[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.04 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk06-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk06-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk06.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.0 = ACCGTAACAGTCGTGC-1

using 12668 reads

====================================================================================

graph has 6574 edges initially, 210 edges after simplification

total ucounts = 589
nonsolo ucounts = 405[2^61, 3^40, 4^36, 5^21, 6^24, 7^19, 8^15, 9^15, 10^16, 11^14, 12^10, 13^11, 14^12, 15^14, 16^8, 17^5, 18^2, 19^5, 20^5, 21^2, 22^2, 24^2, 25, 26^3, 27^5, 28^2, 29^3, 30^2, 31^3, 32^2, 33^5, 39, 42^2, 44^2, 45, 46, 47^2, 48^2, 51, 52, 54^4, 56, 58, 66, 67^2, 68, 70, 76, 77, 80, 84, 93, 94^2, 106, 108, 233, 274, 707, 1067, 1208, 1421, 2180]
surviving nonsolo ucounts = 8[84, 233, 274, 707, 1067, 1208, 1421, 2180]
ids = [585, 278, 554, 204, 27, 87, 417, 447]

====================================================================================

UMI info for barcode ACCGTAACAGTCGTGC-1 contig 1 = GACTCTGCTC...
umi CGACAAGCAG = 693 reads: +49 -1XX +2 -2X +322 -16 +8 invalidated
umi GAGGTTGGGT = 234 reads: +365 -28 +7 non-validated
umi TACCCGGGTC = 1200 reads: -13 +1 -1XX +10 -1XX +4 -1XX +1 -1XX +3 -1XX +1 -1XX +5 -1XX +3 -1XX +351 invalidated
umi TTCGTCAATA = 278 reads: +362 -1 +22 -11 +4 non-validated

GOOD CONTIGS

TIG 1[bases=648]
0-196 ==> 12-208 on |589|TRBV19|5'UTR| [len=208] (mis=0)
196-530 ==> 0-334 on |590|TRBV19|L-REGION+V-REGION| [len=344] (mis=0)
534-546 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
547-596 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
596-648 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 72 reads
cdr3 = CATKGQDASTDTQYF at 523, score = 6 + 8
umis assigned: [204, 278, 417, 554]
of which 4 are surviving nonsolos
reads assigned: 2363
start codons at 196, 254, 305, 341, 346, 410, 542
confident = true

REJECT CONTIGS

TIG 1[bases=500]
0-47 ==> 0-47 on |692|TRBV9|5'UTR| [len=47] (mis=0)
47-390 ==> 0-343 on |693|TRBV9|L-REGION+V-REGION| [len=343] (mis=3)
397-448 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
448-500 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CISVPAAPGTVRSGELFF at 366, score = 3 + 9
umis assigned: [27, 87, 447, 585]
of which 4 are surviving nonsolos
reads assigned: 4417
start codons at 3, 47, 169, 255
confident = false
not full
frameshifted full length stopped transcript of length 500
VJ delta = 27
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.3 = ACCGTAACATAACCTG-1

using 98 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.5 = ACCGTAACATATACCG-1

using 61 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^3, 3, 5^2, 6, 9, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.7 = ACCGTAACATCACCCT-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.12 = ACCGTAACATTCCTGC-1

using 148 reads

====================================================================================

graph has 156 edges initially, 8 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^2, 3^3, 5, 6, 9, 10^3, 16, 23, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.15 = ACCGTAAGTAGAAGGA-1

using 38 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.20 = ACCGTAAGTATCGCAT-1

using 52 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.22 = ACCGTAAGTCAGGACA-1

using 88 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.24 = ACCGTAAGTCCAGTTA-1

using 93 reads

====================================================================================

graph has 94 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 4^2, 6, 7, 20^2, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.26 = ACCGTAAGTCCTGCTT-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.27 = ACCGTAAGTCGCGGTT-1

using 84 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.33 = ACCGTAAGTGACAAAT-1

using 76 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.38 = ACCGTAAGTGGGTATG-1

using 2126 reads

====================================================================================

graph has 552 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[6, 9, 2110]
surviving nonsolo ucounts = 1[2110]
ids = [2]

====================================================================================

UMI info for barcode ACCGTAAGTGGGTATG-1 contig 1 = TGGGGAGAAG...
umi CATGTGGTCA = 2207 reads: +129 -3XX +268 invalidated

GOOD CONTIGS

TIG 1[bases=488]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=4)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=5)
391-446 ==> 0-55 on |406|TRAJ21|J-REGION| [len=55] (mis=1)
446-488 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 124 reads
cdr3 = CAYTDNFNKFYF at 379, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 2076
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370, 434
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.43 = ACCGTAAGTTCCACAA-1

using 136 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[3, 5, 13, 14, 18, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.47 = ACCGTAAGTTGAACTC-1

using 118 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.50 = ACCGTAATCAACACAC-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.51 = ACCGTAATCAACACTG-1

using 94 reads

====================================================================================

graph has 22 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[45, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.52 = ACCGTAATCAACGGCC-1

using 73 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[73]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.54 = ACCGTAATCAATAAGG-1

using 34 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 5, 6, 7, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.55 = ACCGTAATCAATCACG-1

using 36048 reads

====================================================================================

graph has 22495 edges initially, 874 edges after simplification

total ucounts = 2179
nonsolo ucounts = 1671[2^230, 3^158, 4^113, 5^111, 6^95, 7^77, 8^67, 9^68, 10^55, 11^54, 12^44, 13^38, 14^36, 15^41, 16^43, 17^31, 18^35, 19^21, 20^22, 21^29, 22^27, 23^28, 24^28, 25^18, 26^19, 27^21, 28^18, 29^17, 30^20, 31^7, 32^12, 33^3, 34^6, 35^3, 36^11, 37^6, 38^6, 40^4, 41^2, 42^2, 43, 44^2, 45^2, 46, 47^3, 48, 49, 50^3, 51^4, 52, 53^2, 58, 61, 64, 72, 73, 75, 85, 88, 90, 149, 220, 229, 445^2, 547, 757, 949, 1110, 1248, 1321, 1740, 1813, 1972, 2936]
surviving nonsolo ucounts = 15[31, 220, 229, 445^2, 547, 757, 949, 1110, 1248, 1321, 1740, 1813, 1972, 2936]
ids = [1930, 1341, 1737, 1159, 1796, 609, 1822, 1547, 2003, 2075, ...]

====================================================================================

UMI info for barcode ACCGTAATCAATCACG-1 contig 1 = TTTTGAAACC...
umi GGGGCACCCC = 208 reads: +364 -7 +29 non-validated
umi TCCCGAAGAC = 201 reads: +342 -58 non-validated
umi TCTCACGCCG = 722 reads: +400 validated

UMI info for barcode ACCGTAATCAATCACG-1 contig 2 = GAGAGTCCTG...
umi AACCCTGGCA = 1795 reads: +29 -1XX +367 invalidated
umi CAGATGAGTA = 487 reads: -378X +2 -2XX +1 -1XX +3 -1XX +1 -2XX +2 -2XX +2 invalidated
umi CATTCCAGAA = 2710 reads: -360 +5 -1XX +2 -1XX +2 -1XX +2 -1XX +6 -1XX +4 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CCGGTGACCG = 1 reads: -393 +2 -1X +1 invalidated
umi GAAACACCTC = 1993 reads: +397 validated
umi GATCGCCGCG = 379 reads: -378X +2 -2XX +1 -1XX +3 -1XX +1 -2XX +2 -2XX +2 invalidated
umi GTTAATCTTT = 1317 reads: +397 validated
umi TAATGTCGCC = 958 reads: +397 validated
umi TGGGCGCTCA = 32 reads: +340 -1X +10 -41 +5 invalidated
umi TTATAGCGCT = 1115 reads: +397 validated
umi TTATCTAATA = 1821 reads: +397 validated
umi TTCTGCCTCT = 1240 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=570]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=3)
471-528 ==> 0-57 on |419|TRAJ33|J-REGION| [len=57] (mis=3)
528-570 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 0 umis using 0 reads
cdr3 = CAVRPRYSNYQLIW at 455, score = 7 + 9
umis assigned: [1341, 1737, 1822]
of which 3 are surviving nonsolos
reads assigned: 1104
start codons at 128, 291, 431
confident = true

TIG 2[bases=515]
0-66 ==> 0-66 on |657|TRBV6-5|5'UTR| [len=66] (mis=0)
66-410 ==> 0-344 on |658|TRBV6-5|L-REGION+V-REGION| [len=344] (mis=5)
416-463 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
463-515 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 557 reads
cdr3 = CASSPRDASYEQYF at 393, score = 9 + 8
umis assigned: [41, 609, 671, 756, 1089, 1159, 1474, 1547, 1930, 2003] and 2 others
of which 11 are surviving nonsolos
reads assigned: 13570
start codons at 24, 52, 66, 124, 177, 201, 208, 216, 243, 307, 316
confident = true

REJECT CONTIGS

TIG 1[bases=499]
3-59 ==> 4502-4558 on segment before TRAV30 exon 1 [len=4558] (mis=0)
59-396 ==> 0-337 on |498|TRAV30|L-REGION+V-REGION| [len=337] (mis=2)
398-457 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=1)
457-499 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [1796]
of which 1 are surviving nonsolos
reads assigned: 443
start codons at 59, 171, 234, 257, 291, 315, 406, 430
confident = false
frameshifted full length stopped transcript of length 499
did not find CDR3
now this is a cell
paired!

CATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGAGACCCCGTTATAGCAACTATCAGTTAATCTGGGGCGCTGGGACCAAGCTAATTATAAAGCCAG <==> GCTGCTCCCTCCCAGACATCTGTGTACTTCTGTGCCAGCAGCCCCCGGGACGCATCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.57 = ACCGTAATCACATAGC-1

using 13548 reads

====================================================================================

graph has 10638 edges initially, 398 edges after simplification

total ucounts = 1169
nonsolo ucounts = 879[2^127, 3^70, 4^67, 5^47, 6^54, 7^37, 8^37, 9^23, 10^31, 11^22, 12^26, 13^22, 14^19, 15^33, 16^27, 17^18, 18^18, 19^21, 20^12, 21^17, 22^12, 23^16, 24^12, 25^12, 26^10, 27^7, 28^13, 29^4, 30^5, 31^7, 32^4, 33^2, 34^6, 35^3, 36^6, 37^5, 38^2, 39^2, 40, 41, 42, 43, 44^2, 45, 47^2, 48, 53, 58, 62, 65, 68, 70, 71, 78, 80, 82, 100, 122, 2312]
surviving nonsolo ucounts = 2[2, 2312]
ids = [680, 401]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.58 = ACCGTAATCACCCTCA-1

using 70 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.60 = ACCGTAATCACGATGT-1

using 33 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[11, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.61 = ACCGTAATCAGCAACT-1

using 13985 reads

====================================================================================

graph has 5917 edges initially, 114 edges after simplification

total ucounts = 715
nonsolo ucounts = 496[2^74, 3^44, 4^38, 5^28, 6^29, 7^32, 8^21, 9^31, 10^17, 11^25, 12^13, 13^20, 14^17, 15^10, 16^16, 17^7, 18, 19^8, 20^5, 21^5, 22^8, 23^3, 24^3, 25, 26^4, 27^4, 28, 29^6, 30^4, 31, 32, 34, 36, 37^2, 38, 39^2, 46, 50, 54, 57, 66, 67, 81, 82, 87, 99, 114, 8348]
surviving nonsolo ucounts = 1[8348]
ids = [518]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.63 = ACCGTAATCAGTACGT-1

using 84 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.70 = ACCGTAATCCAGGGCT-1

using 65 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.71 = ACCGTAATCCATGAGT-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.72 = ACCGTAATCCATGCTC-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.77 = ACCGTAATCCTCATTA-1

using 32 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.78 = ACCGTAATCCTCTAGC-1

using 105 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.83 = ACCGTAATCGCCATAA-1

using 11 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.87 = ACCGTAATCGTTTATC-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.95 = ACCGTAATCTCAAACG-1

using 97 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.96 = ACCGTAATCTCCAACC-1

using 45 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.103 = ACCGTAATCTTACCTA-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.108 = ACCTTTAAGAAACCTA-1

using 98 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2, 4^3, 5, 6^2, 7, 8^2, 9, 11^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.111 = ACCTTTAAGAAGGACA-1

using 101 reads

====================================================================================

