8, 13^9, 14^4, 15^5, 16^7, 17^3, 18^8, 19^6, 20^6, 21^5, 22, 23, 26^2, 27, 28^2, 29, 30^2, 31^2, 32, 33, 37, 40, 41^2, 42, 46, 49, 50, 51, 55, 59, 60, 61, 64, 68, 70, 71, 74^2, 78, 79, 81, 82, 86, 93, 102, 107, 127, 158, 506, 997, 1245, 1359, 1578, 1636, 1873, 1935, 2040, 2068, 2254, 3383, 9371]
surviving nonsolo ucounts = 14[127, 506, 997, 1245, 1359, 1578, 1636, 1873, 1935, 2040, 2068, 2254, 3383, 9371]
ids = [319, 351, 15, 228, 118, 22, 194, 48, 6, 37, ...]

====================================================================================

UMI info for barcode AGTAGTCTCTAACCGA-1 contig 1 = GTCATCCCTC...
umi AACTGCCATA = 1952 reads: +388 validated
umi AATATCAGTC = 982 reads: +388 validated
umi ACAATATCGT = 1587 reads: +388 validated
umi ACGACTGCCG = 2058 reads: +388 validated
umi ACTTTATTAC = 1884 reads: +388 validated
umi CTTACATCTT = 1644 reads: +388 validated
umi GATATGCCCG = 1253 reads: +388 validated
umi TGCACTGATA = 2081 reads: +388 validated
umi TGTCATATGT = 508 reads: +388 validated

UMI info for barcode AGTAGTCTCTAACCGA-1 contig 2 = AGTAAAGAGA...
umi CCAATCTGGA = 1372 reads: +397 validated
umi TACAATCATC = 2260 reads: +397 validated
umi TCGAACGTCA = 119 reads: -376 +1 -2X +1 -1XX +2 -5XX +2 -3XX +1 -3XX invalidated

GOOD CONTIGS

TIG 1[bases=518]
0-78 ==> 0-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=2)
418-466 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=4)
466-518 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 9 umis using 890 reads
cdr3 = CSAKDRIFTEAFF at 399, score = 7 + 8
umis assigned: [6, 15, 22, 37, 48, 194, 228, 340, 351]
of which 9 are surviving nonsolos
reads assigned: 13654
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

TIG 2[bases=514]
0-75 ==> 72-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
75-412 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=0)
421-472 ==> 12-63 on |398|TRAJ13|J-REGION| [len=63] (mis=2)
472-514 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 180 reads
cdr3 = CATDALGSYQKVTF at 399, score = 6 + 8
umis assigned: [118, 282, 319]
of which 3 are surviving nonsolos
reads assigned: 3684
start codons at 75, 110, 187, 195, 289
confident = true

REJECT CONTIGS

TIG 1[bases=303]
4-199 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
201-251 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
251-303 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [132, 163, 204]
of which 2 are surviving nonsolos
reads assigned: 12330
start codons at 76, 86, 160, 209
confident = false
did not find CDR3
now this is a cell
paired!

CGGGCAGCAGACACTGCTTCTTACTTCTGTGCTACGGACGCCCTTGGGAGTTACCAGAAAGTTACCTTTGGAATTGGAACAAAGCTCCAAGTCATCCGAA <==> AGTGCCCATCCTGAAGACAGCAGCTTCTACATCTGCAGTGCTAAGGACAGGATTTTTACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.781 = AGTAGTCTCTCCCTGA-1

using 382 reads

====================================================================================

graph has 276 edges initially, 10 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 9, 24, 55, 88, 99, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.782 = AGTAGTCTCTCTTGAT-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.789 = AGTCTTTAGAACTCGG-1

using 24 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.791 = AGTCTTTAGAAGGACA-1

using 64 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.793 = AGTCTTTAGACAATAC-1

using 34 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.794 = AGTCTTTAGACAGACC-1

using 74 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 4, 6, 17, 18, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.795 = AGTCTTTAGACCTTTG-1

