[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 832.50 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk12-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk12-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk12.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.0 = AGATCTGAGGCGATAC-1

using 25962 reads

====================================================================================

graph has 8506 edges initially, 76 edges after simplification

total ucounts = 360
nonsolo ucounts = 250[2^59, 3^29, 4^17, 5^21, 6^18, 7^6, 8^9, 9^4, 10^6, 11^7, 12^8, 13^4, 14^4, 15^10, 16^9, 17^3, 18, 19^6, 20^5, 23^2, 26, 27, 30, 34, 35, 37, 46, 48, 51, 52, 68^2, 79, 136, 660, 695, 1149, 1251, 1866, 2762, 3234, 11898]
surviving nonsolo ucounts = 8[660, 695, 1149, 1251, 1866, 2762, 3234, 11898]
ids = [199, 241, 34, 31, 259, 343, 25, 152]

====================================================================================

UMI info for barcode AGATCTGAGGCGATAC-1 contig 1 = AGATCTGAGG...
umi AATCTACGCC = 3242 reads: +397 validated
umi ACAGAGCGTT = 1137 reads: +397 validated
umi TTTAATTACC = 2762 reads: +397 validated

UMI info for barcode AGATCTGAGGCGATAC-1 contig 2 = GCCTTTGCCC...
umi AATTCTCACG = 1253 reads: +400 validated
umi GAGTTTTGGA = 669 reads: +400 validated

UMI info for barcode AGATCTGAGGCGATAC-1 contig 3 = TGGGAGTGAC...
umi CGCGTTAGTT = 9478 reads: -368X +6 -2XX +1 -1XX +2 -1XX +5 -1XX +5 -1XX +2 -3XX +2 invalidated
umi TATGTGTCCT = 1878 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=489]
50-386 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=1)
387-447 ==> 0-60 on |397|TRAJ12|J-REGION| [len=60] (mis=2)
447-489 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 483 reads
cdr3 = CAVRREDSSYKLIF at 374, score = 9 + 9
umis assigned: [25, 34, 343]
of which 3 are surviving nonsolos
reads assigned: 7008
start codons at 34, 50, 94, 294
confident = true

TIG 2[bases=495]
0-43 ==> 38-81 on |583|TRBV18|5'UTR| [len=81] (mis=0)
43-390 ==> 0-347 on |584|TRBV18|L-REGION+V-REGION| [len=347] (mis=2)
395-443 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=3)
443-495 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 106 reads
cdr3 = CASSRQGGDYGYTF at 373, score = 7 + 9
umis assigned: [31, 199]
of which 2 are surviving nonsolos
reads assigned: 1879
start codons at 28, 43, 101, 112, 165, 175, 191, 235, 266, 277, 401
confident = true

TIG 3[bases=501]
49-396 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=0)
409-449 ==> 8-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=0)
449-501 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 128 reads
cdr3 = CASSLASGIIEAFF at 379, score = 7 + 8
umis assigned: [152, 259]
of which 2 are surviving nonsolos
reads assigned: 11192
start codons at 49, 73, 158, 257, 370
confident = true

REJECT CONTIGS

TIG 1[bases=451]
1-162 ==> 912-1073 on segment before TRAV8-5 exon 3 [len=1073] (mis=0)
367-433 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
umis assigned: [241]
of which 1 are surviving nonsolos
reads assigned: 681
start codons at 24, 129, 182, 329, 366
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.6 = AGATCTGAGTACGTTC-1

using 42 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.11 = AGATCTGAGTGTCTCA-1

using 90 reads

====================================================================================

graph has 50 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 19, 21, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.13 = AGATCTGCAAAGGCGT-1

using 84 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.15 = AGATCTGCAAGTTCTG-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.16 = AGATCTGCAATAGAGT-1

using 1106 reads

====================================================================================

graph has 420 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^2, 3^2, 6, 19, 22, 32, 1014]
surviving nonsolo ucounts = 1[1014]
ids = [4]

====================================================================================

UMI info for barcode AGATCTGCAATAGAGT-1 contig 1 = TGGGGGCATG...
umi ATCGTACCCT = 1014 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=471]
7-354 ==> 0-347 on |562|TRBV11-1|L-REGION+V-REGION| [len=347] (mis=3)
360-372 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=0)
369-419 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
419-471 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CASSQGVVGQGASNEQFF at 337, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 1001
start codons at 7, 31, 149, 215, 230, 328, 377
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.20 = AGATCTGCACAGAGGT-1

using 66 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.21 = AGATCTGCACAGGCCT-1

using 55 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 3, 18, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.22 = AGATCTGCACGACGAA-1

using 125 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.25 = AGATCTGCAGACAGGT-1

using 44 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 4, 5, 7, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.27 = AGATCTGCAGATCTGT-1

using 85 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.28 = AGATCTGCAGCTGCAC-1

using 37 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.29 = AGATCTGCAGGAATCG-1

using 67 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2, 5, 7, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.34 = AGATCTGCAGTCAGAG-1

using 39219 reads

====================================================================================

graph has 12613 edges initially, 146 edges after simplification

total ucounts = 581
nonsolo ucounts = 432[2^65, 3^38, 4^31, 5^21, 6^17, 7^22, 8^20, 9^23, 10^16, 11^6, 12^14, 13^17, 14^16, 15^15, 16^8, 17^13, 18^9, 19^7, 20^8, 21^5, 22, 23^2, 24^6, 25^5, 26^4, 27, 29^3, 31^3, 33, 34, 36, 39, 40, 44, 48, 49, 55, 59, 60, 65, 66, 69, 71, 74, 106, 145, 624, 752, 823, 832, 1112, 1491, 1624, 1681, 1840, 1867, 2094, 2383, 2403, 2449, 2582, 2708, 3188, 3807]
surviving nonsolo ucounts = 18[624, 752, 823, 832, 1112, 1491, 1624, 1681, 1840, 1867, 2094, 2383, 2403, 2449, 2582, 2708, 3188, 3807]
ids = [529, 74, 307, 206, 486, 263, 413, 410, 448, 245, ...]

====================================================================================

UMI info for barcode AGATCTGCAGTCAGAG-1 contig 1 = TGGGGCTTCA...
umi AACTTAAGCC = 2602 reads: -10X +387 invalidated
umi AATGTAGTTG = 3498 reads: -355 +1 -1XX +2 -1XX +5 -1XX +2 -1XX +2 -1XX +8 -1XX +5 -4XX +7 invalidated
umi ACTAAATACG = 2186 reads: -375 +2 -1XX +2 -1XX +1 -1XX +4 -3XX +2 -2XX +1 -1XX +1 invalidated
umi AGAGTATCCA = 6 reads: -396X +1 invalidated
umi ATGAGAAGGG = 3178 reads: +397 validated
umi CGGGACCCTC = 1870 reads: +397 validated
umi CGTTTGGCCC = 2102 reads: +397 validated
umi CTACTCCGGG = 1496 reads: +397 validated
umi GCCTACGTCC = 2416 reads: +397 validated
umi GGCCACAGCT = 2698 reads: +397 validated
umi GTTAGACGAC = 1674 reads: +397 validated
umi GTTTACTTAG = 1482 reads: -377 +1 -2XX +1 -1XX +2 -4XX +2 -6XX +1 invalidated
umi TAGGGTGATT = 1847 reads: -7 +1 -2XX +387 invalidated
umi TTCAATCACG = 2451 reads: +397 validated

UMI info for barcode AGATCTGCAGTCAGAG-1 contig 2 = TGGGGAGTTT...
umi CCGCTCGCTT = 835 reads: +400 validated
umi CTTAGTAGCG = 1 reads: -386 +2 -3X +1 -3X +1 -3X +1 invalidated
umi GAACGTTCCC = 826 reads: +400 validated
umi TCTAGACCTA = 1107 reads: +374 -1 +25 non-validated
umi TGTTCGCGGC = 618 reads: +337 -1 +5 -1 +6 -1 +2 -1 +27 -4 +15 non-validated

GOOD CONTIGS

TIG 1[bases=467]
18-362 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
367-415 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=5)
415-467 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 10 umis using 1669 reads
cdr3 = CASSQDRLGETQYF at 345, score = 6 + 7
umis assigned: [8, 29, 64, 74, 112, 245, 260, 263, 349, 371] and 4 others
of which 14 are surviving nonsolos
reads assigned: 29078
start codons at 18, 76, 140, 153, 160, 168, 223, 226, 234, 336
confident = true

TIG 2[bases=681]
239-580 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
580-639 ==> 0-59 on |448|TRAJ7|J-REGION| [len=59] (mis=4)
639-681 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CAVGARYGNNRLAF at 566, score = 7 + 6
umis assigned: [206, 293, 307, 486, 529]
of which 4 are surviving nonsolos
reads assigned: 3344
start codons at 239, 361, 375, 402, 549, 585
confident = true
now this is a cell
paired!

CATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGTGGGTGCGCGATATGGGAACAACAGACTCGCTTTTGGGAAGGGGAACCAAGTGGTGGTCATACCAA <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGCCAAGACAGGCTAGGGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.36 = AGATCTGCATAACCTG-1

using 139 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.40 = AGATCTGCATGTAGTC-1

using 25 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.42 = AGATCTGCATTTGCCC-1

using 85 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.43 = AGATCTGGTAAGGGCT-1

using 46 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.46 = AGATCTGGTAGCGTGA-1

using 38 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 4, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.47 = AGATCTGGTAGCTTGT-1

using 53 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 48]
surviving nonsolo ucounts = 1[48]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.50 = AGATCTGGTCACTTCC-1

using 25 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.55 = AGATCTGGTCGTCTTC-1

using 50 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.57 = AGATCTGGTCTCCACT-1

using 84 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.58 = AGATCTGGTCTTGTCC-1

using 3070 reads

====================================================================================

graph has 2894 edges initially, 122 edges after simplification

total ucounts = 347
nonsolo ucounts = 241[2^54, 3^31, 4^17, 5^16, 6^11, 7^10, 8^9, 9^7, 10^3, 11^9, 12^8, 13^7, 14^3, 15^3, 16, 17, 18, 19, 20^4, 21^3, 22^2, 23, 24^3, 25^2, 26^2, 27^2, 28^2, 29^2, 30^2, 31^3, 33, 35, 36, 37, 41^2, 46, 47, 48, 52, 53, 55, 59^2, 62, 64, 65^2, 72, 92, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.59 = AGATCTGGTGATGTCT-1

using 50 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.61 = AGATCTGGTGCGAAAC-1

using 45 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.71 = AGATCTGTCACAACGT-1

using 167 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2^2, 3, 5, 6, 7, 8, 22, 46, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.73 = AGATCTGTCACGCATA-1

using 145 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 17, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.77 = AGATCTGTCATGGTCA-1

using 58 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.80 = AGATCTGTCCAAGTAC-1

using 63 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.81 = AGATCTGTCCACGACG-1

using 188 reads

====================================================================================

graph has 255 edges initially, 8 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^2, 6, 8^2, 60, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.82 = AGATCTGTCCACGCAG-1

using 217 reads

====================================================================================

graph has 176 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2, 4, 9, 24^2, 29, 52, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.83 = AGATCTGTCCAGAAGG-1

using 178 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^3, 8, 162]
surviving nonsolo ucounts = 1[162]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=314]
2-106 ==> 5724-5828 on segment before TRBD1 exon 1 [len=6000] (mis=0)
211-262 ==> 0-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
262-314 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 161
start codons at 203, 217
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.84 = AGATCTGTCCAGATCA-1

using 56051 reads

====================================================================================

graph has 28530 edges initially, 928 edges after simplification

total ucounts = 2286
nonsolo ucounts = 1771[2^199, 3^154, 4^100, 5^93, 6^71, 7^73, 8^72, 9^52, 10^50, 11^39, 12^53, 13^48, 14^48, 15^39, 16^33, 17^40, 18^27, 19^31, 20^30, 21^27, 22^28, 23^15, 24^25, 25^18, 26^15, 27^14, 28^14, 29^15, 30^12, 31^10, 32^9, 33^9, 34^6, 35^7, 36^8, 37^7, 38^5, 39^6, 40^10, 41^6, 42^7, 43, 44^10, 45^2, 46^2, 47^6, 48^4, 49^3, 50^3, 51, 52^3, 53^5, 54^3, 55^8, 56^2, 57^5, 58^4, 59^5, 60^8, 61^2, 62^4, 63^7, 64^2, 65^6, 66^4, 67^3, 68, 69^7, 70^3, 71^5, 72, 73^5, 74^6, 75, 76^2, 77^4, 78^2, 80, 81, 82^3, 84^3, 85^5, 86^5, 87^3, 88^3, 89, 90^3, 91, 92^2, 93^2, 94^2, 95^2, 96^2, 97^2, 99, 100^2, 101, 102^2, 103^3, 104^3, 105^4, 106^2, 107, 108, 109, 110^2, 111, 113^5, 114, 115^4, 116^2, 118^3, 123^2, 124^2, 125, 126, 128, 129, 130, 131, 132^2, 134, 136^2, 140, 149, 150, 154, 155, 173, 180, 708, 1700, 7471, 8544]
surviving nonsolo ucounts = 4[708, 1700, 7471, 8544]
ids = [855, 1150, 1234, 1833]

