[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.93 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk071-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk071-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk071.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.12 = GCTCTGTAGTCGTACT-1

using 970 reads

====================================================================================

graph has 885 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[969]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.20 = GCTCTGTAGTTGCAGG-1

using 182 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 176]
surviving nonsolo ucounts = 1[176]
ids = [1]

====================================================================================

UMI info for barcode GCTCTGTAGTTGCAGG-1 contig 1 = GGGGGATCTC...
umi GAATCATGCC = 172 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=484]
40-378 ==> 0-338 on |341|IGLV2-18|L-REGION+V-REGION| [len=354] (mis=16)
393-431 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=5)
431-484 ==> 0-53 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CSSYTNSTTPGVF at 364, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 171
start codons at 40, 176, 248
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.26 = GCTCTGTCAAGACACG-1

using 8312 reads

====================================================================================

graph has 4648 edges initially, 48 edges after simplification

total ucounts = 1097
nonsolo ucounts = 513[2^213, 3^87, 4^71, 5^44, 6^20, 7^27, 8^11, 9^3, 10^2, 11^5, 12^2, 14^2, 15^2, 22, 100, 133, 156, 192, 197, 214, 215, 217, 241, 265, 273, 274, 275, 286, 287, 291, 301, 314, 321, 323, 332, 351, 376]
surviving nonsolo ucounts = 22[100, 133, 156, 192, 197, 215, 217, 241, 265, 273, 274, 275, 286, 287, 291, 301, 314, 321, 323, 332, 351, 376]
ids = [540, 228, 521, 656, 90, 1011, 38, 696, 739, 751, ...]

====================================================================================

UMI info for barcode GCTCTGTCAAGACACG-1 contig 1 = AGGAGTCAGA...
umi AACTTGCGCT = 216 reads: +388 validated
umi AATTCCGAGC = 375 reads: +388 validated
umi ACACGTCGCT = 199 reads: +388 validated
umi ATATAAGGGT = 133 reads: +388 validated
umi CACACAGTAC = 273 reads: +388 validated
umi CCATTCTTCC = 321 reads: +388 validated
umi CGCTACCGGT = 283 reads: +388 validated
umi CGTCCCTCTG = 274 reads: +388 validated
umi CTATATCACC = 293 reads: +388 validated
umi CTATCTGGAG = 155 reads: +388 validated
umi CTCGTTCCTG = 100 reads: +388 validated
umi GCCGAGACCC = 313 reads: +388 validated
umi GCGAGAGGGC = 194 reads: +388 validated
umi GCTCTCCGCT = 290 reads: +388 validated
umi GGGGCTTTTG = 243 reads: +388 validated
umi GTCCCAGGCT = 264 reads: +388 validated
umi GTGATACCTT = 271 reads: +388 validated
umi TTAGTTTCCA = 216 reads: +388 validated
umi TTATCATCAT = 305 reads: +388 validated
umi TTTACTCATC = 328 reads: +388 validated
umi TTTAGCCTCA = 319 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-367 ==> 0-340 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=12)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 21 umis using 875 reads
cdr3 = CQQYSSNQYSF at 354, score = 8 + 7
umis assigned: [38, 76, 90, 228, 317, 407, 475, 493, 519, 521] and 11 others
of which 21 are surviving nonsolos
reads assigned: 5286
start codons at 27, 33, 89, 102, 189, 259, 334, 457
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.33 = GCTCTGTCAAGTAGTA-1

using 607 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[4, 596]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.53 = GCTCTGTCACCTCGTT-1

using 265 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[261]
surviving nonsolo ucounts = 1[261]
ids = [2]

====================================================================================

UMI info for barcode GCTCTGTCACCTCGTT-1 contig 1 = GAAGAGCTGC...
umi CGGGCATGAG = 245 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=487]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
418-487 ==> 0-69 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQYGSSPVTF at 357, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 33, 241, 244, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.54 = GCTCTGTCACGAAGCA-1

using 1619 reads

====================================================================================

graph has 2123 edges initially, 28 edges after simplification

total ucounts = 785
nonsolo ucounts = 340[2^154, 3^72, 4^43, 5^25, 6^19, 7^8, 8^7, 9^4, 10^3, 11, 12^2, 13^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.55 = GCTCTGTCACGAGGTA-1

using 499 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 8, 482]
surviving nonsolo ucounts = 1[482]
ids = [8]

====================================================================================

UMI info for barcode GCTCTGTCACGAGGTA-1 contig 1 = GAGTCAGTCC...
umi TGGTTGGCTG = 484 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=546]
0-25 ==> 6-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
25-376 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
372-410 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
410-546 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 76 reads
cdr3 = CQQYDNLPTF at 352, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 477
start codons at 25, 31, 87, 100, 239, 362, 452
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.61 = GCTCTGTCAGACTCGC-1

using 449 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[3, 5, 6, 434]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.78 = GCTCTGTCAGGTCGTC-1

using 274 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[5, 263]
surviving nonsolo ucounts = 1[263]
ids = [4]

====================================================================================

UMI info for barcode GCTCTGTCAGGTCGTC-1 contig 1 = AGAGAGGAGC...
umi GGATCATTCA = 239 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=503]
0-72 ==> 7-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
72-425 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=15)
430-478 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
478-503 ==> 0-25 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CARRSREVDYW at 414, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 231
start codons at 72, 228, 289, 307, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.87 = GCTCTGTCATACTCTT-1

using 334 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 4^2, 9, 315]
surviving nonsolo ucounts = 1[315]
ids = [4]

====================================================================================

UMI info for barcode GCTCTGTCATACTCTT-1 contig 1 = GTCAGTCTCA...
umi TGATCAGGGT = 306 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=507]
0-23 ==> 24-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
23-358 ==> 0-335 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=10)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
411-507 ==> 0-96 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQIFSNLSTF at 350, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 299
start codons at 23, 29, 85, 98, 234, 255, 404, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.88 = GCTCTGTCATCACCCT-1

using 23 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.90 = GCTCTGTCATCCCACT-1

using 115 reads

====================================================================================

graph has 101 edges initially, 6 edges after simplification

total ucounts = 27
nonsolo ucounts = 18[2^5, 3^3, 4, 5, 6, 7, 8, 10^3, 12, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.91 = GCTCTGTCATCCTAGA-1

using 121 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[10, 111]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.99 = GCTCTGTCATTCACTT-1

using 11645 reads

====================================================================================

graph has 12325 edges initially, 258 edges after simplification

total ucounts = 2216
nonsolo ucounts = 1658[2^301, 3^220, 4^179, 5^166, 6^126, 7^88, 8^92, 9^89, 10^68, 11^66, 12^53, 13^51, 14^39, 15^29, 16^31, 17^16, 18^11, 19^11, 20^5, 21^2, 22^6, 23^5, 25, 26, 28, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.107 = GCTCTGTGTAACGCGA-1

using 1133 reads

====================================================================================

graph has 1536 edges initially, 28 edges after simplification

total ucounts = 546
nonsolo ucounts = 224[2^89, 3^59, 4^33, 5^9, 6^13, 7^4, 8^5, 9^6, 10, 12^2, 13^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.113 = GCTCTGTGTACCGAGA-1

using 266 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3, 4, 5, 6, 8, 231]
surviving nonsolo ucounts = 1[231]
ids = [1]

====================================================================================

UMI info for barcode GCTCTGTGTACCGAGA-1 contig 1 = AGCTCTCAGA...
umi ACACCTCAGG = 227 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=532]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=15)
437-485 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
485-532 ==> 0-47 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARVGPLFDYW at 421, score = 9 + 6
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 79, 235, 356, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.116 = GCTCTGTGTACGCACC-1

using 15234 reads

====================================================================================

graph has 7981 edges initially, 100 edges after simplification

total ucounts = 1597
nonsolo ucounts = 857[2^327, 3^178, 4^103, 5^76, 6^42, 7^26, 8^14, 9^11, 10^9, 11^5, 12^2, 13^2, 14, 15, 16^2, 17, 19, 21, 22, 33, 35, 45, 49, 52, 56, 64, 70, 72, 85, 101, 115^2, 125, 127, 129, 137, 141, 145, 148, 149, 156, 166, 170, 188, 197, 199, 206, 208, 216, 221, 233, 236, 243, 244, 253, 257, 259, 261, 263, 278, 286, 289, 296, 301, 308, 330, 332, 341, 362, 366, 378, 740, 758]
surviving nonsolo ucounts = 48[49, 56, 64, 70, 85, 115^2, 125, 127, 129, 137, 141, 145, 148, 149, 156, 166, 170, 188, 197, 199, 206, 208, 216, 221, 233, 236, 243, 244, 253, 257, 259, 261, 263, 278, 286, 289, 296, 301, 308, 330, 332, 341, 362, 366, 378, 740, 758]
ids = [789, 1441, 1241, 392, 1343, 89, 1365, 537, 899, 1521, ...]

====================================================================================

UMI info for barcode GCTCTGTGTACGCACC-1 contig 1 = AGTCTGGGCC...
umi AAAAAGCGTA = 251 reads: +382 validated
umi AATCGGGCCT = 105 reads: +382 validated
umi ACAGAGGCGT = 359 reads: +382 validated
umi ACGGGCTTAG = 308 reads: +382 validated
umi AGAGGATACG = 230 reads: +382 validated
umi AGGTCTTATG = 245 reads: +382 validated
umi AGTGTTTCGG = 205 reads: +382 validated
umi CAGGTACTGA = 155 reads: +382 validated
umi CGAGGGACCG = 255 reads: +382 validated
umi CGCGGCGGCT = 126 reads: +382 validated
umi CTGCGTGACA = 263 reads: +382 validated
umi GAGGGCCTAG = 141 reads: +382 validated
umi GCCTGTCACC = 266 reads: +382 validated
umi GCGGCGTCCC = 46 reads: +382 validated
umi GGTAGACCAG = 225 reads: +382 validated
umi GGTCAGCCGT = 117 reads: +382 validated
umi GTCACCACCT = 136 reads: +382 validated
umi GTCGCAAGTA = 244 reads: +382 validated
umi TACAGGTGTT = 175 reads: +382 validated
umi TCGTCTTCGT = 201 reads: +382 validated
umi TGCTTAAACG = 281 reads: +382 validated
umi TTGAGTCTTT = 175 reads: +382 validated

UMI info for barcode GCTCTGTGTACGCACC-1 contig 2 = GGGACTCAAC...
umi AAACCGACCA = 139 reads: +397 -1 +1 -13 non-validated
umi ACTCGAGGGG = 216 reads: +412 validated
umi AGGTGCTCTT = 237 reads: +412 validated
umi ATTATGCGTC = 69 reads: +399 -1 +4 -1 +4 -2 +1 non-validated
umi CAGCTGTGTC = 191 reads: +358 -1 +53 non-validated
umi CGAGTCCTGG = 246 reads: +412 validated
umi CGGCGCCGCG = 311 reads: +412 validated
umi CGGGTCGATG = 262 reads: +316 -3XX +3 -1XX +2 -1XX +2 -1XX +2 -5XX +1 -2XX +1 -16X +4 -5XX +2 -4XX +41 invalidated
umi CGGGTGTTAG = 130 reads: +412 validated
umi CGGTTCGACT = 133 reads: +412 validated
umi CTTGGTCGCG = 292 reads: +412 validated
umi GAGAAGGACA = 341 reads: +412 validated
umi GTAGTCTCCG = 756 reads: +192 -14XX +1 -4XX +1 -200XX invalidated
umi GTCAAAACCG = 378 reads: +412 validated
umi GTTCTCAGGG = 284 reads: +412 validated
umi TACTCGTGTA = 111 reads: +412 validated
umi TAGCTAACGT = 284 reads: +412 validated
umi TATCGGAGCA = 297 reads: +412 validated
umi TCATGAGTCA = 65 reads: +307 -57 +1 -3 +6 -1 +3 -2 +1 -3 +2 -1 +1 -1 +1 -2 +3 -1 +4 -2 +10 non-validated
umi TGCAGCACGT = 85 reads: +397 -15 non-validated
umi TGCGTTTTGA = 110 reads: +391 -21 non-validated
umi TGGCGTTATC = 363 reads: +412 validated
umi TTAATATGTG = 48 reads: +276 -73 +63 non-validated
umi TTATGTTGGG = 208 reads: +412 validated
umi TTGCGTCGCT = 130 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=633]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-382 ==> 0-342 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=1)
384-422 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
422-633 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 22 umis using 696 reads
cdr3 = CQVWDSSSDFWVF at 355, score = 8 + 8
umis assigned: [0, 89, 124, 169, 220, 266, 290, 453, 518, 537] and 12 others
of which 22 are surviving nonsolos
reads assigned: 4441
start codons at 40, 101, 239, 242, 338
confident = true

TIG 2[bases=541]
58-411 ==> 0-353 on |736|IGHV1-8|L-REGION+V-REGION| [len=353] (mis=0)
422-470 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
470-541 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 17 umis using 320 reads
cdr3 = CARGFGIRPFDYW at 400, score = 9 + 7
umis assigned: [3, 184, 271, 392, 444, 520, 554, 557, 560, 566] and 15 others
of which 25 are surviving nonsolos
reads assigned: 5594
start codons at 58, 209, 256, 261, 265, 293, 322, 355
confident = true
now this is a cell
paired!

CTGAGATCTGAGGACACGGCCGTGTATTACTGTGCGAGAGGCTTTGGAATACGGCCCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGGGTCGAAGCCGGGGATGAGGCCGACTATTACTGTCAGGTGTGGGATAGTAGTAGTGATTTTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.118 = GCTCTGTGTACTCTCC-1

using 16 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.119 = GCTCTGTGTACTTCTT-1

using 32 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.151 = GCTCTGTGTCTGCGGT-1

using 341 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3, 5, 8, 9, 309]
surviving nonsolo ucounts = 1[309]
ids = [9]

====================================================================================

UMI info for barcode GCTCTGTGTCTGCGGT-1 contig 1 = AGAGCTGCTC...
umi TGTCCTTCAT = 310 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=552]
0-31 ==> 21-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
31-379 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=6)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQQHATSPFTF at 355, score = 9 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 31, 239, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.160 = GCTCTGTGTGCAGTAG-1

using 444 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[3, 4, 6, 202, 228]
surviving nonsolo ucounts = 2[202, 228]
ids = [3, 4]

====================================================================================

UMI info for barcode GCTCTGTGTGCAGTAG-1 contig 1 = AGCTTCAGCT...
umi GTCATTGGTG = 200 reads: +388 validated
umi TCTTCCTAGT = 222 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=606]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-606 ==> 0-171 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 72 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [3, 4]
of which 2 are surviving nonsolos
reads assigned: 416
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.177 = GCTCTGTGTTCGGCAC-1

using 176 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[175]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.179 = GCTCTGTGTTCGTGAT-1

using 291 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[289]
surviving nonsolo ucounts = 1[289]
ids = [0]

====================================================================================

UMI info for barcode GCTCTGTGTTCGTGAT-1 contig 1 = GAGTCAGACT...
umi ATCAACATAC = 291 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYNGQSRAF at 352, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 25, 31, 100, 236, 239, 332, 365, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.180 = GCTCTGTGTTCTCATT-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.186 = GCTCTGTGTTGGTTTG-1

using 120 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[120]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.203 = GCTCTGTTCATGCATG-1

using 12 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.209 = GCTCTGTTCCACGCAG-1

using 111 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^3, 100]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.221 = GCTCTGTTCCGTAGGC-1

using 262 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 254]
surviving nonsolo ucounts = 1[254]
ids = [3]

====================================================================================

UMI info for barcode GCTCTGTTCCGTAGGC-1 contig 1 = GAGTCCCAAC...
umi CGAATACTCT = 256 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=545]
27-275 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
371-409 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
409-545 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CLHYDNRRRTF at 348, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 250
start codons at 27, 83, 96, 235, 358, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.230 = GCTCTGTTCGCCCTTA-1

using 926 reads

====================================================================================

graph has 734 edges initially, 24 edges after simplification

total ucounts = 224
nonsolo ucounts = 83[2^29, 3^21, 4^17, 5^3, 6^5, 7, 8, 9^3, 11, 228, 270]
surviving nonsolo ucounts = 2[228, 270]
ids = [46, 84]

====================================================================================

UMI info for barcode GCTCTGTTCGCCCTTA-1 contig 1 = GTCAGACCCA...
umi ATTGTAGGTA = 227 reads: +388 validated

UMI info for barcode GCTCTGTTCGCCCTTA-1 contig 2 = AGCTTCAGCT...
umi CGAACATCAA = 268 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=461]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=5)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-461 ==> 0-50 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQYDSYPLTF at 350, score = 8 + 9
umis assigned: [46]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 23, 29, 85, 98, 330, 360, 453
confident = false

TIG 2[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [84]
of which 1 are surviving nonsolos
reads assigned: 265
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.232 = GCTCTGTTCGGCGGTT-1

using 312 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[312]
surviving nonsolo ucounts = 1[312]
ids = [0]

====================================================================================

UMI info for barcode GCTCTGTTCGGCGGTT-1 contig 1 = GGGGATCTCA...
umi ACAGGCTACA = 315 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=519]
21-369 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=17)
400-448 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=0)
448-519 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CARQVYSYGPKYYFDYW at 366, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 9, 21, 65, 388
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.239 = GCTCTGTTCTACGAGT-1

using 883 reads

====================================================================================

graph has 326 edges initially, 4 edges after simplification

total ucounts = 76
nonsolo ucounts = 22[2^9, 3^5, 4^3, 5, 6, 8^2, 757]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.256 = GCTCTGTTCTTGGGTA-1

using 50 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.274 = GCTGCAGAGATGTTAG-1

using 314 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[309]
surviving nonsolo ucounts = 1[309]
ids = [4]

====================================================================================

UMI info for barcode GCTGCAGAGATGTTAG-1 contig 1 = GCTCTGCTTC...
umi TGCATAACGA = 296 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=534]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-534 ==> 0-89 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.279 = GCTGCAGAGCGGCTTC-1

using 245 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[245]
surviving nonsolo ucounts = 1[245]
ids = [0]

====================================================================================

UMI info for barcode GCTGCAGAGCGGCTTC-1 contig 1 = GGGAGTCTCA...
umi GGTGTACTGT = 226 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=490]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=2)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
372-411 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-490 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQSYSTPCSF at 350, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 222
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.284 = GCTGCAGAGGATGCGT-1

using 189 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[186]
surviving nonsolo ucounts = 1[186]
ids = [3]

====================================================================================

UMI info for barcode GCTGCAGAGGATGCGT-1 contig 1 = AATCAGTCCC...
umi TGTTATATTC = 164 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=473]
0-24 ==> 3-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
24-375 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-473 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CLQYSSSPWTF at 351, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 24, 30, 99, 235, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.299 = GCTGCAGAGTTATCGC-1

using 341 reads

====================================================================================

graph has 190 edges initially, 30 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[43, 292]
surviving nonsolo ucounts = 2[43, 292]
ids = [7, 5]

====================================================================================

UMI info for barcode GCTGCAGAGTTATCGC-1 contig 1 = GAGTCAGACT...
umi TCTCCTTCAT = 297 reads: +388 validated
umi TGAGTTGTCC = 32 reads: +22 -1XX +25 -1XX +12 -2XX +26 -1XX +3 -1XX +16 -1X +13 -26X +4 -2XX +1 -5XX +2 -1XX +1 -1XX +5 -1XX +8 -1XX +20 -1XX +3 -1XX +3 -1XX +3 -1XX +2 -2XX +39 -2XX +27 -1XX +20 -1XX +8 -1XX +9 -61 invalidated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
376-413 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQYNSYPLTF at 352, score = 8 + 9
umis assigned: [5, 7]
of which 2 are surviving nonsolos
reads assigned: 319
start codons at 25, 31, 87, 100, 236, 239, 332, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.319 = GCTGCAGCAGGTCTCG-1

using 481 reads

====================================================================================

graph has 208 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[4, 19, 65, 192, 199]
surviving nonsolo ucounts = 4[19, 65, 192, 199]
ids = [4, 5, 3, 1]

====================================================================================

UMI info for barcode GCTGCAGCAGGTCTCG-1 contig 1 = ACTTTCTGAG...
umi CATTTAACGG = 190 reads: +412 validated

UMI info for barcode GCTGCAGCAGGTCTCG-1 contig 2 = GGGAGGAATC...
umi GGTCGCGGGA = 193 reads: +388 validated
umi GGTCGCGGGG = 20 reads: -25 +264 -1 +35 -5 +22 -1 +35 non-validated
umi GGTCGCGGGT = 64 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=565]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=8)
397-447 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
447-565 ==> 0-118 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 19 reads
cdr3 = CARDNGWVNAFDIW at 374, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 183
start codons at 35, 79, 335, 387, 399, 428
confident = true

TIG 2[bases=554]
30-381 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 41 reads
cdr3 = CLQYSSSPWTF at 357, score = 9 + 8
umis assigned: [3, 4, 5]
of which 3 are surviving nonsolos
reads assigned: 274
start codons at 30, 36, 105, 241, 460
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.321 = GCTGCAGCATATGGTC-1

using 22802 reads

====================================================================================

graph has 7329 edges initially, 58 edges after simplification

total ucounts = 1142
nonsolo ucounts = 508[2^189, 3^112, 4^61, 5^25, 6^22, 7^8, 8^4, 9^5, 10, 11, 12, 16, 17, 18, 30, 39, 61, 79, 106, 111, 112, 125, 137, 145, 148^2, 157, 173, 179, 181, 208, 226, 231, 244, 245, 246, 247, 252, 253, 254, 256, 264, 267, 269, 270^2, 271, 273, 278, 280^2, 283, 284^2, 288, 293^2, 294^2, 301, 305, 309, 312, 317, 322, 324, 326, 329, 331, 335, 341, 345^2, 353, 364, 370, 372, 375, 377^2, 378, 379, 385, 391, 398^2, 401, 410, 418, 420]
surviving nonsolo ucounts = 72[30, 79, 106, 111, 137, 145, 148^2, 157, 173, 179, 181, 208, 226, 231, 244, 245, 246, 247, 252, 253, 254, 256, 264, 267, 269, 270^2, 271, 273, 278, 280^2, 283, 284^2, 288, 293^2, 294^2, 301, 305, 309, 312, 317, 322, 324, 326, 329, 331, 335, 341, 345^2, 353, 364, 370, 372, 375, 377^2, 378, 379, 385, 391, 398^2, 401, 410, 418, 420]
ids = [6, 359, 781, 724, 727, 762, 564, 1004, 586, 67, ...]

====================================================================================

UMI info for barcode GCTGCAGCATATGGTC-1 contig 1 = GGAGAAGAGC...
umi AAACTCCACC = 379 reads: +385 validated
umi AACCTGGAGA = 332 reads: +385 validated
umi AATACTATGA = 273 reads: +385 validated
umi AATGATTCCC = 417 reads: +385 validated
umi ACACCACATC = 356 reads: +385 validated
umi ACAGGTTGCT = 283 reads: +385 validated
umi ACCATTCTAG = 385 reads: +385 validated
umi AGCACTGATC = 254 reads: +385 validated
umi ATAGGAGGGC = 287 reads: +385 validated
umi ATCGATCGGC = 272 reads: +385 validated
umi ATCTCCATGT = 377 reads: +385 validated
umi ATGCTTGGGC = 377 reads: +385 validated
umi ATTTCAGGGT = 379 reads: +385 validated
umi CAAATGTCTT = 182 reads: +385 validated
umi CAATACAAAC = 404 reads: +385 validated
umi CACGGGGTTC = 344 reads: +385 validated
umi CATAATCCTT = 307 reads: +385 validated
umi CATACATGTC = 306 reads: +385 validated
umi CATTCAACTA = 252 reads: +385 validated
umi CCGAGCTAAC = 265 reads: +385 validated
umi CCGCAGTGCT = 246 reads: +385 validated
umi CCGCCGATAC = 293 reads: +385 validated
umi CGTCGGGTTG = 323 reads: +385 validated
umi CTACAACTAA = 342 reads: +385 validated
umi CTCTCCTGTT = 273 reads: +385 validated
umi CTGCCTGTAA = 341 reads: +385 validated
umi CTGCGTCCTC = 332 reads: +385 validated
umi CTGTACCCAA = 292 reads: +385 validated
umi CTGTCTTGTA = 145 reads: +385 validated
umi CTTCGTGTCA = 256 reads: +385 validated
umi CTTTCTCAGC = 162 reads: +385 validated
umi GAACATGTAG = 263 reads: +385 validated
umi GAACCACCGG = 334 reads: +385 validated
umi GACGGCCCAA = 280 reads: +385 validated
umi GAGCCTTCGC = 236 reads: +385 validated
umi GCACTCTATA = 283 reads: +385 validated
umi GGCAGGCTCC = 367 reads: +385 validated
umi GGGAAGAAGG = 320 reads: +385 validated
umi GGGTTTCAGT = 111 reads: +385 validated
umi GGTATACATA = 135 reads: +385 validated
umi GTACAAACCA = 225 reads: +385 validated
umi GTCATTTGGG = 366 reads: +385 validated
umi GTGCGTCTCC = 107 reads: +385 validated
umi GTTAGGTTCA = 342 reads: +385 validated
umi TAGCACTTAG = 178 reads: +385 validated
umi TAGCTCAATC = 310 reads: +385 validated
umi TCATTTGCCT = 285 reads: +385 validated
umi TCCCATACAT = 242 reads: +385 validated
umi TCCGTGTTCG = 398 reads: +385 validated
umi TCGAAAACCG = 402 reads: +385 validated
umi TCGTTGGCGG = 393 reads: +385 validated
umi TGCCAAATCA = 273 reads: +385 validated
umi TGCCTAAGCT = 297 reads: +385 validated
umi TGGTAGAGGT = 314 reads: +385 validated
umi TGGTTGTCCC = 297 reads: +385 validated
umi TGTACACATG = 395 reads: +385 validated
umi TTAAACCTTT = 374 reads: +385 validated
umi TTAGCCTAAG = 414 reads: +385 validated
umi TTCTTTATTC = 255 reads: +385 validated
umi TTGGCCCTAG = 248 reads: +385 validated
umi TTTAGGGGTA = 373 reads: +385 validated

UMI info for barcode GCTGCAGCATATGGTC-1 contig 2 = AGCTCTGAGA...
umi AAAAGTGGGA = 30 reads: +317 -116 non-validated
umi AATACCGCAA = 151 reads: +433 validated
umi ATACCTTGCT = 250 reads: +433 validated
umi ATTGAACTCC = 255 reads: +433 validated
umi CAGGTATCTA = 70 reads: +423 -10 non-validated
umi CTGTGTAGTC = 273 reads: +433 validated
umi CTTAGCTCTG = 304 reads: +433 validated
umi GTCCATTTGT = 150 reads: +380 -8 +45 non-validated
umi TGTATTTGGG = 152 reads: +403 -30 non-validated
umi TTGGCTCCCT = 293 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=557]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 61 umis using 2752 reads
cdr3 = CQQYGSSPYTF at 360, score = 9 + 8
umis assigned: [16, 34, 68, 81, 93, 99, 111, 175, 222, 244] and 51 others
of which 61 are surviving nonsolos
reads assigned: 17984
start codons at 36, 244, 370, 463
confident = true

TIG 2[bases=583]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=2)
464-512 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
512-583 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 6 umis using 105 reads
cdr3 = CAKGKYGSGSYIGTDPFDYW at 421, score = 9 + 7
umis assigned: [6, 67, 216, 297, 359, 565, 573, 762, 1004, 1086]
of which 10 are surviving nonsolos
reads assigned: 1878
start codons at 79, 230, 235, 382, 437
confident = true
now this is a cell
paired!

GTATATTACTGTGCGAAAGGGAAATATGGTTCAGGGAGTTATATTGGGACGGATCCTTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.333 = GCTGCAGGTATAATGG-1

using 20727 reads

====================================================================================

graph has 9109 edges initially, 170 edges after simplification

total ucounts = 2058
nonsolo ucounts = 954[2^417, 3^206, 4^117, 5^68, 6^35, 7^19, 8^15, 9^9, 10^3, 11^4, 12^2, 13, 17, 18, 19, 20, 29, 35, 50, 58, 69, 83, 88, 96, 102, 107, 108, 116, 125, 127, 130, 132, 144, 160, 177, 199, 212, 214, 218, 233, 235, 237^3, 241, 243, 245, 251, 254^2, 267, 279, 291, 292, 301, 304, 309, 313, 328, 332, 338, 375, 390, 569, 631, 902, 906, 1002, 1010, 2049]
surviving nonsolo ucounts = 48[58, 69, 83, 88, 96, 102, 107, 108, 116, 125, 127, 132, 144, 177, 199, 212, 214, 218, 233, 235, 237^3, 241, 243, 245, 251, 254^2, 267, 279, 291, 292, 301, 304, 309, 313, 328, 332, 338, 390, 569, 631, 902, 906, 1002, 1010, 2049]
ids = [571, 140, 738, 61, 1488, 264, 2023, 188, 1782, 328, ...]