using 29 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 3, 4, 7, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.796 = AGTCTTTAGAGAACAG-1

using 45 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.798 = AGTCTTTAGATCCTGT-1

using 933 reads

====================================================================================

graph has 720 edges initially, 22 edges after simplification

total ucounts = 125
nonsolo ucounts = 69[2^10, 3^9, 4^6, 5^5, 6^3, 7, 8^4, 9^3, 11, 12^2, 13, 14, 15, 16, 17, 18, 20^2, 21, 23, 25^3, 26^2, 29, 30^2, 34, 35, 36, 37, 39, 43, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.799 = AGTCTTTAGCAGACTG-1

using 115 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.806 = AGTCTTTAGCGATATA-1

using 237 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^3, 3^2, 5, 14, 15, 28, 30, 41, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.809 = AGTCTTTAGCTAGTCT-1

using 139 reads

====================================================================================

graph has 56 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[4, 18, 26, 40, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.810 = AGTCTTTAGCTGTCTA-1

using 117 reads

====================================================================================

graph has 115 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2^3, 3, 4^2, 5^2, 6^2, 9, 12^2, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.815 = AGTCTTTAGGCATGGT-1

using 34 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 6, 7, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.816 = AGTCTTTAGGGAACGG-1

using 38753 reads

====================================================================================

graph has 11383 edges initially, 288 edges after simplification

total ucounts = 738
nonsolo ucounts = 575[2^79, 3^49, 4^30, 5^33, 6^27, 7^19, 8^28, 9^25, 10^11, 11^19, 12^15, 13^18, 14^13, 15^14, 16^7, 17^13, 18^8, 19^8, 20^10, 21^7, 22^7, 23^11, 24^3, 25^6, 26^5, 27^4, 28^8, 29^4, 30, 31^3, 32^3, 33^3, 34^2, 35, 36^2, 38^3, 39, 41, 42, 43, 45^2, 46^2, 47^4, 48^3, 49^2, 52, 53, 54, 56^2, 57^2, 58^2, 59, 60^3, 61, 63, 64, 65^2, 66^3, 68^2, 70, 71^2, 77^3, 80, 87, 90, 93, 95, 110, 111, 316, 402, 612, 720, 962, 967, 1064, 1190, 1296, 1343, 1351, 1418, 1467, 1514, 1528, 1614, 1616, 1624, 1640, 1684, 1847, 1892, 1996]
surviving nonsolo ucounts = 24[66, 316, 402, 612, 720, 962, 967, 1064, 1190, 1296, 1343, 1351, 1418, 1467, 1514, 1528, 1614, 1616, 1624, 1640, 1684, 1847, 1892, 1996]
ids = [585, 162, 155, 665, 45, 618, 97, 336, 311, 225, ...]

====================================================================================

UMI info for barcode AGTCTTTAGGGAACGG-1 contig 1 = ATGCTCACAG...
umi AACTCTGTCG = 1513 reads: +400 validated
umi AGAGTATTGT = 1358 reads: +400 validated
umi ATCTTAGCCA = 398 reads: +400 validated
umi CATATACGGT = 1306 reads: +400 validated
umi CCACATTTGG = 1900 reads: +400 validated
umi CCTGACAGTT = 1526 reads: +400 validated
umi CCTTCTCGGT = 1432 reads: +400 validated
umi CGCTGCATTC = 1614 reads: +400 validated
umi CGTGATATTT = 1223 reads: +25 -1XX +374 invalidated
umi GGTTTTCGTC = 1639 reads: +400 validated
umi GTAAGTAACG = 1454 reads: +400 validated
umi TACACTGGGT = 1620 reads: +400 validated
umi TAGTCGGATC = 1849 reads: +400 validated
umi TATTTTTTGA = 2002 reads: +400 validated
umi TCCTCCTGGT = 1689 reads: +400 validated
umi TCTGAACGAG = 964 reads: +400 validated
umi TGGTAAGCGG = 1359 reads: +400 validated
umi TTCAGATCAC = 1637 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=504]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-399 ==> 0-347 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=4)
408-452 ==> 6-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
452-504 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 17 umis using 1521 reads
cdr3 = CASSTQQGGNEQFF at 382, score = 8 + 8
umis assigned: [25, 103, 155, 225, 249, 277, 280, 297, 311, 466] and 8 others
of which 18 are surviving nonsolos
reads assigned: 26045
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320, 410
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.818 = AGTCTTTAGGGTTCCC-1

