[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 833.97 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk061-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk061-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk061.ucc6a7a72ce
n50_n50_rpu = 288

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BARCODE 61.0 = GACTAACCACATTAGC-1

using 6891 reads

====================================================================================

graph has 4532 edges initially, 50 edges after simplification

total ucounts = 821
nonsolo ucounts = 412[2^149, 3^77, 4^66, 5^32, 6^23, 7^12, 8^7, 9^5, 10, 11^3, 12^2, 13^2, 14^2, 19, 20, 23, 33, 45, 46, 56, 59, 77^2, 79, 96, 103, 132, 155, 168, 180, 184, 198, 206, 210, 214, 228, 259, 273, 274, 307, 314, 344, 350, 356]
surviving nonsolo ucounts = 27[20, 23, 33, 45, 46, 56, 59, 77, 79, 96, 132, 155, 168, 184, 198, 206, 210, 214, 228, 259, 273, 274, 307, 314, 344, 350, 356]
ids = [187, 689, 771, 575, 461, 145, 367, 254, 253, 238, ...]

====================================================================================

UMI info for barcode GACTAACCACATTAGC-1 contig 1 = GATCAGGACT...
umi ATCGACCATC = 345 reads: +400 validated
umi CTACAATCAT = 268 reads: +400 validated
umi TGCCGCTACC = 24 reads: +25 -16 +3 -1 +8 -1 +346 non-validated
umi TTGGTCTCGT = 307 reads: +400 validated

UMI info for barcode GACTAACCACATTAGC-1 contig 2 = GCTGTGCTGT...
umi ATTACTCCTC = 21 reads: +8 -7 +290 -49 +28 non-validated
umi CAGATATCGT = 215 reads: +382 validated
umi GCGCCGCATC = 45 reads: +382 validated
umi GGCAGTGGAG = 214 reads: +382 validated
umi TTCTACCCCC = 266 reads: +382 validated

UMI info for barcode GACTAACCACATTAGC-1 contig 3 = GGGGAGCTCT...
umi AATCATTACT = 217 reads: +427 validated
umi ATAATCTTCG = 49 reads: +292 -10 +101 -24 non-validated
umi ATCGTCTAGC = 238 reads: +427 validated
umi CACTATTCTA = 176 reads: +427 validated
umi CACTCGCCAC = 96 reads: +397 -1 +6 -2X +6 -1X +1 -1X +11 -1X invalidated
umi CATCAAGTGC = 77 reads: +417 -10 non-validated
umi CATCACCCCG = 74 reads: +424 -3 non-validated
umi CTACAGTACT = 167 reads: +403 -24 non-validated
umi CTACTATCCA = 60 reads: +400 -1 +26 non-validated
umi GGAATTCCAG = 362 reads: +427 validated
umi GTACGTCGCT = 130 reads: +427 validated
umi GTTCCGTAGC = 158 reads: +417 -1 +7 -2 non-validated
umi TAACGCCAGC = 314 reads: +427 validated
umi TAGCCACAGG = 45 reads: +391 -1 +6 -29 non-validated
umi TAGCCGACAC = 213 reads: +218 -1XX +208 invalidated
umi TCCCTCCTAA = 193 reads: +427 validated
umi TTCTCAGGTA = 35 reads: +34 -3X +6 -1 +170 -1 +156 -1 +2 -9 +16 -1 +2 -1 +2 -1 +1 -1 +17 -1 +1 invalidated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 4 umis using 124 reads
cdr3 = CMQALQTPPWTF at 366, score = 9 + 8
umis assigned: [164, 361, 689, 787]
of which 4 are surviving nonsolos
reads assigned: 933
start codons at 30, 63, 99, 187, 349, 369, 472
confident = true

TIG 2[bases=636]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
387-425 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
425-636 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 4 umis using 95 reads
cdr3 = CQSADSSGTYVVF at 358, score = 8 + 8
umis assigned: [187, 243, 461, 478, 767]
of which 5 are surviving nonsolos
reads assigned: 749
start codons at 43, 104, 173, 191, 386
confident = true

TIG 3[bases=581]
83-436 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=2)
443-464 ==> 0-21 on |32|IGHD6-13|D-REGION| [len=21] (mis=2)
462-510 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=0)
510-581 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 9 umis using 142 reads
cdr3 = CARDGVLYSSSWYYFDYW at 425, score = 9 + 7
umis assigned: [44, 145, 166, 237, 238, 253, 254, 362, 367, 472] and 7 others
of which 17 are surviving nonsolos
reads assigned: 2547
start codons at 83, 234, 239, 297, 300, 386, 435
confident = true
now this is a cell
NOT paired!

