[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.24 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GE...
n50_n50_rpu = 288

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BARCODE 45.0 = CTACATTCAGCGAACA-1

using 936 reads

====================================================================================

graph has 329 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 288, 642]
surviving nonsolo ucounts = 2[288, 642]
ids = [0, 4]

====================================================================================

UMI info for barcode CTACATTCAGCGAACA-1 contig 1 = AGTCTCAGTC...
umi GCCACGTAAC = 635 reads: +388 validated

UMI info for barcode CTACATTCAGCGAACA-1 contig 2 = GCTGCGGGTA...
umi ACAAAACGGG = 283 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 27-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
20-371 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
370-408 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 104 reads
cdr3 = CQQSYRRPITF at 347, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 626
start codons at 20, 26, 82, 95, 231, 330, 450
confident = false

TIG 2[bases=523]
0-40 ==> 0-40 on |318|IGLV1-36|5'UTR| [len=40] (mis=0)
40-393 ==> 0-353 on |319|IGLV1-36|L-REGION+V-REGION| [len=353] (mis=3)
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
428-523 ==> 0-95 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CAAWDDSLSGPVF at 361, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 40, 191, 248, 251, 344, 369, 374
confident = false
NOT paired!

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BARCODE 45.8 = CTACATTCAGTCACTA-1

using 80000 reads

====================================================================================

graph has 14800 edges initially, 26 edges after simplification

total ucounts = 7496
nonsolo ucounts = 6938[2^267, 3^263, 4^291, 5^394, 6^432, 7^487, 8^576, 9^601, 10^531, 11^559, 12^496, 13^382, 14^316, 15^261, 16^170, 17^146, 18^95, 19^94,...
surviving nonsolo ucounts = 6468[2^61, 3^123, 4^238, 5^380, 6^420, 7^482, 8^572, 9^597, 10^530, 11^555, 12^494, 13^381, 14^311, 15^258, 16^169, 17^145, 18^8...
ids = [68, 80, 125, 253, 707, 736, 1078, 1089, 1328, 1428, ...]

====================================================================================

