[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.03 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk062-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk062-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk062.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.0 = GACTACAAGCCACGTC-1

using 252 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[6, 241]
surviving nonsolo ucounts = 1[241]
ids = [2]

====================================================================================

UMI info for barcode GACTACAAGCCACGTC-1 contig 1 = GAGATCACAG...
umi CCCTATTCTC = 239 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=615]
19-372 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
421-455 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
455-615 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=1)
junction support: 1 umis using 26 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 361, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 19, 217, 222, 239, 283, 316, 509
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.9 = GACTACAAGGGATACC-1

using 225 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[221]
surviving nonsolo ucounts = 1[221]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.11 = GACTACAAGGGTTTCT-1

using 137 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 130]
surviving nonsolo ucounts = 1[130]
ids = [5]

====================================================================================

UMI info for barcode GACTACAAGGGTTTCT-1 contig 1 = AAGAGCTGCT...
umi TCTTCATGGC = 122 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=481]
0-32 ==> 20-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
32-380 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
417-481 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CQQYGSSPVTF at 356, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 119
start codons at 32, 240, 243, 366, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.15 = GACTACAAGTGACATA-1

using 292 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[292]
surviving nonsolo ucounts = 1[292]
ids = [0]

====================================================================================

UMI info for barcode GACTACAAGTGACATA-1 contig 1 = AGTGCTTTCT...
umi AGCGCAGGCA = 252 reads: +445 validated

GOOD CONTIGS

TIG 1[bases=470]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=6)
412-462 ==> 11-61 on |58|IGHJ6|J-REGION| [len=61] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARGGGCSGGSCYYMDVW at 377, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 17, 38, 82, 168, 419
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.17 = GACTACAAGTGGGATC-1

using 271 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2^2, 260]
surviving nonsolo ucounts = 1[260]
ids = [7]

====================================================================================

UMI info for barcode GACTACAAGTGGGATC-1 contig 1 = TGGGAGGAGT...
umi TCTTCCTAAT = 259 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=555]
31-384 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=10)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQSYTTLLTF at 358, score = 9 + 9
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 255
start codons at 31, 37, 93, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.19 = GACTACAAGTGTACGG-1

using 27 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 2[2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.22 = GACTACAAGTTCGCAT-1

using 353 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[351]
surviving nonsolo ucounts = 1[351]
ids = [1]

====================================================================================

UMI info for barcode GACTACAAGTTCGCAT-1 contig 1 = ACCCAAAAAC...
umi GACTATCAGC = 339 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=526]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-526 ==> 0-36 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 336
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.23 = GACTACAAGTTTCCTT-1

using 314 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2^2, 303]
surviving nonsolo ucounts = 1[303]
ids = [6]

====================================================================================

UMI info for barcode GACTACAAGTTTCCTT-1 contig 1 = AGTCAGGACA...
umi GCCCATCCTT = 283 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=464]
14-365 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=5)
364-402 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
402-464 ==> 0-62 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYNSYSRTF at 341, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 279
start codons at 14, 20, 76, 89, 225, 228, 321, 444
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.38 = GACTACACACGAAACG-1

using 611 reads

====================================================================================

graph has 246 edges initially, 36 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^2, 3, 13, 235, 350]
surviving nonsolo ucounts = 2[235, 350]
ids = [8, 5]

====================================================================================

UMI info for barcode GACTACACACGAAACG-1 contig 1 = AGTCTCAGTC...
umi TATAGGGGGG = 232 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=9)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQSYTTLLTF at 347, score = 9 + 9
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 232
start codons at 20, 26, 82, 95, 231, 450
confident = false

REJECT CONTIGS

TIG 1[bases=545]
0-68 ==> 5669-5737 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=2)
20-371 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-409 ==> 10-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
409-545 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 348
start codons at 20, 26, 95, 231, 451
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.45 = GACTACACAGTAGAGC-1

using 485 reads

====================================================================================

graph has 172 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[159, 322]
surviving nonsolo ucounts = 2[159, 322]
ids = [4, 5]

====================================================================================

UMI info for barcode GACTACACAGTAGAGC-1 contig 1 = GGGATCACAC...
umi TTACATATTT = 318 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=571]
0-60 ==> 0-60 on |70|IGHV1-24|5'UTR| [len=60] (mis=4)
60-413 ==> 0-353 on |71|IGHV1-24|L-REGION+V-REGION| [len=353] (mis=20)
433-484 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=1)
484-571 ==> 0-87 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CAIVRFTTASYNWFDPW at 402, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 311
start codons at 60, 205, 216, 258, 280, 324, 357, 538
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.48 = GACTACACAGTTTACG-1

using 12 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.55 = GACTACACATCTATGG-1

using 100 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 1[93]
ids = [7]