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BARCODE 61.3 = GACTAACCACCCATGG-1

using 3081 reads

====================================================================================

graph has 2361 edges initially, 38 edges after simplification

total ucounts = 526
nonsolo ucounts = 244[2^87, 3^62, 4^25, 5^15, 6^11, 7^10, 8^8, 9^4, 10^2, 11, 12^5, 13^2, 14, 16, 17, 57, 130, 154, 169, 182, 191, 296, 316, 369]
surviving nonsolo ucounts = 10[14, 57, 130, 154, 169, 182, 191, 296, 316, 369]
ids = [523, 274, 321, 92, 11, 59, 468, 138, 199, 90]

====================================================================================

UMI info for barcode GACTAACCACCCATGG-1 contig 1 = GGGGAGGAGT...
umi ACGTTTATCC = 183 reads: +385 validated
umi AGTCATGAGG = 374 reads: +385 validated
umi CACGTAACCC = 297 reads: +385 validated
umi CCTCTACTTT = 315 reads: +385 validated
umi TGTAAGGCTG = 189 reads: +385 validated

UMI info for barcode GACTAACCACCCATGG-1 contig 2 = GAGCTCTGGG...
umi AGTCTCCAAG = 153 reads: +418 validated
umi GAATTGCGGG = 58 reads: +418 validated
umi TTTGGTCAAC = 12 reads: +100 -1X +113 -4 +1 -1 +1 -1 +59 -36 +56 -45 invalidated

UMI info for barcode GACTAACCACCCATGG-1 contig 3 = AGTGCTTTCT...
umi AACTCAAATG = 162 reads: +436 validated
umi GGACAGCCTA = 13 reads: +5 -1XX +3 -2XX +1 -2XX +6 -1X +5 -1X +10 -50 +2 -2X +1 -2X +3 -1X +4 -1XX +1 -2XX +1 -1XX +2 -2XX +5 -1XX +26 -1XX +2 -1XX +9 -3XX +1 -2XX +2 -1XX +1 -1XX +3 -3XX +1 -1XX +2 -258X invalidated

GOOD CONTIGS

TIG 1[bases=552]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=23)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=7)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 208 reads
cdr3 = CQQTNNLPTF at 358, score = 9 + 7
umis assigned: [59, 90, 138, 199, 468]
of which 5 are surviving nonsolos
reads assigned: 1335
start codons at 31, 37, 93, 106, 245, 458
confident = true

TIG 2[bases=573]
0-80 ==> 0-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=3)
80-394 ==> 0-314 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=22)
450-498 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
498-573 ==> 0-75 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 2 umis using 16 reads
cdr3 = CARDRGTSRWYLDHW at 422, score = 7 + 5
umis assigned: [92, 274, 523]
of which 3 are surviving nonsolos
reads assigned: 219
start codons at 80, 167, 231, 294, 297, 315, 383, 448
confident = true

TIG 3[bases=528]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=41)
405-453 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
453-528 ==> 0-75 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 24 reads
cdr3 = CARYFAFVNYYFDKW at 377, score = 9 + 7
umis assigned: [11, 321]
of which 2 are surviving nonsolos
reads assigned: 174
start codons at 17, 38, 82
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.5 = GACTAACCACCGAAAG-1

using 293 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[287]
surviving nonsolo ucounts = 1[287]
ids = [5]

====================================================================================

UMI info for barcode GACTAACCACCGAAAG-1 contig 1 = GAGAAGAGCT...
umi TTCGTGTACA = 289 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=559]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
385-423 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQYGSSPLFTF at 359, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 285
start codons at 35, 243, 369, 465
confident = false
NOT paired!