====================================================================================

UMI info for barcode GCTGCAGGTATAATGG-1 contig 1 = AGTCCCAGTC...
umi ATAAGTTTAT = 293 reads: +388 validated
umi CAGATTACGA = 141 reads: +388 validated
umi CATTTACTAG = 220 reads: +388 validated
umi CTAACGCCGC = 237 reads: +388 validated
umi GACTAATATG = 278 reads: +388 validated
umi GCGTTTCTAG = 336 reads: +388 validated
umi GGTCATCCCG = 301 reads: +388 validated
umi TCAGTGCTTT = 330 reads: +388 validated
umi TCGTGTGTAG = 256 reads: +388 validated
umi TCTTGGCTTT = 242 reads: +388 validated
umi TGCGTAGTCA = 334 reads: +388 validated
umi TTCCATCGAG = 313 reads: +388 validated
umi TTCCGCTGTG = 301 reads: +388 validated

UMI info for barcode GCTGCAGGTATAATGG-1 contig 2 = AGCTCTGAGA...
umi ACCATATCTA = 82 reads: +439 validated
umi ACGGATCATG = 217 reads: +439 validated
umi ACTGTGATCC = 71 reads: +397 -1 +10 -1 +1 -23 +6 non-validated
umi AGGACCGTAA = 108 reads: +427 -3 +1 -8 non-validated
umi ATCACCAGAC = 102 reads: +396 -43 non-validated
umi ATTGTCATGG = 128 reads: +439 validated
umi ATTGTCTCCT = 236 reads: +439 validated
umi CACTAACTAA = 225 reads: -368 +1 -9XX +5 -2XX +5 -2XX +2 -2XX +2 -1XX +3 -2XX +35 invalidated
umi CACTTCCCGC = 259 reads: +439 validated
umi CATAGTAGCG = 396 reads: +439 validated
umi CCAGACTATC = 243 reads: +439 validated
umi CCATCTTCTT = 62 reads: +408 -1 +9 -1 +5 -15 non-validated
umi CCCACAGCAA = 243 reads: +439 validated
umi CCTTTATTTA = 80 reads: +407 -1 +31 non-validated
umi CGCTTCAGAA = 132 reads: +439 validated
umi CTGGTCATAT = 172 reads: +439 validated
umi CTTGCCCTGA = 290 reads: +439 validated
umi CTTTAACCCT = 239 reads: +438 -1 non-validated
umi GCCCAGGATC = 211 reads: +439 validated
umi GCCGGTATAG = 185 reads: -378X +5 -2XX +5 -2XX +2 -2XX +2 -1XX +3 -2XX +35 invalidated
umi TATTGACCCA = 99 reads: +424 -15 non-validated
umi TCAACCTCTT = 246 reads: +439 validated
umi TGCTTTCCCT = 120 reads: +393 -1 +2 -2 +7 -1 +18 -15 non-validated
umi TTATCACCTG = 269 reads: +439 validated
umi TTCTATGCTC = 238 reads: +439 validated
umi TTGCCTAGGT = 198 reads: +429 -10 non-validated
umi TTTGCAACTC = 104 reads: +375 -1 +1 -6X +2 -1X +7 -1 +5 -1 +1 -1 +1 -1 +1 -2 +13 -1 +1 -1 +16 invalidated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 38-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
20-371 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=3)
370-408 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 13 umis using 586 reads
cdr3 = CQQLNSYPITF at 347, score = 9 + 8
umis assigned: [221, 426, 520, 831, 1039, 1160, 1233, 1537, 1677, 1730] and 3 others
of which 13 are surviving nonsolos
reads assigned: 3528
start codons at 20, 26, 82, 231, 450
confident = true

TIG 2[bases=589]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-288 ==> 0-209 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=2)
291-435 ==> 209-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=2)
467-518 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=4)
518-589 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 17 umis using 254 reads
cdr3 = CARDGVLPPLWSSGWRWFDPW at 424, score = 9 + 7
umis assigned: [61, 104, 140, 188, 264, 328, 329, 407, 416, 463] and 17 others
of which 26 are surviving nonsolos
reads assigned: 4842
start codons at 79, 235, 282, 385, 434
confident = true
see insertion of CAG at pos 209 on |120|IGHV3-21|L-REGION+V-REGION|

REJECT CONTIGS

TIG 1[bases=390]
0-37 ==> 0-37 on |391|IGLV9-49|5'UTR| [len=37] (mis=0)
37-181 ==> 0-144 on |392|IGLV9-49|L-REGION+V-REGION| [len=369] (mis=0)
179-390 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [18, 185, 478, 855, 868, 1299, 1500, 1565, 1664]
of which 9 are surviving nonsolos
reads assigned: 7110
start codons at 37
confident = false
did not find CDR3
now this is a cell
paired!

TATTACTGTGCGAGAGATGGGGTTCTACCCCCCCTTTGGAGCAGTGGCTGGCGCTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCGATCACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.337 = GCTGCAGGTCACTGGC-1

using 177 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 169]
surviving nonsolo ucounts = 1[169]
ids = [3]

====================================================================================

UMI info for barcode GCTGCAGGTCACTGGC-1 contig 1 = CTGGGAGAGG...
umi CTCGCCTATG = 166 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=558]
0-75 ==> 5-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
75-426 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=14)
463-511 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
511-558 ==> 0-47 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARESFAYCSADCHKYYFDYW at 417, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 162
start codons at 75, 231, 282, 292, 378
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.346 = GCTGCAGGTCTACCTC-1

using 225 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[221]
surviving nonsolo ucounts = 1[221]
ids = [3]

====================================================================================

UMI info for barcode GCTGCAGGTCTACCTC-1 contig 1 = AGCTGTGGGC...
umi GTCTGCTTCT = 216 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=527]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
384-422 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
422-527 ==> 0-105 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CNSRDSSGNHRVF at 355, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 212
start codons at 40, 159, 188, 239, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.356 = GCTGCAGGTGCCTGCA-1

using 431 reads

====================================================================================

graph has 226 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 4^2, 414]
surviving nonsolo ucounts = 1[414]
ids = [0]

====================================================================================

UMI info for barcode GCTGCAGGTGCCTGCA-1 contig 1 = AGGAACTGCT...
umi AGGTTAATGC = 421 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=550]
0-32 ==> 65-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
32-377 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=35)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CEQYNFWPRTF at 353, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 411
start codons at 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.359 = GCTGCAGGTGTTGAGG-1

using 176 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3, 4, 162]
surviving nonsolo ucounts = 1[162]
ids = [6]

====================================================================================

UMI info for barcode GCTGCAGGTGTTGAGG-1 contig 1 = AGCTTCAGCT...
umi GAAGTGAATC = 155 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=594]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=1)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=23)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
435-594 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CAAWDGGLTGVVF at 368, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 152
start codons at 47, 198, 258, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.370 = GCTGCAGTCACGAAGG-1

using 347 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[3, 92, 246]
surviving nonsolo ucounts = 2[92, 246]
ids = [8, 5]

====================================================================================

UMI info for barcode GCTGCAGTCACGAAGG-1 contig 1 = GAACGGAGGC...
umi GAGGCTTCTT = 245 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=585]
67-428 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
417-455 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
455-585 ==> 0-130 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CSSYTSSSTLVF at 391, score = 8 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 240
start codons at 23, 67, 224, 268, 275, 278
confident = false

REJECT CONTIGS

TIG 1[bases=303]
0-183 ==> 180-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=11)
181-220 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
220-303 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYYGSPRTF at 159, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 51
start codons at 142, 172, 262
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.373 = GCTGCAGTCACTGGGC-1

using 791 reads

====================================================================================

graph has 378 edges initially, 18 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[25, 144, 276, 343]
surviving nonsolo ucounts = 3[144, 276, 343]
ids = [2, 1, 4]

====================================================================================

UMI info for barcode GCTGCAGTCACTGGGC-1 contig 1 = CATGGACACA...
umi CAGATGAAGT = 138 reads: +433 validated

UMI info for barcode GCTGCAGTCACTGGGC-1 contig 2 = GGGGACTCCT...
umi CGACAAGCCC = 301 reads: +418 validated

UMI info for barcode GCTGCAGTCACTGGGC-1 contig 3 = AGAAGAGCTG...
umi ATAGTTGGTC = 263 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=510]
1-351 ==> 0-350 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=23)
382-434 ==> 0-52 on |49|IGHJ1|J-REGION| [len=52] (mis=4)
434-510 ==> 0-76 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 18 reads
cdr3 = CAGPWSSGFFATAEYFPHW at 346, score = 8 + 6
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 136
start codons at 1, 45, 224, 307
confident = false

TIG 2[bases=537]
20-370 ==> 0-350 on |94|IGHV2-26|L-REGION+V-REGION| [len=357] (mis=24)
387-438 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
438-537 ==> 0-99 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CASAAAPGDWFDPW at 365, score = 7 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 20, 176, 243, 335, 492
confident = false

TIG 3[bases=483]
0-34 ==> 18-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
34-382 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
380-419 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
419-483 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYGSSPVTF at 358, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 34, 242, 245, 368, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.383 = GCTGCAGTCCACGACG-1

using 251 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[4, 241]
surviving nonsolo ucounts = 1[241]
ids = [7]

====================================================================================

UMI info for barcode GCTGCAGTCCACGACG-1 contig 1 = GGAATCAGTC...
umi TAAGTTACGA = 241 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.391 = GCTGCAGTCCTCCTAG-1

using 184 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[184]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.394 = GCTGCAGTCGCCCTTA-1

using 43 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[39]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.397 = GCTGCAGTCGTTGACA-1

using 279 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 4, 270]
surviving nonsolo ucounts = 1[270]
ids = [4]

====================================================================================

UMI info for barcode GCTGCAGTCGTTGACA-1 contig 1 = GATCAGGACT...
umi TACCAGGTGC = 252 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=511]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-356 ==> 0-326 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=11)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
427-511 ==> 0-84 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CMNTLPGGFTF at 366, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.407 = GCTGCAGTCTGTCAAG-1

using 382 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[381]
surviving nonsolo ucounts = 1[381]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.417 = GCTGCGAAGACGCACA-1

using 462 reads

====================================================================================

graph has 216 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 4[4^2, 7, 436]
surviving nonsolo ucounts = 2[7, 436]
ids = [14, 9]

====================================================================================

UMI info for barcode GCTGCGAAGACGCACA-1 contig 1 = AGGAGTCAGA...
umi CGTGACCTGT = 439 reads: +388 validated
umi TTAAATGCCT = 7 reads: -28 +13 -1X +6 -1 +3 -1X +5 -1X +2 -2X +26 -1XX +2 -3XX +15 -1XX +11 -2 +10 -218 +1 -7X +1 -8X +1 -1X +1 -4X +1 -3X +1 -7X invalidated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 71 reads
cdr3 = CQQYNGQSRAF at 354, score = 8 + 7
umis assigned: [9, 14]
of which 2 are surviving nonsolos
reads assigned: 436
start codons at 27, 33, 102, 238, 241, 334, 367, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.433 = GCTGCGAAGGCGACAT-1

using 185 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3, 5, 9, 162]
surviving nonsolo ucounts = 1[162]
ids = [1]

====================================================================================

UMI info for barcode GCTGCGAAGGCGACAT-1 contig 1 = GGGGATCAGT...
umi ATGAATTACC = 146 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=443]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-443 ==> 0-28 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 146
start codons at 27, 33, 102, 238
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.434 = GCTGCGAAGGCGTACA-1

using 939 reads

====================================================================================

graph has 262 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 264, 669]
surviving nonsolo ucounts = 2[264, 669]
ids = [3, 4]

====================================================================================

UMI info for barcode GCTGCGAAGGCGTACA-1 contig 1 = GGGGTCTCAG...
umi TCATTTACCC = 255 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=565]
0-38 ==> 3-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=0)
38-375 ==> 0-337 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
388-426 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
426-565 ==> 0-139 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CSSYRHNNTLVF at 362, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 38, 189, 195, 246
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.435 = GCTGCGAAGGCTAGAC-1

using 413 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[178, 234]
surviving nonsolo ucounts = 2[178, 234]
ids = [2, 0]

====================================================================================

UMI info for barcode GCTGCGAAGGCTAGAC-1 contig 1 = TCTGGCGCCA...
umi ACTTACTTGT = 235 reads: +391 validated
umi TCACTTGGTT = 177 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=636]
0-34 ==> 0-34 on |385|IGLV7-43|5'UTR| [len=34] (mis=0)
34-385 ==> 0-351 on |386|IGLV7-43|L-REGION+V-REGION| [len=351] (mis=0)
387-425 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
425-636 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 64 reads
cdr3 = CLLYYGGAQRWVF at 358, score = 8 + 8
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 404
start codons at 34, 371
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.439 = GCTGCGAAGTAGCGGT-1

using 298 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[297]
surviving nonsolo ucounts = 1[297]
ids = [0]

====================================================================================

UMI info for barcode GCTGCGAAGTAGCGGT-1 contig 1 = GATCAGGACT...
umi AGTAACGCCC = 282 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=522]
0-30 ==> 5-35 on |276|IGKV2D-30|5'UTR| [len=35] (mis=0)
30-390 ==> 0-360 on |277|IGKV2D-30|L-REGION+V-REGION| [len=360] (mis=4)
392-430 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
430-522 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=1)
junction support: 1 umis using 41 reads
cdr3 = CMQGTHWPGITL at 366, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 30, 63, 91, 99, 159, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.447 = GCTGCGAAGTGGGCTA-1

using 222 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[222]
surviving nonsolo ucounts = 1[222]
ids = [0]

====================================================================================

UMI info for barcode GCTGCGAAGTGGGCTA-1 contig 1 = TGGGGACCCA...
umi ATCTCGGGCC = 212 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=605]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=6)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
461-495 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
495-605 ==> 0-110 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 401, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 59, 257, 262, 279, 323, 356, 549
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.456 = GCTGCGACAACGCACC-1

using 119 reads

====================================================================================

graph has 82 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[22, 94]
surviving nonsolo ucounts = 2[22, 94]
ids = [2, 4]

====================================================================================

UMI info for barcode GCTGCGACAACGCACC-1 contig 1 = CCCTGAGAGC...
umi GCACTATCAA = 87 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=514]
0-23 ==> 41-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
23-376 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=5)
399-447 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
447-514 ==> 0-67 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CARSLVSYSSSWIFDYW at 365, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 84
start codons at 23, 221, 226, 258, 287, 320
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.457 = GCTGCGACAAGACACG-1

using 17154 reads

====================================================================================

graph has 5892 edges initially, 48 edges after simplification

total ucounts = 727
nonsolo ucounts = 278[2^93, 3^60, 4^32, 5^12, 6^8, 7^5, 8^9, 9^2, 10, 13, 15, 16, 39, 45, 66, 68, 88, 121, 123, 184^2, 198, 199, 200, 203^2, 212, 214, 216, 232, 241, 249, 256, 257, 258, 263, 267, 272, 276, 283, 297, 305, 310, 314, 315, 318, 324, 332, 334, 342, 344, 347, 350, 354, 365, 367, 379, 381, 402^2, 408, 458, 589, 616, 1554]
surviving nonsolo ucounts = 51[39, 45, 66, 68, 123, 184^2, 198, 199, 200, 203^2, 212, 214, 216, 232, 241, 249, 256, 257, 258, 263, 267, 272, 276, 283, 297, 305, 310, 314, 315, 318, 324, 332, 334, 342, 344, 347, 350, 354, 365, 367, 379, 381, 402^2, 408, 458, 589, 616, 1554]
ids = [428, 221, 714, 155, 185, 6, 339, 647, 156, 45, ...]

====================================================================================

UMI info for barcode GCTGCGACAAGACACG-1 contig 1 = AGGTCTCAGA...
umi ACCACTATCC = 369 reads: +421 validated
umi ACGATAGGCT = 200 reads: +406 -1 +5 -1 +8 non-validated
umi ATTGCCAGCT = 64 reads: -418 +3 non-validated
umi ATTTAAATTG = 10 reads: -352 +1 -5XX +2 -4XX +1 -2XX +2 -2XX +2 -6XX +5 -1XX +1 -1XX +34 invalidated
umi CACTCTTACC = 212 reads: +421 validated
umi CAGACTGGGG = 87 reads: +419 -2 non-validated
umi CAGCTACCTC = 177 reads: +421 validated
umi CATACCCCGT = 198 reads: +421 validated
umi CATTATGCTT = 42 reads: -404X +17 invalidated
umi GCTGTTATCC = 36 reads: -379X +1 -16X +25 invalidated
umi TCCTTTTGGC = 215 reads: +421 validated
umi TGGACAATGT = 197 reads: +421 validated
umi TTTCCCCGCA = 56 reads: +25 -1 +376 -19 non-validated

UMI info for barcode GCTGCGACAAGACACG-1 contig 2 = GGGGAGGAAC...
umi AAAGGCGGGT = 187 reads: -7 +375 non-validated
umi AATGCGCTCG = 266 reads: +382 validated
umi ACCAGCTAAG = 194 reads: -355 +27 non-validated
umi ACTGTATCGT = 336 reads: +382 validated
umi ACTGTTAGCG = 1538 reads: -126X +1 -9X +1 -2XX +243 invalidated
umi AGGGGTTACC = 459 reads: +382 validated
umi AGGTATCCGA = 237 reads: +382 validated
umi ATGGTGTTTA = 236 reads: +382 validated
umi ATGTCAAAGT = 255 reads: -16X +1 -6X +1 -1XX +1 -5XX +1 -2XX +2 -1XX +1 -5XX +1 -2XX +336 invalidated
umi ATTTGAGGTG = 300 reads: +382 validated
umi CATAGGCTTA = 365 reads: +382 validated
umi CATTTGGCTG = 212 reads: +382 validated
umi CGTCATGGGG = 621 reads: +382 validated
umi CTACGATCAC = 594 reads: -136X +1 -2X +243 invalidated
umi CTAGCTTCCT = 382 reads: +382 validated
umi CTCTGTACTT = 315 reads: +382 validated
umi CTTACCGCCT = 184 reads: +382 validated
umi GATGCACGTT = 306 reads: +382 validated
umi GGAGAAGCCC = 273 reads: +382 validated
umi GTATCATATG = 398 reads: +382 validated
umi GTCGATAGCT = 282 reads: +6 -1XX +49 -1XX +8 -1XX +3 -1XX +15 -1XX +10 -1XX +56 -1XX +63 -1XX +12 -1XX +7 -1XX +30 -1XX +20 -1XX +3 -1XX +2 -1XX +16 -1XX +18 -2XX +2 -1XX +2 -2XX +4 -2XX +34 invalidated
umi TAATCTGTAC = 397 reads: +382 validated
umi TAATTTCCGT = 349 reads: +382 validated
umi TATACGTAAT = 14 reads: -314 +1 -1XX +1 -2XX +1 -5XX +1 -5XX +1 -9XX +1 -5XX +35 invalidated
umi TATCTATCCT = 353 reads: +382 validated
umi TATCTTGTGC = 288 reads: +382 validated
umi TCACAGCTCT = 255 reads: +382 validated
umi TCCGAGACCG = 17 reads: -236 +1 -1X +1 -1XX +17 -1XX +11 -1XX +20 -1XX +5 -2XX +14 -2XX +3 -1XX +3 -61 invalidated
umi TCGGACCACA = 349 reads: +382 validated
umi TCTACATCCG = 409 reads: +382 validated
umi TGCATGGGTA = 333 reads: +382 validated
umi TGTAAGATGT = 345 reads: +382 validated
umi TTACGGCGCT = 270 reads: +382 validated
umi TTGCTCCTGT = 351 reads: +382 validated
umi TTGGTCCATT = 311 reads: +382 validated
umi TTGTCATCCA = 383 reads: +382 validated
umi TTTAGCTCGG = 322 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=602]
0-79 ==> 0-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=2)
452-500 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
500-602 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 6 umis using 131 reads
cdr3 = CAREGDGYNFVYFDYW at 421, score = 9 + 7
umis assigned: [44, 61, 155, 156, 179, 185, 193, 205, 221, 428] and 3 others
of which 13 are surviving nonsolos
reads assigned: 1823
start codons at 79, 235, 296, 314, 382, 437, 518, 579
confident = true

TIG 2[bases=554]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 33 umis using 2074 reads
cdr3 = CQQYNNWPPTF at 357, score = 9 + 8
umis assigned: [6, 26, 45, 74, 76, 102, 103, 146, 148, 158] and 27 others
of which 37 are surviving nonsolos
reads assigned: 12465
start codons at 36, 105, 241, 460
confident = true
now this is a cell
paired!

GAGGACACGGCTGTGTATTACTGTGCGAGAGAAGGAGATGGCTACAATTTTGTGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGGCCTCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.462 = GCTGCGACAATCCGAT-1

using 224 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[223]
surviving nonsolo ucounts = 1[223]
ids = [1]

====================================================================================

UMI info for barcode GCTGCGACAATCCGAT-1 contig 1 = GCTCTGCTTC...
umi GGTCGATCCC = 211 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=555]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
442-555 ==> 0-113 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQSYDTSLSGSVF at 375, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.464 = GCTGCGACAATGGAGC-1

using 35 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 4, 6^2, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.486 = GCTGCGACAGTCAGCC-1

using 12788 reads

====================================================================================

graph has 9908 edges initially, 190 edges after simplification

total ucounts = 2285
nonsolo ucounts = 1732[2^251, 3^201, 4^161, 5^160, 6^150, 7^122, 8^116, 9^114, 10^86, 11^91, 12^78, 13^64, 14^34, 15^31, 16^20, 17^11, 18^13, 19^2, 20^7, 21^2, 22^3, 23^5, 24^2, 25^2, 26^2, 27^2, 30, 32]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.491 = GCTGCGACATAGTAAG-1

using 2125 reads

====================================================================================

graph has 2871 edges initially, 28 edges after simplification

total ucounts = 1121
nonsolo ucounts = 439[2^217, 3^95, 4^50, 5^34, 6^16, 7^8, 8^4, 9^5, 10^2, 11^3, 12, 13^2, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.500 = GCTGCGACATCTCCCA-1

using 17112 reads

====================================================================================

graph has 12908 edges initially, 214 edges after simplification

total ucounts = 2400
nonsolo ucounts = 1814[2^317, 3^240, 4^171, 5^159, 6^156, 7^134, 8^130, 9^84, 10^78, 11^71, 12^63, 13^53, 14^38, 15^26, 16^20, 17^14, 18^15, 19^7, 20^2, 21^3, 22^6, 23^4, 24^2, 26, 28, 31, 32, 43, 64, 66, 179, 238, 255, 275, 290, 302, 308, 326, 342, 359, 362, 365, 414, 454]
surviving nonsolo ucounts = 15[66, 179, 238, 255, 275, 290, 302, 308, 326, 342, 359, 362, 365, 414, 454]
ids = [1355, 1955, 286, 1969, 1167, 812, 2334, 2212, 1940, 1959, ...]

====================================================================================

UMI info for barcode GCTGCGACATCTCCCA-1 contig 1 = AGTGACTCCT...
umi AAGTGACATA = 467 reads: +436 validated
umi CGCCCTTCAC = 362 reads: +436 validated
umi GTACGAATCT = 349 reads: +436 validated

UMI info for barcode GCTGCGACATCTCCCA-1 contig 2 = GGGGGTGCAG...
umi ACGGACACGC = 250 reads: +316 -4XX +2 -3X +63 invalidated
umi ATTACTCCAC = 328 reads: -358X +2 -3XX +2 -3XX +1 -3XX +1 -1XX +1 -1XX +1 -3XX +1 -1XX +2 -4XX invalidated
umi CCAGCTTAAA = 278 reads: -358X +2 -3XX +2 -3XX +1 -3XX +1 -1XX +1 -1XX +1 -3XX +1 -1XX +2 -4XX invalidated
umi CGGTACACTG = 274 reads: +388 validated
umi CTCTGAGTTG = 350 reads: +388 validated
umi CTGGCATCTG = 60 reads: +388 validated
umi CTTGTCTATG = 1 reads: -388 non-validated
umi GCTCACGATG = 1 reads: -388 non-validated
umi GGTTGCCTAG = 329 reads: +388 validated
umi GTACCACATA = 177 reads: +388 validated
umi GTAGTTTCGG = 257 reads: +388 validated
umi TCGAACCAAT = 286 reads: -358X +2 -3XX +2 -3XX +1 -3XX +1 -1XX +1 -1XX +1 -3XX +1 -1XX +2 -4XX invalidated
umi TGACGCCCTA = 416 reads: +388 validated
umi TTATCCCTTT = 288 reads: -358X +2 -3XX +2 -3XX +1 -3XX +1 -1XX +1 -1XX +1 -3XX +1 -1XX +2 -4XX invalidated

GOOD CONTIGS

TIG 1[bases=527]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=16)
436-456 ==> 32-52 on |49|IGHJ1|J-REGION| [len=52] (mis=1)
456-527 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 84 reads
cdr3 = CAHISTLFRSAYYRPYLDFW at 365, score = 7 + 6
umis assigned: [118, 1097, 1959]
of which 3 are surviving nonsolos
reads assigned: 1144
start codons at 20, 64, 243, 246, 326, 335
confident = true

TIG 2[bases=559]
35-388 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=12)
386-423 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 7 umis using 279 reads
cdr3 = CQQGYTTPLSF at 362, score = 9 + 8
umis assigned: [286, 574, 812, 1167, 1304, 1355, 1428, 1707, 1940, 1955] and 4 others
of which 12 are surviving nonsolos
reads assigned: 3245
start codons at 35, 41, 97, 110, 246, 465
confident = true
now this is a cell
paired!

ACATATTACTGTGCGCACATCTCCACTCTTTTCCGGAGTGCTTATTATCGGCCCTACTTAGATTTTTGGGGCCAGGGAGTCCTGGTCTCCGTCTCCTCAG <==> ATCAACTGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGGGTTACACTACCCCTCTCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.512 = GCTGCGAGTACGACCC-1

using 12152 reads

====================================================================================

graph has 3905 edges initially, 54 edges after simplification

total ucounts = 392
nonsolo ucounts = 162[2^51, 3^28, 4^15, 5^6, 6^5, 7^4, 8^3, 9^2, 10^2, 11, 12, 18, 23, 54, 76, 84, 90, 160, 165, 167, 193, 212, 223, 239, 245, 249, 260, 264, 265, 266, 275, 278, 280, 284, 285, 289, 292^2, 293, 295, 296^2, 305, 319, 321, 325, 331, 332, 347, 348, 357, 359, 417, 422, 612]
surviving nonsolo ucounts = 42[54, 76, 84, 90, 160, 165, 167, 193, 212, 223, 239, 245, 249, 260, 264, 265, 266, 275, 278, 280, 284, 285, 289, 292^2, 293, 295, 296^2, 305, 319, 321, 325, 331, 332, 347, 348, 357, 359, 417, 422, 612]
ids = [139, 253, 317, 264, 318, 134, 176, 308, 326, 40, ...]

====================================================================================

UMI info for barcode GCTGCGAGTACGACCC-1 contig 1 = AGAGCTCTGG...
umi AAAATGCCAA = 333 reads: +385 validated
umi ACCCTAACTA = 284 reads: +385 validated
umi ACCGCATGAG = 349 reads: +385 validated
umi ACTATTGTGG = 358 reads: +385 validated
umi ACTCTGTACT = 291 reads: +385 validated
umi AGGCATTGTA = 292 reads: +385 validated
umi ATCGCCTGGT = 361 reads: +385 validated
umi ATGTGTACCG = 331 reads: +385 validated
umi CAATTCATCC = 320 reads: +385 validated
umi CCGCATACTC = 295 reads: +385 validated
umi CCTCGCTCCT = 424 reads: +385 validated
umi CGTTGAGTTC = 166 reads: +385 validated
umi CTATGCCCGT = 269 reads: +385 validated
umi CTTGATTCCG = 268 reads: +385 validated
umi GCGAGCGAAC = 416 reads: +385 validated
umi GGGCTCCACA = 644 reads: +146 -1XX +5 -1XX +1 -10XX +4 -3XX +1 -128X +3 -9XX +1 -4XX +2 -3XX +1 -1XX +61 invalidated
umi GGTGACATCT = 317 reads: +385 validated
umi GTGTTATCGG = 252 reads: +385 validated
umi TACCTCAGGT = 265 reads: +385 validated
umi TAGTTTACCG = 297 reads: +385 validated
umi TCCATTATAT = 306 reads: +385 validated
umi TCGAGCACCG = 216 reads: +385 validated
umi TTAGGCCTGG = 297 reads: +385 validated

UMI info for barcode GCTGCGAGTACGACCC-1 contig 2 = GGGGGACCCA...
umi AAACCAACAG = 278 reads: +424 validated
umi AAAGGAGCTC = 262 reads: +424 validated
umi AATTTAGTTG = 220 reads: +424 validated
umi AGCATTTCTT = 235 reads: +424 validated
umi ATGAAATCTC = 295 reads: +424 validated
umi CAGGGTCCAC = 140 reads: +424 validated
umi CATCCTCCGT = 279 reads: +424 validated
umi CATGCATACG = 46 reads: +409 -15 non-validated
umi CATGCGGCCC = 351 reads: +424 validated
umi CGGGGATAGC = 291 reads: +424 validated
umi GAACTGCTGT = 213 reads: +424 validated
umi GGATGGCGGC = 63 reads: +424 validated
umi TATCGCGCTG = 167 reads: +424 validated
umi TCAAGTTTTT = 270 reads: +424 validated
umi TCAGGCCTTT = 83 reads: +400 -24 non-validated
umi TCATCGTGCC = 160 reads: +424 validated
umi TTCGCTCATA = 278 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 22 umis using 987 reads
cdr3 = CQQYGSSLGTF at 368, score = 9 + 8
umis assigned: [0, 56, 58, 67, 71, 89, 101, 108, 120, 153] and 13 others
of which 23 are surviving nonsolos
reads assigned: 7216
start codons at 44, 252, 378, 471
confident = true

TIG 2[bases=554]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=4)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=2)
435-483 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
483-554 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 14 umis using 249 reads
cdr3 = CARALGSAPRLLPVDYW at 401, score = 8 + 7
umis assigned: [2, 4, 40, 80, 103, 134, 136, 139, 140, 168] and 7 others
of which 17 are surviving nonsolos
reads assigned: 3572
start codons at 59, 210, 257, 262, 279, 294, 323, 356
confident = true
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGAGCCTTAGGTTCCGCTCCAAGGCTGCTCCCCGTCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCTCTGGGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.538 = GCTGCGAGTGTGTGCC-1

using 356 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[356]
surviving nonsolo ucounts = 1[356]
ids = [0]

====================================================================================

UMI info for barcode GCTGCGAGTGTGTGCC-1 contig 1 = GGGGAGGAAC...
umi TAGGCAGAGT = 366 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=17)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CLQYSDWPRTF at 357, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 356
start codons at 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.539 = GCTGCGAGTGTTGAGG-1

using 839 reads

====================================================================================

graph has 1236 edges initially, 14 edges after simplification

total ucounts = 477
nonsolo ucounts = 163[2^90, 3^30, 4^15, 5^14, 6^2, 7^5, 8^2, 11^2, 12, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.542 = GCTGCGAGTTCAGACT-1

using 366 reads

====================================================================================

graph has 188 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 358]
surviving nonsolo ucounts = 1[358]
ids = [6]

====================================================================================

UMI info for barcode GCTGCGAGTTCAGACT-1 contig 1 = GGGGATCAGT...
umi GATATTCCAA = 363 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 69 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 353
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.556 = GCTGCGATCACCTCGT-1

using 256 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 248]
surviving nonsolo ucounts = 1[248]
ids = [1]

====================================================================================

UMI info for barcode GCTGCGATCACCTCGT-1 contig 1 = ATCAGTCCCA...
umi ACACCCTCCC = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.564 = GCTGCGATCCGCAAGC-1

using 132 reads

====================================================================================

graph has 132 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2, 3, 4, 5, 6^3, 7, 8^3, 9^2, 13^2, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.579 = GCTGCGATCTAAGCCA-1

using 198 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[3, 4, 186]
surviving nonsolo ucounts = 1[186]
ids = [4]

====================================================================================

UMI info for barcode GCTGCGATCTAAGCCA-1 contig 1 = CCACTCAGGA...
umi AGCTGTGCGG = 173 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=452]
16-367 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
366-404 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
404-452 ==> 0-48 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CLQYSSSPWTF at 343, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 171
start codons at 16, 22, 91, 227, 446
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.595 = GCTGCTTAGAAGGGTA-1

using 1081 reads

====================================================================================

graph has 478 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2, 123, 265, 331, 353]
surviving nonsolo ucounts = 4[123, 265, 331, 353]
ids = [4, 1, 8, 6]

====================================================================================