using 31 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.821 = AGTCTTTAGTAAGTAC-1

using 117 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.822 = AGTCTTTAGTAATCCC-1

using 75 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3, 4, 5, 6^3, 7, 9, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.824 = AGTCTTTAGTAGCGGT-1

using 47 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.829 = AGTCTTTAGTGCAAGC-1

using 67 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.830 = AGTCTTTAGTGCGATG-1

using 33 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.831 = AGTCTTTAGTGTTAGA-1

using 59 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[3, 4^2, 7, 8, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.833 = AGTCTTTAGTTAGCGG-1

using 31049 reads

====================================================================================

graph has 13050 edges initially, 407 edges after simplification

total ucounts = 1344
nonsolo ucounts = 1024[2^143, 3^112, 4^65, 5^76, 6^63, 7^48, 8^43, 9^44, 10^48, 11^29, 12^27, 13^32, 14^13, 15^17, 16^27, 17^16, 18^19, 19^12, 20^15, 21^14, 22^15, 23^12, 24^9, 25^10, 26^9, 27^7, 28^8, 29^11, 30, 31^2, 32^4, 33^4, 34^2, 35^2, 36^4, 37^5, 38^2, 39, 40^2, 41^2, 42^2, 43^3, 44, 45^6, 46^2, 47^8, 49^4, 50^2, 51, 52, 53, 54^2, 55^2, 57, 62, 63, 66, 71, 72, 75, 76^2, 81, 88, 103, 8010, 10470]
surviving nonsolo ucounts = 4[6, 10, 8010, 10470]
ids = [1244, 1193, 244, 1240]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.838 = AGTCTTTAGTTTCCTT-1

using 58 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.841 = AGTCTTTCAAGGACTG-1

using 20 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.843 = AGTCTTTCAAGGTGTG-1

using 79 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.847 = AGTCTTTCAATTCCTT-1

using 77 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 9[2^2, 3^3, 4, 6, 14, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.850 = AGTCTTTCACAGGCCT-1

using 45 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.851 = AGTCTTTCACCAGGCT-1

using 22 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.853 = AGTCTTTCACCGAATT-1

using 55 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.856 = AGTCTTTCACCTGGTG-1

using 28 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.857 = AGTCTTTCACGACTCG-1

using 75 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.859 = AGTCTTTCACGGCGTT-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 4, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.860 = AGTCTTTCACGTGAGA-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.861 = AGTCTTTCACTACAGT-1

using 36 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.865 = AGTCTTTCAGAGCCAA-1

using 113 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3^2, 16, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.876 = AGTCTTTCATCACCCT-1

using 32 reads

====================================================================================

graph has 26 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3, 7, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.877 = AGTCTTTCATCACGTA-1

using 18685 reads

====================================================================================

graph has 9469 edges initially, 305 edges after simplification

total ucounts = 948
nonsolo ucounts = 714[2^103, 3^64, 4^53, 5^43, 6^41, 7^32, 8^28, 9^28, 10^28, 11^21, 12^25, 13^16, 14^17, 15^19, 16^9, 17^15, 18^11, 19^14, 20^8, 21^15, 22^11, 23^11, 24^12, 25^13, 26^6, 27^5, 28^3, 29^5, 30^5, 31^4, 32^4, 33^5, 34^3, 35^3, 36^3, 37, 38^3, 39, 40^4, 41^2, 43^2, 44, 46, 47, 49^2, 51, 52, 53^2, 59, 62, 66^2, 74, 80, 98, 2808, 7015]
surviving nonsolo ucounts = 2[2808, 7015]
ids = [106, 902]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.879 = AGTCTTTCATCCGCGA-1

