[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.36 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk106-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk106-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk106.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.0 = TTCGAAGCATATGAGA-1

using 380 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 11^2, 350]
surviving nonsolo ucounts = 1[350]
ids = [5]

====================================================================================

UMI info for barcode TTCGAAGCATATGAGA-1 contig 1 = GAGTCAGACT...
umi GCCCCAGACC = 347 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
376-413 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYNSYPLTF at 352, score = 8 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 343
start codons at 25, 31, 87, 100, 236, 239, 332, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.4 = TTCGAAGCATGCAATC-1

using 9932 reads

====================================================================================

graph has 5006 edges initially, 42 edges after simplification

total ucounts = 636
nonsolo ucounts = 326[2^113, 3^61, 4^37, 5^32, 6^16, 7^11, 8^5, 9^3, 10^5, 11^3, 12, 13^4, 14, 16^2, 18, 30, 34, 48, 64, 77, 145, 181, 186, 212, 242, 244, 250, 251, 252, 275, 276, 279, 280, 281, 291, 298, 302, 310, 311, 325, 330, 340, 368, 537, 670, 765]
surviving nonsolo ucounts = 28[34, 64, 145, 181, 186, 212, 242, 244, 250, 251, 252, 275, 276, 279, 280, 281, 291, 298, 302, 310, 311, 325, 330, 340, 368, 537, 670, 765]
ids = [399, 327, 3, 125, 153, 262, 100, 201, 302, 561, ...]

====================================================================================

UMI info for barcode TTCGAAGCATGCAATC-1 contig 1 = AGCTCTGAGA...
umi AAACACTCCT = 147 reads: +421 validated
umi AACTGACCGG = 319 reads: +421 validated
umi CATCGAGCTG = 767 reads: +421 validated
umi CTTATTTTGG = 228 reads: +421 validated
umi GACCCTCTGG = 55 reads: +350 -1 +7 -63 non-validated

UMI info for barcode TTCGAAGCATGCAATC-1 contig 2 = GGGGAGGAGT...
umi AACTGGGTCT = 308 reads: +388 validated
umi ACGTCGACCG = 276 reads: +388 validated
umi AGAATTGGGT = 278 reads: +388 validated
umi AGACAGGCTG = 294 reads: +388 validated
umi AGCAAAGTAC = 239 reads: +388 validated
umi AGCTTCAAGA = 682 reads: +388 validated
umi ATAAAGATGG = 183 reads: +388 validated
umi CCTCTCGGAC = 255 reads: +388 validated
umi CGTCGGCGCA = 212 reads: +388 validated
umi GACCCACCTC = 280 reads: +388 validated
umi GGCTTCGTTA = 307 reads: +188 -1XX +199 invalidated
umi TAGGTCGCTC = 274 reads: +388 validated
umi TATAAACGGT = 369 reads: +388 validated
umi TCCCTCCGTA = 328 reads: +388 validated
umi TCGGCAATCC = 332 reads: +388 validated
umi TCTATTGGCC = 324 reads: +388 validated
umi TCTTCGCGTA = 539 reads: -117X +271 invalidated
umi TTAAGGTCCC = 244 reads: +388 validated
umi TTCGAGGCCG = 317 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=602]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=2)
448-500 ==> 11-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
500-602 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 4 umis using 130 reads
cdr3 = CAKAKVTSSYYGMDVW at 421, score = 9 + 7
umis assigned: [3, 24, 214, 302, 327]
of which 5 are surviving nonsolos
reads assigned: 1475
start codons at 79, 230, 235, 382, 457, 518, 579
confident = true

TIG 2[bases=555]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
381-419 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 19 umis using 1004 reads
cdr3 = CQQYDNLPLTF at 358, score = 9 + 9
umis assigned: [27, 80, 96, 97, 100, 106, 125, 234, 262, 326] and 9 others
of which 19 are surviving nonsolos
reads assigned: 5934
start codons at 31, 37, 93, 106, 245, 368, 461
confident = true
now this is a cell
paired!