UMI info for barcode GCTGCTTAGAAGGGTA-1 contig 1 = CTGACTACCA...
umi CAAGAATTTT = 269 reads: +382 validated
umi CGGCTACTCA = 126 reads: +382 validated
umi GCCATCGAAT = 30 reads: -348X +2 -1XX +6 -2XX +15 -1XX +4 -1XX +2 invalidated
umi TAGTCATAAT = 331 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=550]
0-32 ==> 31-63 on |297|IGKV5-2|5'UTR| [len=63] (mis=0)
32-377 ==> 0-345 on |298|IGKV5-2|L-REGION+V-REGION| [len=345] (mis=5)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 3 umis using 123 reads
cdr3 = CLQHDNFPWTF at 353, score = 7 + 8
umis assigned: [1, 4, 6, 8]
of which 4 are surviving nonsolos
reads assigned: 738
start codons at 32, 122, 180, 183, 188, 291, 336, 363, 456
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.611 = GCTGCTTAGATAGGAG-1

using 15 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.615 = GCTGCTTAGCACCGCT-1

using 252 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 4, 243]
surviving nonsolo ucounts = 1[243]
ids = [2]

====================================================================================

UMI info for barcode GCTGCTTAGCACCGCT-1 contig 1 = GCTGGGGTCT...
umi TACTTCCATC = 238 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=594]
0-41 ==> 0-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=0)
41-402 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=5)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
435-594 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CSSYTSSSTLEVVF at 365, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 232
start codons at 41, 198, 242, 249
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.618 = GCTGCTTAGCAGGCTA-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.619 = GCTGCTTAGCCACGCT-1

using 133 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[131]
surviving nonsolo ucounts = 1[131]
ids = [0]

====================================================================================

UMI info for barcode GCTGCTTAGCCACGCT-1 contig 1 = CTTCAGCTGT...
umi CTTGGTGATT = 127 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=506]
0-45 ==> 6-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
45-401 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
417-436 ==> 19-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
436-506 ==> 0-70 on |305|IGLC1|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CQSYDSSLSGYVF at 369, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 126
start codons at 45, 199, 253, 352, 379, 400
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.625 = GCTGCTTAGCTAGGCA-1

using 804 reads

====================================================================================

graph has 260 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 3, 792]
surviving nonsolo ucounts = 1[792]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.630 = GCTGCTTAGGATGGTC-1

using 999 reads

====================================================================================

graph has 308 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2^2, 3, 224, 250, 510]
surviving nonsolo ucounts = 2[224, 510]
ids = [7, 11]

====================================================================================

UMI info for barcode GCTGCTTAGGATGGTC-1 contig 1 = GGGAATCAGT...
umi CAGCTACTGA = 48 reads: -252 +2 -1XX +3 -2XX +3 -1XX +11 -1XX +11 -1XX +29 -3XX +11 -2XX +3 -1XX +3 -1XX +2 -2XX +1 -2XX +2 -1XX +1 -2XX +2 -1XX +8 -1XX +9 -1XX +4 -1XX +7 invalidated
umi CGTCGGGGTA = 224 reads: +388 validated
umi TAGCACGTAC = 506 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 118 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [2, 7, 11]
of which 2 are surviving nonsolos
reads assigned: 769
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.638 = GCTGCTTAGGTAGCCA-1

using 313 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[3, 5, 299]
surviving nonsolo ucounts = 1[299]
ids = [3]

====================================================================================

UMI info for barcode GCTGCTTAGGTAGCCA-1 contig 1 = GAGGAGTCAG...
umi AGAAAAACGT = 299 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=4)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQQSYSTQWTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 28, 34, 90, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.651 = GCTGCTTAGTTCGCAT-1

using 327 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[327]
surviving nonsolo ucounts = 1[327]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.670 = GCTGCTTCAATGCCAT-1

using 357 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 112, 235]
surviving nonsolo ucounts = 2[112, 235]
ids = [8, 3]

====================================================================================

UMI info for barcode GCTGCTTCAATGCCAT-1 contig 1 = TATTGGGGCT...
umi GCGCTTTGTC = 230 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=552]
21-392 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=7)
409-457 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
457-552 ==> 0-95 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CARAHGDYYTLLDCW at 381, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 21, 42, 86, 172, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.675 = GCTGCTTCACACAGAG-1

using 242 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 4^2, 227]
surviving nonsolo ucounts = 1[227]
ids = [3]

====================================================================================

UMI info for barcode GCTGCTTCACACAGAG-1 contig 1 = TGGGGAGGAA...
umi ATTTGGAGCC = 228 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.681 = GCTGCTTCACATGACT-1

using 45933 reads

====================================================================================

graph has 19049 edges initially, 228 edges after simplification

total ucounts = 3240
nonsolo ucounts = 1432[2^598, 3^291, 4^160, 5^83, 6^51, 7^30, 8^21, 9^11, 10^4, 11^4, 12^6, 13^3, 14^6, 15^2, 16^3, 17^4, 19, 22^2, 24, 27^2, 28, 34, 36, 43^2, 44, 47, 52, 53^2, 54, 59, 61, 68, 69, 73, 76, 87, 100, 105, 108, 116, 117, 125, 126, 136, 141, 142, 145, 146, 147, 148, 154, 159^2, 162^3, 174, 178, 180^2, 182, 183, 187, 188, 190, 192^2, 193, 196, 197, 200, 205, 207^3, 208, 213, 214, 215, 216, 219, 221, 222, 223, 225, 226, 228, 230, 231, 232, 233^2, 235, 237, 238^2, 241, 245, 246, 249, 250, 252^2, 254, 256, 258, 259, 260^2, 261^2, 262^2, 264^2, 266, 270, 272, 273, 274, 276, 277, 279, 284, 286^2, 290, 292, 294, 295, 310, 315, 321, 323, 328, 332, 334, 335, 336, 337, 340, 354, 357, 359, 381, 385, 417, 435, 442, 454, 478, 485, 487, 516, 526, 566, 569, 579, 590, 606, 695, 758, 800, 831, 856, 932, 997]
surviving nonsolo ucounts = 143[13, 19, 27, 47, 52, 53, 59, 61, 68, 69, 73, 87, 100, 105, 108, 116, 117, 125, 126, 136, 141, 142, 145, 146, 147, 148, 154, 159^2, 162^3, 174, 178, 180^2, 182, 183, 187, 188, 190, 192^2, 193, 196, 197, 200, 205, 207^3, 208, 213, 214, 215, 216, 219, 221, 222, 223, 225, 226, 228, 230, 231, 232, 233^2, 235, 237, 238^2, 241, 245, 246, 249, 250, 252^2, 254, 256, 258, 259, 260^2, 261^2, 262^2, 264^2, 266, 270, 272, 273, 274, 276, 277, 279, 284, 286^2, 290, 292, 294, 295, 310, 315, 321, 323, 328, 332, 334, 335, 336, 337, 340, 354, 357, 359, 381, 385, 417, 435, 442, 454, 478, 485, 487, 516, 526, 566, 569, 579, 590, 606, 695, 758, 800, 831, 856, 932, 997]
ids = [2287, 1790, 1608, 785, 1880, 2198, 2140, 388, 362, 1150, ...]

====================================================================================

UMI info for barcode GCTGCTTCACATGACT-1 contig 1 = TGGGGAAAAC...
umi AACACCTACG = 859 reads: -168 +35 -1 +1 -2 +3 -2 +1 -1 +5 -2 +3 -64X +100 invalidated
umi AACTTCCGGC = 274 reads: +388 validated
umi AACTTTAGCG = 255 reads: +388 validated
umi AAGTTTGCGT = 194 reads: +388 validated
umi ACAAATTCCG = 195 reads: +388 validated
umi ACACGACTCC = 290 reads: +388 validated
umi ACAGGTTTAT = 247 reads: +388 validated
umi ACGTAATATC = 68 reads: +388 validated
umi ACTGTTACGG = 263 reads: +388 validated
umi AGACTGTCGT = 189 reads: +388 validated
umi AGCATCTCTG = 292 reads: +388 validated
umi AGCGATCGCA = 110 reads: +388 validated
umi AGCGGAGTGC = 196 reads: +14 -4X +3 -5X +3 -7XX +1 -1XX +4 -1XX +345 invalidated
umi ATGAAGCCTG = 163 reads: +388 validated
umi ATTACCTCAC = 695 reads: -214X +174 invalidated
umi ATTAGGGGTC = 226 reads: +388 validated
umi ATTCAGTACC = 260 reads: +388 validated
umi ATTCCGACAC = 263 reads: +388 validated
umi ATTGAAGCGC = 932 reads: -300X +1 -1X +86 invalidated
umi CAATGGGCTT = 3 reads: -345X +1 -1XX +1 -4XX +1 -1XX +2 -1XX +5 -4XX +8 -2XX +1 -1XX +10 invalidated
umi CACAACTGCA = 4 reads: -388 non-validated
umi CACGTGCCTA = 171 reads: +388 validated
umi CAGGACGCTC = 206 reads: +388 validated
umi CATGCGGGTC = 2 reads: -350 +2 -2XX +2 -1XX +5 -4XX +8 -2XX +1 -1XX +10 invalidated
umi CCATAGGACG = 225 reads: +388 validated
umi CCGGCTTGGC = 70 reads: +327 -1 +60 non-validated
umi CCGTACACCT = 179 reads: +388 validated
umi CCGTGTAGTT = 133 reads: +388 validated
umi CCTACAGAGC = 213 reads: +388 validated
umi CGAGGGTCCT = 278 reads: +388 validated
umi CGCCGCTCCA = 282 reads: +388 validated
umi CGGCCCCTTT = 128 reads: -9 +1 -5XX +2 -7XX +1 -4XX +2 -9XX +1 -3XX +344 invalidated
umi CTAAGAGCGT = 190 reads: +388 validated
umi CTACATATTA = 5 reads: -388 non-validated
umi CTGAAACTCG = 217 reads: +388 validated
umi CTGCCCCCTC = 107 reads: -388 non-validated
umi CTGGCGTTGC = 254 reads: +388 validated
umi CTTCACCCCT = 253 reads: +388 validated
umi GAACAGCATT = 269 reads: +388 validated
umi GACACCTAGG = 277 reads: +388 validated
umi GAGCTAGGTG = 140 reads: +388 validated
umi GCCTGGGGAC = 186 reads: +388 validated
umi GCGTCCAGTC = 114 reads: +388 validated
umi GGCATCCCAA = 270 reads: +388 validated
umi GGGAAGCCTG = 303 reads: +388 validated
umi GGGGCGCTGG = 210 reads: +388 validated
umi GTCCAATGGG = 181 reads: +388 validated
umi GTCTTCTTCG = 315 reads: +388 validated
umi GTTCCTTGAT = 262 reads: +388 validated
umi GTTTGTTGGG = 248 reads: +388 validated
umi TAAAGCACCT = 125 reads: +388 validated
umi TAAGAGGTGT = 18 reads: -354 +1 -4X +2 -4XX +2 -4XX +1 -2XX +1 -6XX +1 -4XX +2 invalidated
umi TATGGTTACC = 238 reads: +388 validated
umi TCATCCTCCG = 280 reads: +388 validated
umi TCCCCTTTCA = 178 reads: -388 non-validated
umi TCCGTTGCTC = 266 reads: +388 validated
umi TCGCCCGGCC = 494 reads: +388 validated
umi TGCATTTCGC = 236 reads: +388 validated
umi TGCTAATCCC = 245 reads: +388 validated
umi TGCTCTCTTC = 234 reads: +388 validated
umi TGGGAGACGT = 194 reads: +388 validated
umi TGGGATAATT = 250 reads: +388 validated
umi TGTACCCCGT = 193 reads: +388 validated
umi TGTATTTAGT = 217 reads: +388 validated
umi TTACCCCGCC = 208 reads: +388 validated
umi TTCAACCCTT = 245 reads: +388 validated
umi TTCCATCCAG = 565 reads: -363 +25 non-validated
umi TTCGATTTAC = 260 reads: +388 validated
umi TTCTTATCCC = 294 reads: +388 validated
umi TTCTTTTTGG = 234 reads: +388 validated
umi TTTAGGTCCA = 128 reads: +388 validated
umi TTTGCACGTC = 220 reads: +388 validated

UMI info for barcode GCTGCTTCACATGACT-1 contig 2 = GAGCTCTGGG...
umi AAATGTATGA = 427 reads: +49 -5XX +2 -2XX +1 -7XX +1 -1XX +1 -3XX +4 -131XX +1 -1XX +1 -2XX +1 -2XX +1 -4XX +1 -1XX +1 -2XX +4 -1XX +1 -5XX +2 -3XX +165 invalidated
umi ACTCAGCCTA = 58 reads: +331 -7 +68 non-validated
umi ACTCGTCCGT = 229 reads: +406 validated
umi AGCCTGCATC = 129 reads: -366 +2 -3X +9 -1X +5 -1X +1 -1XX +12 -1XX +4 invalidated
umi AGGTATTGGC = 517 reads: -370X +10 -1XX +5 -1XX +1 -1XX +12 -1XX +4 invalidated
umi AGGTTACGTC = 224 reads: +406 validated
umi ATGACCTATT = 82 reads: +406 validated
umi ATGTCATGCT = 232 reads: +406 validated
umi ATTCATACAT = 354 reads: -370X +10 -1XX +5 -1XX +1 -1XX +12 -1XX +4 invalidated
umi ATTTAGCGCG = 50 reads: +317 -1X +31 -7 +50 invalidated
umi CACTACGGGT = 195 reads: +406 validated
umi CATAAATTGG = 218 reads: +406 validated
umi CCATTAATTG = 265 reads: +279 -1XX +126 invalidated
umi CCGATCATTA = 500 reads: +49 -5XX +2 -2XX +1 -7XX +1 -1XX +1 -3XX +4 -5XX +1 -125XX +1 -1XX +1 -2XX +1 -2XX +1 -4XX +1 -1XX +1 -2XX +4 -1XX +1 -5XX +2 -3XX +165 invalidated
umi CTCGGACCCC = 147 reads: +406 validated
umi CTCGGCGTGG = 397 reads: +49 -5XX +2 -2XX +1 -7XX +1 -1XX +1 -3XX +4 -5XX +1 -125X +1 -1XX +1 -2XX +1 -2XX +1 -4XX +1 -1XX +1 -2XX +4 -1XX +1 -5XX +2 -3XX +165 invalidated
umi GCACAGGTCT = 415 reads: -372X +1 -1X +1 -8X +1 -11XX +2 -1XX +1 -6XX +1 invalidated
umi GCGCTACTTC = 343 reads: +406 validated
umi GCTAATCGTT = 51 reads: +304 -1XX +101 invalidated
umi GTACTTCTAT = 214 reads: +406 validated
umi GTCATCTAGA = 59 reads: -16 +375 -15 non-validated
umi GTGGAGGTCC = 51 reads: -406 non-validated
umi GTTATGTATT = 138 reads: +369 -6 +31 non-validated
umi TACATACGTA = 298 reads: -358X +1 -1X +1 -1X +1 -1X +1 -1X +2 -2X +10 -1XX +5 -1XX +1 -1XX +12 -1X +4 invalidated
umi TAGAACGCTA = 319 reads: +406 validated
umi TCAGTTTCGC = 154 reads: +406 validated
umi TCCTAACTGG = 189 reads: +406 validated
umi TCTATATTAC = 584 reads: +172 -5XX +2 -10XX +1 -216XX invalidated
umi TGCAGGACCT = 407 reads: -374X +1 -1X +2 -3XX +1 -6XX +3 -1XX +1 -3XX +2 -2XX +1 -2XX +3 invalidated
umi TGGAATCTCT = 339 reads: -371 +9 -1XX +5 -1XX +1 -1XX +12 -1XX +4 invalidated
umi TTTCTCAGTA = 91 reads: -406 non-validated

GOOD CONTIGS

TIG 1[bases=642]
43-395 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=19)
393-431 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
431-642 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 63 umis using 2545 reads
cdr3 = CSAWDSSLNVWVF at 364, score = 7 + 8
umis assigned: [50, 109, 112, 163, 222, 240, 260, 362, 420, 456] and 62 others
of which 72 are surviving nonsolos
reads assigned: 16723
start codons at 43, 182, 372, 389
confident = true

TIG 2[bases=557]
0-80 ==> 0-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=21)
436-486 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
486-557 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 17 umis using 396 reads
cdr3 = CVKEEDAFDIW at 422, score = 9 + 8
umis assigned: [40, 388, 402, 496, 544, 552, 715, 737, 764, 785] and 21 others
of which 31 are surviving nonsolos
reads assigned: 7548
start codons at 80, 231, 236, 297, 315, 383, 438, 467
confident = true

REJECT CONTIGS

TIG 1[bases=557]
5-80 ==> 5533-5608 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=2)
35-231 ==> 0-196 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
231-382 ==> 209-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=3) [SHIFT!]
383-421 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CMQALQTPRSPLAK at 358, score = 8 + 4
umis assigned: [20, 84, 231, 342, 554, 581, 587, 656, 744, 1102] and 5 others
of which 15 are surviving nonsolos
reads assigned: 4472
start codons at 35, 68, 104, 192, 341, 361, 463
confident = false
not full
frameshifted full length stopped transcript of length 557
VJ delta = 34
not full
now this is a cell
paired!

AGCGGCCTGAGAGCTGAGGACACGGCTCTTTATTACTGTGTTAAAGAGGAGGATGCTTTTGATATCTGGGGCCAGGGGACAATGGTCACCGTCTCTTCAG <==> GGACTCCAGCCTGACGACGAGGGTGACTATTACTGCTCAGCATGGGACAGCAGCCTCAATGTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.686 = GCTGCTTCACCGTTGG-1

using 187 reads

====================================================================================

graph has 88 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 6[2, 3^2, 4, 7, 160]
surviving nonsolo ucounts = 1[160]
ids = [1]

====================================================================================

UMI info for barcode GCTGCTTCACCGTTGG-1 contig 1 = GGGGAGGAAC...
umi ACGAAACGTC = 161 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=2)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQRSNWQLTF at 357, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 36, 241, 244, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.699 = GCTGCTTCACTCTGTC-1

using 268 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 3, 262]
surviving nonsolo ucounts = 1[262]
ids = [3]

====================================================================================

UMI info for barcode GCTGCTTCACTCTGTC-1 contig 1 = GATCAGGACT...
umi CCCAAATGCA = 253 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=473]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-356 ==> 0-326 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=7)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
427-473 ==> 0-46 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CMQSLQMPLTF at 366, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 30, 63, 99, 187, 349, 369, 384, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.718 = GCTGCTTCAGGACCCT-1

using 15578 reads

====================================================================================

graph has 5412 edges initially, 58 edges after simplification

total ucounts = 1100
nonsolo ucounts = 466[2^199, 3^100, 4^47, 5^27, 6^16, 7^8, 8^6, 9^4, 10^2, 11, 12, 16, 17, 21, 24, 46, 70, 79, 107, 119, 157, 161, 166, 178, 180, 190, 198, 210, 211, 234, 238, 253, 254, 261, 263^2, 269, 273, 278, 286, 288, 292, 300, 303, 307^2, 310, 311, 313, 317, 319, 320, 321, 326, 333, 334, 345, 347, 348, 355, 360^2, 364, 368, 376, 397]
surviving nonsolo ucounts = 49[79, 107, 119, 157, 161, 166, 178, 180, 190, 198, 210, 211, 234, 238, 253, 254, 261, 263^2, 269, 273, 278, 286, 288, 292, 300, 303, 307^2, 310, 311, 313, 317, 319, 320, 321, 326, 333, 334, 345, 347, 348, 355, 360^2, 364, 368, 376, 397]
ids = [1083, 466, 528, 739, 509, 210, 393, 615, 1053, 11, ...]

====================================================================================

UMI info for barcode GCTGCTTCAGGACCCT-1 contig 1 = AGGAGTCAGT...
umi AAATCTACGG = 202 reads: +388 validated
umi ACAACGTCCT = 315 reads: +388 validated
umi ACATACACGA = 233 reads: +388 validated
umi ACCCGCCGCT = 268 reads: +388 validated
umi ACGATCCTTT = 305 reads: +388 validated
umi ACGATCGGGG = 354 reads: +388 validated
umi ACGTGAGGAC = 265 reads: +388 validated
umi ACTTCCCATG = 325 reads: +388 validated
umi ATCAGCCTAC = 177 reads: +388 validated
umi ATGTGTCATA = 289 reads: +388 validated
umi ATTCGGCCAG = 278 reads: +388 validated
umi CAATTCGTGT = 254 reads: +388 validated
umi CAGCCCCACA = 338 reads: +388 validated
umi CATTACTCTC = 291 reads: +388 validated
umi CCACGCTATT = 400 reads: +388 validated
umi CCGTTAATGT = 174 reads: +388 validated
umi CGATTAGCGC = 318 reads: +388 validated
umi CGCCAGGTGT = 303 reads: +388 validated
umi CGTCCTCAAC = 107 reads: +388 validated
umi CGTTGAATGG = 276 reads: +388 validated
umi CTAACATCGC = 326 reads: +388 validated
umi CTAATCAAGG = 238 reads: +51 -1 +336 non-validated
umi CTACACGCTA = 313 reads: +388 validated
umi CTCCTTCTCC = 348 reads: +388 validated
umi CTCGCCGCCT = 360 reads: +388 validated
umi CTTGTCCTAC = 316 reads: +388 validated
umi GCACGCCCCG = 288 reads: +388 validated
umi GTACCTTCAT = 312 reads: +388 validated
umi GTATGTGCCT = 161 reads: +388 validated
umi GTATTGACGG = 209 reads: +388 validated
umi GTGCCTACAC = 209 reads: +388 validated
umi GTTCTGTTCC = 362 reads: +388 validated
umi TACTAGTGCA = 358 reads: -142X +246 invalidated
umi TAGTCGGGGT = 255 reads: +388 validated
umi TCATATAATG = 372 reads: +388 validated
umi TCCGCCTCGC = 304 reads: +388 validated
umi TCTGAATTCA = 368 reads: +388 validated
umi TCTGACGACT = 266 reads: +388 validated
umi TGCAACGCCT = 344 reads: +388 validated
umi TTAGTCATAG = 324 reads: +388 validated
umi TTCTATAGCC = 189 reads: +388 validated
umi TTGGAGCATA = 263 reads: +388 validated
umi TTTAGCCCAC = 334 reads: +388 validated
umi TTTCCGAGGG = 303 reads: +388 validated

UMI info for barcode GCTGCTTCAGGACCCT-1 contig 2 = CCACATCCCT...
umi CTCCTATACG = 159 reads: +421 validated
umi CTGTATGCGT = 118 reads: +421 validated
umi GATTGATACT = 172 reads: -382 +1 -1X +1 -1X +8 -2XX +2 -2XX +1 -1XX +8 -1XX +10 invalidated
umi GTACATTAAT = 367 reads: -378 +2 -1XX +2 -1XX +1 -1XX +8 -2XX +2 -2XX +1 -1XX +8 -1XX +10 invalidated
umi TTTCACATGA = 77 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 20-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=16)
377-415 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 44 umis using 2136 reads
cdr3 = CQQYYSPLVTF at 354, score = 9 + 8
umis assigned: [11, 62, 75, 92, 106, 107, 118, 140, 210, 232] and 34 others
of which 44 are surviving nonsolos
reads assigned: 12383
start codons at 27, 33, 89, 102, 238, 457
confident = true

TIG 2[bases=645]
0-42 ==> 0-42 on |72|IGHV1-3|5'UTR| [len=42] (mis=0)
42-395 ==> 0-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=15)
395-411 ==> 0-16 on |25|IGHD4-17|D-REGION| [len=16] (mis=1)
410-463 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=4)
463-645 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 3 umis using 43 reads
cdr3 = CARNDYGDFHWYFDFW at 384, score = 9 + 7
umis assigned: [509, 528, 615, 726, 1083]
of which 5 are surviving nonsolos
reads assigned: 882
start codons at 42, 193, 240, 245, 262, 339, 394
confident = true
now this is a cell
paired!

GAAGACACGGCTGTGTATTTCTGCGCGCGCAATGACTACGGTGACTTCCACTGGTACTTCGATTTCTGGGGCCGTGGCTCACTGGTCACTGTCTCCTCAG <==> ATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGTATTACAGTCCCCTGGTGACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.726 = GCTGCTTCAGTATCTG-1

using 217 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 3^2, 203]
surviving nonsolo ucounts = 1[203]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.730 = GCTGCTTCATAAAGGT-1

using 249 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2^2, 5, 6, 226]
surviving nonsolo ucounts = 1[226]
ids = [2]

====================================================================================

UMI info for barcode GCTGCTTCATAAAGGT-1 contig 1 = GAATCAGTCC...
umi CAAGTTTGTG = 215 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=490]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-490 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.749 = GCTGCTTCATGTAAGA-1

using 354 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 6[2, 3, 4, 5, 8, 317]
surviving nonsolo ucounts = 1[317]
ids = [12]

====================================================================================

UMI info for barcode GCTGCTTCATGTAAGA-1 contig 1 = AGGAACTGCT...
umi CTTTCGTCCC = 317 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
0-32 ==> 65-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
32-377 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=15)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYNLWPPMCSF at 353, score = 9 + 6
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 313
start codons at 32, 101, 174, 237, 380, 413, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.791 = GCTGCTTGTCATGCAT-1

using 315 reads

====================================================================================

graph has 79 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 308]
surviving nonsolo ucounts = 1[308]
ids = [2]

====================================================================================

UMI info for barcode GCTGCTTGTCATGCAT-1 contig 1 = GGGGAGGAAC...
umi CGGTCCAGTG = 307 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=560]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
386-424 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
424-560 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQYNNWPPRETF at 357, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 36, 105, 241, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.792 = GCTGCTTGTCCAGTGC-1

using 213 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^3, 3, 4, 194]
surviving nonsolo ucounts = 1[194]
ids = [10]

====================================================================================

UMI info for barcode GCTGCTTGTCCAGTGC-1 contig 1 = CTGCTTCAGC...
umi TCAGGTTGCC = 191 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=539]
0-48 ==> 3-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
48-404 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
404-442 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
442-539 ==> 0-97 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQSYDSSLSGLYVF at 372, score = 7 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 48, 202, 205, 256, 355, 382, 406
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.803 = GCTGCTTGTCTTCAAG-1

using 1556 reads

====================================================================================

graph has 1855 edges initially, 50 edges after simplification

total ucounts = 554
nonsolo ucounts = 249[2^119, 3^44, 4^33, 5^22, 6^12, 7^3, 8^8, 9^2, 10, 12^2, 17, 195, 218]
surviving nonsolo ucounts = 2[195, 218]
ids = [291, 467]

====================================================================================

UMI info for barcode GCTGCTTGTCTTCAAG-1 contig 1 = GAAGCTCAGC...
umi TCTAGAATGC = 211 reads: +391 validated

UMI info for barcode GCTGCTTGTCTTCAAG-1 contig 2 = AGTCTCAGTC...
umi GACAAAGCGG = 195 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=520]
0-53 ==> 3-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
53-404 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=14)
406-444 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
444-520 ==> 0-76 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CATWDDSLKGPYVF at 374, score = 7 + 8
umis assigned: [467]
of which 1 are surviving nonsolos
reads assigned: 207
start codons at 53, 207, 357, 382, 387, 408
confident = false

TIG 2[bases=544]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=1)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQSYSTPLTF at 347, score = 9 + 9
umis assigned: [291]
of which 1 are surviving nonsolos
reads assigned: 190
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.834 = GCTGCTTGTTTGACTG-1

using 15 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.850 = GCTGCTTTCAGTGTTG-1

using 301 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2, 3, 4^2, 280]
surviving nonsolo ucounts = 1[280]
ids = [4]

====================================================================================

UMI info for barcode GCTGCTTTCAGTGTTG-1 contig 1 = GATCAGGACT...
umi CGTGAATGGG = 268 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=513]
0-30 ==> 0-30 on |266|IGKV2-30|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=0)
387-424 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=4)
424-513 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CMQGTHWPLF at 366, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 264
start codons at 30, 63, 91, 99, 187, 349, 369, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.860 = GCTGCTTTCCACTCCA-1

using 14 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.862 = GCTGCTTTCCATGAAC-1

using 7042 reads

====================================================================================

graph has 4365 edges initially, 62 edges after simplification

total ucounts = 1022
nonsolo ucounts = 505[2^214, 3^126, 4^58, 5^30, 6^21, 7^17, 8^7, 9, 10, 11^2, 13, 16, 19, 23, 26, 29, 53, 57, 83, 140, 141, 149, 158, 168, 176, 199, 202, 223, 238, 240, 241, 251, 256, 259, 261, 268, 291, 815]
surviving nonsolo ucounts = 22[53, 57, 83, 140, 141, 149, 158, 168, 176, 199, 202, 223, 238, 240, 241, 251, 256, 259, 261, 268, 291, 815]
ids = [873, 834, 154, 858, 460, 976, 723, 119, 369, 263, ...]

====================================================================================

UMI info for barcode GCTGCTTTCCATGAAC-1 contig 1 = GCTCTGCTTC...
umi ACCTTGTTCG = 166 reads: +391 validated
umi ACCTTGTTTG = 261 reads: +391 validated
umi ATAATCGTGA = 230 reads: +391 validated
umi CAACGACCTC = 201 reads: +391 validated
umi CCGACTGCAT = 176 reads: +391 validated
umi CGCATTCGTT = 249 reads: +391 validated
umi CTGCGGCACC = 245 reads: +391 validated
umi GTACTCCGGA = 269 reads: +391 validated
umi GTGAGTTGGG = 260 reads: +391 validated
umi TATATGTCCT = 90 reads: +293 -83 +15 non-validated
umi TCTAACTCTA = 224 reads: +391 validated
umi TCTGCTCGAC = 58 reads: +391 validated
umi TCTTTCCCGC = 241 reads: +391 validated
umi TTAACTTTAC = 296 reads: -24X +367 invalidated

UMI info for barcode GCTGCTTTCCATGAAC-1 contig 2 = GGGAGAGGAG...
umi AAATCCCCTT = 816 reads: -239 +188 non-validated
umi AGACACTTTC = 84 reads: +427 validated
umi CACTACCACT = 218 reads: +14 -5X +2 -1XX +405 invalidated
umi CTAGGATGTA = 141 reads: +427 validated
umi GAACTTAACA = 262 reads: +427 validated
umi TGCATTTAGT = 138 reads: +427 validated
umi TGGGGAACAT = 52 reads: +391 -1 +1 -34 non-validated
umi TTGTAGGGTT = 150 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=653]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=11)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
442-653 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 13 umis using 465 reads
cdr3 = CQSYDSSLSGWVF at 375, score = 8 + 8
umis assigned: [119, 120, 194, 263, 369, 412, 497, 630, 653, 723] and 4 others
of which 14 are surviving nonsolos
reads assigned: 2917
start codons at 51, 205, 358, 385
confident = true

TIG 2[bases=660]
0-73 ==> 148-221 on |145|IGHV3-53|5'UTR| [len=221] (mis=0)
73-403 ==> 0-330 on |146|IGHV3-53|L-REGION+V-REGION| [len=350] (mis=9)
465-500 ==> 11-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
500-660 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 7 umis using 241 reads
cdr3 = CTRVVLFGRWESSGYSSVW at 412, score = 8 + 7
umis assigned: [16, 154, 293, 460, 532, 858, 873, 976]
of which 8 are surviving nonsolos
reads assigned: 1819
start codons at 73, 229, 373, 438, 554
confident = true
now this is a cell
paired!

