[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.04 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk05-ucc6a7a72e6 /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk05-ucc6a7a72e6/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_T_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk05.ucc6a7a72e6
n50_n50_rpu = 2159

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.0 = ACATCAGCAGTGACAG-1

using 1890 reads

====================================================================================

graph has 702 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 9, 1871]
surviving nonsolo ucounts = 1[1871]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=380]
0-264 ==> 69-333 on |560|TRBV10-3|L-REGION+V-REGION| [len=344] (mis=1)
281-328 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=1)
328-380 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
cdr3 = CAVQRGRDSYEQYF at 258, score = 10 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1600
start codons at 53, 81, 109, 136, 172
confident = false
not full
VJ delta = 16
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.2 = ACATCAGCATAAGACA-1

using 64 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.4 = ACATCAGCATCACCCT-1

using 74 reads

====================================================================================

graph has 84 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 3^2, 4^2, 25, 28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.6 = ACATCAGCATCAGTAC-1

using 16 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.7 = ACATCAGCATCCGCGA-1

using 292 reads

====================================================================================

graph has 294 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 16[2^2, 3, 4^2, 5, 7^2, 8, 9^2, 10, 24, 32, 72, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.8 = ACATCAGCATCTCGCT-1

using 53 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.9 = ACATCAGCATGCCACG-1

using 60 reads

====================================================================================

graph has 44 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[12, 14, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.11 = ACATCAGCATGGTCAT-1

using 175 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 163]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.12 = ACATCAGCATGTAGTC-1

using 8 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.15 = ACATCAGCATTTGCCC-1

using 292 reads

====================================================================================

graph has 174 edges initially, 8 edges after simplification

total ucounts = 16
nonsolo ucounts = 11[2, 4, 11, 12, 14, 18, 21, 22, 45, 47, 91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.16 = ACATCAGCATTTGCTT-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.17 = ACATCAGGTAAACACA-1

using 68 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.19 = ACATCAGGTAATAGCA-1

using 43 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.20 = ACATCAGGTACAGCAG-1

using 59 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[59]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.22 = ACATCAGGTACCATCA-1

using 7010 reads

====================================================================================

graph has 6037 edges initially, 232 edges after simplification

total ucounts = 791
nonsolo ucounts = 580[2^100, 3^47, 4^40, 5^30, 6^39, 7^30, 8^21, 9^24, 10^20, 11^21, 12^18, 13^17, 14^10, 15^13, 16^6, 17^11, 18^9, 19^5, 20^11, 21^11, 22^13, 23^9, 24^5, 25^8, 26^7, 27^7, 28^10, 29^4, 30^5, 31^5, 32, 33^2, 34^3, 37, 38, 39, 41^2, 42, 43, 46^2, 47, 54, 59, 62, 66, 71, 86, 109, 114]
surviving nonsolo ucounts = 11[3^4, 4, 6^2, 8, 9, 10, 27]
ids = [235, 261, 549, 751, 382, 591, 679, 456, 211, 95, ...]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.25 = ACATCAGGTACTTCTT-1

using 68 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.29 = ACATCAGGTCACACGC-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.31 = ACATCAGGTCAGTGGA-1

using 20 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.33 = ACATCAGGTCCCTTGT-1

using 67 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.35 = ACATCAGGTCGAATCT-1

using 11 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.37 = ACATCAGGTCTCAACA-1

using 54 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.40 = ACATCAGGTCTCTTAT-1

using 48 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 6[2^2, 3, 5, 8, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.41 = ACATCAGGTCTCTTTA-1

using 70066 reads

====================================================================================

graph has 33646 edges initially, 1199 edges after simplification

total ucounts = 3305
nonsolo ucounts = 2546[2^342, 3^232, 4^188, 5^165, 6^139, 7^123, 8^90, 9^117, 10^81, 11^75, 12^86, 13^66, 14^53, 15^55, 16^51, 17^50, 18^50, 19^43, 20^43, 21^54, 22^48, 23^39, 24^31, 25^36, 26^30, 27^22, 28^30, 29^30, 30^19, 31^14, 32^15, 33^7, 34^9, 35^13, 36^11, 37^9, 38^3, 39^3, 40^5, 41^6, 42^7, 43, 44^2, 45^5, 46^6, 47, 49^4, 50^2, 51^2, 53, 55^3, 56^2, 57^2, 63, 64, 65, 66^2, 71, 75^2, 79, 84, 93, 94, 116, 123, 207, 870, 1192, 1259, 2147, 2197, 2869, 4293, 6825, 8170, 8872]
surviving nonsolo ucounts = 10[207, 870, 1192, 2147, 2197, 2869, 4293, 6825, 8170, 8872]
ids = [456, 1976, 823, 1170, 2406, 1747, 1006, 3264, 2200, 274]

====================================================================================

REJECT CONTIGS

TIG 1[bases=515]
6-88 ==> 31-113 on segment before TRBV21-1 exon 2 [len=113] (mis=0)
416-463 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=5)
463-515 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
umis assigned: [274, 823, 1006, 1976, 2200, 2406, 3264]
of which 7 are surviving nonsolos
reads assigned: 26668
start codons at 156, 187, 247, 280, 299
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.43 = ACATCAGGTGAACCTT-1

using 35 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.44 = ACATCAGGTGATGTCT-1

using 19 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.48 = ACATCAGGTGGTACAG-1

using 35 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.49 = ACATCAGGTGGTTTCA-1

using 30 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.50 = ACATCAGGTGTAATGA-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.51 = ACATCAGGTGTCAATC-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.52 = ACATCAGGTGTTGAGG-1

using 36 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.55 = ACATCAGGTTACGTCA-1

using 40 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.56 = ACATCAGGTTATCCGA-1

using 35 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.57 = ACATCAGGTTATTCTC-1

using 129 reads

====================================================================================

graph has 146 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[3^2, 5^2, 6^2, 7, 10, 11, 12, 27, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.60 = ACATCAGGTTCTCATT-1

using 101 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[101]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.63 = ACATCAGGTTGATTGC-1

using 38 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 6, 8, 9, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.65 = ACATCAGGTTGGTAAA-1

using 1597 reads

====================================================================================

graph has 1024 edges initially, 22 edges after simplification

total ucounts = 75
nonsolo ucounts = 60[2^8, 3^4, 4^4, 5^3, 6, 7^3, 9^3, 10^2, 11^2, 14^3, 16^3, 19, 21, 22^2, 23, 29^2, 30, 33^3, 34, 48, 55, 66, 74, 82, 84, 95, 98, 99, 100, 102, 106]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.70 = ACATCAGTCACAAACC-1

using 35994 reads

====================================================================================

graph has 18282 edges initially, 514 edges after simplification

total ucounts = 1656
nonsolo ucounts = 1212[2^181, 3^116, 4^73, 5^73, 6^76, 7^57, 8^62, 9^58, 10^37, 11^36, 12^27, 13^32, 14^40, 15^29, 16^21, 17^21, 18^20, 19^24, 20^16, 21^19, 22^20, 23^19, 24^6, 25^10, 26^6, 27^16, 28^7, 29^10, 30^6, 31^8, 32^2, 33^6, 34^3, 35^6, 36^5, 37^4, 38^2, 39, 40^2, 41^3, 42^5, 43, 44^2, 46^3, 48, 49^2, 51^3, 53^3, 54, 55^2, 57, 58, 59, 60^3, 63^2, 65, 81, 83, 85, 92, 102, 108, 123, 130, 218, 251, 280, 434, 926, 1012, 1159, 1253, 1254, 3586, 4153, 6612]
surviving nonsolo ucounts = 14[60, 123, 130, 251, 280, 434, 926, 1012, 1159, 1253, 1254, 3586, 4153, 6612]
ids = [1455, 166, 772, 583, 1117, 367, 172, 324, 1255, 907, ...]

====================================================================================

UMI info for barcode ACATCAGTCACAAACC-1 contig 1 = GAGAGAATGG...
umi ACCAGCGGCG = 929 reads: +353 -27 +17 non-validated
umi ATCCAGCTAC = 439 reads: +397 validated
umi TGGCTTTATA = 62 reads: +362 -13 +22 non-validated

UMI info for barcode ACATCAGTCACAAACC-1 contig 2 = TTCCTCTGCT...
umi ACATTGAGGC = 84 reads: -372X +5 -2XX +3 invalidated
umi ACGCGGAGGA = 3598 reads: +382 validated
umi AGTGATGACG = 1023 reads: +382 validated
umi AGTTTGCTTA = 1193 reads: -7X +375 invalidated
umi CCGACCCGAG = 185 reads: -371 +1 -5XX +2 -1XX +2 invalidated
umi CGACCGCCAA = 2663 reads: -259X +123 invalidated
umi GAAAGTTATC = 5222 reads: -351X +2 -1XX +2 -4XX +2 -1XX +3 -2XX +1 -1XX +1 -2XX +1 -1XX +2 -2XX +1 -1XX +1 invalidated
umi GAAATGTCAG = 105 reads: -371X +1 -5XX +2 -1XX +2 invalidated
umi TAAAAATTCA = 240 reads: -370 +1 -1XX +5 -2XX +3 invalidated
umi TCACATCCAC = 1132 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=633]
194-531 ==> 0-337 on |458|TRAV12-1|L-REGION+V-REGION| [len=337] (mis=2)
531-591 ==> 0-60 on |397|TRAJ12|J-REGION| [len=60] (mis=1)
591-633 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 0 umis using 0 reads
cdr3 = CVVTPMDSSYKLIF at 518, score = 7 + 9
umis assigned: [172, 367, 1455]
of which 3 are surviving nonsolos
reads assigned: 1403
start codons at 6, 96, 133, 191, 194, 291, 398, 420, 426, 533
confident = true

TIG 2[bases=546]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-440 ==> 0-328 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=0)
453-494 ==> 7-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=0)
494-546 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 5 umis using 1300 reads
cdr3 = CAFSYGTGYTF at 433, score = 7 + 9
umis assigned: [166, 211, 324, 334, 583, 632, 769, 772, 1117, 1255]
of which 10 are surviving nonsolos
reads assigned: 15235
start codons at 109, 112, 180, 446
confident = true

REJECT CONTIGS

TIG 1[bases=512]
0-48 ==> 63-111 on |494|TRAV29/DV5|5'UTR| [len=111] (mis=1)
48-406 ==> 0-358 on |495|TRAV29/DV5|L-REGION+V-REGION| [len=358] (mis=2)
408-470 ==> 0-62 on |415|TRAJ3|J-REGION| [len=62] (mis=4)
470-512 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CTSVQQALPYSSASKIIF at 385, score = 3 + 9
umis assigned: [907, 1008]
of which 1 are surviving nonsolos
reads assigned: 1243
start codons at 48, 54, 121, 124, 210, 227, 295, 301
confident = false
frameshifted full length transcript of length 512
VJ delta = 32
not full
not full
now this is a cell
paired!

