[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.18 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk101-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk101-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk101.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.0 = TGCGTGGAGGGTCTCC-1

using 184 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 175]
surviving nonsolo ucounts = 1[175]
ids = [3]

====================================================================================

UMI info for barcode TGCGTGGAGGGTCTCC-1 contig 1 = AGGAACTGCT...
umi CCCAGCTCGG = 153 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=484]
0-32 ==> 15-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
32-377 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=17)
382-420 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
420-484 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQHRSDWPPIFTF at 353, score = 10 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 32, 237, 240, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.3 = TGCGTGGAGGTGCTAG-1

using 324 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2^2, 3, 311]
surviving nonsolo ucounts = 1[311]
ids = [5]

====================================================================================

UMI info for barcode TGCGTGGAGGTGCTAG-1 contig 1 = GATCAGGACT...
umi GGCTTCACGT = 302 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=519]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=3)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-519 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 300
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.9 = TGCGTGGAGTGGGCTA-1

using 1022 reads

====================================================================================

graph has 1040 edges initially, 28 edges after simplification

total ucounts = 387
nonsolo ucounts = 143[2^56, 3^35, 4^19, 5^14, 6^6, 7^4, 8^3, 11, 13, 14, 15, 17, 257]
surviving nonsolo ucounts = 1[257]
ids = [368]

====================================================================================

UMI info for barcode TGCGTGGAGTGGGCTA-1 contig 1 = GAGGAATCAG...
umi TTCTCGTAAC = 259 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [368]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.12 = TGCGTGGAGTGTTAGA-1

using 628 reads

====================================================================================

graph has 244 edges initially, 8 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 3, 7, 126, 220, 266]
surviving nonsolo ucounts = 2[220, 266]
ids = [9, 1]

====================================================================================

UMI info for barcode TGCGTGGAGTGTTAGA-1 contig 1 = AGGAACTGCT...
umi CCCACACAGG = 264 reads: +382 validated

UMI info for barcode TGCGTGGAGTGTTAGA-1 contig 2 = GGAGTCAGTC...
umi TTTTATCGTG = 218 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=550]
0-32 ==> 15-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
32-377 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQRSNWPLTF at 353, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 259
start codons at 32, 240, 456
confident = false

TIG 2[bases=550]
32-280 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CLHYDNRRRTF at 353, score = 9 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 217
start codons at 32, 88, 101, 240, 363, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.17 = TGCGTGGCAACAACCT-1

using 279 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[5, 270]
surviving nonsolo ucounts = 1[270]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.27 = TGCGTGGCACCATCCT-1

using 227 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[226]
surviving nonsolo ucounts = 1[226]
ids = [0]

====================================================================================

UMI info for barcode TGCGTGGCACCATCCT-1 contig 1 = GAGTCAGTCC...
umi ACGGCACCCA = 227 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=555]
0-25 ==> 6-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
25-376 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQYDNLPVSLTF at 352, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 25, 31, 87, 100, 239, 362, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.31 = TGCGTGGCACTATCTT-1

using 322 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 3, 311]
surviving nonsolo ucounts = 1[311]
ids = [5]

====================================================================================

UMI info for barcode TGCGTGGCACTATCTT-1 contig 1 = AGCTCTGAGA...
umi TCGCATGGGT = 296 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=584]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-584 ==> 0-81 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.33 = TGCGTGGCACTCTGTC-1

using 309 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[307]
surviving nonsolo ucounts = 1[307]
ids = [1]

====================================================================================

UMI info for barcode TGCGTGGCACTCTGTC-1 contig 1 = GGGGTCACAA...
umi AGACTGCCAT = 309 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=640]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-331 ==> 0-293 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=22)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=8)
429-640 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CSSYAGRTTFDVF at 362, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 38, 174, 177, 192, 195, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.48 = TGCGTGGCATGTTGAC-1

using 29 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 1[28]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.51 = TGCGTGGGTAAGAGAG-1

using 158 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 4, 146]
surviving nonsolo ucounts = 1[146]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.53 = TGCGTGGGTACTCGCG-1

using 144 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[144]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.69 = TGCGTGGGTCGCGAAA-1

using 194 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[187]
surviving nonsolo ucounts = 1[187]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.73 = TGCGTGGGTGATGCCC-1

using 789 reads

====================================================================================

graph has 326 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 6[2^3, 3, 266, 502]
surviving nonsolo ucounts = 2[266, 502]
ids = [6, 8]

====================================================================================

UMI info for barcode TGCGTGGGTGATGCCC-1 contig 1 = GAATCAGTCC...
umi CCCTTGTGCA = 266 reads: +388 validated
umi GGGTTAACTC = 511 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 138 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [6, 8]
of which 2 are surviving nonsolos
reads assigned: 758
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.80 = TGCGTGGGTGTTGAGG-1

using 28 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.85 = TGCGTGGGTTACTGAC-1

using 430 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 135, 289]
surviving nonsolo ucounts = 2[135, 289]
ids = [6, 3]

====================================================================================