UMI info for barcode CTACATTCAGTCACTA-1 contig 1 = GAGCTCTGGG...
umi AAAAACGGCC = 6 reads: -38 +186 -1 +84 -121 non-validated
umi AAAACACTGG = 6 reads: +6 -1 +31 -24 +108 -27 +56 -177 non-validated
umi AAAACCATTA = 5 reads: -11 +1 -1 +4 -1 +2 -1 +6 -1 +33 -1 +2 -1 +2 -36 +2 -1 +21 -1 +165 -137 non-validated
umi AAAAGAGCCC = 14 reads: +2 -1 +221 -1 +16 -1 +82 -106 non-validated
umi AAAAGCAGGT = 10 reads: +40 -1 +10 -1 +56 -6 +5 -1 +7 -1 +4 -2 +1 -1 +2 -1 +6 -1 +123 -1 +10 -150 non-validated
umi AAAAGGGCTA = 11 reads: +91 -1 +196 -9 +79 -1 +24 -29 non-validated
umi AAAATATCAA = 11 reads: -315 +100 -15 non-validated
umi AAACAGTTGT = 8 reads: -7 +76 -86 +2 -1 +5 -1 +24 -1X +79 -148 invalidated
umi AAACATTCAA = 10 reads: -21 +77 -15 +1 -1 +3 -2 +8 -1 +5 -1 +8 -1 +71 -1 +58 -1 +1 -1 +2 -3 +10 -1 +3 -1 +41 -92 non-validated
umi AAACGACACC = 10 reads: +94 -1 +129 -61 +1 -1 +6 -1 +2 -2 +1 -1 +5 -1 +3 -1 +4 -3 +5 -4 +13 -91 non-validated
umi AAACGATCAA = 10 reads: +73 -1 +5 -1 +24 -1X +71 -1 +3 -1 +3 -1 +8 -1 +1 -1 +3 -9 +56 -166 invalidated
umi AAACGGCTGC = 12 reads: +191 -68 +9 -1 +47 -1 +1 -1 +10 -101 non-validated
umi AAACGGGGTC = 6 reads: +44 -29 +56 -36 +99 -90 +9 -1 +5 -1 +2 -1 +3 -1 +2 -1 +50 non-validated
umi AAACTCGGAC = 12 reads: +174 -18 +109 -129 non-validated
umi AAACTGAGGG = 2 reads: -69 +1 -1 +12 -1 +21 -1 +5 -1 +64 -254 non-validated
umi AAAGATACGC = 3 reads: -45 +46 -1 +9 -68 +56 -103 +56 -46 non-validated
umi AAAGATCAGC = 2 reads: -142 +56 -23 +1 -1 +5 -1 +13 -1 +1 -1 +4 -2 +5 -2 +11 -1 +5 -1 +1 -153 non-validated
umi AAAGGATACA = 11 reads: -11 +76 -1 +44 -13 +179 -13 +40 -1 +15 -37 non-validated
umi AAAGGCCCTC = 7 reads: -117 +1 -1 +118 -64 +1 -1 +1 -2 +1 -4X +1 -1X +1 -1 +1 -4X +1 -1 +1 -2 +1 -1 +8 -3 +2 -2 +10 -1 +2 -32 +43 invalidated
umi AAAGGTCGGC = 3 reads: -99 +6 -1 +14 -1 +3 -1 +13 -1 +93 -198 non-validated
umi AAAGTACGGT = 10 reads: +32 -1 +18 -100 +63 -14 +10 -1 +6 -1 +8 -1 +1 -1 +3 -1 +1 -1 +7 -1 +1 -1X +1 -1 +2 -1 +5 -8 +25 -1X +31 -22 +2 -1 +1 -1 +2 -1 +1 ...
umi AAAGTCATTC = 3 reads: -111 +56 -11 +10 -1 +15 -1 +52 -1 +5 -167 non-validated
umi AAATAAATGC = 5 reads: -140 +40 -1 +4 -1 +11 -1 +102 -38 +6 -3 +5 -1 +6 -1 +3 -1 +9 -1 +1 -1 +3 -1 +2 -1 +2 -1 +7 -37 non-validated
... 29929 lines elided ...
umi TTTCATAGGA = 3 reads: -124 +56 -3 +56 -35 +56 -64 non-validated
umi TTTCATAGTA = 6 reads: -52 +169 -165 +8 non-validated
umi TTTCATATCG = 10 reads: -28 +171 -116 +56 -23 non-validated
umi TTTCATATGG = 4 reads: -65 +56 -22 +84 -23 +56 -88 non-validated
umi TTTCATCTCG = 3 reads: -66 +56 -101 +56 -72 +37 -1 +5 non-validated
umi TTTCATGCGG = 5 reads: -16 +56 -81 +70 -80 +24 -1 +7 -1 +9 -1X +8 -2 +3 -35 invalidated
umi TTTCATGGTA = 5 reads: +3 -232 +56 -103 non-validated
umi TTTCATTACA = 9 reads: +3 -1X +35 -38 +56 -43 +137 -81 invalidated
umi TTTCATTGTT = 3 reads: -13 +56 -10 +65 -1 +4 -245 non-validated
umi TTTCCAATGA = 3 reads: -141 +56 -49 +98 -50 non-validated
umi TTTCCACACA = 7 reads: -31 +2 -1 +8 -1 +5 -1 +32 -1 +5 -11 +56 -28 +58 -94 +7 -1 +1 -1 +50 non-validated
umi TTTCCACCTC = 3 reads: -90 +3 -1 +76 -68 +3 -1 +2 -1 +6 -1 +4 -3 +10 -2 +2 -1 +16 -2 +2 -100 non-validated
umi TTTCCATAGC = 4 reads: -77 +2 -2 +2 -1 +3 -2X +2 -1 +7 -1 +2 -2 +5 -1 +4 -1 +7 -1 +5 -1 +2 -1 +1 -13 +2 -1 +63 -1 +1 -180 invalidated