AAGCTCAGTGATTCAGCCACCTACCTCTGTGTGGTGACCCCGATGGATAGCAGCTATAAATTGATCTTCGGGAGTGGGACCAGACTGCTGGTCAGGCCTG <==> TCTAAGAAGCTCCTTCTCAGTGACTCTGGCTTCTATCTCTGTGCCTTCTCGTATGGAACTGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.74 = ACATCAGTCAGCATGT-1

using 65 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.75 = ACATCAGTCAGCTCTC-1

using 527 reads

====================================================================================

graph has 334 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 20[2, 3^3, 7, 9, 10, 13, 15^2, 20, 23^2, 38, 41, 42, 45, 51, 65, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.77 = ACATCAGTCAGGATCT-1

using 118 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.80 = ACATCAGTCAGTGTTG-1

using 64 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.81 = ACATCAGTCATAACCG-1

using 39 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[11, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.82 = ACATCAGTCATACGGT-1

using 11300 reads

====================================================================================

graph has 9072 edges initially, 346 edges after simplification

total ucounts = 1130
nonsolo ucounts = 865[2^132, 3^87, 4^71, 5^52, 6^38, 7^38, 8^27, 9^23, 10^25, 11^30, 12^31, 13^20, 14^11, 15^24, 16^12, 17^19, 18^20, 19^14, 20^13, 21^11, 22^16, 23^13, 24^10, 25^14, 26^8, 27^9, 28^12, 29^8, 30^5, 31^7, 32^6, 33^6, 34^6, 35^4, 36^2, 37^3, 38^2, 41^3, 43^2, 44^2, 45^2, 46^2, 48, 49, 50, 51^3, 52^2, 54, 55^2, 59, 60, 62^3, 66, 67, 71, 72, 73, 79, 81, 96, 114]
surviving nonsolo ucounts = 6[2, 3^2, 4, 12, 19]
ids = [748, 366, 831, 442, 241, 639]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.86 = ACATCAGTCATTATCC-1

using 90 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.95 = ACATCAGTCCATTCTA-1

using 52 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.100 = ACATCAGTCCTATGTT-1

using 50 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.101 = ACATCAGTCCTCAACC-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.103 = ACATCAGTCCTGCAGG-1

using 1896 reads

====================================================================================

graph has 1700 edges initially, 64 edges after simplification

total ucounts = 240
nonsolo ucounts = 158[2^21, 3^18, 4^18, 5^15, 6^6, 7^10, 8^8, 9^4, 10^7, 11^2, 12^5, 13^6, 14, 15^4, 16^2, 17^4, 18^2, 19, 20^3, 22^3, 24, 25, 26^2, 27^2, 28, 33, 35, 40, 41, 44, 60, 61, 63, 66, 68, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.107 = ACATCAGTCGAACTGT-1

using 31 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.108 = ACATCAGTCGACCAGC-1

using 332 reads

====================================================================================

graph has 226 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[3, 4, 5, 6, 8, 12, 18^2, 19, 22, 23, 32, 64, 95]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.110 = ACATCAGTCGGAGCAA-1

using 1400 reads

====================================================================================

graph has 396 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[5, 27, 30, 1337]
surviving nonsolo ucounts = 1[1337]
ids = [0]

====================================================================================

UMI info for barcode ACATCAGTCGGAGCAA-1 contig 1 = AGACCAGAAT...
umi AGTAGTCATG = 1340 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=502]
0-47 ==> 0-47 on |616|TRBV3-1|5'UTR| [len=47] (mis=0)
47-391 ==> 0-344 on |617|TRBV3-1|L-REGION+V-REGION| [len=344] (mis=2)
402-450 ==> 0-48 on |548|TRBJ2-5|J-REGION| [len=48] (mis=1)
450-502 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 1 umis using 96 reads
cdr3 = CASSLEGGTFQETQYF at 374, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 1322
start codons at 47, 146, 169, 189, 197, 239, 273
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.112 = ACATCAGTCGTTGACA-1

using 28 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.116 = ACATCAGTCTACTATC-1

using 296 reads

====================================================================================

graph has 234 edges initially, 8 edges after simplification

total ucounts = 31
nonsolo ucounts = 24[2, 3^3, 4, 8, 9^3, 12^2, 13^3, 14^4, 15, 16, 17^2, 20, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.118 = ACATCAGTCTCAACTT-1

using 21 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.119 = ACATCAGTCTCATTCA-1

using 61 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[2, 3, 4, 5^4, 6, 9, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.121 = ACATCAGTCTCTTGAT-1

using 38 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.130 = ACATCAGTCTTAGAGC-1

using 56 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.136 = ACATCAGTCTTGCCGT-1

using 64 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.137 = ACATCAGTCTTGGGTA-1

using 83 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.138 = ACATCAGTCTTTACGT-1

using 80000 reads

====================================================================================

graph has 49414 edges initially, 2049 edges after simplification

total ucounts = 5204
nonsolo ucounts = 4070[2^499, 3^381, 4^300, 5^278, 6^225, 7^200, 8^184, 9^160, 10^136, 11^124, 12^115, 13^122, 14^98, 15^92, 16^94, 17^91, 18^80, 19^75, 20^81, 21^67, 22^64, 23^53, 24^35, 25^53, 26^42, 27^38, 28^43, 29^27, 30^33, 31^22, 32^31, 33^17, 34^19, 35^17, 36^8, 37^20, 38^10, 39^13, 40^10, 41^7, 42^6, 43^4, 44^5, 45^6, 46^7, 47^4, 48^5, 49^6, 50^2, 51, 52^3, 54^2, 55^3, 56, 57^3, 58^2, 59, 60^2, 62^5, 64^2, 65, 67, 69, 70^2, 72^2, 73, 74^2, 76^2, 77^2, 79^3, 80, 85, 86, 88, 89, 96, 108, 109^2, 147, 495, 885, 1175, 2044, 2380, 2435, 3905, 7603, 8456]
surviving nonsolo ucounts = 8[495, 885, 1175, 2380, 2435, 3905, 7603, 8456]
ids = [3426, 1833, 633, 3693, 2256, 4506, 2680, 4646]

====================================================================================

REJECT CONTIGS

TIG 1[bases=321]
5-236 ==> 647-878 on segment before TRAJ33 exon 1 [len=878] (mis=0)
236-293 ==> 0-57 on |419|TRAJ33|J-REGION| [len=57] (mis=0)
293-321 ==> 0-28 on |393|TRAC|C-REGION| [len=422] (mis=1)
umis assigned: [2256, 2680, 3693]
of which 3 are surviving nonsolos
reads assigned: 7401
start codons at 16, 29, 56, 81, 91, 124, 142
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.141 = ACATGGTAGAATAGGG-1

using 54 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 4, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.143 = ACATGGTAGAGACTTA-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.144 = ACATGGTAGAGCCCAA-1

using 118 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 6, 11, 16, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.158 = ACATGGTAGCGAGAAA-1

using 99 reads

====================================================================================

graph has 96 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 4, 8, 9, 35, 38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.162 = ACATGGTAGCTTCGCG-1

using 822 reads

====================================================================================

graph has 410 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[822]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.163 = ACATGGTAGGAATTAC-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.164 = ACATGGTAGGACCACA-1

using 114 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2, 3^2, 4, 19, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.167 = ACATGGTAGGCTACGA-1

using 119 reads

====================================================================================

graph has 112 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 10[2, 5^2, 6, 8, 9, 14, 16, 23, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.168 = ACATGGTAGGGTCGAT-1

using 99 reads

====================================================================================

graph has 86 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 34, 55]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.170 = ACATGGTAGGTGACCA-1

using 996 reads

====================================================================================

graph has 624 edges initially, 26 edges after simplification

total ucounts = 40
nonsolo ucounts = 36[2^2, 4^2, 5, 6, 7, 8, 9^3, 10^3, 11, 17^2, 19^2, 21, 22, 27, 28, 33, 38, 43, 44^2, 46, 48^2, 53^2, 67, 82, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.171 = ACATGGTAGGTGATAT-1

using 27786 reads

====================================================================================

graph has 19815 edges initially, 910 edges after simplification

total ucounts = 2234
nonsolo ucounts = 1691[2^219, 3^172, 4^110, 5^102, 6^92, 7^72, 8^56, 9^55, 10^44, 11^52, 12^38, 13^34, 14^40, 15^43, 16^28, 17^39, 18^26, 19^33, 20^30, 21^19, 22^26, 23^19, 24^26, 25^16, 26^19, 27^20, 28^17, 29^16, 30^12, 31^10, 32^14, 33^8, 34^15, 35^9, 36^11, 37^13, 38^13, 39^17, 40^5, 41^4, 42^11, 43^6, 44^7, 45^6, 46^6, 47^4, 48^2, 49^4, 51^4, 52^2, 54^6, 57, 58^5, 59^2, 60^2, 61^3, 62, 63^2, 64, 65, 66, 68^3, 69^2, 70, 71, 72^2, 73, 74, 75, 76, 77, 79^2, 103^2, 106, 3099]
surviving nonsolo ucounts = 1[3099]
ids = [480]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.172 = ACATGGTAGGTGCTTT-1