====================================================================================

REJECT CONTIGS

TIG 1[bases=372]
5-26 ==> 81-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
26-76 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=0)
76-147 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
195-267 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
320-372 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [855, 1150, 1234, 1259, 1833]
of which 4 are surviving nonsolos
reads assigned: 16772
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.85 = AGATCTGTCCATTCTA-1

using 243 reads

====================================================================================

graph has 164 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 3, 4, 9, 27, 28, 46, 121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.87 = AGATCTGTCCCTTGCA-1

using 6984 reads

====================================================================================

graph has 4729 edges initially, 182 edges after simplification

total ucounts = 512
nonsolo ucounts = 390[2^54, 3^33, 4^26, 5^17, 6^16, 7^15, 8^14, 9^15, 10^10, 11^16, 12^11, 13^15, 14^11, 15^8, 16^9, 17^7, 18^4, 19^5, 20^6, 21^3, 22^8, 23^3, 24^8, 26, 27^3, 28, 29^2, 30^2, 31^4, 32, 33, 34^5, 35^3, 36, 37^4, 38^2, 39^4, 40, 41, 43, 44^2, 46^2, 47^2, 50, 51, 53, 56, 58, 60^2, 61, 63, 66, 67^2, 70, 76^2, 77, 78, 79^2, 80, 81, 82^2, 87, 88^2, 89, 93, 95, 102, 107, 109, 130]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.89 = AGATCTGTCCGCGCAA-1

using 235 reads

====================================================================================

graph has 198 edges initially, 8 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2^2, 4^2, 6, 10, 18, 39, 71, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.90 = AGATCTGTCCGCTGTT-1

using 163 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3^3, 32, 36, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.91 = AGATCTGTCCGTACAA-1

using 44 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[5, 11, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.92 = AGATCTGTCCGTCAAA-1

using 4048 reads

====================================================================================

graph has 2042 edges initially, 68 edges after simplification

total ucounts = 303
nonsolo ucounts = 217[2^19, 3^16, 4^13, 5^21, 6^9, 7^16, 8^13, 9^7, 10^7, 11^10, 12^3, 13^8, 14^4, 15^4, 16^5, 17, 18^2, 19^6, 20, 21, 22, 23^2, 25, 26^4, 28^2, 30^3, 33, 34^2, 38^2, 39, 40^2, 43^3, 44, 48, 50^2, 52, 54, 56, 57, 59^2, 60, 63, 65, 66, 72, 73, 76, 77, 80, 81, 84, 85, 86, 92, 96, 97, 112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.96 = AGATCTGTCCTTGGTC-1

using 76 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.98 = AGATCTGTCGAGAGCA-1

using 60 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3, 6, 9, 15, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.101 = AGATCTGTCGCCGTGA-1

using 217 reads

====================================================================================

graph has 220 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[3, 4, 6, 9, 10, 16, 18, 20, 23, 26, 35, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.103 = AGATCTGTCGGTGTCG-1

using 55 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 12, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.105 = AGATCTGTCGTTTATC-1

using 52 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^2, 3, 5, 9, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.115 = AGATTGCAGAGAACAG-1

using 47 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.119 = AGATTGCAGATCCCAT-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^3, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.122 = AGATTGCAGCCAACAG-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.128 = AGATTGCAGCTCCCAG-1

using 121 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.130 = AGATTGCAGGAATTAC-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.132 = AGATTGCAGGACCACA-1

using 76 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.136 = AGATTGCAGGCTAGGT-1

using 91 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[17, 74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.140 = AGATTGCAGGGCTTCC-1

using 31 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.142 = AGATTGCAGTACTTGC-1

using 69 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.148 = AGATTGCAGTGCTGCC-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.149 = AGATTGCAGTGGTCCC-1

using 72 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.150 = AGATTGCAGTTAAGTG-1

using 30 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[11, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.151 = AGATTGCAGTTTAGGA-1

using 55 reads

====================================================================================

graph has 71 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.153 = AGATTGCCAAATCCGT-1

using 35608 reads

====================================================================================

graph has 11745 edges initially, 190 edges after simplification

total ucounts = 750
nonsolo ucounts = 531[2^86, 3^65, 4^62, 5^28, 6^29, 7^25, 8^25, 9^21, 10^17, 11^11, 12^17, 13^5, 14^15, 15^16, 16^11, 17^8, 18^7, 19^5, 20^5, 21^6, 22^2, 23^3, 24^6, 25^5, 26^2, 27^2, 28^9, 29^5, 30, 31^4, 32, 33^3, 34, 35^3, 36^2, 49, 76, 91, 165, 400, 533, 826, 857, 911, 936, 1228, 1357, 1850, 1938, 2216, 2828, 3303, 11182]
surviving nonsolo ucounts = 14[400, 533, 826, 857, 911, 936, 1228, 1357, 1850, 1938, 2216, 2828, 3303, 11182]
ids = [423, 178, 214, 161, 642, 255, 428, 374, 741, 53, ...]

====================================================================================

UMI info for barcode AGATTGCCAAATCCGT-1 contig 1 = GACTGCTCAG...
umi CCTTGACGCC = 954 reads: +394 validated
umi GCACTCATCT = 1363 reads: +394 validated
umi GGATCGAGTC = 404 reads: +339 -1 +15 -24 +15 non-validated
umi TGCTATTGGA = 911 reads: +355 -1 +11 -1 +1 -1 +5 -2 +17 non-validated

UMI info for barcode AGATTGCCAAATCCGT-1 contig 2 = GAAAGACGTG...
umi ACATGGTGCT = 1942 reads: +406 validated
umi CAGCCGTTGG = 477 reads: -381X +1 -1XX +1 -3XX +2 -1XX +1 -2XX +2 -2XX +2 -1XX +1 -1XX +1 -1XX +2 invalidated
umi CCCAGTACAT = 663 reads: +406 validated
umi GGCCATGCTA = 986 reads: +39 -1XX +1 -1XX +1 -2XX +361 invalidated
umi TACACCCGCG = 2204 reads: +406 validated
umi TCGTTATGAC = 2812 reads: +406 validated
umi TTTTCCGACT = 1847 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=678]
242-583 ==> 0-341 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=3)
583-636 ==> 8-61 on |427|TRAJ40|J-REGION| [len=61] (mis=0)
636-678 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 70 reads
cdr3 = CAVEGGTYKYIF at 569, score = 7 + 8
umis assigned: [255, 374, 423, 642]
of which 4 are surviving nonsolos
reads assigned: 3569
start codons at 242, 364, 378, 405, 552
confident = true

TIG 2[bases=524]
0-66 ==> 14-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
66-410 ==> 0-344 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=1)
416-428 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
422-472 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
472-524 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 690 reads
cdr3 = CASSYSTKGQGNQPQHF at 393, score = 9 + 8
umis assigned: [53, 178, 214, 428, 508, 601, 741]
of which 7 are surviving nonsolos
reads assigned: 10724
start codons at 24, 66, 124, 177, 201, 208, 216, 243, 307, 316, 448
confident = true

REJECT CONTIGS

TIG 1[bases=390]
22-290 ==> 46-314 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=0)
293-348 ==> 11-66 on |429|TRAJ42|J-REGION| [len=66] (mis=0)
348-390 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [700]
of which 1 are surviving nonsolos
reads assigned: 3245
start codons at 28, 42, 64, 277
confident = false
did not find CDR3

TIG 2[bases=624]
5-124 ==> 2457-2576 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
127-176 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
176-278 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
328-399 ==> 0-71 on segment before TRBJ2-5 exon 1 [len=71] (mis=0)
447-519 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
572-624 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [161, 366]
of which 2 are surviving nonsolos
reads assigned: 11906
start codons at 98
confident = false
did not find CDR3
now this is a cell
paired!

TCTGTGCATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGTGGAAGGAGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAA <==> TCCCAAACATCTGTGTACTTCTGTGCCAGCAGTTACTCGACCAAGGGACAGGGAAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.157 = AGATTGCCAAGCTGTT-1

using 33 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.158 = AGATTGCCAAGGTTTC-1

using 102 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.159 = AGATTGCCAATAACGA-1

using 174 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[8, 18, 32, 116]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.163 = AGATTGCCACCGAAAG-1

using 79 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[7, 30, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.168 = AGATTGCCACTAAGTC-1

using 49 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.169 = AGATTGCCACTATCTT-1

using 38 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.174 = AGATTGCCAGCTTCGG-1

using 65 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.176 = AGATTGCCAGGCTGAA-1

using 141 reads

====================================================================================

graph has 92 edges initially, 8 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[9, 21, 29, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.177 = AGATTGCCAGGGAGAG-1

using 184 reads

====================================================================================

graph has 218 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3^2, 5, 13, 15, 17, 29, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.180 = AGATTGCCATCACGTA-1

using 479 reads

====================================================================================

graph has 302 edges initially, 10 edges after simplification

total ucounts = 36
nonsolo ucounts = 29[2^3, 3^5, 4^3, 5, 6, 7, 8^2, 10, 14, 16^2, 17, 19^2, 25, 28, 37, 64, 69, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.184 = AGATTGCCATCTCGCT-1

using 73398 reads

====================================================================================

graph has 17074 edges initially, 164 edges after simplification

total ucounts = 773
nonsolo ucounts = 528[2^86, 3^54, 4^33, 5^45, 6^36, 7^24, 8^29, 9^21, 10^18, 11^14, 12^16, 13^16, 14^8, 15^12, 16^9, 17^12, 18^9, 19^7, 20^10, 21^4, 22^4, 23^4, 24^5, 25^3, 26^4, 27^2, 28^2, 29^3, 31, 32, 38, 45, 50, 56, 66, 68, 76, 79^2, 81, 111, 263, 300, 367, 442, 488, 522, 723, 737, 766, 786, 795, 982, 1038, 1212, 1225, 1355, 1386, 1655, 1672, 1798, 7999, 9425, 9584, 9799, 12896]
surviving nonsolo ucounts = 20[300, 367, 488, 766, 786, 795, 982, 1038, 1212, 1225, 1355, 1386, 1655, 1672, 1798, 7999, 9425, 9584, 9799, 12896]
ids = [246, 144, 84, 329, 701, 596, 436, 527, 323, 561, ...]

====================================================================================

UMI info for barcode AGATTGCCATCTCGCT-1 contig 1 = GTCCTGTCTC...
umi AGACTTCTGC = 1352 reads: +394 validated
umi CTTATGTTAT = 768 reads: +359 -1 +21 -8 +5 non-validated
umi GGGTCCTTGG = 987 reads: +394 validated
umi TACTATCACT = 1045 reads: +394 validated
umi TTATGTAGCT = 761 reads: +329 -1 +43 -15 +6 non-validated

UMI info for barcode AGATTGCCATCTCGCT-1 contig 2 = GCCAGGTTCA...
umi AACTCGAGCG = 1797 reads: +400 validated
umi ATAATTTACC = 1668 reads: +400 validated
umi ATGAATTGGC = 335 reads: -343 +57 non-validated
umi CACCTAGCAT = 1372 reads: +400 validated
umi CTGTTATACC = 1226 reads: +400 validated
umi TAGACTCTCC = 1626 reads: +400 validated
umi TATCGCTCCG = 1192 reads: +400 validated
umi TCCCCCGACA = 786 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=629]
0-183 ==> 25-208 on |587|TRBV19|5'UTR| [len=208] (mis=0)
183-521 ==> 0-338 on |588|TRBV19|L-REGION+V-REGION| [len=344] (mis=0)
522-534 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=1)
530-577 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
577-629 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 87 reads
cdr3 = CASRGTGRDEQYF at 510, score = 6 + 8
umis assigned: [81, 329, 436, 527, 701]
of which 5 are surviving nonsolos
reads assigned: 4828
start codons at 183, 241, 292, 328, 333, 397
confident = true

TIG 2[bases=517]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=5)
419-475 ==> 6-62 on |446|TRAJ6|J-REGION| [len=62] (mis=0)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 7 umis using 403 reads
cdr3 = CAMRGGSYIPTF at 408, score = 7 + 7
umis assigned: [13, 117, 144, 195, 323, 537, 561, 596]
of which 8 are surviving nonsolos
reads assigned: 9778
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414
confident = true

REJECT CONTIGS

TIG 1[bases=320]
15-210 ==> 5805-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
218-268 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
268-320 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [84, 98, 246, 366, 669, 715, 745]
of which 7 are surviving nonsolos
reads assigned: 49853
start codons at 9, 87, 97, 171, 226
confident = false
did not find CDR3
now this is a cell
paired!

