[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.35 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk097-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk097-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk097.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.0 = TCTATTGCACGTGAGA-1

using 368 reads

====================================================================================

graph has 284 edges initially, 2 edges after simplification

total ucounts = 77
nonsolo ucounts = 22[2^11, 3^2, 4^3, 5^4, 8, 245]
surviving nonsolo ucounts = 1[245]
ids = [6]

====================================================================================

UMI info for barcode TCTATTGCACGTGAGA-1 contig 1 = GAGAGCATCA...
umi AGATCTTTTC = 234 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=536]
0-64 ==> 0-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
64-417 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=1)
437-488 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=3)
488-536 ==> 0-48 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CARGGAVAGTISWFDPW at 406, score = 8 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 228
start codons at 64, 220, 262, 267, 299, 328, 361, 506
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.6 = TCTATTGCAGACTCGC-1

using 13 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.8 = TCTATTGCAGATAATG-1

using 345 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[345]
surviving nonsolo ucounts = 1[345]
ids = [0]

====================================================================================

UMI info for barcode TCTATTGCAGATAATG-1 contig 1 = GGAGGAACTG...
umi ACCAGTCTGC = 346 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=552]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=17)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 61 reads
cdr3 = CLQYSDWPRTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 342
start codons at 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.11 = TCTATTGCAGCCAGAA-1

using 1173 reads

====================================================================================

graph has 1458 edges initially, 10 edges after simplification

total ucounts = 361
nonsolo ucounts = 178[2^65, 3^30, 4^21, 5^19, 6^14, 7^8, 8^5, 9^4, 10^3, 11^5, 12, 14, 18, 246]
surviving nonsolo ucounts = 1[246]
ids = [286]

====================================================================================

UMI info for barcode TCTATTGCAGCCAGAA-1 contig 1 = GTCCCACTCA...
umi TCGTTCGGAT = 219 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=493]
0-19 ==> 8-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
19-370 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
369-407 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
407-493 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=1)
junction support: 1 umis using 54 reads
cdr3 = CLQYSSSPWTF at 346, score = 9 + 8
umis assigned: [286]
of which 1 are surviving nonsolos
reads assigned: 217
start codons at 19, 25, 94, 230, 449
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.13 = TCTATTGCAGCTGCTG-1

using 94 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 89]
surviving nonsolo ucounts = 1[89]
ids = [0]

====================================================================================

UMI info for barcode TCTATTGCAGCTGCTG-1 contig 1 = GAGTCAGTCT...
umi AGCATGACCA = 71 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=465]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
413-465 ==> 0-52 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CQQSYRRPITF at 352, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 69
start codons at 25, 31, 87, 100, 236, 335, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.14 = TCTATTGCAGGGAGAG-1

using 275 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[274]
surviving nonsolo ucounts = 1[274]
ids = [1]

====================================================================================

UMI info for barcode TCTATTGCAGGGAGAG-1 contig 1 = GATCAGGACT...
umi AGGTAAGAGA = 262 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=522]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
427-522 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CMQALQTPCSF at 366, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 257
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.15 = TCTATTGCAGTCACTA-1

using 230 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[230]
surviving nonsolo ucounts = 1[230]
ids = [0]

====================================================================================

UMI info for barcode TCTATTGCAGTCACTA-1 contig 1 = TGAGCGCAGA...
umi TGCGCGGCAC = 232 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=529]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=13)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
424-529 ==> 0-105 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CGAWDTSLSAWVF at 357, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 36, 190, 241, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.17 = TCTATTGCAGTGAGTG-1

using 262 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 6, 248]
surviving nonsolo ucounts = 1[248]
ids = [5]

====================================================================================

UMI info for barcode TCTATTGCAGTGAGTG-1 contig 1 = GAGGAATCAG...
umi TCTACAGACT = 241 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=460]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-460 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 28, 34, 103, 239
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.27 = TCTATTGCATTACGAC-1

using 277 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[272]
surviving nonsolo ucounts = 1[272]
ids = [3]

====================================================================================