umi TTTCCCAATA = 6 reads: +49 -1 +9 -1 +1 -1 +22 -20 +86 -204 non-validated
umi TTTCCCGACG = 4 reads: +33 -6 +81 -167 +56 -51 non-validated
umi TTTCCCGATA = 4 reads: -24 +56 -17 +2 -1 +1 -3 +1 -1 +1 -2 +1 -1 +6 -1 +5 -1 +69 -73 +5 -1 +2 -2 +2 -1 +3 -3 +2 -1 +3 -1 +4 -3 +2 -4 +1 -1 +5 -1 +3 -1 +4...
umi TTTCCGCATC = 9 reads: -49 +56 -5 +110 -2 +4 -32 +56 -20 +56 -4 non-validated
umi TTTCCGTAAT = 18 reads: -8 +56 -301X +1 -1X +1 -1X +1 -4X +1 -1X +1 -4XX +13 invalidated
umi TTTCCTAGGA = 3 reads: +35 -161 +5 -1 +7 -1 +1 -2 +39 -46 +9 -1 +13 -1 +30 -1 +1 -40 non-validated
umi TTTCCTAGTA = 9 reads: +5 -1 +8 -1 +33 -1 +117 -53 +73 -40 +9 -1 +7 -1 +10 -2 +13 -1 +12 -6 non-validated
umi TTTCCTCTGA = 4 reads: -149 +61 -1 +56 -47 +56 -24 non-validated
umi TTTCCTGGGT = 5 reads: -96 +56 -5 +75 -39 +83 -40 non-validated
umi TTTCGATCAG = 9 reads: -37 +11 -1 +177 -71 +56 -41 non-validated
umi TTTCGATCCG = 4 reads: -234 +5 -1 +7 -1 +3 -1 +9 -1 +58 -24 +50 non-validated
umi TTTCGCACGT = 5 reads: -115 +105 -12 +56 -69 +3 -1 +33 non-validated
umi TTTCGCCATG = 3 reads: -4 +29 -1 +10 -2 +14 -242 +1 -1 +1 -1 +2 -1 +1 -1 +6 -1 +5 -1 +1 -1 +1 -2 +2 -1 +1 -1 +3 -2 +3 -1 +2 -1 +2 -1 +5 -40 non-validated
umi TTTCGCGAAT = 7 reads: -11 +4 -1 +1 -1X +49 -37 +109 -29 +63 -1 +2 -1 +11 -1 +4 -2 +1 -2 +2 -1 +1 -60 invalidated
umi TTTCGCTTAA = 5 reads: -77 +56 -10 +101 -150 non-validated
umi TTTCGGCAAA = 11 reads: +42 -7 +56 -33 +86 -49 +102 -19 non-validated
umi TTTCGGCATG = 12 reads: -31 +57 -1 +29 -1 +2 -1 +1 -1 +9 -1 +1 -3 +94 -15 +65 -71 +11 non-validated
umi TTTCGGCGCT = 3 reads: -206 +29 -1 +26 -9 +56 -67 non-validated
umi TTTCGGGTCA = 6 reads: -101 +10 -2 +58 -15 +106 -10 +56 -36 non-validated
umi TTTCGTAATC = 2 reads: -89 +29 -1 +26 -83 +1 -1 +5 -1 +18 -1 +15 -1 +13 -110 non-validated
umi TTTCGTCAGG = 2 reads: -52 +56 -258 +28 non-validated
umi TTTCGTCCAC = 3 reads: -245 +59 -79 +11 non-validated
umi TTTCGTCTTG = 2 reads: -37 +59 -298 non-validated
umi TTTCGTGCAG = 5 reads: -12 +56 -56 +31 -1 +60 -1 +29 -85 +56 -7 non-validated
umi TTTCGTTCAT = 6 reads: -25 +151 -218 non-validated
umi TTTCGTTGGC = 6 reads: -47 +3 -1 +5 -2 +6 -1 +5 -1 +62 -24 +56 -29 +6 -1 +57 -88 non-validated
umi TTTCGTTTCT = 3 reads: -176 +1 -1 +1 -1 +1 -4 +7 -1 +73 -1X +1 -126 invalidated
umi TTTCTAAATG = 2 reads: -57 +56 -62 +1 -1 +54 -163 non-validated
umi TTTCTAACGG = 2 reads: -77 +56 -89 +20 -1 +35 -116 non-validated
umi TTTCTCACAT = 4 reads: -55 +56 -47 +56 -117 +18 -1 +37 -7 non-validated
umi TTTCTCCCAC = 3 reads: -71 +85 -83 +2 -1X +1 -1 +4 -1 +1 -1 +1 -1 +1 -1 +2 -1 +1 -1 +2 -3 +3 -2 +1 -1 +1 -1 +5 -1 +2 -1 +2 -2 +5 -1 +1 -100 invalidated
umi TTTCTCTCCT = 4 reads: -83 +22 -1 +33 -15 +6 -1 +50 -1 +2 -1 +51 -128 non-validated
umi TTTCTCTCGG = 7 reads: +11 -11 +60 -1 +4 -1 +3 -1 +13 -1 +21 -42 +1 -1 +69 -73 +2 -1 +14 -1 +4 -1 +20 -1 +13 -1 +2 -2 +3 -2 +5 -3 +4 -2 non-validated
umi TTTCTCTCTT = 4 reads: +23 -39 +1 -1 +11 -1 +4 -1 +3 -1 +9 -1 +7 -1 +5 -1 +9 -82 +56 -138 non-validated
umi TTTCTGGGCA = 2 reads: -336 +56 -2 non-validated
umi TTTCTGTTGT = 4 reads: -212 +56 -41 +38 -1 +3 -1 +1 -1 +2 -1 +37 non-validated
umi TTTCTTACGT = 3 reads: -96 +129 -169 non-validated