GCTTCACAACTGGGGGACTCAGCAATGTATTTCTGTGCAATGAGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTCATCCGT <==> TCGGCCCAAAAGAACCCGACAGCTTTCTATCTCTGTGCCAGTAGGGGGACAGGGCGCGACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.189 = AGATTGCGTAAGGGAA-1

using 384 reads

====================================================================================

graph has 208 edges initially, 10 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[4^2, 5, 24, 26^2, 36, 43, 55, 59, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.192 = AGATTGCGTAGCGTGA-1

using 72 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3^2, 29, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.198 = AGATTGCGTCCCGACA-1

using 93 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 3, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.199 = AGATTGCGTCCCTACT-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.203 = AGATTGCGTCGCTTTC-1

using 20 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.204 = AGATTGCGTCTAGTCA-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.205 = AGATTGCGTCTCTTTA-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.208 = AGATTGCGTGGCAAAC-1

using 49 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.218 = AGATTGCTCAACACGT-1

using 108 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[108]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.222 = AGATTGCTCACGCATA-1

using 61 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.223 = AGATTGCTCAGTGCAT-1

using 53 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.226 = AGATTGCTCATTGCGA-1

using 38 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.228 = AGATTGCTCCACGACG-1

using 68 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.230 = AGATTGCTCCACGTTC-1

using 82 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.232 = AGATTGCTCCATGAAC-1

using 154 reads

====================================================================================

graph has 174 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 6^2, 11, 14, 17, 19, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.233 = AGATTGCTCCCATTAT-1

using 46 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.237 = AGATTGCTCCTAAGTG-1

using 59 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.239 = AGATTGCTCCTAGTGA-1

using 84 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.240 = AGATTGCTCCTTTACA-1

using 35 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.246 = AGATTGCTCTACCTGC-1

using 111 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 4, 7, 9, 17, 25, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.248 = AGATTGCTCTTGCATT-1

using 70 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^2, 3, 4, 5, 10, 13, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.252 = AGCAGCCAGAAGAAGC-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.253 = AGCAGCCAGAAGCCCA-1

using 60 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 6, 7, 14, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.257 = AGCAGCCAGACAAGCC-1

using 50 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.262 = AGCAGCCAGACTAGAT-1

using 17 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.271 = AGCAGCCAGATGAGAG-1

using 107 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.274 = AGCAGCCAGCAATCTC-1

using 81 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.278 = AGCAGCCAGCAGCCTC-1

using 32 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.279 = AGCAGCCAGCCACGTC-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.280 = AGCAGCCAGCCAGGAT-1

using 24 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.281 = AGCAGCCAGCGACGTA-1

using 2435 reads

====================================================================================

graph has 686 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[10, 2421]
surviving nonsolo ucounts = 1[2421]
ids = [5]

====================================================================================

UMI info for barcode AGCAGCCAGCGACGTA-1 contig 1 = TGGGGCTTCT...
umi TCTTTCCCCT = 2423 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=473]
21-365 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
374-421 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
421-473 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 160 reads
cdr3 = CASTSQRHRVHEQYF at 348, score = 6 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 2392
start codons at 21, 79, 143, 156, 163, 171, 226, 229, 237, 339
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.283 = AGCAGCCAGCGATCCC-1

using 16081 reads

====================================================================================

graph has 5366 edges initially, 46 edges after simplification

total ucounts = 289
nonsolo ucounts = 163[2^48, 3^22, 4^17, 5^13, 6^6, 7^5, 8^6, 9^3, 10^3, 11^2, 12^3, 13, 14, 16^2, 18^2, 19^3, 20^3, 21, 23, 24, 25, 26, 29, 31, 32, 35, 44, 53, 63, 69, 119, 898, 927, 1152, 1528, 1811, 1846, 2008, 2087, 2363]
surviving nonsolo ucounts = 9[898, 927, 1152, 1528, 1811, 1846, 2008, 2087, 2363]
ids = [174, 98, 167, 217, 178, 205, 77, 196, 204]

====================================================================================

UMI info for barcode AGCAGCCAGCGATCCC-1 contig 1 = AGAGGCCCCA...
umi CACACGTGCC = 2008 reads: +400 validated
umi CCGACTATCC = 896 reads: -306 +1 -2X +91 invalidated
umi GGCAGACCGT = 909 reads: +400 validated
umi GGCCGGCTTT = 1830 reads: +400 validated
umi GTATACCATA = 2097 reads: +400 validated
umi GTGCTGTTCC = 2359 reads: +400 validated
umi GTGGCAAGCA = 1856 reads: +400 validated
umi TAATCACCAT = 1540 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=481]
0-29 ==> 82-111 on |628|TRBV4-3|5'UTR| [len=111] (mis=0)
29-373 ==> 0-344 on |629|TRBV4-3|L-REGION+V-REGION| [len=344] (mis=3)
379-429 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=3)
429-481 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 849 reads
cdr3 = CASSPGTSFNNEQFF at 356, score = 10 + 8
umis assigned: [77, 98, 174, 178, 196, 204, 205, 217]
of which 8 are surviving nonsolos
reads assigned: 13243
start codons at 29, 83, 122, 128, 151, 179, 221, 286, 387
confident = true

REJECT CONTIGS

TIG 1[bases=399]
0-21 ==> 15-36 on |455|TRAV10|5'UTR| [len=36] (mis=0)
21-364 ==> 0-343 on |456|TRAV10|L-REGION+V-REGION| [len=343] (mis=6)
357-399 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [167]
of which 1 are surviving nonsolos
reads assigned: 1141
start codons at 21, 79, 149, 231, 283
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.284 = AGCAGCCAGCGCTCCA-1

using 20 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.285 = AGCAGCCAGCGCTTAT-1

using 86 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.288 = AGCAGCCAGCTGCGAA-1

using 55 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.291 = AGCAGCCAGGAATGGA-1

using 91 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.292 = AGCAGCCAGGACTGGT-1

using 44 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.294 = AGCAGCCAGGAGCGTT-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.297 = AGCAGCCAGGCAAAGA-1

using 65 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.298 = AGCAGCCAGGCATGGT-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.299 = AGCAGCCAGGCTAGAC-1

using 56 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.300 = AGCAGCCAGGCTAGGT-1

using 64 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.307 = AGCAGCCAGGTGCTTT-1

using 62 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.312 = AGCAGCCAGTAGCGGT-1

using 35 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 6, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.313 = AGCAGCCAGTATCTCG-1

using 30 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^3, 4, 6, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.318 = AGCAGCCAGTCTCCTC-1

using 124 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[124]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.321 = AGCAGCCAGTGCAAGC-1

using 65 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.324 = AGCAGCCAGTTAGGTA-1

using 29475 reads

====================================================================================

graph has 8979 edges initially, 138 edges after simplification

total ucounts = 647
nonsolo ucounts = 477[2^74, 3^48, 4^49, 5^35, 6^32, 7^27, 8^22, 9^18, 10^26, 11^13, 12^15, 13^10, 14^6, 15^11, 16^3, 17^8, 18^6, 19^12, 20^7, 21^3, 22^9, 23^4, 24^3, 27^2, 28^3, 29^3, 30, 31, 32, 34, 35, 36^2, 38, 41^2, 42, 47^2, 48, 54^2, 61, 66, 73, 84, 196, 322, 552, 1015, 2170, 5299, 6411, 8756]
surviving nonsolo ucounts = 7[322, 552, 1015, 2170, 5299, 6411, 8756]
ids = [550, 459, 401, 132, 38, 225, 335]

====================================================================================

UMI info for barcode AGCAGCCAGTTAGGTA-1 contig 1 = GTCCTGTCTC...
umi ACACGAGTCC = 4741 reads: -378 +2 -1XX +3 -1XX +4 -1XX +2 -1XX +3 -3XX +1 invalidated
umi CCTAAATCGC = 5677 reads: -378 +2 -1XX +3 -1XX +4 -1XX +2 -1XX +3 -3XX +1 invalidated
umi GAATGGTCTG = 7074 reads: -379 +1 -1XX +3 -1XX +4 -1XX +2 -1XX +3 -3XX +1 invalidated
umi GGCTGCTGTG = 1024 reads: +400 validated
umi TAACCCTGAT = 555 reads: +400 validated
umi TGATAGTCCC = 1 reads: -399X +1 invalidated

GOOD CONTIGS

TIG 1[bases=635]
0-183 ==> 25-208 on |587|TRBV19|5'UTR| [len=208] (mis=0)
183-520 ==> 0-337 on |588|TRBV19|L-REGION+V-REGION| [len=344] (mis=0)
530-583 ==> 0-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=3)
583-635 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 2 umis using 41 reads
cdr3 = CASTTTENYNSPLHF at 510, score = 6 + 9
umis assigned: [38, 225, 335, 401, 459, 559]
of which 5 are surviving nonsolos
reads assigned: 19034
start codons at 183, 241, 292, 328, 333, 397
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.327 = AGCAGCCAGTTTGCGT-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.329 = AGCAGCCCAAAGCAAT-1

using 77 reads

====================================================================================

graph has 52 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[34, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.343 = AGCAGCCCACAACGTT-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.344 = AGCAGCCCACAACTGT-1

using 61 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.347 = AGCAGCCCACACTGCG-1

using 36 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.348 = AGCAGCCCACAGATTC-1

using 67 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.360 = AGCAGCCCACGACGAA-1

using 27 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.363 = AGCAGCCCACGCGAAA-1

using 1667 reads

====================================================================================

graph has 1576 edges initially, 50 edges after simplification

total ucounts = 323
nonsolo ucounts = 179[2^55, 3^20, 4^19, 5^19, 6^9, 7^8, 8^5, 9^3, 10^3, 11^4, 12^3, 13^2, 14^2, 15^5, 16^2, 18, 20, 21^2, 22, 23^2, 24^3, 26, 28, 36, 38, 40^2, 43, 53, 100, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.366 = AGCAGCCCACGTTGGC-1

using 215 reads

====================================================================================

graph has 136 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2, 3, 7^2, 8, 9, 12, 32, 62, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.367 = AGCAGCCCACTCGACG-1

using 133 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.371 = AGCAGCCCACTTCTGC-1

using 2156 reads

====================================================================================

graph has 542 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[2155]
surviving nonsolo ucounts = 1[2155]
ids = [0]

====================================================================================