GAGGACACGGCCGTATATTACTGTGCGAAAGCGAAGGTGACTTCCTCATACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.6 = TTCGAAGCATTAGCCA-1

using 15881 reads

====================================================================================

graph has 12212 edges initially, 230 edges after simplification

total ucounts = 2024
nonsolo ucounts = 1502[2^240, 3^164, 4^163, 5^144, 6^120, 7^107, 8^102, 9^83, 10^67, 11^56, 12^42, 13^35, 14^36, 15^31, 16^20, 17^15, 18^12, 19^14, 20^5, 21^12, 22^2, 23^2, 24^2, 25, 27, 29, 38, 55, 61, 94, 99, 104^2, 113, 151, 163^2, 182, 190, 216, 238, 256, 284, 291, 292, 308, 309, 315, 344, 381, 389]
surviving nonsolo ucounts = 21[38, 61, 99, 104^2, 151, 163^2, 182, 216, 238, 256, 284, 291, 292, 308, 309, 315, 344, 381, 389]
ids = [850, 1920, 1188, 1435, 1857, 1972, 333, 1139, 1180, 1156, ...]

====================================================================================

UMI info for barcode TTCGAAGCATTAGCCA-1 contig 1 = ATGGGGGATC...
umi AGCGTTTTCT = 164 reads: +394 validated
umi ATCTTATTGT = 297 reads: +394 validated
umi CACATTCCAC = 345 reads: +394 validated
umi CATAGACGTC = 307 reads: +394 validated
umi CCTCTCCGTT = 387 reads: +394 validated
umi CCTGTAATAC = 38 reads: -4 +384 -1 +5 non-validated
umi CGTAGTATAT = 293 reads: +394 validated
umi CTGGATATTG = 310 reads: +394 validated
umi GTGTTTTACA = 238 reads: +394 validated
umi GTTACGAATG = 287 reads: +394 validated
umi TAAACTTATA = 381 reads: +394 validated
umi TTCATACAGT = 61 reads: +376 -2 +1 -1 +5 -9 non-validated
umi TTGCCTTGCC = 154 reads: +394 validated

UMI info for barcode TTCGAAGCATTAGCCA-1 contig 2 = GAGCTCTGGG...
umi CTTGGAAAGG = 154 reads: +461 -5 non-validated
umi CTTTTGTCCT = 215 reads: +422 -1 +43 non-validated
umi GAATCCTTGT = 185 reads: +407 -1 +8 -1 +19 -1 +9 -2 +7 -1 +1 -9 non-validated
umi GACAGCTACT = 98 reads: +405 -1 +2 -2 +1 -1 +1 -1 +39 -13 non-validated

GOOD CONTIGS

TIG 1[bases=566]
0-36 ==> 0-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=4)
36-396 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=1)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 12 umis using 525 reads
cdr3 = CMQALQTPLF at 372, score = 9 + 7
umis assigned: [333, 461, 568, 644, 833, 850, 957, 1091, 1483, 1492] and 3 others
of which 13 are surviving nonsolos
reads assigned: 3207
start codons at 0, 36, 69, 105, 193, 355, 375, 472
confident = true

TIG 2[bases=648]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=5)
454-485 ==> 0-31 on |14|IGHD2-2|D-REGION| [len=31] (mis=8)
500-546 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
546-648 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 0 umis using 0 reads
cdr3 = CAKVLSIGTPDSYCSSTSCHGYSYGGGLDYW at 422, score = 9 + 7
umis assigned: [1139, 1156, 1180, 1188]
of which 4 are surviving nonsolos
reads assigned: 640
start codons at 80, 231, 236, 294, 297, 315, 383, 492, 564, 625
confident = true

REJECT CONTIGS

TIG 1[bases=544]
1-345 ==> 1142-1486 on rc of segment before IGHD5-12 exon 1 [len=1486] (mis=0)
379-442 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
442-544 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [1419, 1857]
of which 2 are surviving nonsolos
reads assigned: 411
start codons at 55, 70, 94, 399, 460, 521
confident = false
did not find CDR3
now this is a cell
paired!