umi TTTCTTGCCG = 4 reads: -82 +11 -1 +73 -227 non-validated
umi TTTGAAAGGG = 4 reads: +31 -20 +3 -1 +1 -3 +2 -1 +8 -2 +1 -1 +1 -2 +5 -1 +1 -1 +2 -1 +4 -1 +4 -1 +7 -59 +56 -82 +55 -37 non-validated
umi TTTGAACCGT = 2 reads: -113 +56 -104 +17 -1 +38 -65 non-validated
umi TTTGAAGACC = 7 reads: +67 -1 +43 -91 +56 -17 +73 -46 non-validated
umi TTTGACGCTC = 3 reads: -153 +7 -1 +48 -111X +8 -2 +3 -2X +2 -1 +3 -2 +5 -1 +1 -2 +1 -1 +2 -2 +4 -1X +1 -1 +1 -1 +1 -1 +2 -2 +6 -1 +2 -1 +1 -1 +9 invalidated
umi TTTGACTCGC = 13 reads: +18 -1 +6 -1 +5 -1 +60 -24 +176 -102 non-validated
umi TTTGACTCTG = 9 reads: -35 +87 -3 +90 -1XX +16 -1 +60 -69 +2 -2X +2 -1 +4 -1 +1 -3 +6 -1 +4 -2 +1 -2 invalidated
umi TTTGAGACTG = 6 reads: -39 +106 -1 +20 -1 +4 -87 +86 -50 non-validated
umi TTTGAGGTCT = 3 reads: -52 +97 -87 +11 -1 +11 -1 +1 -1 +30 -102 non-validated
umi TTTGATAATC = 7 reads: +21 -105 +86 -1 +2 -1 +10 -1 +6 -1 +6 -1 +2 -42 +56 -53 non-validated
umi TTTGATCGGT = 3 reads: -108 +65 -1 +6 -1 +23 -27 +56 -107 non-validated
umi TTTGATGATT = 3 reads: -261 +70 -1 +6 -2 +18 -4 +32 non-validated
umi TTTGATTGCT = 5 reads: -116 +56 -4 +76 -142 non-validated
umi TTTGCATCTG = 3 reads: -19 +1 -1X +54 -17 +1 -1 +2 -1 +2 -1 +81 -213 invalidated
umi TTTGCGGGAA = 1 reads: -179 +56 -159 non-validated
umi TTTGCGGTCA = 5 reads: +46 -11 +107 -219 +11 non-validated
umi TTTGCGGTCT = 2 reads: -125 +56 -5 +28 -1 +27 -152 non-validated
umi TTTGCGTATA = 5 reads: -62 +72 -3 +56 -67 +1 -1 +11 -1 +2 -1 +4 -1 +1 -1 +7 -1 +3 -1 +11 -2 +5 -1 +1 -78 non-validated
umi TTTGCGTCGC = 3 reads: -31 +62 -248 +53 non-validated
umi TTTGCTACGC = 7 reads: -78 +1 -1 +10 -3X +1 -1 +1 -1 +2 -1 +60 -24 +9 -1 +25 -1 +12 -1 +7 -71 +24 -1 +5 -1 +52 invalidated
umi TTTGCTGCAT = 8 reads: -47 +56 -7 +90 -8 +76 -30 +80 non-validated
umi TTTGCTTACT = 2 reads: -36 +5 -1 +61 -291 non-validated
umi TTTGCTTGGT = 6 reads: -1 +27 -18 +56 -20 +65 -1 +5 -121 +56 -24 non-validated
umi TTTGGACCAA = 4 reads: -212 +2 -1 +92 -87 non-validated
umi TTTGGACCGT = 2 reads: -45 +56 -13 +56 -224 non-validated
umi TTTGGCCCCG = 3 reads: -10 +72 -1 +31 -59 +56 -165 non-validated
umi TTTGGCCTTG = 2 reads: -35 +56 -303 non-validated
umi TTTGGCGCAA = 7 reads: -82 +56 -3 +151 -29 +56 -17 non-validated
umi TTTGGCGGCT = 4 reads: -159 +17 -1 +96 -121 non-validated
umi TTTGGCTACC = 10 reads: +56 -41 +2 -1 +75 -8 +83 -51 +22 -1 +6 -1 +7 -1 +9 -1 +13 -1 +1 -1 +4 -1 +8 non-validated
umi TTTGGCTCTC = 5 reads: -54 +198 -142 non-validated
umi TTTGGTAAGG = 7 reads: -9 +56 -1 +10 -1 +2 -1 +3 -1 +9 -1 +99 -15 +56 -6 +56 -68 non-validated
umi TTTGGTGAGC = 5 reads: -15 +56 -112 +60 -1 +1 -149 non-validated
umi TTTGGTTCGG = 5 reads: -55 +6 -1 +2 -1 +7 -2 +6 -1 +24 -1 +5 -98 +55 -1 +2 -4 +71 -52 non-validated
umi TTTGGTTCTA = 11 reads: -36 +18 -1X +37 -2 +55 -8 +168 -1 +1 -67 invalidated
umi TTTGTACCAT = 3 reads: -394 non-validated
umi TTTGTACTAG = 3 reads: -168 +44 -1 +11 -17 +6 -1 +6 -1 +2 -1 +12 -1 +7 -1 +16 -1 +1 -97 non-validated
umi TTTGTACTTC = 8 reads: +19 -1 +1 -117 +114 -16 +56 -26 +44 non-validated
umi TTTGTAGGCT = 3 reads: -59 +56 -20 +56 -46 +56 -101 non-validated
umi TTTGTATCAC = 5 reads: -144 +16 -1 +81 -136 +16 non-validated
