[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.12 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk089-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk089-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk089.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.0 = TACCTATGTAGAGGAA-1

using 16917 reads

====================================================================================

graph has 5205 edges initially, 48 edges after simplification

total ucounts = 582
nonsolo ucounts = 312[2^92, 3^58, 4^32, 5^26, 6^16, 7^8, 8^7, 9^6, 10, 11^2, 12^2, 13^2, 14, 16, 18, 57, 63, 64, 92, 122, 127, 129, 131, 143, 157, 160, 168, 179, 185, 186^2, 190, 192, 199, 201, 207, 209, 215, 217, 218, 219^2, 223, 226, 227, 230^2, 233, 235, 239, 240, 247, 256, 262, 263, 264, 278, 284^2, 286, 298^2, 299, 302, 315, 347, 417, 423, 476, 528, 1100, 1894]
surviving nonsolo ucounts = 55[57, 92, 122, 127, 129, 131, 143, 157, 160, 168, 179, 185, 186^2, 190, 192, 199, 201, 207, 209, 215, 217, 218, 219^2, 223, 226, 227, 230^2, 233, 235, 239, 240, 247, 256, 262, 263, 264, 278, 284^2, 286, 298^2, 299, 302, 315, 347, 417, 423, 476, 528, 1100, 1894]
ids = [87, 150, 434, 577, 381, 158, 356, 23, 392, 363, ...]

====================================================================================

UMI info for barcode TACCTATGTAGAGGAA-1 contig 1 = GGGGTCACAA...
umi ACCATTATGC = 159 reads: +376 validated
umi ATACTACGGT = 322 reads: +376 validated
umi ATATTAGTCT = 3 reads: -312 +3 -1XX +1 -1XX +1 -4XX +1 -5XX +1 -5XX +2 -5XX +2 -1XX +28 -3 invalidated
umi CAACGCCTCC = 236 reads: +376 validated
umi CACGTACGAC = 348 reads: +376 validated
umi CATCGATGGC = 299 reads: +376 validated
umi CCAGATCCAC = 219 reads: +376 validated
umi CCATAAGGCT = 215 reads: +279 -3XX +2 -3XX +3 -3XX +1 -2XX +80 invalidated
umi CCCGCTCGCC = 217 reads: +376 validated
umi CCCTCCATCA = 93 reads: +376 validated
umi CCGTATACCA = 219 reads: +376 validated
umi CCTACGATGC = 188 reads: +376 validated
umi CCTGGTTATT = 224 reads: +376 validated
umi CCTTCCCTGT = 296 reads: +376 validated
umi CCTTGCCTAT = 277 reads: +376 validated
umi CGAAATCTTT = 242 reads: +376 validated
umi CGCATTGTTA = 238 reads: +376 validated
umi CGCCTTTCCT = 2 reads: -318X +1 -4XX +1 -5XX +1 -5XX +2 -5XX +2 -1XX +29 -2 invalidated
umi CTAAATGCCA = 248 reads: +376 validated
umi CTCCATTGTT = 283 reads: +376 validated
umi CTCGAATCTT = 268 reads: +376 validated
umi CTGCAAGGGG = 420 reads: -373X +3 invalidated
umi GACGGTGTGC = 1 reads: -312 +3 -1X +1 -1X +1 -4X +1 -5X +1 -5X +2 -5X +2 -1X +2 -1 +20 -8 invalidated
umi GACGTGCTTT = 201 reads: +376 validated
umi GCATATACGT = 227 reads: +79 -1XX +1 -2XX +1 -3XX +289 invalidated
umi GCGTCTATTG = 212 reads: +376 validated
umi GGCCATACCA = 230 reads: +376 validated
umi GGCCTGAAAG = 1900 reads: -299 +2 -2XX +73 invalidated
umi GTCGAGCGCT = 148 reads: +376 validated
umi GTGCTATTCT = 167 reads: +376 validated
umi GTGCTGTGTA = 286 reads: +376 validated
umi GTTTATAGTT = 231 reads: +376 validated
umi TAATTTCGGT = 137 reads: +181 -2XX +1 -2XX +190 invalidated
umi TACATTATTA = 1100 reads: -299 +2 -2XX +73 invalidated
umi TACGGCTCAT = 162 reads: +376 validated
umi TAGTCCCTTC = 298 reads: +376 validated
umi TCACGACCAG = 189 reads: +376 validated
umi TCAGGGCCTT = 120 reads: +376 validated
umi TCAGTGTAGC = 231 reads: +376 validated
umi TCAGTTACCG = 471 reads: +376 validated
umi TCCTCTAATC = 258 reads: +376 validated
umi TCTGGCTATA = 428 reads: -373X +3 invalidated
umi TCTGTTCTCC = 295 reads: +376 validated
umi TCTTAAAACT = 204 reads: +376 validated
umi TCTTCAAACA = 237 reads: +376 validated
umi TGCGCCTATT = 237 reads: +376 validated
umi TTATCTGCGG = 184 reads: +376 validated
umi TTCGTACCCT = 527 reads: +376 validated
umi TTTATATATT = 260 reads: +376 validated
umi TTTTAGGGTT = 127 reads: +376 validated