ACGCCCGATTCGTATTGTAGTAGTACCAGCTGCCACGGATACAGCTATGGGGGGGGTCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AAAATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCCCCTTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.16 = TTCGAAGGTAGAGGAA-1

using 329 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[329]
surviving nonsolo ucounts = 1[329]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGGTAGAGGAA-1 contig 1 = GGCTGGGGTC...
umi TTCTGGGCGC = 327 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=641]
0-42 ==> 0-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=1)
42-370 ==> 0-328 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=16)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
430-641 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CSSVAGSYSLVF at 366, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 324
start codons at 42, 181, 199, 250, 253, 349
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.21 = TTCGAAGGTAGTAGTA-1

using 740 reads

====================================================================================

graph has 312 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 5, 222, 244, 256]
surviving nonsolo ucounts = 3[222, 244, 256]
ids = [8, 3, 10]

====================================================================================

UMI info for barcode TTCGAAGGTAGTAGTA-1 contig 1 = AGGAATCAGT...
umi GTTTAAATGG = 220 reads: +388 validated
umi TATCATATGG = 251 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 79 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [8, 10]
of which 2 are surviving nonsolos
reads assigned: 465
start codons at 27, 33, 102, 238, 457
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.29 = TTCGAAGGTCTGATCA-1

using 61 reads

====================================================================================

graph has 76 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[3, 4, 5, 6, 7, 8, 12^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.30 = TTCGAAGGTCTGATTG-1

using 243 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 239]
surviving nonsolo ucounts = 1[239]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGGTCTGATTG-1 contig 1 = GGTCTGCTTC...
umi AAAATTGCTC = 241 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=582]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=1)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-582 ==> 0-137 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 234
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.38 = TTCGAAGGTGGAAAGA-1

using 20547 reads

====================================================================================

graph has 7215 edges initially, 68 edges after simplification

total ucounts = 663
nonsolo ucounts = 321[2^101, 3^52, 4^35, 5^21, 6^10, 7^7, 8^7, 9^5, 10^2, 11, 12, 13, 14^2, 15, 19, 21, 34, 36, 40, 50, 72, 91, 93, 102, 107, 124, 128, 140, 143, 150, 151, 153, 158, 165, 172, 190, 191, 193, 196, 197, 202, 204^2, 207, 208, 209, 212, 214, 215, 219, 221, 224, 229, 231, 232^2, 235, 237, 245, 248, 249, 252, 254, 255, 257, 260, 264, 268, 275, 283, 287^2, 299, 311, 312, 315, 317, 320, 327, 332, 333, 371, 438, 448, 547, 549, 996, 1010, 1333]
surviving nonsolo ucounts = 69[50, 72, 91, 93, 102, 107, 124, 128, 140, 143, 150, 151, 153, 158, 165, 172, 190, 191, 193, 196, 197, 202, 204^2, 207, 208, 209, 212, 214, 215, 219, 221, 224, 229, 231, 232^2, 235, 237, 245, 248, 249, 252, 254, 255, 257, 260, 264, 268, 275, 283, 287^2, 299, 311, 312, 315, 317, 320, 332, 333, 371, 438, 448, 547, 549, 996, 1010, 1333]
ids = [241, 217, 277, 392, 86, 140, 524, 410, 67, 166, ...]

