[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.25 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk093-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk093-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk093.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.0 = TATGCCCCACTGCCAG-1

using 473 reads

====================================================================================

graph has 726 edges initially, 2 edges after simplification

total ucounts = 278
nonsolo ucounts = 72[2^33, 3^13, 4^9, 5^2, 6^2, 7^8, 8^2, 9, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.4 = TATGCCCCAGACAAAT-1

using 148 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 3[2^2, 135]
surviving nonsolo ucounts = 1[135]
ids = [0]

====================================================================================

UMI info for barcode TATGCCCCAGACAAAT-1 contig 1 = AGAGCCTGCT...
umi ACCGCGTCGT = 120 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=481]
0-50 ==> 2-52 on |206|IGHV6-1|5'UTR| [len=52] (mis=0)
50-415 ==> 0-365 on |207|IGHV6-1|L-REGION+V-REGION| [len=365] (mis=0)
429-477 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
junction support: 1 umis using 23 reads
cdr3 = CARDLWEAADFDYW at 404, score = 10 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 50, 94, 291, 297
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.15 = TATGCCCCAGGGATTG-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.18 = TATGCCCCAGTCGATT-1

using 862 reads

====================================================================================

graph has 238 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[4, 851]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.25 = TATGCCCCATCCGGGT-1

using 338 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[4, 328]
surviving nonsolo ucounts = 1[328]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=589]
0-68 ==> 5924-5992 on segment before IGLV2-5 exon 1 [len=6137] (mis=4)
41-276 ==> 0-235 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=2)
276-387 ==> 236-347 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=4) [SHIFT!]
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
428-589 ==> 0-161 on |307|IGLC3|C-REGION| [len=317] (mis=0)
cdr3 = CSSYTSSSTWVF at 364, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 41, 198, 242, 249
confident = false
not full
frameshifted full length stopped transcript of length 589
VJ delta = 28
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.27 = TATGCCCCATCTACGA-1

using 188 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 8, 175]
surviving nonsolo ucounts = 1[175]
ids = [3]

====================================================================================

UMI info for barcode TATGCCCCATCTACGA-1 contig 1 = AGCTTCAGCT...
umi CTTACTTACC = 179 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=645]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-355 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-645 ==> 0-210 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CVAWDDSLYAWVF at 368, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 173
start codons at 47, 351, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.32 = TATGCCCCATTGGCGC-1

using 403 reads

====================================================================================

graph has 186 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[403]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.40 = TATGCCCGTACCGTAT-1

using 419 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[102, 310]
surviving nonsolo ucounts = 1[310]
ids = [7]

====================================================================================

UMI info for barcode TATGCCCGTACCGTAT-1 contig 1 = GATCAGGACT...
umi TTACAAGCTC = 315 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CMQALQTPLTF at 366, score = 9 + 9
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 309
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.47 = TATGCCCGTATCGCAT-1

using 282 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 279]
surviving nonsolo ucounts = 1[279]
ids = [1]

====================================================================================

UMI info for barcode TATGCCCGTATCGCAT-1 contig 1 = GGAGTCAGTC...
umi CCGGATTTTC = 255 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=489]
0-26 ==> 5-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
26-377 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=23)
392-414 ==> 16-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-489 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQYDALPPVF at 353, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 252
start codons at 26, 32, 88, 101, 363, 366, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.69 = TATGCCCGTTCAGTAC-1

using 357 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 350]
surviving nonsolo ucounts = 1[350]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=554]
0-23 ==> 8-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
0-23 ==> 6799-6822 on rc of segment before IGKV3-34 exon 2 [len=6822] (mis=0)
12-75 ==> 5678-5741 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
23-374 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 346
start codons at 23, 29, 85, 98, 237, 360, 378, 460
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.70 = TATGCCCGTTCCCGAG-1

using 89 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[3^2, 4, 5^2, 6, 8, 13, 16, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.71 = TATGCCCGTTCCCTTG-1

using 151 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[151]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.73 = TATGCCCGTTCTGAAC-1

using 39 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 6, 9, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.76 = TATGCCCGTTGGTGGA-1

using 195 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 188]
surviving nonsolo ucounts = 1[188]
ids = [3]