graph has 62 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3, 6, 7^2, 13^3, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.112 = ACCTTTAAGAAGGGTA-1

using 73 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[4^3, 5^2, 9, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.113 = ACCTTTAAGAATGTGT-1

using 25 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.116 = ACCTTTAAGACAGACC-1

using 82 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3^2, 6, 8^2, 9, 14^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.119 = ACCTTTAAGACCGGAT-1

using 131 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^3, 3^3, 4, 5, 6, 7, 9, 12, 32, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.122 = ACCTTTAAGACTACAA-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.124 = ACCTTTAAGACTGGGT-1

using 186 reads

====================================================================================

graph has 124 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 15[2, 3, 4^2, 6, 7, 8^2, 9^2, 10, 11, 25, 34, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.126 = ACCTTTAAGAGACTTA-1

using 36 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.127 = ACCTTTAAGAGAGCTC-1

using 117 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2^2, 3, 4, 5^2, 6, 7^2, 13, 14, 22, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.128 = ACCTTTAAGAGCCTAG-1

using 69008 reads

====================================================================================

graph has 25782 edges initially, 645 edges after simplification

total ucounts = 1757
nonsolo ucounts = 1453[2^120, 3^101, 4^62, 5^58, 6^48, 7^41, 8^34, 9^39, 10^31, 11^32, 12^22, 13^25, 14^27, 15^27, 16^27, 17^23, 18^27, 19^25, 20^20, 21^13, 22^17, 23^20, 24^22, 25^15, 26^22, 27^12, 28^13, 29^8, 30^13, 31^20, 32^11, 33^10, 34^13, 35^14, 36^13, 37^12, 38^5, 39^23, 40^9, 41^9, 42^9, 43^9, 44^11, 45^19, 46^6, 47^7, 48^9, 49^11, 50^10, 51^6, 52^5, 53^4, 54^17, 55^8, 56^5, 57^4, 58^6, 59^3, 60^3, 61^11, 62^5, 63^8, 64^9, 65^4, 66^4, 67^7, 68^6, 69^5, 70^8, 71^8, 72^5, 73^7, 74^5, 75^5, 76^9, 77^5, 78^5, 79^7, 80^4, 81^4, 82^5, 83^6, 84^4, 85, 86^2, 87^2, 88^2, 89^4, 90^3, 91^3, 92^4, 93^2, 94^2, 95^3, 97^4, 99, 100^2, 101, 102, 103^2, 104, 106, 108, 109, 110, 111^2, 113, 114, 116, 117^2, 119, 124, 127, 131, 152, 375, 680, 827, 831, 1279, 1391, 1402, 1462, 1735, 1896, 1977, 1987, 2000, 2101, 4448, 5364]
surviving nonsolo ucounts = 15[375, 680, 827, 831, 1279, 1391, 1402, 1462, 1735, 1896, 1987, 2000, 2101, 4448, 5364]
ids = [588, 50, 389, 590, 566, 924, 610, 129, 479, 962, ...]

====================================================================================

UMI info for barcode ACCTTTAAGAGCCTAG-1 contig 1 = GCTCTTTCCC...
umi AAGTGCGAAA = 678 reads: +403 validated
umi ATATACTTTG = 4467 reads: +288 -2XX +2 -1XX +1 -8XX +1 -7XX +2 -1XX +2 -74X +3 -4XX +1 -6XX invalidated
umi CACCTTCTGC = 1747 reads: +403 validated
umi CCACAACGCT = 1277 reads: +403 validated
umi CCATACCTGG = 844 reads: +403 validated
umi CCCCAACAGG = 1411 reads: +403 validated
umi CCCTCGCTTC = 2107 reads: +403 validated
umi CTTCGTGCAC = 1391 reads: +403 validated
umi GACACCGTGT = 1905 reads: +403 validated

UMI info for barcode ACCTTTAAGAGCCTAG-1 contig 2 = TGGGGAAACT...
umi ACCTCGCGCA = 1481 reads: +400 validated
umi GTCATATGGG = 2001 reads: +355 -1XX +2 -2XX +1 -1XX +2 -5XX +1 -5XX +1 -3XX +1 -7XX +2 -1XX +1 -2XX +2 -5XX invalidated

GOOD CONTIGS

TIG 1[bases=516]
0-61 ==> 248-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
61-404 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=2)
399-415 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=3)
413-464 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=1)
464-516 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 8 umis using 651 reads
cdr3 = CASSLLAGGNTGELFF at 388, score = 9 + 9
umis assigned: [50, 319, 479, 566, 590, 610, 629, 924, 962]
of which 9 are surviving nonsolos
reads assigned: 15560
start codons at 61, 343, 347
confident = true

TIG 2[bases=528]
86-417 ==> 0-331 on |508|TRAV39|L-REGION+V-REGION| [len=331] (mis=1)
424-486 ==> 0-62 on |424|TRAJ38|J-REGION| [len=62] (mis=0)
486-528 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CAVDSAFNAGNNRKLIW at 404, score = 6 + 8
umis assigned: [129, 1174]
of which 2 are surviving nonsolos
reads assigned: 3406
start codons at 86, 104, 176, 294, 323, 381, 426
confident = true

REJECT CONTIGS

TIG 1[bases=339]
4-112 ==> 2466-2574 on segment before TRBJ2-1 exon 1 [len=3226] (mis=0)
162-234 ==> 0-72 on segment before TRBJ2-6 exon 1 [len=72] (mis=0)
234-287 ==> 0-53 on |549|TRBJ2-6|J-REGION| [len=53] (mis=0)
287-339 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [588, 1432]
of which 2 are surviving nonsolos
reads assigned: 2294
start codons at 88
confident = false
did not find CDR3

TIG 2[bases=473]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=1)
365-431 ==> 0-66 on |440|TRAJ53|J-REGION| [len=66] (mis=5)
431-473 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CSKYAGASGGSNYKLTF at 349, score = 3 + 9
umis assigned: [389]
of which 1 are surviving nonsolos
reads assigned: 794
start codons at 23, 78, 87, 237, 359
confident = false
frameshifted full length stopped transcript of length 473
VJ delta = 26
not full
not full

TIG 3[bases=347]
5-246 ==> 1235-1476 on segment before TRAJ35 exon 1 [len=1476] (mis=0)
246-305 ==> 0-59 on |421|TRAJ35|J-REGION| [len=59] (mis=1)
305-347 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [262, 1062, 1507, 1612]
of which 1 are surviving nonsolos
reads assigned: 5333
start codons at 46, 70, 119, 128, 133, 195, 203, 211, 233, 260
confident = false
did not find CDR3
now this is a cell
paired!

CTCTCTGCCACCTACTTCTGTGCCGTGGACTCCGCTTTTAATGCTGGCAACAACCGTAAGCTGATTTGGGGATTGGGAACAAGCCTGGCAGTAAATCCGA <==> CTGGGGGACTCGGCCCTTTATCTTTGCGCCAGCAGCTTACTAGCGGGAGGGAACACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.129 = ACCTTTAAGAGCTATA-1

using 70 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.130 = ACCTTTAAGAGCTTCT-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.132 = ACCTTTAAGAGGGATA-1

using 126 reads

====================================================================================

graph has 93 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 3, 4, 7, 10, 20, 21, 24, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.133 = ACCTTTAAGAGGTTAT-1

using 31 reads

====================================================================================

graph has 36 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 4, 10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.134 = ACCTTTAAGAGGTTGC-1

using 200 reads

====================================================================================

graph has 103 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[3^2, 4, 5^2, 7, 12, 14, 18, 21, 22, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.135 = ACCTTTAAGAGTAATC-1

using 36 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.136 = ACCTTTAAGAGTACAT-1

using 20381 reads

====================================================================================

graph has 9832 edges initially, 368 edges after simplification

total ucounts = 1104
nonsolo ucounts = 873[2^89, 3^66, 4^57, 5^55, 6^45, 7^47, 8^46, 9^37, 10^31, 11^29, 12^30, 13^22, 14^26, 15^21, 16^21, 17^9, 18^12, 19^12, 20^22, 21^8, 22^7, 23^15, 24^4, 25^8, 26^9, 27^8, 28^5, 29^13, 30^11, 31^2, 32^7, 33^7, 34^5, 35^3, 36^6, 37^2, 38^3, 39, 40^4, 41^4, 42^6, 43^4, 44^3, 45^4, 46, 47, 48^4, 49^4, 50^2, 51^2, 52^2, 53, 55, 57, 59, 60^3, 61, 62, 63, 66, 67, 70^2, 71, 72, 76, 80, 84, 86, 89, 95, 117, 121, 187, 806, 1007, 1104, 1294, 1485, 1665]
surviving nonsolo ucounts = 7[187, 806, 1007, 1104, 1294, 1485, 1665]
ids = [354, 782, 1033, 17, 971, 221, 655]

====================================================================================

UMI info for barcode ACCTTTAAGAGTACAT-1 contig 1 = GTTCCCCTAT...
umi AAAGATTCGA = 1104 reads: +418 validated
umi ACACTCGCCT = 1 reads: -418 non-validated
umi CATGATCCGA = 186 reads: +401 -8 +9 non-validated
umi GCCACGGCTA = 1664 reads: +418 validated
umi TGCCTAAAGC = 1283 reads: +418 validated

UMI info for barcode ACCTTTAAGAGTACAT-1 contig 2 = GGGGAACTTT...
umi AGTTCTCTAT = 1492 reads: +394 validated
umi GTTTCTTATA = 392 reads: +41 -2XX +300 -34 +17 invalidated
umi TATTGTCAGG = 8 reads: -381X +1 -4XX +1 -6XX +1 invalidated
umi TTCCGAAGAC = 1012 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=527]
0-57 ==> 0-57 on |647|TRBV6-1|5'UTR| [len=57] (mis=0)
57-401 ==> 0-344 on |648|TRBV6-1|L-REGION+V-REGION| [len=344] (mis=4)
402-418 ==> 0-16 on |730|TRBD2|D-REGION| [len=16] (mis=1)
426-475 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
475-527 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 189 reads
cdr3 = CASSPTGTSGRKDGSTDTQYF at 384, score = 9 + 8
umis assigned: [17, 105, 354, 655, 971]
of which 4 are surviving nonsolos
reads assigned: 4168
start codons at 15, 57, 115, 168, 192, 207, 234, 298, 307, 421
confident = true

TIG 2[bases=493]
57-388 ==> 0-331 on |508|TRAV39|L-REGION+V-REGION| [len=331] (mis=4)
394-451 ==> 9-66 on |440|TRAJ53|J-REGION| [len=66] (mis=0)
451-493 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 136 reads
cdr3 = CAGPLLRGSNYKLTF at 375, score = 6 + 9
umis assigned: [221, 782, 855, 1033]
of which 3 are surviving nonsolos
reads assigned: 2852
start codons at 57, 75, 147, 265, 294, 352
confident = true
now this is a cell
paired!