GCCACATATTACTGCACGAGAGTAGTCCTCTTTGGAAGATGGGAAAGTAGTGGTTATTCCTCGGTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGACTCCAGGCTGAGGATGAGGCTGATTACTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.863 = GCTGCTTTCCCACTTG-1

using 20 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.870 = GCTGCTTTCCTCCTAG-1

using 144 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[144]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.874 = GCTGCTTTCGCTTGTC-1

using 396 reads

====================================================================================

graph has 190 edges initially, 6 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[12, 160, 219]
surviving nonsolo ucounts = 2[160, 219]
ids = [5, 7]

====================================================================================

UMI info for barcode GCTGCTTTCGCTTGTC-1 contig 1 = GAATCAGTCC...
umi GCTGGCGTGT = 146 reads: +388 validated

UMI info for barcode GCTGCTTTCGCTTGTC-1 contig 2 = GATCAGGACT...
umi TGTGACTGCA = 199 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=499]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-499 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 145
start codons at 25, 31, 100, 236, 455
confident = false

TIG 2[bases=513]
0-30 ==> 0-30 on |266|IGKV2-30|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=9)
389-427 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
427-513 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CMQGTHWPWTF at 366, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 193
start codons at 30, 63, 91, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.880 = GCTGCTTTCGTTGACA-1

using 11032 reads

====================================================================================

graph has 8524 edges initially, 130 edges after simplification

total ucounts = 2247
nonsolo ucounts = 1031[2^465, 3^230, 4^121, 5^73, 6^46, 7^21, 8^14, 9^12, 10^2, 11^5, 12^2, 13^3, 17, 27^2, 34, 35, 40, 52, 69, 70, 103, 121, 129, 155, 159, 167, 180, 183, 185^2, 197, 201, 204, 209, 212, 214, 216, 220, 221, 222, 248, 249, 261, 263, 342, 354, 380, 414]
surviving nonsolo ucounts = 31[34, 40, 69, 70, 103, 121, 129, 155, 159, 167, 180, 183, 185^2, 197, 201, 204, 209, 212, 214, 216, 220, 221, 248, 249, 261, 263, 342, 354, 380, 414]
ids = [1924, 1904, 899, 848, 1865, 709, 233, 315, 1903, 1943, ...]

====================================================================================

UMI info for barcode GCTGCTTTCGTTGACA-1 contig 1 = GCTGGGGTCT...
umi AAACCCCACA = 246 reads: +388 validated
umi ACACCAGCGC = 210 reads: +388 validated
umi AGAGTACTCC = 257 reads: +388 validated
umi AGATCTTGCG = 156 reads: +388 validated
umi CCTAAGGCAC = 262 reads: +388 validated
umi CGACTAGGGT = 69 reads: +388 validated
umi CGTGCGATCA = 166 reads: +23 -3XX +1 -4XX +1 -2XX +3 -6XX +345 invalidated
umi CGTTAGGGGA = 198 reads: +388 validated
umi CTATGCTGCA = 203 reads: +388 validated
umi GCAGACACCG = 187 reads: +388 validated
umi GGATCCTCTT = 27 reads: -381X +1 -4X +2 invalidated
umi GGTTACTGAG = 200 reads: +388 validated
umi TAACTTTCTT = 205 reads: +388 validated
umi TACGGAAGTG = 121 reads: +15 -2 +1 -3XX +2 -3XX +1 -4XX +1 -2XX +3 -6XX +303 -1 +41 invalidated
umi TAGCACGGCA = 189 reads: +388 validated
umi TCTTGTTGCA = 162 reads: +388 validated
umi TCTTTAATCG = 40 reads: +386 -2 non-validated
umi TGCCGGATTC = 22 reads: -388 non-validated

UMI info for barcode GCTGCTTTCGTTGACA-1 contig 2 = GAGCTCTGGG...
umi ACGGTCCCGC = 129 reads: +388 -2 +4 -19X +1 -1 +2 -2X +2 -4X +1 -2 +3 -1 +1 invalidated
umi ATGTGGGTCC = 140 reads: -382 +1 -5X +1 -1XX +1 -6XX +2 -1XX +11 -1XX +21 invalidated
umi CAATATATCT = 149 reads: -379X +1 -2XX +1 -5XX +1 -1XX +1 -6XX +2 -1XX +11 -1XX +21 invalidated
umi CATTCAGTTT = 53 reads: -379X +1 -2XX +1 -5XX +1 -1XX +1 -6XX +2 -1XX +11 -1XX +21 invalidated
umi CCGAGTGCAC = 104 reads: -379X +1 -2X +1 -5X +1 -1XX +1 -6XX +2 -1XX +11 -1XX +21 invalidated
umi CCTAACTCGT = 32 reads: -379 +1 -2XX +1 -5X +1 -1XX +1 -6X +2 -1XX +11 -1XX +21 invalidated
umi CGGGAATCAA = 222 reads: +433 validated
umi CTGATTGCAT = 330 reads: -387X +2 -2XX +1 -1XX +3 -3XX +8 -1XX +2 -2XX +2 -2XX +17 invalidated
umi TCCCTTTGCA = 50 reads: -127 +1 -1X +18 -3XX +4 -1XX +4 -1XX +22 -1XX +11 -1XX +5 -2XX +2 -2XX +4 -6XX +1 -1XX +1 -1XX +1 -1XX +3 -1XX +1 -3XX +6 -1XX +8 -1XX +32 -3XX +1 -1XX +4 -2XX +1 -2XX +1 -1XX +6 -1XX +6 -80XX +2 -2X +1 -1X +3 -3X +8 -1X +2 -2XX +2 -2XX +17 invalidated
umi TCTAGGTAGG = 102 reads: +433 validated
umi TGACGACGGA = 33 reads: +322 -1 +13 -1 +66 -2 +28 non-validated
umi TGATTGCGGC = 168 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=640]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-375 ==> 0-334 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=9)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
429-640 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 15 umis using 387 reads
cdr3 = CSSFPGSIFGLF at 365, score = 7 + 8
umis assigned: [16, 165, 306, 315, 849, 899, 979, 982, 1031, 1268] and 8 others
of which 18 are surviving nonsolos
reads assigned: 2875
start codons at 41, 198, 249, 348
confident = true

TIG 2[bases=695]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=19)
465-513 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=7)
513-695 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 3 umis using 45 reads
cdr3 = CARDQARYFGELLRRGFDYW at 422, score = 9 + 6
umis assigned: [233, 514, 584, 709, 817, 848, 948, 1075, 1814, 1865] and 2 others
of which 11 are surviving nonsolos
reads assigned: 1480
start codons at 80, 231, 236, 274, 289, 294, 297, 306, 315, 383
confident = true
now this is a cell
paired!

GTCTATTACTGTGCGAGAGATCAGGCTCGATATTTCGGGGAGTTATTAAGGAGGGGATTTGACTATTGGGGCCAGGGAGCCCTGGTCACCGTCTCCTCAG <==> TCTGGGCTCCAGGCTGACGATGAGGCTGATTATTACTGCAGCTCATTTCCAGGAAGCATTTTTGGACTTTTCGGCGGAGGGACCAAACTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.881 = GCTGCTTTCTAACGGT-1

using 8241 reads

====================================================================================

graph has 8302 edges initially, 149 edges after simplification

total ucounts = 2276
nonsolo ucounts = 1113[2^491, 3^272, 4^146, 5^72, 6^42, 7^25, 8^12, 9^13, 10^6, 11^2, 12^4, 13, 21, 23, 25, 36, 42, 50, 51, 55, 64, 66, 69, 90, 128, 130, 133, 134, 149, 150, 178, 187, 190, 241, 245, 261, 263, 285, 287]
surviving nonsolo ucounts = 21[23, 25, 42, 55, 64, 66, 90, 128, 133, 134, 149, 150, 178, 187, 190, 241, 245, 261, 263, 285, 287]
ids = [79, 619, 611, 388, 1865, 62, 808, 78, 662, 1344, ...]

====================================================================================

UMI info for barcode GCTGCTTTCTAACGGT-1 contig 1 = AGGAGTCAGA...
umi AATGTACTGT = 257 reads: +385 validated
umi ACGCCTGATG = 179 reads: +385 validated
umi ACTTTGCGGC = 148 reads: +385 validated
umi CACCGACTCT = 281 reads: +385 validated
umi CCTGGTTTGT = 243 reads: +385 validated
umi CTTCGCTTTT = 263 reads: +385 validated
umi GCTATCTGGT = 137 reads: -8 +377 non-validated
umi TATTCGCACA = 289 reads: +385 validated
umi TATTTACTTC = 238 reads: +385 validated

UMI info for barcode GCTGCTTTCTAACGGT-1 contig 2 = GGGATGCTTT...
umi AACGTATCTT = 64 reads: +417 -1 +7 -14 non-validated
umi AAGACAGGTA = 126 reads: +439 validated
umi ATATAGGAAA = 150 reads: +439 validated
umi CAGCAGCGCT = 26 reads: +339 -51 +49 non-validated
umi CATATACCCG = 130 reads: +439 validated
umi GGTATACAAG = 187 reads: +439 validated
umi TTTTGCGCGG = 189 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=548]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=5)
375-412 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=4)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 9 umis using 328 reads
cdr3 = CHQYNSYSSF at 354, score = 8 + 7
umis assigned: [124, 210, 265, 580, 857, 1153, 1344, 1715, 1723]
of which 9 are surviving nonsolos
reads assigned: 2008
start codons at 27, 33, 89, 102, 334, 454
confident = true

TIG 2[bases=640]
19-396 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=10)
410-458 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
458-640 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 5 umis using 87 reads
cdr3 = CASGGGGDNSFDYW at 385, score = 9 + 7
umis assigned: [62, 78, 400, 619, 662, 1424, 2267]
of which 7 are surviving nonsolos
reads assigned: 865
start codons at 3, 19, 28, 40, 84
confident = true

REJECT CONTIGS

TIG 1[bases=605]
2-354 ==> 0-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=8)
352-403 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
403-605 ==> 0-202 on rc of segment before IGHJ5 exon 1 [len=348] (mis=1)
umis assigned: [79, 388, 611, 808]
of which 4 are surviving nonsolos
reads assigned: 196
start codons at 125, 173, 245, 347, 527, 565
confident = false
did not find CDR3
now this is a cell
paired!

ACCGCCGCAGACACGGCTGTGTATTACTGTGCGTCGGGGGGCGGCGGTGACAACTCATTTGACTACTGGGGCCAGGGAACCGTGGTCACCGTCTCCTCAG <==> ACCATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCATCAGTATAATAGTTATTCTAGTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.884 = GCTGCTTTCTAAGCCA-1

using 318 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[3, 4^3, 8, 9, 280]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode GCTGCTTTCTAAGCCA-1 contig 1 = GGACTCAACA...
umi CCCCAATATG = 250 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=528]
57-410 ==> 0-353 on |736|IGHV1-8|L-REGION+V-REGION| [len=353] (mis=10)
437-487 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=5)
487-528 ==> 0-41 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARGNVVLNWFSIRAFDIW at 399, score = 9 + 8
umis assigned: [3]
of which 0 are surviving nonsolos
reads assigned: 249
start codons at 57, 208, 260, 321, 354, 468, 505
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.895 = GCTGCTTTCTCGCTTG-1

using 265 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[11, 254]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.904 = GCTGCTTTCTGCTGCT-1

using 347 reads

====================================================================================

graph has 208 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 343]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.926 = GCTGGGTAGACACTAA-1

using 279 reads

====================================================================================

graph has 129 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 3, 266]
surviving nonsolo ucounts = 1[266]
ids = [3]

====================================================================================

UMI info for barcode GCTGGGTAGACACTAA-1 contig 1 = TGCTTCAGCT...
umi GCTTTTGCCA = 253 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=510]
0-47 ==> 4-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
47-403 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
403-441 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
441-510 ==> 0-69 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQSYDSSLSGLYVF at 371, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 47, 201, 204, 255, 354, 381, 405
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.927 = GCTGGGTAGACAGACC-1

using 132 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 3, 122]
surviving nonsolo ucounts = 1[122]
ids = [7]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.937 = GCTGGGTAGATCCGAG-1

using 2660 reads

====================================================================================

graph has 3222 edges initially, 48 edges after simplification

total ucounts = 1095
nonsolo ucounts = 507[2^213, 3^128, 4^62, 5^37, 6^21, 7^18, 8^8, 9^8, 10^3, 11^5, 12, 13^2, 317]
surviving nonsolo ucounts = 1[317]
ids = [515]

====================================================================================

UMI info for barcode GCTGGGTAGATCCGAG-1 contig 1 = AGGAGTCAGA...
umi CTCCAATTCT = 302 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-341 ==> 0-314 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=19)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=8)
415-499 ==> 0-84 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQFYLIPYTF at 354, score = 8 + 7
umis assigned: [515]
of which 1 are surviving nonsolos
reads assigned: 292
start codons at 27, 33, 89, 102, 189, 334, 399, 406, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.938 = GCTGGGTAGATCCTGT-1

using 526 reads

====================================================================================

graph has 232 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 8[2^2, 3^3, 4, 221, 280]
surviving nonsolo ucounts = 2[221, 280]
ids = [4, 0]

====================================================================================

UMI info for barcode GCTGGGTAGATCCTGT-1 contig 1 = AGGAATCAGT...
umi AATCCTTTCA = 283 reads: +388 validated
umi CTAGTCTCCT = 222 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 81 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0, 4]
of which 2 are surviving nonsolos
reads assigned: 495
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.951 = GCTGGGTAGCGATTCT-1

using 20763 reads

====================================================================================

graph has 8110 edges initially, 138 edges after simplification

total ucounts = 1119
nonsolo ucounts = 548[2^205, 3^107, 4^78, 5^37, 6^19, 7^9, 8^2, 9^4, 10, 11, 12^3, 13^2, 14, 17, 18, 21, 24, 28, 37, 44, 45, 53, 57, 63, 73, 106, 135, 141, 144, 148, 159, 166, 171, 179, 187, 189, 194, 199, 207, 209, 213, 219, 227, 229, 230, 231^2, 232, 243, 247, 253, 255^4, 256, 257, 258, 260, 261^2, 262, 267, 269, 271^2, 272, 280, 283, 293, 294^2, 296, 302, 307, 308, 312, 313, 321, 323, 324, 330, 333, 343, 348, 378, 399, 412, 416, 521, 568, 586]
surviving nonsolo ucounts = 72[24, 37, 45, 63, 73, 106, 135, 141, 144, 148, 159, 166, 171, 179, 187, 189, 194, 199, 207, 209, 213, 219, 227, 229, 230, 231^2, 232, 243, 247, 253, 255^4, 256, 257, 258, 260, 261^2, 262, 267, 269, 271^2, 272, 280, 283, 293, 294^2, 296, 302, 307, 308, 312, 313, 321, 323, 324, 330, 333, 343, 348, 378, 399, 412, 416, 521, 568, 586]
ids = [7, 320, 920, 864, 835, 370, 655, 1110, 1005, 929, ...]

====================================================================================

UMI info for barcode GCTGGGTAGCGATTCT-1 contig 1 = ACATGGGAAA...
umi AAACCAATCT = 321 reads: -137X +1 -1X +1 -16XX +1 -3XX +1 -3XX +1 -3XX +1 -1XX +1 -1XX +261 invalidated
umi ACCTGGTACA = 333 reads: +433 validated
umi ATTCCGGCTC = 35 reads: +189 -1 +122 -1 +1 -1 +2 -1 +1 -2 +31 -1 +65 -15 non-validated
umi GAATCAGGTT = 206 reads: +433 validated
umi GAGATCTCCA = 253 reads: +413 -20 non-validated
umi GATGGTCTAA = 178 reads: -398X +8 -2X +2 -1X +3 -3X +11 -1XX +2 -1XX +1 invalidated
umi GGAGTATAAG = 171 reads: +427 -6 non-validated
umi TTTCAAACGG = 140 reads: +426 -7 non-validated

UMI info for barcode GCTGGGTAGCGATTCT-1 contig 2 = TACAACAGGC...
umi AACTAATCAC = 279 reads: +400 validated
umi AATTCTCCTC = 257 reads: +400 validated
umi ACCCGCCCGT = 310 reads: +400 validated
umi ACCTACGCAC = 418 reads: +20 -1XX +379 invalidated
umi ACCTATTTGC = 329 reads: +400 validated
umi ACTACAATCA = 174 reads: +400 validated
umi ACTCCTGATT = 181 reads: +400 validated
umi ACTTTATATT = 256 reads: +400 validated
umi AGGTAAGCCA = 318 reads: +400 validated
umi AGGTTGACCG = 250 reads: +400 validated
umi ATAACCTGAC = 280 reads: +400 validated
umi ATCTGTACTT = 305 reads: +400 validated
umi ATGCCCAGTA = 327 reads: +400 validated
umi CCCCGTCACG = 256 reads: +400 validated
umi CTCCTTTCCC = 377 reads: -5X +395 invalidated
umi CTCTTGGGCC = 348 reads: +400 validated
umi CTGCAGGTAT = 187 reads: +400 validated
umi CTTCCTACGG = 189 reads: +387 -2X +1 -2XX +1 -2XX +4 -1XX invalidated
umi GATCATGGAG = 303 reads: +400 validated
umi GCAGACCTGT = 135 reads: +400 validated
umi GCATTATCAG = 209 reads: +400 validated
umi GCCTCCCCTT = 329 reads: +400 validated
umi GCGACATCTA = 304 reads: +400 validated
umi GCGGACTTTA = 193 reads: +400 validated
umi GGGTAATCAG = 261 reads: +400 validated
umi GGGTACTGAC = 260 reads: +400 validated
umi GGGTAGGGGG = 232 reads: +400 validated
umi GTACGGTCTT = 220 reads: +400 validated
umi GTAGCCCCCC = 265 reads: +400 validated
umi GTTCCGCTCA = 324 reads: +400 validated
umi TAACATATAT = 214 reads: +400 validated
umi TAGTCTGGTC = 258 reads: +400 validated
umi TCCACTATCG = 243 reads: +400 validated
umi TCCAGCCACA = 271 reads: +400 validated
umi TCTTTTGGCA = 301 reads: +400 validated
umi TGACACCACT = 296 reads: +400 validated
umi TGGTACTCCT = 253 reads: +400 validated
umi TGTCTTGTTA = 254 reads: +400 validated
umi TTCTGGCTTC = 259 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=550]
0-46 ==> 18-64 on |191|IGHV4-4|5'UTR| [len=64] (mis=3)
46-399 ==> 0-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=22)
430-479 ==> 0-49 on |56|IGHJ5|J-REGION| [len=49] (mis=12)
479-550 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 71 reads
cdr3 = CARDIPGPQASCGADCYTTW at 388, score = 9 + 9
umis assigned: [8, 148, 320, 601, 620, 630, 721, 1110]
of which 8 are surviving nonsolos
reads assigned: 1618
start codons at 2, 25, 46, 90
confident = true

TIG 2[bases=562]
0-26 ==> 149-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
26-389 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=11)
389-426 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
426-562 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 38 umis using 1370 reads
cdr3 = CQQYYSTPLTF at 365, score = 9 + 9
umis assigned: [38, 82, 131, 144, 146, 164, 173, 189, 228, 231] and 29 others
of which 39 are surviving nonsolos
reads assigned: 10237
start codons at 26, 95, 348, 468
confident = true

REJECT CONTIGS

TIG 1[bases=561]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=21)
395-425 ==> 0-30 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
425-561 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [47, 56, 109, 197, 254, 284, 366, 549, 758, 798] and 3 others
of which 13 are surviving nonsolos
reads assigned: 4030
start codons at 30, 99, 260, 352, 467
confident = false
did not find CDR3

TIG 2[bases=471]
3-320 ==> 35-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=5)
318-369 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
369-471 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [506, 920, 929]
of which 3 are surviving nonsolos
reads assigned: 468
start codons at 91, 139, 211, 313, 387, 448
confident = false
did not find CDR3
now this is a cell
paired!

CTTTATTATTGTGCGAGAGACATACCGGGGCCTCAAGCGAGTTGCGGTGCTGACTGCTACACAACCTGGGGCCGGGGAACCCGAGTCACCGTCTCATCCG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAACAGTATTATAGTACTCCGCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCCAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.952 = GCTGGGTAGCGCCTTG-1

using 1044 reads

====================================================================================

graph has 319 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3, 297, 737]
surviving nonsolo ucounts = 2[297, 737]
ids = [9, 6]

====================================================================================

UMI info for barcode GCTGGGTAGCGCCTTG-1 contig 1 = GGGGTCACAA...
umi TGAAAAACTG = 734 reads: -336 +2 -1X +46 invalidated
umi TTAGCAGTAA = 297 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=634]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=26)
385-423 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
423-634 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=2)
junction support: 1 umis using 38 reads
cdr3 = CFSYGNTGRVF at 362, score = 8 + 8
umis assigned: [6, 9]
of which 2 are surviving nonsolos
reads assigned: 1018
start codons at 38, 249, 372, 555
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.965 = GCTGGGTAGGACGAAA-1

using 320 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 6, 305]
surviving nonsolo ucounts = 1[305]
ids = [7]

====================================================================================

UMI info for barcode GCTGGGTAGGACGAAA-1 contig 1 = GCTGTGCTGT...
umi TTCCGTTCAA = 288 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=542]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=1)
387-425 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
425-542 ==> 0-117 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQSADSSGTYGVF at 358, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 43, 104, 173, 191, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.973 = GCTGGGTAGGCCCTTG-1

using 1624 reads

====================================================================================

graph has 1948 edges initially, 28 edges after simplification

total ucounts = 735
nonsolo ucounts = 326[2^128, 3^77, 4^37, 5^28, 6^22, 7^12, 8^10, 9^2, 10^3, 11, 12^2, 14^2, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.974 = GCTGGGTAGGGATACC-1

using 209 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[209]
surviving nonsolo ucounts = 1[209]
ids = [0]

====================================================================================

UMI info for barcode GCTGGGTAGGGATACC-1 contig 1 = AGCATCACAT...
umi CTAGCATCTG = 158 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=489]
0-61 ==> 0-61 on |84|IGHV1-69|5'UTR| [len=61] (mis=0)
61-414 ==> 0-353 on |85|IGHV1-69|L-REGION+V-REGION| [len=353] (mis=0)
417-436 ==> 0-19 on |26|IGHD4-23|D-REGION| [len=19] (mis=2)
442-488 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
junction support: 1 umis using 13 reads
cdr3 = CARDDDYGGNSRGDGDYW at 403, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 154
start codons at 61, 212, 259, 358
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.998 = GCTGGGTCAACTTGAC-1

using 2518 reads

====================================================================================

graph has 3363 edges initially, 44 edges after simplification

total ucounts = 1239
nonsolo ucounts = 523[2^221, 3^134, 4^63, 5^43, 6^22, 7^14, 8^9, 9^8, 10^3, 12, 13^2, 15^2, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1015 = GCTGGGTCACGGTAGA-1

using 261 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 7, 244]
surviving nonsolo ucounts = 1[244]
ids = [5]

====================================================================================

UMI info for barcode GCTGGGTCACGGTAGA-1 contig 1 = AAAAACCACA...
umi CTATACATGT = 226 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=532]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-532 ==> 0-46 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 50, 248, 253, 270, 314, 347
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1025 = GCTGGGTCAGGAATCG-1

using 332 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 4, 5, 317]
surviving nonsolo ucounts = 1[317]
ids = [1]

====================================================================================

UMI info for barcode GCTGGGTCAGGAATCG-1 contig 1 = AGTCTCAGTC...
umi CCAACGCATT = 319 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=23)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=6)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQEGYMTPPAF at 347, score = 9 + 6
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 314
start codons at 20, 26, 82, 95, 362, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1034 = GCTGGGTCAGTGGGAT-1

using 272 reads

====================================================================================

graph has 152 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^3, 3, 4, 86, 170]
surviving nonsolo ucounts = 1[170]
ids = [4]

====================================================================================

UMI info for barcode GCTGGGTCAGTGGGAT-1 contig 1 = GGGGTCTCAG...
umi TACATGCTGC = 159 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=476]
0-38 ==> 125-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
38-392 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=2)
388-426 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
426-476 ==> 0-50 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CSSYAGSNNLVF at 362, score = 8 + 9
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 157
start codons at 38, 239, 246, 345, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1049 = GCTGGGTCATGAGCGA-1

using 112 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 109]
surviving nonsolo ucounts = 1[109]
ids = [0]

====================================================================================

UMI info for barcode GCTGGGTCATGAGCGA-1 contig 1 = GAGGAATCAG...
umi ACATTCTCCA = 94 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=511]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-511 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 92
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1054 = GCTGGGTCATGTAAGA-1

using 249 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^3, 3, 234]
surviving nonsolo ucounts = 1[234]
ids = [2]

====================================================================================

UMI info for barcode GCTGGGTCATGTAAGA-1 contig 1 = GGAGGACAAT...
umi CCCCACGGTG = 235 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=622]
17-373 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
373-411 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
411-622 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQSYDSSLSGLYVF at 341, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 231
start codons at 17, 171, 174, 225, 324, 351, 375, 543
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1055 = GCTGGGTCATTAACCG-1

using 8477 reads

====================================================================================

graph has 3951 edges initially, 22 edges after simplification

total ucounts = 643
nonsolo ucounts = 297[2^123, 3^66, 4^29, 5^21, 6^9, 7^10, 8, 9^7, 11, 12^2, 46, 55, 91, 104, 175, 225, 226, 245, 251^2, 253, 263, 270, 274, 288, 291, 294, 296, 302, 303, 315, 319, 322, 338, 340, 346, 370, 383]
surviving nonsolo ucounts = 26[55, 91, 104, 175, 225, 226, 245, 251^2, 253, 263, 274, 288, 291, 294, 296, 302, 303, 315, 319, 322, 338, 340, 346, 370, 383]
ids = [108, 537, 11, 242, 579, 384, 417, 247, 413, 461, ...]

====================================================================================

UMI info for barcode GCTGGGTCATTAACCG-1 contig 1 = AGGAGTCAGA...
umi AATTCTGTTT = 295 reads: +394 validated
umi ACTAACTCCT = 375 reads: +394 validated
umi AGACATTATT = 323 reads: +56 -1XX +1 -1XX +2 -1XX +1 -5XX +4 -20XX +1 -3XX +3 -11XX +284 invalidated
umi AGTTTTGTGA = 50 reads: +372 -22 non-validated
umi CCGAGCACCT = 292 reads: +394 validated
umi CCTTGCTATC = 175 reads: +394 validated
umi GCTTTCTCAG = 227 reads: +394 validated
umi GGATAGCTCG = 274 reads: +394 validated
umi GTACGATCGC = 250 reads: +394 validated
umi GTATCTCTTT = 244 reads: +394 validated
umi GTATTAGCTT = 392 reads: +56 -1XX +1 -1XX +2 -1XX +1 -5XX +4 -20XX +1 -3XX +3 -11XX +284 invalidated
umi TAAGAGTTTG = 303 reads: +394 validated
umi TACCTTATCG = 250 reads: +394 validated
umi TAGACCGCTA = 319 reads: +394 validated
umi TCAGCTTGGT = 263 reads: +394 validated
umi TCCAGTGCGT = 349 reads: +394 validated
umi TCTGGCTACA = 91 reads: +394 validated
umi TGACTTTCTC = 293 reads: +394 validated
umi TGTTACATCT = 336 reads: +394 validated
umi TTAACACTAT = 337 reads: +394 validated
umi TTACATATGC = 301 reads: +394 validated
umi TTACCATCAA = 225 reads: +394 validated
umi TTCCCTCCCT = 296 reads: +394 validated

UMI info for barcode GCTGGGTCATTAACCG-1 contig 2 = AGCTCTGAGA...
umi AAGACTACCC = 103 reads: +418 validated
umi CGAGTCAACA = 255 reads: +418 validated
umi GTACCCGAGA = 280 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=557]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=5)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 22 umis using 1004 reads
cdr3 = CQQYNSYSRMYTF at 354, score = 8 + 8
umis assigned: [24, 64, 81, 108, 216, 242, 384, 390, 413, 417] and 13 others
of which 23 are surviving nonsolos
reads assigned: 6146
start codons at 27, 33, 89, 102, 238, 241, 334, 381, 463
confident = true

TIG 2[bases=568]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=3)
444-497 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=2)
497-568 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 55 reads
cdr3 = CAKPPMLHSWYFDLW at 421, score = 9 + 7
umis assigned: [11, 247, 412]
of which 3 are surviving nonsolos
reads assigned: 621
start codons at 79, 230, 235, 382, 436
confident = true
now this is a cell
paired!