====================================================================================

UMI info for barcode TATGCCCGTTGGTGGA-1 contig 1 = AGAGCTGCTC...
umi TCCTATTCAC = 165 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=509]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
378-416 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
416-509 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CQLYGTSLFTF at 355, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 164
start codons at 31, 239, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.79 = TATGCCCTCAAACCAC-1

using 299 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[293]
surviving nonsolo ucounts = 1[293]
ids = [3]

====================================================================================

UMI info for barcode TATGCCCTCAAACCAC-1 contig 1 = GGGGGCTGGG...
umi GAGTCTGGGC = 286 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=559]
45-399 ==> 0-354 on |341|IGLV2-18|L-REGION+V-REGION| [len=354] (mis=6)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
433-559 ==> 0-126 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CSSYTTSTTWVF at 369, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 45, 246, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.83 = TATGCCCTCACAGGCC-1

using 619 reads

====================================================================================

graph has 293 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 4, 230, 374]
surviving nonsolo ucounts = 2[230, 374]
ids = [2, 8]

====================================================================================

UMI info for barcode TATGCCCTCACAGGCC-1 contig 1 = AAAAACCACA...
umi CCCGAATCCG = 217 reads: +436 validated

UMI info for barcode TATGCCCTCACAGGCC-1 contig 2 = GAGTCAGACC...
umi GGGGCTTGGA = 377 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=582]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-582 ==> 0-96 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 212
start codons at 50, 248, 253, 270, 314, 347, 540
confident = false

TIG 2[bases=549]
0-31 ==> 22-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=0)
31-376 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=1)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQYYSYPWTF at 352, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 371
start codons at 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.84 = TATGCCCTCACATACG-1

using 88 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 11[2^3, 3^3, 7, 8, 14, 17, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.86 = TATGCCCTCACCAGGC-1

using 158 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 5, 146]
surviving nonsolo ucounts = 1[146]
ids = [3]

====================================================================================

UMI info for barcode TATGCCCTCACCAGGC-1 contig 1 = GGACACAGCA...
umi CAAATTTATC = 118 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=455]
9-360 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
359-397 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
397-455 ==> 0-58 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CLQYSSSPWTF at 336, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 9, 15, 84, 220, 439
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.92 = TATGCCCTCACTTACT-1

using 37 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.93 = TATGCCCTCACTTATC-1

using 28 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 2[2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.94 = TATGCCCTCAGCATGT-1

using 121 reads

====================================================================================

graph has 189 edges initially, 6 edges after simplification

total ucounts = 71
nonsolo ucounts = 20[2^8, 3^6, 4^3, 6^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.95 = TATGCCCTCAGCGACC-1

using 241 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 236]
surviving nonsolo ucounts = 1[236]
ids = [2]

====================================================================================

UMI info for barcode TATGCCCTCAGCGACC-1 contig 1 = GCTCTGCTTC...
umi GTTCAGTCTT = 238 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=653]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=5)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
442-653 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQSYDSSLSGWVF at 375, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 51, 205, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.96 = TATGCCCTCAGCTCGG-1

using 207 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 199]
surviving nonsolo ucounts = 1[199]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.99 = TATGCCCTCAGTACGT-1

using 268 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^4, 3, 250]
surviving nonsolo ucounts = 1[250]
ids = [8]

====================================================================================

UMI info for barcode TATGCCCTCAGTACGT-1 contig 1 = GGGGCTCCAA...
umi GGCACTTCCA = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=564]
45-397 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=19)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
433-564 ==> 0-131 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CSAWDSSLNVWVF at 366, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 240
start codons at 45, 184, 374, 391
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.102 = TATGCCCTCATCGGAT-1

using 404 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 10[2^2, 3^2, 4^3, 6, 13, 356]
surviving nonsolo ucounts = 1[356]
ids = [13]