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BARCODE 61.8 = GACTAACCACGAAAGC-1

using 33 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 61.9 = GACTAACCACGACTCG-1

using 52690 reads

====================================================================================

graph has 25188 edges initially, 168 edges after simplification

total ucounts = 3542
nonsolo ucounts = 1661[2^614, 3^339, 4^208, 5^118, 6^67, 7^42, 8^28, 9^13, 10^5, 11^11, 12^7, 13^5, 14^4, 15, 16^3, 18^3, 20^2, 21^2, 22, 23, 31, 33^2, 42, 44, 51, 53, 58, 61, 70, 75, 77, 78, 79, 85, 86, 91, 104, 113, 117, 119, 120, 123^2, 125^2, 126, 127, 133, 135, 137, 139, 146^3, 149, 153^2, 155, 158^3, 161, 165, 166, 170^2, 171, 173^2, 175, 179^2, 182, 185^2, 187, 189, 195, 197, 200^2, 201, 203, 205, 207, 209, 210, 212^2, 215, 219, 220, 221^2, 222^2, 224, 226, 227^2, 229, 233, 238^2, 239, 241, 244, 245, 251, 252^2, 253^2, 254, 255, 259^2, 260, 261^2, 264, 266, 269, 271, 274^3, 275^2, 276, 278^2, 279, 281, 282, 284^2, 285, 287, 288^2, 290^2, 291^2, 292^2, 293^3, 294, 296, 297^2, 298, 299, 301, 302, 304, 307, 308^2, 310^3, 311^3, 313, 315^3, 320, 321, 322, 324, 327, 328, 330, 336^4, 337, 338^2, 340, 341, 343^2, 354, 364, 372, 374, 377, 383^3, 387, 402, 429, 436, 452, 502, 638, 945]
surviving nonsolo ucounts = 176[21, 61, 75, 77, 78, 79, 91, 104, 113, 117, 119, 120, 123^2, 125^2, 126, 127, 133, 135, 137, 139, 146^3, 149, 153^2, 155, 158^2, 161, 165, 166, 170^2, 171, 173^2, 175, 179^2, 182, 185^2, 187, 189, 195, 197, 200^2, 201, 203, 205, 207, 209, 210, 212^2, 215, 219, 220, 221^2, 222^2, 224, 226, 227^2, 229, 233, 238^2, 239, 241, 244, 245, 251, 252^2, 253^2, 254, 255, 259^2, 260, 261^2, 264, 266, 269, 271, 274^3, 275^2, 276, 278^2, 279, 281, 282, 284^2, 285, 287, 288^2, 290^2, 291^2, 292^2, 293^3, 294, 296, 297^2, 298, 299, 301, 302, 304, 307, 308^2, 310^3, 311^3, 313, 315^3, 320, 321, 322, 324, 327, 328, 330, 336^4, 337, 338^2, 340, 341, 343^2, 354, 364, 372, 374, 377, 383^3, 387, 402, 429, 436, 452, 502, 638, 945]
ids = [473, 3038, 2049, 1952, 2487, 2470, 2581, 3202, 2174, 2655, ...]

====================================================================================