UMI info for barcode TACCTATGTAGAGGAA-1 contig 2 = GGAGTCTCCC...
umi CACCGTATCT = 171 reads: +430 validated
umi CCGTATACTG = 129 reads: +379 -1 +1 -20 +9 -1 +2 -2 +2 -2 +4 -1 +5 -1 non-validated
umi GATCCACCGT = 253 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=625]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=2)
376-414 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
414-625 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 42 umis using 1628 reads
cdr3 = CCSYAAVF at 362, score = 8 + 8
umis assigned: [23, 54, 58, 76, 94, 109, 130, 133, 146, 150] and 40 others
of which 50 are surviving nonsolos
reads assigned: 13848
start codons at 38, 177, 239, 246, 372
confident = true

TIG 2[bases=517]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=0)
438-489 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
489-517 ==> 0-28 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 2 umis using 31 reads
cdr3 = CARHKGRSAAGVYNWFDPW at 401, score = 8 + 7
umis assigned: [88, 158, 298]
of which 3 are surviving nonsolos
reads assigned: 543
start codons at 59, 233, 257, 392, 507
confident = true
now this is a cell
paired!

GCCATGTATTACTGTGCGAGACATAAGGGACGCTCAGCAGCTGGTGTGTACAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> TCCCTGACAATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGCCGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.6 = TACCTATGTCAAAGAT-1

using 340 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[340]
surviving nonsolo ucounts = 1[340]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.30 = TACCTATGTGTGGCTC-1

using 161 reads

====================================================================================

graph has 203 edges initially, 2 edges after simplification

total ucounts = 84
nonsolo ucounts = 27[2^12, 3^3, 4^4, 5, 6^3, 7^2, 8, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.42 = TACCTATGTTGATTGC-1

using 337 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 4, 324]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.43 = TACCTATGTTGGTTTG-1

using 308 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[308]
surviving nonsolo ucounts = 1[308]
ids = [0]

====================================================================================

UMI info for barcode TACCTATGTTGGTTTG-1 contig 1 = TCTGGGGAGG...
umi GCCGGATCAG = 302 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=506]
0-39 ==> 58-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
39-384 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=5)
384-421 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
421-506 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQQYNDWPLTF at 360, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 39, 108, 244, 373, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.54 = TACCTATTCATATCGG-1

using 431 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 2[2, 421]
surviving nonsolo ucounts = 1[421]
ids = [2]

====================================================================================