UMI info for barcode TCTATTGCATTACGAC-1 contig 1 = GATCAGGACT...
umi CGCTTGCCAT = 264 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=495]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-356 ==> 0-326 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=11)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
427-495 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CMNTLPGGFTF at 366, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.29 = TCTATTGGTAAACACA-1

using 752 reads

====================================================================================

graph has 314 edges initially, 12 edges after simplification

total ucounts = 15
nonsolo ucounts = 7[2, 4^2, 6, 7, 302, 419]
surviving nonsolo ucounts = 2[302, 419]
ids = [3, 0]

====================================================================================

UMI info for barcode TCTATTGGTAAACACA-1 contig 1 = GGAGAAGAGC...
umi ACCAATAGCC = 302 reads: +385 validated

UMI info for barcode TCTATTGGTAAACACA-1 contig 2 = TGGGGGATCA...
umi AAATGATTGA = 415 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=557]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQQYGSSPRTF at 360, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 36, 244, 370, 463
confident = false

TIG 2[bases=568]
35-395 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
395-432 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CMQALQTPLTF at 371, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 407
start codons at 35, 68, 104, 192, 354, 374, 474
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.42 = TCTATTGGTCCGTGAC-1

using 300 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[297]
surviving nonsolo ucounts = 1[297]
ids = [0]

====================================================================================

UMI info for barcode TCTATTGGTCCGTGAC-1 contig 1 = AGGAGTCAGA...
umi AGACCTCCGT = 281 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=25)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-483 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQGNSFPYTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 27, 33, 89, 102, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.43 = TCTATTGGTCCTCTTG-1

using 272 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 4, 260]
surviving nonsolo ucounts = 1[260]
ids = [3]

====================================================================================

UMI info for barcode TCTATTGGTCCTCTTG-1 contig 1 = GGGGAGGAAC...
umi CACAAACAGC = 250 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=501]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=5)
387-424 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
424-501 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQYNNWPPSLTF at 357, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 36, 105, 241, 244, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.47 = TCTATTGGTCTTGATG-1

using 293 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3, 14, 33, 239]
surviving nonsolo ucounts = 1[239]
ids = [6]

====================================================================================

UMI info for barcode TCTATTGGTCTTGATG-1 contig 1 = TGGGGTCACA...
umi GCTTCGTTCG = 234 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=497]
0-39 ==> 123-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
39-379 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=24)
396-424 ==> 10-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
424-497 ==> 0-73 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 43 reads
cdr3 = CFSYGTSGRTF at 363, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 232
start codons at 39, 247, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.53 = TCTATTGGTGCTAGCC-1

using 910 reads

====================================================================================

graph has 338 edges initially, 18 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[3, 7, 315, 578]
surviving nonsolo ucounts = 2[315, 578]
ids = [6, 8]

====================================================================================

UMI info for barcode TCTATTGGTGCTAGCC-1 contig 1 = AGCAAGATGG...
umi GTATGACGCT = 576 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=542]
6-369 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=18)
368-406 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
406-542 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 86 reads
cdr3 = CQQYYNSPWTF at 345, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 567
start codons at 6, 175, 328, 448
confident = false

REJECT CONTIGS

TIG 1[bases=404]
0-196 ==> 120-316 on |225|IGKV1-27|L-REGION+V-REGION| [len=351] (mis=13)
230-268 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
268-404 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQKHDTAPWTF at 207, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 145, 190, 217, 230, 310
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.55 = TCTATTGGTGGCTCCA-1

using 940 reads

====================================================================================

graph has 250 edges initially, 6 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^3, 234, 694]
surviving nonsolo ucounts = 2[234, 694]
ids = [4, 7]

====================================================================================

UMI info for barcode TCTATTGGTGGCTCCA-1 contig 1 = AGCTTCAGCT...
umi CCTACTCCGT = 235 reads: +388 validated
umi GTCCGAGGAT = 697 reads: -286X +1 -2XX +99 invalidated

GOOD CONTIGS

TIG 1[bases=645]
0-46 ==> 10-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
46-397 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=2)
396-434 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
434-645 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CAAWDDSLSGVVF at 367, score = 7 + 8
umis assigned: [4, 7]
of which 2 are surviving nonsolos
reads assigned: 921
start codons at 46, 200, 350, 375, 380
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.56 = TCTATTGGTGGGTCAA-1

using 67 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[67]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.58 = TCTATTGGTGTGCCTG-1

using 269 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^3, 4, 15, 242]
surviving nonsolo ucounts = 1[242]
ids = [1]