UMI info for barcode GACTAACCACGACTCG-1 contig 1 = TGGGAGGAGT...
umi AAACTAACCT = 219 reads: +382 validated
umi AAACTTGGGA = 263 reads: +382 validated
umi AAAGGGGCGA = 164 reads: +382 validated
umi AACTAGTGTG = 315 reads: +328 -2XX +1 -3XX +1 -4XX +43 invalidated
umi AACTTCTTAA = 244 reads: +382 validated
umi AAGAATCCCG = 180 reads: +382 validated
umi AAGCACGGGT = 309 reads: +382 validated
umi AAGTCGCGGC = 336 reads: +382 validated
umi AATAACTCCC = 294 reads: +119 -1XX +262 invalidated
umi AATCCTTGTG = 184 reads: +382 validated
umi ACCCAAGTCC = 236 reads: +382 validated
umi ACGATTGGTC = 296 reads: +382 validated
umi ACTCTGCTGG = 225 reads: +382 validated
umi AGAACATGTG = 138 reads: +382 validated
umi AGACGTCAAG = 324 reads: +382 validated
umi AGCCTCGGCC = 249 reads: +382 validated
umi AGCTGTTTCG = 261 reads: +382 validated
umi AGGATCTGCA = 332 reads: +382 validated
umi AGGTATTTTG = 270 reads: +382 validated
umi ATAAAGCCGT = 270 reads: +382 validated
umi ATAATGAACT = 292 reads: +382 validated
umi ATACATGGGG = 143 reads: +382 validated
umi ATAGAAGCAC = 340 reads: +382 validated
umi ATATCTGGGA = 329 reads: +382 validated
umi ATCAACATTT = 290 reads: +382 validated
umi ATCCATGACC = 314 reads: +382 validated
umi ATCCTCTGAA = 293 reads: +382 validated
umi ATCGAACGAC = 297 reads: +382 validated
umi ATGGCAACCT = 250 reads: +382 validated
umi ATGTTCGGGG = 360 reads: +382 validated
umi ATTGGGTACC = 270 reads: +382 validated
umi ATTGTAACGT = 300 reads: +382 validated
umi ATTGTCCTAT = 259 reads: +382 validated
umi CAATTCTATG = 378 reads: +382 validated
umi CACGTTTGCT = 274 reads: +382 validated
umi CACTAACCAC = 340 reads: +382 validated
umi CAGAGATTGG = 339 reads: +382 validated
umi CAGAGTATCG = 344 reads: +382 validated
umi CATATCCTCA = 404 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -53XX +1 -6XX +1 -2XX +1 -7XX +156 invalidated
umi CATCACACTT = 300 reads: +382 validated
umi CATGGGGTCC = 175 reads: +382 validated
umi CCACCATTTG = 315 reads: +382 validated
umi CCATATGATG = 259 reads: +382 validated
umi CCATGTTCAG = 325 reads: +382 validated
umi CCGAGTGACA = 462 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -2XX +1 -2X +1 -3X +1 -43X +1 -6XX +1 -2XX +1 -7XX +156 invalidated
umi CCGATTGCTC = 251 reads: +382 validated
umi CCTCACGCGG = 378 reads: +382 validated
umi CCTCGTCGTA = 239 reads: +382 validated
umi CCTCGTTCGC = 465 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -2XX +1 -50X +1 -6XX +1 -2XX +1 -7XX +156 invalidated
umi CGGACAGATA = 216 reads: +382 validated
umi CGGATTACAG = 128 reads: +382 validated
umi CGGCAGGGCA = 483 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -53XX +1 -6XX +1 -2XX +1 -7XX +156 invalidated
umi CGTCAGAGCT = 270 reads: +382 validated
umi CGTCGGTGTG = 179 reads: +283 -1XX +98 invalidated