using 69 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3^3, 4, 5^2, 7, 10, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.175 = ACATGGTAGTTAGGTA-1

using 45814 reads

====================================================================================

graph has 18937 edges initially, 522 edges after simplification

total ucounts = 1212
nonsolo ucounts = 927[2^107, 3^70, 4^74, 5^52, 6^48, 7^48, 8^23, 9^32, 10^20, 11^25, 12^20, 13^27, 14^21, 15^21, 16^19, 17^19, 18^11, 19^12, 20^12, 21^12, 22^13, 23^19, 24^16, 25^11, 26^6, 27^13, 28^8, 29^6, 30^12, 31^13, 32^5, 33^2, 34^11, 35^5, 36^5, 37^6, 38^3, 39^3, 41^4, 42^3, 43^12, 44^5, 45^4, 46^2, 47^4, 48^3, 49^4, 50, 51^2, 52^2, 53^2, 55^2, 56^2, 57^2, 58, 59^3, 61, 64, 65, 66, 67, 68^2, 69^2, 75, 79, 81, 82, 84, 87, 88, 95, 102, 103, 172, 234, 260, 393, 909, 1122, 1465, 1579, 1620, 1766, 1795, 1806, 1811, 1880, 1920, 2115, 2139, 2213, 2435, 3909]
surviving nonsolo ucounts = 19[234, 260, 393, 909, 1122, 1465, 1579, 1620, 1766, 1795, 1806, 1811, 1880, 1920, 2115, 2139, 2213, 2435, 3909]
ids = [85, 1208, 1091, 886, 860, 309, 590, 1055, 1107, 352, ...]

====================================================================================

UMI info for barcode ACATGGTAGTTAGGTA-1 contig 1 = ATGTTCACAG...
umi ACATGTCGAG = 213 reads: -387 +3 -1XX +1 -2XX +1 -2XX +3 invalidated
umi ACGTATATAG = 2135 reads: +400 validated
umi ATTGTCAGCT = 1881 reads: +400 validated
umi CAGTTGGCAC = 2205 reads: +400 validated
umi CGTAAGCCTT = 2120 reads: +400 validated
umi GTACCGCAGT = 1810 reads: +400 validated
umi TACAGTCCAG = 1327 reads: +400 validated
umi TAGCTGTCTG = 913 reads: +400 validated
umi TGCCTGTAAG = 1623 reads: +400 validated
umi TGTATAGCCT = 1805 reads: +400 validated
umi TGTCGAGTTT = 364 reads: -374X +1 -1XX +1 -3XX +1 -3XX +2 -9XX +2 -1XX +2 invalidated
umi TTACACTCGA = 1766 reads: +400 validated
umi TTGTTTTCCA = 1937 reads: +400 validated
umi TTTTTATCCC = 262 reads: +400 validated

UMI info for barcode ACATGGTAGTTAGGTA-1 contig 2 = GGGACACTAA...
umi CAGCCTATTT = 1434 reads: +403 validated
umi CATTGGGATT = 1802 reads: +403 validated
umi CCTCAATTTG = 3895 reads: +403 validated

UMI info for barcode ACATGGTAGTTAGGTA-1 contig 3 = GGGGACTCTT...
umi ATCCATCTGG = 1 reads: -375X +3 -8X +1 -1X invalidated
umi CTTCCTTGCC = 1594 reads: +388 validated
umi GGCCTTGCCC = 2589 reads: +388 validated
umi TTAGGATCGT = 1 reads: -375X +1 -2X +1 -2X +1 -4X +1 -1X invalidated

GOOD CONTIGS

TIG 1[bases=504]
0-52 ==> 7-59 on |569|TRBV12-4|5'UTR| [len=59] (mis=0)
52-399 ==> 0-347 on |570|TRBV12-4|L-REGION+V-REGION| [len=347] (mis=3)
404-452 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
452-504 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 11 umis using 1308 reads
cdr3 = CASSSDRTNTEAFF at 382, score = 8 + 8
umis assigned: [85, 121, 247, 322, 499, 776, 860, 886, 1055, 1083] and 4 others
of which 14 are surviving nonsolos
reads assigned: 19841
start codons at 0, 52, 110, 151, 174, 223, 226, 275, 286, 313, 320
confident = true

TIG 2[bases=457]
12-350 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
346-415 ==> 0-69 on |439|TRAJ52|J-REGION| [len=69] (mis=3)
415-457 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 3 umis using 466 reads
cdr3 = CALSRAGGTSYGKLTF at 336, score = 9 + 9
umis assigned: [309, 352, 440]
of which 3 are surviving nonsolos
reads assigned: 7013
start codons at 12, 87, 175, 229, 367
confident = true

TIG 3[bases=527]
0-97 ==> 0-97 on |492|TRAV27|5'UTR| [len=97] (mis=3)
97-425 ==> 0-328 on |493|TRAV27|L-REGION+V-REGION| [len=328] (mis=5)
426-485 ==> 0-59 on |422|TRAJ36|J-REGION| [len=59] (mis=2)
485-527 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 223 reads
cdr3 = CALGETGANNLFF at 415, score = 10 + 9
umis assigned: [211, 590, 738, 1117]
of which 2 are surviving nonsolos
reads assigned: 3980
start codons at 70, 79, 97, 141, 249, 350
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.176 = ACATGGTAGTTCCACA-1

using 52 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.177 = ACATGGTAGTTGAGTA-1

using 12315 reads

====================================================================================

graph has 4665 edges initially, 98 edges after simplification

total ucounts = 402
nonsolo ucounts = 275[2^58, 3^36, 4^24, 5^11, 6^13, 7^11, 8^14, 9^10, 10^6, 11^5, 12^5, 13^5, 14^8, 15^4, 16^4, 17^2, 18^5, 19^3, 20^2, 22^3, 23^6, 24, 25, 26^3, 27, 28^2, 29, 30, 31^3, 32^4, 33, 34^2, 35^2, 38^2, 40, 41^3, 48, 51, 55, 57, 58, 61, 70, 92, 789, 1080, 3610, 3684]
surviving nonsolo ucounts = 4[789, 1080, 3610, 3684]
ids = [139, 268, 4, 133]

====================================================================================

UMI info for barcode ACATGGTAGTTGAGTA-1 contig 1 = TGGGGAATGT...
umi AAAGTCCTGT = 3625 reads: +388 validated
umi GGGTTAGCCA = 982 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=451]
21-348 ==> 0-327 on |491|TRAV26-2|L-REGION+V-REGION| [len=327] (mis=0)
346-409 ==> 0-63 on |398|TRAJ13|J-REGION| [len=63] (mis=5)
409-451 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 303 reads
cdr3 = CILRDITGGYQKVTF at 333, score = 8 + 8
umis assigned: [4, 268]
of which 2 are surviving nonsolos
reads assigned: 4527
start codons at 6, 21, 66, 73, 99, 217, 232, 246, 316
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.178 = ACATGGTAGTTGTAGA-1

using 45 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.179 = ACATGGTAGTTTAGGA-1

using 98 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[12, 86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.180 = ACATGGTCAAAGGCGT-1

using 86 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.184 = ACATGGTCAAGCTGTT-1

using 123 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[123]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.186 = ACATGGTCAATCCAAC-1

using 134 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 6, 55, 69]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.187 = ACATGGTCAATCTACG-1

using 50 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.190 = ACATGGTCACAGACAG-1

using 31 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.194 = ACATGGTCACCATCCT-1

using 45 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.199 = ACATGGTCACTGTGTA-1

using 21 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.200 = ACATGGTCACTGTTAG-1

using 49 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.202 = ACATGGTCAGATCCAT-1

using 69 reads

====================================================================================

graph has 52 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 7^2, 14, 15, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.208 = ACATGGTCAGCGAACA-1

using 51 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[51]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.209 = ACATGGTCAGCTCGCA-1

using 56 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.210 = ACATGGTCAGCTGCAC-1

using 31 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.211 = ACATGGTCAGCTGTAT-1

using 44 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.212 = ACATGGTCAGGAACGT-1

using 9697 reads

====================================================================================

graph has 6931 edges initially, 290 edges after simplification

total ucounts = 985
nonsolo ucounts = 732[2^97, 3^88, 4^58, 5^55, 6^36, 7^37, 8^35, 9^26, 10^20, 11^19, 12^16, 13^16, 14^16, 15^16, 16^14, 17^10, 18^7, 19^15, 20^16, 21^8, 22^7, 23^8, 24^7, 25^8, 26^9, 27^6, 28, 29^6, 30^2, 31^4, 32^3, 33^4, 34^2, 35^3, 36^2, 37^2, 38^4, 39^4, 40, 41^2, 42^2, 43^4, 44^2, 45, 46^2, 47, 50^2, 51^2, 53^2, 54^2, 55, 56, 57^2, 58^2, 59, 62^3, 64, 65, 67, 68, 71, 72^2, 76, 81, 84, 91, 117]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.215 = ACATGGTCAGGGTACA-1

using 76 reads

====================================================================================

graph has 42 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[5, 6, 7, 10, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.216 = ACATGGTCAGTAACGG-1

using 90 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 7, 27, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.220 = ACATGGTCATACAGCT-1

using 57 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 10[2^3, 3^2, 4^3, 5, 27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.223 = ACATGGTCATATGGTC-1

using 113 reads

====================================================================================

graph has 88 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[4, 5^2, 8, 16, 35, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.224 = ACATGGTCATCACAAC-1