====================================================================================

UMI info for barcode TCTATTGGTGTGCCTG-1 contig 1 = AGGAGTCAGT...
umi AACATATCAA = 244 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=554]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYDNLPPWTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 27, 33, 89, 102, 241, 364, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.61 = TCTATTGGTGTTTGTG-1

using 256 reads

====================================================================================

graph has 92 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 4, 23, 223]
surviving nonsolo ucounts = 2[23, 223]
ids = [6, 4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.64 = TCTATTGGTTATGTGC-1

using 305 reads

====================================================================================

graph has 169 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 18[2, 3^4, 4^4, 5, 6^2, 7^2, 11, 13, 18, 197]
surviving nonsolo ucounts = 1[197]
ids = [17]

====================================================================================

REJECT CONTIGS

TIG 1[bases=466]
2-36 ==> 5966-6000 on rc of segment after IGHV4-4 exon 1 [len=6000] (mis=0)
15-386 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=0)
398-425 ==> 0-27 on |22|IGHD3-9|D-REGION| [len=27] (mis=4)
424-466 ==> 0-42 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
cdr3 = CARGQRFSYYDILTGFRFFDYW at 375, score = 9 + 7
umis assigned: [17]
of which 1 are surviving nonsolos
reads assigned: 175
start codons at 15, 36, 80, 166
confident = false
VJ delta = 8
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.72 = TCTATTGGTTTGTGTG-1

using 5250 reads

====================================================================================

graph has 3347 edges initially, 36 edges after simplification

total ucounts = 702
nonsolo ucounts = 312[2^105, 3^57, 4^52, 5^22, 6^17, 7^15, 8^9, 9, 10^3, 11^2, 12^2, 15^2, 17, 18^2, 19, 26, 30, 31, 39, 45, 54, 66, 141, 142, 164, 173, 187, 188, 213, 217, 234, 245, 257, 268, 476, 499]
surviving nonsolo ucounts = 21[8, 10, 17, 31, 39, 54, 66, 141, 142, 164, 173, 187, 188, 213, 217, 234, 245, 257, 268, 476, 499]
ids = [22, 403, 148, 136, 248, 16, 104, 168, 681, 155, ...]

====================================================================================

UMI info for barcode TCTATTGGTTTGTGTG-1 contig 1 = CTGGGCCTCA...
umi AAGGCTGATC = 264 reads: +87 -1XX +291 invalidated
umi AATATAGTGT = 214 reads: +379 validated
umi ACTGCTGCGA = 67 reads: +379 validated
umi AGCACGTAGC = 246 reads: +379 validated
umi ATCTCACCGT = 163 reads: +379 validated
umi CCGAGTTTTG = 39 reads: +10 -3 +11 -1 +354 non-validated
umi CGCCAACTTT = 187 reads: +379 validated
umi GGCCGTGAGT = 235 reads: +379 validated
umi TGAACGGTCA = 210 reads: +379 validated
umi TGAACGTTCA = 267 reads: +379 validated

UMI info for barcode TCTATTGGTTTGTGTG-1 contig 2 = AGAGAGGAGC...
umi AACCAGTATC = 50 reads: +411 -1 +1 -20 non-validated
umi AACCTATTGC = 8 reads: +101 -154 +116 -62 non-validated
umi ATAATATTTG = 31 reads: +376 -1 +3 -53 non-validated
umi ATCCAAGTCA = 16 reads: +177 -1 +17 -1 +66 -41 +56 -3 +2 -1X +7 -1X +4 -3 +3 -1 +4 -1 +2 -1 +5 -2 +1 -1 +7 -1 +1 -1 +4 -1 +2 -15 invalidated
umi ATCCGTCTCT = 184 reads: +433 validated
umi ATTCTCATCC = 141 reads: +433 validated
umi GCATTTTGCA = 11 reads: +13 -46 +2 -1 +5 -1 +74 -47 +1 -1 +8 -1 +34 -1 +10 -37 +56 -80 +15 non-validated
umi TACATCATGG = 175 reads: +433 validated
umi TATCTAAGTG = 17 reads: -432X +1 invalidated
umi TTTCCACTCC = 142 reads: +404 -1 +13 -15 non-validated