umi CGTCTACCAC = 288 reads: +382 validated
umi CGTGGCTCAG = 331 reads: +382 validated
umi CGTGTTCTCA = 207 reads: +382 validated
umi CGTTGGGGGT = 282 reads: +382 validated
umi CTAGCTCGAT = 355 reads: +382 validated
umi CTAGTAGGGG = 271 reads: +143 -2XX +237 invalidated
umi CTATCGTATC = 279 reads: +382 validated
umi CTCCGGTCAT = 266 reads: +382 validated
umi CTCGCAAGAC = 121 reads: +382 validated
umi CTGCGCACCG = 319 reads: +382 validated
umi CTGTAGCTTA = 386 reads: +382 validated
umi CTTGGCAGTC = 290 reads: +382 validated
umi GAGGTTACAA = 120 reads: +382 validated
umi GATTCCCTTG = 278 reads: +382 validated
umi GCAATTCGAG = 232 reads: +382 validated
umi GCATAATGTC = 250 reads: +382 validated
umi GCCCTCGTGC = 328 reads: +382 validated
umi GCCTTCTCAT = 306 reads: +382 validated
umi GCTCTGTTGA = 115 reads: +382 validated
umi GGGCGTTCGT = 295 reads: +382 validated
umi GGGGATCCTC = 255 reads: +382 validated
umi GGTAATCCAC = 295 reads: +382 validated
umi GGTTCTATTT = 306 reads: +382 validated
umi GTCCATGTGA = 301 reads: +382 validated
umi GTCCGAAGAT = 267 reads: +382 validated
umi GTTACCTCGG = 322 reads: +382 validated
umi GTTCAAGGGC = 340 reads: +382 validated
umi GTTTGCTTAA = 385 reads: +382 validated
umi TAAACTGTCT = 429 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -2XX +1 -50XX +1 -6XX +1 -2XX +1 -7XX +156 invalidated
umi TAACGCGCCG = 93 reads: +382 validated
umi TAATTATCGT = 163 reads: +382 validated
umi TACATTCCGC = 274 reads: -127X +255 invalidated
umi TACTCCGTGC = 278 reads: +382 validated
umi TAGAATATTG = 297 reads: +382 validated
umi TAGATCATAC = 288 reads: +382 validated
umi TAGCCGAGGA = 291 reads: +382 validated
umi TATTGGGGTT = 242 reads: +382 validated
umi TCAAACCGTC = 293 reads: +382 validated
umi TCAATATCTT = 279 reads: +382 validated
umi TCACGAAGAT = 126 reads: +382 validated
umi TCATTTACGC = 307 reads: +382 validated
umi TCCATGTCGC = 291 reads: +382 validated
umi TCCATTATAA = 292 reads: +382 validated
umi TCTATGTCCT = 322 reads: +382 validated
umi TCTGAAGGGG = 284 reads: +382 validated
umi TCTTTGGGAC = 259 reads: +382 validated
umi TGACAAATCC = 338 reads: +382 validated
umi TGCACGTACA = 304 reads: +382 validated
umi TGCGGTACGT = 306 reads: +382 validated
umi TGCTATGCGA = 377 reads: +382 validated
umi TGGCGACTTT = 371 reads: +382 validated
umi TGGTATACTC = 640 reads: +382 validated
umi TGGTCTCTCC = 330 reads: +382 validated
umi TGGTTAGACG = 315 reads: +382 validated
umi TTACGTACGC = 342 reads: +213 -1XX +1 -3XX +164 invalidated
umi TTAGGCCCTC = 315 reads: +382 validated
umi TTATACGGAC = 251 reads: +382 validated
umi TTCATATTGT = 224 reads: +382 validated
umi TTGCCGAACG = 344 reads: +382 validated
umi TTGGTTAAGG = 310 reads: +382 validated
umi TTTTGACTAC = 312 reads: +382 validated
umi TTTTTGGGGT = 290 reads: +382 validated