====================================================================================

UMI info for barcode GACTACACATCTATGG-1 contig 1 = ACAGCATGGA...
umi TTCATTCTTC = 85 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=403]
5-356 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
356-393 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
junction support: 1 umis using 13 reads
cdr3 = CQQYDNLPPTF at 332, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 84
start codons at 5, 11, 67, 80, 219, 342
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.57 = GACTACACATTGCGGC-1

using 187 reads

====================================================================================

graph has 108 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 19, 162]
surviving nonsolo ucounts = 2[19, 162]
ids = [4, 2]

====================================================================================

UMI info for barcode GACTACACATTGCGGC-1 contig 1 = ACCCAAAAAC...
umi CGAGAAACAC = 160 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=533]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-533 ==> 0-43 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.66 = GACTACAGTAGCCTCG-1

using 83 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[80]
surviving nonsolo ucounts = 1[80]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.68 = GACTACAGTCCGAATT-1

using 336 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 8, 322]
surviving nonsolo ucounts = 1[322]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.74 = GACTACAGTCTCCACT-1

using 319 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 8, 301]
surviving nonsolo ucounts = 1[301]
ids = [1]

====================================================================================

UMI info for barcode GACTACAGTCTCCACT-1 contig 1 = TCTGGCACCA...
umi ATCGGCCACC = 298 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=630]
0-34 ==> 0-34 on |387|IGLV7-46|5'UTR| [len=34] (mis=0)
34-363 ==> 0-329 on |388|IGLV7-46|L-REGION+V-REGION| [len=351] (mis=18)
381-419 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
419-630 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 66 reads
cdr3 = CLIVSGGVVVF at 358, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 296
start codons at 34, 242, 326, 341
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.76 = GACTACAGTCTGCAAT-1

using 362 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^3, 353]
surviving nonsolo ucounts = 1[353]
ids = [5]

====================================================================================

UMI info for barcode GACTACAGTCTGCAAT-1 contig 1 = AACCACATTC...
umi TCCACTTTGG = 317 reads: +442 validated

GOOD CONTIGS

TIG 1[bases=547]
0-48 ==> 10-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
48-401 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=0)
427-490 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=5)
490-547 ==> 0-57 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CARDVLEGRGYDPFFNYYGMDVW at 390, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 311
start codons at 48, 199, 246, 345, 400, 447, 508
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.77 = GACTACAGTCTTGTCC-1

using 847 reads

====================================================================================

graph has 280 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^2, 3, 4, 160, 174, 497]
surviving nonsolo ucounts = 3[160, 174, 497]
ids = [6, 0, 5]

====================================================================================

UMI info for barcode GACTACAGTCTTGTCC-1 contig 1 = AGTCTGGGCC...
umi AAAATTCCGT = 177 reads: +382 validated
umi GAACTTAAGG = 493 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=633]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-373 ==> 0-333 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=29)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-633 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 124 reads
cdr3 = CQVWDSDGHYVVF at 355, score = 8 + 8
umis assigned: [0, 5]
of which 2 are surviving nonsolos
reads assigned: 658
start codons at 40, 101, 170, 338, 374, 383
confident = true

REJECT CONTIGS

TIG 1[bases=440]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-355 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
397-420 ==> 0-23 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
cdr3 = CVAWDDSLYAWVF at 368, score = 8 + 6
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 47, 351, 381, 393
confident = false
not full
VJ delta = 22
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.86 = GACTACAGTGGTACAG-1

using 1463 reads

====================================================================================

graph has 2136 edges initially, 24 edges after simplification

total ucounts = 679
nonsolo ucounts = 302[2^143, 3^62, 4^31, 5^21, 6^17, 7^8, 8^4, 9, 10^5, 12^5, 13^2, 14, 16, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.96 = GACTACAGTTCAGGCC-1

using 325 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[3, 316]
surviving nonsolo ucounts = 1[316]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=515]
0-77 ==> 5533-5610 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=2)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=0)
390-429 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
429-515 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CWGLLLHARYTLASDTF at 350, score = 3 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 30, 63, 91, 99, 187, 349, 369, 471
confident = false
not full
frameshifted full length transcript of length 515
VJ delta = 31
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.101 = GACTACAGTTTACTCT-1

using 285 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2^2, 3^2, 267]
surviving nonsolo ucounts = 1[267]
ids = [0]