====================================================================================

UMI info for barcode TTCGAAGGTGGAAAGA-1 contig 1 = CTGGGCCTCA...
umi AACAAGTCGA = 257 reads: +376 validated
umi ACATTACCAG = 283 reads: +376 validated
umi AGGAAGGAAA = 326 reads: -15X +361 invalidated
umi AGTAGTCACT = 294 reads: +376 validated
umi CAGCCCTCTG = 320 reads: +376 validated
umi CAGTACCTCG = 297 reads: +376 validated
umi CATCGCCTGA = 564 reads: -342X +2 -1XX +5 -4XX +9 -1XX +1 -1XX +10 invalidated
umi CCCCTGCGAG = 209 reads: +376 validated
umi CCCGCAAGAT = 261 reads: +376 validated
umi CCGAATGCCT = 167 reads: +376 validated
umi CGACATTCCT = 226 reads: +376 validated
umi CGATCCCCGA = 268 reads: +376 validated
umi CTACTTCGGT = 230 reads: +376 validated
umi CTACTTTATT = 275 reads: +376 validated
umi GCCTCTTTCG = 247 reads: +376 validated
umi GTACCCATCT = 1354 reads: -336X +1 -1XX +1 -3XX +2 -1XX +5 -4XX +9 -1XX +1 -1XX +10 invalidated
umi GTGTTGGTCA = 189 reads: +376 validated
umi GTTCCGGTGT = 322 reads: +376 validated
umi TAATGAAGAC = 210 reads: +196 -1XX +179 invalidated
umi TATACTCCCA = 234 reads: +376 validated
umi TCAAGCCTGA = 216 reads: +376 validated
umi TCATAGCTCA = 221 reads: +376 validated
umi TGATGTGAGG = 281 reads: +376 validated
umi TGGTTTCCCC = 456 reads: -150 +1 -3X +222 invalidated

UMI info for barcode TTCGAAGGTGGAAAGA-1 contig 2 = GGAGTCTCCC...
umi AAGACAAGCC = 260 reads: +424 validated
umi AAGATGCGCT = 149 reads: +424 validated
umi AGTCAACATG = 141 reads: +424 validated
umi ATATCTCTTA = 938 reads: -389X +1 -1XX +1 -6XX +1 -6XX +2 -7XX +1 -6XX +2 -1XX invalidated
umi ATATTATAGG = 104 reads: +424 validated
umi CAATTACCGG = 63 reads: -26X +1 -1XX +1 -1XX +3 -1XX +4 -4XX +1 -1XX +3 -1XX +2 -5XX +2 -1XX +22 -1XX +2 -1XX +7 -3XX +4 -1XX +4 -2XX +2 -2XX +5 -1XX +1 -277 +4 -2XX +2 -1XX +5 -1XX +5 -1XX +8 -1XX +1 invalidated
umi CACCCATCCG = 938 reads: -389X +1 -1XX +1 -6XX +1 -6XX +2 -7XX +1 -6XX +2 -1XX invalidated
umi CACCGAGTAC = 205 reads: +424 validated
umi CAGTTATCCG = 110 reads: +424 validated
umi CATATAGGCT = 239 reads: +424 validated
umi CATATGTATG = 196 reads: +424 validated
umi CCAACGACAA = 144 reads: +424 validated
umi CCAAGTATTA = 260 reads: +424 validated
umi CCATGCTAGT = 240 reads: +424 validated
umi CCATTCACCC = 191 reads: +424 validated
umi CCCATAGTAG = 233 reads: +424 validated
umi CCGAAGCATC = 238 reads: +424 validated
umi CCGGTACCCT = 70 reads: +424 validated
umi CCTGTATTTG = 195 reads: +424 validated
umi CCTTGTGGCA = 50 reads: +299 -1 +1 -1 +4 -4 +11 -1 +5 -1 +26 -1 +22 -1 +46 non-validated
umi CGACCACTTA = 210 reads: +424 validated
umi CTAACGTCAG = 160 reads: +424 validated
umi CTACATCACT = 91 reads: +424 validated
umi CTACATGAGT = 212 reads: +424 validated
umi CTATGACTCG = 248 reads: +424 validated
umi CTCCATTCAT = 267 reads: +424 validated
umi GCAGATCGAA = 220 reads: +424 validated
umi GCAGCTCCAT = 231 reads: +424 validated
umi GCGTTACATT = 199 reads: +424 validated
umi GTCAATACCT = 129 reads: +424 validated
umi TATTGAATTG = 207 reads: +424 validated
umi TCCCTTTTTT = 122 reads: +424 validated
umi TCCTGCGGGT = 209 reads: +424 validated
umi TCGGCAATAG = 153 reads: +424 validated
umi TGGCAACCTT = 513 reads: -389 +1 -1X +1 -6XX +1 -6XX +2 -7XX +1 -6XX +2 -1XX invalidated
umi TGTTTAAGCG = 153 reads: +424 validated
umi TTTCTAATGA = 175 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=624]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-373 ==> 0-336 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=5)
375-413 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
413-624 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 22 umis using 1120 reads
cdr3 = CQAWDSSSYVF at 352, score = 6 + 8
umis assigned: [1, 22, 59, 66, 137, 138, 151, 195, 196, 207] and 14 others
of which 24 are surviving nonsolos
reads assigned: 7473
start codons at 37, 42, 98, 185, 331, 335, 377, 545
confident = true