using 844 reads

====================================================================================

graph has 613 edges initially, 18 edges after simplification

total ucounts = 36
nonsolo ucounts = 30[2^4, 3^2, 5^2, 6, 7, 10, 11, 15^2, 16, 22, 31, 35, 36, 38^2, 39, 42, 46, 54, 66^3, 76, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.886 = AGTCTTTCATGGTCAT-1

using 118 reads

====================================================================================

graph has 80 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[3, 8^2, 10, 18, 20, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.888 = AGTCTTTCATTGGGCC-1

using 66 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.889 = AGTCTTTCATTTGCCC-1

using 63 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.890 = AGTCTTTCATTTGCTT-1

using 78 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.891 = AGTCTTTGTAAACACA-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.892 = AGTCTTTGTAAAGTCA-1

using 53 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^4, 11, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.894 = AGTCTTTGTAAGGATT-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.897 = AGTCTTTGTAATCACC-1

using 72 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 15, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.898 = AGTCTTTGTAATCGTC-1

using 77 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.900 = AGTCTTTGTACGAAAT-1

using 39 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.906 = AGTCTTTGTATGGTTC-1

using 66077 reads

====================================================================================

graph has 22804 edges initially, 482 edges after simplification

total ucounts = 1468
nonsolo ucounts = 1133[2^157, 3^106, 4^87, 5^83, 6^38, 7^46, 8^46, 9^47, 10^32, 11^23, 12^31, 13^17, 14^30, 15^32, 16^26, 17^28, 18^24, 19^18, 20^16, 21^20, 22^20, 23^14, 24^11, 25^12, 26^3, 27^18, 28^7, 29^10, 30^8, 31^5, 32^7, 33^5, 34^7, 35^6, 36^4, 37^3, 38^4, 39^4, 40^2, 41, 42, 44^3, 45^4, 46, 47, 48, 49, 50, 52^3, 54, 55, 58, 66, 67^2, 73, 74, 75, 84, 85, 89, 91, 111, 123, 125, 126, 172, 384, 402, 405, 419, 426, 481, 490, 572, 639, 655, 698, 715, 762, 773, 874, 878, 891, 977^2, 1023, 1050, 1063, 1088, 1094, 1100, 1222, 1276, 1287, 1307, 1343, 1350, 1397, 1458, 1460, 1555, 1756, 1796, 1819, 1964, 1973, 10098]
surviving nonsolo ucounts = 41[384, 402, 405, 419, 426, 481, 490, 572, 639, 655, 698, 715, 762, 773, 874, 878, 891, 977^2, 1023, 1050, 1063, 1088, 1094, 1100, 1222, 1276, 1287, 1307, 1343, 1350, 1397, 1458, 1460, 1555, 1756, 1796, 1819, 1964, 1973, 10098]
ids = [1338, 479, 672, 1047, 369, 821, 993, 1006, 930, 688, ...]

====================================================================================