using 63 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 7^2, 9, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.227 = ACATGGTCATCGATTG-1

using 85 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[9, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.229 = ACATGGTCATGACATC-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.236 = ACATGGTGTAAAGTCA-1

using 110 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3^2, 4^2, 5, 15, 31, 44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.237 = ACATGGTGTAAGAGAG-1

using 14 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.238 = ACATGGTGTAAGGGAA-1

using 57 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[3, 5, 18, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.240 = ACATGGTGTACCTACA-1

using 145 reads

====================================================================================

graph has 169 edges initially, 6 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2^2, 3^3, 4, 5^2, 6^2, 11, 23, 30, 39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.247 = ACATGGTGTCAGAAGC-1

using 43 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.248 = ACATGGTGTCAGAATA-1

using 549 reads

====================================================================================

graph has 358 edges initially, 24 edges after simplification

total ucounts = 29
nonsolo ucounts = 26[2^2, 3^4, 4, 6, 7^3, 8^3, 10, 11, 12, 32, 33, 35, 36, 38, 42, 53, 80, 93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.252 = ACATGGTGTCGAACAG-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.256 = ACATGGTGTCTAGCCG-1

using 58 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.258 = ACATGGTGTCTCTTTA-1

using 45 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[45]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.263 = ACATGGTGTGCAACTT-1

using 207 reads

====================================================================================

graph has 214 edges initially, 6 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^4, 4, 11, 12, 23, 34, 44, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.264 = ACATGGTGTGGCAAAC-1

using 49 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.265 = ACATGGTGTGGTACAG-1

using 92 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.266 = ACATGGTGTGGTCTCG-1

using 64 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[64]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.267 = ACATGGTGTGTAATGA-1

using 49 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.269 = ACATGGTGTGTGCCTG-1

using 58 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[58]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.271 = ACATGGTGTGTTTGTG-1

using 37 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.272 = ACATGGTGTTACCAGT-1

using 67 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.275 = ACATGGTGTTCCACAA-1

using 17 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.276 = ACATGGTGTTCCGGCA-1

using 26 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.278 = ACATGGTGTTCTGAAC-1

using 63 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.287 = ACATGGTTCAACCATG-1

using 202 reads

====================================================================================

graph has 206 edges initially, 6 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[3, 6, 9^2, 15, 29, 32, 37, 61]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.291 = ACATGGTTCACTATTC-1

using 44 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.293 = ACATGGTTCACTTCAT-1

using 151 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 17
nonsolo ucounts = 14[2, 4^3, 5, 6^2, 7^2, 8, 10, 15, 30, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.295 = ACATGGTTCAGCACAT-1

using 30 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 4, 8, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.297 = ACATGGTTCAGGTAAA-1

using 46 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.302 = ACATGGTTCATCTGTT-1

using 47 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.304 = ACATGGTTCATGGTCA-1

using 56 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[12, 16, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.306 = ACATGGTTCATTGCGA-1

using 71 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[4, 6, 7, 10, 11, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.308 = ACATGGTTCCACGAAT-1

using 89 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.311 = ACATGGTTCCCTAACC-1

using 49 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.319 = ACATGGTTCCTGTACC-1

using 1786 reads

====================================================================================

graph has 800 edges initially, 36 edges after simplification

total ucounts = 74
nonsolo ucounts = 63[2^5, 4^2, 5^3, 7^3, 8^3, 9^3, 10^2, 11^5, 13^2, 15^2, 17, 20^2, 21, 22^2, 24, 25, 26, 27, 28, 29, 33, 34, 36, 37, 40, 46, 47, 48, 52, 53^2, 57^3, 59, 71, 80, 82, 87, 109, 120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.320 = ACATGGTTCCTTTCTC-1

using 152 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2, 7, 22, 26, 90]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.322 = ACATGGTTCGCCTGAG-1

using 21 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.325 = ACATGGTTCGCTAGCG-1

using 18085 reads

====================================================================================

graph has 8937 edges initially, 228 edges after simplification

total ucounts = 901
nonsolo ucounts = 684[2^110, 3^81, 4^58, 5^51, 6^29, 7^33, 8^25, 9^34, 10^25, 11^20, 12^14, 13^6, 14^14, 15^12, 16^12, 17^12, 18^12, 19^10, 20^3, 21^6, 22^6, 23^7, 24^3, 25^4, 26^2, 27^6, 28^3, 29^5, 30^8, 31^3, 32^6, 34^2, 35^5, 36^5, 37^3, 38^2, 39^3, 40^5, 41, 42, 43^2, 44, 45, 46^2, 47^2, 48^2, 49^3, 50, 54, 55, 56^2, 57, 58^2, 60^2, 62, 64, 65^2, 67, 75^2, 83^2, 84, 86, 98, 1801, 7608]
surviving nonsolo ucounts = 2[1801, 7608]
ids = [895, 677]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.328 = ACATGGTTCGTCGTTC-1

using 54 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.332 = ACATGGTTCTAGAGTC-1

using 86 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 4, 6^2, 12, 15^2, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.335 = ACATGGTTCTCCCTGA-1

using 60 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.338 = ACATGGTTCTGCTTGC-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.340 = ACATGGTTCTGGTGTA-1

using 26 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.341 = ACATGGTTCTTAGCCC-1

using 76 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.342 = ACATGGTTCTTATCTG-1

using 53 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2^2, 3, 18, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.345 = ACATGGTTCTTGCAAG-1

using 62 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[62]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.346 = ACATGGTTCTTGTACT-1

using 35111 reads

====================================================================================

graph has 18436 edges initially, 690 edges after simplification

total ucounts = 1922
nonsolo ucounts = 1497[2^184, 3^154, 4^98, 5^93, 6^82, 7^61, 8^46, 9^53, 10^37, 11^52, 12^37, 13^52, 14^43, 15^42, 16^29, 17^28, 18^29, 19^30, 20^25, 21^24, 22^28, 23^29, 24^18, 25^23, 26^20, 27^18, 28^8, 29^14, 30^12, 31^5, 32^5, 33^7, 34^14, 35^6, 36^11, 37^9, 38^3, 39^4, 40^11, 41^3, 42^4, 43^4, 44^6, 45^4, 46^3, 47^2, 48^3, 49^2, 52, 54, 55, 56^2, 57, 59, 63, 65, 67^2, 68^2, 69, 70, 71^2, 76, 86, 105, 6889, 8343]
surviving nonsolo ucounts = 3[34, 6889, 8343]
ids = [1737, 1741, 373]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.351 = ACCAGTAAGAATTGTG-1

using 40 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.357 = ACCAGTAAGAGTAAGG-1

using 101 reads

====================================================================================

graph has 74 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[21, 26, 53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.366 = ACCAGTAAGGCCCTCA-1

using 147 reads

====================================================================================

graph has 124 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[3, 6, 10, 12, 23, 33, 56]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.367 = ACCAGTAAGGCGACAT-1

using 55 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[53]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.369 = ACCAGTAAGGGATACC-1

using 48 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 18, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.372 = ACCAGTAAGGTGCTTT-1

using 94 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.373 = ACCAGTAAGTACGCGA-1

using 1489 reads

====================================================================================

graph has 516 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[1485]
surviving nonsolo ucounts = 1[1485]
ids = [3]

====================================================================================

UMI info for barcode ACCAGTAAGTACGCGA-1 contig 1 = TGGGACAGTG...
umi CCATTAGGCA = 1486 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=524]
85-425 ==> 0-340 on |514|TRAV5|L-REGION+V-REGION| [len=340] (mis=0)
422-482 ==> 0-60 on |400|TRAJ16|J-REGION| [len=60] (mis=6)
482-524 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CAESRTDGQKLLF at 412, score = 7 + 6
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 1464
start codons at 85, 139, 155, 304, 310, 431, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.374 = ACCAGTAAGTCCTCCT-1

using 88 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.381 = ACCAGTACAACACGCC-1

using 103 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[103]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.382 = ACCAGTACAAGCGATG-1

using 137 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 130]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.383 = ACCAGTACAAGCTGAG-1

using 32 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 5^2, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.386 = ACCAGTACAATGCCAT-1

using 68 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 60]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.390 = ACCAGTACACACAGAG-1

using 111 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[22, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.393 = ACCAGTACACAGATTC-1

using 31 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.396 = ACCAGTACACCAGTTA-1

using 36 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[3, 4, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.399 = ACCAGTACACGCCAGT-1

using 757 reads

====================================================================================

graph has 538 edges initially, 20 edges after simplification

total ucounts = 35
nonsolo ucounts = 23[2^2, 4, 5^2, 6, 11, 16, 21, 29, 31^2, 33, 34, 39, 47, 49, 50, 56, 58, 62, 67, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.400 = ACCAGTACACGGCCAT-1

using 28 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.402 = ACCAGTACACTCGACG-1

using 55 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.407 = ACCAGTACAGCATACT-1

using 74 reads

====================================================================================

graph has 66 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 12, 21, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.408 = ACCAGTACAGCTGCAC-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.413 = ACCAGTACATCGTCGG-1

using 67 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.416 = ACCAGTACATGCGCAC-1

using 32 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2^3, 4, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.419 = ACCAGTACATTCCTCG-1

using 101 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 11[2, 3, 4, 5, 6, 10^2, 11, 14, 16, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.421 = ACCAGTACATTTCACT-1

using 38 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.422 = ACCAGTAGTAAATACG-1

using 63 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[63]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.427 = ACCAGTAGTACGACCC-1

using 92 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.437 = ACCAGTAGTCCCGACA-1

using 88 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.439 = ACCAGTAGTCCTCTTG-1

using 72 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.443 = ACCAGTAGTCGTCTTC-1

using 48 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.448 = ACCAGTAGTGGCAAAC-1

using 77 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.450 = ACCAGTAGTGTCTGAT-1