UMI info for barcode AGCAGCCCACTTCTGC-1 contig 1 = GGGAGATCCT...
umi CATTGTTGCT = 2147 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=467]
0-24 ==> 0-24 on |558|TRBV10-3|5'UTR| [len=24] (mis=2)
24-368 ==> 0-344 on |559|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=3)
367-415 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=0)
415-467 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 159 reads
cdr3 = CAISENQETQYF at 351, score = 10 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 2120
start codons at 24, 46, 78, 82, 146, 174, 202, 229, 265
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.372 = AGCAGCCCAGAAGCAC-1

using 125 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[125]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.378 = AGCAGCCCAGCTGTAT-1

using 34 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.380 = AGCAGCCCAGCTGTTA-1

using 56 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[7, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.381 = AGCAGCCCAGCTTAAC-1

using 1117 reads

====================================================================================

graph has 962 edges initially, 28 edges after simplification

total ucounts = 242
nonsolo ucounts = 137[2^33, 3^22, 4^12, 5^14, 6^10, 7^2, 8^8, 9^5, 10^7, 11^3, 12^2, 13^2, 14^2, 15^3, 17, 19, 20, 21, 24, 28, 32, 33, 36, 37, 38, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.383 = AGCAGCCCAGGCGATA-1

using 22 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.386 = AGCAGCCCAGGGTTAG-1

using 82 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.390 = AGCAGCCCATCCTAGA-1

using 23205 reads

====================================================================================

graph has 5830 edges initially, 102 edges after simplification

total ucounts = 560
nonsolo ucounts = 349[2^86, 3^36, 4^39, 5^25, 6^22, 7^14, 8^17, 9^6, 10^13, 11^8, 12^10, 13^8, 14^4, 15^8, 16^3, 17^6, 18^3, 19^5, 20^2, 21^2, 22, 24, 25^2, 26, 27, 29, 30, 31^2, 33, 35^4, 37, 38^2, 39, 40, 48, 49, 52, 55, 57, 62, 72, 106, 117, 4405, 7376, 7979]
surviving nonsolo ucounts = 3[4405, 7376, 7979]
ids = [71, 501, 320]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.396 = AGCAGCCCATGCGCAC-1

using 42 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.400 = AGCAGCCCATGTCTCC-1

using 1908 reads

====================================================================================

graph has 438 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3^2, 5, 6, 16, 1872]
surviving nonsolo ucounts = 1[1872]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.402 = AGCAGCCCATTAACCG-1

using 6246 reads

====================================================================================

graph has 2704 edges initially, 56 edges after simplification

total ucounts = 342
nonsolo ucounts = 195[2^41, 3^20, 4^25, 5^11, 6^11, 7^10, 8^10, 9^8, 10^2, 11^6, 12^7, 13^3, 14^4, 16^4, 17^7, 19, 20, 21, 23^5, 26, 28, 29, 31^2, 33^3, 35^2, 47, 51, 54, 61, 62, 75, 113, 4114]
surviving nonsolo ucounts = 1[4114]
ids = [326]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.403 = AGCAGCCCATTGGTAC-1

using 40 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.404 = AGCAGCCCATTTGCTT-1

using 8633 reads

====================================================================================

graph has 4217 edges initially, 82 edges after simplification

total ucounts = 444
nonsolo ucounts = 286[2^49, 3^37, 4^28, 5^24, 6^18, 7^11, 8^13, 9^10, 10^13, 11^6, 12^3, 13^8, 14^6, 15^6, 16^6, 17^6, 18^7, 19^4, 20^4, 21^5, 22^2, 23, 24, 25^2, 27^2, 28^2, 29^2, 31, 40^2, 42, 44, 45, 49, 55, 60, 5844]
surviving nonsolo ucounts = 1[5844]
ids = [123]

====================================================================================

REJECT CONTIGS

TIG 1[bases=362]
5-260 ==> 2971-3226 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
260-310 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=1)
310-362 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [123]
of which 1 are surviving nonsolos
reads assigned: 5766
start codons at 42, 119, 268
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.409 = AGCAGCCGTAAGGGAA-1

using 71 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.414 = AGCAGCCGTACTCTCC-1

using 45 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3^2, 6, 10, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.419 = AGCAGCCGTCACCCAG-1

using 40 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.420 = AGCAGCCGTCATACTG-1

using 52 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[8, 17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.421 = AGCAGCCGTCCAACTA-1

using 119 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.422 = AGCAGCCGTCCAAGTT-1

using 28 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.423 = AGCAGCCGTCCAGTTA-1

using 40 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.424 = AGCAGCCGTCCCGACA-1

using 38 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.430 = AGCAGCCGTCTTCTCG-1

using 30 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.431 = AGCAGCCGTCTTGTCC-1

using 94 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.435 = AGCAGCCGTGGGTATG-1

using 236 reads

====================================================================================

graph has 202 edges initially, 12 edges after simplification

total ucounts = 19
nonsolo ucounts = 14[2, 3, 6^2, 7^2, 8^2, 13, 14, 17, 20, 37, 83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.438 = AGCAGCCGTGTGAATA-1

using 29 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.440 = AGCAGCCGTGTTTGGT-1

using 1452 reads

====================================================================================

graph has 1264 edges initially, 50 edges after simplification

total ucounts = 256
nonsolo ucounts = 144[2^33, 3^26, 4^13, 5^9, 6^11, 7^4, 8^5, 9^3, 11^4, 12^3, 13^7, 15^2, 16^4, 17^2, 18^3, 19, 21, 24, 28^3, 30, 32^2, 41, 47, 48, 62, 70, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.442 = AGCAGCCGTTACGACT-1

using 9 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.444 = AGCAGCCGTTCAGACT-1

using 73 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.445 = AGCAGCCGTTCCAACA-1

using 19649 reads

====================================================================================

graph has 7229 edges initially, 106 edges after simplification

total ucounts = 503
nonsolo ucounts = 345[2^83, 3^37, 4^45, 5^22, 6^32, 7^15, 8^10, 9^13, 10^5, 11^9, 12^2, 13^5, 14^5, 15^6, 16, 17^4, 18^3, 19^8, 20^4, 21^2, 22, 23, 25^2, 26^2, 27^3, 31, 35, 36, 41, 44, 49, 51, 66, 70, 92, 93, 101, 205, 548, 796, 809, 1186, 1264, 1284, 1329, 1564, 1652, 1672, 2072, 2302]
surviving nonsolo ucounts = 13[205, 548, 796, 809, 1186, 1264, 1284, 1329, 1564, 1652, 1672, 2072, 2302]
ids = [432, 233, 335, 367, 172, 138, 338, 56, 141, 437, ...]

====================================================================================

UMI info for barcode AGCAGCCGTTCCAACA-1 contig 1 = ACAGTGATCC...
umi AAGGACACTC = 2084 reads: +400 validated
umi ACTCAAGCCA = 1327 reads: +400 validated
umi CACGCCATCA = 1262 reads: +400 validated
umi CACGGGCATC = 1572 reads: +400 validated
umi CCATTCGGCT = 1175 reads: +400 validated
umi GTTACTGTCA = 2297 reads: +400 validated
umi TAACAGTCCT = 726 reads: -367X +1 -3X +1 -4XX +1 -4XX +3 -4XX +1 -2XX +2 -1XX +2 -2XX +2 invalidated
umi TAACTAGGTA = 1700 reads: +400 validated
umi TAATACAACC = 1270 reads: +400 validated
umi TATTCTCTCG = 723 reads: -388 +1 -1XX +2 -1XX +2 -3XX +2 invalidated
umi TGGCCTCACA = 208 reads: +368 -21 +11 non-validated

UMI info for barcode AGCAGCCGTTCCAACA-1 contig 2 = AGATCAGAAG...
umi CGTTTACGAT = 531 reads: +407 -1 +1 -2XX +4 invalidated
umi TGGCTTTGTC = 1655 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=499]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
399-447 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=5)
447-499 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 8 umis using 800 reads
cdr3 = CASSFRDRVYGYTF at 377, score = 8 + 9
umis assigned: [18, 56, 138, 141, 172, 329, 335, 336, 338, 367] and 1 others
of which 11 are surviving nonsolos
reads assigned: 14112
start codons at 47, 156, 405
confident = true

TIG 2[bases=503]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=0)
395-461 ==> 0-66 on |413|TRAJ28|J-REGION| [len=66] (mis=5)
461-503 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 130 reads
cdr3 = CAYRSARFGAGSYQLTF at 379, score = 7 + 10
umis assigned: [233, 437]
of which 2 are surviving nonsolos
reads assigned: 2159
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true
now this is a cell
paired!

GATGCCGCGATGTATTTCTGTGCTTATAGGAGCGCGAGGTTTGGGGCTGGGAGTTACCAACTCACTTTCGGGAAGGGGACCAAACTCTCGGTCATACCAA <==> ACACAGCAGGAGGACTCAGCCGTGTATCTCTGTGCCAGCAGCTTTCGGGACAGAGTTTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.448 = AGCAGCCGTTCCGGCA-1

using 28 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.451 = AGCAGCCGTTTAAGCC-1

using 93 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.454 = AGCAGCCGTTTGACAC-1

using 82 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.455 = AGCAGCCGTTTGACTG-1

using 11 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.457 = AGCAGCCTCAACGAAA-1

using 54 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.458 = AGCAGCCTCAAGATCC-1

using 64 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[7, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.459 = AGCAGCCTCAAGGCTT-1

using 55 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.461 = AGCAGCCTCACCCGAG-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.464 = AGCAGCCTCACCTTAT-1

using 89 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 9, 22, 54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.465 = AGCAGCCTCACGCATA-1

using 24070 reads

====================================================================================

graph has 9968 edges initially, 150 edges after simplification

total ucounts = 1133
nonsolo ucounts = 738[2^159, 3^96, 4^79, 5^60, 6^39, 7^34, 8^33, 9^32, 10^18, 11^15, 12^25, 13^15, 14^10, 15^9, 16^8, 17^17, 18^6, 19^6, 20^4, 21^6, 22^3, 23^4, 24^3, 25^5, 26^4, 27, 28^3, 29^2, 30^4, 31, 33^3, 34, 35, 37^2, 38, 39^3, 42, 43^2, 44, 47^2, 48^3, 50, 53, 56^2, 57^2, 58, 63, 66, 67, 68, 85, 221, 706, 1282, 5608, 9330]
surviving nonsolo ucounts = 4[221, 706, 5608, 9330]
ids = [783, 1088, 800, 858]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.467 = AGCAGCCTCACTTACT-1

using 1230 reads

====================================================================================

graph has 466 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 4, 1219]
surviving nonsolo ucounts = 1[1219]
ids = [5]

====================================================================================

UMI info for barcode AGCAGCCTCACTTACT-1 contig 1 = GTCATCCCTC...
umi TAAGCGTCCC = 1222 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=524]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
423-472 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
472-524 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CSARGTSDSTDTQYF at 399, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1204
start codons at 21, 78, 216, 252, 258, 271, 331
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.469 = AGCAGCCTCAGGATCT-1

using 42 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.472 = AGCAGCCTCAGTCCCT-1

using 71 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 4^3, 8, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.474 = AGCAGCCTCATCACCC-1

using 76 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.475 = AGCAGCCTCATGCATG-1

using 43 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 8, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.476 = AGCAGCCTCATGTCCC-1

using 154 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 5, 6, 7, 8, 9^2, 11, 15, 23, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.480 = AGCAGCCTCCACTGGG-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.486 = AGCAGCCTCCCACTTG-1

using 56599 reads

====================================================================================

graph has 29486 edges initially, 1230 edges after simplification

total ucounts = 2013
nonsolo ucounts = 1728[2^173, 3^98, 4^69, 5^63, 6^72, 7^49, 8^45, 9^41, 10^36, 11^39, 12^34, 13^37, 14^31, 15^26, 16^30, 17^20, 18^27, 19^21, 20^30, 21^18, 22^25, 23^11, 24^20, 25^9, 26^11, 27^16, 28^21, 29^14, 30^13, 31^14, 32^11, 33^11, 34^13, 35^4, 36^19, 37^8, 38^19, 39^10, 40^11, 41^14, 42^11, 43^11, 44^10, 45^11, 46^7, 47^12, 48^15, 49^9, 50^13, 51^9, 52^4, 53^7, 54^14, 55^13, 56^8, 57^8, 58^5, 59^6, 60^10, 61^10, 62^10, 63, 64^7, 65^4, 66^5, 67^8, 68^8, 69^11, 70^8, 71^9, 72^7, 73^4, 74^4, 75^8, 76^5, 77^7, 78^9, 79^6, 80^3, 81^6, 82^10, 83^5, 84^3, 85^6, 87^4, 88^4, 89^6, 90^3, 91^7, 92^2, 93^8, 94^5, 95^8, 96^9, 97^6, 98^4, 99^4, 100^3, 101^3, 102^2, 103, 104^3, 105, 106^2, 107^3, 108^4, 109^3, 110^5, 111^3, 112^5, 114^3, 115^2, 116, 117, 118, 119^3, 120, 121, 122, 123^2, 124^2, 125, 127, 129, 130, 131, 134, 138, 139, 140^4, 141, 144, 147, 149, 151, 155, 193, 251, 1450, 1718]
surviving nonsolo ucounts = 3[108, 111, 1718]
ids = [815, 294, 29]

====================================================================================