GCCGAGGACACGGCCGTATATTACTGTGCGAAGCCCCCTATGCTTCATTCCTGGTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG <==> AGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAGTTATTCTCGGATGTACACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1057 = GCTGGGTCATTAGGCT-1

using 191 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[190]
surviving nonsolo ucounts = 1[190]
ids = [1]

====================================================================================

UMI info for barcode GCTGGGTCATTAGGCT-1 contig 1 = GGGGTCTCAG...
umi TACGTTAGGG = 184 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=477]
0-38 ==> 3-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=3)
38-382 ==> 0-344 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=20)
388-426 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
426-477 ==> 0-51 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CSSYISSTTLGF at 362, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 181
start codons at 38, 246, 249
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1091 = GCTGGGTGTGCAGGTA-1

using 165 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 3, 4, 5, 150]
surviving nonsolo ucounts = 1[150]
ids = [0]

====================================================================================

UMI info for barcode GCTGGGTGTGCAGGTA-1 contig 1 = GGGAGAGGAG...
umi AGAGTCACGT = 146 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=531]
0-74 ==> 6-80 on |139|IGHV3-43|5'UTR| [len=80] (mis=5)
74-429 ==> 0-355 on |140|IGHV3-43|L-REGION+V-REGION| [len=355] (mis=51)
429-449 ==> 0-20 on |30|IGHD5-24|D-REGION| [len=20] (mis=3)
461-510 ==> 14-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
510-531 ==> 0-21 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CAKDRRDGYIYSPGYYGLDVW at 416, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 142
start codons at 74, 225, 309, 377, 435
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1104 = GCTGGGTGTTACGTCA-1

using 279 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 3, 4, 268]
surviving nonsolo ucounts = 1[268]
ids = [3]

====================================================================================

UMI info for barcode GCTGGGTGTTACGTCA-1 contig 1 = GGGGAGGAAT...
umi TCGGGCCTCT = 239 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=472]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-472 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1110 = GCTGGGTGTTCGTTGA-1

using 320 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 6, 309]
surviving nonsolo ucounts = 1[309]
ids = [1]

====================================================================================

UMI info for barcode GCTGGGTGTTCGTTGA-1 contig 1 = GAGTCAGACT...
umi CATCGGTTGG = 311 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CQQYDSFSWTF at 352, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 25, 31, 87, 100, 236, 239, 332, 362, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1113 = GCTGGGTTCAAACCGT-1

using 252 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^3, 4, 9, 228]
surviving nonsolo ucounts = 1[228]
ids = [2]

====================================================================================

UMI info for barcode GCTGGGTTCAAACCGT-1 contig 1 = GAGAGAGGAG...
umi CCCATTTTGT = 215 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=598]
0-73 ==> 6-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=11)
457-506 ==> 0-49 on |56|IGHJ5|J-REGION| [len=49] (mis=5)
506-598 ==> 0-92 on |3|IGHA2|C-REGION| [len=1019] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CAKDTRRQLLVRVRGWLDPW at 415, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 211
start codons at 73, 229, 376, 406, 560, 579
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1122 = GCTGGGTTCAGAGACG-1

using 281 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 271]
surviving nonsolo ucounts = 1[271]
ids = [4]

====================================================================================

UMI info for barcode GCTGGGTTCAGAGACG-1 contig 1 = GATCAGGACT...
umi CGACACATCC = 259 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=522]
0-30 ==> 0-30 on |266|IGKV2-30|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=8)
389-427 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
427-522 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CMQGTHWPWTF at 366, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 256
start codons at 30, 63, 91, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1127 = GCTGGGTTCAGCGACC-1

using 334 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[3^2, 4, 5, 8, 309]
surviving nonsolo ucounts = 1[309]
ids = [1]

====================================================================================

UMI info for barcode GCTGGGTTCAGCGACC-1 contig 1 = GAGCTACAAC...
umi CATTTCTGGC = 310 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=2)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYYSTLTF at 369, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 306
start codons at 30, 99, 352, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1133 = GCTGGGTTCATGCAAC-1

using 64 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 12[2^4, 3^2, 4, 5, 6^2, 7, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1139 = GCTGGGTTCCACTGGG-1

using 657 reads

====================================================================================

graph has 198 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[655]
surviving nonsolo ucounts = 1[655]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=430]
0-193 ==> 160-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=13)
222-270 ==> 13-61 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
270-430 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
cdr3 = CARGDRRAAAFYYHYMDVW at 182, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 649
start codons at 38, 104, 137, 227, 324
confident = false
VJ delta = 21
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1142 = GCTGGGTTCCAGTATG-1

using 301 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 294]
surviving nonsolo ucounts = 1[294]
ids = [2]

====================================================================================

UMI info for barcode GCTGGGTTCCAGTATG-1 contig 1 = GTCAGACTCA...
umi GTTTCCTCTA = 294 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CQQYDSFSWTF at 350, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 291
start codons at 23, 29, 85, 98, 234, 237, 330, 360, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1151 = GCTGGGTTCCTGTACC-1

using 657 reads

====================================================================================

graph has 216 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 650]
surviving nonsolo ucounts = 1[650]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=508]
0-335 ==> 16-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
334-372 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
372-508 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CLQYSSSPWTF at 311, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 632
start codons at 59, 195, 414
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1156 = GCTGGGTTCGCATGGC-1

using 455 reads

====================================================================================

graph has 196 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[2^2, 4, 198, 246]
surviving nonsolo ucounts = 2[198, 246]
ids = [3, 1]

====================================================================================

UMI info for barcode GCTGGGTTCGCATGGC-1 contig 1 = AGTGACTCCT...
umi CCTTTGTCTC = 185 reads: +433 validated

UMI info for barcode GCTGGGTTCGCATGGC-1 contig 2 = ACACCCTGTG...
umi ATTCAATATA = 236 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=477]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=19)
403-453 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
453-477 ==> 0-24 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 365, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 183
start codons at 20, 64, 243, 246, 249, 335, 405
confident = false

TIG 2[bases=521]
0-38 ==> 9-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
38-389 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=1)
388-426 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
426-521 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQANSFPWTF at 365, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 38, 44, 100, 113, 249, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1159 = GCTGGGTTCGGCGCAT-1

using 266 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 259]
surviving nonsolo ucounts = 1[259]
ids = [6]

====================================================================================

UMI info for barcode GCTGGGTTCGGCGCAT-1 contig 1 = CAGTCCCAGT...
umi TTCGTAGCTT = 237 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=495]
0-21 ==> 37-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
21-372 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=1)
372-409 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
409-495 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQLNSYPLTF at 348, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 21, 27, 83, 232, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1170 = GCTGGGTTCTCGCATC-1

using 4790 reads

====================================================================================

graph has 4030 edges initially, 91 edges after simplification

total ucounts = 1276
nonsolo ucounts = 582[2^268, 3^135, 4^79, 5^35, 6^19, 7^11, 8^8, 9^5, 10^3, 11, 12, 14^2, 15, 18, 20, 58, 107, 149, 162, 174, 185, 194, 204, 205, 242, 256, 294]
surviving nonsolo ucounts = 12[58, 107, 149, 162, 174, 185, 194, 204, 205, 242, 256, 294]
ids = [1080, 1248, 745, 1145, 404, 861, 507, 289, 847, 912, ...]

====================================================================================

UMI info for barcode GCTGGGTTCTCGCATC-1 contig 1 = TGAGCGCAGA...
umi GTCGATCCCT = 201 reads: +385 validated
umi GTGTATGCCA = 142 reads: -355X +16 -2XX +11 -1XX invalidated
umi TACCCGCTAC = 245 reads: +385 validated
umi TTTCCATTCC = 106 reads: -1 +384 non-validated

UMI info for barcode GCTGGGTTCTCGCATC-1 contig 2 = GAGCTCTGGG...
umi ATGCAGTCTG = 192 reads: +430 validated
umi CATGGTGTCA = 173 reads: +430 validated
umi GCGTGGCGGC = 145 reads: +430 validated
umi TGAGCATCAC = 57 reads: +327 -103 non-validated
umi TGCTGAACTA = 242 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=632]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-372 ==> 0-336 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=14)
383-421 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
421-632 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 3 umis using 87 reads
cdr3 = CGTWDDTLNVVF at 357, score = 7 + 8
umis assigned: [847, 861, 912, 1248]
of which 4 are surviving nonsolos
reads assigned: 674
start codons at 36, 190, 241, 365, 370, 382
confident = true

TIG 2[bases=588]
81-399 ==> 0-318 on |116|IGHV3-20|L-REGION+V-REGION| [len=353] (mis=46)
463-511 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
511-588 ==> 0-77 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 4 umis using 74 reads
cdr3 = CARDRQLYYYDRSATFDYW at 423, score = 9 + 7
umis assigned: [289, 404, 745, 1080, 1112]
of which 5 are surviving nonsolos
reads assigned: 797
start codons at 81, 226, 232, 237, 307, 316, 384, 565
confident = true
>vscore_71.1170_84.7%
ATGGAGTTGGGACTGAGCTGGATTTTCCTTTTGGCTATTTTAAAAGGTGTCCAGTGTGAGGTGAAATTGGTGGAGTCTGGGGGAGCCTCGATACAGCCTGGCAGGTCCCTGAAACTCTCCTGTGAAGCCGCTGGATTCGACTTTGATGATTATGCCATGCACTGGGTCCGGCTAACTCCAGGGAAGGGCCTGGAGTGGCTCTCAGGTGTTAAGTGGAATAGTGGTGATGTAGCCTATGCGGACTCTGTGAAGGGCCGATTCACCATCTCGAGAGACAACGCCAAGCACTCCCTGTCTCTGCAAATGAACAATCTGAGACCTGAGGACACGGCATTATATTTTTGTGCAAGAGA
now this is a cell
paired!

GCATTATATTTTTGTGCAAGAGATCGTCAACTATATTACTACGATAGAAGTGCGACGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ACCGGGCTCCAGACTGGGGACGAGGCCATTTATTACTGCGGAACATGGGATGACACCCTGAATGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1182 = GCTGGGTTCTTATCTG-1

using 33 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1184 = GCTGGGTTCTTGACGA-1

using 79 reads

====================================================================================

graph has 80 edges initially, 4 edges after simplification

total ucounts = 50
nonsolo ucounts = 17[2^11, 3^3, 4^2, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1185 = GCTGGGTTCTTGCCGT-1

using 309 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[304]
surviving nonsolo ucounts = 1[304]
ids = [3]

====================================================================================

UMI info for barcode GCTGGGTTCTTGCCGT-1 contig 1 = GGGGTCTCAG...
umi GGATAAGGGA = 306 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=637]
0-38 ==> 3-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=0)
38-361 ==> 0-323 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=12)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
426-637 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CNSYKRDSAWVF at 362, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 38, 189, 339
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1193 = GCTTCCAAGATGCCAG-1

using 479 reads

====================================================================================

graph has 180 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[4, 5^2, 6, 7, 194, 253]
surviving nonsolo ucounts = 2[194, 253]
ids = [11, 6]

====================================================================================

UMI info for barcode GCTTCCAAGATGCCAG-1 contig 1 = AGCTTCAGCT...
umi TAGAACACCA = 249 reads: +388 validated
umi TTGTCGAACC = 24 reads: -339X +2 -1XX +1 -4XX +1 -2XX +1 -2XX +3 -1XX +2 -1XX +5 -1XX +21 -1XX invalidated

GOOD CONTIGS

TIG 1[bases=555]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-555 ==> 0-120 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [6, 11]
of which 2 are surviving nonsolos
reads assigned: 264
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1198 = GCTTCCAAGCCACTAT-1

using 336 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 8[2^2, 3^2, 4^2, 5, 307]
surviving nonsolo ucounts = 1[307]
ids = [4]

====================================================================================

UMI info for barcode GCTTCCAAGCCACTAT-1 contig 1 = GATCAGGACT...
umi CTTGCGACTG = 287 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=508]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-508 ==> 0-78 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 284
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1200 = GCTTCCAAGCCTATGT-1

using 581 reads

====================================================================================

graph has 268 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3^2, 223, 346]
surviving nonsolo ucounts = 2[223, 346]
ids = [6, 5]

====================================================================================

UMI info for barcode GCTTCCAAGCCTATGT-1 contig 1 = GAAGAGCTGC...
umi GGCAGATGTA = 343 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=554]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=6)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQHATSPFTF at 357, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 339
start codons at 33, 241, 367, 460
confident = false

REJECT CONTIGS

TIG 1[bases=452]
1-99 ==> 5512-5610 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=3)
52-412 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=11)
413-438 ==> 0-25 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
cdr3 = CMQGSFWPPYSF at 388, score = 8 + 5
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 52, 85, 121, 209, 371, 391
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1202 = GCTTCCAAGCTCCTCT-1

using 352 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^4, 5, 6, 326]
surviving nonsolo ucounts = 1[326]
ids = [0]

====================================================================================

UMI info for barcode GCTTCCAAGCTCCTCT-1 contig 1 = GAGTCAGTCT...
umi ATTTTATTAG = 305 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=490]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=21)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=6)
413-490 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQSYIMPISF at 352, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 302
start codons at 25, 31, 87, 100, 236, 370, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1204 = GCTTCCAAGGAATCGC-1

using 306 reads

====================================================================================

graph has 198 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^2, 3, 4^2, 5^2, 274]
surviving nonsolo ucounts = 1[274]
ids = [9]

====================================================================================

UMI info for barcode GCTTCCAAGGAATCGC-1 contig 1 = TGCTCAGTTA...
umi GCTCCCACTA = 276 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=544]
0-26 ==> 71-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
26-371 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=3)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=1)
junction support: 1 umis using 54 reads
cdr3 = CQQYNNWPRTF at 347, score = 9 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 272
start codons at 26, 95, 231, 450, 477
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1210 = GCTTCCAAGGTACTCT-1

using 390 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[384]
surviving nonsolo ucounts = 1[384]
ids = [3]

====================================================================================

UMI info for barcode GCTTCCAAGGTACTCT-1 contig 1 = GGGAGGAATC...
umi TAGGTCGGCC = 385 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=554]
30-381 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 72 reads
cdr3 = CLQYSSSPWTF at 357, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 379
start codons at 30, 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1211 = GCTTCCAAGGTCGGAT-1

using 164 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 157]
surviving nonsolo ucounts = 1[157]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1217 = GCTTCCAAGTGACTCT-1

using 198 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 7[2^3, 3, 4, 6, 172]
surviving nonsolo ucounts = 1[172]
ids = [3]

====================================================================================

UMI info for barcode GCTTCCAAGTGACTCT-1 contig 1 = GAGCTACAAC...
umi CTGGGCTCTA = 173 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=3)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQQYYSTPLTF at 369, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 169
start codons at 30, 99, 352, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1219 = GCTTCCAAGTTATCGC-1

using 100 reads

====================================================================================

graph has 148 edges initially, 4 edges after simplification

total ucounts = 22
nonsolo ucounts = 11[2, 3^3, 4^2, 5, 6, 11, 13, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1223 = GCTTCCACAACTGCGC-1

using 998 reads

====================================================================================

graph has 382 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 8[3^2, 4, 6, 7, 13, 275, 684]
surviving nonsolo ucounts = 2[275, 684]
ids = [5, 9]

====================================================================================

UMI info for barcode GCTTCCACAACTGCGC-1 contig 1 = GGAGCCCCAG...
umi CACGAGTGTC = 266 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=571]
0-68 ==> 168-236 on |168|IGHV3-74|5'UTR| [len=236] (mis=0)
68-399 ==> 0-331 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=17)
440-477 ==> 26-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
477-571 ==> 0-94 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CTNLYHFGSEVW at 410, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 68, 224, 285, 531
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1235 = GCTTCCACACCAGTTA-1

using 333 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 8[2^4, 3, 5, 8, 300]
surviving nonsolo ucounts = 1[300]
ids = [12]

====================================================================================

UMI info for barcode GCTTCCACACCAGTTA-1 contig 1 = GGGGAGGAGT...
umi GCTCAGCAGA = 304 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=552]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYDNLLTF at 358, score = 9 + 9
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 298
start codons at 31, 37, 93, 106, 245, 368, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1249 = GCTTCCACAGGACCCT-1

using 248 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 3, 5, 6, 226]
surviving nonsolo ucounts = 1[226]
ids = [10]

====================================================================================

UMI info for barcode GCTTCCACAGGACCCT-1 contig 1 = AGCTTCAGCT...
umi TACAACTGCC = 211 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=577]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-577 ==> 0-142 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 205
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1253 = GCTTCCACAGTGGGAT-1

using 317 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^2, 4^2, 299]
surviving nonsolo ucounts = 1[299]
ids = [6]

====================================================================================

UMI info for barcode GCTTCCACAGTGGGAT-1 contig 1 = GATCAGGACT...
umi CGTTCACAAC = 295 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=2)
389-427 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CMQALQTRETF at 366, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 292
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1258 = GCTTCCACATCCGTGG-1

using 253 reads

====================================================================================

graph has 178 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[253]
surviving nonsolo ucounts = 1[253]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=441]
10-203 ==> 106-299 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=11)
280-328 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
328-441 ==> 0-113 on |40|IGHG1|C-REGION| [len=884] (mis=1)
cdr3 = CVRGGRSLWFGDLLDYW at 246, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 176
start codons at 60, 118, 121, 269
confident = false
VJ delta = 6
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1266 = GCTTCCACATTGCGGC-1

using 92 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 13[2, 3^4, 5, 6, 7, 9^2, 10^2, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1269 = GCTTCCAGTAAATGAC-1

using 405 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2^3, 3^2, 5, 6, 378]
surviving nonsolo ucounts = 1[378]
ids = [0]

====================================================================================

UMI info for barcode GCTTCCAGTAAATGAC-1 contig 1 = GAAGAGCTGC...
umi AGGACTCGTA = 358 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=510]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=5)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-510 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 66 reads
cdr3 = CQQYGSTRTF at 357, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 353
start codons at 33, 241, 367, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1280 = GCTTCCAGTCGGCATC-1

using 210 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[207]
surviving nonsolo ucounts = 1[207]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1283 = GCTTCCAGTCTCACCT-1

using 225 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[225]
surviving nonsolo ucounts = 1[225]
ids = [0]

====================================================================================

UMI info for barcode GCTTCCAGTCTCACCT-1 contig 1 = ACATGGGAAG...
umi GCTTGCATAT = 223 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=575]
46-183 ==> 0-137 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=25)
183-309 ==> 140-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=30)
430-476 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=12)
476-575 ==> 0-99 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CARGPLVRGILGKGYYLDLW at 385, score = 8 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 218
start codons at 2, 46, 90, 355
confident = false
see deletion of 3 bases at pos 137 on |197|IGHV4/OR15-8|L-REGION+V-REGION|
>vscore_71.1283_77.1%
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTCCTGTCGCAGGGACAGCTGCGAGAGTGGGGCGGAGGCCTGTTGGAGACCTCGCAGACGCTGTCACTCACCTGTTCTGTTTACGGTCGAGTCTTCGCTGGCTCCTTCTGGACTTGGATCCGCCAATCCCCAGGCAAGGGGCCGGAGTGGATTGGAGAAATTGCTCAGGGTGGAAGACCCAACTACAACCCGGCCCTCCAGAGTCGCGTCACCATTTCAACTGACACGTCGCAGATCCAGTTCTCCCTGACTGTGAGGTCTATGACCGCCGCGGACTCGGCTACATATTACTGTGCCAGGGG
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1286 = GCTTCCAGTCTTGTCC-1

using 335 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 35
nonsolo ucounts = 14[2^9, 4^3, 50, 234]
surviving nonsolo ucounts = 2[50, 234]
ids = [31, 4]

====================================================================================

UMI info for barcode GCTTCCAGTCTTGTCC-1 contig 1 = GGGGAGGAAT...
umi ACTTTACCTG = 236 reads: +388 validated
umi TCGGTCCCCG = 49 reads: -14 +374 non-validated

GOOD CONTIGS

TIG 1[bases=555]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 46 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [4, 31]
of which 2 are surviving nonsolos
reads assigned: 280
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1290 = GCTTCCAGTGCAACTT-1

using 269 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 9[2^4, 3^4, 240]
surviving nonsolo ucounts = 1[240]
ids = [4]

====================================================================================

UMI info for barcode GCTTCCAGTGCAACTT-1 contig 1 = ACTTTCTGAG...
umi ATCTCAACAG = 232 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=571]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=17)
400-450 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
450-571 ==> 0-121 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 32 reads
cdr3 = CARDFPGNYFTFDIW at 374, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 228
start codons at 35, 79, 159, 229, 431
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1291 = GCTTCCAGTGGTTTCA-1

using 188 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 8[2^3, 3, 4^2, 5, 153]
surviving nonsolo ucounts = 1[153]
ids = [10]

====================================================================================

UMI info for barcode GCTTCCAGTGGTTTCA-1 contig 1 = AGCTCTGAGA...
umi GCATTTTATC = 146 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=510]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
junction support: 1 umis using 12 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 145
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1320 = GCTTCCATCCCGGATG-1

using 61 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 8[2, 3^5, 5, 29]
surviving nonsolo ucounts = 1[29]
ids = [10]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1323 = GCTTCCATCCGCGTTT-1

using 201 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 5[2^2, 3, 5, 178]
surviving nonsolo ucounts = 1[178]
ids = [13]

====================================================================================

UMI info for barcode GCTTCCATCCGCGTTT-1 contig 1 = GGGGGGGGTC...
umi TCTACCAGCC = 175 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=539]
0-42 ==> 0-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=3)
42-400 ==> 0-358 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=5)
395-433 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
433-539 ==> 0-106 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CCSYAGSYTSVVF at 366, score = 8 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 174
start codons at 42, 181, 199, 243, 250, 253, 349, 376
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1329 = GCTTCCATCGGCGGTT-1

using 1241 reads

====================================================================================

graph has 302 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 4^2, 1225]
surviving nonsolo ucounts = 1[1225]
ids = [8]

====================================================================================

REJECT CONTIGS

TIG 1[bases=483]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-68 ==> 0-21 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=0)
73-237 ==> 189-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=8) [SHIFT!]
234-272 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
272-483 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CASWDDSLRGRVF at 205, score = 8 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 1212
start codons at 47, 188, 213, 218
confident = false
not full
frameshifted full length stopped transcript of length 483
VJ delta = 185
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1338 = GCTTCCATCTTTAGTC-1

using 346 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 17
nonsolo ucounts = 9[2^5, 4^2, 5, 315]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode GCTTCCATCTTTAGTC-1 contig 1 = ATACTTTCTG...
umi CCCATCCAGC = 309 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=523]
37-385 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=11)
414-464 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
464-523 ==> 0-59 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CARDLLWFGETRAFDIW at 382, score = 9 + 8
umis assigned: [8]
of which 0 are surviving nonsolos
reads assigned: 303
start codons at 37, 81, 399, 445
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1340 = GCTTGAAAGAAAGTGG-1

using 257 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 7, 243]
surviving nonsolo ucounts = 1[243]
ids = [1]

====================================================================================

UMI info for barcode GCTTGAAAGAAAGTGG-1 contig 1 = AGGAGTCAGA...
umi ATTGTTGTCT = 231 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=476]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
415-476 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQYNNSPWTF at 354, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 228
start codons at 27, 33, 89, 102, 334, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1345 = GCTTGAAAGACTTTCG-1

using 632 reads

====================================================================================

graph has 226 edges initially, 12 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[303, 324]
surviving nonsolo ucounts = 2[303, 324]
ids = [3, 5]

====================================================================================

UMI info for barcode GCTTGAAAGACTTTCG-1 contig 1 = GATCAGGACT...
umi TAAGGATGGT = 324 reads: +397 validated

UMI info for barcode GCTTGAAAGACTTTCG-1 contig 2 = GGGGAGGAGT...
umi ACTTTTTTTA = 306 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=2)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CMQALQTPYTF at 366, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false

TIG 2[bases=558]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
384-422 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
422-558 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYDNLPPITF at 358, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 302
start codons at 31, 37, 93, 106, 245, 368, 464
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1352 = GCTTGAAAGCGCTCCA-1

using 136 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 129]
surviving nonsolo ucounts = 1[129]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1355 = GCTTGAAAGCTTTGGT-1

using 204 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[201]
surviving nonsolo ucounts = 1[201]
ids = [1]

====================================================================================

UMI info for barcode GCTTGAAAGCTTTGGT-1 contig 1 = GGGGAGGAAT...
umi AGTACCTCGA = 192 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=498]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-498 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1359 = GCTTGAAAGGCCCGTT-1

using 22 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1360 = GCTTGAAAGGTGCAAC-1

using 1199 reads

====================================================================================

graph has 292 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 283, 907]
surviving nonsolo ucounts = 2[283, 907]
ids = [2, 9]

====================================================================================

UMI info for barcode GCTTGAAAGGTGCAAC-1 contig 1 = GGGGGTAAGA...
umi AGTTTGGAAT = 264 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=592]
0-23 ==> 5-28 on |380|IGLV5-45|5'UTR| [len=28] (mis=0)
23-392 ==> 0-369 on |381|IGLV5-45|L-REGION+V-REGION| [len=369] (mis=3)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
426-592 ==> 0-166 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CMIWHSSAWVF at 365, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 261
start codons at 23, 165, 297, 309, 348, 368
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1366 = GCTTGAAAGTGTCCAT-1

using 252 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^3, 7, 234]
surviving nonsolo ucounts = 1[234]
ids = [5]

====================================================================================

UMI info for barcode GCTTGAAAGTGTCCAT-1 contig 1 = TGAGCGCAGA...
umi TCGTGGGGAA = 232 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=568]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=12)
389-427 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
427-568 ==> 0-141 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CATWETSLSAPYVF at 357, score = 6 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 227
start codons at 36, 190, 365, 391, 559
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1373 = GCTTGAACAATCACAC-1

using 220 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[8, 207]
surviving nonsolo ucounts = 1[207]
ids = [4]

====================================================================================

UMI info for barcode GCTTGAACAATCACAC-1 contig 1 = GTCAGACCCA...
umi CGTACCGTGG = 196 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=500]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=0)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
414-500 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYNSYSPITF at 350, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 194
start codons at 23, 29, 85, 98, 330, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1378 = GCTTGAACACCTGGTG-1

using 248 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[243]
surviving nonsolo ucounts = 1[243]
ids = [4]

====================================================================================

UMI info for barcode GCTTGAACACCTGGTG-1 contig 1 = AGCTGTGGGC...
umi TAAGTGAGCG = 245 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=630]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=16)
381-419 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=6)
419-630 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CNSRARTGDHVF at 355, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 242
start codons at 40, 122, 159, 188, 235, 239, 338, 383, 551
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1381 = GCTTGAACACTGTTAG-1

using 230 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 4, 6, 211]
surviving nonsolo ucounts = 1[211]
ids = [10]

====================================================================================

UMI info for barcode GCTTGAACACTGTTAG-1 contig 1 = AGGGAACCAT...
umi TTGATCCCGG = 210 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=526]
8-344 ==> 0-336 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
353-390 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
390-526 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CQQYRNSITF at 332, score = 9 + 8
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 8, 432
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1391 = GCTTGAACAGGCAGTA-1

using 798 reads

====================================================================================

graph has 840 edges initially, 10 edges after simplification

total ucounts = 235
nonsolo ucounts = 99[2^38, 3^21, 4^11, 5^10, 6^7, 7^3, 8^2, 11^2, 12^2, 13^2, 278]
surviving nonsolo ucounts = 1[278]
ids = [52]

====================================================================================

UMI info for barcode GCTTGAACAGGCAGTA-1 contig 1 = TGGGGACCCA...
umi ATCCCAACTC = 261 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=549]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=6)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
461-495 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
495-549 ==> 0-54 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 401, score = 7 + 7
umis assigned: [52]
of which 1 are surviving nonsolos
reads assigned: 259
start codons at 59, 257, 262, 279, 323, 356
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1396 = GCTTGAACATATGCTG-1

using 271 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 262]
surviving nonsolo ucounts = 1[262]
ids = [6]

====================================================================================

UMI info for barcode GCTTGAACATATGCTG-1 contig 1 = GAGGAATCAG...
umi TGTGGTAAGG = 264 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=7)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQHKNYPWTF at 355, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 260
start codons at 28, 34, 103, 185, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1398 = GCTTGAACATCCTTGC-1

using 1513 reads

====================================================================================

graph has 2254 edges initially, 18 edges after simplification

total ucounts = 668
nonsolo ucounts = 317[2^137, 3^65, 4^42, 5^26, 6^20, 7^5, 8^8, 9^5, 10^2, 11, 12^2, 15, 18, 19, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1422 = GCTTGAAGTCTCTCTG-1

using 247 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[11, 233]
surviving nonsolo ucounts = 1[233]
ids = [3]

====================================================================================

UMI info for barcode GCTTGAAGTCTCTCTG-1 contig 1 = AGCTCAGCTT...
umi TTTATGCTGC = 230 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=548]
0-52 ==> 62-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
52-405 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
402-440 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
440-548 ==> 0-108 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CASWDDSLRGRVF at 373, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 225
start codons at 52, 206, 356, 381, 386
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1424 = GCTTGAAGTGAGGCTA-1

using 310 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 303]
surviving nonsolo ucounts = 1[303]
ids = [3]

====================================================================================

UMI info for barcode GCTTGAAGTGAGGCTA-1 contig 1 = GGAGCCCCAG...
umi GGTGCGGCTT = 294 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=519]
0-68 ==> 168-236 on |168|IGHV3-74|5'UTR| [len=236] (mis=0)
68-399 ==> 0-331 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=17)
440-477 ==> 26-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
477-519 ==> 0-42 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CTNLYHFGSEVW at 410, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 68, 224, 285
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1426 = GCTTGAAGTGATGTCT-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1428 = GCTTGAAGTGCGATAG-1

using 878 reads

====================================================================================

graph has 294 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 164, 301, 405]
surviving nonsolo ucounts = 3[164, 301, 405]
ids = [4, 5, 3]

====================================================================================

UMI info for barcode GCTTGAAGTGCGATAG-1 contig 1 = GCTCTGCTTC...
umi ATCGTCCCAG = 404 reads: +394 validated
umi CCCACTTGTT = 152 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=608]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-608 ==> 0-163 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 99 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [3, 4]
of which 2 are surviving nonsolos
reads assigned: 542
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = true
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1433 = GCTTGAAGTTACTGAC-1

using 226 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[221]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode GCTTGAAGTTACTGAC-1 contig 1 = GATACTTTCT...
umi AGAAACATCG = 216 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=647]
38-386 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=11)
415-465 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
465-647 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 32 reads
cdr3 = CARDLLWFGETRAFDIW at 383, score = 9 + 8
umis assigned: [2]
of which 0 are surviving nonsolos
reads assigned: 214
start codons at 38, 82, 400, 446
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1437 = GCTTGAAGTTCCCTTG-1

using 142 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[142]
surviving nonsolo ucounts = 1[142]
ids = [0]

====================================================================================

UMI info for barcode GCTTGAAGTTCCCTTG-1 contig 1 = ACCCAAAAAC...
umi TGTTTTGTTC = 135 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=494]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=23)
414-463 ==> 0-49 on |56|IGHJ5|J-REGION| [len=49] (mis=5)
463-494 ==> 0-31 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARGSTSWNDYW at 396, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 134
start codons at 54, 205, 252, 257, 289, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1439 = GCTTGAAGTTGTCTTT-1

using 173 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 167]
surviving nonsolo ucounts = 1[167]
ids = [5]

====================================================================================

UMI info for barcode GCTTGAAGTTGTCTTT-1 contig 1 = AAACCACACC...
umi TTTCATTTCT = 157 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=514]
0-48 ==> 11-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
48-401 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
450-484 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
484-514 ==> 0-30 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 390, score = 7 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 48, 246, 251, 268, 312, 345
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1442 = GCTTGAATCAAGCCTA-1

using 564 reads

====================================================================================

graph has 168 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2, 3^2, 232, 317]
surviving nonsolo ucounts = 2[232, 317]
ids = [11, 5]

====================================================================================

UMI info for barcode GCTTGAATCAAGCCTA-1 contig 1 = ACTTTCTGAG...
umi TTCATTTAGT = 215 reads: +439 validated

UMI info for barcode GCTTGAATCAAGCCTA-1 contig 2 = GATCAGGACT...
umi CTTCGCCTGT = 306 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=482]
0-35 ==> 66-101 on |195|IGHV4-61|5'UTR| [len=101] (mis=0)
35-391 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=4)
423-474 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CARAVRPGNTLLPGTNWFDPW at 380, score = 9 + 7
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 35, 79
confident = false

TIG 2[bases=517]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-517 ==> 0-87 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 304
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1445 = GCTTGAATCACCTTAT-1

using 17 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1449 = GCTTGAATCAGTCCCT-1

using 321 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 313]
surviving nonsolo ucounts = 1[313]
ids = [4]

====================================================================================

UMI info for barcode GCTTGAATCAGTCCCT-1 contig 1 = AGGAGTCAGA...
umi TCACTTGCCA = 298 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=495]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
421-495 ==> 0-74 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQHYNSFFLRYIF at 354, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 27, 33, 89, 102, 238, 334, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1458 = GCTTGAATCCCAAGAT-1

using 558 reads

====================================================================================

graph has 248 edges initially, 30 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 252, 300]
surviving nonsolo ucounts = 2[252, 300]
ids = [1, 0]

====================================================================================

UMI info for barcode GCTTGAATCCCAAGAT-1 contig 1 = AGGAATCAGT...
umi CATTAACCAG = 242 reads: +22 -1XX +365 invalidated

UMI info for barcode GCTTGAATCCCAAGAT-1 contig 2 = AGGAGTCAGA...
umi ATACCTTTCA = 291 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=497]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=18)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-497 ==> 0-82 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 234
start codons at 27, 33, 102, 238, 457
confident = false

TIG 2[bases=497]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=10)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-497 ==> 0-82 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CQQANSFPLTF at 354, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 27, 33, 89, 102, 241, 259, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1459 = GCTTGAATCGGAAATA-1

using 15372 reads

====================================================================================

graph has 4910 edges initially, 32 edges after simplification

total ucounts = 548
nonsolo ucounts = 236[2^77, 3^37, 4^29, 5^19, 6^11, 7^6, 8^2, 9^2, 10, 11, 13, 21, 69, 118, 125, 140, 174, 175, 186, 188, 194, 199, 202, 207, 216, 229, 233, 238, 249, 250, 256, 259, 273^2, 275, 277, 282, 283, 293, 301, 310, 315, 317, 319, 323, 325^2, 327, 334, 343, 354, 361, 364, 375^2, 378, 409, 412, 575, 642, 740]
surviving nonsolo ucounts = 47[118, 125, 140, 174, 175, 186, 188, 194, 199, 202, 207, 216, 229, 233, 238, 249, 250, 256, 259, 273^2, 275, 277, 282, 283, 293, 301, 310, 315, 317, 319, 323, 325^2, 327, 334, 343, 354, 361, 364, 375^2, 378, 409, 412, 575, 740]
ids = [266, 96, 501, 61, 222, 142, 102, 537, 162, 294, ...]