CATGACCTCTCTGCCACCTACTTCTGTGCCGGGCCCCTCCTCCGAGGTAGCAACTATAAACTGACATTTGGAAAAGGAACTCTCTTAACCGTGAATCCAA <==> GTGTACTTCTGTGCCAGCAGTCCTACCGGGACTAGCGGGAGGAAGGATGGATCCACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.137 = ACCTTTAAGATAGTCA-1

using 465 reads

====================================================================================

graph has 403 edges initially, 10 edges after simplification

total ucounts = 19
nonsolo ucounts = 19[2, 3, 5^2, 6, 7, 10^3, 12^2, 15, 16, 21, 27, 28, 58, 97, 121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.138 = ACCTTTAAGATCACGG-1

using 80 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 5^2, 6, 9, 12, 14, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.141 = ACCTTTAAGATCTGCT-1

using 61 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.142 = ACCTTTAAGATGCCAG-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.143 = ACCTTTAAGATGTGTA-1

using 98 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[3, 7, 8^2, 19, 24, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.150 = ACCTTTAAGCGAAGGG-1

using 468 reads

====================================================================================

graph has 338 edges initially, 14 edges after simplification

total ucounts = 27
nonsolo ucounts = 23[2^8, 3, 4, 5^2, 9, 15, 16, 20, 21, 27, 33, 59, 62, 67, 102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.151 = ACCTTTAAGCGAGAAA-1

using 47 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 4, 14, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.156 = ACCTTTAAGCTAGGCA-1

using 140 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 4, 5, 9, 10, 20, 22, 27, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.159 = ACCTTTAAGCTGCAAG-1

using 13043 reads

====================================================================================

graph has 5692 edges initially, 142 edges after simplification

total ucounts = 469
nonsolo ucounts = 347[2^44, 3^36, 4^27, 5^22, 6^17, 7^19, 8^22, 9^14, 10^12, 11^6, 12^12, 13^8, 14^8, 15^5, 16^5, 17^4, 18^7, 19^5, 20^5, 21^3, 22^2, 23^6, 24^5, 25^4, 26^4, 27, 28^2, 32, 33, 37, 38, 39, 40, 41, 42^3, 43^2, 44^4, 49, 50^2, 51, 52, 53, 56, 57, 59, 60, 63^2, 65, 66, 68, 69, 71, 88, 92, 122, 992, 1088, 1119, 1350, 1491, 2236]
surviving nonsolo ucounts = 7[49, 992, 1088, 1119, 1350, 1491, 2236]
ids = [240, 39, 446, 71, 211, 433, 351]

====================================================================================

UMI info for barcode ACCTTTAAGCTGCAAG-1 contig 1 = GGGAGATTCT...
umi ACGTCTACCG = 995 reads: +397 validated
umi GACATTTATA = 1358 reads: +397 validated
umi TTAGATCGCC = 1463 reads: -296X +101 invalidated
umi TTCGCTAATA = 1091 reads: +397 validated

UMI info for barcode ACCTTTAAGCTGCAAG-1 contig 2 = ATGTGGGGCT...
umi TATGGGACAT = 2225 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=551]
112-449 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=0)
443-509 ==> 0-66 on |418|TRAJ32|J-REGION| [len=66] (mis=4)
509-551 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 171 reads
cdr3 = CVVNKDGATNKLIF at 436, score = 7 + 8
umis assigned: [39, 211, 433, 446]
of which 4 are surviving nonsolos
reads assigned: 4816
start codons at 14, 51, 109, 112, 209, 316, 338, 344, 452
confident = true

TIG 2[bases=448]
21-365 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=5)
358-370 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=3)
375-418 ==> 8-51 on |539|TRBJ1-4|J-REGION| [len=51] (mis=0)
418-448 ==> 0-30 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 183 reads
cdr3 = CASSHRGGDEKLFF at 348, score = 6 + 8
umis assigned: [351]
of which 1 are surviving nonsolos
reads assigned: 2203
start codons at 0, 21, 79, 143, 156, 163, 171, 226, 229, 237, 339
confident = true
now this is a cell
paired!

AAGCTCAGTGATTCAGCCACCTACCTCTGTGTGGTGAACAAAGATGGTGCTACAAACAAGCTCATCTTTGGAACTGGCACTCTGCTTGCTGTCCAGCCAA <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGCCACAGGGGGGGGGACGAAAAACTGTTTTTTGGCAGTGGAACCCAGCTCTCTGTCTTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.160 = ACCTTTAAGGATGCGT-1

using 67 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 4, 5^2, 8, 11, 13, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.161 = ACCTTTAAGGCAGTCA-1

using 118 reads

====================================================================================

graph has 64 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 13[2^4, 3, 4^2, 7, 11, 13, 14, 17, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.163 = ACCTTTAAGGCCCTTG-1

using 130 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[130]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.164 = ACCTTTAAGGCGTACA-1

using 182 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 15[3^2, 4^2, 5^3, 6, 9^2, 13, 16, 20, 21, 57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.167 = ACCTTTAAGGGTCGAT-1

using 390 reads

====================================================================================

graph has 308 edges initially, 10 edges after simplification

total ucounts = 39
nonsolo ucounts = 30[2^3, 3^3, 4^4, 5^4, 6^3, 7^3, 8, 9, 10, 11, 15, 17, 31, 55, 60, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.168 = ACCTTTAAGTACACCT-1

using 125 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2, 3, 4^2, 5^2, 6^2, 17, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.169 = ACCTTTAAGTACGCCC-1

using 150 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 13[2^3, 3, 4, 5, 6, 7, 11, 14, 18, 23, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.170 = ACCTTTAAGTAGCGGT-1

using 27 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 8, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.171 = ACCTTTAAGTAGGCCA-1

using 49 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 4^2, 11^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.172 = ACCTTTAAGTCACGCC-1

using 75 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.174 = ACCTTTAAGTCTCGGC-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[23, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.175 = ACCTTTAAGTGAAGTT-1

using 51 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.178 = ACCTTTAAGTGGACGT-1

using 80 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3, 4, 6, 8, 9, 45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.181 = ACCTTTAAGTGTTAGA-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.184 = ACCTTTAAGTTCCACA-1

using 30690 reads

====================================================================================

graph has 11114 edges initially, 270 edges after simplification

total ucounts = 994
nonsolo ucounts = 765[2^102, 3^74, 4^60, 5^58, 6^46, 7^43, 8^37, 9^26, 10^25, 11^22, 12^21, 13^22, 14^14, 15^14, 16^23, 17^5, 18^9, 19^9, 20^14, 21^10, 22^10, 23^9, 24^11, 25^6, 26^4, 27^4, 28^2, 29^6, 30^2, 31^4, 32^5, 33^6, 34^3, 35^2, 37^7, 38^3, 39^3, 40, 41^3, 42^2, 43, 44^4, 46^3, 47, 49, 50, 52, 56, 57, 59, 60^2, 61, 63, 65^2, 67, 76, 81, 83, 112, 129, 167, 378, 559, 1080, 1085, 1584, 1593, 1683, 1685, 4877, 6612]
surviving nonsolo ucounts = 10[378, 559, 1080, 1085, 1584, 1593, 1683, 1685, 4877, 6612]
ids = [422, 924, 572, 181, 198, 352, 419, 766, 358, 578]

====================================================================================

UMI info for barcode ACCTTTAAGTTCCACA-1 contig 1 = AGATCAGAAG...
umi AGGGTGTCGA = 1083 reads: +412 validated
umi AGTTCCTTCT = 1587 reads: +412 validated
umi CGACTACCAA = 1692 reads: +412 validated
umi TATCTTGGTC = 1644 reads: +412 validated
umi TTATTGTTAA = 560 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=500]
0-46 ==> 0-46 on |506|TRAV38-2/DV8|5'UTR| [len=46] (mis=0)
46-395 ==> 0-349 on |507|TRAV38-2/DV8|L-REGION+V-REGION| [len=349] (mis=0)
398-458 ==> 0-60 on |414|TRAJ29|J-REGION| [len=60] (mis=4)
458-500 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 378 reads
cdr3 = CAYRSGDFSGNTPLVF at 379, score = 7 + 9
umis assigned: [181, 198, 419, 766, 924]
of which 5 are surviving nonsolos
reads assigned: 6493
start codons at 46, 51, 103, 136, 179, 238, 278, 362, 370
confident = true

REJECT CONTIGS

TIG 1[bases=505]
0-76 ==> 0-76 on |487|TRAV25|5'UTR| [len=76] (mis=0)
76-403 ==> 0-327 on |488|TRAV25|L-REGION+V-REGION| [len=327] (mis=2)
406-463 ==> 0-57 on |405|TRAJ20|J-REGION| [len=57] (mis=2)
463-505 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [358, 572]
of which 2 are surviving nonsolos
reads assigned: 5827
start codons at 58, 76, 94, 105, 109, 128, 142, 164, 377
confident = false
frameshifted full length stopped transcript of length 505
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.188 = ACCTTTACAAACAACA-1

using 61 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.191 = ACCTTTACAAACTGTC-1

using 51 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.192 = ACCTTTACAAAGCAAT-1

using 74 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[2^2, 3^4, 8, 14, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.194 = ACCTTTACAACAACCT-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.195 = ACCTTTACAACACCTA-1

using 198 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[198]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.196 = ACCTTTACAACCGCCA-1

using 92 reads

====================================================================================

graph has 68 edges initially, 12 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[41, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.197 = ACCTTTACAAGTCTAC-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.199 = ACCTTTACAAGTTAAG-1

using 93 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2^2, 3^4, 6, 9, 15, 16, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.201 = ACCTTTACAAGTTGTC-1

using 50 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 7[2, 3^2, 4, 9^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.202 = ACCTTTACAATAACGA-1

using 48 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[14, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.205 = ACCTTTACAATCGAAA-1

using 2339 reads

====================================================================================

graph has 668 edges initially, 4 edges after simplification

total ucounts = 20
nonsolo ucounts = 16[2^2, 3^3, 4^2, 6, 10^2, 12, 20, 21, 28, 31, 2176]
surviving nonsolo ucounts = 1[2176]
ids = [17]

====================================================================================

UMI info for barcode ACCTTTACAATCGAAA-1 contig 1 = TGGGACAGTG...
umi TATCACCACA = 2175 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=494]
51-398 ==> 0-347 on |688|TRBV7-9|L-REGION+V-REGION| [len=347] (mis=1)
402-442 ==> 9-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
442-494 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 139 reads
cdr3 = CASSLDLTQYF at 381, score = 7 + 8
umis assigned: [17]
of which 1 are surviving nonsolos
reads assigned: 2142
start codons at 51, 75, 160, 259, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.206 = ACCTTTACAATCGGTT-1

using 103 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[3^2, 4, 5, 10, 13, 14, 16^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.207 = ACCTTTACAATGGATA-1

using 62 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 9[2^2, 3, 4, 5, 9, 12^2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.209 = ACCTTTACACAGACAG-1

using 17 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.210 = ACCTTTACACAGACTT-1

using 68 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.211 = ACCTTTACACAGGAGT-1

using 130 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[4, 5, 8, 10, 12, 17, 20, 24, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.212 = ACCTTTACACAGGCCT-1

using 33 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.213 = ACCTTTACACAGGTTT-1

using 14 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.214 = ACCTTTACACAGTCGC-1

using 34 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.215 = ACCTTTACACATAACC-1

using 93 reads

====================================================================================

graph has 74 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 8, 14, 15, 16, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.216 = ACCTTTACACATCCAA-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.217 = ACCTTTACACATGACT-1

using 14 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.219 = ACCTTTACACCCTATC-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[5, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.221 = ACCTTTACACGAAGCA-1

using 64 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.224 = ACCTTTACACGTTGGC-1

using 106 reads

====================================================================================

graph has 82 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[2, 3^2, 4, 6, 7, 9, 24, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.226 = ACCTTTACACTTGGAT-1

using 81 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.227 = ACCTTTACAGAAGCAC-1

using 70 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2^4, 5^2, 7, 13, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.229 = ACCTTTACAGACAGGT-1

using 2608 reads

====================================================================================

graph has 804 edges initially, 8 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2^2, 3^2, 9, 13, 14, 16, 34, 1244, 1265]
surviving nonsolo ucounts = 2[1244, 1265]
ids = [4, 7]

====================================================================================

UMI info for barcode ACCTTTACAGACAGGT-1 contig 1 = GTCATCCCTC...
umi CACTCATCGG = 1250 reads: +397 validated
umi CGGCCAGGCT = 1273 reads: -9X +1 -1XX +1 -1XX +1 -1XX +1 -1XX +1 -7XX +372 invalidated