====================================================================================

UMI info for barcode TATGCCCTCATCGGAT-1 contig 1 = TCTGCTTCAG...
umi TGAACCGTCA = 357 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=654]
0-49 ==> 2-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
49-405 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=3)
405-443 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
443-654 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQSYDSSLSAPYVF at 373, score = 8 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 354
start codons at 49, 203, 206, 257, 356, 383, 407, 575
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.106 = TATGCCCTCCAATGGT-1

using 31 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[31]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.117 = TATGCCCTCCGAATGT-1

using 437 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 4^2, 421]
surviving nonsolo ucounts = 1[421]
ids = [1]

====================================================================================

UMI info for barcode TATGCCCTCCGAATGT-1 contig 1 = AGTCAGACCC...
umi AGAGCTAACC = 420 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=548]
0-30 ==> 23-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
30-375 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
384-412 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 73 reads
cdr3 = CQQYYSYPRTF at 351, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 416
start codons at 24, 30, 86, 99, 235, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.123 = TATGCCCTCGCCTGTT-1

using 392 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^3, 3^3, 373]
surviving nonsolo ucounts = 1[373]
ids = [8]

====================================================================================

UMI info for barcode TATGCCCTCGCCTGTT-1 contig 1 = AGAGCTGCTC...
umi TAGCGTAGCG = 337 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=511]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
384-416 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=0)
416-511 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CQQYGSSPQTF at 355, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 336
start codons at 31, 239, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.129 = TATGCCCTCTAACCGA-1

using 598 reads

====================================================================================

graph has 218 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 7[2^3, 3, 4, 249, 327]
surviving nonsolo ucounts = 2[249, 327]
ids = [1, 7]

====================================================================================

UMI info for barcode TATGCCCTCTAACCGA-1 contig 1 = ATCAGTCCCA...
umi ATTTGCCTCC = 251 reads: +388 validated
umi CGAGACTATC = 332 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 78 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [1, 7]
of which 2 are surviving nonsolos
reads assigned: 572
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.147 = TATTACCAGACCACGA-1

using 613 reads

====================================================================================

graph has 262 edges initially, 36 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 292, 314]
surviving nonsolo ucounts = 2[292, 314]
ids = [4, 0]

====================================================================================

UMI info for barcode TATTACCAGACCACGA-1 contig 1 = GAGTCAGACC...
umi AGATTCATAT = 319 reads: +388 validated

UMI info for barcode TATTACCAGACCACGA-1 contig 2 = GGGAATCAGT...
umi TGCATTACCC = 304 reads: +22 -1XX +235 -2XX +128 invalidated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=1)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CQQYNSYSRTF at 352, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 311
start codons at 25, 31, 87, 100, 332, 455
confident = false

TIG 2[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=16)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 286
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.148 = TATTACCAGACTAGGC-1

using 212 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[212]
surviving nonsolo ucounts = 1[212]
ids = [0]

====================================================================================

UMI info for barcode TATTACCAGACTAGGC-1 contig 1 = GGGGTCACAA...
umi CGAAACCACA = 204 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=479]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=24)
395-423 ==> 10-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
423-479 ==> 0-56 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 33 reads
cdr3 = CFSYGTSGRTF at 362, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 201
start codons at 38, 246, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.149 = TATTACCAGAGACTTA-1

using 13 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.153 = TATTACCAGCACAGGT-1

using 228 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 8[2^2, 3^3, 6, 7, 200]
surviving nonsolo ucounts = 1[200]
ids = [9]

====================================================================================