umi TTTGTCGTTG = 5 reads: -3 +1 -2 +1 -1X +6 -2 +4 -1 +5 -1 +56 -50 +56 -195 +10 invalidated
umi TTTGTCTCGC = 4 reads: -52 +56 -47 +6 -1 +19 -1 +3 -1 +6 -1 +16 -1 +56 -128 non-validated
umi TTTGTGATTA = 3 reads: -117 +56 -120 +60 -41 non-validated
umi TTTGTGCTGC = 2 reads: -37 +56 -89 +56 -156 non-validated
umi TTTGTTAACA = 1 reads: -186 +56 -152 non-validated
umi TTTGTTCCGA = 1 reads: -103 +56 -235 non-validated
umi TTTGTTCCTG = 4 reads: -25 +56 -31 +1 -1 +5 -1 +3 -1 +2 -1X +14 -1 +26 -6 +4 -1 +3 -1 +7 -2 +1 -1 +73 -127 invalidated
umi TTTGTTGGCT = 2 reads: -161 +56 -86 +56 -35 non-validated
umi TTTGTTGTCG = 5 reads: -27 +56 -53 +65 -20 +1 -2 +1 -1 +4 -1 +9 -1 +16 -1 +5 -1 +13 -53 +19 -1 +16 -1 +19 -8 non-validated
umi TTTGTTTTGG = 8 reads: +97 -36 +107 -62 +56 -14 +3 -1 +5 -1 +12 non-validated
umi TTTTAAATGC = 5 reads: -72 +15 -1 +5 -1 +34 -29 +19 -1 +36 -4 +3 -1 +31 -1 +20 -40 +17 -1 +38 -25 non-validated
umi TTTTAAGCTA = 2 reads: -124 +56 -111 +1 -1 +1 -6 +26 -1 +4 -1 +1 -1 +1 -1 +1 -2 +8 -47 non-validated
umi TTTTAATGAA = 7 reads: -2 +69 -63 +42 -1 +13 -46 +116 -42 non-validated
umi TTTTACGCAA = 4 reads: -77 +56 -118 +4 -2 +1 -1 +3 -2 +10 -2 +1 -1 +6 -1X +56 -1 +1 -51 invalidated
umi TTTTACGGTA = 2 reads: -100 +76 -1 +9 -208 non-validated
umi TTTTATAGTC = 4 reads: -116 +56 -85 +56 -9 +72 non-validated
umi TTTTATCCAT = 2 reads: +45 -46 +56 -247 non-validated
umi TTTTATGCCG = 2 reads: -108 +57 -229 non-validated
umi TTTTATGGGC = 1 reads: -57 +56 -281 non-validated
umi TTTTATTAGT = 11 reads: -15 +115 -16 +76 -1 +5 -10 +105 -37 +2 -2 +1 -2 +3 -1X +2 -1 invalidated
umi TTTTATTGAA = 7 reads: +39 -51 +171 -133 non-validated
umi TTTTATTTCG = 9 reads: +40 -43 +194 -61 +56 non-validated
umi TTTTCAGTTG = 4 reads: -104 +62 -40 +56 -9 +56 -67 non-validated
umi TTTTCATTGT = 9 reads: -100 +120 -18 +53 -1XX +5 -97 invalidated
umi TTTTCATTTA = 5 reads: -136 +4 -1 +51 -5 +120 -1 +12 -1 +1 -1 +6 -1 +1 -1 +14 -1 +6 -31 non-validated
umi TTTTCATTTC = 3 reads: +8 -197 +56 -82 +51 non-validated
umi TTTTCCACTC = 3 reads: +11 -1 +36 -1 +1 -127 +67 -150 non-validated
umi TTTTCCGAGC = 3 reads: -57 +9 -1 +5 -1 +9 -1 +2 -1 +7 -1 +1 -1 +16 -131 +106 -45 non-validated
umi TTTTCCTGAC = 2 reads: -34 +3 -1 +3 -4 +3 -1 +8 -1 +13 -1 +8 -1 +6 -2 +1 -151 +14 -1 +41 -97 non-validated
umi TTTTCGAATG = 8 reads: -51 +2 -1 +4 -1 +4 -1 +97 -3 +50 -1 +5 -38 +5 -1 +4 -1 +9 -1 +5 -1 +6 -1 +1 -1 +3 -2 +9 -1 +5 -80 non-validated
umi TTTTCGACCA = 1 reads: -135 +56 -203 non-validated
umi TTTTCGACTA = 8 reads: -29 +180 -185 non-validated
umi TTTTCGCCTT = 5 reads: -16 +85 -249 +1 -1 +42 non-validated
umi TTTTCGCTTG = 7 reads: +27 -49 +80 -124 +64 -30 +6 -1 +3 -2 +2 -4 +2 non-validated
umi TTTTCGTCAT = 5 reads: -133 +96 -33 +11 -1 +44 -76 non-validated
umi TTTTCGTGGC = 7 reads: +20 -1 +5 -45 +64 -8 +56 -32 +74 -89 non-validated
umi TTTTCTATCA = 5 reads: -1 +56 -10 +62 -43 +56 -166 non-validated
umi TTTTCTCAAG = 6 reads: -27 +84 -58 +17 -1 +102 -105 non-validated
umi TTTTCTTCTA = 3 reads: -56 +56 -188 +59 -35 non-validated
umi TTTTGAGACT = 3 reads: -108 +10 -2 +2 -1 +12 -1 +9 -1 +18 -38 +56 -136 non-validated
umi TTTTGAGTTA = 2 reads: -324 +70 non-validated
umi TTTTGATTGG = 4 reads: -149 +2 -1 +1 -2 +4 -1 +1 -1 +2 -1 +1 -1 +2 -2 +2 -1 +6 -1 +78 -26 +1 -1 +15 -1 +38 -53 non-validated
