[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 834.09 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk082-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk082-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk082.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.0 = GTCATTTTCAACCAAC-1

using 419 reads

====================================================================================

graph has 176 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 2[3, 406]
surviving nonsolo ucounts = 1[406]
ids = [8]

====================================================================================

UMI info for barcode GTCATTTTCAACCAAC-1 contig 1 = GTCAGTCTCA...
umi TACACGGTCG = 375 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=501]
0-23 ==> 24-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
23-374 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
373-411 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
411-501 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQSYRRPITF at 350, score = 8 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 369
start codons at 23, 29, 85, 98, 234, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.4 = GTCATTTTCACCACCT-1

using 474 reads

====================================================================================

graph has 126 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^3, 171, 294]
surviving nonsolo ucounts = 2[171, 294]
ids = [6, 2]

====================================================================================

UMI info for barcode GTCATTTTCACCACCT-1 contig 1 = ATCACACAAC...
umi TGTTTTTTCG = 167 reads: +418 validated

UMI info for barcode GTCATTTTCACCACCT-1 contig 2 = GATCAGGACT...
umi ATCGCTAGCT = 293 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=606]
0-57 ==> 3-60 on |68|IGHV1-24|5'UTR| [len=60] (mis=0)
57-408 ==> 0-351 on |69|IGHV1-24|L-REGION+V-REGION| [len=351] (mis=44)
427-475 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
475-606 ==> 0-131 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 18 reads
cdr3 = CATAAGINKWAFDSW at 399, score = 10 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 167
start codons at 57, 202, 213, 255, 277, 321, 354, 425
confident = false

TIG 2[bases=498]
0-30 ==> 0-30 on |274|IGKV2D-29|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=1)
391-430 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
430-498 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CMQSIQLHSYTF at 366, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 30, 63, 99, 187, 250, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.9 = GTCATTTTCAGAGGTG-1

using 72 reads

====================================================================================

graph has 62 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[2^2, 4^2, 12, 13, 15, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.11 = GTCATTTTCAGTTAGC-1

using 134 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 124]
surviving nonsolo ucounts = 1[124]
ids = [4]

====================================================================================

UMI info for barcode GTCATTTTCAGTTAGC-1 contig 1 = GCTGTGCTGT...
umi TCCGACCAGG = 118 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=469]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
381-419 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
419-469 ==> 0-50 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQSADSSGVVF at 358, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 117
start codons at 43, 104, 173, 191
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.12 = GTCATTTTCATGCAAC-1

using 304 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[298]
surviving nonsolo ucounts = 1[298]
ids = [1]

====================================================================================

UMI info for barcode GTCATTTTCATGCAAC-1 contig 1 = AGTGACTCCT...
umi CATACCTCCC = 295 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=541]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=12)
409-459 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
459-541 ==> 0-82 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CAHRLSGALVWEYVKDTFDIW at 365, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 293
start codons at 20, 64, 243, 246, 326, 335, 402, 440
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.19 = GTCATTTTCCACGTGG-1

using 82 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 80]
surviving nonsolo ucounts = 1[80]
ids = [1]

====================================================================================

UMI info for barcode GTCATTTTCCACGTGG-1 contig 1 = CTCTCGGGAC...
umi TGTGGTACGG = 79 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=497]
0-19 ==> 143-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
19-359 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=24)
376-404 ==> 10-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
404-497 ==> 0-93 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 13 reads
cdr3 = CFSYGTSGRTF at 343, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 78
start codons at 19, 227, 353
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.25 = GTCATTTTCCGCATAA-1

using 169 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[4, 7, 10, 142]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode GTCATTTTCCGCATAA-1 contig 1 = GACTTTCTGA...
umi TTTTCTGCAC = 141 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=520]
36-384 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=11)
413-463 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
463-520 ==> 0-57 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CARDLLWFGETRAFDIW at 381, score = 9 + 8
umis assigned: [9]
of which 0 are surviving nonsolos
reads assigned: 139
start codons at 36, 80, 398, 444
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.29 = GTCATTTTCGAACGGA-1

using 385 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[385]
surviving nonsolo ucounts = 1[385]
ids = [0]

====================================================================================