using 1200 reads

====================================================================================

graph has 422 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 6, 1189]
surviving nonsolo ucounts = 1[1189]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.453 = ACCAGTAGTTAGGGTG-1

using 38 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.459 = ACCAGTAGTTCCTCCA-1

using 90 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 80]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.460 = ACCAGTAGTTCTGGTA-1

using 77 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[77]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.461 = ACCAGTAGTTGAGGTG-1

using 26 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.473 = ACCAGTATCAGTTGAC-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.476 = ACCAGTATCATCGGAT-1

using 64896 reads

====================================================================================

graph has 17093 edges initially, 333 edges after simplification

total ucounts = 836
nonsolo ucounts = 633[2^88, 3^65, 4^32, 5^27, 6^28, 7^35, 8^25, 9^13, 10^22, 11^17, 12^15, 13^15, 14^14, 15^20, 16^13, 17^10, 18^13, 19^13, 20^9, 21^15, 22^6, 23^6, 24^8, 25^10, 26^10, 27^3, 28^6, 29^6, 30^2, 31^5, 32^3, 33^4, 35^4, 36, 37^2, 38, 39^2, 40^2, 41^3, 42, 43^3, 44^2, 45^2, 47, 48^3, 50, 51^3, 52, 53^2, 54^2, 55^3, 58, 61, 62, 64^2, 72, 73^2, 74^2, 78^2, 79, 85, 89, 92, 99, 112, 309, 551, 634, 740, 743, 862, 923, 1253, 1659, 1684, 1836, 1868, 5076, 5784, 6645, 7970, 8103, 9089]
surviving nonsolo ucounts = 18[309, 551, 634, 740, 743, 862, 923, 1253, 1659, 1684, 1836, 1868, 5076, 5784, 6645, 7970, 8103, 9089]
ids = [152, 809, 496, 449, 487, 310, 436, 815, 97, 544, ...]

====================================================================================

UMI info for barcode ACCAGTATCATCGGAT-1 contig 1 = GGGGAGTTTC...
umi CTGCTGAGTA = 929 reads: +364 -10 +17 non-validated
umi GAGATGTGCA = 771 reads: +391 validated
umi GATGCTTTAG = 562 reads: -356 +7 -1XX +1 -1XX +5 -3XX +1 -1XX +4 -1XX +2 -3XX +3 -2XX invalidated
umi TTGACTATCT = 545 reads: +368 -23 non-validated
umi TTGGGCCTAG = 1118 reads: +391 validated

UMI info for barcode ACCAGTATCATCGGAT-1 contig 2 = ATGCTCACAG...
umi AACCACGTTC = 1868 reads: +400 validated
umi ACGGAAGTCA = 1674 reads: +400 validated
umi CCCCGACACC = 796 reads: -363 +4 -1XX +9 -1XX +2 -1XX +2 -2XX +7 -1XX +2 -3XX +2 invalidated
umi GGCATCCTTA = 1690 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=671]
238-573 ==> 0-335 on |522|TRAV8-3|L-REGION+V-REGION| [len=341] (mis=0)
575-629 ==> 0-54 on |430|TRAJ43|J-REGION| [len=54] (mis=2)
629-671 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 59 reads
cdr3 = CAVINNNDMRF at 565, score = 7 + 8
umis assigned: [436, 487, 496, 809, 815]
of which 5 are surviving nonsolos
reads assigned: 3847
start codons at 238, 360, 374, 401, 548, 584, 589
confident = true

TIG 2[bases=504]
0-52 ==> 10-62 on |565|TRBV12-3|5'UTR| [len=62] (mis=0)
52-399 ==> 0-347 on |566|TRBV12-3|L-REGION+V-REGION| [len=347] (mis=2)
405-452 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=4)
452-504 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 3 umis using 310 reads
cdr3 = CASSLQGGGYEQYF at 382, score = 8 + 8
umis assigned: [21, 97, 310, 544]
of which 4 are surviving nonsolos
reads assigned: 5925
start codons at 0, 52, 110, 137, 151, 174, 223, 226, 275, 286, 313, 320
confident = true

REJECT CONTIGS

TIG 1[bases=477]
5-365 ==> 5640-6000 on segment before TRBD1 exon 1 [len=6000] (mis=0)
375-425 ==> 0-50 on |540|TRBJ1-5|J-REGION| [len=50] (mis=1)
425-477 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
umis assigned: [14, 37, 199, 224, 279, 335, 449, 457, 502, 747]
of which 8 are surviving nonsolos
reads assigned: 44521
start codons at 38, 66, 242, 252, 326, 401
confident = false
did not find CDR3
now this is a cell
paired!

CCCTCTGTGCATTGGAGTGATGCTGCTGAGTACTTCTGTGCTGTTATAAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACCAA <==> TCAGAACCCAGGGACTCAGCTGTGTACTTCTGTGCCAGCAGTTTACAGGGGGGAGGGTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.481 = ACCAGTATCCAATGGT-1

using 154 reads

====================================================================================

graph has 60 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[35, 37, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.484 = ACCAGTATCCCAAGAT-1

using 36 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.486 = ACCAGTATCCGAAGAG-1

using 29 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.487 = ACCAGTATCCGGGTGT-1

using 34 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 4[2^2, 8, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.488 = ACCAGTATCCTCGCAT-1

using 27 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.491 = ACCAGTATCGTTACGA-1

using 121 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2, 3, 5, 8, 10, 11, 81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.492 = ACCAGTATCGTTGACA-1

using 129 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2, 4^3, 18, 23, 35, 36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.500 = ACCAGTATCTGTCTCG-1

using 142 reads

====================================================================================

graph has 154 edges initially, 8 edges after simplification

total ucounts = 17
nonsolo ucounts = 13[2^3, 3, 7, 8, 9, 10, 11, 19, 20, 21, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.501 = ACCAGTATCTTATCTG-1

using 38 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[38]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.502 = ACCAGTATCTTCCTTC-1

using 36 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[36]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.504 = ACCAGTATCTTTAGTC-1

using 65 reads

====================================================================================

graph has 62 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[28, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.508 = ACCCACTAGAATTCCC-1

using 65 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 9[2^3, 3, 4, 6, 7, 14, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.511 = ACCCACTAGAGACTAT-1

using 56 reads

====================================================================================

graph has 48 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 6, 16, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.512 = ACCCACTAGAGGGCTT-1

using 831 reads

====================================================================================

graph has 582 edges initially, 40 edges after simplification

total ucounts = 55
nonsolo ucounts = 45[2^6, 3^6, 4^4, 5^2, 6, 7, 8^2, 9, 11^2, 12, 13, 14^2, 16, 17^3, 20, 21, 26, 32, 39, 43, 51, 55, 64, 69, 78, 87]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.517 = ACCCACTAGATCCTGT-1

using 18 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.519 = ACCCACTAGCCAGTTT-1

using 35 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.522 = ACCCACTAGCGCTTAT-1

using 49 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[49]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.524 = ACCCACTAGCGTCAAG-1

using 49 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[4, 6, 7, 11, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.525 = ACCCACTAGCGTTTAC-1

using 66 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[6^2, 12, 19, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.529 = ACCCACTAGGAGCGAG-1

using 33 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.530 = ACCCACTAGGGTGTGT-1

using 23 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.531 = ACCCACTAGGTCGGAT-1

using 42 reads

====================================================================================

graph has 91 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[7, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.532 = ACCCACTAGGTGCTTT-1

using 92 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[16, 76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.533 = ACCCACTAGTAGCGGT-1

using 78 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[78]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.534 = ACCCACTAGTAGGCCA-1

using 1743 reads

====================================================================================

graph has 470 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 13, 15, 1706]
surviving nonsolo ucounts = 1[1706]
ids = [9]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.535 = ACCCACTAGTATCTCG-1

using 25994 reads

====================================================================================

graph has 10867 edges initially, 296 edges after simplification

total ucounts = 844
nonsolo ucounts = 661[2^91, 3^56, 4^46, 5^48, 6^28, 7^34, 8^37, 9^31, 10^18, 11^19, 12^18, 13^20, 14^18, 15^18, 16^10, 17^13, 18^9, 19^12, 20^8, 21^12, 22^8, 23^4, 24^7, 25^9, 26^6, 27^6, 28^2, 29^2, 30^4, 31^7, 32^2, 33^3, 34^2, 35^2, 36^4, 37^4, 38^4, 39, 41^2, 42^2, 44, 45, 46, 47, 48^2, 49, 50^3, 51, 54, 55^2, 61, 67, 70, 77, 83, 85, 91, 154, 365, 664, 1134, 1203, 1295, 1304, 1387, 1789, 1925, 2030, 2047, 2583]
surviving nonsolo ucounts = 12[365, 664, 1134, 1203, 1295, 1304, 1387, 1789, 1925, 2030, 2047, 2583]
ids = [421, 48, 467, 147, 778, 618, 763, 653, 61, 490, ...]