TIG 2[bases=643]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=7)
435-483 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
483-643 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 33 umis using 615 reads
cdr3 = CARHQGNDYGEKPFDYW at 401, score = 8 + 7
umis assigned: [5, 8, 67, 84, 86, 118, 123, 125, 140, 145] and 27 others
of which 36 are surviving nonsolos
reads assigned: 8316
start codons at 59, 257, 392, 420, 537
confident = true

REJECT CONTIGS

TIG 1[bases=570]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-44 ==> 5956-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=8)
397-434 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
434-570 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [3, 7, 69, 179, 271, 405, 498, 508]
of which 8 are surviving nonsolos
reads assigned: 2514
start codons at 44, 252, 378, 476
confident = false
did not find CDR3
now this is a cell
paired!

GACACCGCCATGTATTACTGTGCGAGACACCAAGGGAATGACTACGGTGAAAAACCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCGGGACCCAGGCTATGGATGAGGCTGACTATTATTGTCAGGCGTGGGACAGCAGCTCTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.40 = TTCGAAGGTGTTTGTG-1

using 267 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[267]
surviving nonsolo ucounts = 1[267]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGGTGTTTGTG-1 contig 1 = AGCTCTCAGA...
umi CTCACTTACC = 251 reads: +454 validated

GOOD CONTIGS

TIG 1[bases=549]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=1)
470-533 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CARVGHIHFYSSGWSTYYYYYYGMDVW at 421, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 79, 230, 235, 382, 490
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.43 = TTCGAAGGTTCCACAA-1

using 220 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 212]
surviving nonsolo ucounts = 1[212]
ids = [1]

====================================================================================

UMI info for barcode TTCGAAGGTTCCACAA-1 contig 1 = ATACTTTCTG...
umi CGAGCACGAC = 199 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=572]
0-37 ==> 64-101 on |195|IGHV4-61|5'UTR| [len=101] (mis=0)
37-393 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=52)
422-470 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
470-572 ==> 0-102 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CVRGADYTFFYSSGYFHSW at 382, score = 9 + 6
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 37, 81, 524
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.45 = TTCGAAGGTTCCGTCT-1

using 362 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 11, 343]
surviving nonsolo ucounts = 2[11, 343]
ids = [3, 4]

====================================================================================