GOOD CONTIGS

TIG 1[bases=527]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=2)
424-475 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=4)
475-527 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 2 umis using 174 reads
cdr3 = CSARSLDRALTGELFF at 399, score = 7 + 9
umis assigned: [4, 7]
of which 2 are surviving nonsolos
reads assigned: 2483
start codons at 21, 78, 216, 252, 258, 271, 331
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.232 = ACCTTTACAGATGGGT-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.233 = ACCTTTACAGCCAATT-1

using 134 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[3^3, 4^2, 6, 7, 8, 15^2, 26, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.234 = ACCTTTACAGCCACCA-1

using 32 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.235 = ACCTTTACAGCCTATA-1

using 74 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.240 = ACCTTTACAGCTGTTA-1

using 10103 reads

====================================================================================

graph has 6904 edges initially, 246 edges after simplification

total ucounts = 763
nonsolo ucounts = 649[2^66, 3^43, 4^32, 5^25, 6^28, 7^42, 8^14, 9^28, 10^28, 11^24, 12^30, 13^27, 14^13, 15^21, 16^24, 17^19, 18^11, 19^12, 20^9, 21^14, 22^9, 23^6, 24^10, 25^9, 26^7, 27^10, 28^6, 29^3, 30^5, 31^2, 32^3, 33^2, 34^6, 35, 36^7, 37^6, 38, 39^2, 40, 41^4, 42^3, 43, 44^2, 45, 46^2, 47^2, 48^2, 49^2, 51^3, 52, 53, 55, 56^3, 57, 58, 59, 60, 62, 64, 67^2, 73, 76, 77, 85, 98, 126, 183]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.241 = ACCTTTACAGCTTCGG-1

using 85 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 7, 11, 15, 20, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.243 = ACCTTTACAGGCGATA-1

using 152 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2^3, 3, 7, 9, 10, 13, 14^2, 16, 17, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.245 = ACCTTTACAGTAAGAT-1

using 69 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.246 = ACCTTTACAGTATAAG-1

using 12 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.248 = ACCTTTACAGTGAGTG-1

using 151 reads

====================================================================================

graph has 76 edges initially, 8 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2^3, 3, 4^2, 14, 30, 38, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.249 = ACCTTTACAGTTAACC-1

using 96 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.251 = ACCTTTACAGTTCCCT-1

using 87 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 84]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.252 = ACCTTTACATACGCCG-1

using 173 reads

====================================================================================

graph has 94 edges initially, 6 edges after simplification

total ucounts = 14
nonsolo ucounts = 12[2, 4, 6, 7, 8, 15, 16^2, 18^2, 26, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.253 = ACCTTTACATATGGTC-1

using 95 reads

====================================================================================

graph has 34 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 7[2, 4, 10, 11, 13^2, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.254 = ACCTTTACATCACAAC-1

using 143 reads

====================================================================================

graph has 94 edges initially, 10 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[17, 27, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.256 = ACCTTTACATCCCATC-1

using 60 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.257 = ACCTTTACATCCGTGG-1

using 76 reads

====================================================================================

graph has 54 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[34, 42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.258 = ACCTTTACATCCTTGC-1

using 6341 reads

====================================================================================

graph has 4085 edges initially, 162 edges after simplification

total ucounts = 677
nonsolo ucounts = 473[2^77, 3^58, 4^33, 5^33, 6^24, 7^25, 8^23, 9^21, 10^14, 11^18, 12^17, 13^11, 14^8, 15^9, 16^3, 17^11, 18^5, 19^5, 20^6, 21^4, 22^6, 23^5, 24^3, 25^2, 26^6, 27^4, 28^5, 30^3, 31, 32, 33^5, 34, 36^3, 37, 38^3, 40^3, 41^2, 42^2, 44, 50^2, 51, 52, 55^2, 60, 109, 110, 141, 817]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.262 = ACCTTTACATCGGGTC-1

using 150 reads

====================================================================================

graph has 125 edges initially, 12 edges after simplification

total ucounts = 24
nonsolo ucounts = 21[2^4, 3^2, 4^5, 5, 7^3, 8^2, 10, 13, 15, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.266 = ACCTTTACATGCAACT-1

using 57 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.267 = ACCTTTACATGCTAGT-1

using 62 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.270 = ACCTTTACATTCTCAT-1

using 258 reads

====================================================================================

graph has 166 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[112, 146]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.271 = ACCTTTACATTCTTAC-1

using 66 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.272 = ACCTTTACATTGAGCT-1

using 105 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.274 = ACCTTTACATTTCAGG-1

using 66 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.275 = ACCTTTAGTAATAGCA-1

using 38 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.276 = ACCTTTAGTACTTCTT-1

using 81 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 9, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.277 = ACCTTTAGTAGAAAGG-1

using 21355 reads

====================================================================================

graph has 13197 edges initially, 642 edges after simplification

total ucounts = 1487
nonsolo ucounts = 1183[2^128, 3^86, 4^76, 5^67, 6^53, 7^47, 8^37, 9^43, 10^36, 11^33, 12^31, 13^26, 14^29, 15^26, 16^32, 17^17, 18^12, 19^18, 20^16, 21^16, 22^15, 23^19, 24^16, 25^17, 26^13, 27^19, 28^19, 29^5, 30^17, 31^12, 32^10, 33^6, 34^5, 35^12, 36^9, 37^9, 38^6, 39^3, 40^4, 41^9, 42^5, 43^5, 44^11, 45^7, 46^2, 47^3, 48^4, 49^5, 50^4, 51^6, 52^5, 53^2, 54^6, 55^6, 56^3, 57^3, 58^3, 59, 60^7, 62, 65, 66^4, 67^2, 69^3, 71^2, 72^2, 73, 74^3, 75^2, 76^2, 77, 78^2, 79, 81^2, 82, 87, 88, 89, 91, 98, 102, 106, 109, 111, 112, 123]
surviving nonsolo ucounts = 2[3, 78]
ids = [423, 37]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.278 = ACCTTTAGTAGAAGGA-1

using 33 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.280 = ACCTTTAGTAGAGTGC-1

using 84 reads

====================================================================================

graph has 58 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3, 4, 5, 7, 8, 9, 15^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.281 = ACCTTTAGTAGCGATG-1

using 44 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.282 = ACCTTTAGTAGCTCCG-1

using 85 reads

====================================================================================

graph has 112 edges initially, 8 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4, 7, 9^2, 11, 12, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.283 = ACCTTTAGTAGCTGCC-1

using 69 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 6[2^2, 4, 9, 10, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.284 = ACCTTTAGTAGCTTGT-1

using 83 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.289 = ACCTTTAGTCACTGGC-1

using 79 reads

====================================================================================

graph has 54 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[4, 14, 16, 21, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.291 = ACCTTTAGTCATGCAT-1

using 157 reads

====================================================================================

graph has 134 edges initially, 8 edges after simplification

total ucounts = 12
nonsolo ucounts = 10[4, 6, 8, 9^2, 12, 17, 20, 21, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.295 = ACCTTTAGTCCCTTGT-1

using 82 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 71]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.298 = ACCTTTAGTCCTCCAT-1

using 233 reads

====================================================================================

graph has 176 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^3, 4, 5, 6, 7, 8, 11^2, 13, 17, 22, 23, 26, 32, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.299 = ACCTTTAGTCGAACAG-1

using 40 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.300 = ACCTTTAGTCGCTTTC-1

using 159 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[67, 92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.301 = ACCTTTAGTCGGATCC-1

using 123 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2, 5, 6, 7, 8^3, 10, 11, 24, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.303 = ACCTTTAGTCTCAACA-1

using 35 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.306 = ACCTTTAGTCTCTCGT-1

using 81 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3, 5^2, 6, 13, 14, 16^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.311 = ACCTTTAGTGCATCTA-1

using 250 reads

====================================================================================

graph has 204 edges initially, 8 edges after simplification

total ucounts = 26
nonsolo ucounts = 19[2^3, 3, 4^3, 8, 10, 11^2, 12^2, 16, 17, 18, 21, 39, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.312 = ACCTTTAGTGCTAGCC-1

using 113 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.314 = ACCTTTAGTGGGTCAA-1

using 121 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[121]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.315 = ACCTTTAGTGGTCCGT-1

using 59 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.316 = ACCTTTAGTGTGAATA-1

using 674 reads

====================================================================================

graph has 514 edges initially, 16 edges after simplification

total ucounts = 29
nonsolo ucounts = 25[3^2, 6^3, 10^2, 11^3, 12, 13^2, 15, 17, 20, 22, 27, 44, 46, 50, 67, 75, 84, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.317 = ACCTTTAGTGTGACCC-1

using 72 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.318 = ACCTTTAGTGTGCCTG-1

using 36 reads

====================================================================================

graph has 28 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 6, 8, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.319 = ACCTTTAGTGTTCTTT-1

using 120 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[3, 6, 16, 18, 23, 49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.320 = ACCTTTAGTTAAGATG-1

using 98 reads

====================================================================================

graph has 66 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 3, 5, 7, 8, 10, 12, 20, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.321 = ACCTTTAGTTAAGTAG-1

using 31 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.325 = ACCTTTAGTTCCATGA-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.329 = ACCTTTAGTTGCTCCT-1

using 135 reads

====================================================================================

graph has 132 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[2, 4, 5, 9^4, 26, 59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.333 = ACCTTTAGTTTAAGCC-1

using 62 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.334 = ACCTTTAGTTTGTGTG-1

using 56 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.335 = ACCTTTATCAACACCA-1

using 103 reads

====================================================================================

graph has 202 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2, 3, 6, 7, 8, 11, 22, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.336 = ACCTTTATCAACGAAA-1

using 134 reads

====================================================================================

graph has 110 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 14[2^2, 4^2, 7, 8, 9, 11, 12, 13, 14^2, 15, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.337 = ACCTTTATCAACGCTA-1

using 90 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.340 = ACCTTTATCAAGCCTA-1

using 108 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[3^3, 4^2, 5, 6, 7^2, 18, 22, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.341 = ACCTTTATCAAGGTAA-1

using 80000 reads

====================================================================================

graph has 26263 edges initially, 506 edges after simplification

total ucounts = 2034
nonsolo ucounts = 1586[2^176, 3^111, 4^119, 5^100, 6^88, 7^67, 8^92, 9^65, 10^54, 11^60, 12^52, 13^46, 14^46, 15^33, 16^41, 17^32, 18^24, 19^32, 20^21, 21^21, 22^21, 23^25, 24^12, 25^22, 26^14, 27^12, 28^9, 29^14, 30^12, 31^10, 32^6, 33^4, 34^4, 35^5, 36^8, 37^4, 38^8, 39^3, 40^2, 41^5, 42^3, 43^5, 44^6, 45^3, 46^6, 47^2, 48^3, 49, 51^3, 52^2, 53^2, 54, 55^2, 57^2, 58, 59, 61^2, 62^2, 63, 65, 66^3, 68, 70, 71, 72, 73^2, 74, 77^2, 86, 92, 185, 187, 254, 290, 424, 541, 585, 616, 653, 668, 746, 761, 787, 861, 1044, 1087, 1101, 1187, 1263, 1323, 1453, 1525, 1698, 1701, 1735, 1738, 1756, 1765, 1770, 1794, 1855, 1865, 1914, 2019, 2025, 2026, 2062, 2207, 2259, 2439, 2442, 2530, 2636]
surviving nonsolo ucounts = 40[187, 290, 541, 585, 616, 653, 668, 746, 761, 787, 861, 1044, 1087, 1101, 1187, 1263, 1323, 1453, 1525, 1698, 1701, 1735, 1738, 1756, 1765, 1770, 1794, 1855, 1865, 1914, 2019, 2025, 2026, 2062, 2207, 2259, 2439, 2442, 2530, 2636]
ids = [541, 1503, 189, 392, 900, 353, 1070, 1405, 1823, 858, ...]