UMI info for barcode GTCATTTTCGAACGGA-1 contig 1 = GGTCAGTCCC...
umi TCGCTCCAGC = 371 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=513]
30-375 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=48)
380-418 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
418-513 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYENFRSSNTF at 351, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 367
start codons at 30, 86, 99, 238, 361, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.37 = GTCATTTTCGGAATCT-1

using 15 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.44 = GTCATTTTCTACCTGC-1

using 359 reads

====================================================================================

graph has 144 edges initially, 30 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 109, 243]
surviving nonsolo ucounts = 2[109, 243]
ids = [2, 6]

====================================================================================

UMI info for barcode GTCATTTTCTACCTGC-1 contig 1 = GAGGAATCAG...
umi CAGCTTTCTC = 66 reads: +17 -1XX +30 -1XX +2 -1XX +10 -1XX +73 -1XX +3 -1XX +7 -1XX +6 -1XX +2 -2XX +5 -3XX +31 -1XX +2 -1XX +3 -1XX +4 -1XX +2 -1XX +3 -1XX +3 -2XX +4 -36XX +9 -1XX +1 -1XX +2 -1XX +3 -1XX +4 -1XX +3 -1XX +20 -1XX +18 -1XX +8 -2XX +1 -1XX +1 -43X invalidated
umi TAATAGTCGG = 231 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=467]
28-364 ==> 0-336 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=10)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
416-467 ==> 0-51 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CLQYVTYPWTF at 355, score = 9 + 7
umis assigned: [2, 6]
of which 2 are surviving nonsolos
reads assigned: 287
start codons at 28, 34, 103, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.50 = GTCATTTTCTGCAAGT-1

using 489 reads

====================================================================================

graph has 512 edges initially, 6 edges after simplification

total ucounts = 150
nonsolo ucounts = 74[2^33, 3^15, 4^10, 5^3, 6^2, 7^4, 8, 9^3, 11, 26, 135]
surviving nonsolo ucounts = 2[9, 135]
ids = [47, 62]

====================================================================================

UMI info for barcode GTCATTTTCTGCAAGT-1 contig 1 = AGCTTCAGCT...
umi CACCCTGCGA = 9 reads: -40 +9 -1 +14 -1 +6 -1 +5 -1 +1 -1 +7 -1 +162 -4 +7 -1 +1 -1 +6 -1 +64 -53 non-validated
umi CCCCGACTTA = 132 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=572]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-572 ==> 0-137 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [47, 62]
of which 2 are surviving nonsolos
reads assigned: 140
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.62 = GTCCTCAAGATAGTCA-1

using 33 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.78 = GTCCTCAAGGATTCGG-1

using 112 reads

====================================================================================

graph has 128 edges initially, 8 edges after simplification

total ucounts = 24
nonsolo ucounts = 16[2^2, 3, 4^3, 5^3, 6, 7^2, 9, 10, 13, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.85 = GTCCTCAAGGTAGCTG-1

using 3479 reads

====================================================================================

graph has 2724 edges initially, 36 edges after simplification

total ucounts = 659
nonsolo ucounts = 315[2^129, 3^70, 4^41, 5^27, 6^18, 7^7, 8^5, 9^3, 11, 12^3, 15, 95, 107, 112, 151, 187, 198, 215, 219, 249, 549]
surviving nonsolo ucounts = 10[95, 107, 112, 151, 187, 198, 215, 219, 249, 549]
ids = [19, 467, 106, 483, 29, 644, 597, 78, 146, 426]

====================================================================================

UMI info for barcode GTCCTCAAGGTAGCTG-1 contig 1 = GAAGTCGGCG...
umi AACGCCCCTC = 84 reads: +433 validated
umi ACTCTCACTG = 206 reads: +433 validated
umi TAATTGTCTG = 147 reads: +433 validated

UMI info for barcode GTCCTCAAGGTAGCTG-1 contig 2 = ATCACAGGCT...
umi AAGGCGTATA = 183 reads: +379 validated
umi GTTTATTATA = 107 reads: +377 -1X +1 invalidated
umi TGGGGTGCGC = 212 reads: +379 validated
umi TTGGTTCGGC = 187 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=543]
0-53 ==> 27-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=1)
53-404 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=15)
439-486 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
486-543 ==> 0-57 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 3 umis using 55 reads
cdr3 = CAKDRGGSVNIDGPFGMDVW at 395, score = 9 + 7
umis assigned: [19, 78, 483]
of which 3 are surviving nonsolos
reads assigned: 429
start codons at 53, 204, 267, 270, 288, 443
confident = true