====================================================================================

UMI info for barcode GCTTGAATCGGAAATA-1 contig 1 = GCAGAGCCTG...
umi ACCCGAGGAG = 175 reads: +415 validated
umi ATACCCACCC = 189 reads: +415 validated
umi ATCGTACCCC = 314 reads: +415 validated
umi CAACACAGGG = 188 reads: +415 validated
umi CAACTTCCCG = 279 reads: +415 validated
umi CCTATGCTTC = 176 reads: +415 validated
umi CGCCCTTCCT = 287 reads: +234 -1XX +180 invalidated
umi CTCAAGATCC = 118 reads: +415 validated
umi TCCAACTGGG = 229 reads: +415 validated
umi TGCTCTTTGC = 142 reads: +415 validated
umi TTCTGGTCCT = 815 reads: -382X +1 -5XX +1 -2XX +1 -1XX +1 -10XX +1 -2XX +1 -2XX +1 -3XX +1 invalidated
umi TTTAACAACA = 189 reads: +415 validated

UMI info for barcode GCTTGAATCGGAAATA-1 contig 2 = GGGGAGGAGT...
umi AAACCTGCCG = 275 reads: +385 validated
umi AATTCCCAAT = 363 reads: +385 validated
umi ACAGCCACAC = 261 reads: +385 validated
umi ACGTGCATCG = 321 reads: +385 validated
umi AGTGTACTCC = 122 reads: +385 validated
umi CAAGGACCCG = 311 reads: +385 validated
umi CAATCCCTGG = 328 reads: +385 validated
umi CACCGGATAA = 384 reads: +385 validated
umi CACTATCCTC = 270 reads: +385 validated
umi CAGATCAGGG = 198 reads: +385 validated
umi CAGTGTCCTG = 231 reads: +385 validated
umi CCAATATCCT = 327 reads: +385 validated
umi CCAGCGTATC = 290 reads: +385 validated
umi CCATGTTGGA = 330 reads: +385 validated
umi CCCAGGTGGC = 281 reads: +385 validated
umi CCCCCGAGCG = 379 reads: +385 validated
umi CCCTGGCTCA = 380 reads: +385 validated
umi CGAACTTTCT = 414 reads: +385 validated
umi CTTAGACTGT = 242 reads: +385 validated
umi CTTGGGATGC = 203 reads: +385 validated
umi GACACAGCAG = 250 reads: +385 validated
umi GAGTATAGCC = 256 reads: +385 validated
umi GCCGTCCCCT = 209 reads: +385 validated
umi GTAACAGCTG = 346 reads: +385 validated
umi GTACAAACCA = 355 reads: +385 validated
umi TACCCAAACA = 284 reads: +385 validated
umi TACCCAGCTC = 320 reads: +375 -1XX +9 invalidated
umi TAGAGAGTGG = 254 reads: +385 validated
umi TAGCGTCCTC = 326 reads: +385 validated
umi TCAGAGGCTC = 335 reads: +385 validated
umi TCTGGCCCTC = 366 reads: +385 validated
umi TTGCGTTGAG = 218 reads: +385 validated
umi TTTAACTCGC = 302 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=649]
0-52 ==> 0-52 on |206|IGHV6-1|5'UTR| [len=52] (mis=0)
52-417 ==> 0-365 on |207|IGHV6-1|L-REGION+V-REGION| [len=365] (mis=10)
419-467 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
467-649 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 11 umis using 274 reads
cdr3 = CTRGDTFDYW at 406, score = 10 + 6
umis assigned: [61, 102, 115, 142, 146, 222, 237, 266, 452, 501] and 2 others
of which 11 are surviving nonsolos
reads assigned: 2762
start codons at 52, 96, 293, 299
confident = true

TIG 2[bases=552]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=13)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 32 umis using 1480 reads
cdr3 = CQQFGSLPSF at 358, score = 9 + 7
umis assigned: [4, 44, 53, 73, 96, 147, 149, 153, 159, 162] and 23 others
of which 33 are surviving nonsolos
reads assigned: 9582
start codons at 31, 37, 93, 106, 245, 458
confident = true
now this is a cell
paired!

TTGAACTCTGTGACTCCCGAGGACACGGCTGTCTATTATTGTACAAGAGGAGACACGTTTGACTACTGGGGCCAGGGAATCCTGGTCACCGTCTCCTCAG <==> ACCATCACCAGCCTGCAGCCTGAAGATATTGCAACATATTATTGTCAACAGTTTGGTAGTCTCCCCTCTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1462 = GCTTGAATCGGTTCGG-1

using 62 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^3, 3^3, 4, 5^3, 6, 7, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1467 = GCTTGAATCTCTTATG-1

using 542 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 89, 445]
surviving nonsolo ucounts = 2[89, 445]
ids = [3, 4]

====================================================================================

UMI info for barcode GCTTGAATCTCTTATG-1 contig 1 = GAGCTACAAC...
umi GTTGGGCTCA = 84 reads: -396X +8 -2X +3 invalidated
umi TACATGCTTC = 445 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=575]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-78 ==> 0-48 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
87-402 ==> 48-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=17)
400-439 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
439-575 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CQQYYTAARSF at 378, score = 9 + 7
umis assigned: [3, 4]
of which 2 are surviving nonsolos
reads assigned: 522
start codons at 30, 108, 361, 481
confident = false
see insertion of GTGACTGCA at pos 48 on |296|IGKV4-1|L-REGION+V-REGION|
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1469 = GCTTGAATCTGGCGAC-1

using 289 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 7, 272]
surviving nonsolo ucounts = 1[272]
ids = [8]

====================================================================================

UMI info for barcode GCTTGAATCTGGCGAC-1 contig 1 = AGCTTCAGCT...
umi TCTTGTACAC = 262 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=591]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-591 ==> 0-156 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1470 = GCTTGAATCTGTCTCG-1

using 10224 reads

====================================================================================

graph has 5268 edges initially, 70 edges after simplification

total ucounts = 794
nonsolo ucounts = 379[2^136, 3^65, 4^56, 5^27, 6^25, 7^14, 8^6, 9, 11, 14, 15, 21, 25, 26, 31, 34, 41, 53, 63, 76, 80, 83, 92, 108, 117, 120, 126, 129, 137, 154, 157, 158, 171, 202, 208, 211, 213, 218, 222, 225, 227, 238^2, 239, 241, 250, 251, 252^2, 261, 273, 285, 297, 302, 358, 561, 612]
surviving nonsolo ucounts = 34[41, 53, 63, 76, 83, 108, 117, 120, 157, 158, 171, 202, 208, 211, 213, 218, 222, 225, 227, 238^2, 239, 241, 250, 251, 252^2, 261, 273, 285, 302, 358, 561, 612]
ids = [741, 566, 284, 23, 160, 212, 132, 431, 553, 441, ...]

====================================================================================

UMI info for barcode GCTTGAATCTGTCTCG-1 contig 1 = GCTGCGGGTA...
umi AAGGAACCGC = 256 reads: +388 validated
umi AAGGTCGGGT = 277 reads: +388 validated
umi AATAATCTTT = 219 reads: +388 validated
umi AATCTGGCTT = 239 reads: +388 validated
umi ACGGGTAGGT = 285 reads: +388 validated
umi AGAGTTTGGC = 239 reads: +388 validated
umi AGATGGCAAC = 219 reads: +388 validated
umi CAGTGATCTG = 253 reads: +388 validated
umi CCGCAATCCT = 236 reads: +388 validated
umi CTCCGGTGCT = 265 reads: +388 validated
umi GACGCCATAT = 225 reads: +388 validated
umi GACTCTGGTT = 124 reads: +388 validated
umi GATATCCTCC = 238 reads: +388 validated
umi GGGCGTACTT = 256 reads: +388 validated
umi GTATCGTGGA = 76 reads: -351X +1 -2XX +1 -1XX +1 -2XX +2 -4XX +2 -4XX +1 -9XX +1 -4XX +2 invalidated
umi TAAATACCTT = 154 reads: +388 validated
umi TAGCTTACTT = 212 reads: +388 validated
umi TCAGTGGTAT = 207 reads: +388 validated
umi TTCCACCACA = 40 reads: +388 validated
umi TTTTGACTCT = 622 reads: -346X +1 -2XX +2 -2XX +3 -1XX +31 invalidated

UMI info for barcode GCTTGAATCTGTCTCG-1 contig 2 = CACTCTGCTG...
umi AAGACAATTC = 55 reads: +342 -21 +40 non-validated
umi AGACATTTGG = 256 reads: +403 validated
umi AGTTTACTCG = 109 reads: +403 validated
umi ATCGGCTTTC = 84 reads: +383 -1 +7 -1 +11 non-validated
umi CACCCCCCCC = 97 reads: +403 validated
umi CCCTCTCTTA = 62 reads: +403 validated
umi CCGTCCTCCA = 223 reads: -125 +278 non-validated
umi CTCATTTTCG = 215 reads: +403 validated
umi GACATGCTTT = 202 reads: +403 validated
umi GATCAATCAA = 157 reads: +403 validated
umi TAATCGGTTT = 55 reads: +153 -1XX +240 -1 +8 invalidated
umi TACATTTCGA = 198 reads: +403 validated
umi TCAGATATGC = 367 reads: +403 validated
umi TCCGTTTTTA = 249 reads: +403 validated
umi TTTCCTCTCC = 172 reads: +357 -1 +21 -24 non-validated

GOOD CONTIGS

TIG 1[bases=639]
0-40 ==> 0-40 on |318|IGLV1-36|5'UTR| [len=40] (mis=0)
40-393 ==> 0-353 on |319|IGLV1-36|L-REGION+V-REGION| [len=353] (mis=2)
390-428 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
428-639 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 18 umis using 601 reads
cdr3 = CAAWDDSLNGVVF at 361, score = 8 + 8
umis assigned: [28, 31, 38, 48, 82, 101, 103, 234, 289, 374] and 10 others
of which 19 are surviving nonsolos
reads assigned: 4564
start codons at 40, 191, 248, 251, 344, 369, 374, 386
confident = true

TIG 2[bases=581]
0-107 ==> 129-236 on |170|IGHV3-74|5'UTR| [len=236] (mis=0)
107-460 ==> 0-353 on |171|IGHV3-74|L-REGION+V-REGION| [len=353] (mis=4)
476-510 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
510-581 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 13 umis using 280 reads
cdr3 = CATFGELSGW at 449, score = 9 + 7
umis assigned: [23, 100, 132, 160, 212, 284, 294, 369, 425, 441] and 5 others
of which 15 are surviving nonsolos
reads assigned: 2440
start codons at 107, 263, 324, 410, 475
confident = true
now this is a cell
paired!

ATGAACAGTCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCAACGTTCGGGGAGTTATCAGGATGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1487 = GGAAAGCAGATGTAAC-1

using 1192 reads

====================================================================================

graph has 1320 edges initially, 14 edges after simplification

total ucounts = 391
nonsolo ucounts = 154[2^64, 3^33, 4^19, 5^13, 6^6, 7^7, 8^6, 11, 14, 15^2, 17, 382]
surviving nonsolo ucounts = 1[382]
ids = [313]

====================================================================================

UMI info for barcode GGAAAGCAGATGTAAC-1 contig 1 = GGAGAAGAGC...
umi TCCTTAGGTT = 355 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=491]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=12)
380-418 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
418-491 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYGPSPVF at 360, score = 9 + 7
umis assigned: [313]
of which 1 are surviving nonsolos
reads assigned: 349
start codons at 36, 244, 247, 370, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1488 = GGAAAGCAGCACAGGT-1

using 366 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 359]
surviving nonsolo ucounts = 1[359]
ids = [4]

====================================================================================

UMI info for barcode GGAAAGCAGCACAGGT-1 contig 1 = GAGTCAGACC...
umi TCTCTTATGA = 365 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=549]
0-31 ==> 22-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
31-376 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
385-413 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQYYSYPRTF at 352, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 358
start codons at 25, 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1491 = GGAAAGCAGGAACTGC-1

using 1256 reads

====================================================================================

graph has 1472 edges initially, 10 edges after simplification

total ucounts = 448
nonsolo ucounts = 177[2^72, 3^35, 4^19, 5^15, 6^13, 7^9, 8^6, 9^2, 10^2, 12^2, 14, 320]
surviving nonsolo ucounts = 1[320]
ids = [291]

====================================================================================

UMI info for barcode GGAAAGCAGGAACTGC-1 contig 1 = TGATCAGGAC...
umi TAAAATATAG = 281 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=493]
0-31 ==> 5-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=0)
31-391 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=0)
393-431 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
431-493 ==> 0-62 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CMQALQTPPKTF at 367, score = 9 + 8
umis assigned: [291]
of which 1 are surviving nonsolos
reads assigned: 276
start codons at 31, 64, 100, 188, 350, 370, 473
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1497 = GGAAAGCAGGCTCTTA-1

using 9910 reads

====================================================================================

graph has 7147 edges initially, 105 edges after simplification

total ucounts = 1131
nonsolo ucounts = 852[2^130, 3^94, 4^83, 5^74, 6^77, 7^61, 8^56, 9^40, 10^40, 11^29, 12^26, 13^32, 14^31, 15^16, 16^7, 17^10, 18^7, 19^8, 20^4, 21^2, 22^3, 23, 24, 25^3, 26, 81, 136, 186, 197, 212, 214, 223, 248, 249, 256, 257, 265, 269, 286, 288, 306]
surviving nonsolo ucounts = 16[81, 136, 186, 197, 212, 214, 223, 248, 249, 256, 257, 265, 269, 286, 288, 306]
ids = [909, 815, 340, 41, 820, 179, 519, 56, 788, 131, ...]

====================================================================================

UMI info for barcode GGAAAGCAGGCTCTTA-1 contig 1 = AGGAGTTTCC...
umi CACCGAGTCA = 236 reads: +430 validated
umi CCAGGATTGA = 3 reads: -137 +56 -23 +41 -1 +61 -111 non-validated

UMI info for barcode GGAAAGCAGGCTCTTA-1 contig 2 = GGGGGCTGGG...
umi AAGGTCAGGC = 196 reads: +385 validated
umi AATCGGAATG = 249 reads: +385 validated
umi ACGTGTCCTT = 254 reads: +385 validated
umi AGACAGCTTA = 269 reads: +385 validated
umi AGGCCCGGAT = 216 reads: +385 validated
umi CAGGGTGGTA = 186 reads: +385 validated
umi CGACTGGTGG = 273 reads: +385 validated
umi CGTACGTGTG = 223 reads: +385 validated
umi CGTATGACGA = 310 reads: +385 validated
umi GTCGTGTACT = 250 reads: +385 validated
umi GTTAGCGTCG = 137 reads: +385 validated
umi GTTCTTGGAA = 214 reads: +385 validated
umi TATCCGTTAG = 261 reads: +385 validated
umi TCATCTGTCT = 78 reads: +385 validated
umi TTAATAGGTA = 291 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=501]
0-45 ==> 35-80 on |143|IGHV3-49|5'UTR| [len=80] (mis=0)
45-404 ==> 0-359 on |144|IGHV3-49|L-REGION+V-REGION| [len=359] (mis=5)
401-432 ==> 0-31 on |21|IGHD3-3|D-REGION| [len=31] (mis=5)
429-475 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
475-501 ==> 0-26 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CTSNYDFWSGYYGTDYW at 393, score = 8 + 7
umis assigned: [309, 403]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 45, 98, 196, 201, 268, 325, 354, 427
confident = true

TIG 2[bases=641]
45-393 ==> 0-348 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
430-641 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 15 umis using 552 reads
cdr3 = CSSYTSSSVVF at 369, score = 8 + 8
umis assigned: [41, 56, 131, 161, 179, 340, 488, 519, 521, 788] and 5 others
of which 15 are surviving nonsolos
reads assigned: 3353
start codons at 45, 202, 246, 253, 256
confident = true
now this is a cell
paired!

GACACAGCCGTGTATTACTGTACATCTAATTACGATTTTTGGAGTGGTTATTATGGGACTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCTCTGGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCAGCTCATATACAAGCAGCAGCGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1500 = GGAAAGCAGTAATCCC-1

using 276 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[12, 260]
surviving nonsolo ucounts = 1[260]
ids = [5]

====================================================================================

UMI info for barcode GGAAAGCAGTAATCCC-1 contig 1 = TGGGGGCTTT...
umi TGCGCCAACA = 259 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=576]
19-390 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
407-455 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
455-576 ==> 0-121 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 29 reads
cdr3 = CARYFAFVNYYFDKW at 379, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 19, 40, 84
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1508 = GGAAAGCCAAAGAATC-1

using 429 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^2, 3^2, 4, 409]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1509 = GGAAAGCCAACACCTA-1

using 11379 reads

====================================================================================

graph has 7523 edges initially, 114 edges after simplification

total ucounts = 927
nonsolo ucounts = 424[2^159, 3^91, 4^41, 5^20, 6^19, 7^13, 8^7, 9^8, 10^3, 11^4, 12^3, 13^2, 14^2, 15^4, 16, 17, 18^2, 20^2, 21, 22, 27, 30, 32, 46, 68, 94, 116, 122, 139, 142, 156^2, 160, 184, 201, 212, 228, 233, 240, 243, 253, 256, 259, 262, 263^2, 268^2, 273, 276, 283, 286, 289, 317, 344, 373, 445, 452, 479, 574]
surviving nonsolo ucounts = 37[32, 46, 68, 94, 116, 122, 139, 142, 156, 160, 184, 201, 212, 228, 233, 240, 243, 253, 256, 259, 262, 263^2, 268^2, 273, 276, 283, 286, 289, 317, 344, 373, 445, 452, 479, 574]
ids = [535, 639, 76, 374, 153, 250, 820, 348, 442, 626, ...]

====================================================================================

UMI info for barcode GGAAAGCCAACACCTA-1 contig 1 = TGAGCGCAGA...
umi AAGTACGTAC = 310 reads: +388 validated
umi AATATAAGGG = 145 reads: +21 -1XX +1 -2XX +1 -2XX +1 -5XX +1 -1XX +1 -6XX +337 -8 invalidated
umi AGTAGTGCCA = 259 reads: +388 validated
umi CACGAAGACA = 569 reads: -384 +4 non-validated
umi CACGATGTAT = 121 reads: +388 validated
umi CCGTCGACAC = 261 reads: +388 validated
umi CTTAGGAACT = 339 reads: +388 validated
umi GGCATGTCTT = 220 reads: -7 +1 -2X +1 -5XX +2 -2XX +1 -1XX +1 -2XX +1 -2XX +1 -5XX +1 -1XX +1 -6XX +345 invalidated
umi GTACATACGC = 256 reads: +388 validated
umi TACATATACG = 288 reads: +388 validated
umi TCCTGACCAG = 282 reads: +388 validated
umi TCTTCCACTA = 240 reads: +388 validated
umi TGAAGGGCCA = 95 reads: +21 -1X +1 -2XX +1 -2XX +1 -5XX +1 -1XX +1 -6XX +345 invalidated
umi TTGATTCCGT = 250 reads: +388 validated

UMI info for barcode GGAAAGCCAACACCTA-1 contig 2 = ACTCTGCTGA...
umi AACGGCTCCT = 247 reads: +407 -2 non-validated
umi ACCGATCCAC = 68 reads: +96 -1XX +273 -27 +12 invalidated
umi AGGCTACCTC = 366 reads: +409 validated
umi AGTCCGGGTA = 116 reads: +105 -1 +238 -1 +13 -3 +48 non-validated
umi ATGCCCTCCT = 270 reads: +404 -4 +1 non-validated
umi ATTGGTTCGT = 237 reads: +105 -1 +303 non-validated
umi CAATGGACTC = 278 reads: +399 -10 non-validated
umi CATTATCCCT = 226 reads: +409 validated
umi CCCCGGCTCG = 180 reads: +385 -24 non-validated
umi CCGTCTTCCC = 141 reads: +96 -1XX +312 invalidated
umi CCTTAAGTGC = 95 reads: +94 -1X +10 -1XX +303 invalidated
umi GGGCTCCTTA = 253 reads: +105 -1X +303 invalidated

GOOD CONTIGS

TIG 1[bases=635]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-361 ==> 0-325 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=15)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
424-635 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 12 umis using 417 reads
cdr3 = CAIWDSSLAVGVF at 357, score = 6 + 8
umis assigned: [37, 42, 152, 249, 250, 346, 505, 612, 651, 704] and 4 others
of which 14 are surviving nonsolos
reads assigned: 3592
start codons at 36, 247, 365
confident = true

TIG 2[bases=586]
0-106 ==> 130-236 on |170|IGHV3-74|5'UTR| [len=236] (mis=0)
106-459 ==> 0-353 on |171|IGHV3-74|L-REGION+V-REGION| [len=353] (mis=33)
465-515 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
515-586 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 7 umis using 107 reads
cdr3 = CARGRSDAFDIW at 448, score = 9 + 8
umis assigned: [26, 76, 145, 153, 188, 210, 234, 287, 324, 348] and 2 others
of which 12 are surviving nonsolos
reads assigned: 2408
start codons at 106, 262, 300, 323, 467
confident = true
now this is a cell
paired!

AGTCTGAGAGCCGAGGATACGGCTGTATATTATTGTGCAAGAGGGAGATCTGATGCATTTGATATCTGGGGCCAAGGGACACAGGTCACCGTCTCCTCAG <==> GGACTCCAGACTGGGGACGAGGCCGATTATCACTGCGCAATATGGGATAGCAGTCTGGCTGTTGGGGTGTTCGGCGGCGGGACCAAGTTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1511 = GGAAAGCCAACTGCGC-1

using 5572 reads

====================================================================================

graph has 2664 edges initially, 72 edges after simplification

total ucounts = 429
nonsolo ucounts = 162[2^60, 3^29, 4^19, 5^12, 6^9, 7^4, 8^3, 9^3, 11, 12, 15, 16, 18, 142, 155, 168, 185, 201, 234, 240, 244, 257, 258^2, 280, 283, 289, 310, 313, 334, 605]
surviving nonsolo ucounts = 18[142, 155, 168, 185, 201, 234, 240, 244, 257, 258^2, 280, 283, 289, 310, 313, 334, 605]
ids = [241, 166, 243, 412, 128, 263, 409, 204, 187, 62, ...]

====================================================================================

UMI info for barcode GGAAAGCCAACTGCGC-1 contig 1 = TGAGCGCAGA...
umi AACATTCATC = 285 reads: +388 validated
umi AAGAGCTCGG = 18 reads: -345 +1 -1X +1 -3XX +1 -2XX +2 -1XX +5 -2XX +1 -1XX +9 -1XX +1 -1XX +10 invalidated
umi ACCTTGCCTC = 615 reads: -344X +1 -9XX +2 -1XX +5 -2XX +1 -1XX +9 -1XX +1 -1XX +10 invalidated
umi ACTCTGCGTC = 252 reads: +388 validated
umi CATAGATCCA = 201 reads: +388 validated
umi CGCCGAACCT = 284 reads: +388 validated
umi CTAAGGCACT = 120 reads: +38 -5X +345 invalidated
umi GAGGCTCTGT = 241 reads: +388 validated
umi GGGGCACCCT = 143 reads: +388 validated
umi GGTATTATGG = 164 reads: +388 validated
umi GTGACCCCGG = 229 reads: +388 validated
umi GTTGCGACCC = 309 reads: +388 validated
umi TATCCACCAG = 257 reads: +388 validated
umi TTGCAAAGCA = 234 reads: +388 validated
umi TTGTACCCTC = 178 reads: +388 validated

UMI info for barcode GGAAAGCCAACTGCGC-1 contig 2 = ATACTTTCTG...
umi CCCCCTCTTC = 283 reads: +415 validated
umi TTGATCGTAC = 266 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=635]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=6)
386-424 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
424-635 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 13 umis using 411 reads
cdr3 = CGTWDSSLSAYVF at 357, score = 7 + 8
umis assigned: [13, 18, 51, 62, 128, 151, 166, 204, 241, 243] and 5 others
of which 15 are surviving nonsolos
reads assigned: 3465
start codons at 36, 190, 241, 365, 388, 556
confident = true

TIG 2[bases=554]
0-37 ==> 108-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
37-387 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=11)
390-406 ==> 0-16 on |25|IGHD4-17|D-REGION| [len=16] (mis=3)
404-452 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
452-554 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 2 umis using 55 reads
cdr3 = CARGVDYGDLTLDYW at 376, score = 9 + 7
umis assigned: [138, 408]
of which 2 are surviving nonsolos
reads assigned: 541
start codons at 37, 81, 470, 531
confident = true
now this is a cell
paired!

GCTGCGGACACGGCCGTGTATTACTGTGCGAGAGGGGTTGACTACGGTGACCTAACCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGACTCCAGACTGGGGACGAGGCCGATTATTACTGCGGAACATGGGATAGCAGCCTGAGTGCGTATGTCTTCGGAAGTGGGACCAAGGTCACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1520 = GGAAAGCCACAAGTAA-1

using 380 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 371]
surviving nonsolo ucounts = 1[371]
ids = [4]

====================================================================================

UMI info for barcode GGAAAGCCACAAGTAA-1 contig 1 = GGTGCTTTCT...
umi TCGGCGATTT = 375 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=527]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=22)
410-456 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=8)
456-527 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CARGSDPSATVILDYW at 377, score = 9 + 6
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 370
start codons at 17, 38, 49, 82, 112, 168, 221
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1525 = GGAAAGCCACCTTGTC-1

using 188 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4^2, 179]
surviving nonsolo ucounts = 1[179]
ids = [3]

====================================================================================

UMI info for barcode GGAAAGCCACCTTGTC-1 contig 1 = GCTCTGCTTC...
umi TTGTTCGGAC = 173 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=546]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-546 ==> 0-101 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 171
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1527 = GGAAAGCCACTCGACG-1

using 6185 reads

====================================================================================

graph has 2818 edges initially, 52 edges after simplification

total ucounts = 388
nonsolo ucounts = 162[2^56, 3^31, 4^15, 5^9, 6^5, 7^7, 8^3, 9^8, 10, 11^2, 13, 15^2, 16, 17, 18, 97, 116, 123, 160, 169, 173, 174, 215, 226, 250, 253, 260, 276, 284, 377, 386, 408, 413, 988]
surviving nonsolo ucounts = 18[116, 123, 160, 169, 173, 174, 215, 226, 250, 253, 260, 276, 284, 377, 386, 408, 413, 988]
ids = [331, 183, 308, 320, 159, 145, 38, 130, 257, 9, ...]

====================================================================================

UMI info for barcode GGAAAGCCACTCGACG-1 contig 1 = GATGGAGTTT...
umi CCGTTGTGTT = 218 reads: +439 validated
umi TGAATGCGTT = 117 reads: +439 validated
umi TGGTGTATAT = 258 reads: +439 validated

UMI info for barcode GGAAAGCCACTCGACG-1 contig 2 = CTGGGCCTCA...
umi AACAGTCTTG = 248 reads: +385 validated
umi AAGAGAACAT = 283 reads: +385 validated
umi ACCTCCGATT = 216 reads: +385 validated
umi CCCTGCTCTG = 410 reads: -103X +1 -1XX +1 -4XX +1 -3XX +1 -3XX +267 invalidated
umi CGGCACGCAG = 175 reads: +385 validated
umi CTATAGCAAC = 173 reads: +385 validated
umi GAGCTACCAG = 376 reads: +385 validated
umi GGCTTGATAC = 420 reads: +385 validated
umi GTGACCCCCC = 253 reads: +385 validated
umi TATTTCTGAA = 159 reads: +385 validated
umi TCCGCGACTT = 171 reads: +385 validated
umi TGCATTAAGG = 257 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=558]
1-352 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=27)
360-391 ==> 0-31 on |14|IGHD2-2|D-REGION| [len=31] (mis=4)
389-440 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
440-558 ==> 0-118 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 3 umis using 76 reads
cdr3 = CAKSPAGYCSRTSCYDNWFDPW at 343, score = 8 + 7
umis assigned: [130, 331, 347]
of which 3 are surviving nonsolos
reads assigned: 583
start codons at 1, 157, 218, 236, 304, 494
confident = true

TIG 2[bases=633]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-383 ==> 0-346 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=21)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
422-633 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 12 umis using 558 reads
cdr3 = CQVWDSNTALEVVF at 352, score = 6 + 8
umis assigned: [9, 18, 38, 125, 145, 159, 196, 237, 257, 308] and 2 others
of which 12 are surviving nonsolos
reads assigned: 3093
start codons at 37, 42, 98, 335
confident = true

REJECT CONTIGS

TIG 1[bases=305]
5-245 ==> 11262-11502 on segment before IGLV3-6 exon 1 [len=11502] (mis=2)
umis assigned: [183, 310]
of which 2 are surviving nonsolos
reads assigned: 1054
start codons at 40, 247
confident = false
did not find CDR3
now this is a cell
paired!