UMI info for barcode TTCGAAGGTTCCGTCT-1 contig 1 = GAAGAGCTGC...
umi GCATTTTCCG = 11 reads: -60 +56 -65 +7 -1 +124 -27 +48 non-validated
umi GCCGAATGCT = 347 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=557]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYGSSPPYTF at 357, score = 9 + 8
umis assigned: [3, 4]
of which 2 are surviving nonsolos
reads assigned: 350
start codons at 33, 241, 367, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.46 = TTCGAAGGTTCTCATT-1

using 262 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 4, 255]
surviving nonsolo ucounts = 1[255]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGGTTCTCATT-1 contig 1 = GTGGGCTCAG...
umi AACAGACCAT = 248 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=582]
0-36 ==> 4-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
36-373 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=16)
377-415 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=6)
415-582 ==> 0-167 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CNSRARTGDHVF at 351, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 240
start codons at 36, 118, 155, 184, 231, 235, 334, 379, 547
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.47 = TTCGAAGGTTGGAGGT-1

using 199 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 193]
surviving nonsolo ucounts = 1[193]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGGTTGGAGGT-1 contig 1 = TCTCCCTCAC...
umi AACCTCTACT = 163 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=480]
0-55 ==> 4-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=0)
55-408 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=6)
425-473 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
junction support: 1 umis using 14 reads
cdr3 = CASRLRFFDWSFDYW at 397, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 55, 229, 253, 388
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.49 = TTCGAAGTCAATCACG-1

using 40 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.52 = TTCGAAGTCACCCTCA-1

using 381 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 4, 10, 362]
surviving nonsolo ucounts = 1[362]
ids = [5]

====================================================================================

UMI info for barcode TTCGAAGTCACCCTCA-1 contig 1 = GGAACTGCTC...
umi TATATCGCGC = 366 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=549]
0-31 ==> 16-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=1)
31-376 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=7)
375-413 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQRSDWPVTF at 352, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 360
start codons at 31, 236, 239, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.55 = TTCGAAGTCACTTATC-1

using 626 reads

====================================================================================

graph has 246 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[6, 31, 284, 299]
surviving nonsolo ucounts = 2[284, 299]
ids = [7, 1]

====================================================================================

UMI info for barcode TTCGAAGTCACTTATC-1 contig 1 = AGGAGTCAGT...
umi ACGTATGAGC = 265 reads: +388 validated

UMI info for barcode TTCGAAGTCACTTATC-1 contig 2 = GCTGGGGTCT...
umi TAATCTTGCG = 268 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=478]
0-27 ==> 20-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
415-478 ==> 0-63 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQSYRRPITF at 354, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 27, 33, 89, 102, 238, 337, 457
confident = false

TIG 2[bases=585]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-356 ==> 0-315 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=12)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
429-585 ==> 0-156 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 51 reads
cdr3 = CTSYAGGSNVVF at 365, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 41, 249, 348, 375, 390
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.57 = TTCGAAGTCAGGTTCA-1

using 168 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[163]
surviving nonsolo ucounts = 1[163]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGTCAGGTTCA-1 contig 1 = AGTCCCACTC...
umi AACTACCTTC = 149 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=493]
0-20 ==> 7-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
20-371 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
408-493 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CLQYSSSPWTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 147
start codons at 20, 26, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.70 = TTCGAAGTCGGAAACG-1

using 279 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2^3, 7, 265]
surviving nonsolo ucounts = 1[265]
ids = [4]

====================================================================================

UMI info for barcode TTCGAAGTCGGAAACG-1 contig 1 = GTGGGCTCAG...
umi CAACGTCACA = 255 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=557]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=10)
379-417 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
417-557 ==> 0-140 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CYSTDSSGNRRVF at 350, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 252
start codons at 35, 96, 165, 183, 234, 296, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.74 = TTCGAAGTCGTACCGG-1

using 246 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3^2, 21, 213]
surviving nonsolo ucounts = 2[3, 213]
ids = [5, 8]

====================================================================================