====================================================================================

UMI info for barcode ACCCACTAGTATCTCG-1 contig 1 = GCCAGGTTCA...
umi AATTGAGGCT = 670 reads: +406 validated
umi TGCGGACTCA = 1382 reads: +406 validated

UMI info for barcode ACCCACTAGTATCTCG-1 contig 2 = GTCATCCCTC...
umi ACAGTTGCTG = 1928 reads: +385 validated
umi ACATTACTTG = 2569 reads: +385 validated
umi CTCCCTTCTG = 308 reads: -373 +4 -1XX +2 -3XX +2 invalidated
umi GCACCCGGTT = 2037 reads: +385 validated
umi TACCCCTCTC = 1297 reads: +385 validated
umi TATTTCATGC = 1792 reads: +385 validated
umi TCCATGGGTG = 1 reads: -383X +1 -1X invalidated
umi TGTGTCACCG = 1278 reads: +385 validated
umi TTCACTGCTC = 2056 reads: +385 validated

UMI info for barcode ACCCACTAGTATCTCG-1 contig 3 = TGGGGAAACA...
umi AGGCGCGCTT = 1200 reads: +463 validated
umi GAAGATAGGT = 1136 reads: +463 validated

GOOD CONTIGS

TIG 1[bases=523]
0-75 ==> 0-75 on |467|TRAV14/DV4|5'UTR| [len=75] (mis=3)
75-425 ==> 0-350 on |468|TRAV14/DV4|L-REGION+V-REGION| [len=350] (mis=3)
429-481 ==> 8-60 on |414|TRAJ29|J-REGION| [len=60] (mis=0)
481-523 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 82 reads
cdr3 = CAMREGMGNTPLVF at 408, score = 7 + 9
umis assigned: [48, 763]
of which 2 are surviving nonsolos
reads assigned: 2023
start codons at 75, 165, 208, 229, 267, 295, 307, 399, 414, 426
confident = true

TIG 2[bases=515]
0-78 ==> 0-78 on |595|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |596|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
415-463 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
463-515 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 7 umis using 751 reads
cdr3 = CSGGDKDTEAFF at 399, score = 7 + 8
umis assigned: [61, 71, 421, 490, 618, 653, 685, 778, 798]
of which 8 are surviving nonsolos
reads assigned: 13078
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

TIG 3[bases=517]
12-409 ==> 0-397 on |516|TRAV6|L-REGION+V-REGION| [len=397] (mis=3)
414-475 ==> 0-61 on |450|TRAJ9|J-REGION| [len=61] (mis=1)
475-517 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 97 reads
cdr3 = CALDEVRNTGGFKTIF at 396, score = 8 + 8
umis assigned: [147, 467]
of which 2 are surviving nonsolos
reads assigned: 2305
start codons at 12, 69, 286
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.536 = ACCCACTAGTATTGGA-1

using 88 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 7, 13, 25, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.537 = ACCCACTAGTCCATAC-1

using 76 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.540 = ACCCACTAGTGACATA-1

using 105 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 3^2, 4^3, 6, 75]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.545 = ACCCACTCAAATTGCC-1

using 41 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.548 = ACCCACTCAAGCCGCT-1

using 72 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[72]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.549 = ACCCACTCAAGGTTCT-1

using 296 reads

====================================================================================

graph has 140 edges initially, 10 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[3, 9, 13, 19, 20, 21, 37, 72, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.551 = ACCCACTCAATAAGCA-1

using 65 reads

====================================================================================

graph has 70 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 9, 24, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.559 = ACCCACTCACATTAGC-1

using 34 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.560 = ACCCACTCACATTTCT-1

using 27 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.561 = ACCCACTCACCACCAG-1

using 102 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 8, 89]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.563 = ACCCACTCACCAGTTA-1

using 30617 reads

====================================================================================

graph has 14647 edges initially, 448 edges after simplification

total ucounts = 1728
nonsolo ucounts = 1321[2^200, 3^169, 4^115, 5^94, 6^85, 7^67, 8^60, 9^58, 10^34, 11^39, 12^43, 13^43, 14^29, 15^29, 16^19, 17^19, 18^21, 19^23, 20^15, 21^14, 22^11, 23^13, 24^8, 25^14, 26^11, 27^6, 28^6, 29^9, 30^7, 31^3, 32^6, 33^2, 34^2, 35^2, 36^3, 37^2, 38^3, 39^2, 40, 41, 42^2, 44^3, 45^2, 46, 47^4, 51, 54^3, 57^2, 60, 64, 65, 67, 75, 81, 82, 83^2, 107, 150, 167, 883, 7600, 8123]
surviving nonsolo ucounts = 2[7600, 8123]
ids = [878, 36]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.568 = ACCCACTCACGTTGGC-1

using 74 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[74]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.570 = ACCCACTCACTTAACG-1

using 115 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[115]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.572 = ACCCACTCAGCCACCA-1

using 127 reads

====================================================================================

graph has 64 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2, 3, 5, 13, 18, 85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.573 = ACCCACTCAGCCAGAA-1

using 97 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.574 = ACCCACTCAGCTCGCA-1

using 85 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.579 = ACCCACTCAGGATTGG-1

using 94 reads

====================================================================================

graph has 72 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 5, 8, 9, 11, 13, 43]
surviving nonsolo ucounts = 1[43]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.580 = ACCCACTCAGGCTCAC-1

using 85 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[15, 70]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.583 = ACCCACTCAGTCGATT-1

using 39 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.585 = ACCCACTCATAACCTG-1

using 93 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.588 = ACCCACTCATCACCCT-1

using 1966 reads

====================================================================================

graph has 570 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^2, 4, 6, 43, 1907]
surviving nonsolo ucounts = 1[1907]
ids = [2]

====================================================================================

UMI info for barcode ACCCACTCATCACCCT-1 contig 1 = AACTCTCCCC...
umi AGCGATTACC = 1911 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=483]
0-40 ==> 38-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
40-375 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=1)
383-431 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=5)
431-483 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 1 umis using 120 reads
cdr3 = CSARAPEEGAEAFF at 361, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 1878
start codons at 40, 178, 214, 220, 233, 293
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.591 = ACCCACTCATCCGTGG-1

using 68 reads

====================================================================================

graph has 68 edges initially, 6 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[2, 3, 4, 5, 6, 8, 15, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.592 = ACCCACTCATGACATC-1

using 46 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.596 = ACCCACTCATTCCTCG-1

using 76 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[76]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.599 = ACCCACTGTAAATGAC-1

using 43 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 4, 15, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.600 = ACCCACTGTAAGAGGA-1

using 51 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 6[6^3, 9, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.601 = ACCCACTGTACAGTTC-1

using 27 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.603 = ACCCACTGTACCGTAT-1

using 137 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2, 5, 6, 8, 9, 10, 23, 67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.607 = ACCCACTGTATATGAG-1

using 18605 reads

====================================================================================

graph has 9274 edges initially, 270 edges after simplification

total ucounts = 886
nonsolo ucounts = 669[2^107, 3^58, 4^46, 5^46, 6^32, 7^35, 8^36, 9^29, 10^17, 11^27, 12^14, 13^8, 14^10, 15^14, 16^11, 17^14, 18^6, 19^7, 20^10, 21^13, 22^9, 23^11, 24^3, 25^8, 26^9, 27^3, 28^4, 29^5, 30^2, 31^8, 32^5, 33, 34^3, 35^2, 36^5, 37^4, 38^6, 39, 40^7, 43, 44^2, 45^3, 48, 49, 51^2, 52, 53^2, 54, 58, 60, 61^2, 62, 64, 69, 75, 77, 78, 90, 372, 568, 595, 826, 1610, 1615, 1653, 2869]
surviving nonsolo ucounts = 8[372, 568, 595, 826, 1610, 1615, 1653, 2869]
ids = [450, 316, 510, 297, 432, 159, 327, 62]

====================================================================================

UMI info for barcode ACCCACTGTATATGAG-1 contig 1 = GTCATCCCTC...
umi CGAAGTTCCT = 573 reads: +388 validated
umi CGCAGAATTA = 1666 reads: +388 validated
umi GAATGAGGGT = 1624 reads: +388 validated
umi GATCTCTCCT = 372 reads: +388 validated
umi GGCGCTCGCT = 598 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=518]
0-78 ==> 0-78 on |597|TRBV20-1|5'UTR| [len=78] (mis=0)
78-413 ==> 0-335 on |598|TRBV20-1|L-REGION+V-REGION| [len=335] (mis=3)
418-466 ==> 0-48 on |536|TRBJ1-1|J-REGION| [len=48] (mis=2)
466-518 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 4 umis using 228 reads
cdr3 = CSARRQQNTEAFF at 399, score = 7 + 8
umis assigned: [316, 327, 432, 450, 510]
of which 5 are surviving nonsolos
reads assigned: 4744
start codons at 21, 78, 216, 252, 258, 271, 331
confident = true

REJECT CONTIGS

TIG 1[bases=604]
0-56 ==> 0-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=1)
56-394 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=4)
503-562 ==> 0-59 on |412|TRAJ27|J-REGION| [len=59] (mis=0)
562-604 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
cdr3 = CALSTPLITGANSKLTF at 380, score = 9 + 8
umis assigned: [159, 297]
of which 2 are surviving nonsolos
reads assigned: 2400
start codons at 15, 56, 131, 219, 273, 511, 535
confident = false
frameshifted full length stopped transcript of length 604
VJ delta = -78
delta too large
not full
not full

TIG 2[bases=335]
5-141 ==> 595-731 on segment before TRAJ49 exon 1 [len=835] (mis=0)
120-237 ==> 718-835 on segment before TRAJ49 exon 1 [len=835] (mis=0)
237-293 ==> 0-56 on |436|TRAJ49|J-REGION| [len=56] (mis=0)
293-335 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
umis assigned: [62]
of which 1 are surviving nonsolos
reads assigned: 2836
start codons at 43, 119
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.608 = ACCCACTGTATATGGA-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.611 = ACCCACTGTCAGAATA-1

using 25 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 10^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.613 = ACCCACTGTCATACTG-1

using 20656 reads

====================================================================================

graph has 10895 edges initially, 362 edges after simplification

total ucounts = 1221
nonsolo ucounts = 900[2^128, 3^99, 4^66, 5^58, 6^50, 7^45, 8^43, 9^31, 10^28, 11^33, 12^32, 13^25, 14^16, 15^20, 16^26, 17^11, 18^12, 19^21, 20^19, 21^9, 22^16, 23^7, 24^11, 25^7, 26^5, 27^6, 28^6, 29^3, 30^6, 31^5, 32^5, 33^3, 34, 35^2, 36, 37, 38^2, 39, 40^4, 41, 42^4, 47, 50, 51^2, 52, 53, 54^2, 55, 62, 63, 66, 69, 71, 78^2, 82, 84, 89, 145, 318, 403, 406, 713, 873, 960, 1125, 1129, 1184, 1212, 1894]
surviving nonsolo ucounts = 12[24, 318, 403, 406, 713, 873, 960, 1125, 1129, 1184, 1212, 1894]
ids = [1070, 963, 454, 1085, 413, 717, 392, 1208, 350, 725, ...]