TIG 2[bases=614]
0-96 ==> 19-115 on |368|IGLV3-9|5'UTR| [len=115] (mis=0)
96-427 ==> 0-331 on |369|IGLV3-9|L-REGION+V-REGION| [len=346] (mis=17)
437-475 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
475-614 ==> 0-139 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 4 umis using 106 reads
cdr3 = CQVWDINTGVVF at 411, score = 7 + 8
umis assigned: [29, 467, 597, 644]
of which 4 are surviving nonsolos
reads assigned: 677
start codons at 96, 157, 394
confident = true
now this is a cell
paired!

GTGTATTACTGTGCGAAAGATCGCGGGGGGTCGGTGAATATCGACGGGCCCTTCGGTATGGACGTCTGGGGCCAGGGGACCACGGTCACCGTCTCCTCAG <==> AGCAGAGCCCAAGCCGGGGATGAGGCTGACTTTTACTGTCAGGTGTGGGACATCAACACTGGGGTGGTATTCGGCGGAGGGACCAGGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.88 = GTCCTCAAGGTGCTTT-1

using 3636 reads

====================================================================================

graph has 5126 edges initially, 56 edges after simplification

total ucounts = 1480
nonsolo ucounts = 737[2^304, 3^149, 4^114, 5^61, 6^49, 7^14, 8^9, 9^10, 10^11, 11^5, 12^3, 13^2, 15, 16, 17, 18, 21, 209]
surviving nonsolo ucounts = 1[209]
ids = [408]

====================================================================================

UMI info for barcode GTCCTCAAGGTGCTTT-1 contig 1 = AGGAATCAGT...
umi CATCGCCTTT = 210 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [408]
of which 1 are surviving nonsolos
reads assigned: 205
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.91 = GTCCTCAAGTATCGAA-1

using 1619 reads

====================================================================================

graph has 1726 edges initially, 18 edges after simplification

total ucounts = 433
nonsolo ucounts = 233[2^89, 3^46, 4^35, 5^25, 6^20, 7^5, 8^5, 9^4, 10, 85, 182, 330]
surviving nonsolo ucounts = 3[85, 182, 330]
ids = [289, 393, 67]

====================================================================================

UMI info for barcode GTCCTCAAGTATCGAA-1 contig 1 = GAGGAATCAG...
umi CAGTAGGCGA = 311 reads: +388 validated
umi TATCTCTCTC = 75 reads: +388 validated

UMI info for barcode GTCCTCAAGTATCGAA-1 contig 2 = AACCACACCC...
umi TTATCAGCCT = 173 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=508]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-508 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 61 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [67, 289]
of which 2 are surviving nonsolos
reads assigned: 382
start codons at 28, 34, 103, 239, 458
confident = true

TIG 2[bases=485]
0-47 ==> 12-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
47-400 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
449-483 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 389, score = 7 + 7
umis assigned: [393]
of which 1 are surviving nonsolos
reads assigned: 169
start codons at 47, 245, 250, 267, 311, 344
confident = true
now this is a cell
paired!

TATTATTGTGCGAGAGATAAACACTTCCGCGACCCGTATTATCATTATAGTGGCGCGCTTGACCATTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATTTTGCGACTTATTACTGTCTACAGTATTCTAGTTCCCCGTGGACGTTTGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.93 = GTCCTCAAGTGCGTGA-1

using 46 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 4^2, 7, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.94 = GTCCTCAAGTGGAGAA-1

using 145 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 11[2^2, 3, 6^2, 8, 9, 11^2, 15, 68]
surviving nonsolo ucounts = 1[68]
ids = [3]

====================================================================================

UMI info for barcode GTCCTCAAGTGGAGAA-1 contig 1 = TGTGGGTAGA...
umi CCCGACCTTA = 63 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=461]
0-38 ==> 76-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
38-391 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
426-461 ==> 0-35 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CASWDDSLRGRVF at 359, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 63
start codons at 38, 192, 342, 367, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.95 = GTCCTCAAGTGGTAGC-1

using 344 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[342]
surviving nonsolo ucounts = 1[342]
ids = [1]

====================================================================================