UMI info for barcode TACCTATTCATATCGG-1 contig 1 = GAGGAACTGC...
umi AGTGGCGCTG = 422 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 64-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
33-378 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=35)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 77 reads
cdr3 = CEQYNFWPRTF at 354, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 417
start codons at 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.55 = TACCTATTCATCATTC-1

using 334 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[5, 324]
surviving nonsolo ucounts = 1[324]
ids = [4]

====================================================================================

UMI info for barcode TACCTATTCATCATTC-1 contig 1 = AGGAATCAGA...
umi GCCTGAGGGC = 308 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=503]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=6)
383-415 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
415-503 ==> 0-88 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYNSYPPTF at 354, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 27, 33, 89, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.56 = TACCTATTCATCGATG-1

using 52 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[7, 42]
surviving nonsolo ucounts = 1[42]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=452]
0-333 ==> 4-337 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=27)
347-384 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=4)
384-452 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQMFSIPFTF at 323, score = 6 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 38
start codons at 2, 58, 71, 207, 210, 300, 332, 426
confident = false
not full
VJ delta = 13
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.71 = TACCTATTCGAGCCCA-1

using 226 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 4, 215]
surviving nonsolo ucounts = 1[215]
ids = [3]

====================================================================================

UMI info for barcode TACCTATTCGAGCCCA-1 contig 1 = GCTCTGCTTC...
umi GACCTACGTT = 212 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=584]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-584 ==> 0-139 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 211
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.74 = TACCTATTCGCGTAGC-1

using 222 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 3[2^2, 210]
surviving nonsolo ucounts = 1[210]
ids = [1]

====================================================================================

UMI info for barcode TACCTATTCGCGTAGC-1 contig 1 = AGGAGTCAGT...
umi AGGCTAGGGC = 199 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=479]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-479 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CHQYESVPYTF at 354, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 197
start codons at 27, 33, 89, 102, 241, 337, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.76 = TACCTATTCGGTTAAC-1

using 526 reads

====================================================================================

graph has 198 edges initially, 12 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^2, 4, 228, 288]
surviving nonsolo ucounts = 2[228, 288]
ids = [6, 5]

====================================================================================

UMI info for barcode TACCTATTCGGTTAAC-1 contig 1 = GTGGGCTCAG...
umi TTCGTCTGGC = 278 reads: +379 validated

UMI info for barcode TACCTATTCGGTTAAC-1 contig 2 = AGCTTCAGCT...
umi TTTTTCGGCA = 227 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=573]
0-36 ==> 4-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
36-373 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=15)
377-415 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
415-573 ==> 0-158 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CHSRDSRGPWVF at 351, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 36, 155, 235, 334
confident = false

TIG 2[bases=593]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=10)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
435-593 ==> 0-158 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CAVWDDSLNGVVF at 368, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 47, 258, 351, 376, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.83 = TACCTATTCTGCCAGG-1

using 344 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[343]
surviving nonsolo ucounts = 1[343]
ids = [0]

====================================================================================

UMI info for barcode TACCTATTCTGCCAGG-1 contig 1 = GGGGAGGAAC...
umi GCGGCACTTG = 344 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=17)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CLQYSDWPRTF at 357, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 339
start codons at 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.85 = TACCTATTCTGGCGTG-1

using 5521 reads

====================================================================================

graph has 2382 edges initially, 48 edges after simplification

total ucounts = 447
nonsolo ucounts = 188[2^60, 3^38, 4^20, 5^13, 6^20, 7^6, 9^2, 10^2, 15^2, 17, 41, 65, 77, 86, 102, 111, 112, 131, 139, 154, 172, 186, 203^2, 210, 215, 249, 261, 265, 275, 286, 303, 319, 470]
surviving nonsolo ucounts = 22[65, 77, 86, 102, 111, 112, 131, 139, 154, 172, 203^2, 210, 215, 249, 261, 265, 275, 286, 303, 319, 470]
ids = [272, 13, 15, 212, 239, 338, 67, 90, 393, 322, ...]