UMI info for barcode GACTAACCACGACTCG-1 contig 2 = AGCCCTCAGA...
umi AAACACAGGA = 206 reads: +430 validated
umi AACAGCTCCC = 189 reads: +378 -1X +36 -15 invalidated
umi AACCTTTGGT = 208 reads: +430 validated
umi AATGGGCGCT = 242 reads: +421 -9 non-validated
umi ACGACAAGCG = 223 reads: +430 validated
umi ACTGTTCCGG = 184 reads: +412 -18 non-validated
umi AGATAGACTT = 21 reads: +13 -4 +88 -1 +119 -1X +8 -15 +1 -1 +11 -1 +102 -1 +35 -1 +13 -1 +5 -9 invalidated
umi AGCTTTGTTC = 202 reads: +430 validated
umi ATACAGGTGG = 122 reads: +420 -2 +8 non-validated
umi ATTGTTCCTT = 143 reads: +392 -38 non-validated
umi CAATATCTCG = 155 reads: +430 validated
umi CAATTGCATT = 188 reads: +430 validated
umi CACCACTCTC = 208 reads: +430 validated
umi CAGCGGAATC = 128 reads: +430 validated
umi CCCCTCTCTT = 487 reads: +1 -1XX +6 -1XX +6 -1XX +2 -8XX +1 -376X +1 -1XX +1 -3XX +1 -7XX +1 -3XX +1 -3XX +2 -1XX +2 invalidated
umi CCCTGGGCAC = 256 reads: +105 -1 +324 non-validated
umi CCGCTTCTGC = 160 reads: +430 validated
umi CGCTTCACCG = 147 reads: -9 +1 -1XX +1 -9XX +1 -3XX +1 -3XX +2 -1XX +1 -1XX +2 -1XX +1 -1XX +2 -1XX +1 -1XX +386 invalidated
umi CTAAGATCCA = 235 reads: +401 -1 +28 non-validated
umi CTAGGCCTCC = 177 reads: +393 -37 non-validated
umi CTCTATCGGT = 215 reads: +430 validated
umi CTGAATGGAA = 159 reads: +406 -24 non-validated
umi CTGACTTCTA = 199 reads: +412 -1 +17 non-validated
umi CTGAGCTCTT = 227 reads: +430 validated
umi CTGCACCCAG = 155 reads: +430 validated
umi CTTCATCGGG = 233 reads: +430 validated
umi GACAGAACCT = 289 reads: +430 validated
umi GACTCGTCTT = 79 reads: +377 -8 +45 non-validated
umi GAGGCTCCTT = 170 reads: +430 validated
umi GAGGTTTTAG = 218 reads: +430 validated
umi GATACGCTCG = 150 reads: +430 validated
umi GCAATTGGAC = 77 reads: +380 -1 +6 -43 non-validated
umi GCCTATTGAG = 206 reads: +430 validated
umi GCTTTGGGTT = 103 reads: +26 -3XX +2 -1XX +1 -1XX +2 -1XX +1 -1XX +2 -1XX +1 -1XX +363 -23 invalidated
umi GGCCTACGCC = 206 reads: +430 validated
umi GGCCTGCTTC = 158 reads: +430 validated
umi GGGCTTATGT = 211 reads: +398 -1 +11 -1 +1 -1 +17 non-validated
umi GGGGCGACGG = 123 reads: +430 validated
umi GGTTATCCCT = 280 reads: +430 validated
umi GTAAAATACG = 153 reads: +413 -1 +16 non-validated
umi GTAATACTCC = 200 reads: +430 validated
umi GTGAATGCCC = 240 reads: +195 -1XX +2 -1 +231 invalidated
umi GTGCCCCGAA = 80 reads: +426 -3 +1 non-validated
umi GTGGGTATAA = 76 reads: +363 -1 +66 non-validated
umi GTTAGCCGTA = 223 reads: +430 validated
umi TAACCACCGC = 124 reads: +426 -4 non-validated
umi TACCAGATCC = 112 reads: +419 -1 +10 non-validated
umi TACGCTTCGG = 141 reads: +212 -3X +215 invalidated
umi TAGAGACTTT = 207 reads: +430 validated
umi TCATGGTCTA = 928 reads: +1 -1XX +6 -1XX +6 -1XX +2 -8XX +1 -376X +1 -1XX +1 -3XX +1 -7XX +1 -3XX +1 -3XX +2 -1XX +2 invalidated
umi TCTCTAGCGC = 139 reads: +430 validated
umi TGACCCCTGG = 64 reads: +405 -1 +3 -1 +14 -2 +4 non-validated
umi TGATAGTTTA = 173 reads: +430 validated
umi TGCTGTACAC = 215 reads: +430 validated
umi TGGCCTACAT = 185 reads: +430 validated
umi TGGTGTATCT = 135 reads: +116 -1XX +313 invalidated
umi TGTGTTTCGG = 105 reads: +402 -1X +27 invalidated
umi TTCACCTCAG = 131 reads: +430 validated
umi TTCCTCTCCG = 171 reads: +419 -11 non-validated
umi TTTTCATGTG = 294 reads: -240 +190 non-validated