====================================================================================

UMI info for barcode ACCCACTGTCATACTG-1 contig 1 = TTCCTCTGCT...
umi CCCCTGGTGG = 1117 reads: +343 -43 +8 non-validated
umi CCTGACCGAG = 917 reads: +394 validated
umi CGACTTAGAT = 600 reads: -373 +1 -1 +2 -2XX +2 -1XX +1 -1XX +5 -2XX +3 invalidated
umi CGGGCAATTA = 1201 reads: +394 validated
umi CGTACCGGTT = 386 reads: +394 validated
umi GGAATGCCAT = 867 reads: +378 -10 +6 non-validated
umi GGATTAGGGC = 1175 reads: +372 -4 +18 non-validated
umi TCATGTAGGC = 309 reads: +362 -32 non-validated
umi TGTAGTCGAA = 396 reads: +394 validated
umi TTTGAGGGAT = 1110 reads: +394 validated

UMI info for barcode ACCCACTGTCATACTG-1 contig 2 = ATTTGGGTAA...
umi TCACGTTACG = 1903 reads: +391 validated
umi TGCTTACTTC = 13 reads: -386X +2 -1XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=558]
0-112 ==> 0-112 on |618|TRBV30|5'UTR| [len=112] (mis=0)
112-447 ==> 0-335 on |619|TRBV30|L-REGION+V-REGION| [len=335] (mis=1)
447-459 ==> 0-12 on |535|TRBD1|D-REGION| [len=12] (mis=2)
458-506 ==> 0-48 on |537|TRBJ1-2|J-REGION| [len=48] (mis=2)
506-558 ==> 0-52 on |531|TRBC1|C-REGION| [len=530] (mis=0)
junction support: 3 umis using 54 reads
cdr3 = CAWSMGQGRNYGYTF at 433, score = 7 + 9
umis assigned: [350, 392, 413, 444, 454, 717, 725, 963, 1085, 1208]
of which 10 are surviving nonsolos
reads assigned: 7981
start codons at 109, 112, 180, 445, 464
confident = true

TIG 2[bases=453]
0-20 ==> 36-56 on |529|TRAV9-2|5'UTR| [len=56] (mis=0)
20-358 ==> 0-338 on |530|TRAV9-2|L-REGION+V-REGION| [len=338] (mis=3)
354-411 ==> 0-57 on |416|TRAJ30|J-REGION| [len=57] (mis=4)
411-453 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 1 umis using 151 reads
cdr3 = CALSRRDDKIIF at 344, score = 9 + 8
umis assigned: [950, 1070]
of which 2 are surviving nonsolos
reads assigned: 1885
start codons at 20, 95, 183, 237, 363
confident = true
now this is a cell
paired!

TCAGTTCAAGTGTCAGACTCAGCGGTGTACTTCTGTGCTCTGAGTAGGAGAGATGACAAGATCATCTTTGGAAAAGGGACACGACTTCATATTCTCCCCA <==> CTTCTCAGTGACTCTGGCTTCTATCTCTGTGCCTGGAGTATGGGACAGGGGCGCAACTATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.615 = ACCCACTGTCGACTGC-1

using 82 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[81]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.623 = ACCCACTGTGAACCTT-1

using 21 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.628 = ACCCACTGTGTCGCTG-1

using 79 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2^2, 3, 4, 5, 12^2, 14, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.630 = ACCCACTGTTAAAGTG-1

using 42 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[42]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.632 = ACCCACTGTTATGCGT-1

using 64 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[29, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.641 = ACCCACTTCAAAGTAG-1

using 92 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.642 = ACCCACTTCAACGCTA-1

using 65 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.647 = ACCCACTTCACTTCAT-1

using 92 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 10[2^2, 3, 4, 7, 8, 10, 13, 16, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.648 = ACCCACTTCAGAGCTT-1

using 93 reads

====================================================================================

graph has 105 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 14[2^3, 3^2, 4^2, 5^2, 7, 10, 13^2, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.657 = ACCCACTTCCAGAAGG-1

using 43 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.658 = ACCCACTTCCAGTATG-1

using 83 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[83]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.659 = ACCCACTTCCATGAGT-1

using 95 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.665 = ACCCACTTCCTTGACC-1

using 52 reads

====================================================================================

graph has 32 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[26^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.666 = ACCCACTTCCTTTACA-1

using 113 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[18, 94]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.667 = ACCCACTTCCTTTCTC-1

using 61 reads

====================================================================================

graph has 28 edges initially, 4 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[24, 37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.671 = ACCCACTTCGCAGGCT-1

using 85 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.675 = ACCCACTTCGCGCCAA-1

using 86 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.676 = ACCCACTTCGCGTTTC-1

using 28 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.677 = ACCCACTTCGGAATCT-1

using 91 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[7^2, 8, 9, 11, 15, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.682 = ACCCACTTCGTTTATC-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.685 = ACCCACTTCTAACTTC-1

using 14 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.690 = ACCCACTTCTCGATGA-1

using 39 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 3, 31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.692 = ACCCACTTCTCTGTCG-1

using 430 reads

====================================================================================

graph has 250 edges initially, 16 edges after simplification

total ucounts = 21
nonsolo ucounts = 17[2^2, 3^2, 4, 5, 6, 10, 15, 20, 30, 33, 36, 39, 67, 69, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.694 = ACCCACTTCTGGTGTA-1

using 65 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 9, 48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.695 = ACCCACTTCTGTTTGT-1

using 133 reads

====================================================================================

graph has 54 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[44, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.696 = ACCCACTTCTTCCTTC-1

using 21 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[3^2, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.698 = ACCCACTTCTTGAGGT-1

using 61 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[4, 9, 10, 11, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.700 = ACCCACTTCTTGTATC-1

using 69 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 10[3^4, 4, 5, 7, 8^2, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.701 = ACCCACTTCTTTAGGG-1

using 59 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 8[3, 6^2, 7, 8^3, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.706 = ACCGTAAAGAATTGTG-1

using 31463 reads

====================================================================================

graph has 18517 edges initially, 784 edges after simplification

total ucounts = 1585
nonsolo ucounts = 1242[2^141, 3^95, 4^73, 5^54, 6^65, 7^55, 8^39, 9^33, 10^35, 11^23, 12^31, 13^34, 14^22, 15^38, 16^26, 17^24, 18^34, 19^23, 20^28, 21^19, 22^18, 23^24, 24^26, 25^16, 26^13, 27^21, 28^21, 29^22, 30^11, 31^11, 32^15, 33^5, 34^13, 35^15, 36^8, 37^10, 38^8, 39^6, 40^11, 41^9, 42^5, 43^9, 44^2, 45, 46^4, 47^4, 48^3, 49^3, 50, 51^3, 52^3, 53^2, 54, 55^3, 57, 58, 59, 61, 62^4, 63, 64^3, 65, 66, 70, 71, 77^2, 80, 85, 101, 105, 11477]
surviving nonsolo ucounts = 3[3, 39, 11477]
ids = [528, 1089, 951]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.709 = ACCGTAAAGACAGGCT-1

using 42208 reads

====================================================================================

graph has 12391 edges initially, 202 edges after simplification

total ucounts = 595
nonsolo ucounts = 433[2^73, 3^31, 4^30, 5^33, 6^21, 7^15, 8^23, 9^30, 10^11, 11^12, 12^9, 13^18, 14^12, 15^9, 16^5, 17^7, 18^8, 19^4, 20^6, 21^3, 22^6, 23, 24^6, 25^3, 26, 27^3, 28^4, 30^3, 31^3, 32^2, 33, 34^3, 35, 37, 38, 39, 41^2, 47^2, 49, 52, 54, 55, 56^2, 57, 58, 61, 73, 80, 85, 95, 127, 385, 865, 894, 1121, 1143, 1306, 1581, 1689, 1882, 2044, 2538, 3233, 5027, 5324, 8058]
surviving nonsolo ucounts = 15[385, 865, 894, 1121, 1143, 1306, 1581, 1689, 1882, 2044, 2538, 3233, 5027, 5324, 8058]
ids = [196, 373, 350, 549, 278, 519, 507, 396, 204, 61, ...]