UMI info for barcode GTCCTCAAGTGGTAGC-1 contig 1 = AGTTAGGACC...
umi CTACCCACAG = 344 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=539]
0-21 ==> 76-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
21-366 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=9)
365-403 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
403-539 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CQQFNKWPPTF at 342, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 338
start codons at 21, 90, 445
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.97 = GTCCTCAAGTTGAGTA-1

using 278 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 273]
surviving nonsolo ucounts = 1[273]
ids = [1]

====================================================================================

UMI info for barcode GTCCTCAAGTTGAGTA-1 contig 1 = GGAGGAACTG...
umi CACTACTGAT = 270 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=555]
0-34 ==> 13-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
34-379 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=1)
380-419 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQRSNWPGYTF at 355, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 267
start codons at 34, 239, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.99 = GTCCTCACAAACTGCT-1

using 13 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.102 = GTCCTCACAAGCTGGA-1

using 165 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 159]
surviving nonsolo ucounts = 1[159]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.114 = GTCCTCACACGAAGCA-1

using 1304 reads

====================================================================================

graph has 1894 edges initially, 18 edges after simplification

total ucounts = 599
nonsolo ucounts = 258[2^97, 3^68, 4^30, 5^14, 6^19, 7^11, 8^5, 9^5, 10^6, 11, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.115 = GTCCTCACACGACGAA-1

using 276 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[4, 267]
surviving nonsolo ucounts = 1[267]
ids = [1]

====================================================================================

UMI info for barcode GTCCTCACACGACGAA-1 contig 1 = GTCAGTCCCA...
umi ACTAATTCGC = 242 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=484]
0-23 ==> 8-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
23-374 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=1)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-484 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQYDNLPLTF at 350, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 240
start codons at 23, 29, 85, 98, 237, 360, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.129 = GTCCTCACATACCATG-1

using 319 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 313]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.137 = GTCCTCACATGCGCAC-1

using 684 reads

====================================================================================

graph has 254 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[7, 187, 486]
surviving nonsolo ucounts = 2[187, 486]
ids = [6, 3]

====================================================================================

UMI info for barcode GTCCTCACATGCGCAC-1 contig 1 = ACCCAGCAAC...
umi CCAACTCCCT = 489 reads: -220X +1 -1X +190 invalidated
umi TTTTATCCGT = 186 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=649]
0-55 ==> 9-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
55-408 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=38)
416-467 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=6)
467-649 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 2 umis using 171 reads
cdr3 = CAREKRSGWFDLW at 397, score = 9 + 7
umis assigned: [3, 6]
of which 2 are surviving nonsolos
reads assigned: 663
start codons at 55, 142, 253, 258, 319
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.139 = GTCCTCACATGGTCAT-1

using 175 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 5, 9, 154]
surviving nonsolo ucounts = 1[154]
ids = [3]

====================================================================================

UMI info for barcode GTCCTCACATGGTCAT-1 contig 1 = GTGAACTGAG...
umi CAATTCGCTT = 148 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=517]
0-37 ==> 138-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
37-400 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=18)
401-440 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
440-517 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CLQYYSPLSSTF at 376, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 147
start codons at 37, 106, 359, 482
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.142 = GTCCTCACATTATCTC-1

using 218 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3^2, 7, 203]
surviving nonsolo ucounts = 1[203]
ids = [1]

====================================================================================

UMI info for barcode GTCCTCACATTATCTC-1 contig 1 = TGCTTCAGCT...
umi AACTATTCGC = 193 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=529]
0-47 ==> 4-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
47-403 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
400-438 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
438-529 ==> 0-91 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQSYDTSLSGSVF at 371, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 193
start codons at 47, 201, 204, 255, 354, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.143 = GTCCTCACATTCACTT-1

using 24 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2^4, 3, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.146 = GTCCTCACATTGAGCT-1

using 236 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2^2, 3, 4, 6^2, 8, 11, 193]
surviving nonsolo ucounts = 1[193]
ids = [4]

====================================================================================