====================================================================================

UMI info for barcode TACCTATTCTGGCGTG-1 contig 1 = GGAGTCTCCC...
umi AGAAGCCTCA = 201 reads: +432 -1 +6 -1 +1 -1 +2 -1 +2 -1 +1 -5 non-validated
umi AGCGTAACTT = 133 reads: +434 -20 non-validated
umi ATATGTACTT = 136 reads: +431 -23 non-validated
umi CTCAAACGTG = 103 reads: +425 -29 non-validated
umi CTTAGACGTG = 281 reads: +122 -2XX +3 -3XX +1 -4XX +1 -3XX +1 -86XX +1 -2XX +2 -1XX +1 -2XX +1 -3XX +2 -1XX +1 -4XX +2 -4XX +201 invalidated
umi GACCAATGCG = 115 reads: +447 -7 non-validated
umi GCTGCGGCAT = 64 reads: +379 -1 +6 -1 +1 -66 non-validated
umi GTCCTCTAGT = 213 reads: +454 validated
umi GTTGGGGCGT = 173 reads: +454 validated
umi TACAGTGCTC = 118 reads: +122 -2XX +3 -3XX +1 -4XX +1 -3XX +1 -89X +2 -1XX +1 -2XX +1 -3XX +2 -1XX +1 -4XX +2 -4XX +201 invalidated

UMI info for barcode TACCTATTCTGGCGTG-1 contig 2 = GCTCTGCTTC...
umi AACTGTTGCC = 80 reads: +98 -1X +298 invalidated
umi AAGGCCTTGA = 471 reads: +397 validated
umi ACCCTACAGC = 214 reads: +397 validated
umi CTATCAAGCG = 322 reads: +397 validated
umi CTATCCTGCA = 247 reads: +397 validated
umi GCACCGCTTA = 81 reads: -15 +11 -1XX +6 -1XX +4 -2XX +24 -2XX +5 -1XX +5 -1XX +5 -1XX +1 -1XX +3 -1XX +3 -1XX +1 -1XX +2 -1XX +2 -1XX +3 -3XX +20 -1XX +1 -1XX +4 -4XX +2 -1XX +1 -1XX +2 -1XX +1 -4XX +1 -1XX +1 -3XX +1 -51 +1 -4XX +1 -4XX +1 -1XX +11 -1XX +8 -1XX +2 -1XX +22 -1XX +1 -1XX +1 -1XX +9 -1XX +2 -1XX +2 -1XX +12 -29X +1 -12X +1 -4XX +1 -10XX +1 -2XX +3 -1XX +29 -2XX invalidated
umi GGCGGACAAA = 274 reads: +397 validated
umi GTAATGACAG = 309 reads: +397 validated
umi TAACCTCAGC = 202 reads: +397 validated
umi TGAACTTGTC = 166 reads: +76 -1XX +320 invalidated
umi TGCTTGAGCC = 285 reads: +397 validated
umi TTACCTCATA = 264 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=673]
0-59 ==> 0-59 on |200|IGHV5-51|5'UTR| [len=59] (mis=2)
59-367 ==> 0-308 on |201|IGHV5-51|L-REGION+V-REGION| [len=351] (mis=17)
462-513 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=6)
513-673 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 3 umis using 97 reads
cdr3 = CVRLHYFHDAGSFGGPGSALSHWFDPW at 401, score = 8 + 7
umis assigned: [57, 67, 90, 212, 226, 239, 272, 309, 322, 338]
of which 10 are surviving nonsolos
reads assigned: 1491
start codons at 59, 233, 423, 426, 567
confident = true

TIG 2[bases=659]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=13)
410-448 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
448-659 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 11 umis using 398 reads
cdr3 = CQSYDTSLSGSDWVF at 375, score = 8 + 8
umis assigned: [13, 18, 43, 210, 211, 252, 283, 300, 331, 393] and 2 others
of which 11 are surviving nonsolos
reads assigned: 2853
start codons at 51, 205, 358, 385
confident = true
now this is a cell
paired!