UMI info for barcode TTCGAAGTCGTACCGG-1 contig 1 = GATCAGGACT...
umi GCACAAGGCG = 3 reads: -397 non-validated
umi TGAACCAGTG = 214 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=2)
389-427 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CMQALQTRETF at 366, score = 9 + 8
umis assigned: [5, 8]
of which 2 are surviving nonsolos
reads assigned: 212
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.76 = TTCGAAGTCTAACTCT-1

using 318 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3^3, 305]
surviving nonsolo ucounts = 1[305]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGTCTAACTCT-1 contig 1 = AGGAGTCAGA...
umi GCACCATCTG = 283 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=505]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=2)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-505 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQYNSYWTF at 354, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 280
start codons at 27, 33, 89, 102, 334, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.82 = TTCGAAGTCTCGATGA-1

using 412 reads

====================================================================================

graph has 192 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2^2, 402]
surviving nonsolo ucounts = 1[402]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=364]
0-34 ==> 128-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
6-61 ==> 5937-5992 on segment before IGLV2-5 exon 1 [len=6137] (mis=2)
15-81 ==> 5809-5875 on segment before IGLV2-34 exon 1 [len=6000] (mis=3)
34-327 ==> 0-293 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=22)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 343
start codons at 34, 170, 173, 188, 191
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 106.84 = TTCGAAGTCTCTTGAT-1

using 181 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[5, 6, 170]
surviving nonsolo ucounts = 1[170]
ids = [0]

====================================================================================

UMI info for barcode TTCGAAGTCTCTTGAT-1 contig 1 = AGTCCCACTC...
umi ACATGATGAG = 168 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 7-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
20-371 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CLQYSSSPWTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 167
start codons at 20, 26, 95, 231, 450
confident = false
NOT paired!

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BARCODE 106.85 = TTCGAAGTCTGAAAGA-1

using 667 reads

====================================================================================

graph has 222 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3^2, 241, 414]
surviving nonsolo ucounts = 2[241, 414]
ids = [0, 7]

====================================================================================

UMI info for barcode TTCGAAGTCTGAAAGA-1 contig 1 = GAGAAGAGCT...
umi CTTGGATGGT = 409 reads: +385 validated

UMI info for barcode TTCGAAGTCTGAAAGA-1 contig 2 = AGCTTCAGCT...
umi ACGTCCTTTG = 236 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
382-420 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CQQYGSSLFTF at 359, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 407
start codons at 35, 243, 369, 462
confident = false

TIG 2[bases=543]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
397-435 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
435-543 ==> 0-108 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CAAWDDSLNGYVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 47, 351, 376, 381, 393, 399
confident = false
NOT paired!

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BARCODE 106.86 = TTCGAAGTCTGGAGCC-1

using 479 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[230, 246]
surviving nonsolo ucounts = 2[230, 246]
ids = [2, 1]

====================================================================================

UMI info for barcode TTCGAAGTCTGGAGCC-1 contig 1 = AGCTTCAGCT...
umi CCTCCTCTAC = 248 reads: +388 validated
umi CCTTCCAGCG = 231 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=13)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 95 reads
cdr3 = CAAWDASLNGWVF at 368, score = 8 + 8
umis assigned: [1, 2]
of which 2 are surviving nonsolos
reads assigned: 468
start codons at 47, 252, 329, 351, 376, 381, 393
confident = false
NOT paired!

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BARCODE 106.94 = TTCGGTCAGACCTTTG-1

using 328 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 5, 6, 311]
surviving nonsolo ucounts = 1[311]
ids = [4]

====================================================================================

UMI info for barcode TTCGGTCAGACCTTTG-1 contig 1 = GGGGAGGAAC...
umi GCGGCATTAC = 270 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=511]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=13)
383-421 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
421-511 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CHQRSNWPPWTF at 357, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 266
start codons at 36, 241, 244, 463
confident = false
NOT paired!
sorting bam, mem = 0.07
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk106-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk106-ucc6a7a72ce/files/contig_bam.bam

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10.292 seconds used processing barcodes, peak mem = 0.23