UMI info for barcode TGCGTGGGTTACTGAC-1 contig 1 = AGACTCAGTC...
umi CTTTACGAGT = 288 reads: +388 validated
umi TGTGTAGTCC = 135 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
20-371 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 57 reads
cdr3 = CQQYNSYPLTF at 347, score = 8 + 9
umis assigned: [3, 6]
of which 2 are surviving nonsolos
reads assigned: 422
start codons at 20, 26, 82, 95, 231, 234, 327, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.95 = TGCGTGGTCACCATAG-1

using 362 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 5, 6, 7, 8, 328]
surviving nonsolo ucounts = 1[328]
ids = [0]

====================================================================================

UMI info for barcode TGCGTGGTCACCATAG-1 contig 1 = AGAGCTCTGG...
umi AATGGTGGTA = 307 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=494]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
389-426 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
426-494 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYGSSPTF at 368, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 44, 252, 378, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.96 = TGCGTGGTCACCGTAA-1

using 269 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 7, 10, 242]
surviving nonsolo ucounts = 1[242]
ids = [2]

====================================================================================

UMI info for barcode TGCGTGGTCACCGTAA-1 contig 1 = AGAAGCAGAG...
umi CGTCTTCGCT = 233 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=517]
0-28 ==> 12-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
28-365 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
366-404 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
404-517 ==> 0-113 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CNSRDSSGWVF at 343, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 28, 147, 176, 227, 326
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.102 = TGCGTGGTCCACGAAT-1

using 42 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 5, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.105 = TGCGTGGTCCCGACTT-1

using 333 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 4, 324]
surviving nonsolo ucounts = 1[324]
ids = [5]

====================================================================================

UMI info for barcode TGCGTGGTCCCGACTT-1 contig 1 = GGAGTCAGTC...
umi GGACCTGGTT = 320 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 21-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CQQSYRRPITF at 353, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 317
start codons at 26, 32, 88, 101, 237, 336, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.115 = TGCGTGGTCGAATCCA-1

using 1848 reads

====================================================================================

graph has 2252 edges initially, 60 edges after simplification

total ucounts = 953
nonsolo ucounts = 373[2^165, 3^96, 4^49, 5^24, 6^11, 7^13, 8, 9^3, 10^3, 11^3, 12, 13, 14, 15, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.116 = TGCGTGGTCGCAAGCC-1

using 3005 reads

====================================================================================

graph has 2170 edges initially, 28 edges after simplification

total ucounts = 355
nonsolo ucounts = 212[2^37, 3^24, 4^30, 5^18, 6^14, 7^12, 8^10, 9^7, 10^10, 11^10, 12^8, 13^2, 14^10, 15^4, 16, 17^2, 18^4, 19^2, 40, 176, 193, 228, 274, 276, 315]
surviving nonsolo ucounts = 7[18, 176, 193, 228, 274, 276, 315]
ids = [29, 276, 216, 348, 139, 33, 121]

====================================================================================

UMI info for barcode TGCGTGGTCGCAAGCC-1 contig 1 = AGAGCTCTGG...
umi ACCATGCGCA = 282 reads: +385 validated
umi GCTCACCATC = 195 reads: +385 validated

UMI info for barcode TGCGTGGTCGCAAGCC-1 contig 2 = AGCTCTGAGA...
umi ACAGCGTTCA = 17 reads: -39 +59 -1 +130 -42 +60 -35 +52 non-validated
umi CACTTCCCGT = 315 reads: +418 validated
umi CCGCGCTTCA = 272 reads: +418 validated
umi TACCCACAGC = 173 reads: +418 validated
umi TTTCAATTGC = 170 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=4)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQCGSFPRTF at 368, score = 9 + 7
umis assigned: [33, 216]
of which 2 are surviving nonsolos
reads assigned: 467
start codons at 44, 252, 471
confident = true

TIG 2[bases=568]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=9)
449-497 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
497-568 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 4 umis using 88 reads
cdr3 = CAKDRLPTRTAFDYW at 421, score = 9 + 7
umis assigned: [29, 121, 139, 276, 348]
of which 5 are surviving nonsolos
reads assigned: 933
start codons at 79, 230, 235, 382
confident = true
now this is a cell
paired!

GCCGAGGACACGGCCATATATTACTGTGCGAAAGATCGTCTACCAACTCGTACCGCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTGTGGTAGCTTTCCTAGAACGTTCGGCCAAGGGACCAAGGTGGAACTCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.120 = TGCGTGGTCGGGAGTA-1

using 379 reads

====================================================================================

graph has 530 edges initially, 6 edges after simplification

total ucounts = 188
nonsolo ucounts = 72[2^27, 3^17, 4^14, 5^3, 6^6, 7^2, 9^2, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.132 = TGCGTGGTCTTAACCT-1

using 388 reads

====================================================================================

graph has 522 edges initially, 8 edges after simplification

total ucounts = 216
nonsolo ucounts = 77[2^33, 3^30, 4^2, 5^3, 6^2, 7^5, 8, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.140 = TGCTACCAGAGGTAGA-1

using 279 reads

====================================================================================

graph has 196 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 271]
surviving nonsolo ucounts = 1[271]
ids = [3]