UMI info for barcode AGTCTTTGTATGGTTC-1 contig 1 = TTCAGCTCTG...
umi AAACTGCGCC = 1226 reads: -386 +1 -11XX +2 invalidated
umi AACTCTATTC = 898 reads: +388 -2 +10 non-validated
umi AATGTCGCTA = 969 reads: +387 -7 +6 non-validated
umi ACAGTGTGCG = 948 reads: +369 -26 +5 non-validated
umi ACGGTTTTGG = 7281 reads: -378 +5 -1XX +2 -1XX +3 -1XX +2 -1XX +1 -1XX +4 invalidated
umi ACTACTTGTG = 2 reads: -397X +1 -2XX invalidated
umi AGGCTCTCCT = 1312 reads: -385X +2 -11XX +2 invalidated
umi AGGTCCTGGG = 1 reads: -396 +1 -3X invalidated
umi CAACAAGACT = 783 reads: +340 -56 +4 non-validated
umi CAATTATGCT = 356 reads: -385X +2 -11X +2 invalidated
umi CATCTGCATT = 1 reads: -397X +1 -2X invalidated
umi CCCTCTTTTA = 1220 reads: +392 -1 +7 non-validated
umi CGACGTTATT = 1227 reads: -385X +2 -11XX +2 invalidated
umi CTTGAAAGGG = 876 reads: +379 -3 +1 -1 +1 -1 +5 -1 +8 non-validated
umi CTTTATCCCC = 402 reads: +333 -1 +14 -1 +2 -1 +3 -1 +2 -36 +6 non-validated
umi GAATTATAAC = 638 reads: +325 -70 +5 non-validated
umi GATCAACCAC = 1087 reads: +400 validated
umi GTTAGCACGT = 804 reads: -385X +2 -11XX +2 invalidated
umi GTTCTGTGGG = 618 reads: +301 -10 +60 -1 +7 -1 +20 non-validated
umi TACTAGGTCA = 532 reads: -21 +1 -16XX +2 -2XX +1 -1XX +1 -1XX +342 -1 +11 invalidated
umi TACTTGGGCG = 574 reads: +319 -2 +1 -3 +9 -59 +7 non-validated
umi TATGCCCGTC = 1826 reads: +400 validated
umi TATGGTTCTG = 416 reads: +400 validated
umi TCAATTCTAT = 684 reads: +336 -59 +5 non-validated
umi TCGTTCTTTA = 1102 reads: +400 validated
umi TTCACTGTCA = 335 reads: -388 +2 -1XX +2 -1XX +1 -1XX +4 invalidated
umi TTCGCGGCAT = 1020 reads: +400 validated

UMI info for barcode AGTCTTTGTATGGTTC-1 contig 2 = AGATCAGAAG...
umi ACCACAATGC = 1764 reads: +421 validated
umi ACGGACCGAC = 70 reads: -403X +1 -2XX +1 -2XX +7 -1XX +4 invalidated
umi ACTTAACGCG = 1803 reads: +421 validated
umi ATCCAGCGGG = 763 reads: +421 validated
umi ATTAACGCCC = 26 reads: -400 +1 -2X +1 -2XX +1 -2XX +7 -1XX +4 invalidated
umi CCAGCCCTCA = 1970 reads: +421 validated
umi CCGCCTGCGT = 1067 reads: +421 validated
umi CCTAAGCCTA = 404 reads: +421 validated
umi GCATCGATTG = 1394 reads: +421 validated
umi GGCACCGTGA = 487 reads: +421 validated
umi GTACGGACGG = 1309 reads: +421 validated
umi TAACTGGCGC = 1098 reads: +421 validated
umi TCAGTGATTA = 1561 reads: +421 validated
umi TCGCTAATAG = 1320 reads: +279 -2X +140 invalidated
umi TGAGGCCACA = 1248 reads: +421 validated
umi TGCTGAGAGT = 1461 reads: +421 validated
umi TTTGCTTACT = 1964 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=653]
0-201 ==> 0-201 on |634|TRBV5-4|5'UTR| [len=201] (mis=1)
201-544 ==> 0-343 on |635|TRBV5-4|L-REGION+V-REGION| [len=343] (mis=4)
551-601 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=5)
601-653 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 185 reads
cdr3 = CASSYPEGPGDEQFF at 528, score = 8 + 8
umis assigned: [5, 47, 93, 116, 157, 166, 220, 232, 357, 369] and 17 others
of which 24 are surviving nonsolos
reads assigned: 26872
start codons at 67, 201, 323, 421, 487, 559
confident = true

TIG 2[bases=509]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=2)
398-467 ==> 0-69 on |439|TRAJ52|J-REGION| [len=69] (mis=0)
467-509 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 15 umis using 1033 reads
cdr3 = CAYRSSPNAGGTSYGKLTF at 379, score = 7 + 9
umis assigned: [124, 148, 182, 282, 314, 436, 473, 479, 757, 821] and 7 others
of which 17 are surviving nonsolos
reads assigned: 19380
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370, 401, 419
confident = true
now this is a cell
paired!