UMI info for barcode GTCCTCACATTGAGCT-1 contig 1 = AAAAACCACA...
umi CGGCATCGCT = 185 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=528]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-528 ==> 0-42 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 182
start codons at 50, 248, 253, 270, 314, 347
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.147 = GTCCTCACATTTGCTT-1

using 123 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 117]
surviving nonsolo ucounts = 1[117]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.150 = GTCCTCAGTAAGGATT-1

using 310 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 306]
surviving nonsolo ucounts = 1[306]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCAGTAAGGATT-1 contig 1 = GTCTCTCTCA...
umi ACAACGGGTC = 304 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 8-31 on |224|IGKV1-27|5'UTR| [len=31] (mis=0)
23-374 ==> 0-351 on |225|IGKV1-27|L-REGION+V-REGION| [len=351] (mis=0)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CQKYNSAPRTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 302
start codons at 23, 29, 85, 98, 234, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.164 = GTCCTCAGTCAAACTC-1

using 99 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[98]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.171 = GTCCTCAGTCCCTACT-1

using 677 reads

====================================================================================

graph has 1082 edges initially, 54 edges after simplification

total ucounts = 329
nonsolo ucounts = 130[2^50, 3^30, 4^18, 5^11, 6^7, 7^7, 8, 9^2, 10^2, 11, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.181 = GTCCTCAGTCTTTCAT-1

using 276 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 4, 9, 256]
surviving nonsolo ucounts = 1[256]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCAGTCTTTCAT-1 contig 1 = GAGCTACCAC...
umi AATCCAGTCT = 243 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=505]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=1)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=10)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
430-505 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CHQYFGTPFTF at 369, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 30, 99, 352, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.186 = GTCCTCAGTGCTCTTC-1

using 299 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^4, 287]
surviving nonsolo ucounts = 1[287]
ids = [6]

====================================================================================

UMI info for barcode GTCCTCAGTGCTCTTC-1 contig 1 = GCTCTGCTTC...
umi GGGTCGATCA = 277 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=573]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-573 ==> 0-128 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.188 = GTCCTCAGTGTCAATC-1

using 218 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[8, 206]
surviving nonsolo ucounts = 1[206]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCAGTGTCAATC-1 contig 1 = CAGCTTCAGC...
umi AGCACGACGT = 198 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=559]
0-47 ==> 9-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
47-398 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=1)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
435-559 ==> 0-124 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CAAWDDSLSGRVF at 368, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.192 = GTCCTCAGTTAGATGA-1

using 213 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3^2, 200]
surviving nonsolo ucounts = 1[200]
ids = [6]

====================================================================================

UMI info for barcode GTCCTCAGTTAGATGA-1 contig 1 = GGTAGCTCAG...
umi CGTAATTATA = 195 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=568]
0-36 ==> 50-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
36-389 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=17)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
424-568 ==> 0-144 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQTYDSSTWVF at 363, score = 6 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 194
start codons at 36, 99, 169, 190, 241, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.194 = GTCCTCAGTTCACGGC-1

using 245 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[244]
surviving nonsolo ucounts = 1[244]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCAGTTCACGGC-1 contig 1 = GATCACATAA...
umi GACCCTCGCA = 238 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=550]
59-410 ==> 0-351 on |63|IGHV1-18|L-REGION+V-REGION| [len=351] (mis=52)
435-483 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
483-550 ==> 0-67 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARLQVDAPLAHGGDYW at 401, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 59, 257, 420, 537
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.195 = GTCCTCAGTTCAGACT-1

using 271 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[8, 259]
surviving nonsolo ucounts = 1[259]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCAGTTCAGACT-1 contig 1 = CTGGGCCTCA...
umi ACCATGCCAA = 243 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=549]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-377 ==> 0-340 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=0)
375-413 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
413-549 ==> 0-136 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQAWDSSTVVF at 352, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 37, 42, 98, 185, 331, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.207 = GTCCTCAGTTTGCATG-1

using 163 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 159]
surviving nonsolo ucounts = 1[159]
ids = [2]

====================================================================================

UMI info for barcode GTCCTCAGTTTGCATG-1 contig 1 = GGGGTCTCAG...
umi CCCCATCATT = 160 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=510]
0-38 ==> 4-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=0)
38-390 ==> 0-352 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=0)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
426-510 ==> 0-84 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CCSYAGSYTWVF at 362, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 38, 177, 195, 239, 246, 249, 345, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.219 = GTCCTCATCATCTGTT-1

using 262 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3, 4, 55, 194]
surviving nonsolo ucounts = 2[55, 194]
ids = [9, 6]

====================================================================================