REJECT CONTIGS

TIG 1[bases=485]
4-71 ==> 733-800 on segment before TRAJ12 exon 1 [len=800] (mis=0)
194-258 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=0)
443-485 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [29, 815, 1023, 1165, 1589, 1620, 1808]
of which 2 are surviving nonsolos
reads assigned: 1764
start codons at 17, 26, 73, 282, 328, 403
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.496 = AGCAGCCTCCTGCCAT-1

using 62 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.497 = AGCAGCCTCCTGCTTG-1

using 112 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[112]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.500 = AGCAGCCTCGATCCCT-1

using 115 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.501 = AGCAGCCTCGCCAGCA-1

using 72 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.506 = AGCAGCCTCGGTCCGA-1

using 23 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.508 = AGCAGCCTCGTCTGAA-1

using 15 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.509 = AGCAGCCTCGTCTGCT-1

using 132 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.511 = AGCAGCCTCTACTCAT-1

using 193 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3, 6, 7, 10, 75, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.513 = AGCAGCCTCTAGAGTC-1

using 24 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.514 = AGCAGCCTCTATGTGG-1

using 27 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.518 = AGCAGCCTCTGAGGGA-1

using 32 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.520 = AGCAGCCTCTGCGGCA-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.523 = AGCAGCCTCTGGAGCC-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.524 = AGCAGCCTCTGGGCCA-1

using 45 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.526 = AGCAGCCTCTGTTTGT-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.528 = AGCAGCCTCTTATCTG-1

using 74 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.530 = AGCAGCCTCTTCGAGA-1

using 86 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 12, 14, 24, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.539 = AGCATACAGACGACGT-1

using 126 reads

====================================================================================

graph has 108 edges initially, 10 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 4^2, 18, 28, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.540 = AGCATACAGACTAAGT-1

using 25 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.542 = AGCATACAGAGTACAT-1

using 149 reads

====================================================================================

graph has 134 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 9[2, 3, 9, 11, 15, 18, 25, 30, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.550 = AGCATACAGCCAGTTT-1

using 41 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.553 = AGCATACAGCGTAGTG-1

using 1490 reads

====================================================================================

graph has 552 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 1484]
surviving nonsolo ucounts = 1[1484]
ids = [1]

====================================================================================

UMI info for barcode AGCATACAGCGTAGTG-1 contig 1 = AGTGGTCACA...
umi AAAGGTTCAT = 1493 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=489]
0-49 ==> 29-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
49-384 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
389-437 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
437-489 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 109 reads
cdr3 = CSAVDLTQETQYF at 370, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 1465
start codons at 49, 187, 223, 229, 242, 302
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.557 = AGCATACAGCTGTCTA-1

using 75 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.559 = AGCATACAGGAGTTTA-1

using 6132 reads

====================================================================================

graph has 4586 edges initially, 160 edges after simplification

total ucounts = 762
nonsolo ucounts = 522[2^98, 3^56, 4^43, 5^34, 6^32, 7^25, 8^17, 9^16, 10^16, 11^18, 12^10, 13^12, 14^17, 15^16, 16^6, 17^5, 18^9, 19^7, 20^4, 21^5, 22^5, 23^12, 24^4, 25^2, 26^6, 27^4, 28^2, 29^3, 30, 31, 32^3, 33^2, 34^6, 36^3, 38, 39^3, 40^2, 41, 42, 43, 45, 46, 47, 57^2, 63, 66, 68, 80, 89, 90, 103, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.560 = AGCATACAGGCACATG-1

using 8685 reads

====================================================================================

graph has 5921 edges initially, 135 edges after simplification

total ucounts = 658
nonsolo ucounts = 493[2^68, 3^49, 4^58, 5^40, 6^30, 7^28, 8^23, 9^30, 10^16, 11^9, 12^10, 13^7, 14^12, 15^6, 16^11, 17^10, 18^7, 19^7, 20^11, 21^5, 22^3, 23^4, 24^6, 25^2, 26^3, 27^3, 28, 29^2, 30, 31^4, 32^2, 33, 34^3, 35, 37^2, 40, 43, 45^2, 49, 50, 54, 58, 59, 62, 64, 80, 87, 89, 93, 99, 435, 2806]
surviving nonsolo ucounts = 2[435, 2806]
ids = [383, 171]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.564 = AGCATACAGGCTCTTA-1

using 114 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.568 = AGCATACAGTAACCCT-1

using 786 reads

====================================================================================

graph has 266 edges initially, 6 edges after simplification

total ucounts = 29
nonsolo ucounts = 25[3, 9, 11, 12^2, 16^2, 19^2, 20, 23^2, 24^2, 26, 34^2, 38, 39, 55, 57, 64, 65, 66, 73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.569 = AGCATACAGTAAGTAC-1

using 56 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.570 = AGCATACAGTAATCCC-1

using 267 reads

====================================================================================

graph has 150 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 5, 11, 12, 14, 26, 29, 36, 45, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.574 = AGCATACAGTGAATTG-1

using 26 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.584 = AGCATACCAAGCCTAT-1

using 57 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.585 = AGCATACCAAGGTTTC-1

using 128 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[128]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.587 = AGCATACCAATCAGAA-1

using 26 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.592 = AGCATACCACAAGACG-1

using 58 reads

====================================================================================

graph has 55 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 4, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.594 = AGCATACCACCAACCG-1

using 216 reads

====================================================================================

graph has 174 edges initially, 6 edges after simplification

total ucounts = 22
nonsolo ucounts = 16[2^2, 3^2, 4^2, 5^3, 12^2, 14, 21^2, 38, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.595 = AGCATACCACCAGCAC-1

using 69 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.598 = AGCATACCACTCGACG-1

using 5680 reads

====================================================================================

graph has 5188 edges initially, 150 edges after simplification

total ucounts = 635
nonsolo ucounts = 481[2^77, 3^46, 4^51, 5^34, 6^23, 7^23, 8^16, 9^17, 10^19, 11^22, 12^15, 13^19, 14^13, 15^11, 16^13, 17^8, 18^11, 19^9, 20^6, 21^7, 22^2, 23^7, 24^2, 25^2, 26^2, 27^4, 28^2, 30, 31^2, 34, 35, 38, 43, 44, 55, 56, 61, 67, 70, 89, 91, 96, 133, 140, 206, 225]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.599 = AGCATACCAGAAGCAC-1

using 136 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 29, 37, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.606 = AGCATACCAGCTCGCA-1

using 7311 reads

====================================================================================

graph has 4018 edges initially, 88 edges after simplification

total ucounts = 482
nonsolo ucounts = 344[2^59, 3^44, 4^32, 5^29, 6^25, 7^19, 8^12, 9^18, 10^11, 11^7, 12^5, 13^13, 14^10, 15^8, 16^7, 17^5, 18, 19^3, 20^5, 21^2, 22, 23^4, 24^2, 25^4, 26, 27^2, 29^2, 31, 34, 36, 37, 41, 48^2, 53, 61, 76, 95, 119, 3940]
surviving nonsolo ucounts = 1[3940]
ids = [447]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.608 = AGCATACCAGGGTACA-1

using 36 reads

====================================================================================

graph has 30 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 14, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.610 = AGCATACCAGTAACGG-1

using 34 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.611 = AGCATACCAGTAAGCG-1

using 57 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.619 = AGCATACCATCCGCGA-1

using 806 reads

====================================================================================

graph has 537 edges initially, 14 edges after simplification

total ucounts = 25
nonsolo ucounts = 22[2^2, 3, 5, 6, 8, 17, 21, 22, 28, 32^2, 41, 48, 50, 51, 60, 65^2, 66, 85, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.620 = AGCATACCATCCGTGG-1

using 378 reads

====================================================================================

graph has 371 edges initially, 14 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2, 5, 6, 7, 11, 13, 18, 19, 21, 41, 67, 79, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.628 = AGCATACGTAAGGGCT-1

using 321 reads

====================================================================================

graph has 188 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2, 5, 8^2, 9^2, 10, 11, 17, 18, 26, 51, 144]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.631 = AGCATACGTACAGACG-1

using 70 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 8, 11, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.635 = AGCATACGTACGCACC-1

using 63 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 4^2, 8, 17, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.638 = AGCATACGTAGCGTGA-1

using 88 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.640 = AGCATACGTATCGCAT-1

using 88 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.646 = AGCATACGTCTGCGGT-1

using 22371 reads

====================================================================================

graph has 7517 edges initially, 128 edges after simplification

total ucounts = 579
nonsolo ucounts = 401[2^73, 3^54, 4^54, 5^40, 6^22, 7^16, 8^22, 9^14, 10^5, 11^8, 12^9, 13^4, 14^7, 15^6, 16^9, 17^3, 18^5, 19^2, 20^2, 22^3, 23^3, 24^2, 25, 26^2, 27, 28, 29, 31^2, 32, 35, 37, 38, 39^2, 41, 44, 50, 51, 54, 57, 61, 70^2, 79, 88, 111, 257, 308, 973, 1253, 1513, 1586, 1737, 1783, 1996, 2021, 2231, 3014]
surviving nonsolo ucounts = 12[257, 308, 973, 1253, 1513, 1586, 1737, 1783, 1996, 2021, 2231, 3014]
ids = [213, 340, 254, 470, 499, 76, 73, 85, 298, 212, ...]

====================================================================================

UMI info for barcode AGCATACGTCTGCGGT-1 contig 1 = CTTTCCCAGG...
umi AGGGACCTTT = 1743 reads: +391 validated
umi AGTAAACACC = 1598 reads: +391 validated
umi ATACTAGATC = 1780 reads: +391 validated
umi CGACTGTTGT = 2033 reads: +391 validated
umi CGATAAACCC = 258 reads: +391 validated
umi GATCGAAAGG = 2060 reads: +277 -2XX +112 invalidated
umi TATAATGCCT = 3020 reads: +391 validated
umi TATACTGTGC = 2232 reads: +391 validated
umi TGCACTCTTC = 1511 reads: +391 validated

UMI info for barcode AGCATACGTCTGCGGT-1 contig 2 = GGGAGGACAG...
umi CTCCGTGCCC = 963 reads: +400 validated
umi GGCGGTTCGT = 285 reads: -375 +5 -1XX +2 -2XX +1 -4XX +2 -4XX +2 -1XX +1 invalidated
umi TCGACTGCGG = 1256 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=501]
0-58 ==> 251-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
58-401 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=0)
406-449 ==> 7-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=0)
449-501 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 981 reads
cdr3 = CASSLGQNEQFF at 385, score = 9 + 8
umis assigned: [73, 76, 85, 212, 213, 298, 425, 426, 499]
of which 9 are surviving nonsolos
reads assigned: 15917
start codons at 58, 340, 344, 407
confident = true

TIG 2[bases=559]
117-454 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=5)
454-517 ==> 0-63 on |409|TRAJ24|J-REGION| [len=63] (mis=5)
517-559 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 73 reads
cdr3 = CVVARRTDSWGKLQF at 441, score = 7 + 7
umis assigned: [254, 340, 470]
of which 3 are surviving nonsolos
reads assigned: 2473
start codons at 19, 56, 114, 117, 214, 321, 343, 349
confident = true
now this is a cell
paired!

CTCAGTGATTCAGCCACCTACCTCTGTGTGGTCGCTCGCCGAACTGACAGCTGGGGGAAATTGCAGTTTGGAGCAGGGACCCAGGTTGTGGTCACCCCAG <==> AGCACCTTGGAGCTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTGGGGCAGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.656 = AGCATACGTGGTGTAG-1

using 6422 reads

====================================================================================

graph has 2954 edges initially, 64 edges after simplification

total ucounts = 342
nonsolo ucounts = 232[2^54, 3^24, 4^19, 5^17, 6^13, 7^14, 8^8, 9^10, 10^5, 11^7, 12^4, 13^3, 14^7, 15^4, 16, 17^4, 18^2, 19^3, 20^3, 21^2, 22^2, 24, 25^2, 28, 29, 32, 33, 34^2, 35, 37^2, 39, 42^2, 44, 48, 49, 51, 52^3, 55, 59, 78, 3910]
surviving nonsolo ucounts = 2[34, 3910]
ids = [58, 257]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.657 = AGCATACGTGTATGGG-1

using 527 reads

====================================================================================

graph has 402 edges initially, 14 edges after simplification

total ucounts = 77
nonsolo ucounts = 56[2^11, 3^6, 4^5, 5^7, 6^2, 7, 8^7, 9, 10^4, 11^2, 12, 13^3, 14, 18, 19, 32, 34, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.658 = AGCATACGTGTGCCTG-1

using 146 reads

====================================================================================

graph has 152 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 10[2^2, 5, 6, 8, 9^2, 16, 22, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.664 = AGCATACGTTAGGGTG-1

using 88 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 3^2, 4, 6, 7, 13, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.667 = AGCATACGTTCGCGAC-1

using 56 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.674 = AGCATACTCAAGGTAA-1

using 81 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.677 = AGCATACTCACTCTTA-1

using 2162 reads

====================================================================================

graph has 808 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 11[2^3, 3, 5, 7, 8, 9, 15, 33, 2069]
surviving nonsolo ucounts = 1[2069]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=535]
0-52 ==> 14-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
52-396 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=0)
424-436 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
433-483 ==> 0-50 on |547|TRBJ2-4|J-REGION| [len=50] (mis=1)
483-535 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CASSYSVHGQGSVSYE at 379, score = 9 + 4
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 2034
start codons at 10, 38, 52, 110, 163, 187, 194, 202, 229, 293, 302
confident = false
not full
frameshifted full length transcript of length 535
VJ delta = -9
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.678 = AGCATACTCAGAGGTG-1