GOOD CONTIGS

TIG 1[bases=627]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-357 ==> 0-320 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=10)
378-416 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
416-627 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 10 umis using 321 reads
cdr3 = CQAWDSSSGVVF at 352, score = 6 + 8
umis assigned: [40, 45, 104, 110, 155, 248, 279, 440, 605, 606]
of which 10 are surviving nonsolos
reads assigned: 1861
start codons at 37, 42, 98, 185, 331, 335
confident = true

TIG 2[bases=687]
0-72 ==> 7-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
72-425 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=14)
457-505 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
505-687 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 3 umis using 49 reads
cdr3 = CARDSAQYYESSGYSGIDYW at 414, score = 8 + 7
umis assigned: [16, 22, 136, 148, 149, 168, 403, 515, 545, 681]
of which 9 are surviving nonsolos
reads assigned: 753
start codons at 72, 228, 289, 307, 375, 439
confident = true
now this is a cell
paired!

ATTTATTACTGTGCGAGAGATTCGGCCCAATATTATGAGAGCAGTGGTTATTCCGGAATTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGCGGGACCCAGGCTATGGATGAGGCTGACTATTACTGTCAAGCTTGGGACAGCAGCAGTGGCGTGGTCTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.88 = TCTATTGTCAGCTCTC-1

using 307 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[307]
surviving nonsolo ucounts = 1[307]
ids = [0]

====================================================================================

UMI info for barcode TCTATTGTCAGCTCTC-1 contig 1 = GTCAGACTCA...
umi TTCTCACAGT = 306 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYDSFSWTF at 350, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 23, 29, 85, 98, 234, 237, 330, 360, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.89 = TCTATTGTCAGCTGGC-1

using 224 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[220]
surviving nonsolo ucounts = 1[220]
ids = [1]

====================================================================================

UMI info for barcode TCTATTGTCAGCTGGC-1 contig 1 = AGCTTCAGCT...
umi AATAGCGGAA = 210 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=572]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-572 ==> 0-137 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 205
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.100 = TCTATTGTCCCATTAT-1

using 1642 reads

====================================================================================

graph has 1646 edges initially, 46 edges after simplification

total ucounts = 449
nonsolo ucounts = 207[2^77, 3^38, 4^40, 5^15, 6^10, 7^7, 8^8, 9^2, 10^3, 11^2, 12, 13^2, 282, 334]
surviving nonsolo ucounts = 2[282, 334]
ids = [265, 280]

====================================================================================

UMI info for barcode TCTATTGTCCCATTAT-1 contig 1 = AGGAGTCAGA...
umi GCCTCCTTCT = 337 reads: +382 validated

UMI info for barcode TCTATTGTCCCATTAT-1 contig 2 = GAGTCAGACC...
umi GACCCTTCTC = 282 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 20-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
33-378 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
387-415 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYYSYPRTF at 354, score = 9 + 8
umis assigned: [280]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 27, 33, 89, 102, 238, 457
confident = false

TIG 2[bases=546]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=4)
373-410 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
410-546 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYNSYITF at 352, score = 8 + 8
umis assigned: [265]
of which 1 are surviving nonsolos
reads assigned: 279
start codons at 25, 31, 87, 100, 332, 452
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.112 = TCTATTGTCGCGTTTC-1

using 36 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 5, 23]
surviving nonsolo ucounts = 2[5, 23]
ids = [7, 8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.113 = TCTATTGTCGGAATCT-1

using 2569 reads

====================================================================================

graph has 2062 edges initially, 28 edges after simplification

total ucounts = 524
nonsolo ucounts = 253[2^90, 3^57, 4^34, 5^25, 6^11, 7^7, 8^9, 9^7, 10^2, 11, 12, 14^2, 22, 103, 133, 195, 220, 246, 446]
surviving nonsolo ucounts = 7[22, 103, 133, 195, 220, 246, 446]
ids = [411, 136, 262, 290, 441, 452, 244]

====================================================================================