====================================================================================

UMI info for barcode ACCTTTATCAAGGTAA-1 contig 1 = ACAGTGATCC...
umi ACCTCTGGAC = 540 reads: +406 validated
umi ACTCCGGAGC = 1518 reads: +406 validated
umi CACCAGGGTT = 1997 reads: -287X +1 -1XX +83 -1XX +33 invalidated
umi CACGTGTATA = 1766 reads: +406 validated
umi CCCTTAATCG = 1925 reads: +406 validated
umi CGCTACTGGC = 612 reads: +406 validated
umi GTGCATCCAC = 2057 reads: +406 validated
umi TAGTATTCAG = 2361 reads: -13 +5 -1XX +4 -6XX +2 -12XX +1 -2XX +1 -2XX +357 invalidated
umi TCCTGTATCT = 1708 reads: +406 validated
umi TGCACCGCCT = 2258 reads: +406 validated
umi TTAAATGCGT = 1012 reads: -308 +98 non-validated

UMI info for barcode ACCTTTATCAAGGTAA-1 contig 2 = GGGACATGAA...
umi CTATTGACGT = 1759 reads: +388 validated
umi CTCAGCTCAA = 850 reads: +388 validated
umi TATTATGGTT = 1797 reads: +388 validated
umi TGGCAGTATG = 1273 reads: +388 validated

UMI info for barcode ACCTTTATCAAGGTAA-1 contig 3 = AGACACCTGC...
umi ATGGGATCAG = 577 reads: +388 validated
umi CATATCATCG = 1763 reads: +240 -1XX +147 invalidated
umi TAAGTTGGTG = 760 reads: -3 +385 non-validated
umi TATCATCCTC = 2447 reads: +388 validated
umi TCCCGCCATC = 2556 reads: +388 validated
umi TTCACCCCAG = 2165 reads: +388 validated

UMI info for barcode ACCTTTATCAAGGTAA-1 contig 4 = CCTTTTCACC...
umi ATCCACATCT = 582 reads: -369 +2 -1X +2 -1X +3 -1X +1 -1X +2 -1XX +8 -2XX +7 -1XX +1 invalidated
umi CATCCAGGAA = 1852 reads: +403 validated
umi CCCTGAAGTC = 1759 reads: +403 validated
umi CCGTCCGAAT = 1169 reads: +403 validated
umi CTATACTGGC = 1739 reads: +403 validated
umi CTTAGCGGCG = 1446 reads: +403 validated
umi GGTAATGCAT = 2000 reads: -24X +1 -3XX +1 -3XX +2 -4XX +1 -1XX +1 -1XX +1 -2XX +358 invalidated
umi TCCGACATCT = 1920 reads: +228 -1XX +1 -1X +172 invalidated
umi TCGATTTCCA = 2020 reads: +403 validated
umi TGTAATACTG = 748 reads: +403 validated

UMI info for barcode ACCTTTATCAAGGTAA-1 contig 5 = GATCTTAATT...
umi CAACGAATCC = 2664 reads: +400 validated
umi CCTTATGAGC = 777 reads: +400 validated
umi GGTTTTCTCA = 1068 reads: +400 validated
umi TACAGGCCCC = 1273 reads: +400 validated
umi TCGTTTATCA = 1614 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=505]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=1)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=5)
413-453 ==> 7-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=0)
453-505 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 909 reads
cdr3 = CASSLGRGQVLREQYF at 377, score = 8 + 8
umis assigned: [189, 224, 484, 502, 761, 900, 1355, 1498, 1661, 1772] and 1 others
of which 11 are surviving nonsolos
reads assigned: 17473
start codons at 47, 156
confident = false

TIG 2[bases=514]
0-84 ==> 0-84 on |453|TRAV1-2|5'UTR| [len=84] (mis=3)
84-402 ==> 0-318 on |454|TRAV1-2|L-REGION+V-REGION| [len=318] (mis=0)
408-472 ==> 0-64 on |395|TRAJ10|J-REGION| [len=64] (mis=4)
472-514 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 4 umis using 269 reads
cdr3 = CAVRDPTGGGNKLTF at 396, score = 7 + 8
umis assigned: [994, 1005, 1554, 1801]
of which 4 are surviving nonsolos
reads assigned: 5594
start codons at 5, 33, 51, 84, 106, 114, 120, 162, 247, 280, 289, 372
confident = false

TIG 3[bases=461]
0-21 ==> 22-43 on |610|TRBV27|5'UTR| [len=43] (mis=0)
21-365 ==> 0-344 on |611|TRBV27|L-REGION+V-REGION| [len=344] (mis=4)
370-409 ==> 11-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=0)
409-461 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 729 reads
cdr3 = CASSPIGAQHF at 348, score = 7 + 8
umis assigned: [392, 572, 1405, 1523, 1640, 1932]
of which 6 are surviving nonsolos
reads assigned: 10050
start codons at 13, 21, 43, 156, 163, 171, 225, 229, 253, 385
confident = false

TIG 4[bases=508]
0-53 ==> 27-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
53-390 ==> 0-337 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=0)
407-456 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=2)
456-508 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 9 umis using 956 reads
cdr3 = CASTPATSGGTDTQYF at 380, score = 9 + 8
umis assigned: [353, 586, 754, 791, 982, 1089, 1279, 1649, 1679, 1823]
of which 10 are surviving nonsolos
reads assigned: 14931
start codons at 11, 53, 111, 164, 188, 195, 203, 230, 294, 303
confident = false

TIG 5[bases=465]
0-23 ==> 0-23 on |463|TRAV13-1|5'UTR| [len=23] (mis=0)
23-360 ==> 0-337 on |464|TRAV13-1|L-REGION+V-REGION| [len=337] (mis=1)
365-423 ==> 8-66 on |432|TRAJ45|J-REGION| [len=66] (mis=0)
423-465 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 5 umis using 443 reads
cdr3 = CAASITGGGADGLTF at 347, score = 9 + 8
umis assigned: [448, 858, 1293, 1428, 1697]
of which 5 are surviving nonsolos
reads assigned: 7263
start codons at 23, 78, 87, 237
confident = false

REJECT CONTIGS

TIG 1[bases=488]
0-66 ==> 0-66 on |451|TRAV1-1|5'UTR| [len=66] (mis=0)
66-392 ==> 0-326 on |452|TRAV1-1|L-REGION+V-REGION| [len=326] (mis=4)
384-446 ==> 0-62 on |446|TRAJ6|J-REGION| [len=62] (mis=3)
446-488 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [541, 671, 1070, 1503]
of which 4 are surviving nonsolos
reads assigned: 2186
start codons at 33, 49, 66, 88, 96, 102, 205, 229, 232, 262, 271, 325, 354
confident = false
frameshifted full length transcript of length 488
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.343 = ACCTTTATCACAGTAC-1

using 42 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.344 = ACCTTTATCACATGCA-1

using 38 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.346 = ACCTTTATCACGCGGT-1

using 59 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.348 = ACCTTTATCACTGGGC-1

using 18 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.353 = ACCTTTATCAGTACGT-1

using 63 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.354 = ACCTTTATCATAACCG-1

using 99 reads

====================================================================================

graph has 64 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[4, 5, 9, 10, 12, 15, 18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.355 = ACCTTTATCATCACCC-1

using 53 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.356 = ACCTTTATCATGGTCA-1

using 155 reads

====================================================================================

graph has 114 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[5, 6, 20, 45, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.358 = ACCTTTATCATGTGGT-1

using 22 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.365 = ACCTTTATCCATTCTA-1

using 77 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^5, 4, 5, 6^2, 7, 8^2, 10^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.366 = ACCTTTATCCCATTTA-1

using 40 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.367 = ACCTTTATCCCTCTTT-1

using 68 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.370 = ACCTTTATCCCTTGTG-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.372 = ACCTTTATCCGCTGTT-1

using 130 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[130]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.373 = ACCTTTATCCTATTCA-1

using 153 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[59, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.375 = ACCTTTATCCTCAATT-1

using 42 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.377 = ACCTTTATCCTTGGTC-1

using 82 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[4, 78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.378 = ACCTTTATCCTTTCTC-1

using 90 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.379 = ACCTTTATCGAATGCT-1

using 36 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.382 = ACCTTTATCGCCAAAT-1

using 1421 reads

====================================================================================

graph has 468 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[1421]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.384 = ACCTTTATCGCGGATC-1

using 4508 reads

====================================================================================

graph has 3329 edges initially, 114 edges after simplification

total ucounts = 386
nonsolo ucounts = 304[2^39, 3^31, 4^21, 5^16, 6^10, 7^17, 8^23, 9^12, 10^8, 11^9, 12^10, 13^6, 14^6, 15^6, 16^2, 17^10, 18^5, 19^7, 20^2, 21^7, 22^6, 23^2, 24, 25^2, 26^3, 27^2, 28^4, 29, 30, 31, 32^2, 33^3, 35^2, 36, 38, 39, 40^3, 41, 44, 46^2, 51^2, 52, 56, 57^2, 60, 61, 68, 73^2, 75, 76, 85, 92, 98, 143]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.385 = ACCTTTATCGCTAGCG-1

using 206 reads

====================================================================================

graph has 169 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[2^2, 3, 9, 13, 17, 21, 23, 25, 26, 28, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.387 = ACCTTTATCGGAAATA-1

using 80 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.389 = ACCTTTATCGGCTTGG-1

using 139 reads

====================================================================================

graph has 89 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 13[2^2, 3^2, 4^2, 8, 9, 16, 20, 21, 22, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.394 = ACCTTTATCTAACGGT-1

using 543 reads

====================================================================================

graph has 530 edges initially, 20 edges after simplification

total ucounts = 36
nonsolo ucounts = 31[2^2, 3^6, 4^2, 6, 8, 9, 11, 13^2, 14^2, 15, 17, 18, 24^2, 26, 29, 31, 35, 38, 44, 58, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.397 = ACCTTTATCTGACCTC-1

using 98 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.398 = ACCTTTATCTGATTCT-1

using 154 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 11[2, 3, 4, 6^2, 8^2, 20, 23, 36, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.401 = ACCTTTATCTGGTTCC-1

using 111 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 16
nonsolo ucounts = 10[2, 3^3, 6, 14, 15, 16, 19, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.403 = ACCTTTATCTTCCTTC-1

using 116 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 110]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.406 = ACCTTTATCTTGGGTA-1

using 100 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2, 3^3, 4, 5, 7^2, 12, 13, 14, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.407 = ACCTTTATCTTGTACT-1

using 48 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.410 = ACCTTTATCTTTCCTC-1

using 58 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.413 = ACGAGCCAGACTAGAT-1

using 81 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.414 = ACGAGCCAGAGCCTAG-1

using 145 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 11, 130]
surviving nonsolo ucounts = 1[130]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.415 = ACGAGCCAGAGTAAGG-1

using 85 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 13, 68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.417 = ACGAGCCAGAGTCTGG-1

using 27 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.418 = ACGAGCCAGATACACA-1

using 51 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 3, 4, 5, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.426 = ACGAGCCAGCGTAATA-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.433 = ACGAGCCAGCTGCGAA-1

using 172 reads

====================================================================================

graph has 92 edges initially, 8 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 7, 13, 24, 27, 29, 63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.435 = ACGAGCCAGGAACTGC-1

using 38 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.437 = ACGAGCCAGGCTATCT-1

using 80 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.440 = ACGAGCCAGGGTGTTG-1

using 66 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.443 = ACGAGCCAGTACGATA-1

using 1042 reads

====================================================================================

graph has 323 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 59, 979]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.445 = ACGAGCCAGTCGTACT-1

using 28 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.450 = ACGAGCCAGTGCCAGA-1

using 55 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.458 = ACGAGCCCAACGATGG-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4^2, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.459 = ACGAGCCCAACTGCTA-1

using 179 reads

====================================================================================

graph has 184 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 24, 30, 119]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.461 = ACGAGCCCAAGGACTG-1

using 33 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.464 = ACGAGCCCAATCTACG-1

using 62 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.465 = ACGAGCCCACACGCTG-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.467 = ACGAGCCCACAGGTTT-1

using 28142 reads

====================================================================================

graph has 8001 edges initially, 64 edges after simplification

total ucounts = 545
nonsolo ucounts = 368[2^75, 3^51, 4^29, 5^27, 6^21, 7^16, 8^20, 9^12, 10^18, 11^13, 12^11, 13^6, 14^7, 15^9, 16^7, 17^4, 18^5, 19^3, 20^2, 21^3, 22^2, 23^3, 24^2, 25^2, 27, 28, 29, 30, 42, 63, 66, 116, 447, 651, 1079, 1382, 1468, 1753, 1885, 1993, 2074, 2440, 3115, 6810]
surviving nonsolo ucounts = 13[116, 447, 651, 1079, 1382, 1468, 1753, 1885, 1993, 2074, 2440, 3115, 6810]
ids = [159, 475, 225, 244, 344, 171, 308, 121, 99, 540, ...]

====================================================================================

UMI info for barcode ACGAGCCCACAGGTTT-1 contig 1 = GGGGAGGTGC...
umi ATACGCGCTA = 2021 reads: +394 validated
umi CAGCGCGTAG = 101 reads: -340 +2 -3X +1 -2X +46 invalidated
umi CATCAGATCT = 1390 reads: +394 validated
umi CGCTGGACTG = 661 reads: +394 validated
umi CTATATTCAA = 657 reads: +371 -1 +22 non-validated
umi GCATAGACAG = 1718 reads: +394 validated
umi TAGCAACAGG = 2460 reads: +394 validated
umi TTTTCTCTCA = 2086 reads: +394 validated

UMI info for barcode ACGAGCCCACAGGTTT-1 contig 2 = GGGGACACTA...
umi GGTTCAGCCT = 1374 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=479]
33-376 ==> 0-343 on |639|TRBV5-5|L-REGION+V-REGION| [len=343] (mis=2)
377-427 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=3)
427-479 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 6 umis using 731 reads
cdr3 = CASSLDTNQPQHF at 360, score = 8 + 8
umis assigned: [99, 159, 171, 225, 244, 308, 391, 540]
of which 8 are surviving nonsolos
reads assigned: 10892
start codons at 33, 155, 238, 319, 403
confident = true

TIG 2[bases=428]
13-345 ==> 0-332 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=1)
347-410 ==> 0-63 on |431|TRAJ44|J-REGION| [len=63] (mis=2)
junction support: 1 umis using 100 reads
cdr3 = CALPITGTASKLTF at 337, score = 9 + 9
umis assigned: [344]
of which 1 are surviving nonsolos
reads assigned: 1360
start codons at 13, 88, 176, 230
confident = true
now this is a cell
paired!

CAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTCCCAATTACCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCG <==> GCCTTGTTGCTGGGGGACTCGGCCCTGTATCTCTGTGCCAGCAGCCTGGACACAAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.470 = ACGAGCCCACCAGATT-1

using 49 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.471 = ACGAGCCCACCAGCAC-1

using 19 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.474 = ACGAGCCCACGACTCG-1

using 103 reads

====================================================================================

graph has 174 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.475 = ACGAGCCCACGGCTAC-1

using 96 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.476 = ACGAGCCCACGGTGTC-1

using 18 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.479 = ACGAGCCCAGACAGGT-1

using 315 reads

====================================================================================

graph has 182 edges initially, 12 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2, 3, 4^3, 5^2, 8^2, 9, 12, 13, 16, 17, 28, 46, 49, 77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.482 = ACGAGCCCAGCGTAAG-1

using 26551 reads

====================================================================================

graph has 12722 edges initially, 340 edges after simplification

total ucounts = 1484
nonsolo ucounts = 1100[2^199, 3^128, 4^121, 5^83, 6^54, 7^46, 8^40, 9^61, 10^39, 11^23, 12^27, 13^27, 14^33, 15^20, 16^25, 17^10, 18^16, 19^14, 20^15, 21^7, 22^7, 23^3, 24^7, 25^5, 26, 27^3, 28^5, 29^2, 30^5, 31^4, 32^2, 33^2, 34, 36^3, 37^3, 38^3, 39^5, 40^4, 41, 42, 43^2, 44^2, 52, 54^3, 55, 56^2, 58^2, 60^3, 61^3, 62^4, 63, 66^2, 71^2, 73, 74, 77, 80, 84, 85, 94, 99^2, 102, 114, 116, 129, 136, 910, 6589, 6810]
surviving nonsolo ucounts = 3[910, 6589, 6810]
ids = [1051, 303, 680]

====================================================================================

REJECT CONTIGS

TIG 1[bases=337]
5-84 ==> 25-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
84-133 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
133-235 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
285-337 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [303, 471, 680, 1051]
of which 3 are surviving nonsolos
reads assigned: 13794
start codons at 
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.483 = ACGAGCCCAGCTGTGC-1

using 37 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^2, 4, 6, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.486 = ACGAGCCCAGGTCTCG-1

using 50 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 3, 12, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.489 = ACGAGCCCATACTCTT-1

using 27 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 5, 8^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.493 = ACGAGCCCATCCTTGC-1

using 97 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.495 = ACGAGCCCATCGTCGG-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.497 = ACGAGCCCATCTCGCT-1

using 22 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.506 = ACGAGCCGTAAGTGTA-1

using 34 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.509 = ACGAGCCGTACCGCTG-1

using 1356 reads

====================================================================================

graph has 558 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 535, 818]
surviving nonsolo ucounts = 2[535, 818]
ids = [0, 3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.510 = ACGAGCCGTAGCGCTC-1

using 60 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.513 = ACGAGCCGTCAAAGCG-1

using 14 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.514 = ACGAGCCGTCAACTGT-1

using 30 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.516 = ACGAGCCGTCATCCCT-1

using 65 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.524 = ACGAGCCGTGCACCAC-1

using 57 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.527 = ACGAGCCGTGTGAATA-1

using 112 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[5, 7, 10, 23, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.528 = ACGAGCCGTTAAGAAC-1

using 70 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.531 = ACGAGCCGTTACGTCA-1

using 8897 reads

====================================================================================

graph has 4353 edges initially, 98 edges after simplification

total ucounts = 515
nonsolo ucounts = 342[2^64, 3^38, 4^31, 5^21, 6^22, 7^26, 8^10, 9^12, 10^14, 11^15, 12^19, 13^5, 14^10, 15^10, 16^6, 17^2, 18^3, 20^3, 21^4, 22^2, 23, 24^3, 25, 26^5, 27, 30, 31, 33, 34, 37, 39, 43, 66, 74, 78, 79, 104, 720, 4846]
surviving nonsolo ucounts = 3[79, 720, 4846]
ids = [404, 314, 499]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.534 = ACGAGCCGTTCCATGA-1

using 4002 reads

====================================================================================

graph has 2280 edges initially, 62 edges after simplification

total ucounts = 306
nonsolo ucounts = 217[2^46, 3^20, 4^22, 5^14, 6^11, 7^16, 8^12, 9^12, 10^4, 11^6, 12, 13^3, 14^7, 15, 16^5, 17^2, 18^2, 19, 21, 22^6, 23^2, 24^2, 25, 26, 28, 29, 31, 35, 37, 44, 49, 51, 58, 59, 73, 77, 78, 81, 143, 211, 250, 449, 701]
surviving nonsolo ucounts = 5[143, 211, 250, 449, 701]
ids = [74, 250, 26, 266, 5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.535 = ACGAGCCGTTCGAATC-1

using 77 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.544 = ACGAGCCTCAGGCAAG-1

using 143 reads

====================================================================================

graph has 228 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 6, 7, 8, 9, 15, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.548 = ACGAGCCTCATTGCCC-1

using 55 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.549 = ACGAGCCTCCAGAGGA-1

using 5759 reads

====================================================================================

graph has 4950 edges initially, 130 edges after simplification

total ucounts = 759
nonsolo ucounts = 555[2^102, 3^70, 4^55, 5^31, 6^39, 7^29, 8^27, 9^13, 10^18, 11^12, 12^17, 13^16, 14^17, 15^9, 16^18, 17^10, 18^7, 19^12, 20^5, 21^3, 22^2, 23^3, 24^4, 25^4, 26^4, 27^2, 28^3, 29^2, 30^2, 31, 33, 35^2, 36^2, 38^2, 40, 43, 44, 47, 52, 53, 59, 65, 79, 80, 411]
surviving nonsolo ucounts = 1[411]
ids = [80]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.550 = ACGAGCCTCCATGAGT-1

using 38 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.552 = ACGAGCCTCCCTTGCA-1

using 60 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.553 = ACGAGCCTCCGCAGTG-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.556 = ACGAGCCTCCTCGCAT-1

using 24231 reads

====================================================================================

graph has 10940 edges initially, 408 edges after simplification

total ucounts = 731
nonsolo ucounts = 590[2^66, 3^42, 4^32, 5^19, 6^17, 7^14, 8^6, 9^18, 10^10, 11^13, 12^15, 13^8, 14^15, 15^11, 16^9, 17^7, 18^7, 19^7, 20^11, 21^7, 22^8, 23^5, 24^9, 25^3, 26^9, 27^5, 28^6, 29^4, 30^3, 31^10, 32^4, 33^5, 34, 35^5, 36^4, 37^3, 38^2, 39^2, 40^6, 41^7, 42^2, 43, 44^3, 45^2, 46^5, 47^4, 48^4, 49, 50^4, 51, 52^4, 53, 54^5, 55^3, 56^6, 57^7, 58, 59^4, 60^6, 61^2, 62^3, 63^2, 64, 65^3, 67^4, 68^2, 69, 70, 71^2, 72^2, 73^2, 74^3, 75^3, 76^5, 77^2, 79^3, 80^3, 81, 83^4, 84, 86, 87^3, 88^3, 89^5, 90^2, 92, 95, 96, 97, 98, 99^2, 100^3, 105, 106^2, 109^2, 113, 114, 115, 158, 218, 613, 867, 1015, 1236, 1279, 1285, 1608]
surviving nonsolo ucounts = 8[218, 613, 867, 1015, 1236, 1279, 1285, 1608]
ids = [137, 609, 148, 245, 594, 0, 35, 508]

====================================================================================

UMI info for barcode ACGAGCCTCCTCGCAT-1 contig 1 = ACAGTGATCC...
umi AATGCACCTC = 1298 reads: +403 validated
umi ATACTGGTCA = 227 reads: +403 validated
umi ATCGCTCCGT = 862 reads: +403 validated
umi CCGTCACCAC = 1010 reads: +403 validated
umi TAGATCAGGA = 1607 reads: +403 validated
umi TGAAATTCCT = 1292 reads: +122 -10XX +1 -2XX +5 -1XX +1 -3XX +1 -53XX +1 -6XX +2 -4XX +191 invalidated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |674|TRBV7-2|5'UTR| [len=47] (mis=0)
47-394 ==> 0-347 on |675|TRBV7-2|L-REGION+V-REGION| [len=347] (mis=4)
402-450 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=3)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 6 umis using 404 reads
cdr3 = CASSPPASGKETQYF at 377, score = 8 + 7
umis assigned: [35, 137, 148, 245, 508, 594]
of which 6 are surviving nonsolos
reads assigned: 6156
start codons at 47, 156
confident = true

REJECT CONTIGS

TIG 1[bases=523]
0-70 ==> 239-309 on |630|TRBV5-1|5'UTR| [len=309] (mis=0)
20-120 ==> 5783-5883 on segment before TRBV5-2 exon 1 [len=6000] (mis=13)
70-413 ==> 0-343 on |631|TRBV5-1|L-REGION+V-REGION| [len=343] (mis=3)
424-471 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
471-523 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [0, 609, 700]
of which 2 are surviving nonsolos
reads assigned: 1866
start codons at 70, 352, 356
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.558 = ACGAGCCTCCTGCTTG-1

using 37 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.560 = ACGAGCCTCGACCAGC-1

using 46 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.564 = ACGAGCCTCGCTTGTC-1

using 49 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.571 = ACGAGCCTCTACGAGT-1

using 162 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 5, 15, 135]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.572 = ACGAGCCTCTAGAGTC-1

using 34 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 6, 8, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.574 = ACGAGCCTCTCGAGTA-1

using 29 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.575 = ACGAGCCTCTCGGACG-1

using 518 reads

====================================================================================

graph has 198 edges initially, 8 edges after simplification

total ucounts = 31
nonsolo ucounts = 24[2^3, 3, 4^2, 5^2, 6, 11, 13^2, 16, 17^2, 18, 24, 37, 39, 40, 50, 51, 63, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.577 = ACGAGCCTCTCTAAGG-1

using 32 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[6, 10, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.583 = ACGAGCCTCTTAGCCC-1

using 18 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.584 = ACGAGCCTCTTGCATT-1

using 32 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 5, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.586 = ACGAGCCTCTTTAGTC-1

using 22797 reads

====================================================================================

graph has 8668 edges initially, 208 edges after simplification

total ucounts = 943
nonsolo ucounts = 693[2^112, 3^97, 4^77, 5^47, 6^46, 7^32, 8^38, 9^28, 10^20, 11^29, 12^14, 13^18, 14^14, 15^13, 16^16, 17^10, 18^10, 19^9, 20^12, 21^6, 22^4, 23^2, 24, 25^5, 27, 28^2, 30^2, 32, 34, 38, 39^2, 41, 50, 58, 59, 68, 86, 103, 109, 412, 458, 462, 564, 697, 751, 881, 1072, 1123, 1129, 1410, 1538, 2047, 2083, 2168]
surviving nonsolo ucounts = 15[412, 458, 462, 564, 697, 751, 881, 1072, 1123, 1129, 1410, 1538, 2047, 2083, 2168]
ids = [833, 65, 356, 665, 137, 456, 849, 44, 581, 715, ...]