GCGATGTATTTCTGTGCTTATAGGAGTAGTCCTAATGCTGGTGGTACTAGCTATGGAAAGCTGACATTTGGACAAGGGACCATCTTGACTGTCCATCCAA <==> GAGCTGGACGACTCGGCCCTGTATCTCTGTGCCAGCAGCTACCCGGAGGGCCCCGGGGATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.909 = AGTCTTTGTCATCCCT-1

using 53 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.913 = AGTCTTTGTCGCTTTC-1

using 132 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[36, 96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.914 = AGTCTTTGTCTAAACC-1

using 295 reads

====================================================================================

graph has 195 edges initially, 4 edges after simplification

total ucounts = 30
nonsolo ucounts = 23[2^4, 3^4, 4^2, 5^2, 6, 7^2, 8^3, 10, 13^2, 18, 152]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.916 = AGTCTTTGTCTAGGTT-1

using 62 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 8[2, 3, 4, 5, 6, 8^2, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.917 = AGTCTTTGTCTCTTAT-1

using 57 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[23, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.918 = AGTCTTTGTCTGGAGA-1

using 106 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 11[2^3, 4, 6, 8^2, 9, 11, 12, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.919 = AGTCTTTGTCTTGATG-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.920 = AGTCTTTGTCTTGTCC-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.921 = AGTCTTTGTCTTTCAT-1

using 117 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.925 = AGTCTTTGTGCAGGTA-1

using 7944 reads

====================================================================================

graph has 6722 edges initially, 180 edges after simplification

total ucounts = 948
nonsolo ucounts = 685[2^110, 3^80, 4^64, 5^40, 6^46, 7^29, 8^38, 9^35, 10^28, 11^18, 12^16, 13^15, 14^12, 15^10, 16^13, 17^16, 18^12, 19^5, 20^6, 21^11, 22^3, 23^5, 24^5, 25^5, 26^4, 27, 28^3, 29^2, 30^7, 31^3, 32^5, 33^5, 34^2, 35, 36^3, 37^2, 38^4, 39, 40, 44, 46^4, 51, 52, 54^3, 55, 60, 61, 66^2, 79, 94, 111, 414]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.927 = AGTCTTTGTGCCTTGG-1

using 162 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3, 4, 5, 6^2, 8, 24, 38, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.937 = AGTCTTTGTTAGAACA-1

using 17 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.939 = AGTCTTTGTTAGTGGG-1

using 14 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.940 = AGTCTTTGTTCCAACA-1

using 58 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.943 = AGTCTTTGTTCGCGAC-1

using 78 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.947 = AGTCTTTGTTGACGTT-1

using 68 reads

====================================================================================

graph has 62 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 12, 13, 16, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.949 = AGTCTTTGTTGGAGGT-1

using 1365 reads

====================================================================================

graph has 488 edges initially, 16 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2, 4, 5, 6^2, 7, 10, 14, 17, 23, 29, 37^2, 1163]
surviving nonsolo ucounts = 1[1163]
ids = [9]

====================================================================================

UMI info for barcode AGTCTTTGTTGGAGGT-1 contig 1 = GGGTCACAGT...
umi CGCTTTTTGT = 1179 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=498]
52-399 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
398-446 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
446-498 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 72 reads
cdr3 = CASSLALETQYF at 382, score = 7 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 1149
start codons at 52, 76, 161, 260, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.950 = AGTCTTTGTTGGGACA-1

using 109 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[5, 10^2, 11^2, 17^2, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.951 = AGTCTTTGTTGTCTTT-1

using 55 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.952 = AGTCTTTGTTTACTCT-1

using 21 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.955 = AGTCTTTTCAACGCTA-1

using 12053 reads

====================================================================================