UMI info for barcode TCTATTGTCGGAATCT-1 contig 1 = CAGCTTCAGC...
umi CAATCCGGCA = 105 reads: +388 validated
umi CTTCGCTCAC = 445 reads: +388 validated
umi GAATTCGCTA = 134 reads: +388 validated
umi GCCAGTAGAG = 195 reads: +388 validated
umi TCTTCTCTGC = 222 reads: +388 validated
umi TGCTATCATA = 242 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 9-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
47-398 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=22)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 6 umis using 208 reads
cdr3 = CATWDDSLRAWVF at 368, score = 7 + 7
umis assigned: [136, 244, 262, 290, 441, 452]
of which 6 are surviving nonsolos
reads assigned: 1328
start codons at 47, 201, 351, 376, 381, 419
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.114 = TCTATTGTCGTACGGC-1

using 1062 reads

====================================================================================

graph has 1522 edges initially, 6 edges after simplification

total ucounts = 494
nonsolo ucounts = 207[2^76, 3^52, 4^34, 5^8, 6^9, 7^10, 8^9, 9^2, 10, 11^5, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.123 = TCTATTGTCTCTTATG-1

using 318 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 8[2^6, 3, 293]
surviving nonsolo ucounts = 1[293]
ids = [11]

====================================================================================

UMI info for barcode TCTATTGTCTCTTATG-1 contig 1 = GTCAGTCCCA...
umi GTGTCAGTGC = 274 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=475]
0-23 ==> 8-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
23-374 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
372-411 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
411-475 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CHQYESVPYTF at 350, score = 9 + 7
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 23, 29, 85, 98, 237, 333, 360, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.128 = TCTATTGTCTTACCTA-1

using 335 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[331]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 97.131 = TCTATTGTCTTCGGTC-1

using 107 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 3, 96]
surviving nonsolo ucounts = 1[96]
ids = [3]

====================================================================================

UMI info for barcode TCTATTGTCTTCGGTC-1 contig 1 = CTACAACAGG...
umi CGAGGTTGTT = 89 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=497]
0-27 ==> 148-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
27-390 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
389-427 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
427-497 ==> 0-70 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CQQYYSTPWTF at 366, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 88
start codons at 27, 96, 349, 469
confident = false
NOT paired!

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BARCODE 97.133 = TCTATTGTCTTGAGGT-1

using 31 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^2, 3^2, 4, 5, 6]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 97.149 = TCTCATAAGATGTGGC-1

using 7500 reads

====================================================================================

graph has 4572 edges initially, 74 edges after simplification

total ucounts = 906
nonsolo ucounts = 435[2^149, 3^78, 4^65, 5^44, 6^15, 7^15, 8^16, 9^7, 10^6, 11^2, 12^4, 13, 14^2, 15, 23^3, 28, 32, 68, 102, 115, 129, 134, 137, 154, 161, 164, 179, 181, 202, 205, 212, 218, 226, 239, 241, 245, 253, 259, 270, 274, 306, 642]
surviving nonsolo ucounts = 22[115, 129, 134, 137, 154, 164, 179, 181, 202, 205, 212, 218, 226, 239, 241, 245, 253, 259, 270, 274, 306, 642]
ids = [274, 12, 820, 168, 314, 877, 792, 694, 786, 902, ...]

====================================================================================

UMI info for barcode TCTCATAAGATGTGGC-1 contig 1 = AGCTTCAGCT...
umi AAATATAGGC = 130 reads: +388 validated
umi ACTACGCCAT = 202 reads: +388 validated
umi ACTTAGCCCG = 242 reads: +388 validated
umi CAAGTTGGCA = 116 reads: +388 validated
umi CAATTAGCCG = 248 reads: +388 validated
umi CCGCTCGTTA = 260 reads: +388 validated
umi CCTTGCTAAG = 306 reads: +388 validated
umi GGGGAAACGG = 253 reads: +388 validated
umi GTCTTCTGGC = 214 reads: +388 validated
umi TAGATTCTCT = 228 reads: +388 validated
umi TATTAACCGA = 182 reads: +388 validated
umi TGCTGTCCTA = 203 reads: +388 validated
umi TTAAATTGCA = 125 reads: +339 -49 non-validated
umi TTTAACATTT = 168 reads: +388 validated
umi TTTTGTCCAT = 204 reads: +388 validated