CATTACTTTCATGATGCTGGGAGTTTTGGAGGACCCGGCTCGGCTTTGTCTCACTGGTTCGACCCCTGGGGCCCGGGAACCCAGGTCACAGTCTCCTCAG <==> CAGGGTGAGGATGAGGCTACTTATTACTGCCAGTCTTATGACACCAGCCTGAGTGGTTCCGATTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTGC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.87 = TACCTATTCTGGTGTA-1

using 539 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 531]
surviving nonsolo ucounts = 1[531]
ids = [5]

====================================================================================

UMI info for barcode TACCTATTCTGGTGTA-1 contig 1 = AGCTCTGGGA...
umi TTGTATTTCT = 523 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=518]
79-432 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=2)
455-518 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=5)
junction support: 1 umis using 33 reads
cdr3 = CARESYDFWTLRDHNYYGMDVW at 421, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 514
start codons at 79, 230, 235, 293, 296, 382, 475
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.92 = TACCTATTCTTCGGTC-1

using 262 reads

====================================================================================

graph has 104 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^3, 15, 236]
surviving nonsolo ucounts = 1[236]
ids = [4]

====================================================================================

UMI info for barcode TACCTATTCTTCGGTC-1 contig 1 = GAGAAGAGCT...
umi GAATGTCGAC = 229 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=505]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=4)
420-505 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQYGSSPTTF at 359, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 222
start codons at 35, 243, 369, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.102 = TACCTTAAGAGTCTGG-1

using 310 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[67, 238]
surviving nonsolo ucounts = 2[67, 238]
ids = [2, 1]

====================================================================================

UMI info for barcode TACCTTAAGAGTCTGG-1 contig 1 = GGGCTGGGGT...
umi ACGGCGTCTG = 234 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=511]
43-404 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
393-431 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
431-511 ==> 0-80 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CSSYTSSSTLVF at 367, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 231
start codons at 43, 200, 244, 251, 254
confident = false

REJECT CONTIGS

TIG 1[bases=408]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
0-33 ==> 5967-6000 on rc of segment after IGKV1OR2-118 exon 1 [len=6000] (mis=2)
10-74 ==> 5672-5736 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=2)
12-84 ==> 8895-8967 on segment before IGKV1OR2-3 exon 1 [len=9099] (mis=8)
12-84 ==> 8904-8976 on rc of segment before IGKV2OR2-10 exon 1 [len=9109] (mis=8)
12-84 ==> 8903-8975 on segment before IGKV1OR2-11 exon 1 [len=9108] (mis=8)
27-378 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=6)
383-408 ==> 7-32 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 64
start codons at 27, 33, 89, 102, 238
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.103 = TACCTTAAGATAGTCA-1

using 213 reads

====================================================================================

graph has 414 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[213]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.105 = TACCTTAAGATGTGTA-1

using 994 reads

====================================================================================

graph has 960 edges initially, 22 edges after simplification

total ucounts = 261
nonsolo ucounts = 119[2^44, 3^28, 4^14, 5^13, 6^9, 7^4, 8, 9, 10, 11^2, 117, 311]
surviving nonsolo ucounts = 2[117, 311]
ids = [142, 132]

====================================================================================

UMI info for barcode TACCTTAAGATGTGTA-1 contig 1 = AGAGCTCTGG...
umi CTTATTTATG = 295 reads: +385 validated

UMI info for barcode TACCTTAAGATGTGTA-1 contig 2 = AGGAGTCAGA...
umi GACCCCTTAG = 106 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=522]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=2)
390-429 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
429-522 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYGSSRYTF at 368, score = 9 + 8
umis assigned: [132]
of which 1 are surviving nonsolos
reads assigned: 293
start codons at 44, 252, 378, 471
confident = false