====================================================================================

UMI info for barcode ACGAGCCTCTTTAGTC-1 contig 1 = AGACCAGAAT...
umi AAGACGCTAA = 994 reads: -381X +3 -11XX +2 invalidated
umi ACGGGCCCTA = 694 reads: +397 validated
umi CGCGTACGCG = 410 reads: -381X +3 -11XX +2 invalidated
umi GAGTAGCGCT = 741 reads: +397 validated
umi GGAGGCTCTG = 2174 reads: +397 validated
umi GTACTAATTG = 1116 reads: +397 validated
umi GTGACATGGA = 1296 reads: -381X +3 -11XX +2 invalidated
umi TACCGATTTT = 161 reads: +252 -1 +3 -2 +5 -134 non-validated
umi TATTAACACC = 1913 reads: -381X +3 -11XX +2 invalidated
umi TCAACCAGTC = 1142 reads: +397 validated
umi TTACTATTCT = 862 reads: +397 validated
umi TTACTGGTCT = 1527 reads: +397 validated
umi TTTAATACCA = 2056 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=496]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-384 ==> 0-337 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=0)
391-444 ==> 0-53 on |541|TRBJ1-6|J-REGION| [len=53] (mis=2)
444-496 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 8 umis using 816 reads
cdr3 = CASKVSDYNSPLHF at 374, score = 9 + 9
umis assigned: [44, 137, 356, 456, 535, 581, 614, 665, 704, 715] and 3 others
of which 13 are surviving nonsolos
reads assigned: 14944
start codons at 47, 146, 169, 189, 197, 239, 273
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.591 = ACGAGGAAGAGCTTCT-1

using 34 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.595 = ACGAGGAAGATCGGGT-1

using 58 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.602 = ACGAGGAAGGAGCGTT-1

using 57 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.604 = ACGAGGAAGGTGATAT-1

using 53 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.608 = ACGAGGAAGTATGACA-1

using 103 reads

====================================================================================

graph has 128 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2, 4, 7, 11, 15, 17, 18, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.611 = ACGAGGAAGTGCCATT-1

using 110 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[109]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.614 = ACGAGGAAGTGGCACA-1

using 52 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.615 = ACGAGGAAGTGTCTCA-1

using 57 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.618 = ACGAGGACAAAGTGCG-1

using 27 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.620 = ACGAGGACAACACCCG-1

using 61 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.622 = ACGAGGACAACTTGAC-1

using 163 reads

====================================================================================

graph has 46 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[66, 97]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.623 = ACGAGGACAAGACACG-1

using 3690 reads

====================================================================================

graph has 3445 edges initially, 100 edges after simplification

total ucounts = 427
nonsolo ucounts = 299[2^48, 3^34, 4^24, 5^20, 6^16, 7^15, 8^16, 9^11, 10^8, 11^13, 12^7, 13^8, 14^10, 15^3, 16^9, 17^2, 18^5, 19^5, 20^2, 21^3, 22^3, 23, 24^2, 25^3, 26, 27^2, 29^2, 32, 33, 34, 35, 36^2, 37, 38^2, 41^2, 42, 43, 44, 45^2, 52, 60, 62, 63, 64, 70, 73^2, 84, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.627 = ACGAGGACAATCCGAT-1

using 77 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.630 = ACGAGGACACAGATTC-1

using 136 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[136]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.638 = ACGAGGACACGGTGTC-1

using 54 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.645 = ACGAGGACAGATCGGA-1

using 25 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.646 = ACGAGGACAGATGAGC-1

using 87 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.651 = ACGAGGACAGGAATCG-1

using 30 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.654 = ACGAGGACAGGTCGTC-1

using 61 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.658 = ACGAGGACATACGCCG-1

using 5591 reads

====================================================================================

graph has 3193 edges initially, 64 edges after simplification

total ucounts = 305
nonsolo ucounts = 204[2^41, 3^16, 4^11, 5^17, 6^15, 7^14, 8^6, 9^8, 10^12, 11^8, 12^3, 13^5, 14^4, 15^6, 16^7, 17^2, 18, 19^2, 20^2, 22^2, 25^2, 26, 28^2, 30^3, 31, 33, 46, 52, 55^2, 60^2, 61, 69, 71, 75, 109, 3183]
surviving nonsolo ucounts = 1[3183]
ids = [101]

====================================================================================

REJECT CONTIGS

TIG 1[bases=425]
22-68 ==> 0-46 on |545|TRBJ2-2P|J-REGION| [len=46] (mis=0)
68-172 ==> 0-104 on segment before TRBJ2-3 exon 1 [len=104] (mis=0)
221-323 ==> 0-102 on segment before TRBJ2-4 exon 1 [len=102] (mis=0)
373-425 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [101, 263, 297]
of which 1 are surviving nonsolos
reads assigned: 3151
start codons at 82
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.659 = ACGAGGACATATGCTG-1

using 98 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.665 = ACGAGGACATTTGCTT-1

using 106 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[105]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.669 = ACGAGGAGTACGCACC-1

using 76 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3^2, 5^2, 7, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.672 = ACGAGGAGTAGAAGGA-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.681 = ACGAGGAGTCATCGGC-1

using 72826 reads

====================================================================================

graph has 15619 edges initially, 100 edges after simplification

total ucounts = 393
nonsolo ucounts = 239[2^50, 3^23, 4^18, 5^11, 6^17, 7^8, 8^9, 9^5, 10^6, 11^9, 12^6, 13^5, 14^7, 15^2, 16^4, 17^5, 18^2, 19^3, 20, 21, 22^5, 23^3, 24^2, 26, 27^2, 28^2, 32, 34, 63, 71, 74, 87, 90, 125, 169, 254, 272, 973, 1392, 1415, 1546, 1614, 1638, 1714, 1832, 1915, 1920, 1999, 2250, 2456, 2719, 4671, 4696, 5872, 6179, 6679, 7572, 8708]
surviving nonsolo ucounts = 21[973, 1392, 1415, 1546, 1614, 1638, 1714, 1832, 1915, 1920, 1999, 2250, 2456, 2719, 4671, 4696, 5872, 6179, 6679, 7572, 8708]
ids = [155, 77, 180, 47, 376, 278, 286, 158, 173, 181, ...]

====================================================================================

UMI info for barcode ACGAGGAGTCATCGGC-1 contig 1 = AGAGAAGGTG...
umi AACGCGCTTA = 6861 reads: +31 -3XX +3 -5XX +1 -3XX +2 -3XX +1 -4XX +1 -74X +1 -9XX +1 -2XX +1 -2XX +1 -3XX +1 -1XX +235 invalidated
umi ACTCTCCGTG = 1545 reads: -2XX +386 invalidated
umi CCGTCCATAT = 2227 reads: +388 validated
umi CGGCAACAGA = 1833 reads: +388 validated
umi CTATCTTATG = 2268 reads: +381 -7X invalidated
umi CTCCCAAGAT = 2305 reads: -353 +1 -3X +6 -1XX +1 -1XX +2 -1XX +1 -3XX +4 -1XX +8 -1XX +1 invalidated
umi CTCCTTACAT = 1384 reads: +388 validated
umi CTCGTGTTTG = 1917 reads: +388 validated
umi GCGTGCACTT = 2726 reads: -5X +1 -2XX +380 invalidated
umi GGGGCTGGGA = 2002 reads: +388 validated
umi GTGTTCCGAA = 1625 reads: +388 validated
umi TAAATCTGCT = 1654 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=470]
0-30 ==> 48-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
30-365 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
368-418 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=2)
418-470 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 11 umis using 1801 reads
cdr3 = CSARAGPYNEQFF at 351, score = 7 + 8
umis assigned: [9, 47, 139, 158, 173, 179, 180, 181, 236, 249] and 2 others
of which 12 are surviving nonsolos
reads assigned: 27387
start codons at 30, 168, 204, 210, 223, 283, 376
confident = true

REJECT CONTIGS

TIG 1[bases=411]
20-301 ==> 5719-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
309-359 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=4)
359-411 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [60, 77, 102, 113, 216, 266, 302, 376]
of which 8 are surviving nonsolos
reads assigned: 37650
start codons at 178, 188, 262, 309, 335
confident = false
did not find CDR3

TIG 2[bases=506]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=7)
421-464 ==> 17-60 on |400|TRAJ16|J-REGION| [len=60] (mis=1)
464-506 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [155]
of which 1 are surviving nonsolos
reads assigned: 942
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414, 445
confident = false
frameshifted full length transcript of length 506
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.682 = ACGAGGAGTCCAGTTA-1

using 171 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[171]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.683 = ACGAGGAGTCGACTAT-1

using 38 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.689 = ACGAGGAGTCTCCATC-1

using 34 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.692 = ACGAGGAGTCTTGTCC-1

using 91 reads

====================================================================================

graph has 60 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 5, 6, 9, 15, 18, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.699 = ACGAGGAGTGTAAGTA-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.701 = ACGAGGAGTGTTGAGG-1

using 21 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.702 = ACGAGGAGTTAAGGGC-1

using 26 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.714 = ACGAGGAGTTTCGCTC-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.715 = ACGAGGAGTTTGACTG-1

using 584 reads

====================================================================================

graph has 262 edges initially, 8 edges after simplification

total ucounts = 21
nonsolo ucounts = 15[2^2, 3, 5, 6, 9^3, 17, 44, 51, 53, 113, 116, 139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.717 = ACGAGGAGTTTGTTTC-1

using 5005 reads

====================================================================================

graph has 5536 edges initially, 188 edges after simplification

total ucounts = 666
nonsolo ucounts = 441[2^87, 3^57, 4^43, 5^29, 6^24, 7^23, 8^16, 9^15, 10^10, 11^16, 12^11, 13^5, 14^11, 15^4, 16^5, 17^3, 18^5, 19^5, 20^7, 21^4, 22^3, 23^4, 24^2, 25, 26^3, 27^4, 28^3, 29^2, 30^2, 31, 32, 33^2, 34, 35^2, 37^2, 38^3, 39^2, 40^2, 41^3, 42^2, 43^2, 46, 47, 48, 51, 54^2, 55, 58, 60, 62, 63, 65, 70, 99]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.719 = ACGAGGATCAAACGGG-1

using 146 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 18
nonsolo ucounts = 13[2, 3^2, 4^3, 6, 7, 8, 14, 16, 19, 51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.721 = ACGAGGATCACAACGT-1

using 56 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 4, 5, 7, 8, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.724 = ACGAGGATCACGATGT-1

using 35 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.726 = ACGAGGATCAGAGACG-1

using 36 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[13, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.727 = ACGAGGATCAGGTAAA-1

using 21 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.730 = ACGAGGATCATCACCC-1

using 33 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.731 = ACGAGGATCATCGATG-1

using 86 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.734 = ACGAGGATCATTGCGA-1

using 36 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 5, 7, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.737 = ACGAGGATCCAGTAGT-1

using 79 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[79]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.742 = ACGAGGATCCTAAGTG-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.744 = ACGAGGATCGCAAACT-1

using 43 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.746 = ACGAGGATCGGACAAG-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.748 = ACGAGGATCGTACCGG-1

using 52 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.749 = ACGAGGATCGTTGACA-1

using 127 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[127]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.751 = ACGAGGATCTAACGGT-1

using 74 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.753 = ACGAGGATCTACGAGT-1

using 49 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[3, 12, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.754 = ACGAGGATCTAGAGTC-1

using 139 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3^2, 5, 13, 18, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.756 = ACGAGGATCTCCAGGG-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.757 = ACGAGGATCTCCCTGA-1

using 37 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.759 = ACGAGGATCTCTTATG-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.761 = ACGAGGATCTGCGTAA-1

using 33 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.762 = ACGAGGATCTGTCTCG-1

using 8714 reads

====================================================================================

graph has 7403 edges initially, 214 edges after simplification

total ucounts = 1041
nonsolo ucounts = 765[2^139, 3^84, 4^67, 5^50, 6^55, 7^44, 8^29, 9^26, 10^23, 11^28, 12^20, 13^18, 14^17, 15^16, 16^15, 17^19, 18^10, 19^11, 20^10, 21^13, 22^5, 23^4, 24^3, 25^3, 26^5, 27^3, 28^3, 29^3, 30^6, 31^4, 32^3, 33, 34^2, 35, 36, 37^2, 38^2, 39^2, 41, 42^2, 43, 44, 50, 53, 58, 59^2, 60, 65, 73, 87, 104, 174, 185, 600]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.765 = ACGAGGATCTTGGGTA-1

using 433 reads

====================================================================================

graph has 268 edges initially, 10 edges after simplification

total ucounts = 21
nonsolo ucounts = 16[2^2, 3^2, 6^2, 9, 11, 12^2, 45, 51, 59, 60, 65, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.770 = ACGATACAGAAGGGTA-1

using 80000 reads

====================================================================================

graph has 25392 edges initially, 556 edges after simplification

total ucounts = 1438
nonsolo ucounts = 1073[2^127, 3^101, 4^67, 5^74, 6^59, 7^49, 8^39, 9^41, 10^26, 11^33, 12^27, 13^31, 14^25, 15^32, 16^23, 17^18, 18^14, 19^16, 20^18, 21^12, 22^10, 23^16, 24^14, 25^9, 26^8, 27^8, 28^14, 29^12, 30^10, 31^14, 32^6, 33^6, 34^5, 35^6, 36^3, 37^5, 39, 40^2, 41^3, 42^2, 43, 44, 45^2, 46, 47, 50, 52, 53, 54^2, 55, 56, 57, 58^3, 59, 62^3, 63^2, 64^2, 66, 69^2, 70, 73, 75, 76, 77, 78, 80, 83, 88, 89, 90, 93^2, 98, 100, 108, 109, 119, 128, 132, 157, 183, 473, 814, 854, 872, 1008, 1047, 1074, 1144, 1153, 1159, 1219, 1230, 1239, 1265, 1312, 1337, 1369, 1388, 1401, 1448, 1489, 1500, 1501, 1674, 1730, 1789, 1890, 1984, 2006, 2030, 2166, 2288, 2431, 2479, 2538, 6014, 6512]
surviving nonsolo ucounts = 39[13, 37, 473, 814, 854, 872, 1008, 1047, 1074, 1144, 1153, 1159, 1219, 1230, 1239, 1265, 1312, 1337, 1369, 1388, 1401, 1448, 1489, 1500, 1501, 1674, 1730, 1789, 1890, 1984, 2006, 2030, 2166, 2288, 2431, 2479, 2538, 6014, 6512]
ids = [51, 620, 535, 678, 182, 835, 944, 598, 356, 739, ...]