UMI info for barcode TCTCATAAGATGTGGC-1 contig 2 = GGAGTCTCCC...
umi AATTCCCGAT = 527 reads: -376X +1 -6XX +1 -1XX +1 -4XX +1 -5XX +1 -2XX +1 -3XX +1 -7XX +4 invalidated
umi AGTTGTCTCG = 137 reads: +415 validated
umi GCATGAGACC = 270 reads: +415 validated
umi TCGCATTCTG = 273 reads: +415 validated
umi TGGAGTACTG = 179 reads: +415 validated
umi TGTCCTAGTG = 257 reads: +218 -7XX +3 -1X +186 invalidated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=18)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
435-646 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 14 umis using 437 reads
cdr3 = CAAWDDSLNGWVF at 368, score = 8 + 8
umis assigned: [12, 113, 123, 274, 279, 349, 373, 559, 593, 670] and 5 others
of which 15 are surviving nonsolos
reads assigned: 3033
start codons at 47, 351, 376, 381, 393
confident = true

TIG 2[bases=545]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=14)
428-474 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=11)
474-545 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 5 umis using 78 reads
cdr3 = CARHLEGDIVIWEW at 401, score = 9 + 7
umis assigned: [69, 168, 516, 732, 792, 809]
of which 6 are surviving nonsolos
reads assigned: 1609
start codons at 59, 233, 257
confident = true
now this is a cell
paired!

AAGGCCTCGGACACCGCCGTGTATTACTGTGCGCGACACTTAGAAGGGGACATTGTGATTTGGGAGTGGGGCCAGGGAACCCAGCTCACCGTCTCCTCAG <==> TGGCTCCAGTCTGAGGATGACGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

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BARCODE 97.158 = TCTCATAAGCCCAGCT-1

using 18209 reads

====================================================================================

graph has 6017 edges initially, 52 edges after simplification

total ucounts = 665
nonsolo ucounts = 334[2^91, 3^60, 4^37, 5^29, 6^14, 7^18, 8^4, 9^3, 10^3, 11^2, 13^2, 14^2, 16, 19, 36, 37, 43, 47, 57, 62, 71, 75, 100, 115, 119, 132, 143, 156, 165, 173, 177^2, 184, 203, 211, 214, 215, 219, 222, 227, 229, 233^2, 237, 254, 261, 262, 263, 264, 267, 270, 271, 272, 274, 277, 280, 291, 293, 294, 295, 307, 310, 314^2, 319, 320, 331^3, 334, 344, 347, 356^2, 361, 375, 388, 389^2, 568, 829]
surviving nonsolo ucounts = 63[37, 57, 71, 75, 100, 115, 119, 132, 143, 156, 165, 173, 177^2, 184, 203, 211, 214, 215, 219, 222, 227, 229, 233^2, 237, 254, 261, 262, 263, 264, 267, 270, 271, 272, 274, 277, 280, 291, 293, 294, 295, 307, 310, 314^2, 319, 320, 331^3, 334, 344, 347, 356^2, 361, 375, 388, 389^2, 568, 829]
ids = [131, 271, 601, 9, 187, 265, 94, 484, 401, 594, ...]

====================================================================================

UMI info for barcode TCTCATAAGCCCAGCT-1 contig 1 = ATCATCCAAC...
umi AACATTCATT = 179 reads: +406 -39 non-validated
umi ACTCTATCCT = 122 reads: +386 -1 +58 non-validated
umi AGTTGAATGT = 37 reads: +334 -111 non-validated
umi ATCAAAGCCG = 240 reads: +445 validated
umi ATCAGCAGCA = 218 reads: +416 -3 +26 non-validated
umi CAAAGATACC = 224 reads: +435 -10 non-validated
umi CCGTTCAGCC = 59 reads: +289 -25 +103 -28 non-validated
umi CGAAGGTCCT = 168 reads: +444 -1 non-validated
umi CTATGGTTTA = 173 reads: +430 -1 +1 -13 non-validated
umi GAAAAATCAT = 387 reads: -26X +2 -5XX +1 -3XX +1 -2XX +1 -2XX +402 invalidated
umi GCCAGTACTG = 145 reads: +445 validated
umi GCCGAACGGC = 305 reads: +445 validated
umi TAAAGGCGTT = 128 reads: +378 -1 +2 -1 +63 non-validated
umi TGATGTTCTG = 234 reads: +445 validated
umi TGGTGTATGC = 200 reads: +445 validated
umi TGTCTCCGCT = 157 reads: -82X +1 -2X +1 -11X +1 -1XX +1 -2XX +343 invalidated