using 100 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 18
nonsolo ucounts = 9[2^3, 5, 6, 7, 9, 15, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.680 = AGCATACTCAGTCCCT-1

using 27 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.684 = AGCATACTCATGCTCC-1

using 30 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.687 = AGCATACTCCCTAACC-1

using 74 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.689 = AGCATACTCCGCAGTG-1

using 63 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.693 = AGCATACTCCTACAGA-1

using 57 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.695 = AGCATACTCCTCCTAG-1

using 77 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.697 = AGCATACTCGACGGAA-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.698 = AGCATACTCGCCATAA-1

using 107 reads

====================================================================================

graph has 60 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[4, 13, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.699 = AGCATACTCGCCTGAG-1

using 63 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.703 = AGCATACTCGGCTTGG-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.705 = AGCATACTCGTATCAG-1

using 502 reads

====================================================================================

graph has 362 edges initially, 14 edges after simplification

total ucounts = 22
nonsolo ucounts = 18[3, 4^2, 5, 6, 11, 13^2, 19^2, 29, 32, 40, 41, 43, 64, 68, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.706 = AGCATACTCTACTATC-1

using 82 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[12, 15, 26, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.707 = AGCATACTCTAGCACA-1

using 68 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 11, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.709 = AGCATACTCTATCGCC-1

using 140 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 57, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.710 = AGCATACTCTCCTATA-1

using 62 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.711 = AGCATACTCTCGCATC-1

using 46 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 5, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.716 = AGCATACTCTTGTACT-1

using 24 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.719 = AGCCTAAAGAAGGGTA-1

using 24 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.723 = AGCCTAAAGAGACTAT-1

using 23 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.725 = AGCCTAAAGAGGACGG-1

using 46 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5, 15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.726 = AGCCTAAAGAGGTACC-1

using 72 reads

====================================================================================

graph has 105 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[5, 6, 7, 11^2, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.728 = AGCCTAAAGAGTCGGT-1

using 92 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.729 = AGCCTAAAGATAGCAT-1

using 16 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.730 = AGCCTAAAGATGAGAG-1

using 11927 reads

====================================================================================

graph has 6563 edges initially, 276 edges after simplification

total ucounts = 678
nonsolo ucounts = 486[2^76, 3^47, 4^28, 5^35, 6^19, 7^16, 8^12, 9^17, 10^10, 11^11, 12^10, 13^13, 14^19, 15^19, 16^9, 17^10, 18^8, 19^5, 20^3, 21^9, 22^3, 23^7, 24^6, 25^2, 26, 27^6, 28^4, 29^4, 30^5, 31^3, 33^4, 34, 35^2, 36^3, 37^6, 38^3, 39^3, 40^2, 41^2, 42^3, 43^2, 44^2, 47^3, 48^2, 49, 51, 52^2, 55, 56, 57, 58^2, 60^2, 63^3, 64, 65, 66, 68, 70, 78, 81, 82, 85, 87, 93, 95, 100, 104, 135, 373, 3798]
surviving nonsolo ucounts = 6[2, 3, 15, 35, 373, 3798]
ids = [106, 142, 671, 58, 345, 340]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.732 = AGCCTAAAGCACCGTC-1

using 35 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.737 = AGCCTAAAGCGGATCA-1

using 43 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.740 = AGCCTAAAGCTCCCAG-1

using 80 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.744 = AGCCTAAAGGCAAAGA-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.751 = AGCCTAAAGTACACCT-1

using 30 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.753 = AGCCTAAAGTAGTGCG-1

using 62 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.755 = AGCCTAAAGTCACGCC-1

using 98 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 5, 11, 12, 66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.757 = AGCCTAAAGTGACTCT-1

using 32 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.759 = AGCCTAAAGTGCGATG-1

using 22193 reads

====================================================================================

graph has 10615 edges initially, 255 edges after simplification

total ucounts = 818
nonsolo ucounts = 601[2^90, 3^57, 4^57, 5^39, 6^35, 7^23, 8^21, 9^26, 10^18, 11^18, 12^19, 13^18, 14^17, 15^19, 16^11, 17^8, 18^11, 19^8, 20^9, 21^7, 22^11, 23^10, 24^5, 25^7, 26^6, 27^5, 28, 29^4, 30^4, 31^4, 32^4, 34, 36, 37, 39, 42^2, 44, 45, 47, 49, 53, 59, 60, 63, 65, 68^2, 69^3, 101, 103, 509, 839, 925, 1460, 1934, 3468, 6106]
surviving nonsolo ucounts = 9[2, 11, 509, 839, 925, 1460, 1934, 3468, 6106]
ids = [291, 324, 538, 644, 484, 357, 32, 80, 799]

====================================================================================

REJECT CONTIGS

TIG 1[bases=506]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
39-60 ==> 1110-1131 on segment before TRBV6-2 exon 1 [len=3166] (mis=1)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
391-407 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
405-454 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
454-506 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [32, 80, 357, 484, 538, 644, 799]
of which 7 are surviving nonsolos
reads assigned: 13321
start codons at 47, 156
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.761 = AGCCTAAAGTTAAGTG-1

using 88 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.766 = AGCCTAACAAAGCAAT-1

using 47 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.768 = AGCCTAACAAATTGCC-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.769 = AGCCTAACAACACCTA-1

using 14133 reads

====================================================================================

graph has 13041 edges initially, 544 edges after simplification

total ucounts = 1439
nonsolo ucounts = 1070[2^136, 3^99, 4^96, 5^68, 6^54, 7^52, 8^55, 9^35, 10^36, 11^40, 12^34, 13^24, 14^26, 15^28, 16^14, 17^12, 18^19, 19^25, 20^18, 21^10, 22^12, 23^13, 24^8, 25^16, 26^14, 27^11, 28^11, 29^7, 30^6, 31^6, 32^5, 33^7, 34^6, 35^6, 36^3, 37^2, 38^3, 39^3, 40^4, 41^2, 42^2, 43^3, 44^3, 45^3, 46, 48^3, 50, 51, 52^2, 53^2, 55, 59, 60, 61, 62^2, 65, 67, 68, 69, 70, 71, 76, 77, 78^2, 81, 88, 97, 98, 99, 111, 123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.770 = AGCCTAACAACGCACC-1

using 134 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2, 3^2, 4, 7, 9^2, 11, 26, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.772 = AGCCTAACAAGTTGTC-1

using 62 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 12, 17, 28]
surviving nonsolo ucounts = 1[17]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.773 = AGCCTAACAATACGCT-1

using 11 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.776 = AGCCTAACAATGAAAC-1

using 14 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.778 = AGCCTAACACAGACTT-1

using 57 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.779 = AGCCTAACACAGGAGT-1

using 59 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.785 = AGCCTAACACGGCCAT-1

using 77 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.787 = AGCCTAACACTCAGGC-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.791 = AGCCTAACAGAAGCAC-1

using 46 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.794 = AGCCTAACAGCTGTGC-1

using 44 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.797 = AGCCTAACAGTAACGG-1

using 16681 reads

====================================================================================

graph has 7465 edges initially, 168 edges after simplification

total ucounts = 702
nonsolo ucounts = 476[2^75, 3^75, 4^48, 5^35, 6^24, 7^31, 8^22, 9^12, 10^13, 11^17, 12^13, 13^10, 14^8, 15^8, 16^6, 17^7, 18^5, 19^2, 20^3, 21^6, 22^6, 23^4, 24^4, 25^6, 26^3, 27, 28, 29^3, 30^2, 31^2, 32^2, 33, 34^2, 35, 37, 38^2, 40, 45, 46^3, 53, 70, 74, 108, 133, 154, 2057, 2679, 2835, 4193]
surviving nonsolo ucounts = 6[133, 154, 2057, 2679, 2835, 4193]
ids = [628, 298, 59, 320, 264, 157]

====================================================================================

UMI info for barcode AGCCTAACAGTAACGG-1 contig 1 = AGAGGCCCCA...
umi ACGCCCGTAT = 2060 reads: +394 validated
umi CAAGTAGGTG = 4211 reads: -32X +362 invalidated
umi CTTGGGGGGC = 2699 reads: +394 validated
umi TGTCTGATCA = 102 reads: -385X +7 -1XX +1 invalidated

GOOD CONTIGS

TIG 1[bases=475]
0-29 ==> 0-29 on |622|TRBV4-1|5'UTR| [len=29] (mis=0)
29-373 ==> 0-344 on |623|TRBV4-1|L-REGION+V-REGION| [len=344] (mis=2)
382-423 ==> 8-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
423-475 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 667 reads
cdr3 = CASSQGLGGTQYF at 356, score = 10 + 8
umis assigned: [59, 157, 320, 628]
of which 4 are surviving nonsolos
reads assigned: 8893
start codons at 29, 122, 128, 151, 164, 179, 221, 237, 255, 286
confident = true

REJECT CONTIGS

TIG 1[bases=500]
2-341 ==> 1512-1851 on segment before TRAJ39 exon 1 [len=1851] (mis=0)
341-404 ==> 0-63 on |425|TRAJ39|J-REGION| [len=63] (mis=0)
458-500 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [264, 298]
of which 2 are surviving nonsolos
reads assigned: 2798
start codons at 142, 350, 361, 391
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.799 = AGCCTAACAGTTCCCT-1

using 56 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.802 = AGCCTAACATCCTAGA-1

using 16259 reads

====================================================================================

graph has 4852 edges initially, 48 edges after simplification

total ucounts = 206
nonsolo ucounts = 156[2^28, 3^17, 4^14, 5^12, 6^5, 7^6, 8^8, 9^5, 10, 11^5, 12^2, 13^5, 14, 15, 16^5, 17^3, 18^6, 19^4, 20, 21^3, 22, 23^2, 24^2, 26^2, 28, 29^2, 34, 40, 41, 42, 44, 49, 56^2, 65, 83, 784, 876, 995, 11805]
surviving nonsolo ucounts = 4[784, 876, 995, 11805]
ids = [14, 17, 52, 125]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.804 = AGCCTAACATGGATGG-1

using 149 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[5, 6, 7, 18, 40, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.806 = AGCCTAACATGTCCTC-1

using 55 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.807 = AGCCTAACATGTTGAC-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.811 = AGCCTAACATTGGGCC-1

using 57 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 11, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.817 = AGCCTAAGTAGCCTAT-1

using 7528 reads

====================================================================================

graph has 1660 edges initially, 8 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2, 3^2, 4, 5, 12, 18, 32, 42, 7401]
surviving nonsolo ucounts = 1[7401]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.819 = AGCCTAAGTAGCTAAA-1

using 17 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.820 = AGCCTAAGTAGTACCT-1

using 109 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.832 = AGCCTAAGTCTCCACT-1

using 146 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[146]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.834 = AGCCTAAGTGAGGGTT-1

using 38 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 4^2, 6, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.847 = AGCCTAAGTTCGTCTC-1

using 4021 reads

====================================================================================

graph has 3698 edges initially, 138 edges after simplification

total ucounts = 478
nonsolo ucounts = 377[2^56, 3^36, 4^30, 5^31, 6^25, 7^16, 8^14, 9^16, 10^19, 11^13, 12^16, 13^7, 14^9, 15^6, 16^14, 17^11, 18^2, 19^4, 20^6, 21^5, 22^6, 23^5, 24^3, 25^3, 26^4, 27^2, 28^3, 31, 32, 33^2, 35, 38, 39^3, 41, 51, 55, 56, 70, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.849 = AGCCTAAGTTCTGTTT-1

using 10735 reads

====================================================================================

graph has 6801 edges initially, 172 edges after simplification

total ucounts = 687
nonsolo ucounts = 529[2^66, 3^58, 4^47, 5^37, 6^27, 7^34, 8^21, 9^21, 10^16, 11^18, 12^16, 13^17, 14^15, 15^8, 16^14, 17^10, 18^8, 19^9, 20^12, 21^10, 22^5, 23^6, 24^6, 25^5, 26^4, 27, 29^3, 30^2, 31^3, 32^2, 33^2, 34, 36^3, 37, 38, 39^2, 40, 41^2, 42, 43, 46, 50, 53^2, 58, 62, 73, 75, 80, 143, 1395, 1464, 1809]
surviving nonsolo ucounts = 4[21, 1395, 1464, 1809]
ids = [583, 208, 532, 672]

====================================================================================

UMI info for barcode AGCCTAAGTTCTGTTT-1 contig 1 = GACTAGGGAT...
umi TCCGCCTACA = 1477 reads: +403 validated
umi TTTCCCTGGA = 1832 reads: +403 validated

UMI info for barcode AGCCTAAGTTCTGTTT-1 contig 2 = GGTTCCCCGA...
umi CCCGTCATGG = 1268 reads: +400 validated
umi TGCGATCACC = 14 reads: -22 +265 -113 non-validated

GOOD CONTIGS

TIG 1[bases=536]
0-91 ==> 56-147 on |470|TRAV17|5'UTR| [len=147] (mis=0)
91-428 ==> 0-337 on |471|TRAV17|L-REGION+V-REGION| [len=337] (mis=2)
428-494 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=5)
494-536 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 161 reads
cdr3 = CATDSSSGGSNYKLTF at 415, score = 6 + 9
umis assigned: [532, 672]
of which 2 are surviving nonsolos
reads assigned: 3244
start codons at 91, 126, 203, 211, 305
confident = true

TIG 2[bases=476]
0-68 ==> 13-81 on |585|TRBV18|5'UTR| [len=81] (mis=0)
68-415 ==> 0-347 on |586|TRBV18|L-REGION+V-REGION| [len=347] (mis=1)
427-468 ==> 9-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=1)
junction support: 1 umis using 64 reads
cdr3 = CASSPLAGLAEQFF at 398, score = 7 + 7
umis assigned: [208, 583]
of which 2 are surviving nonsolos
reads assigned: 1265
start codons at 53, 68, 126, 137, 190, 200, 216, 260, 291, 302
confident = true
now this is a cell
paired!