====================================================================================

UMI info for barcode TGCTACCAGAGGTAGA-1 contig 1 = GGAGCCCCAG...
umi CCTTTAGGGT = 248 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=518]
0-68 ==> 168-236 on |168|IGHV3-74|5'UTR| [len=236] (mis=0)
68-399 ==> 0-331 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=17)
440-477 ==> 26-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
477-518 ==> 0-41 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CTNLYHFGSEVW at 410, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 68, 224, 285
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.143 = TGCTACCAGATATGCA-1

using 300 reads

====================================================================================

graph has 89 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[300]
surviving nonsolo ucounts = 1[300]
ids = [0]

====================================================================================

UMI info for barcode TGCTACCAGATATGCA-1 contig 1 = GCCTGGGCCT...
umi CCTCTACCGA = 287 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=530]
0-39 ==> 13-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
39-373 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=18)
377-415 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
415-530 ==> 0-115 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 71 reads
cdr3 = CQAWDSTEVVF at 354, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 39, 44, 333, 337
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.152 = TGCTACCAGCGAAGGG-1

using 272 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[272]
surviving nonsolo ucounts = 1[272]
ids = [0]

====================================================================================

UMI info for barcode TGCTACCAGCGAAGGG-1 contig 1 = ACCCAAAAAC...
umi AGGAGCGACG = 258 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=509]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=23)
412-463 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
463-509 ==> 0-46 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CARGSTSWYDYW at 396, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 256
start codons at 54, 205, 252, 257, 289, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.154 = TGCTACCAGCGTTTAC-1

using 591 reads

====================================================================================

graph has 238 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^3, 580]
surviving nonsolo ucounts = 1[580]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=359]
4-186 ==> 163-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=5)
185-223 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
223-359 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNNWPPTF at 162, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 574
start codons at 46, 265
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.155 = TGCTACCAGCTGAACG-1

using 656 reads

====================================================================================

graph has 284 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^3, 6, 121, 518]
surviving nonsolo ucounts = 2[121, 518]
ids = [6, 10]

====================================================================================

UMI info for barcode TGCTACCAGCTGAACG-1 contig 1 = ACTTTCTGAG...
umi CGGCGATTAC = 111 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=492]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=16)
411-459 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
459-492 ==> 0-33 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CARGVSDGYHTASFFDTW at 374, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 111
start codons at 35, 79, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.160 = TGCTACCAGGGTTCCC-1

using 427 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^3, 4, 127, 283]
surviving nonsolo ucounts = 2[127, 283]
ids = [8, 11]

====================================================================================

UMI info for barcode TGCTACCAGGGTTCCC-1 contig 1 = GAATCAGTCC...
umi TCTCTCATCG = 264 reads: +388 validated

UMI info for barcode TGCTACCAGGGTTCCC-1 contig 2 = ATTCAGGTTC...
umi GATATACCAT = 108 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=465]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-465 ==> 0-52 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 261
start codons at 25, 31, 100, 236, 455
confident = false

TIG 2[bases=501]
0-62 ==> 83-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=1)
62-412 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=7)
436-489 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=0)
junction support: 1 umis using 6 reads
cdr3 = CARSYGSGNFYNYWYFDLW at 401, score = 9 + 7
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 106
start codons at 18, 62, 106, 414
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.163 = TGCTACCAGGTGCTAG-1

using 525 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[5^2, 513]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=464]
0-132 ==> 2175-2307 on rc of segment after IGKJ1 exon 1 [len=6000] (mis=0)
128-290 ==> 5838-6000 on rc of segment after IGKJ1 exon 1 [len=6000] (mis=1)
290-328 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
328-464 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [2]
of which 0 are surviving nonsolos
reads assigned: 506
start codons at 45, 98, 153, 158, 271, 370
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.167 = TGCTACCAGTATTGGA-1

using 235 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 1[226]
surviving nonsolo ucounts = 1[226]
ids = [4]

====================================================================================

UMI info for barcode TGCTACCAGTATTGGA-1 contig 1 = ACAAGAGGCA...
umi GGAATGCATA = 218 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=544]
0-31 ==> 20-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
31-387 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
387-425 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
425-544 ==> 0-119 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQSYDSSLSGLYVF at 355, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 218
start codons at 31, 185, 188, 239, 338, 365, 389
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 101.174 = TGCTACCAGTGTTGAA-1

using 1075 reads

====================================================================================

graph has 262 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[7, 256, 807]
surviving nonsolo ucounts = 2[256, 807]
ids = [7, 6]

====================================================================================

UMI info for barcode TGCTACCAGTGTTGAA-1 contig 1 = AGGAATCAGT...
umi TCGTTTTGGC = 1 reads: -388 non-validated
umi TCTTTCTTAC = 256 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [6, 7]
of which 2 are surviving nonsolos
reads assigned: 255
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!
sorting bam, mem = 0.04
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk101-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk101-ucc6a7a72ce/files/contig_bam.bam

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3.155 seconds used processing barcodes, peak mem = 0.23