UMI info for barcode GTCCTCATCATCTGTT-1 contig 1 = GCTTCAGCTG...
umi TACACTGCAG = 183 reads: +388 validated
umi TTGTTTCCCT = 57 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=495]
0-46 ==> 68-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
46-399 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
396-434 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
434-495 ==> 0-61 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 34 reads
cdr3 = CASWDDSLRGRVF at 367, score = 8 + 8
umis assigned: [6, 9]
of which 2 are surviving nonsolos
reads assigned: 237
start codons at 46, 200, 350, 375, 380
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.223 = GTCCTCATCCAAAGTC-1

using 16 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.233 = GTCCTCATCCTCGCAT-1

using 235 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 7, 221]
surviving nonsolo ucounts = 1[221]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=520]
0-84 ==> 11259-11343 on segment before IGLV3-6 exon 1 [len=11502] (mis=4)
38-377 ==> 0-339 on |365|IGLV3-27|L-REGION+V-REGION| [len=339] (mis=0)
378-416 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
416-520 ==> 0-104 on |307|IGLC3|C-REGION| [len=317] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 28, 38, 99, 168, 186, 336
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.237 = GTCCTCATCGAACTGT-1

using 290 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[6, 284]
surviving nonsolo ucounts = 1[284]
ids = [0]

====================================================================================

UMI info for barcode GTCCTCATCGAACTGT-1 contig 1 = GGGAATCAGT...
umi CCTCATCCAA = 270 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=459]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-459 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 265
start codons at 27, 33, 102, 238
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.241 = GTCCTCATCGGTCTAA-1

using 614 reads

====================================================================================

graph has 174 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3^2, 160, 206, 239]
surviving nonsolo ucounts = 3[160, 206, 239]
ids = [3, 4, 6]

====================================================================================

UMI info for barcode GTCCTCATCGGTCTAA-1 contig 1 = AGGAGTCAGA...
umi CCACTTTCAG = 153 reads: -347X +1 -1XX +1 -1XX +1 -1XX +1 -1XX +39 invalidated
umi CCACTTTCGG = 201 reads: -366 +28 non-validated
umi TGTTTATGGC = 239 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=557]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=0)
384-421 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQANSFPPGLTF at 354, score = 9 + 9
umis assigned: [3, 4, 6]
of which 3 are surviving nonsolos
reads assigned: 582
start codons at 27, 33, 89, 102, 238, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.251 = GTCCTCATCTCAAACG-1

using 460 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 3, 4, 5^2, 433]
surviving nonsolo ucounts = 1[433]
ids = [13]

====================================================================================

REJECT CONTIGS

TIG 1[bases=442]
0-245 ==> 108-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
294-328 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
328-442 ==> 0-114 on |1|IGHA1|C-REGION| [len=1058] (mis=1)
cdr3 = CARDKHFRDPYYHYSGALDHW at 234, score = 7 + 7
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 272
start codons at 90, 112, 156, 189, 382
confident = false
VJ delta = 6
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.252 = GTCCTCATCTCACATT-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.258 = GTCCTCATCTGGTATG-1

using 236 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 5, 8, 217]
surviving nonsolo ucounts = 1[217]
ids = [6]

====================================================================================

UMI info for barcode GTCCTCATCTGGTATG-1 contig 1 = AGGAGTCAGT...
umi GGTGATACCA = 215 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=548]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
374-412 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQQYDNLPTF at 354, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 212
start codons at 27, 33, 89, 102, 241, 364, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.265 = GTCCTCATCTTGCAAG-1

using 388 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[386]
surviving nonsolo ucounts = 1[386]
ids = [1]

====================================================================================

UMI info for barcode GTCCTCATCTTGCAAG-1 contig 1 = GGCTTTCTGA...
umi CAAAGGGGCG = 378 reads: +460 validated