TIG 2[bases=508]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=17)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
415-508 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CQQYNSYALSF at 354, score = 8 + 7
umis assigned: [142]
of which 1 are surviving nonsolos
reads assigned: 106
start codons at 27, 33, 89, 102, 238, 241, 334, 373, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.112 = TACCTTAAGCGTTGCC-1

using 181 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[181]
surviving nonsolo ucounts = 1[181]
ids = [0]

====================================================================================

UMI info for barcode TACCTTAAGCGTTGCC-1 contig 1 = AGCTTCAGCT...
umi GGCCTGTCCC = 181 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=553]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=1)
400-438 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
438-553 ==> 0-115 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CAAWDDSLNGHYVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 177
start codons at 47, 351, 376, 381, 393, 402
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.115 = TACCTTAAGCTTATCG-1

using 8056 reads

====================================================================================

graph has 4521 edges initially, 50 edges after simplification

total ucounts = 791
nonsolo ucounts = 406[2^157, 3^92, 4^47, 5^29, 6^20, 7^9, 8^7, 9^5, 10^2, 11^6, 13^2, 30, 39, 55, 86, 92, 118, 150, 155, 157, 180, 188, 202^2, 206, 219, 221, 235^2, 241, 244^2, 246, 247, 257, 274, 277, 284, 310, 357, 600]
surviving nonsolo ucounts = 27[39, 55, 92, 150, 155, 157, 180, 188, 202^2, 206, 219, 221, 235^2, 241, 244^2, 246, 247, 257, 274, 277, 284, 310, 357, 600]
ids = [155, 673, 594, 65, 529, 509, 419, 174, 11, 664, ...]

====================================================================================

UMI info for barcode TACCTTAAGCTTATCG-1 contig 1 = ACCCAAAAAC...
umi ATCCGCTTGG = 39 reads: -33 +388 non-validated
umi GCATTATGTC = 234 reads: +421 validated
umi GTCGGTCTCC = 154 reads: +421 validated

UMI info for barcode TACCTTAAGCTTATCG-1 contig 2 = AGGAGTCAGA...
umi AATCTGTCGC = 309 reads: +391 validated
umi AATTATGGCT = 246 reads: +391 validated
umi ACATACTCGG = 239 reads: +391 validated
umi ACCGTACCCA = 149 reads: +391 validated
umi CCCTAGTTCA = 287 reads: +391 validated
umi TATTAGAAAT = 243 reads: +391 validated
umi TCGTCCAGCC = 220 reads: +391 validated
umi TCTCCGCGCG = 204 reads: +391 validated
umi TTTATTCACT = 209 reads: +391 validated

UMI info for barcode TACCTTAAGCTTATCG-1 contig 3 = TTTATGATTT...
umi AACATCCAGC = 201 reads: +388 validated
umi ATGCTATTAC = 187 reads: +388 validated
umi CATATTATCT = 265 reads: +388 validated
umi CGTTCAAGGT = 234 reads: +388 validated
umi CTGCACACCG = 274 reads: +388 validated
umi GAGACTTTGT = 182 reads: +388 validated
umi GGGTTCATTG = 245 reads: +388 validated
umi GGTCGTCTCC = 163 reads: +388 validated
umi GTAGGGGGCT = 57 reads: -15X +2 -6XX +2 -6XX +2 -1XX +1 -1XX +1 -6XX +204 -1 +8 -1 +16 -1 +98 -16 invalidated
umi TAGCGCACAG = 92 reads: +367 -2 +19 non-validated
umi TCTGGATCCA = 59 reads: +388 validated
umi TGCACGCTAC = 357 reads: +388 validated
umi TGCCTGTATC = 242 reads: +388 validated
umi TTGTGCCCTT = 276 reads: +388 validated
umi TTTCCTCGGC = 222 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=604]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-202 ==> 0-148 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=3)
202-404 ==> 151-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=9)
427-475 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
475-604 ==> 0-129 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 2 umis using 39 reads
cdr3 = CARGFGVVHEWWYFEYW at 393, score = 8 + 7
umis assigned: [155, 446, 529]
of which 3 are surviving nonsolos
reads assigned: 423
start codons at 54, 202, 249, 254, 271, 286, 315, 348
confident = true
see deletion of 3 bases at pos 148 on |65|IGHV1-18|L-REGION+V-REGION|