====================================================================================

UMI info for barcode GACTACAGTTTACTCT-1 contig 1 = GTCAGACTCA...
umi AAGGGTCTTC = 258 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=472]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
411-472 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQQYNSYPLTF at 350, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 23, 29, 85, 98, 234, 237, 330, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.103 = GACTACATCAACACGT-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.112 = GACTACATCCGTAGTA-1

using 585 reads

====================================================================================

graph has 887 edges initially, 6 edges after simplification

total ucounts = 298
nonsolo ucounts = 111[2^43, 3^31, 4^17, 5^6, 6^3, 7^3, 8^2, 10^2, 11^3, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.122 = GACTACATCGGTCCGA-1

using 155 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 4[2^3, 141]
surviving nonsolo ucounts = 1[141]
ids = [4]

====================================================================================

UMI info for barcode GACTACATCGGTCCGA-1 contig 1 = CTGGGGTCTC...
umi CATTTTTCGG = 139 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=444]
40-362 ==> 0-322 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=9)
393-431 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
junction support: 1 umis using 24 reads
cdr3 = CCSFTVNTRSYVF at 364, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 137
start codons at 40, 197, 241, 251, 395
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.132 = GACTACATCTGGCGTG-1

using 57 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[54]
surviving nonsolo ucounts = 1[54]
ids = [2]

====================================================================================

UMI info for barcode GACTACATCTGGCGTG-1 contig 1 = CTTCAGCTGT...
umi TAGCCCACTA = 55 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=486]
0-45 ==> 69-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
45-398 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
433-486 ==> 0-53 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CASWDDSLRGRVF at 366, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 54
start codons at 45, 199, 349, 374, 379
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.134 = GACTACATCTTCGAGA-1

using 205 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 199]
surviving nonsolo ucounts = 1[199]
ids = [0]

====================================================================================

UMI info for barcode GACTACATCTTCGAGA-1 contig 1 = GCTCTGCTTC...
umi AGGGACCGTT = 197 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=567]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-567 ==> 0-122 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 196
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.137 = GACTGCGAGACTAAGT-1

using 233 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 4, 5, 14, 206]
surviving nonsolo ucounts = 1[206]
ids = [3]

====================================================================================

UMI info for barcode GACTGCGAGACTAAGT-1 contig 1 = CACAAGAGGC...
umi CTAAGGTGCC = 193 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=531]
0-33 ==> 129-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
33-373 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=11)
380-418 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
418-531 ==> 0-113 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CCSKAGRSYVF at 357, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 192
start codons at 33, 172, 241, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.145 = GACTGCGAGCCTATGT-1

using 100 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 27
nonsolo ucounts = 17[2^8, 3^2, 4, 5, 7, 10^2, 12, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.147 = GACTGCGAGGACTGGT-1

using 244 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[244]
surviving nonsolo ucounts = 1[244]
ids = [0]

====================================================================================

UMI info for barcode GACTGCGAGGACTGGT-1 contig 1 = GGGGTCACAA...
umi ATAGGCTGAC = 250 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=643]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
432-643 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 37 reads
cdr3 = CCSEAGGGVPGLLF at 362, score = 7 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 242
start codons at 38, 192
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.150 = GACTGCGAGGCCCTCA-1

using 197 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 6[2^2, 3, 4, 11, 168]
surviving nonsolo ucounts = 1[168]
ids = [10]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.152 = GACTGCGAGGGCTCTC-1

using 134 reads

====================================================================================

graph has 148 edges initially, 4 edges after simplification

total ucounts = 29
nonsolo ucounts = 17[2^5, 3, 4, 5^4, 6, 7, 9, 12, 22, 29]
surviving nonsolo ucounts = 1[29]
ids = [9]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 62.154 = GACTGCGAGGTGCACA-1

using 175 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 171]
surviving nonsolo ucounts = 1[171]
ids = [1]

====================================================================================

UMI info for barcode GACTGCGAGGTGCACA-1 contig 1 = GGGGTCTCAG...
umi CACTTACAGT = 160 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=473]
0-38 ==> 3-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=0)
38-381 ==> 0-343 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=18)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
429-473 ==> 0-44 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CSSYTSISTFVVF at 362, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 157
start codons at 38, 189, 246
confident = false
NOT paired!
sorting bam, mem = 0.03
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk062-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk062-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

2.079 seconds used processing barcodes, peak mem = 0.23