GOOD CONTIGS

TIG 1[bases=562]
15-386 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=3)
424-475 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=4)
475-562 ==> 0-87 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 36 reads
cdr3 = CARGHFYFASGSRIPGGVWFDPW at 375, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 373
start codons at 15, 36, 80, 166
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.277 = GTCGGGTCACAACGTT-1

using 113 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 111]
surviving nonsolo ucounts = 1[111]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.284 = GTCGGGTCACGAAAGC-1

using 114 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[113]
surviving nonsolo ucounts = 1[113]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=425]
0-34 ==> 0-34 on |387|IGLV7-46|5'UTR| [len=34] (mis=0)
34-385 ==> 0-351 on |388|IGLV7-46|L-REGION+V-REGION| [len=351] (mis=5)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
cdr3 = CLLFYSGARGVF at 358, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 89
start codons at 34, 242, 341
confident = false
not full
full length stopped transcript of length 425
frameshifted full length stopped transcript of length 425
VJ delta = 17
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.290 = GTCGGGTCAGACAAGC-1

using 239 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[109, 127]
surviving nonsolo ucounts = 2[109, 127]
ids = [2, 0]

====================================================================================

UMI info for barcode GTCGGGTCAGACAAGC-1 contig 1 = AGCTTCAGCT...
umi AAGTCCTCTC = 114 reads: +388 validated
umi ATAAACTGGT = 102 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=589]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-589 ==> 0-154 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 31 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 214
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.291 = GTCGGGTCAGACACTT-1

using 21 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 14]
surviving nonsolo ucounts = 2[2, 3]
ids = [2, 0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.311 = GTCGGGTCATGGGAAC-1

using 16 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.317 = GTCGGGTGTACGACCC-1

using 124 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 10, 109]
surviving nonsolo ucounts = 1[109]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=483]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
0-27 ==> 5973-6000 on rc of segment after IGKV1-5 exon 1 [len=6000] (mis=1)
0-27 ==> 5269-5296 on rc of segment before IGKV1-13 exon 2 [len=5296] (mis=1)
0-27 ==> 782-809 on rc of segment before IGKV2-23 exon 2 [len=809] (mis=1)
0-27 ==> 9983-10010 on rc of segment before IGKV2-40 exon 1 [len=10010] (mis=1)
0-27 ==> 786-813 on segment before IGKV1D-22 exon 1 [len=813] (mis=1)
12-84 ==> 8895-8967 on segment before IGKV1OR2-3 exon 1 [len=9099] (mis=6)
12-84 ==> 8904-8976 on rc of segment before IGKV2OR2-10 exon 1 [len=9109] (mis=6)
12-84 ==> 8903-8975 on segment before IGKV1OR2-11 exon 1 [len=9108] (mis=6)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
377-404 ==> 0-27 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
416-483 ==> 0-67 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNGQSRAF at 354, score = 8 + 6
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 90
start codons at 27, 33, 102, 238, 241, 334, 367, 458
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.320 = GTCGGGTGTAGCCTAT-1

using 151 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[145]
surviving nonsolo ucounts = 1[145]
ids = [0]

====================================================================================

UMI info for barcode GTCGGGTGTAGCCTAT-1 contig 1 = GAAGAGCTGC...
umi AAATGGTTGT = 131 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=514]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=7)
385-424 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
424-514 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQQYGSSPQRTTF at 357, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 130
start codons at 33, 241, 244, 367, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.323 = GTCGGGTGTATCAGTC-1

using 282 reads

====================================================================================

graph has 146 edges initially, 8 edges after simplification

total ucounts = 29
nonsolo ucounts = 11[2^4, 3, 4^2, 6, 7, 103, 129]
surviving nonsolo ucounts = 2[103, 129]
ids = [11, 8]

====================================================================================

UMI info for barcode GTCGGGTGTATCAGTC-1 contig 1 = AGCTTCAGCT...
umi GAAACCTTCT = 101 reads: +388 validated

UMI info for barcode GTCGGGTGTATCAGTC-1 contig 2 = CATGGACATG...
umi CACACTAGGC = 123 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-544 ==> 0-109 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 100
start codons at 47, 201, 351, 376, 381
confident = false

TIG 2[bases=496]
1-352 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=7)
352-389 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
435-496 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CQQYDNIVFTF at 328, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 122
start codons at 1, 7, 63, 76, 215, 338, 477
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 82.328 = GTCGGGTGTCGGCATC-1

using 151 reads

====================================================================================

graph has 128 edges initially, 16 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[43, 105]
surviving nonsolo ucounts = 2[43, 105]
ids = [1, 3]

====================================================================================
NOT paired!
sorting bam, mem = 0.04
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk082-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk082-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

4.723 seconds used processing barcodes, peak mem = 0.23