TIG 2[bases=554]
0-27 ==> 154-181 on |256|IGKV1D-43|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |257|IGKV1D-43|L-REGION+V-REGION| [len=351] (mis=1)
380-418 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 9 umis using 309 reads
cdr3 = CQQYYSTPFFTF at 354, score = 9 + 8
umis assigned: [36, 39, 52, 65, 266, 611, 656, 664, 770]
of which 9 are surviving nonsolos
reads assigned: 2077
start codons at 27, 33, 89, 102, 241, 460
confident = true

TIG 3[bases=674]
75-428 ==> 0-353 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=4)
425-463 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
463-674 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 13 umis using 450 reads
cdr3 = CSSYTSSRTLVF at 399, score = 8 + 9
umis assigned: [11, 174, 233, 339, 373, 419, 502, 509, 524, 594] and 5 others
of which 14 are surviving nonsolos
reads assigned: 3006
start codons at 3, 75, 232, 276, 283, 286
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.120 = TACCTTAAGGCCCTCA-1

using 300 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[296]
surviving nonsolo ucounts = 1[296]
ids = [2]

====================================================================================

UMI info for barcode TACCTTAAGGCCCTCA-1 contig 1 = GGGGATCAGT...
umi CGGTTAGGTG = 280 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=509]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-509 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 89.131 = TACCTTAAGTGTTGAA-1

using 686 reads

====================================================================================

graph has 220 edges initially, 36 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 4, 320, 353]
surviving nonsolo ucounts = 2[320, 353]
ids = [2, 1]

====================================================================================

UMI info for barcode TACCTTAAGTGTTGAA-1 contig 1 = GAAGAGCTGC...
umi ATGTTAGATA = 352 reads: +382 validated

UMI info for barcode TACCTTAAGTGTTGAA-1 contig 2 = TGGGGAGACG...
umi CCCTTTTGGA = 324 reads: +194 -1XX +187 invalidated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYGSSYTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 349
start codons at 33, 241, 367, 457
confident = false

TIG 2[bases=533]
15-360 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=12)
359-397 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=4)
397-533 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQQYNNWPRTF at 336, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 315
start codons at 15, 84, 220, 439
confident = false
NOT paired!

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BARCODE 89.135 = TACCTTAAGTTTGCGT-1

using 278 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[273]
surviving nonsolo ucounts = 1[273]
ids = [2]

====================================================================================

UMI info for barcode TACCTTAAGTTTGCGT-1 contig 1 = GGGGGCTGGG...
umi ATAAGCCCTC = 261 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=518]
45-393 ==> 0-348 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=6)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
430-518 ==> 0-88 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CSSYTSSSVVF at 369, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 261
start codons at 45, 202, 246, 256
confident = false
NOT paired!

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BARCODE 89.137 = TACCTTACAAGACACG-1

using 332 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 6, 7, 314]
surviving nonsolo ucounts = 1[314]
ids = [5]

====================================================================================

UMI info for barcode TACCTTACAAGACACG-1 contig 1 = TGATCAGGAC...
umi TTATCGCTTG = 299 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=476]
0-31 ==> 5-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=0)
31-391 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=9)
390-428 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
428-476 ==> 0-48 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CMQALQTPRTF at 367, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 31, 64, 100, 350, 370, 470
confident = false
NOT paired!

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BARCODE 89.140 = TACCTTACAATGTTGC-1

using 14 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.06
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk089-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk089-ucc6a7a72ce/files/contig_bam.bam

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7.934 seconds used processing barcodes, peak mem = 0.23