GCAGACACTGCTTCTTACTTCTGTGCTACGGATTCCTCCAGTGGAGGTAGCAACTATAAACTGACATTTGGAAAAGGAACTCTCTTAACCGTGAATCCAA <==> GTAGTGCGAGGAGATTCGGCAGCTTATTTCTGTGCCAGCTCACCACTAGCGGGACTAGCTGAGCAGTTCTTCGGGCCAGGGATACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.853 = AGCCTAATCAACACCA-1

using 64 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 4^2, 5, 7, 10, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.855 = AGCCTAATCAACGGGA-1

using 41 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.857 = AGCCTAATCAGCAACT-1

using 48 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 9, 16, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.859 = AGCCTAATCAGCTCTC-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.861 = AGCCTAATCATCTGCC-1

using 28 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.862 = AGCCTAATCATGTGGT-1

using 80 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.864 = AGCCTAATCATTCACT-1

using 29 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.868 = AGCCTAATCCACTGGG-1

using 89 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.870 = AGCCTAATCCATTCTA-1

using 18 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.871 = AGCCTAATCCCAGGTG-1

using 518 reads

====================================================================================

graph has 330 edges initially, 18 edges after simplification

total ucounts = 25
nonsolo ucounts = 23[2^5, 3, 4^3, 7, 12, 13, 16, 19, 21, 32, 33^2, 36, 47, 65^2, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.872 = AGCCTAATCCCTTGCA-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.874 = AGCCTAATCCTCAATT-1

using 55 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.877 = AGCCTAATCCTGCCAT-1

using 53 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2, 4^2, 10, 11, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.878 = AGCCTAATCCTTGCCA-1

using 18 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.884 = AGCCTAATCGGCTACG-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.887 = AGCCTAATCGTCGTTC-1

using 130 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3, 4, 7, 17, 39, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.889 = AGCCTAATCGTTTGCC-1

using 23 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.891 = AGCCTAATCTAACTTC-1

using 63 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.894 = AGCCTAATCTCAAACG-1

using 269 reads

====================================================================================

graph has 295 edges initially, 10 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2, 3, 5, 7, 9, 14, 15, 16, 28, 39, 50, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.896 = AGCCTAATCTGATACG-1

using 44 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 7, 10, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.897 = AGCCTAATCTGCAGTA-1

using 62 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.900 = AGCCTAATCTGGGCCA-1

using 58 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.902 = AGCCTAATCTTCGAGA-1

using 96 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.903 = AGCCTAATCTTGTCAT-1

using 60 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2^2, 5^2, 6, 7, 9^2, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.907 = AGCGGTCAGACGCTTT-1

using 92 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 17, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.909 = AGCGGTCAGACTTTCG-1

using 424 reads

====================================================================================

graph has 407 edges initially, 10 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^2, 3^2, 4, 5^3, 16, 72, 97^2, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.910 = AGCGGTCAGAGCTTCT-1

using 58 reads

====================================================================================

graph has 101 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 5[2^2, 5, 8, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.917 = AGCGGTCAGATGGCGT-1

using 56 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[14, 19, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.919 = AGCGGTCAGCACACAG-1

using 106 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.920 = AGCGGTCAGCAGCCTC-1

using 60 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 11, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.922 = AGCGGTCAGCATGGCA-1

using 144 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 6, 32, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.929 = AGCGGTCAGCTGATAA-1

using 26 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 6, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.931 = AGCGGTCAGGACCACA-1

using 653 reads

====================================================================================

graph has 711 edges initially, 42 edges after simplification

total ucounts = 37
nonsolo ucounts = 26[2^6, 3^5, 7, 10, 14, 18, 19, 28, 34, 37, 38, 55, 66^2, 69, 75, 79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.938 = AGCGGTCAGTACGTAA-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.940 = AGCGGTCAGTATTGGA-1

using 404 reads

====================================================================================

graph has 230 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2, 4^2, 5, 9, 60, 86, 101, 125]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.944 = AGCGGTCAGTCCTCCT-1

using 100 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.952 = AGCGGTCCAACGATCT-1

using 41 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.953 = AGCGGTCCAAGAGGCT-1

using 305 reads

====================================================================================

graph has 247 edges initially, 6 edges after simplification

total ucounts = 26
nonsolo ucounts = 12[3, 4, 5^2, 7^2, 14, 18, 20, 29, 47, 132]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.957 = AGCGGTCCAATCGGTT-1

using 36 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2, 3^2, 9, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.959 = AGCGGTCCAATGCCAT-1

using 16 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.961 = AGCGGTCCAATGGTCT-1

using 31 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.964 = AGCGGTCCACACCGCA-1

using 96 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.983 = AGCGGTCCATACAGCT-1

using 45 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[8, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.987 = AGCGGTCCATCGGACC-1

using 1816 reads

====================================================================================

graph has 698 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[11, 1799]
surviving nonsolo ucounts = 1[1799]
ids = [5]

====================================================================================

UMI info for barcode AGCGGTCCATCGGACC-1 contig 1 = GCAAGTCTTT...
umi CGCAGTGTCG = 1807 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=539]
0-109 ==> 100-209 on |489|TRAV26-1|5'UTR| [len=209] (mis=0)
109-437 ==> 0-328 on |490|TRAV26-1|L-REGION+V-REGION| [len=328] (mis=4)
434-497 ==> 0-63 on |401|TRAJ17|J-REGION| [len=63] (mis=0)
497-539 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 70 reads
cdr3 = CIVIMIKAAGNKLTF at 421, score = 8 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 1773
start codons at 109, 161, 187, 248, 254, 305, 320, 329, 334, 433
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.988 = AGCGGTCCATCGGTTA-1

using 79 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.990 = AGCGGTCCATGTCCTC-1

using 69 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.991 = AGCGGTCCATGTCTCC-1

using 456 reads

====================================================================================

graph has 260 edges initially, 20 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2, 4, 5, 7, 9, 21, 23, 31, 36, 41^2, 42, 48, 65, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.993 = AGCGGTCCATTGGTAC-1

using 46 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[8, 15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.998 = AGCGGTCGTACCGTAT-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1000 = AGCGGTCGTACTTCTT-1

using 42 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1009 = AGCGGTCGTCCAACTA-1

using 62 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1011 = AGCGGTCGTCTCCCTA-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1012 = AGCGGTCGTCTGATTG-1

using 46 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1015 = AGCGGTCGTGAGTATA-1

using 1976 reads

====================================================================================

graph has 228 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^3, 3, 4, 288, 1668]
surviving nonsolo ucounts = 2[288, 1668]
ids = [4, 11]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1021 = AGCGGTCGTTACGACT-1

using 98 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1025 = AGCGGTCGTTCCACTC-1

using 511 reads

====================================================================================

graph has 368 edges initially, 16 edges after simplification

total ucounts = 23
nonsolo ucounts = 21[2^4, 3, 4^2, 6, 8, 12, 13, 15, 19, 21, 23, 33, 44, 48, 81, 83, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1027 = AGCGGTCTCAAACAAG-1

using 28 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1030 = AGCGGTCTCACAACGT-1

using 32 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1031 = AGCGGTCTCACAATGC-1

using 91 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1032 = AGCGGTCTCACAGGCC-1

using 546 reads

====================================================================================

graph has 498 edges initially, 12 edges after simplification

total ucounts = 37
nonsolo ucounts = 28[2^3, 3^3, 6^2, 10^2, 11^2, 12, 14^3, 15, 18^2, 19, 23^2, 24, 27, 29, 60, 67, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1035 = AGCGGTCTCACTCCTG-1

using 4755 reads

====================================================================================

graph has 4791 edges initially, 116 edges after simplification

total ucounts = 806
nonsolo ucounts = 523[2^107, 3^75, 4^56, 5^53, 6^31, 7^23, 8^17, 9^11, 10^13, 11^17, 12^17, 13^15, 14^11, 15^11, 16^11, 17^4, 18^5, 19^5, 20^2, 21^2, 22^5, 23^2, 24^2, 25, 28^3, 30, 31, 32, 33^2, 35, 36^2, 38, 43, 44^2, 45, 46^2, 47, 48, 52, 53, 54^2, 56, 78, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1037 = AGCGGTCTCACTTATC-1

using 69 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1038 = AGCGGTCTCAGCGACC-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1046 = AGCGGTCTCCCTTGTG-1

using 572 reads

====================================================================================

graph has 236 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 15, 18, 46, 489]
surviving nonsolo ucounts = 1[489]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1047 = AGCGGTCTCCGAATGT-1

using 40 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1048 = AGCGGTCTCCGCGTTT-1

using 153 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 4, 5, 8, 12, 22, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1049 = AGCGGTCTCCGTTGTC-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1054 = AGCGGTCTCGCCAAAT-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[9, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1059 = AGCGGTCTCGGCCGAT-1

using 63 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1063 = AGCGGTCTCGTTACGA-1

using 1811 reads

====================================================================================

graph has 522 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 3, 7, 11, 50, 1735]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1065 = AGCGGTCTCGTTTAGG-1

using 18920 reads

====================================================================================

graph has 6622 edges initially, 80 edges after simplification

total ucounts = 493
nonsolo ucounts = 346[2^56, 3^38, 4^32, 5^24, 6^25, 7^19, 8^14, 9^14, 10^8, 11^13, 12^14, 13^11, 14^5, 15^2, 16^11, 17^3, 18^3, 19^2, 20^5, 21^4, 22^3, 23, 24, 27^2, 28, 29^2, 30^4, 32^2, 34, 35, 39, 41, 42, 43, 44^2, 47, 55, 60, 82, 85^2, 91, 92, 112, 115, 642, 731, 1458, 1611, 1890, 1925, 2125, 2131, 2545]
surviving nonsolo ucounts = 11[85, 112, 642, 731, 1458, 1611, 1890, 1925, 2125, 2131, 2545]
ids = [237, 163, 390, 395, 51, 380, 92, 75, 207, 373, ...]

====================================================================================

UMI info for barcode AGCGGTCTCGTTTAGG-1 contig 1 = ATATGTAAAA...
umi AGAAATGCCT = 1466 reads: +415 validated
umi CCGGTGACAT = 115 reads: +396 -1 +18 non-validated
umi GACATTGCTA = 83 reads: +352 -44 +19 non-validated

UMI info for barcode AGCGGTCTCGTTTAGG-1 contig 2 = ATTCTTTCTT...
umi AGTTTATCAA = 1911 reads: +397 validated
umi ATGTCCATCA = 1894 reads: -18X +379 invalidated
umi CCGTCTTCAT = 2555 reads: +397 validated
umi CTCCGTGTGT = 2141 reads: +397 validated
umi TATGAAGCCT = 2137 reads: +397 validated
umi TATTCCACCG = 1617 reads: +397 validated
umi TCAGTTACCC = 166 reads: +274 -19 +1 -1 +1 -2 +1 -2 +5 -2 +2 -1 +3 -1 +1 -1 +3 -2 +4 -1 +1 -1 +7 -1 +3 -1 +6 -45 +5 non-validated
umi TCCATCTGCC = 714 reads: -289 +3 -1XX +104 invalidated

GOOD CONTIGS

TIG 1[bases=568]
0-111 ==> 0-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=0)
111-469 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=4)
470-526 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=0)
526-568 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 87 reads
cdr3 = CAAAGNTGNQFYF at 456, score = 9 + 7
umis assigned: [51, 163, 237]
of which 3 are surviving nonsolos
reads assigned: 1634
start codons at 2, 9, 30, 111, 117, 184, 187, 273, 290, 358, 364
confident = true

TIG 2[bases=469]
0-20 ==> 0-20 on |612|TRBV28|5'UTR| [len=20] (mis=0)
20-364 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
357-369 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
367-417 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
417-469 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 848 reads
cdr3 = CASRQGGRYNEQFF at 347, score = 6 + 8
umis assigned: [75, 92, 171, 207, 373, 380, 390, 395]
of which 8 are surviving nonsolos
reads assigned: 12931
start codons at 20, 78, 142, 155, 162, 170, 225, 228, 236, 338, 375
confident = true
now this is a cell
paired!