GOOD CONTIGS

TIG 1[bases=555]
37-285 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
381-419 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 115 umis using 5436 reads
cdr3 = CLHYDNRRRTF at 358, score = 9 + 8
umis assigned: [27, 29, 39, 108, 123, 131, 143, 165, 172, 192] and 106 others
of which 116 are surviving nonsolos
reads assigned: 32593
start codons at 37, 93, 106, 245, 368, 461
confident = true

TIG 2[bases=669]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=1)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=38)
463-509 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
509-669 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 42 umis using 587 reads
cdr3 = CARESYVGLYSSTSYPDYW at 421, score = 8 + 7
umis assigned: [19, 76, 94, 205, 343, 428, 473, 530, 643, 897] and 50 others
of which 59 are surviving nonsolos
reads assigned: 11118
start codons at 79, 228, 235, 314, 356, 382, 437, 563
confident = true
now this is a cell
paired!

GCTGTGTATTACTGTGCGCGAGAATCTTATGTCGGGCTCTATTCTTCAACCAGTTATCCCGACTACTGGGGTCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GTCTCCAGCCTGCAACCTGAAGATTTTGCCACCTATTATTGTCTACACTATGATAATCGCCGTCGCACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.11 = GACTAACCACGGTTTA-1

using 386 reads

====================================================================================

graph has 192 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 4, 374]
surviving nonsolo ucounts = 1[374]
ids = [4]

====================================================================================

UMI info for barcode GACTAACCACGGTTTA-1 contig 1 = GAGGAACTGC...
umi TACTGCGCCT = 378 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=6)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CQQRSNWPLTF at 354, score = 9 + 9
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 370
start codons at 33, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.14 = GACTAACCAGACAAGC-1

using 21 reads

====================================================================================

graph has 34 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^3, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.17 = GACTAACCAGATGAGC-1

using 365 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[365]
surviving nonsolo ucounts = 1[365]
ids = [0]

====================================================================================

UMI info for barcode GACTAACCAGATGAGC-1 contig 1 = GAGCTACAAC...
umi TAGAGCGTTG = 345 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=480]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=1)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=20)
391-430 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
430-480 ==> 0-50 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQQYYGSPRTF at 369, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 338
start codons at 27, 30, 85, 99, 352, 382, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.21 = GACTAACCAGCGTCCA-1

using 229 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 226]
surviving nonsolo ucounts = 1[226]
ids = [2]

====================================================================================

UMI info for barcode GACTAACCAGCGTCCA-1 contig 1 = AGGAGTCAGT...
umi TGGGAACCCA = 230 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 20-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQSYRRPITF at 354, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 27, 33, 89, 102, 238, 337, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.43 = GACTAACGTAATCACC-1

using 315 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 308]
surviving nonsolo ucounts = 1[308]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=524]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=1)
393-431 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
431-524 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 291
start codons at 30, 99, 352, 473
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.48 = GACTAACGTAGCTCCG-1

using 396 reads

====================================================================================

graph has 668 edges initially, 2 edges after simplification

total ucounts = 215
nonsolo ucounts = 74[2^31, 3^20, 4^12, 5^3, 6^3, 7^2, 9, 11, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.56 = GACTAACGTCATTAGC-1

using 209 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[13, 192]
surviving nonsolo ucounts = 1[192]
ids = [5]

====================================================================================

UMI info for barcode GACTAACGTCATTAGC-1 contig 1 = ACTTTCTGAG...
umi TCTTCGCCGT = 185 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=573]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=17)
400-450 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
450-573 ==> 0-123 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 31 reads
cdr3 = CARDFPGNYFTFDIW at 374, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 181
start codons at 35, 79, 159, 229, 431
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.57 = GACTAACGTCCAGTAT-1

using 42 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[37]
surviving nonsolo ucounts = 1[37]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=420]
0-321 ==> 30-351 on |225|IGKV1-27|L-REGION+V-REGION| [len=351] (mis=1)
319-358 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
358-420 ==> 0-62 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQKYNSAPYTF at 297, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 35
start codons at 32, 45, 181, 280, 400
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.58 = GACTAACGTCCGCTGA-1

using 34 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 29]
surviving nonsolo ucounts = 1[29]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.62 = GACTAACGTCTAAACC-1

using 2246 reads

====================================================================================

graph has 538 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 5[3^2, 9, 20, 2208]
surviving nonsolo ucounts = 1[2208]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.63 = GACTAACGTCTAACGT-1

using 250 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 8[2^3, 3^2, 4, 9, 217]
surviving nonsolo ucounts = 1[217]
ids = [4]

====================================================================================

UMI info for barcode GACTAACGTCTAACGT-1 contig 1 = AGGAGGCAGC...
umi CTTACCGTGT = 213 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=533]
30-391 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
380-418 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
418-533 ==> 0-115 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CSSYTSSSTLVF at 354, score = 8 + 9
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 30, 187, 231, 238, 241
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.68 = GACTAACGTGTTCGAT-1

using 508 reads

====================================================================================

graph has 804 edges initially, 2 edges after simplification

total ucounts = 251
nonsolo ucounts = 97[2^45, 3^22, 4^8, 5^4, 6^5, 7^5, 8, 9^2, 10, 11^3, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.69 = GACTAACGTTACGGAG-1

using 262 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[257]
surviving nonsolo ucounts = 1[257]
ids = [4]

====================================================================================