umi TTTTGCACTT = 1 reads: -24 +56 -314 non-validated
umi TTTTGCCCAA = 5 reads: -67 +56 -1 +56 -119 +95 non-validated
umi TTTTGCGGGT = 6 reads: -52 +115 -1 +5 -1 +4 -1 +1 -37 +8 -1 +59 -22 +19 -1 +5 -1 +4 -1 +3 -1 +5 -1 +4 -2 +2 -1 +6 -31 non-validated
umi TTTTGGGCTG = 6 reads: -33 +114 -41 +56 -150 non-validated
umi TTTTGGGTTT = 9 reads: -16 +56 -7 +138 -2 +56 -117 +2 non-validated
umi TTTTGGTAGG = 3 reads: -127 +13 -2 +81 -1 +24 -146 non-validated
umi TTTTGGTGCC = 4 reads: -121 +116 -1 +2 -154 non-validated
umi TTTTGGTGCG = 4 reads: -39 +8 -2 +140 -52 +56 -97 non-validated
umi TTTTGGTTTG = 5 reads: -106 +204 -84 non-validated
umi TTTTGTGCCA = 6 reads: -6 +56 -38 +82 -187 +25 non-validated
umi TTTTGTGTAT = 4 reads: -55 +29 -1X +73 -1 +25 -210 invalidated
umi TTTTTAAACG = 5 reads: -65 +124 -176 +12 -1 +10 -1 +5 non-validated
umi TTTTTAACCC = 6 reads: -50 +7 -1 +48 -19 +1 -3X +5 -1 +1 -1 +3 -2 +3 -1 +2 -1 +14 -1 +114 -95 +21 invalidated
umi TTTTTAAGCC = 9 reads: -57 +56 -16 +96 -1 +6 -1 +9 -1 +7 -1 +4 -1 +2 -2 +5 -58 +11 -1 +4 -1 +22 -1 +16 -15 non-validated
umi TTTTTAATAG = 3 reads: +26 -16 +49 -1 +6 -28 +56 -212 non-validated
umi TTTTTAATCA = 3 reads: -11 +56 -97 +19 -1 +36 -71 +56 -47 non-validated
umi TTTTTAATGG = 6 reads: +67 -22 +83 -13 +9 -2 +57 -141 non-validated
umi TTTTTAGCAC = 2 reads: -16 +5 -1 +1 -3 +3 -2 +7 -2 +1 -1 +1 -1 +4 -2 +5 -5 +4 -1 +4 -325X invalidated
umi TTTTTAGCAT = 5 reads: -15 +58 -1 +14 -1 +1 -128 +131 -45 non-validated
umi TTTTTATAGT = 2 reads: -10 +56 -287 +41 non-validated
umi TTTTTATTGC = 5 reads: -65 +56 -175 +5 -1 +29 -1 +62 non-validated
umi TTTTTCAGGC = 7 reads: -52 +133 -20 +76 -113 non-validated
umi TTTTTCCACC = 5 reads: -64 +56 -98 +10 -1 +82 -21 +5 -1 +2 -1 +2 -1 +2 -1 +2 -1 +1 -5 +6 -1 +5 -1 +1 -1 +1 -3X +3 -1 +1 -1 +1 -12 invalidated
umi TTTTTCGGCA = 5 reads: -75 +126 -20X +1 -1 +3 -1 +2 -1 +5 -1 +7 -1 +1 -1 +12 -1X +2 -1 +13 -119 invalidated
umi TTTTTCTACT = 7 reads: -68 +145 -9 +56 -47 +56 -13 non-validated
umi TTTTTCTCAT = 5 reads: -1 +72 -1X +90 -70 +56 -104 invalidated
umi TTTTTCTGGG = 5 reads: +8 -1 +15 -1 +1 -1 +6 -1 +3 -1 +112 -1 +6 -1 +42 -194 non-validated
umi TTTTTCTGTT = 5 reads: -28 +20 -1 +35 -20 +119 -69 +56 -46 non-validated
umi TTTTTCTTAA = 4 reads: -90 +95 -2 +10 -1 +64 -132 non-validated
umi TTTTTGAAAC = 7 reads: -35 +8 -1X +2 -1 +90 -220 +6 -1 +30 invalidated
umi TTTTTGCAAC = 6 reads: -100 +119 -175 non-validated
umi TTTTTGTATT = 5 reads: -72 +56 -42 +56 -45 +3 -1 +9 -2X +1 -2 +7 -1 +1 -1 +5 -1 +2 -1 +4 -1 +8 -1 +2 -1 +1 -68 invalidated
umi TTTTTTAACA = 4 reads: -13 +62 -1 +4 -1 +1 -2 +9 -1 +19 -1 +8 -62 +77 -133 non-validated
umi TTTTTTAATA = 6 reads: -107 +92 -72 +85 -38 non-validated
umi TTTTTTACGG = 4 reads: -98 +102 -36 +56 -102 non-validated
umi TTTTTTATTG = 9 reads: -58 +157 -46 +56 -61 +16 non-validated
umi TTTTTTCATG = 3 reads: -196 +141 -57 non-validated
umi TTTTTTCCTT = 4 reads: +19 -79 +123 -173 non-validated
umi TTTTTTCTCA = 5 reads: +39 -90 +70 -5 +13 -1 +35 -1 +30 -110 non-validated
umi TTTTTTGACT = 5 reads: -41 +126 -44 +56 -127 non-validated
umi TTTTTTGCGT = 4 reads: +49 -3 +56 -1 +4 -200 +29 -1 +26 -25 non-validated
umi TTTTTTTAAA = 4 reads: -98 +55 -49 +65 -127 non-validated