TCCCAGCCTGGAGACTCTGCAGTGTACTTCTGTGCAGCCGCTGGGAACACCGGTAACCAGTTCTATTTTGGGACAGGGACAAGTTTGACGGTCATTCCAA <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGGCAGGGGGGGCGTTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1069 = AGCGGTCTCTAAGCCA-1

using 72 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1070 = AGCGGTCTCTCCGGTT-1

using 83 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1077 = AGCGGTCTCTGCTTGC-1

using 98 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1078 = AGCGGTCTCTGGTATG-1

using 7731 reads

====================================================================================

graph has 1298 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 8, 7717]
surviving nonsolo ucounts = 1[7717]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1082 = AGCGGTCTCTTACCGC-1

using 209 reads

====================================================================================

graph has 140 edges initially, 10 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2^3, 15, 34, 50, 104]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1084 = AGCGGTCTCTTCCTTC-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1086 = AGCGGTCTCTTGAGAC-1

using 711 reads

====================================================================================

graph has 580 edges initially, 20 edges after simplification

total ucounts = 39
nonsolo ucounts = 29[2^4, 3^4, 4^2, 5^2, 8^2, 11, 12, 13, 14, 19, 22, 24, 25, 31, 40, 52, 55, 76, 96, 157]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1089 = AGCGTATAGAAGATTC-1

using 66 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1093 = AGCGTATAGAGGTAGA-1

using 61 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[2^2, 8^2, 20, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1095 = AGCGTATAGATCGATA-1

using 23 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1098 = AGCGTATAGCACGCCT-1

using 66 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1102 = AGCGTATAGCGGCTTC-1

using 16 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1105 = AGCGTATAGGAATTAC-1

using 27123 reads

====================================================================================

graph has 11435 edges initially, 269 edges after simplification

total ucounts = 869
nonsolo ucounts = 637[2^88, 3^54, 4^42, 5^32, 6^34, 7^27, 8^36, 9^23, 10^16, 11^31, 12^14, 13^10, 14^10, 15^12, 16^15, 17^12, 18^13, 19^9, 20^14, 21^15, 22^10, 23^7, 24^10, 25^6, 26^4, 27^7, 28^12, 29^4, 30^10, 31^2, 32^5, 33^6, 34^3, 35^2, 36, 37, 38^3, 39^5, 42^3, 43^2, 46^2, 47, 50^2, 55, 56, 59, 65, 68, 78, 107, 152, 168, 550, 600, 612, 651, 826, 1067, 1145, 1188, 1963, 1978, 2340, 2401, 3403]
surviving nonsolo ucounts = 15[152, 168, 550, 600, 612, 651, 826, 1067, 1145, 1188, 1963, 1978, 2340, 2401, 3403]
ids = [31, 637, 499, 838, 389, 119, 329, 587, 357, 69, ...]

====================================================================================

UMI info for barcode AGCGTATAGGAATTAC-1 contig 1 = TTTGGGGATG...
umi AACTACACAT = 152 reads: +391 validated
umi ACTCCCCCTT = 650 reads: +391 validated
umi AGCGAATCCA = 1985 reads: +391 validated
umi CCGGCTCCGA = 738 reads: +391 validated
umi CTGAGAAGCT = 1982 reads: +391 validated
umi GCCCCCACCT = 513 reads: -370 +1 -1XX +1 -3XX +4 -1XX +8 -1XX +1 invalidated
umi GTCTGTTTTA = 1073 reads: +391 validated
umi TACATTTAGG = 169 reads: +391 validated
umi TATCCCTCTA = 2354 reads: +391 validated
umi TTGCCCCAAT = 606 reads: +391 validated

UMI info for barcode AGCGTATAGGAATTAC-1 contig 2 = ACAGAAGTGG...
umi ACAATCCGAT = 1190 reads: +397 validated
umi AGTGCGATGG = 3422 reads: -12 +1 -1XX +383 invalidated
umi CGCTACAGGC = 1152 reads: +397 validated
umi CTACGCTCCA = 611 reads: +397 validated
umi CTCGTTTGAT = 2413 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=502]
0-59 ==> 0-59 on |567|TRBV12-4|5'UTR| [len=59] (mis=3)
59-406 ==> 0-347 on |568|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=4)
400-450 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=4)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 611 reads
cdr3 = CASSYSNEQFF at 389, score = 8 + 8
umis assigned: [31, 119, 145, 329, 417, 499, 587, 637, 679, 838]
of which 10 are surviving nonsolos
reads assigned: 10058
start codons at 7, 59, 117, 158, 181, 230, 233, 282, 293, 320, 327, 408
confident = true

TIG 2[bases=508]
0-69 ==> 0-69 on |478|TRAV20|5'UTR| [len=69] (mis=0)
69-406 ==> 0-337 on |479|TRAV20|L-REGION+V-REGION| [len=337] (mis=0)
403-466 ==> 0-63 on |426|TRAJ4|J-REGION| [len=63] (mis=5)
466-508 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 562 reads
cdr3 = CAVQAPGGYNKLIF at 393, score = 8 + 9
umis assigned: [69, 163, 357, 389, 408]
of which 5 are surviving nonsolos
reads assigned: 8644
start codons at 69, 78
confident = true
now this is a cell
paired!

AAACCTGAAGACTCAGCCACTTATCTCTGTGCTGTGCAGGCACCTGGTGGCTACAATAAGCTGATTTTTGGAGCAGGGACCAGGCTGGCTGTACACCCAT <==> ATCCAGCCCTCAGAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGTTACAGCAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1106 = AGCGTATAGGATATAC-1

using 87 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 7, 8^2, 15, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1107 = AGCGTATAGGATGTAT-1

using 22816 reads

====================================================================================

graph has 13272 edges initially, 392 edges after simplification

total ucounts = 1310
nonsolo ucounts = 1018[2^122, 3^113, 4^89, 5^73, 6^59, 7^46, 8^41, 9^40, 10^34, 11^39, 12^30, 13^23, 14^28, 15^32, 16^20, 17^23, 18^24, 19^14, 20^24, 21^10, 22^15, 23^17, 24^11, 25^13, 26^5, 27^8, 28^6, 29^8, 30^4, 31^2, 32^9, 33^5, 34^3, 35^2, 37^3, 38, 39, 40^2, 46, 49, 51, 53, 54, 59, 60, 62, 64^2, 71, 83, 91, 101, 103, 108, 586, 4503, 6118]
surviving nonsolo ucounts = 3[586, 4503, 6118]
ids = [1287, 508, 988]

====================================================================================

REJECT CONTIGS

TIG 1[bases=354]
5-242 ==> 5763-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
255-302 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=3)
302-354 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [508, 988, 1287]
of which 3 are surviving nonsolos
reads assigned: 10973
start codons at 119, 129, 203
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1113 = AGCGTATAGTAGTGCG-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1115 = AGCGTATAGTCACGCC-1

using 43 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 12.1121 = AGCGTATAGTGTGAAT-1

using 78 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[3, 6^2, 13, 14, 16, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1123 = AGCGTATAGTGTTGAA-1

using 53 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2, 4^2, 5, 6, 11, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1124 = AGCGTATAGTGTTTGC-1

using 10142 reads

====================================================================================

graph has 5843 edges initially, 124 edges after simplification

total ucounts = 551
nonsolo ucounts = 417[2^58, 3^40, 4^28, 5^29, 6^34, 7^16, 8^33, 9^18, 10^21, 11^15, 12^14, 13^11, 14^7, 15^11, 16^11, 17^8, 18^9, 19^3, 20^8, 21, 22^2, 24^4, 25^2, 26^4, 27^2, 30^2, 31, 34, 35^2, 37, 38, 41, 50, 55, 57, 59, 61, 62, 70^2, 86^2, 103, 109, 278, 421, 502, 526, 697, 1434, 1727]
surviving nonsolo ucounts = 8[50, 278, 421, 502, 526, 697, 1434, 1727]
ids = [353, 503, 7, 527, 411, 358, 121, 548]

====================================================================================

UMI info for barcode AGCGTATAGTGTTTGC-1 contig 1 = GACTGTGATT...
umi TCAAGTACCT = 528 reads: +388 validated
umi TCGGTAGGTC = 9 reads: -379X +1 -1XX +2 -5XX invalidated

UMI info for barcode AGCGTATAGTGTTTGC-1 contig 2 = ACTCCCACCT...
umi AACTGACTGT = 424 reads: +412 validated
umi ATATCCATCA = 1440 reads: +412 validated
umi GTCGCCAGAT = 10 reads: -412X invalidated
umi GTCTTATGCA = 618 reads: +387 -5 +20 non-validated
umi TTCATAACAT = 282 reads: +412 validated
umi TTTATAACTG = 502 reads: +412 validated
umi TTTTTGGTGT = 1741 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=486]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=2)
388-444 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=4)
444-486 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CALSVGNQFYF at 380, score = 9 + 7
umis assigned: [411, 432]
of which 1 are surviving nonsolos
reads assigned: 530
start codons at 15, 56, 131, 219, 273
confident = true

TIG 2[bases=541]
0-77 ==> 0-77 on |578|TRBV15|5'UTR| [len=77] (mis=0)
77-421 ==> 0-344 on |579|TRBV15|L-REGION+V-REGION| [len=344] (mis=0)
440-489 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=4)
489-541 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 5 umis using 197 reads
cdr3 = CATSREMGSPGVFADTQYF at 404, score = 6 + 8
umis assigned: [7, 121, 353, 358, 503, 527, 548]
of which 7 are surviving nonsolos
reads assigned: 4927
start codons at 43, 58, 77, 101, 129, 135, 140, 227, 282, 300, 395, 422
confident = true
now this is a cell
paired!

GGCTCAGTTCAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTGAGTGTCGGTAACCAGTTCTATTTTGGGACAGGGACAAGTTTGACGGTCATTCCAA <==> GCAGCCATGTACCTGTGTGCCACCAGCAGAGAGATGGGTAGCCCCGGCGTATTTGCAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

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BARCODE 12.1129 = AGCGTATCAAATTGCC-1

using 177 reads

====================================================================================

graph has 193 edges initially, 14 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^2, 4, 5, 6, 11, 16, 18^2, 23, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1130 = AGCGTATCAAGAAGAG-1

using 25260 reads

====================================================================================

graph has 12056 edges initially, 312 edges after simplification

total ucounts = 1396
nonsolo ucounts = 1042[2^114, 3^112, 4^80, 5^72, 6^49, 7^54, 8^51, 9^38, 10^27, 11^13, 12^24, 13^19, 14^20, 15^19, 16^20, 17^25, 18^19, 19^18, 20^15, 21^18, 22^22, 23^13, 24^10, 25^9, 26^10, 27^8, 28^7, 29^8, 30^6, 31^5, 32, 33^5, 34^3, 35^2, 36^4, 37^4, 38^4, 39^3, 41^4, 42^5, 44^4, 45^5, 46^2, 47^4, 48^3, 49^3, 50^3, 51^4, 52^3, 53^2, 54^4, 55, 57, 59^2, 60^4, 61^3, 62, 63^3, 64^4, 65^3, 66^4, 67, 68, 69^2, 70, 71, 72^2, 74^2, 75, 78^4, 80^3, 81, 83^2, 86, 88, 90^2, 92, 93, 94, 95, 96, 98, 103, 109, 111^3, 131, 190, 8038]
surviving nonsolo ucounts = 2[190, 8038]
ids = [1004, 221]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1132 = AGCGTATCAATAGAGT-1

using 152 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 3, 5^2, 8, 12, 17, 98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1134 = AGCGTATCAATGCCAT-1

using 176 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^3, 3, 4^2, 8, 52, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1135 = AGCGTATCAATGTTGC-1

using 32 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.17
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk12-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk12-ucc6a7a72e6/files/contig_bam.bam

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109.867 seconds used processing barcodes, peak mem = 0.31