TACTGTGCGAAAAGTCCCGCCGGATATTGTAGTAGGACCAGCTGCTACGACAACTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ACCCAGGCTGTGGATGAGGCTGACTATTATTGTCAGGTGTGGGACAGCAACACTGCTTTGGAAGTGGTTTTCGGCGGAGGGACCAAACTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1528 = GGAAAGCCACTGTCGG-1

using 39 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 1[37]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1529 = GGAAAGCCAGACACTT-1

using 243 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[243]
surviving nonsolo ucounts = 1[243]
ids = [0]

====================================================================================

UMI info for barcode GGAAAGCCAGACACTT-1 contig 1 = GTCACAAGAG...
umi TAGTGAAGCA = 233 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=523]
0-35 ==> 127-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
35-375 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=24)
392-420 ==> 10-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
420-523 ==> 0-103 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 36 reads
cdr3 = CFSYGTSGRTF at 359, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 231
start codons at 35, 243, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1530 = GGAAAGCCAGAGCCAA-1

using 123 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 117]
surviving nonsolo ucounts = 1[117]
ids = [2]

====================================================================================

UMI info for barcode GGAAAGCCAGAGCCAA-1 contig 1 = GCTGGGGTCT...
umi GAGAACACTG = 115 reads: +388 validated
umi TATTGTGAGG = 2 reads: -169 +7 -1X +24 -1X +14 -2X +3 -1XX +14 -2X +2 -1X +35 -112 invalidated

GOOD CONTIGS

TIG 1[bases=464]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=1)
41-356 ==> 0-315 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=12)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
429-464 ==> 0-35 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CTSYAGGSNVVF at 365, score = 8 + 8
umis assigned: [2, 4]
of which 1 are surviving nonsolos
reads assigned: 115
start codons at 41, 249, 348, 375, 390
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1532 = GGAAAGCCAGCATGAG-1

using 468 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 162, 298]
surviving nonsolo ucounts = 2[162, 298]
ids = [3, 4]

====================================================================================

UMI info for barcode GGAAAGCCAGCATGAG-1 contig 1 = AGTGACTCCT...
umi GCATTACTCT = 156 reads: +433 validated

UMI info for barcode GGAAAGCCAGCATGAG-1 contig 2 = GGGAGGAACT...
umi GGAACGTATG = 256 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=528]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=19)
403-453 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
453-528 ==> 0-75 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 365, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 20, 64, 243, 246, 249, 335, 405
confident = false

TIG 2[bases=456]
0-35 ==> 12-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
35-380 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=2)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
414-456 ==> 0-42 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQRSNWRTF at 356, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 253
start codons at 35, 240, 243
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1534 = GGAAAGCCAGCTATTG-1

using 1005 reads

====================================================================================

graph has 378 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[4^2, 329, 666]
surviving nonsolo ucounts = 2[329, 666]
ids = [4, 0]

====================================================================================

UMI info for barcode GGAAAGCCAGCTATTG-1 contig 1 = GATCAGGACT...
umi TCCAGTGCGC = 310 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=443]
0-30 ==> 0-30 on |266|IGKV2-30|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=4)
385-424 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
424-443 ==> 0-19 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CMQGTHGYTF at 366, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 306
start codons at 30, 63, 91, 99, 187, 349, 369
confident = false

REJECT CONTIGS

TIG 1[bases=307]
10-35 ==> 4932-4957 on rc of segment before IGLL1 exon 1 [len=6445] (mis=0)
58-96 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
94-142 ==> 6397-6445 on rc of segment before IGLL1 exon 1 [len=6445] (mis=4)
96-307 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 661
start codons at 60, 228
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1538 = GGAAAGCCAGTTAACC-1

using 330 reads

====================================================================================

graph has 166 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^3, 3^3, 5, 305]
surviving nonsolo ucounts = 1[305]
ids = [0]

====================================================================================

UMI info for barcode GGAAAGCCAGTTAACC-1 contig 1 = AGGAATCAGT...
umi AACTGTAGGA = 305 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 300
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1539 = GGAAAGCCATCCGGGT-1

using 371 reads

====================================================================================

graph has 174 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[171, 194]
surviving nonsolo ucounts = 2[171, 194]
ids = [4, 1]

====================================================================================

UMI info for barcode GGAAAGCCATCCGGGT-1 contig 1 = AGCTCTGAGA...
umi CACCGATCGT = 197 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=518]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=19)
459-485 ==> 20-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
485-518 ==> 0-33 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CAKNSGWFGYW at 421, score = 8 + 6
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 191
start codons at 79, 235, 382, 438
confident = false

REJECT CONTIGS

TIG 1[bases=455]
0-26 ==> 0-26 on |303|IGKV6D-41|5'UTR| [len=26] (mis=0)
26-371 ==> 0-345 on |304|IGKV6D-41|L-REGION+V-REGION| [len=345] (mis=8)
384-455 ==> 0-71 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 26, 95, 234, 330, 340, 426
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1542 = GGAAAGCCATGCTAGT-1

using 313 reads

====================================================================================

graph has 118 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 15, 295]
surviving nonsolo ucounts = 1[295]
ids = [2]

====================================================================================

UMI info for barcode GGAAAGCCATGCTAGT-1 contig 1 = GGGGAGGAAC...
umi AACACCTCAA = 3 reads: -126X +24 -1X +27 -1X +2 -3 +6 -1X +38 -1X +10 -145 invalidated
umi GGATTCGAAT = 266 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=504]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=6)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
421-504 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYDSWPGYIF at 357, score = 9 + 8
umis assigned: [0, 2]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 36, 105, 241, 367, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1557 = GGAAAGCGTCTCCACT-1

using 98 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[97]
surviving nonsolo ucounts = 1[97]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=377]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-34 ==> 5703-5737 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=1)
33-377 ==> 0-344 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 84
start codons at 33, 241, 367
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1559 = GGAAAGCGTCTCCCTA-1

using 3002 reads

====================================================================================

graph has 1296 edges initially, 18 edges after simplification

total ucounts = 132
nonsolo ucounts = 77[2^26, 3^2, 4, 5^3, 17, 19, 26, 32, 37^2, 41, 42, 43, 45, 46^2, 49, 50, 51^2, 52^4, 53, 55, 56, 58^4, 59, 60, 62, 63^2, 64, 65, 68, 74^2, 78, 80^2, 84, 92, 155, 184, 229]
surviving nonsolo ucounts = 46[2, 17, 19, 26, 32, 37^2, 41, 42, 43, 45, 46^2, 49, 50, 51^2, 52^4, 53, 55, 56, 58^4, 59, 60, 62, 63^2, 64, 65, 68, 74^2, 78, 80^2, 84, 92, 155, 184, 229]
ids = [85, 24, 23, 47, 66, 104, 116, 72, 67, 121, ...]

====================================================================================

UMI info for barcode GGAAAGCGTCTCCCTA-1 contig 1 = CTCAGAGAGG...
umi AATAATGCCC = 99 reads: -400 +1 -1X +2 -2XX +2 -2XX +1 -10XX +1 -5XX +1 -1XX +1 invalidated
umi ACTCATGTCC = 54 reads: +312 -2X +5 -1 +100 -10 invalidated
umi ATTACAGGTT = 15 reads: -405 +2 -1X +3 -2X +8 -1X +8 invalidated
umi CCCCACGTAC = 240 reads: +46 -4XX +2 -1XX +1 -13XX +1 -2XX +1 -334 +1 -4XX +3 -1XX +3 -1XX +1 -2XX +1 -1XX +1 -4XX +2 invalidated
umi CGCTGAGGGA = 58 reads: +267 -27 +8 -1 +9 -1 +2 -1 +68 -5X +1 -1 +16 -1 +22 invalidated
umi CTGTCGACAT = 47 reads: +11 -1 +2 -1 +1 -2 +295 -12 +1 -2 +4 -2X +20 -1 +57 -18 invalidated
umi CTGTGATCAT = 188 reads: +430 validated
umi CTTGCTTTCC = 33 reads: +202 -5 +113 -13 +66 -1 +6 -1 +6 -1 +16 non-validated
umi GCACCTATCA = 53 reads: +411 -9 +10 non-validated
umi TCCACCTTGC = 66 reads: +425 -1 +1 -1 +1 -1 non-validated
umi TTTCCCACCG = 53 reads: +348 -6 +76 non-validated

UMI info for barcode GGAAAGCGTCTCCCTA-1 contig 2 = AGGAGTCAGT...
umi AAACTCCGCA = 79 reads: +382 validated
umi AATACAGGAA = 51 reads: +382 validated
umi ATGATTTCCC = 84 reads: +382 validated
umi ATGCAACCGT = 19 reads: -32 +175 -1 +3 -1 +42 -1 +21 -1 +82 -1 +3 -1 +18 non-validated
umi ATTCCAAGAC = 59 reads: +382 validated
umi CCCTTTCATG = 48 reads: +322 -1 +59 non-validated
umi CCGAATTGGT = 65 reads: +382 validated
umi CCTAACAGTC = 62 reads: +382 validated
umi CCTACCCTCC = 80 reads: +130 -1XX +251 invalidated
umi CGAAAACATA = 57 reads: +382 validated
umi CGATTAGGTC = 25 reads: +6 -1 +247 -11 +79 -1 +37 non-validated
umi CTACAATATA = 80 reads: +382 validated
umi CTCCTACCTT = 57 reads: +362 -1X +19 invalidated
umi CTTATCCCCA = 50 reads: -7 +1 -1 +3 -1 +369 non-validated
umi CTTGTGTCCG = 42 reads: -5 +377 non-validated
umi CTTTTCTCCA = 78 reads: +185 -1XX +148 -1XX +47 invalidated
umi GAACAATGGG = 63 reads: +362 -1 +3 -1 +15 non-validated
umi GACTCATATC = 42 reads: -9 +373 non-validated
umi GACTGTAGTA = 74 reads: +382 validated
umi GATAAACCCT = 54 reads: +382 validated
umi GATTTCCCTC = 50 reads: +382 validated
umi GGATCGCGAT = 47 reads: +382 validated
umi GTCGACGGCT = 90 reads: +382 validated
umi GTGTATTGCG = 69 reads: +382 validated
umi TAGCAGCCTT = 37 reads: -5 +338 -39 non-validated
umi TCCTGGCTCG = 47 reads: +350 -1 +6 -1 +4 -1 +19 non-validated
umi TCGATACACC = 62 reads: -1 +1 -1 +379 non-validated
umi TCTAATGTGT = 37 reads: +100 -15 +267 non-validated
umi TCTGTCTGGC = 63 reads: +382 validated
umi TCTTTTGGCC = 59 reads: +347 -1 +1 -2 +1 -2X +1 -3X +1 -5X +2 -1 +4 -3 +6 -1 +1 invalidated
umi TGCTTTCGCC = 42 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=665]
0-75 ==> 4-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
75-428 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=39)
459-505 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
505-665 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 2 umis using 21 reads
cdr3 = CARESYVGLYSSTSYPDYW at 417, score = 8 + 7
umis assigned: [4, 13, 24, 34, 48, 61, 62, 66, 77, 110] and 1 others
of which 11 are surviving nonsolos
reads assigned: 874
start codons at 75, 224, 231, 310, 352, 378, 433, 559
confident = true

TIG 2[bases=551]
33-281 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 24 umis using 249 reads
cdr3 = CLHYANRRRTF at 354, score = 9 + 8
umis assigned: [0, 5, 22, 23, 25, 37, 38, 39, 40, 46] and 21 others
of which 31 are surviving nonsolos
reads assigned: 1740
start codons at 33, 89, 102, 241, 364, 457
confident = true
now this is a cell
paired!

GCTGTGTATTACTGTGCGAGAGAATCTTATGTCGGGCTCTATTCTTCAACCAGTTATCCCGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GTCTCCAGCCTGCAACCTGAAGATTTTGCCACCTATTATTGTCTACACTATGCTAATCGGCGTCGCACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1562 = GGAAAGCGTGCCTGCA-1

using 12 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1567 = GGAAAGCGTTGTGGAG-1

using 170 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[2, 168]
surviving nonsolo ucounts = 1[168]
ids = [0]

====================================================================================

UMI info for barcode GGAAAGCGTTGTGGAG-1 contig 1 = AGCTGTGGGC...
umi AGGATTTTGC = 163 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=519]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=21)
390-428 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
428-519 ==> 0-91 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CNSRDSSGNHVNVIF at 355, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 162
start codons at 40, 159, 239, 338, 383, 389
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1568 = GGAAAGCGTTTAGGAA-1

using 34 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[34]
surviving nonsolo ucounts = 1[34]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1582 = GGAAAGCTCCGCGGTA-1

using 142 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 134]
surviving nonsolo ucounts = 1[134]
ids = [2]

====================================================================================

UMI info for barcode GGAAAGCTCCGCGGTA-1 contig 1 = AGAGCTCTGG...
umi GCAAACTCAA = 137 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=565]
0-44 ==> 53-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
44-389 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=9)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQQYSNWPPWTF at 365, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 132
start codons at 44, 113, 140, 249, 348, 471
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1586 = GGAAAGCTCGCGTTTC-1

using 312 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[72, 234]
surviving nonsolo ucounts = 2[72, 234]
ids = [1, 5]

====================================================================================

UMI info for barcode GGAAAGCTCGCGTTTC-1 contig 1 = AGTCTGGGCC...
umi GAGGTTATAG = 221 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=536]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-383 ==> 0-343 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=0)
390-428 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
428-536 ==> 0-108 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQVWDSSSDLHNYVF at 355, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 40, 101, 239, 242, 338, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1587 = GGAAAGCTCGGCATCG-1

using 437 reads

====================================================================================

graph has 244 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 14[2, 3^2, 4^2, 8, 9, 11^4, 12, 16, 328]
surviving nonsolo ucounts = 1[328]
ids = [9]

====================================================================================

UMI info for barcode GGAAAGCTCGGCATCG-1 contig 1 = CTGGGCCTAA...
umi CTGCCCGGCT = 327 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=630]
0-37 ==> 214-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
37-370 ==> 0-333 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=29)
381-419 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
419-630 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQVWDSDGHYVVF at 352, score = 8 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 321
start codons at 37, 98, 167, 335, 371, 380
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1591 = GGAAAGCTCGTAGGAG-1

using 347 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[347]
surviving nonsolo ucounts = 1[347]
ids = [0]

====================================================================================

UMI info for barcode GGAAAGCTCGTAGGAG-1 contig 1 = GGGGAGGAAC...
umi GCAGTAAGCA = 322 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=499]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=17)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
418-499 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CLQYSDWPRTF at 357, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1599 = GGAAAGCTCTGTCCGT-1

using 223 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[220]
surviving nonsolo ucounts = 1[220]
ids = [2]

====================================================================================

UMI info for barcode GGAAAGCTCTGTCCGT-1 contig 1 = AGCTTCAGCT...
umi GGTCCTCTCT = 215 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=583]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-583 ==> 0-148 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1603 = GGAAAGCTCTTTAGTC-1

using 288 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[10, 274]
surviving nonsolo ucounts = 1[274]
ids = [5]

====================================================================================

UMI info for barcode GGAAAGCTCTTTAGTC-1 contig 1 = GGGGAGGAGT...
umi TTGTGCCTGA = 273 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=558]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=1)
383-422 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
422-558 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYDNLPPGTF at 358, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 31, 37, 93, 106, 245, 368, 464
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1604 = GGAACTTAGAAACCGC-1

using 3827 reads

====================================================================================

graph has 1872 edges initially, 24 edges after simplification

total ucounts = 291
nonsolo ucounts = 91[2^35, 3^16, 4^9, 5^7, 6^2, 7^5, 8, 9^2, 10^3, 13^2, 17, 21, 269, 270, 291, 321, 597, 598, 925]
surviving nonsolo ucounts = 7[269, 270, 291, 321, 597, 598, 925]
ids = [207, 206, 265, 141, 12, 126, 156]

====================================================================================

UMI info for barcode GGAACTTAGAAACCGC-1 contig 1 = AGGAGTCAGT...
umi AACTTATTTG = 591 reads: +388 validated
umi CGGATATCCC = 592 reads: +388 validated
umi CTCCTAGCCT = 318 reads: +388 validated
umi GATATTTTGA = 928 reads: -213X +175 invalidated
umi TACAGGGTAT = 63 reads: -240 +3 -3XX +17 -1XX +5 -1XX +5 -1XX +20 -1XX +2 -2XX +1 -1XX +8 -2XX +5 -2XX +3 -1XX +8 -22X +2 -1XX +14 -5XX +4 -1XX +2 -1XX +4 invalidated
umi TACATCCTTG = 41 reads: -240 +3 -3XX +17 -1XX +5 -1XX +5 -1XX +20 -1XX +2 -2XX +1 -1XX +8 -2XX +5 -2XX +3 -34XX +2 -1XX +5 -1XX +5 -4XX +8 -1XX +4 invalidated
umi TTCAAAATCT = 292 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 20-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=16)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 471 reads
cdr3 = CQQSFRRPITF at 354, score = 9 + 8
umis assigned: [12, 126, 141, 156, 206, 207, 265]
of which 7 are surviving nonsolos
reads assigned: 2787
start codons at 27, 33, 89, 102, 238, 457
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1608 = GGAACTTAGACAAGCC-1

using 304 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[12, 289]
surviving nonsolo ucounts = 1[289]
ids = [1]

====================================================================================

UMI info for barcode GGAACTTAGACAAGCC-1 contig 1 = AGAGCTCTGG...
umi CATCGTATAG = 288 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=507]
0-22 ==> 38-60 on |373|IGLV4-69|5'UTR| [len=60] (mis=0)
22-381 ==> 0-359 on |374|IGLV4-69|L-REGION+V-REGION| [len=359] (mis=1)
378-416 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
416-507 ==> 0-91 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CQTWGTGIRVF at 355, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 278
start codons at 22, 183, 223, 239, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1609 = GGAACTTAGACCGGAT-1

using 76 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 7, 63]
surviving nonsolo ucounts = 1[63]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1634 = GGAACTTAGGCTAGCA-1

using 29 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 1[27]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1636 = GGAACTTAGGGCTCTC-1

using 354 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 345]
surviving nonsolo ucounts = 1[345]
ids = [5]

====================================================================================

UMI info for barcode GGAACTTAGGGCTCTC-1 contig 1 = GATCAGGACT...
umi TGCAAATCTT = 335 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=488]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=2)
389-427 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
427-488 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CMQALQTRETF at 366, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 328
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1639 = GGAACTTAGTCAAGCG-1

using 5954 reads

====================================================================================

graph has 4339 edges initially, 74 edges after simplification

total ucounts = 830
nonsolo ucounts = 587[2^113, 3^65, 4^72, 5^59, 6^45, 7^33, 8^36, 9^35, 10^29, 11^14, 12^20, 13^13, 14^12, 15^7, 16^13, 17^4, 18^3, 19^4, 22^2, 24, 120, 203, 232, 252, 257, 259, 680]
surviving nonsolo ucounts = 7[120, 203, 232, 252, 257, 259, 680]
ids = [673, 279, 589, 12, 225, 453, 580]

====================================================================================

UMI info for barcode GGAACTTAGTCAAGCG-1 contig 1 = GGGAGAGGAG...
umi GTCTCCACTT = 225 reads: +409 validated

UMI info for barcode GGAACTTAGTCAAGCG-1 contig 2 = GCTGGGGTCT...
umi AAATATACCG = 253 reads: +388 validated
umi CAATAATTCC = 258 reads: +388 validated
umi CCAATGGTCA = 205 reads: +388 validated
umi GAAGTACGCA = 262 reads: +388 validated
umi GTAGACAACC = 694 reads: -346X +1 -3XX +2 -3XX +1 -1XX +5 -4XX +11 -1XX +10 invalidated
umi TCATGTCCCC = 122 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=514]
0-73 ==> 7-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
73-424 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=25)
434-482 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
482-514 ==> 0-32 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CASAMAFYFEDW at 415, score = 7 + 7
umis assigned: [589]
of which 1 are surviving nonsolos
reads assigned: 222
start codons at 73, 224, 229, 287, 290, 299, 308, 376, 427
confident = true

TIG 2[bases=640]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-395 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=19)
391-429 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
429-640 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=3)
junction support: 5 umis using 162 reads
cdr3 = CTSFAGSNNFIF at 365, score = 8 + 9
umis assigned: [12, 225, 279, 453, 580, 673]
of which 6 are surviving nonsolos
reads assigned: 1736
start codons at 41, 198, 249, 348
confident = true
now this is a cell
paired!

AACCTGAGGGGCGACGACACGGCTGTTTATTACTGTGCGAGTGCCATGGCCTTCTACTTTGAAGACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> TCTGGGCTCCAGGCTGAGGATGAGGCTGACTATTACTGCACCTCATTTGCAGGCAGCAACAATTTCATCTTCGGAACTGGGACCAAGCTCACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1643 = GGAACTTAGTGGTAAT-1

using 579 reads

====================================================================================

graph has 256 edges initially, 8 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 4, 5, 105, 152, 306]
surviving nonsolo ucounts = 3[105, 152, 306]
ids = [3, 0, 7]

====================================================================================

UMI info for barcode GGAACTTAGTGGTAAT-1 contig 1 = CTTTCTGAGA...
umi CAGAGGGCTG = 103 reads: +427 validated

UMI info for barcode GGAACTTAGTGGTAAT-1 contig 2 = AAAAACCACA...
umi GAGAGGTCGT = 304 reads: +436 validated

UMI info for barcode GGAACTTAGTGGTAAT-1 contig 3 = ATCAGTCCCA...
umi AATATCCATT = 141 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=528]
0-34 ==> 111-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
34-384 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=27)
387-403 ==> 0-16 on |25|IGHD4-17|D-REGION| [len=16] (mis=2)
398-461 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=5)
461-528 ==> 0-67 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CARGPHYGDYYFNYYMDVW at 373, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 101
start codons at 34, 78, 158, 239, 418, 515
confident = false

TIG 2[bases=553]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-553 ==> 0-67 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 299
start codons at 50, 248, 253, 270, 314, 347, 540
confident = false

TIG 3[bases=483]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-483 ==> 0-72 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 137
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1650 = GGAACTTCAACGATGG-1

using 19346 reads

====================================================================================

graph has 6393 edges initially, 34 edges after simplification

total ucounts = 833
nonsolo ucounts = 409[2^142, 3^80, 4^51, 5^26, 6^19, 7^7, 8^4, 9^4, 10^4, 11, 14^4, 19, 21, 44, 51, 58, 117, 121, 126, 135, 141, 156, 167, 169, 211^2, 226^2, 233, 234, 244^2, 247, 248, 250, 256, 257, 260, 261, 263^3, 264, 268^2, 269, 279^2, 282, 286, 291, 293, 297, 298, 302, 313, 315^2, 316, 321, 322, 323^2, 325, 327, 330, 332, 333, 336, 338, 339, 343, 355, 383, 386, 424, 487, 741]
surviving nonsolo ucounts = 61[117, 121, 126, 135, 141, 156, 167, 169, 211^2, 226^2, 234, 244^2, 247, 248, 250, 256, 257, 260, 261, 263^3, 264, 268^2, 269, 279^2, 282, 286, 291, 293, 297, 298, 302, 313, 315^2, 316, 321, 322, 323^2, 325, 327, 330, 332, 333, 336, 338, 339, 343, 355, 383, 386, 424, 487, 741]
ids = [538, 248, 3, 514, 808, 789, 36, 6, 458, 469, ...]

====================================================================================

UMI info for barcode GGAACTTCAACGATGG-1 contig 1 = GGGAGAGCCC...
umi AAAGGACCGC = 125 reads: +382 validated
umi AAATGTCCTG = 167 reads: +382 validated
umi AACCGCGTCC = 270 reads: +382 validated
umi AACCTGTCTG = 324 reads: +382 validated
umi AACTAGTACC = 242 reads: +382 validated
umi AAGGATTACG = 264 reads: +382 validated
umi ACACTGCCAT = 493 reads: +382 validated
umi ACGCTCGTAC = 286 reads: +382 validated
umi AGAGTCCCGT = 746 reads: +382 validated
umi AGATTCACCA = 320 reads: +382 validated
umi AGCACATCCT = 419 reads: +382 validated
umi AGTTAGGGCC = 291 reads: +382 validated
umi AGTTCGGTTT = 332 reads: +382 validated
umi ATCCTGGGAT = 322 reads: +382 validated
umi ATGTAAAGGA = 331 reads: +382 validated
umi ATTGAATTCT = 317 reads: +382 validated
umi CAACTGTGGT = 331 reads: +382 validated
umi CATTGAAGTT = 252 reads: +49 -5XX +1 -2XX +1 -6XX +1 -4XX +1 -2XX +1 -13X +1 -3XX +2 -4XX +1 -1XX +1 -3XX +280 invalidated
umi CCCTGTCCTT = 264 reads: +382 validated
umi CCCTTCGCTC = 324 reads: +382 validated
umi CCGCTGTACT = 264 reads: +382 validated
umi CCTTGACTAC = 278 reads: +382 validated
umi CGAAATCACG = 332 reads: +382 validated
umi CGGGTCGTAA = 347 reads: +382 validated
umi CTAGTAATCC = 341 reads: +382 validated
umi CTCACATTCT = 337 reads: +382 validated
umi CTCGTTCCGG = 259 reads: +382 validated
umi CTGAACCTTC = 335 reads: +382 validated
umi GAAAGACATC = 282 reads: +382 validated
umi GATAAACTGT = 34 reads: +49 -1XX +1 -4X +5 -1XX +3 -1XX +40 -1XX +1 -2XX +3 -270 invalidated
umi GCTATGGCGT = 206 reads: +382 validated
umi GGATACGGTC = 284 reads: +382 validated
umi GTCGTGTCTC = 261 reads: +382 validated
umi GTCTGTATTC = 119 reads: +382 validated
umi TACGTTACAT = 299 reads: +382 validated
umi TAGAGTTATC = 349 reads: +382 validated
umi TCACCGTGCA = 317 reads: +382 validated
umi TCACTTGCAG = 232 reads: +382 validated
umi TCAGTTTCCT = 301 reads: +382 validated
umi TCCCATCTGC = 249 reads: +382 validated
umi TCCCGGGCCG = 324 reads: +382 validated
umi TCGACGTCCC = 391 reads: +382 validated
umi TGATGCGGCT = 265 reads: +382 validated
umi TGTATTATAC = 263 reads: +382 validated
umi TGTGGTAAAA = 319 reads: +382 validated
umi TTACCGCGCC = 265 reads: +382 validated
umi TTATTCCGGT = 386 reads: +382 validated
umi TTGAAAGACA = 156 reads: +382 validated
umi TTGTTATTTA = 302 reads: +382 validated
umi TTTAGTAAGC = 140 reads: +382 validated
umi TTTGTACGAA = 243 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=565]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=1)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 50 umis using 2313 reads
cdr3 = CQQRSNWPWTF at 368, score = 9 + 8
umis assigned: [3, 6, 14, 16, 21, 35, 58, 82, 111, 115] and 41 others
of which 50 are surviving nonsolos
reads assigned: 14659
start codons at 47, 252, 255, 471
confident = true

REJECT CONTIGS

TIG 1[bases=562]
1-84 ==> 3251-3334 on rc of segment before IGKV2-24 exon 2 [len=3774] (mis=5)
1-84 ==> 3248-3331 on segment before IGKV2D-23 exon 1 [len=3772] (mis=5)
34-382 ==> 0-348 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=34)
389-426 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
426-562 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [27, 36, 85, 102, 164, 232, 248, 514, 820, 824]
of which 10 are surviving nonsolos
reads assigned: 2225
start codons at 67, 103, 191, 353, 373, 468
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1654 = GGAACTTCAAGCCATT-1

using 189 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 181]
surviving nonsolo ucounts = 1[181]
ids = [0]

====================================================================================

UMI info for barcode GGAACTTCAAGCCATT-1 contig 1 = GAGCTACAAC...
umi ACACAACGCC = 181 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=507]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=23)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
430-507 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQQYFSIPLTF at 369, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 178
start codons at 30, 79, 99, 352, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1662 = GGAACTTCAATCACAC-1

using 1883 reads

====================================================================================

graph has 600 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 3, 8, 311, 593, 964]
surviving nonsolo ucounts = 3[311, 593, 964]
ids = [3, 0, 7]

====================================================================================

UMI info for barcode GGAACTTCAATCACAC-1 contig 1 = ATCAGTCCCA...
umi AACGAACGAC = 600 reads: +388 validated
umi ATCTACAGTC = 310 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 141 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [0, 3]
of which 2 are surviving nonsolos
reads assigned: 896
start codons at 23, 29, 98, 234, 453
confident = true

REJECT CONTIGS

TIG 1[bases=383]
15-136 ==> 231-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=7)
134-172 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
172-383 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
cdr3 = CSAWDSSLNVWVF at 105, score = 7 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 946
start codons at 113, 130
confident = false
not full
VJ delta = 21
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1672 = GGAACTTCACGTGAGA-1

using 10548 reads

====================================================================================

graph has 4558 edges initially, 46 edges after simplification

total ucounts = 646
nonsolo ucounts = 294[2^109, 3^51, 4^33, 5^20, 6^10, 7^6, 8^10, 9^5, 10, 11^4, 12^2, 14^2, 15, 18, 19, 27, 35, 43, 65, 89, 97, 118, 119, 134, 169, 177, 188, 197, 201, 207, 213, 215, 216, 219, 233, 234, 238, 241, 242, 243, 244, 257, 266, 279, 280, 282, 288, 296, 299^2, 460, 851, 947]
surviving nonsolo ucounts = 37[11, 35, 65, 89, 97, 118, 119, 134, 169, 177, 188, 197, 201, 207, 213, 215, 216, 219, 233, 234, 238, 241, 242, 243, 244, 257, 266, 279, 280, 282, 288, 296, 299^2, 460, 851, 947]
ids = [319, 425, 161, 120, 206, 463, 218, 370, 404, 127, ...]

====================================================================================

UMI info for barcode GGAACTTCACGTGAGA-1 contig 1 = GGGGTCTCAG...
umi ACCTCCCACA = 267 reads: +388 validated
umi ATCTACTGTC = 220 reads: +388 validated
umi CAAAAAGACG = 288 reads: +388 validated
umi CAACAGGGTT = 184 reads: +388 validated
umi CACGCCTGCA = 177 reads: +388 validated
umi CAGCATCATG = 946 reads: -210X +178 invalidated
umi CATTGCGGAA = 310 reads: +106 -1X +281 invalidated
umi CCAGCGTCTA = 244 reads: +388 validated
umi GCATCGTTGG = 215 reads: +388 validated
umi GCCAAATTTC = 245 reads: +388 validated
umi GCTTCATCAT = 281 reads: +388 validated
umi GTCTACTCCT = 173 reads: +388 validated
umi TAGCAGAGTA = 118 reads: +388 validated
umi TAGCGCAATT = 217 reads: +388 validated
umi TATGCTAGGC = 244 reads: +388 validated
umi TCACTCTCTG = 262 reads: +388 validated
umi TTGTGTTCCT = 216 reads: +388 validated

UMI info for barcode GGAACTTCACGTGAGA-1 contig 2 = GAGCTCTGGG...
umi AGTTGTACCC = 236 reads: +426 -1 non-validated
umi CATTTCCCCT = 64 reads: +323 -1 +1 -1 +22 -45 +2 -1 +4 -1 +13 -1 +12 non-validated
umi CCTTGTCACT = 99 reads: +427 validated
umi CTGTCATTTT = 859 reads: -192 +1 -1X +233 invalidated
umi CTGTGCCGTA = 240 reads: +427 validated
umi GGACTACCCA = 269 reads: +427 validated
umi GTTATCCGGA = 278 reads: +420 -1 +1 -1 +1 -1 +2 non-validated
umi GTTATTGCAC = 34 reads: +349 -1 +5 -72 non-validated
umi TACTTCATGT = 194 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=637]
0-38 ==> 125-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
38-392 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=1)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
426-637 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 17 umis using 772 reads
cdr3 = CSSYAGSNNCLF at 362, score = 8 + 8
umis assigned: [21, 87, 112, 114, 127, 136, 158, 170, 340, 341] and 7 others
of which 17 are surviving nonsolos
reads assigned: 4519
start codons at 38, 195, 239, 246, 345, 372
confident = true

TIG 2[bases=578]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=2)
444-507 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
507-578 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 4 umis using 173 reads
cdr3 = CARDLRPYNYYYYGMDVW at 422, score = 9 + 7
umis assigned: [63, 161, 206, 292, 293, 364, 423, 425, 458]
of which 9 are surviving nonsolos
reads assigned: 2241
start codons at 80, 231, 236, 289, 294, 297, 315, 383, 464
confident = true

REJECT CONTIGS

TIG 1[bases=774]
3-155 ==> 1073-1225 on segment before IGLV5-37 exon 1 [len=1225] (mis=0)
160-529 ==> 0-369 on |376|IGLV5-37|L-REGION+V-REGION| [len=369] (mis=3)
525-563 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
563-774 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
umis assigned: [120, 132, 185, 215, 218, 244, 357, 370, 457]
of which 9 are surviving nonsolos
reads assigned: 1783
start codons at 57, 73, 98, 141, 160, 302, 434, 485, 505, 521
confident = false
did not find CDR3
now this is a cell
paired!