GOOD CONTIGS

TIG 1[bases=656]
44-181 ==> 0-137 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=25)
181-307 ==> 140-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=30)
428-474 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=12)
474-656 ==> 0-182 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
junction support: 29 umis using 91 reads
cdr3 = CARGPLVRGILGKGYYLDLW at 383, score = 8 + 6
umis assigned: [0, 1, 5, 6, 13, 15, 16, 17, 20, 21] and 5628 others
of which 5058 are surviving nonsolos
reads assigned: 23682
start codons at 0, 44, 88, 353
confident = true
see deletion of 3 bases at pos 137 on |197|IGHV4/OR15-8|L-REGION+V-REGION|
>vscore_45.1374_77.1%
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTCCTGTCGCAGGGACAGCTGCGAGAGTGGGGCGGAGGCCTGTTGGAGACCTCGCAGACGCTGTCACTCACCTGTTCTGTTTACGGTCGAGTCTTCGCTGGCTCCTTC...

TIG 2[bases=643]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
432-643 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 100 umis using 369 reads
cdr3 = CCSEAGGGVPGLLF at 362, score = 7 + 9
umis assigned: [2, 3, 7, 8, 9, 11, 12, 14, 18, 19] and 10227 others
of which 9644 are surviving nonsolos
reads assigned: 50802
start codons at 38, 192
confident = true

REJECT CONTIGS

TIG 1[bases=524]
13-41 ==> 181-209 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=2)
97-159 ==> 298-360 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=6)
158-196 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
196-370 ==> 0-174 on rc of segment before IGKJ1 exon 1 [len=322] (mis=18)
388-524 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [79, 132, 161, 171, 213, 332, 591, 807, 917, 953] and 202 others
of which 211 are surviving nonsolos
reads assigned: 1893
start codons at 82, 118, 138, 192, 254, 317, 430
confident = false
did not find CDR3
now this is a cell
paired!

ACATATTACTGTGCCAGGGGCCCCTTGGTTCGAGGAATTTTAGGCAAAGGCTATTACCTGGACCTCTGGGGCGAAGGGGCCACCGTCACCGTCTCCGCAG <==> ATCCAGGCTGAGGACGAGGCTGATTATTTCTGCTGCTCAGAAGCGGGTGG...