====================================================================================

UMI info for barcode ACCGTAAAGACAGGCT-1 contig 1 = TGGGGAAGTG...
umi CATATCACAT = 386 reads: -190 +213 non-validated
umi CATTACACAC = 1930 reads: +145 -1XX +1 -6XX +2 -2XX +2 -1XX +1 -1XX +3 -76XX +1 -1XX +1 -4XX +2 -5XX +1 -7XX +140 invalidated
umi CTCCTCCCGT = 1153 reads: +403 validated
umi GCCAGACATA = 895 reads: +403 validated
umi GGCCCACGAC = 870 reads: +403 validated
umi TGATGCACAA = 1308 reads: +403 validated

UMI info for barcode ACCGTAAAGACAGGCT-1 contig 2 = TGGGGCTTCA...
umi ACCGGGTTGC = 2052 reads: +397 validated
umi ATGGGCCCCA = 5339 reads: -72X +325 invalidated
umi CATACCTCGG = 2569 reads: +397 validated
umi CCCACATTCA = 5049 reads: -72X +325 invalidated
umi CTCTTGCAAC = 8066 reads: -88 +1 -1XX +1 -1XX +305 invalidated
umi GTAGCTTCAC = 1521 reads: -381X +3 -5XX +1 -5XX +2 invalidated
umi TCAAGCTGCC = 3240 reads: +397 validated
umi TCTTGCCATT = 1445 reads: -377 +1 -3X +3 -5XX +1 -5XX +2 invalidated
umi TTACGGCGCG = 1120 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=603]
158-494 ==> 0-336 on |512|TRAV41|L-REGION+V-REGION| [len=336] (mis=4)
495-561 ==> 0-66 on |432|TRAJ45|J-REGION| [len=66] (mis=3)
561-603 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 6 umis using 268 reads
cdr3 = CAAEPSSGGGADGLTF at 482, score = 6 + 8
umis assigned: [196, 204, 278, 350, 373, 519]
of which 6 are surviving nonsolos
reads assigned: 6380
start codons at 29, 63, 158, 228, 365, 390
confident = true

TIG 2[bases=467]
18-362 ==> 0-344 on |613|TRBV28|L-REGION+V-REGION| [len=344] (mis=2)
368-415 ==> 0-47 on |550|TRBJ2-7|J-REGION| [len=47] (mis=2)
415-467 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 2300 reads
cdr3 = CASSFLAGAYEQYF at 345, score = 6 + 8
umis assigned: [61, 132, 194, 219, 287, 396, 457, 507, 549]
of which 9 are surviving nonsolos
reads assigned: 29929
start codons at 18, 76, 140, 153, 160, 168, 223, 226, 234, 336
confident = true
now this is a cell
paired!

AGAGACTCTGCCGTCTACATCTGTGCTGCCGAGCCCTCTTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCT <==> GCCAGCACCAACCAGACATCTATGTACCTCTGTGCCAGCAGTTTCTTGGCAGGGGCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.716 = ACCGTAAAGATAGTCA-1

using 26 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.722 = ACCGTAAAGCACGCCT-1

using 42569 reads

====================================================================================

graph has 13264 edges initially, 92 edges after simplification

total ucounts = 666
nonsolo ucounts = 472[2^92, 3^49, 4^44, 5^31, 6^29, 7^22, 8^28, 9^15, 10^16, 11^9, 12^20, 13^11, 14^10, 15^10, 16^14, 17^5, 18^6, 19^7, 20^4, 21^2, 22^4, 23^3, 24^2, 25, 26^2, 27^4, 28, 29, 32, 33^2, 34, 35^2, 45, 52, 55^2, 64, 66, 80, 84, 97, 347, 677, 759, 805, 850, 920, 1197, 1269, 1610, 2025, 2397, 3917, 5849, 7466, 8087]
surviving nonsolo ucounts = 15[347, 677, 759, 805, 850, 920, 1197, 1269, 1610, 2025, 2397, 3917, 5849, 7466, 8087]
ids = [134, 414, 391, 14, 19, 400, 277, 192, 549, 49, ...]

====================================================================================

UMI info for barcode ACCGTAAAGCACGCCT-1 contig 1 = AGTGACCCTG...
umi AATTCGGGGC = 1718 reads: +50 -5XX +1 -2XX +3 -2XX +1 -1XX +2 -14XX +1 -151X +1 -1XX +2 -1XX +1 -6XX +2 -3XX +1 -4XX +1 -1XX +143 invalidated
umi ACCACCTTCT = 5856 reads: -209 +2 -1XX +188 invalidated
umi ACGAACTTTA = 2028 reads: +400 validated
umi AGGTAGCCTT = 3884 reads: -252 +1 -2X +1 -1XX +143 invalidated
umi CAAAAGGGTA = 297 reads: -380 +1 -1XX +1 -1XX +5 -1XX +2 -1XX +2 -4XX +1 invalidated
umi CCCACTCGCA = 1285 reads: +400 validated
umi TCTGTGTAGG = 1604 reads: -282X +118 invalidated
umi TGCCGATCTT = 8125 reads: -137 +3 -2XX +258 invalidated

UMI info for barcode ACCGTAAAGCACGCCT-1 contig 2 = TTTTGAAACC...
umi AAGGAAATTC = 730 reads: +364 -5 +11 -1 +19 non-validated
umi AAGTCTGCCG = 792 reads: +361 -20 +19 non-validated
umi GATCAGGCCT = 7479 reads: -149X +251 invalidated
umi GGCAATCGCG = 742 reads: +400 validated
umi GGGACTTAAC = 837 reads: +400 validated
umi GTAATGCACT = 627 reads: +376 -9 +15 non-validated

GOOD CONTIGS

TIG 1[bases=497]
0-45 ==> 0-45 on |563|TRBV11-3|5'UTR| [len=45] (mis=0)
45-392 ==> 0-347 on |564|TRBV11-3|L-REGION+V-REGION| [len=347] (mis=2)
394-445 ==> 0-51 on |544|TRBJ2-2|J-REGION| [len=51] (mis=1)
445-497 ==> 0-52 on |534|TRBC2|C-REGION| [len=536] (mis=0)
junction support: 7 umis using 2062 reads
cdr3 = CASSLTRNTGELFF at 375, score = 9 + 9
umis assigned: [31, 42, 49, 90, 134, 192, 549, 573]
of which 8 are surviving nonsolos
reads assigned: 24269
start codons at 45, 247, 253
confident = true

TIG 2[bases=570]
0-128 ==> 0-128 on |523|TRAV8-4|5'UTR| [len=128] (mis=0)
128-469 ==> 0-341 on |524|TRAV8-4|L-REGION+V-REGION| [len=341] (mis=4)
468-528 ==> 0-60 on |441|TRAJ54|J-REGION| [len=60] (mis=3)
528-570 ==> 0-42 on |393|TRAC|C-REGION| [len=422] (mis=0)
junction support: 2 umis using 587 reads
cdr3 = CAVQVTQGAQKLVF at 455, score = 7 + 9
umis assigned: [14, 19, 331, 391, 400, 414]
of which 6 are surviving nonsolos
reads assigned: 10995
start codons at 128, 291, 431
confident = true

REJECT CONTIGS

TIG 1[bases=543]
5-180 ==> 34-209 on segment before TRAV39 exon 2 [len=209] (mis=1)
180-468 ==> 43-331 on |508|TRAV39|L-REGION+V-REGION| [len=331] (mis=2)
466-528 ==> 0-62 on |442|TRAJ56|J-REGION| [len=62] (mis=2)
cdr3 = CAVVHTGANSKLTF at 455, score = 6 + 8
umis assigned: [277]
of which 1 are surviving nonsolos
reads assigned: 1178
start codons at 41, 67, 93, 148, 163, 227, 345, 374, 432
confident = false
VJ delta = 24
not full
not full
now this is a cell
paired!

CATATGAGCGACGCGGCTGAGTACTTCTGTGCTGTGCAAGTGACCCAGGGAGCCCAGAAGCTGGTATTTGGCCAAGGAACCAGGCTGACTATCAACCCAA <==> GCAGAGCTTGGGGACTCGGCCGTGTATCTCTGTGCCAGCAGCTTAACCAGGAACACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTACTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.728 = ACCGTAAAGCTGAACG-1

using 31 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.732 = ACCGTAAAGGATGGAA-1

using 40 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.733 = ACCGTAAAGGATGGTC-1

using 4518 reads

====================================================================================

graph has 3802 edges initially, 136 edges after simplification

total ucounts = 544
nonsolo ucounts = 394[2^64, 3^50, 4^43, 5^31, 6^21, 7^20, 8^14, 9^18, 10^10, 11^11, 12^10, 13^9, 14^9, 15^8, 16^7, 17^4, 18^3, 19^5, 20, 21^5, 22^2, 23, 24, 25, 26^4, 27^4, 28^3, 29, 31^3, 32^2, 33^2, 34^2, 35, 37^2, 39, 40, 41^4, 42^2, 44, 45, 51, 52, 55, 56, 58, 63, 70, 76, 77, 79, 83, 88]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.735 = ACCGTAAAGGCACATG-1

using 102 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.736 = ACCGTAAAGGCTATCT-1

using 30 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.740 = ACCGTAAAGGGCATGT-1

using 444 reads

====================================================================================

graph has 338 edges initially, 14 edges after simplification

total ucounts = 19
nonsolo ucounts = 16[3, 7, 10, 11, 12, 13, 14, 15, 16, 35, 37, 38, 48, 56, 61, 65]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.741 = ACCGTAAAGGGTCGAT-1

using 58 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.742 = ACCGTAAAGGTAGCTG-1

using 108 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[6, 20, 82]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.745 = ACCGTAAAGTACCGGA-1

using 74 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[7, 8, 12, 46]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.747 = ACCGTAAAGTAGGTGC-1

using 86 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[85]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.761 = ACCGTAACAAAGCAAT-1

using 23 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.765 = ACCGTAACAACTGGCC-1

using 67 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.768 = ACCGTAACAATCACAC-1

using 86 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[86]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.769 = ACCGTAACAATCTGCA-1

using 96 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[96]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.773 = ACCGTAACACAACTGT-1

using 10 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.775 = ACCGTAACACAGACAG-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.776 = ACCGTAACACAGACTT-1

using 20 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.778 = ACCGTAACACAGCGTC-1

using 25 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.779 = ACCGTAACACATCTTT-1

using 48 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[48]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.783 = ACCGTAACACCTGGTG-1

using 29 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.788 = ACCGTAACAGACAAAT-1

using 18 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.790 = ACCGTAACAGATGAGC-1

using 32 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.794 = ACCGTAACAGCTTAAC-1

using 68 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[68]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.795 = ACCGTAACAGGAATGC-1

using 80 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 6, 9, 15, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.796 = ACCGTAACAGGGATTG-1

using 53 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 5, 43]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.16
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk05-ucc6a7a72e6/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_T_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk05-ucc6a7a72e6/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

98.600 seconds used processing barcodes, peak mem = 0.40