UMI info for barcode GACTAACGTTACGGAG-1 contig 1 = GTCAGACTCA...
umi GTTCTCCCGG = 255 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-363 ==> 0-340 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=14)
372-411 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQYSTFLFTF at 350, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 255
start codons at 23, 29, 85, 98, 234, 237, 330, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.79 = GACTAACTCAACACCA-1

using 478 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[473]
surviving nonsolo ucounts = 1[473]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=486]
5-39 ==> 5966-6000 on rc of segment after IGHV4-4 exon 1 [len=6000] (mis=0)
18-285 ==> 0-267 on |184|IGHV4-34|L-REGION+V-REGION| [len=369] (mis=26)
304-486 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
cdr3 = CLVKDYFPEPVTVSWNSGALTS at 381, score = 5 + 5
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 468
start codons at 18, 39, 83
confident = false
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.83 = GACTAACTCAGAGCTT-1

using 381 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 376]
surviving nonsolo ucounts = 1[376]
ids = [0]

====================================================================================

UMI info for barcode GACTAACTCAGAGCTT-1 contig 1 = GAGCTGCTCA...
umi ATGCCGACGC = 354 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=513]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=26)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
418-513 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYGSSPMYTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 350
start codons at 30, 79, 238, 337, 378, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 61.90 = GACTAACTCCATGAAC-1

using 509 reads

====================================================================================

graph has 228 edges initially, 18 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 10, 70, 424]
surviving nonsolo ucounts = 2[70, 424]
ids = [2, 0]

====================================================================================

UMI info for barcode GACTAACTCCATGAAC-1 contig 1 = GGGGAGGAAC...
umi ACCCCACCCT = 428 reads: +6 -1XX +7 -1XX +34 -2XX +2 -4XX +1 -2XX +1 -1XX +1 -7XX +1 -281X +1 -3X +1 -13X +1 -3XX +1 -1XX +2 -2XX +1 -1XX invalidated
umi ATATTACAGC = 72 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 8 reads
cdr3 = CQQRSNWPLTF at 357, score = 9 + 9
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 484
start codons at 36, 244, 460
confident = false
NOT paired!

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BARCODE 61.91 = GACTAACTCCATGAGT-1

using 190 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 186]
surviving nonsolo ucounts = 1[186]
ids = [0]

====================================================================================
NOT paired!

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BARCODE 61.96 = GACTAACTCCGCGTTT-1

using 56 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 12[2^5, 4^4, 5, 6, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 61.97 = GACTAACTCCGTCAAA-1

using 710 reads

====================================================================================

graph has 1034 edges initially, 4 edges after simplification

total ucounts = 314
nonsolo ucounts = 153[2^65, 3^36, 4^17, 5^9, 6^12, 7^3, 8^6, 9, 10^2, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 61.115 = GACTAACTCTACTATC-1

using 279 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 275]
surviving nonsolo ucounts = 1[275]
ids = [1]

====================================================================================

UMI info for barcode GACTAACTCTACTATC-1 contig 1 = ACCCAAAAAC...
umi CACCTCTTAT = 272 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=555]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-555 ==> 0-65 on |1|IGHA1|C-REGION| [len=1058] (mis=1)
junction support: 1 umis using 24 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 54, 252, 257, 274, 318, 351, 544
confident = false
NOT paired!

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BARCODE 61.125 = GACTAACTCTTGCCGT-1

using 1149 reads

====================================================================================

graph has 1668 edges initially, 10 edges after simplification

total ucounts = 562
nonsolo ucounts = 221[2^86, 3^65, 4^25, 5^14, 6^11, 7^3, 8^4, 9^2, 10^2, 11^2, 12^2, 13^2, 14^3]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 61.130 = GACTACAAGACACGAC-1

using 241 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[8, 231]
surviving nonsolo ucounts = 1[231]
ids = [0]

====================================================================================

UMI info for barcode GACTACAAGACACGAC-1 contig 1 = AGTCTCAGTC...
umi AAGAATCCTG = 204 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=436]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=28)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
411-436 ==> 0-25 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQSHNTPPWTF at 347, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 201
start codons at 20, 26, 82, 95, 231, 234
confident = false
NOT paired!

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BARCODE 61.132 = GACTACAAGACGACGT-1

using 476 reads

====================================================================================

graph has 680 edges initially, 6 edges after simplification

total ucounts = 213
nonsolo ucounts = 85[2^24, 3^20, 4^9, 5^11, 6^10, 7^3, 8^5, 9^2, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 61.134 = GACTACAAGACTGTAA-1

using 152 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 4, 143]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.09
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk061-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk061-ucc6a7a72ce/files/contig_bam.bam

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15.434 seconds used processing barcodes, peak mem = 0.27