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1379 = CTCAGAAAGGATGGTC-1

using 218 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 211]
surviving nonsolo ucounts = 1[211]
ids = [3]

====================================================================================

UMI info for barcode CTCAGAAAGGATGGTC-1 contig 1 = GGGGTCACAA...
umi TACTGTCCGT = 202 reads: +394 validated
umi TGCCATACTT = 1 reads: -91 +5 -1 +2 -1X +2 -1 +2 -1 +4 -1 +3 -1 +3 -1 +4 -1 +7 -1 +1 -1 +13 -247 invalidated

GOOD CONTIGS

TIG 1[bases=564]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
394-432 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
432-564 ==> 0-132 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 33 reads
cdr3 = CCSEAGGGVPGLLF at 362, score = 7 + 9
umis assigned: [3, 4]
of which 1 are surviving nonsolos
reads assigned: 202
start codons at 38, 192
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1380 = CTCAGAAAGGCACATG-1

using 44 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 41]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1395 = CTCAGAAAGTCCCACG-1

using 62 reads

====================================================================================

graph has 40 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[55]
surviving nonsolo ucounts = 1[55]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1406 = CTCAGAACAACTGCGC-1

using 537 reads

====================================================================================

graph has 172 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[5, 21, 510]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1410 = CTCAGAACAATGGAAT-1

using 904 reads

====================================================================================

graph has 309 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 9[2^2, 5^3, 9^2, 273, 584]
surviving nonsolo ucounts = 2[273, 584]
ids = [3, 16]

====================================================================================

UMI info for barcode CTCAGAACAATGGAAT-1 contig 1 = GGGGAGGAAC...
umi CATTCACTTG = 242 reads: +385 validated
umi TCTAATGAAC = 514 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=510]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
383-421 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
421-510 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 139 reads
cdr3 = CQQYNNWPPWTF at 357, score = 9 + 8
umis assigned: [3, 16]
of which 2 are surviving nonsolos
reads assigned: 749
start codons at 36, 105, 241, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1415 = CTCAGAACACCGAATT-1

using 165 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[164]
surviving nonsolo ucounts = 1[164]
ids = [1]

====================================================================================

UMI info for barcode CTCAGAACACCGAATT-1 contig 1 = AGCACTAGAA...
umi TAGCTATCAG = 162 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=574]
0-60 ==> 20-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
60-411 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=0)
426-472 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
472-574 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARDGEWLVLDYW at 402, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 161
start codons at 60, 211, 216, 269, 274, 277, 295, 363, 412, 490, 551
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1426 = CTCAGAACAGATGGGT-1

using 233 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 228]
surviving nonsolo ucounts = 1[228]
ids = [1]

====================================================================================

UMI info for barcode CTCAGAACAGATGGGT-1 contig 1 = TCTAGAGAAT...
umi ATATAACATG = 217 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=564]
0-34 ==> 30-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
34-387 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=5)
410-458 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
458-564 ==> 0-106 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 19 reads
cdr3 = CARSLVSYSSSWIFDYW at 376, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 34, 232, 237, 269, 298, 331
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1427 = CTCAGAACAGCAGTTT-1

using 404 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 11, 120, 267]
surviving nonsolo ucounts = 3[11, 120, 267]
ids = [4, 1, 3]

====================================================================================

UMI info for barcode CTCAGAACAGCAGTTT-1 contig 1 = GCTGGGGTCT...
umi AGAAGAGTCC = 113 reads: +388 validated

UMI info for barcode CTCAGAACAGCAGTTT-1 contig 2 = TGGGGGGAGT...
umi CAGTCATGCA = 262 reads: +388 validated
umi CATGTCACTG = 12 reads: -36 +2 -1 +2 -1 +8 -1 +2 -1 +2 -5 +4 -1X +1 -1 +4 -2 +6 -1 +175 -60 +28 -1 +36 -7 invalidated

GOOD CONTIGS

TIG 1[bases=512]
0-41 ==> 1-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=0)
41-393 ==> 0-352 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=0)
391-429 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
429-512 ==> 0-83 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CCSYAGSYTWVF at 365, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 112
start codons at 41, 180, 198, 242, 249, 252, 348, 375
confident = false

TIG 2[bases=555]
31-384 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQSYTTLLTF at 358, score = 9 + 9
umis assigned: [3, 4]
of which 2 are surviving nonsolos
reads assigned: 271
start codons at 31, 37, 93, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 45.1433 = CTCAGAACAGTTCCCT-1

using 1516 reads

====================================================================================

graph has 1104 edges initially, 20 edges after simplification

total ucounts = 304
nonsolo ucounts = 221[2^35, 3^34, 4^23, 5^26, 6^16, 7^10, 8^13, 9^11, 10^14, 11^8, 12^9, 13^7, 14^5, 15, 16^2, 17^3, 18^2, 19, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.19
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ...

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

178.124 seconds used processing barcodes, peak mem = 0.33