UMI info for barcode TCTCATAAGCCCAGCT-1 contig 2 = GGGGAGGAAC...
umi AAACGTTCGC = 324 reads: +376 validated
umi AAAGTTTGTT = 74 reads: +376 validated
umi AACAGATTTG = 179 reads: +376 validated
umi AACGTCACGT = 297 reads: +376 validated
umi AAGCACCTGG = 288 reads: +376 validated
umi AATTCTACTC = 217 reads: +376 validated
umi AATTCTGCAA = 344 reads: +376 validated
umi ACAACATGGA = 352 reads: +274 -1XX +101 invalidated
umi ACATGACGGA = 300 reads: +29 -1XX +1 -10XX +1 -2XX +332 invalidated
umi ACCGTACCCT = 324 reads: +376 validated
umi AGTTGTACTA = 266 reads: +376 validated
umi CAGTCGCTCT = 272 reads: +376 validated
umi CCACTGTCGC = 310 reads: +376 validated
umi CCCTGTGTAT = 305 reads: +376 validated
umi CCGAGTGCCT = 118 reads: +376 validated
umi CGAGCTCTTT = 295 reads: +376 validated
umi CGTTATTCTG = 370 reads: +376 validated
umi CTCGTTGGCT = 317 reads: +376 validated
umi CTCTCGCACC = 252 reads: +376 validated
umi CTGCTACTAG = 358 reads: +376 validated
umi CTTACTTGCC = 261 reads: +376 validated
umi CTTATGTAAG = 262 reads: +376 validated
umi CTTTTCTCCT = 260 reads: +376 validated
umi GAGAGTCGAC = 279 reads: +376 validated
umi GATATCAAGT = 348 reads: +376 validated
umi GATTGACTAT = 388 reads: +376 validated
umi GCCATGTCTT = 274 reads: +376 validated
umi GGTACCAGTA = 231 reads: +376 validated
umi GTTCGAGTTC = 277 reads: +376 validated
umi TAATGCGGAC = 320 reads: +376 validated
umi TAGGCAGCAA = 332 reads: +376 validated
umi TCAAATAAAG = 214 reads: +376 validated
umi TCACTTCAAC = 201 reads: +376 validated
umi TCCGCCTATC = 359 reads: +376 validated
umi TCCTCAATCA = 232 reads: +376 validated
umi TGCAACGAAT = 325 reads: +376 validated
umi TGCCTTTTCG = 390 reads: +376 validated
umi TGGAGGGGAT = 230 reads: +376 validated
umi TTAAAACGTT = 71 reads: +363 -1 +12 non-validated
umi TTTATCTTCA = 187 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=574]
0-58 ==> 246-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=0)
58-411 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=3)
453-503 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
503-574 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 9 umis using 149 reads
cdr3 = CARGGDNWNYYWGGATQSRAFDIW at 400, score = 9 + 8
umis assigned: [22, 94, 131, 144, 150, 189, 271, 286, 327, 366] and 6 others
of which 16 are surviving nonsolos
reads assigned: 2913
start codons at 58, 214, 256, 322, 355, 484
confident = true

TIG 2[bases=548]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=2)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 39 umis using 1790 reads
cdr3 = CQQRSNWTF at 357, score = 9 + 8
umis assigned: [2, 9, 18, 28, 32, 50, 51, 55, 64, 75] and 30 others
of which 40 are surviving nonsolos
reads assigned: 10839
start codons at 36, 241, 244, 454
confident = true

REJECT CONTIGS

TIG 1[bases=556]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
20-83 ==> 5678-5741 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
382-420 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [132, 187, 300, 562, 638, 650]
of which 6 are surviving nonsolos
reads assigned: 1769
start codons at 31, 37, 93, 106, 245, 368, 462
confident = false
did not find CDR3
now this is a cell
paired!

GCGCGGGGCGGGGATAACTGGAACTACTATTGGGGGGGGGCTACGCAGTCTCGTGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG <==> CTCACCATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC
sorting bam, mem = 0.07
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk097-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk097-ucc6a7a72ce/files/contig_bam.bam

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9.018 seconds used processing barcodes, peak mem = 0.23