UMI info for barcode TATTACCAGCACAGGT-1 contig 1 = GGGGTCACAA...
umi TGGTCAGGGT = 193 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=472]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=9)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
426-472 ==> 0-46 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CCSYGGRSTLVF at 362, score = 7 + 9
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 38, 177, 246, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.156 = TATTACCAGCCCTAAT-1

using 11 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.158 = TATTACCAGCTGCAAG-1

using 2179 reads

====================================================================================

graph has 2039 edges initially, 36 edges after simplification

total ucounts = 433
nonsolo ucounts = 297[2^50, 3^33, 4^36, 5^25, 6^25, 7^19, 8^18, 9^19, 10^15, 11^8, 12^10, 13^10, 14^7, 15^7, 16^6, 17, 18^3, 19, 20, 22, 24, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.172 = TATTACCAGTACACCT-1

using 283 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2, 3^3, 5, 8^2, 244]
surviving nonsolo ucounts = 1[244]
ids = [7]

====================================================================================

UMI info for barcode TATTACCAGTACACCT-1 contig 1 = GGGTCTCAGG...
umi GCAATTCTGG = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=534]
37-398 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
387-425 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
425-534 ==> 0-109 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CSSYTSSSTLVF at 361, score = 8 + 9
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 37, 194, 238, 245, 248
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.175 = TATTACCAGTCAATAG-1

using 131 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 125]
surviving nonsolo ucounts = 1[125]
ids = [1]

====================================================================================

UMI info for barcode TATTACCAGTCAATAG-1 contig 1 = AGTCTGGGCC...
umi GAGGGAACCT = 123 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=483]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-372 ==> 0-332 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=32)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-483 ==> 0-61 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CQVWDRSRDHVVF at 355, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 119
start codons at 40, 101, 188, 239, 383
confident = false
NOT paired!

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BARCODE 93.181 = TATTACCCAAACCTAC-1

using 20 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[4, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 93.186 = TATTACCCAAGTCTGT-1

using 211 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[2^3, 4, 5, 195]
surviving nonsolo ucounts = 1[195]
ids = [2]

====================================================================================

UMI info for barcode TATTACCCAAGTCTGT-1 contig 1 = GAGAAGAGCT...
umi CCTCAGTCGA = 177 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=492]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=13)
388-426 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
426-492 ==> 0-66 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQYGSSPPEVTF at 359, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 176
start codons at 35, 369, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.188 = TATTACCCAATGTAAG-1

using 395 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[4, 5, 46, 337]
surviving nonsolo ucounts = 1[337]
ids = [6]

====================================================================================

UMI info for barcode TATTACCCAATGTAAG-1 contig 1 = ACCCAGACGG...
umi TTTGTCGGGC = 315 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
14-359 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=6)
363-402 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
402-492 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 70 reads
cdr3 = CQQYNNWPPMYTF at 335, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 14, 83, 219, 362, 444
confident = false
NOT paired!

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BARCODE 93.189 = TATTACCCACAACGCC-1

using 49 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 3, 4, 10, 11, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.196 = TATTACCCACTTAAGC-1

using 290 reads

====================================================================================

graph has 135 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[288]
surviving nonsolo ucounts = 1[288]
ids = [0]

====================================================================================

UMI info for barcode TATTACCCACTTAAGC-1 contig 1 = GAGTCAGTCT...
umi AACCTTCAAT = 265 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=505]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
413-505 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQSYRRPITF at 352, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 25, 31, 87, 100, 236, 335, 455
confident = false
NOT paired!

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BARCODE 93.208 = TATTACCCATGTAGTC-1

using 336 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 330]
surviving nonsolo ucounts = 1[330]
ids = [1]

====================================================================================

UMI info for barcode TATTACCCATGTAGTC-1 contig 1 = GAATCAGTCC...
umi TATGAGACCG = 335 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 326
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 93.220 = TATTACCGTCAAAGAT-1

using 265 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[265]
surviving nonsolo ucounts = 1[265]
ids = [0]

====================================================================================

UMI info for barcode TATTACCGTCAAAGAT-1 contig 1 = TGGGGAGGAA...
umi CCATACCATT = 252 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=471]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
382-420 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
420-471 ==> 0-51 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CLQHKSYPFTF at 359, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!
sorting bam, mem = 0.03
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk093-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk093-ucc6a7a72ce/files/contig_bam.bam

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2.750 seconds used processing barcodes, peak mem = 0.23