====================================================================================

UMI info for barcode ACGATACAGAAGGGTA-1 contig 1 = AGAATGACGC...
umi AACTAAGGGA = 13 reads: -26 +232 -143 +5 non-validated
umi ATCACCCAGC = 1159 reads: +406 validated
umi ATTGCAGTAT = 1509 reads: +406 validated
umi CATGAACAGT = 1354 reads: +406 validated
umi CCGTTGGGCG = 1234 reads: +406 validated
umi CTACATTAGG = 37 reads: -38X +1 -1XX +1 -1XX +1 -2XX +261 -1 +10 -39 +1 -3 +5 -2 +1 -3 +5 -1 +5 -3 +8 -1 +12 invalidated
umi GCCCATGCAG = 1266 reads: +406 validated
umi TGCGTAGCAC = 1391 reads: +406 validated
umi TGTCTGGGCA = 2526 reads: -44X +2 -1XX +359 invalidated

UMI info for barcode ACGATACAGAAGGGTA-1 contig 2 = GTCATCCCTC...
umi AACCCCTCCA = 2307 reads: +394 validated
umi ACATACACCA = 1449 reads: +394 validated
umi ACTGCATCCG = 3 reads: -393X +1 invalidated
umi AGCTGCGTGT = 2440 reads: +394 validated
umi AGGCGGGTAG = 1400 reads: +394 validated
umi ATTTATTTCG = 2038 reads: +394 validated
umi CATTGGCCTA = 1716 reads: -310 +1 -7XX +2 -5XX +1 -8XX +2 -9XX +49 invalidated
umi CTCGAGTCAG = 1255 reads: +394 validated
umi GAGTCTCCTC = 1990 reads: +394 validated
umi GTCAAGCCAT = 6532 reads: -149 +1 -1XX +1 -2XX +240 invalidated
umi GTCAGCTTGG = 6174 reads: +102 -3XX +1 -2XX +2 -1XX +1 -1XX +2 -2XX +1 -4XX +2 -2XX +3 -4XX +2 -90X +1 -3X +1 -3XX +1 -1XX +1 -2XX +3 -4XX +1 -1XX +2 -4XX +1 -1XX +139 invalidated
umi GTGCGTCTTG = 1006 reads: +394 validated
umi TCCACCCACC = 1228 reads: +394 validated
umi TCGCAGTCCA = 1 reads: -84 +56 -254 non-validated
umi TCGCTGCCCA = 1686 reads: +394 validated
umi TCGCTGTTCT = 2165 reads: +394 validated
umi TGGTAACCTG = 1324 reads: +394 validated
umi TTAATCCTTT = 1158 reads: +394 validated
umi TTCACAGATT = 1286 reads: +394 validated
umi TTTAAACGTT = 1495 reads: +394 validated

UMI info for barcode ACGATACAGAAGGGTA-1 contig 3 = TGGGGAGAGT...
umi AAAAAACCAA = 2492 reads: +394 validated
umi ACTTTTCGCG = 3 reads: -379X +1 -3XX +3 -1XX +2 -1XX +2 -1XX +1 invalidated
umi AGTCTATGAT = 1909 reads: +394 validated
umi CAAGCTGACG = 1064 reads: +394 validated
umi CCCATATTGC = 1738 reads: +394 validated
umi CCTATATCCT = 472 reads: +394 validated
umi CGGTATTTTG = 1057 reads: +394 validated
umi CTGCGACCCG = 58 reads: -362 +4 -1X +4 -1X +3 -4X +1 -3XX +3 -1XX +2 -1XX +2 -1XX +1 invalidated
umi GAGCAACTCA = 5 reads: -379X +1 -3XX +3 -1XX +2 -1XX +2 -1XX +1 invalidated
umi GCTATTTGCT = 872 reads: +394 validated
umi GTGTTGCACC = 1512 reads: +394 validated
umi TACCCTGTGA = 2014 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=538]
0-80 ==> 0-80 on |651|TRBV6-2|5'UTR| [len=80] (mis=0)
80-424 ==> 0-344 on |652|TRBV6-2|L-REGION+V-REGION| [len=344] (mis=0)
435-486 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=5)
486-538 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 657 reads
cdr3 = CASSYSRTAVIPGELFF at 407, score = 9 + 9
umis assigned: [51, 286, 326, 429, 526, 620, 811, 1245, 1285]
of which 9 are surviving nonsolos
reads assigned: 10326
start codons at 3, 38, 80, 138, 191, 215, 222, 230, 257, 321, 330
confident = true

TIG 2[bases=524]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
422-472 ==> 0-50 on |543|TRBJ2-1|J-REGION| [len=50] (mis=1)
472-524 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 17 umis using 2576 reads
cdr3 = CSARVGTPSYNEQFF at 399, score = 7 + 8
umis assigned: [45, 118, 173, 215, 220, 331, 447, 654, 748, 923] and 10 others
of which 18 are surviving nonsolos
reads assigned: 37872
start codons at 21, 78, 216, 252, 258, 271, 331, 430
confident = true

TIG 3[bases=520]
84-420 ==> 0-336 on |481|TRAV21|L-REGION+V-REGION| [len=336] (mis=1)
415-478 ==> 0-63 on |444|TRAJ58|J-REGION| [len=63] (mis=4)
478-520 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 9 umis using 844 reads
cdr3 = CAVRETSGSRLTF at 408, score = 9 + 9
umis assigned: [0, 182, 237, 356, 483, 535, 598, 678, 739, 835] and 2 others
of which 12 are surviving nonsolos
reads assigned: 12966
start codons at 84, 128, 328
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.771 = ACGATACAGACACTAA-1

using 54 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.773 = ACGATACAGACCTAGG-1

using 39 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.774 = ACGATACAGAGCTTCT-1

using 61 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[8, 23, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.775 = ACGATACAGAGTTGGC-1

using 63 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[10, 18, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.776 = ACGATACAGATAGCAT-1

using 198 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[198]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.784 = ACGATACAGCACGCCT-1

using 50 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3, 4, 5, 6, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.786 = ACGATACAGCATCATC-1

using 24 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.793 = ACGATACAGCTGAACG-1

using 261 reads

====================================================================================

graph has 174 edges initially, 10 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[3, 6, 10, 13, 15, 29^2, 43, 46, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.794 = ACGATACAGCTGGAAC-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.796 = ACGATACAGGACACCA-1

using 25 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.797 = ACGATACAGGACCACA-1

using 4895 reads

====================================================================================

graph has 1052 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[4, 7, 4878]
surviving nonsolo ucounts = 1[4878]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.798 = ACGATACAGGACGAAA-1

using 138 reads

====================================================================================

graph has 88 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[6^2, 11, 17, 19, 25, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.801 = ACGATACAGGCTACGA-1

using 109 reads

====================================================================================

graph has 116 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[3^2, 31, 35, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.802 = ACGATACAGGGCATGT-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.803 = ACGATACAGGTGACCA-1

using 39 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 16, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.806 = ACGATACAGTATCTCG-1

using 426 reads

====================================================================================

graph has 244 edges initially, 10 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 5, 9, 12, 13, 54, 56, 60, 93, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.813 = ACGATACAGTGGGCTA-1

using 26 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.814 = ACGATACAGTGTTAGA-1

using 164 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3, 4, 9, 26, 47, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.815 = ACGATACAGTTCGCGC-1

using 48 reads

====================================================================================

graph has 73 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.816 = ACGATACAGTTTAGGA-1

using 81 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.817 = ACGATACAGTTTCCTT-1

using 87 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.819 = ACGATACCAAACTGCT-1

using 61 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.823 = ACGATACCAATAGCAA-1

using 111 reads

====================================================================================

graph has 72 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2^2, 3, 4^2, 7, 18, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.824 = ACGATACCAATCTACG-1

using 41 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[5, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.825 = ACGATACCAATGTTGC-1

using 106 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.826 = ACGATACCAATTCCTT-1

using 32 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.827 = ACGATACCACAGAGGT-1

using 1813 reads

====================================================================================

graph has 949 edges initially, 14 edges after simplification

total ucounts = 66
nonsolo ucounts = 57[2^3, 3^8, 4^3, 5, 7^2, 8^2, 9, 10^2, 11^4, 14^2, 15, 16, 18, 19, 20, 21, 24, 25^2, 40, 41, 42, 47^2, 50, 51, 52, 70, 73, 76, 79, 85, 87^2, 93, 95, 97^2, 134]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.834 = ACGATACCACGCTTTC-1

using 161 reads

====================================================================================

graph has 130 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3, 4, 6, 8, 16, 53, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.836 = ACGATACCACTTAAGC-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.839 = ACGATACCAGACTCGC-1

using 61 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 4, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.843 = ACGATACCAGCTTCGG-1

using 77 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.844 = ACGATACCAGGGCATA-1

using 1507 reads

====================================================================================

graph has 534 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 1501]
surviving nonsolo ucounts = 1[1501]
ids = [3]

====================================================================================

UMI info for barcode ACGATACCAGGGCATA-1 contig 1 = AGTGGTCACA...
umi TCTTCACCAT = 1514 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=501]
0-49 ==> 29-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
49-384 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=4)
400-449 ==> 0-49 on |546|TRBJ2-3|J-REGION| [len=49] (mis=0)
449-501 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 80 reads
cdr3 = CSAPVLQREWSTDTQYF at 370, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 1478
start codons at 49, 187, 223, 229, 242, 302
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.845 = ACGATACCATACGCCG-1

using 7975 reads

====================================================================================

graph has 1454 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 4[2, 4, 24, 7937]
surviving nonsolo ucounts = 1[7937]
ids = [10]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.847 = ACGATACCATATGGTC-1

using 28 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.848 = ACGATACCATCGGTTA-1

using 64 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.849 = ACGATACCATGACGGA-1

using 24 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.850 = ACGATACCATGCCTAA-1

using 72 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[4, 5, 8, 10, 13, 30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.855 = ACGATACCATTCCTGC-1

using 94 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 12^2, 26, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.856 = ACGATACCATTGGTAC-1

using 45 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.860 = ACGATACGTATGAATG-1

using 36 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.863 = ACGATACGTCTCCACT-1

using 57 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.864 = ACGATACGTCTGATCA-1

using 64 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.866 = ACGATACGTGCCTTGG-1

using 173 reads

====================================================================================

graph has 114 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 7, 28, 33, 47, 52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.867 = ACGATACGTGCGGTAA-1

using 74 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.869 = ACGATACGTTATCGGT-1

using 42 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.870 = ACGATACGTTCAGGCC-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.872 = ACGATACGTTCCTCCA-1

using 68 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 8, 9, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.875 = ACGATACGTTCGTCTC-1

using 63 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 6.880 = ACGATACTCAACTCTT-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.17
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk06-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk06-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

100.195 seconds used processing barcodes, peak mem = 0.34