graph has 8735 edges initially, 349 edges after simplification

total ucounts = 1052
nonsolo ucounts = 803[2^107, 3^79, 4^63, 5^44, 6^42, 7^34, 8^42, 9^29, 10^25, 11^23, 12^22, 13^28, 14^19, 15^21, 16^18, 17^10, 18^16, 19^19, 20^12, 21^12, 22^6, 23^10, 24^4, 25^5, 26^6, 27^7, 28^9, 29^7, 30^2, 31^5, 32^5, 33^3, 34^5, 35^6, 36^2, 37^2, 38^2, 39^4, 40^3, 41, 42, 43^4, 44^2, 45^2, 46, 47, 48^2, 51, 53^2, 54, 55^5, 56, 58^2, 60^2, 62, 64, 65, 68, 71^2, 72, 78, 86, 88, 97, 99, 105, 117, 145, 160, 899]
surviving nonsolo ucounts = 2[160, 899]
ids = [373, 929]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.956 = AGTCTTTTCAACTCTT-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.960 = AGTCTTTTCACATACG-1

using 50 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[13, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.965 = AGTCTTTTCACGGTTA-1

using 29 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.966 = AGTCTTTTCACTTCAT-1

using 153 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[3^3, 4^2, 6^2, 7, 10, 11, 14, 21, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.968 = AGTCTTTTCAGCCTAA-1

using 24 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.969 = AGTCTTTTCAGCTCGG-1

using 71 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3, 4, 6, 10, 14, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.971 = AGTCTTTTCAGGTTCA-1

using 68 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.975 = AGTCTTTTCATGTCTT-1

using 34 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.976 = AGTCTTTTCCAAAGTC-1

using 53 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 3, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.977 = AGTCTTTTCCAACCAA-1

using 82 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.979 = AGTCTTTTCCACTCCA-1

using 56 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.980 = AGTCTTTTCCAGAAGG-1

using 14 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.983 = AGTCTTTTCCCAACGG-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.987 = AGTCTTTTCCCTTGCA-1

using 55 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1000 = AGTCTTTTCGGCGCAT-1

using 37 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 7, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1001 = AGTCTTTTCGGTGTTA-1

using 98 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1007 = AGTCTTTTCTACTTAC-1

using 193 reads

====================================================================================

graph has 128 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 14[2^3, 3, 4, 6^2, 10, 11, 13, 14, 29, 43, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1009 = AGTCTTTTCTATCCCG-1

using 68 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^4, 3^3, 4^2, 8, 10, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1010 = AGTCTTTTCTCACATT-1

using 54 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 5, 12, 13, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1011 = AGTCTTTTCTCGTTTA-1

using 1898 reads

====================================================================================

graph has 554 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 13[2^2, 3^2, 4^3, 6, 7, 14^2, 15, 1812]
surviving nonsolo ucounts = 1[1812]
ids = [18]

====================================================================================

UMI info for barcode AGTCTTTTCTCGTTTA-1 contig 1 = CTTCCCCAGG...
umi TCCATCCCCT = 1808 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=506]
0-57 ==> 144-201 on |634|TRBV5-4|5'UTR| [len=201] (mis=0)
57-400 ==> 0-343 on |635|TRBV5-4|L-REGION+V-REGION| [len=343] (mis=3)
398-410 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
406-454 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=4)
454-506 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 131 reads
cdr3 = CASSTGQGSYGYTF at 384, score = 8 + 9
umis assigned: [18]
of which 1 are surviving nonsolos
reads assigned: 1784
start codons at 57, 179, 277, 343, 412
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1013 = AGTCTTTTCTGCAGTA-1

using 80 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1015 = AGTCTTTTCTGGTGTA-1

using 21 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1017 = AGTCTTTTCTGTCCGT-1

using 87 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 15.1020 = AGTCTTTTCTTCCTTC-1

using 47 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.18
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk15-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk15-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

109.338 seconds used processing barcodes, peak mem = 0.31