ACGGCTGTGTATTACTGTGCGAGAGATTTGAGACCCTACAACTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> TCTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCAGCTCATATGCAGGCAGCAACAATTGCCTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1674 = GGAACTTCAGACTCGC-1

using 93 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[91]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1683 = GGAACTTCAGTCAGCC-1

using 440 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 435]
surviving nonsolo ucounts = 1[435]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=482]
0-236 ==> 117-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
233-271 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
271-482 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CASWDDSLRGRVF at 204, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 432
start codons at 37, 187, 212, 217
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1688 = GGAACTTCATCCAACA-1

using 132 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 125]
surviving nonsolo ucounts = 1[125]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1690 = GGAACTTCATCGGGTC-1

using 274 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 266]
surviving nonsolo ucounts = 1[266]
ids = [5]

====================================================================================

UMI info for barcode GGAACTTCATCGGGTC-1 contig 1 = GAGGAGTCAG...
umi TTCATTTCTC = 265 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=549]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=5)
375-413 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQSYSTVTF at 355, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 28, 34, 90, 103, 239, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1692 = GGAACTTCATGACGGA-1

using 406 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[406]
surviving nonsolo ucounts = 1[406]
ids = [0]

====================================================================================

UMI info for barcode GGAACTTCATGACGGA-1 contig 1 = GAGGAACTGC...
umi TATAATGGGC = 356 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=496]
0-33 ==> 64-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
33-378 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=14)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
415-496 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CHQYDNWPQTF at 354, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 351
start codons at 33, 82, 102, 238, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1695 = GGAACTTCATTTCAGG-1

using 265 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[4^2, 250]
surviving nonsolo ucounts = 1[250]
ids = [5]

====================================================================================

UMI info for barcode GGAACTTCATTTCAGG-1 contig 1 = TCAGTCCCAC...
umi CGTTAAGGAT = 240 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=491]
0-22 ==> 5-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
22-373 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
372-410 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
410-491 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CLQYSSSPWTF at 349, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 22, 28, 97, 233, 452
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1701 = GGAACTTGTACTTAGC-1

using 14271 reads

====================================================================================

graph has 5050 edges initially, 56 edges after simplification

total ucounts = 730
nonsolo ucounts = 342[2^120, 3^72, 4^39, 5^21, 6^11, 7^8, 8^5, 9^2, 10^3, 12^2, 14^2, 17, 27, 45, 51, 57, 58, 61, 63, 75, 76, 87, 93, 97, 102, 117, 122, 129, 151, 158, 162, 174, 176, 192, 195, 198, 200, 202, 208, 220, 224, 234, 249, 255, 271, 276, 283, 284, 295, 296, 297, 300, 305, 307, 310, 319, 320, 335, 338, 343, 346, 349, 351, 374, 407, 498, 555, 670]
surviving nonsolo ucounts = 51[51, 57, 58, 61, 63, 75, 93, 97, 102, 117, 122, 129, 151, 158, 162, 174, 176, 192, 195, 198, 200, 202, 208, 220, 224, 234, 249, 255, 271, 276, 283, 284, 295, 296, 297, 300, 305, 307, 310, 319, 320, 335, 338, 343, 349, 351, 374, 407, 498, 555, 670]
ids = [81, 413, 184, 435, 331, 721, 490, 62, 597, 609, ...]

====================================================================================

UMI info for barcode GGAACTTGTACTTAGC-1 contig 1 = AGCTCTGAGA...
umi AACCGCCATA = 322 reads: +415 validated
umi ACGCCCTGGC = 47 reads: +324 -1 +17 -73 non-validated
umi CTAAACCCTA = 65 reads: +319 -3 +85 -8 non-validated
umi CTTCAGCGGA = 497 reads: -145 +270 non-validated
umi TAGACTTTCC = 194 reads: +415 validated
umi TAGTGTATGC = 159 reads: +415 validated
umi TGCAGCACAT = 121 reads: +415 validated
umi TGCGGGGGAT = 188 reads: +408 -1X +1 -5X invalidated
umi TGGCGACACC = 239 reads: +415 validated

UMI info for barcode GGAACTTGTACTTAGC-1 contig 2 = GGGAGAGCCC...
umi AAAGTTATAG = 177 reads: +385 validated
umi AAATAATACC = 411 reads: +385 validated
umi AATGCACCAA = 308 reads: +385 validated
umi ACACTGTTTC = 99 reads: +385 validated
umi ACCATTCCTT = 220 reads: +385 validated
umi ACCTTCGGCT = 338 reads: +385 validated
umi AGCTTTCTTC = 300 reads: +385 validated
umi AGGTTTTATC = 176 reads: +385 validated
umi AGTTCTTCAT = 306 reads: +385 validated
umi ATGTTAGGGT = 122 reads: -337 +2 -5XX +1 -2XX +1 -2XX +6 -1XX +3 -3XX +9 -1XX +12 invalidated
umi ATTAATCAAC = 59 reads: +385 validated
umi ATTCATAGTA = 332 reads: +385 validated
umi CAACACTTTC = 275 reads: +385 validated
umi CACAGATCGA = 693 reads: +385 validated
umi CACGTGCCCG = 557 reads: +385 validated
umi CATTCGATGT = 205 reads: +385 validated
umi CCCATTGGCC = 221 reads: +385 validated
umi CCCCCTCTTT = 192 reads: +385 validated
umi CCCCGTTTGC = 287 reads: +385 validated
umi CCCTTGAATT = 203 reads: +385 validated
umi CCTTTAATAC = 297 reads: +385 validated
umi CGCCTCGCTG = 298 reads: +385 validated
umi CGGCTTCGCA = 125 reads: +385 validated
umi CTATCTTTCG = 304 reads: +385 validated
umi CTGCACCGGG = 235 reads: +385 validated
umi GATCTGGCGC = 257 reads: +385 validated
umi GCACCTGACA = 57 reads: +385 validated
umi GCATCTGGAG = 200 reads: +385 validated
umi GCCGTGTTAG = 131 reads: +385 validated
umi GCCTCGTCAG = 163 reads: +385 validated
umi GCTATTGCAA = 61 reads: +385 validated
umi TAACACTAAC = 93 reads: +385 validated
umi TAGCAACAGG = 149 reads: +385 validated
umi TCTGGAACCG = 354 reads: -23X +362 invalidated
umi TCTTTTTTGC = 103 reads: +385 validated
umi TGCATAAGAT = 336 reads: +385 validated
umi TGTTCAGCAC = 317 reads: +385 validated
umi TTACGTGGCT = 293 reads: +385 validated
umi TTCAACTGCT = 350 reads: +385 validated
umi TTTCAACCTA = 274 reads: +385 validated
umi TTTCGGCCAA = 370 reads: +385 validated
umi TTTTTGGGTC = 282 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=565]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=5)
446-494 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
494-565 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 5 umis using 112 reads
cdr3 = CATSITVVTYFDYW at 421, score = 9 + 7
umis assigned: [17, 81, 331, 364, 520, 525, 609, 614, 623]
of which 9 are surviving nonsolos
reads assigned: 1794
start codons at 79, 230, 235, 382
confident = true

TIG 2[bases=568]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
395-432 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 41 umis using 1657 reads
cdr3 = CQQRSNWPPLTF at 368, score = 9 + 9
umis assigned: [7, 8, 49, 62, 65, 79, 115, 128, 135, 179] and 32 others
of which 41 are surviving nonsolos
reads assigned: 10368
start codons at 47, 252, 255, 474
confident = true
now this is a cell
paired!

AGAGCCGAGGACACGGCCGTATATTACTGTGCGACCAGCATAACGGTGGTAACTTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1706 = GGAACTTGTATCGCAT-1

using 150 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[150]
surviving nonsolo ucounts = 1[150]
ids = [0]

====================================================================================

UMI info for barcode GGAACTTGTATCGCAT-1 contig 1 = GGCTTTCTGA...
umi CGTATTTTGT = 149 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=576]
15-386 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
403-451 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
451-576 ==> 0-125 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 22 reads
cdr3 = CARYFAFVNYYFDKW at 375, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 145
start codons at 15, 36, 80
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1710 = GGAACTTGTCATGCAT-1

using 435 reads

====================================================================================

graph has 217 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3^2, 190, 233]
surviving nonsolo ucounts = 2[190, 233]
ids = [1, 0]

====================================================================================

UMI info for barcode GGAACTTGTCATGCAT-1 contig 1 = ACTGGTTGTT...
umi ACGTTTTTTG = 236 reads: +406 validated

GOOD CONTIGS

TIG 1[bases=561]
0-110 ==> 65-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
110-473 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
478-516 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
516-561 ==> 0-45 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 8 reads
cdr3 = CQQYYSTPPLFTF at 449, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 230
start codons at 110, 179, 432
confident = false

REJECT CONTIGS

TIG 1[bases=520]
1-341 ==> 1146-1486 on rc of segment before IGHD5-12 exon 1 [len=1486] (mis=0)
368-418 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
418-520 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 51, 66, 90, 399, 436, 497
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1712 = GGAACTTGTCCTCCAT-1

using 311 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 304]
surviving nonsolo ucounts = 1[304]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=479]
1-82 ==> 5665-5746 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=3)
25-378 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=1)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-479 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 25, 31, 87, 100, 236, 453
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1721 = GGAACTTGTGTGTGCC-1

using 255 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 4, 242]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1724 = GGAACTTGTTAAGTAG-1

using 397 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[395]
surviving nonsolo ucounts = 1[395]
ids = [0]

====================================================================================

UMI info for barcode GGAACTTGTTAAGTAG-1 contig 1 = GAGTCAGTCT...
umi CCGATATACC = 395 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=543]
25-378 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=4)
369-407 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
407-543 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 71 reads
cdr3 = CQQSYSTSF at 352, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 388
start codons at 25, 31, 87, 100, 236, 449
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1725 = GGAACTTGTTAGAACA-1

using 24320 reads

====================================================================================

graph has 11822 edges initially, 112 edges after simplification

total ucounts = 2147
nonsolo ucounts = 1081[2^355, 3^233, 4^133, 5^94, 6^69, 7^31, 8^29, 9^15, 10^7, 11^6, 12^10, 13^3, 14^2, 17^2, 18, 19, 20, 22, 29, 33, 38, 49, 53, 55, 61, 65, 71, 72^2, 79, 83, 84, 87, 88, 89, 91, 95, 104, 105, 109, 131, 134, 137, 140, 143, 146, 154^2, 158^2, 166, 168, 169, 172, 178, 181, 187^2, 189, 197, 201, 202, 212, 225, 233, 235, 237, 240, 248^2, 249, 256, 268, 269, 270, 275, 276, 282, 287, 289, 295, 296, 299, 301, 308, 311, 316^2, 319, 321^2, 324, 331, 334, 341, 345^2, 357, 359, 369, 376, 413, 521, 532, 560, 636]
surviving nonsolo ucounts = 83[33, 38, 49, 53, 55, 61, 65, 71, 72^2, 79, 83, 84, 87, 88, 89, 91, 105, 131, 134, 140, 143, 146, 154^2, 158^2, 166, 168, 169, 172, 178, 181, 187^2, 189, 197, 201, 202, 212, 225, 233, 235, 237, 240, 248^2, 249, 256, 268, 269, 270, 275, 276, 282, 287, 289, 295, 296, 299, 301, 308, 311, 316^2, 319, 321^2, 324, 331, 334, 341, 345^2, 357, 359, 369, 376, 413, 521, 532, 560, 636]
ids = [152, 1944, 370, 1573, 1419, 1087, 355, 1296, 179, 202, ...]

====================================================================================

UMI info for barcode GGAACTTGTTAGAACA-1 contig 1 = GGAGGAGTCA...
umi AAACGGAGCG = 315 reads: +388 validated
umi AACTGGCACC = 355 reads: +388 validated
umi ACAATGATCC = 318 reads: +388 validated
umi ACCCTTTATG = 267 reads: +388 validated
umi ACGTAGCCAC = 330 reads: +388 validated
umi ACTACATTTT = 309 reads: +388 validated
umi ACTGTCTCAC = 322 reads: +388 validated
umi AGGACATATC = 504 reads: -346X +1 -3XX +2 -2XX +2 -1XX +2 -1XX +5 -1XX +9 -1XX +4 -1XX +7 invalidated
umi AGTACTACGC = 327 reads: +388 validated
umi ATTTAGTAAC = 179 reads: +388 validated
umi CATAAACCTG = 341 reads: +388 validated
umi CATCAATCTG = 356 reads: +388 validated
umi CATCGCACTT = 414 reads: +56 -2XX +1 -6XX +3 -7XX +2 -297XX +3 -3XX +1 -1XX +2 -2XX +1 -1XX invalidated
umi CCCACACTTC = 280 reads: +388 validated
umi CCTTCCTATC = 315 reads: +388 validated
umi CGCCCAGCCC = 292 reads: +127 -1XX +260 invalidated
umi CTTATCACTA = 319 reads: +388 validated
umi CTTCATCAGT = 520 reads: +388 validated
umi GAACTACTTG = 255 reads: +388 validated
umi GAGCGCATCC = 303 reads: +388 validated
umi GATGACCCTT = 249 reads: +388 validated
umi GATTTCCCAC = 225 reads: +388 validated
umi GCCGTAGCAT = 302 reads: +388 validated
umi GTGCATTTTA = 296 reads: +388 validated
umi GTTTAGCCTT = 302 reads: +388 validated
umi GTTTATGGAG = 322 reads: +388 validated
umi TCAGCCTCCT = 238 reads: +388 validated
umi TCCCAGATGG = 308 reads: +388 validated
umi TCGCAGCCGG = 288 reads: +388 validated
umi TCTTAAGGAA = 271 reads: +388 validated
umi TGACAATATG = 285 reads: +388 validated
umi TGACACTTGC = 345 reads: +388 validated
umi TGCATAATCA = 367 reads: +388 validated
umi TGGACCATTG = 379 reads: +388 validated
umi TGGCAGCATT = 159 reads: +388 validated
umi TTACTCATTA = 176 reads: +388 validated
umi TTCAACCGAT = 232 reads: +388 validated
umi TTGTCCTAAA = 340 reads: -350X +2 -2XX +2 -1XX +2 -1XX +5 -1XX +9 -1XX +4 -1XX +7 invalidated
umi TTTCGTCTTA = 638 reads: +388 validated
umi TTTGACCCGT = 146 reads: +388 validated
umi TTTGAGTGAA = 266 reads: +388 validated
umi TTTTCTCTCC = 241 reads: +388 validated

UMI info for barcode GGAACTTGTTAGAACA-1 contig 2 = GGAGTCTCCC...
umi AAAGTTACCT = 200 reads: +421 validated
umi AACGGGCGTT = 147 reads: +421 validated
umi ACAAGTGCCG = 34 reads: +332 -5 +1 -1 +4 -1 +49 -3 +25 non-validated
umi ACAGTTTTCC = 72 reads: +402 -19 non-validated
umi ACCATCAGCT = 73 reads: +421 validated
umi ACCCTCACAC = 78 reads: +350 -1 +1 -16 +53 non-validated
umi AGAATCTCTG = 154 reads: +421 validated
umi AGTACCTCTG = 64 reads: +421 validated
umi AGTCCTTATT = 48 reads: +410 -11 non-validated
umi AGTCTTGCCG = 166 reads: +421 validated
umi ATCACTATTC = 211 reads: +421 validated
umi ATCATGTTTT = 88 reads: +413 -8 non-validated
umi ATCCTTCTTA = 155 reads: +421 validated
umi ATTAGACCAC = 188 reads: +421 validated
umi CACGACCATC = 250 reads: +418 -1 +2 non-validated
umi CACTATTTTC = 203 reads: +421 validated
umi CATCGCTCAT = 189 reads: +421 validated
umi CCCAGCACTA = 141 reads: +421 validated
umi CCTACACACC = 88 reads: +108 -1XX +194 -1 +3 -1 +2 -1 +7 -1 +4 -1 +7 -1X +11 -1 +4 -1 +2 -2 +4 -1 +1 -1 +1 -2 +5 -1 +3 -1 +1 -1 +7 -6 +1 -3 +5 -1 +1 -1 +9 -1 +11 invalidated
umi CGCTATTCCT = 87 reads: +288 -2 +6 -2 +123 non-validated
umi CGTATCAGGC = 551 reads: +70 -2XX +1 -2XX +2 -18XX +1 -325 invalidated
umi CGTTCAGCCA = 274 reads: +9 -7XX +1 -1XX +1 -3XX +1 -1XX +1 -2XX +1 -355X +1 -2XX +2 -4XX +3 -9XX +17 invalidated
umi CTACTTTTCG = 145 reads: +421 validated
umi CTCCGCGCAT = 62 reads: +421 validated
umi GAATCCTTAT = 103 reads: +421 validated
umi GCACCAAGGG = 68 reads: +421 validated
umi GCACCCCCAC = 133 reads: +421 validated
umi GCGCAACATC = 87 reads: +421 validated
umi GGTTGATATG = 56 reads: +381 -40 non-validated
umi GTCACTAATA = 186 reads: +402 -1 +18 non-validated
umi GTTCCATAAC = 204 reads: +421 validated
umi TACATTTCCT = 51 reads: +421 validated
umi TAGGTAACGG = 183 reads: +421 validated
umi TATACCGGCT = 83 reads: +404 -17 non-validated
umi TCATAGGGAT = 171 reads: +421 validated
umi TCATGAATAT = 168 reads: +421 validated
umi TCGTTTGCAC = 91 reads: +384 -37 non-validated
umi TTAATTAGCC = 39 reads: +296 -1 +28 -10 +79 -7 non-validated
umi TTTCAGTATC = 232 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=553]
0-29 ==> 0-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=1)
29-380 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=17)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 39 umis using 1885 reads
cdr3 = CLQDYNFPFTF at 356, score = 9 + 8
umis assigned: [15, 74, 161, 213, 244, 253, 272, 334, 356, 547] and 32 others
of which 42 are surviving nonsolos
reads assigned: 12792
start codons at 29, 35, 91, 459
confident = true

TIG 2[bases=662]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=25)
430-480 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=6)
480-662 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 27 umis using 383 reads
cdr3 = CARSQRLNWGGAFHIG at 401, score = 8 + 7
umis assigned: [30, 63, 152, 179, 202, 210, 289, 355, 370, 374] and 29 others
of which 39 are surviving nonsolos
reads assigned: 5405
start codons at 59, 233, 257, 323, 392, 461
confident = true
now this is a cell
paired!

TCGGACACCGCCATGTATTACTGTGCGAGGTCCCAGAGGCTGAACTGGGGTGGTGCTTTTCATATTGGGGGCCAAGGGACAATGGTCACTGTGTCTTCAG <==> ATCAGCAGCGTGCAGCCTGAAGATTTTGCAACTTATTACTGTCTACAAGATTATAATTTCCCGTTCACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1736 = GGAACTTTCACATACG-1

using 115 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 14[2^5, 3^2, 4^2, 7, 9, 11^2, 46]
surviving nonsolo ucounts = 1[46]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1738 = GGAACTTTCACCATAG-1

using 224 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 220]
surviving nonsolo ucounts = 1[220]
ids = [2]

====================================================================================

UMI info for barcode GGAACTTTCACCATAG-1 contig 1 = GCTCTGCTTC...
umi TCATTTAGCA = 213 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=503]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-503 ==> 0-58 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1743 = GGAACTTTCATACGGT-1

using 247 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 6, 230]
surviving nonsolo ucounts = 1[230]
ids = [5]

====================================================================================

UMI info for barcode GGAACTTTCATACGGT-1 contig 1 = GGACTGATCA...
umi CGACGCGGAT = 221 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=476]
35-395 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
394-432 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
432-476 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CMQALQTPVTF at 371, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 220
start codons at 35, 68, 104, 192, 354, 374
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1748 = GGAACTTTCCACGTGG-1

using 627 reads

====================================================================================

graph has 170 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 9[2, 3^2, 4^2, 5, 6, 276, 322]
surviving nonsolo ucounts = 2[276, 322]
ids = [6, 5]

====================================================================================

UMI info for barcode GGAACTTTCCACGTGG-1 contig 1 = GCTCTGCTTC...
umi CGCGCATCTC = 273 reads: +394 validated

UMI info for barcode GGAACTTTCCACGTGG-1 contig 2 = GTCAGTCTCA...
umi CCTTGGGTAT = 304 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=596]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-596 ==> 0-151 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false

TIG 2[bases=461]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
376-408 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=0)
408-461 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 72 reads
cdr3 = CQQSYSTPVF at 350, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 302
start codons at 23, 29, 85, 98, 234, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1749 = GGAACTTTCCACGTTC-1

using 157 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[156]
surviving nonsolo ucounts = 1[156]
ids = [0]

====================================================================================

UMI info for barcode GGAACTTTCCACGTTC-1 contig 1 = GGGGATGCTT...
umi TGAGCACCAG = 151 reads: +451 validated

GOOD CONTIGS

TIG 1[bases=552]
20-397 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=3)
423-471 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=0)
471-552 ==> 0-81 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 19 reads
cdr3 = CARHMGRYGDFLYYFDYW at 386, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 151
start codons at 4, 20, 29, 41, 85, 398
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 71.1750 = GGAACTTTCCAGAAGG-1

using 285 reads

====================================================================================

graph has 106 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 22, 257]
surviving nonsolo ucounts = 1[257]
ids = [6]

====================================================================================

UMI info for barcode GGAACTTTCCAGAAGG-1 contig 1 = GGGGTCACAA...
umi TTGGCATTTT = 252 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=527]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=6)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
432-527 ==> 0-95 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CCSYTGSSTTYVVF at 362, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 38, 177, 239, 246, 393
confident = false
NOT paired!

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BARCODE 71.1751 = GGAACTTTCCGAATGT-1

using 52 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 47]
surviving nonsolo ucounts = 1[47]
ids = [0]

====================================================================================
NOT paired!

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BARCODE 71.1762 = GGAACTTTCTACTCAT-1

using 16242 reads

====================================================================================

graph has 9377 edges initially, 88 edges after simplification

total ucounts = 1702
nonsolo ucounts = 884[2^309, 3^202, 4^131, 5^75, 6^34, 7^27, 8^10, 9^11, 10^13, 11^4, 12, 14, 15^3, 17, 18, 22^2, 23, 26, 43, 51, 52, 66, 71, 72, 74^2, 108^2, 109, 114, 117, 123, 142, 174, 176, 181, 185, 192, 201, 202, 204, 205, 208, 215^4, 219, 220, 228, 234^2, 240, 241, 243, 249, 258, 260, 261, 271, 282, 284, 287, 289, 292, 302, 310, 311, 312^2, 316, 335, 342, 518, 594]
surviving nonsolo ucounts = 58[14, 22, 43, 51, 52, 66, 71, 72, 74^2, 108^2, 109, 114, 117, 123, 142, 174, 176, 181, 185, 192, 201, 202, 204, 205, 208, 215^4, 219, 220, 228, 234^2, 240, 241, 243, 249, 258, 260, 261, 271, 282, 284, 287, 289, 292, 302, 310, 311, 312^2, 316, 335, 342, 518]
ids = [349, 805, 1349, 1354, 1391, 735, 1449, 1672, 708, 1035, ...]

====================================================================================

UMI info for barcode GGAACTTTCTACTCAT-1 contig 1 = AGGTCTCAGA...
umi AAATATATAT = 111 reads: +427 validated
umi AGGCCGCGTC = 337 reads: +427 validated
umi AGTAACACGG = 107 reads: +427 validated
umi ATGTCGCACG = 112 reads: +423 -4 non-validated
umi CATTAAAACG = 220 reads: +427 validated
umi CCCTCCGGCA = 249 reads: +427 validated
umi CCTAAATGCC = 299 reads: +427 validated
umi CCTACAATCC = 315 reads: +427 validated
umi CCTTTATCGG = 122 reads: +427 validated
umi CGCCTAGCCT = 261 reads: +427 validated
umi CGTCAGTATC = 196 reads: +427 validated
umi CGTCTACTAG = 220 reads: +427 validated
umi CTAATACCAC = 114 reads: +421 -6 non-validated
umi CTGGTCCCTA = 202 reads: +427 validated
umi GACCACACCT = 159 reads: +83 -1XX +3 -1 +3 -1XX +2 -2XX +2 -8XX +321 invalidated
umi GACGAATGCA = 187 reads: +420 -7 non-validated
umi GCTACGCTGG = 74 reads: +395 -1 +2 -29 non-validated
umi GGGCGCATTA = 220 reads: +427 validated
umi GTGCTCCAAC = 247 reads: +373 -1 +16 -31 +6 non-validated
umi GTTTTCACCC = 309 reads: +304 -1XX +122 invalidated
umi GTTTTGCCTT = 177 reads: +391 -1 +15 -1 +1 -4 +14 non-validated
umi TAAATGGTCT = 192 reads: +412 -15 non-validated
umi TATTGCGGAA = 200 reads: +427 validated
umi TCACTTATTT = 208 reads: +427 validated
umi TCCTATATCG = 179 reads: +425 -2 non-validated
umi TCGTTGACCT = 140 reads: +404 -1 +16 -6 non-validated
umi TCTTCTGCAT = 70 reads: +427 validated
umi TGCCGTCTTA = 527 reads: -107X +3 -7XX +1 -2XX +2 -2XX +303 invalidated
umi TGCTCCGATT = 106 reads: +398 -29 non-validated
umi TTAATCCCCG = 255 reads: +427 validated
umi TTATACAGCG = 234 reads: +399 -1 +7 -2 +3 -1 +1 -1 +2 -1 +9 non-validated
umi TTCACCGCAC = 213 reads: +427 validated

UMI info for barcode GGAACTTTCTACTCAT-1 contig 2 = GGGAGAGCCC...
umi ACACTAAGTC = 281 reads: +382 validated
umi ACTGCCCTCT = 240 reads: +382 validated
umi ATCAGTCCCT = 315 reads: +382 validated
umi ATTACCTCCT = 213 reads: +382 validated
umi CATTCCTCTT = 285 reads: +382 validated
umi CCTACACGTT = 215 reads: +382 validated
umi CGTGGGAGAC = 73 reads: +380 -1 +1 non-validated
umi CTACCTGCAA = 229 reads: -341X +1 -7XX +1 -1XX +7 -2XX +8 -1XX +5 -2XX +6 invalidated
umi GACTATGTGT = 321 reads: +382 validated
umi GCCAATACCG = 294 reads: +382 validated
umi GCGTTATGTT = 247 reads: +382 validated
umi GCTGTTACAT = 206 reads: +382 validated
umi TACTAAGACA = 312 reads: +382 validated
umi TATCCCCAAA = 229 reads: +382 validated
umi TATGTCCCTC = 271 reads: +382 validated
umi TCAGTATCAT = 300 reads: +382 validated
umi TCGGTCACGG = 290 reads: +382 validated
umi TCTGTTGGCC = 345 reads: +382 validated
umi TTCCCGGCCG = 236 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=577]
0-79 ==> 0-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=1)
79-432 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=11)
458-506 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
506-577 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 22 umis using 374 reads
cdr3 = CARGRYYGSGTYYGFDYW at 421, score = 8 + 7
umis assigned: [24, 222, 236, 291, 449, 532, 566, 571, 615, 659] and 22 others
of which 32 are surviving nonsolos
reads assigned: 6417
start codons at 79, 235, 296, 314, 382, 440, 458
confident = true

TIG 2[bases=565]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=9)
391-429 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=5)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 18 umis using 740 reads
cdr3 = CQQRNNWLPAF at 368, score = 9 + 7
umis assigned: [95, 186, 265, 297, 459, 572, 708, 732, 925, 986] and 9 others
of which 18 are surviving nonsolos
reads assigned: 4831
start codons at 47, 252, 255, 420, 471
confident = true
now this is a cell
paired!

ACGGCTGTTTATTACTGTGCGAGAGGTCGGTACTATGGTTCAGGGACTTATTATGGCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAACAACTGGCTTCCCGCTTTCGGCCCTGGGACCAAAGTGGATGTCAAAC

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BARCODE 71.1763 = GGAACTTTCTATCGCC-1

using 19 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 71.1764 = GGAACTTTCTCGTATT-1

using 2595 reads

====================================================================================

graph has 1138 edges initially, 18 edges after simplification

total ucounts = 272
nonsolo ucounts = 84[2^31, 3^17, 4^8, 5^8, 6^5, 7^3, 9, 10^3, 11, 25, 149, 156, 165, 206, 229, 1190]
surviving nonsolo ucounts = 5[156, 165, 206, 229, 1190]
ids = [220, 153, 170, 67, 217]

====================================================================================

UMI info for barcode GGAACTTTCTCGTATT-1 contig 1 = GGCTGTGGGC...
umi CAGCCTGCCT = 224 reads: +382 validated
umi GCCCGCGTCT = 164 reads: +382 validated
umi GGGTCTGGCT = 204 reads: +382 validated
umi TCTAGGCTTT = 154 reads: +100 -5XX +277 invalidated

GOOD CONTIGS

TIG 1[bases=572]
0-40 ==> 0-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=1)
40-377 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
422-572 ==> 0-150 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 4 umis using 114 reads
cdr3 = CNSRDSSGNHVVF at 355, score = 8 + 8
umis assigned: [67, 153, 170, 220]
of which 4 are surviving nonsolos
reads assigned: 736
start codons at 40, 159, 188, 239, 338, 383
confident = true
now this is a cell
NOT paired!

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BARCODE 71.1773 = GGAATAAAGACAGAGA-1

using 267 reads

====================================================================================

graph has 362 edges initially, 6 edges after simplification

total ucounts = 124
nonsolo ucounts = 54[2^28, 3^7, 4^9, 5, 6^2, 7^2, 8, 9^2, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.10
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk071-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk071-ucc6a7a72ce/files/contig_bam.bam

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96.456 seconds used processing barcodes, peak mem = 0.26
