[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.25 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk042-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk042-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk042.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.0 = CTAACTTTCAGTCCCT-1

using 779 reads

====================================================================================

graph has 1128 edges initially, 8 edges after simplification

total ucounts = 384
nonsolo ucounts = 158[2^77, 3^28, 4^23, 5^10, 6^6, 7^3, 8^4, 9^3, 10^2, 12, 25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.6 = CTAACTTTCCATTCTA-1

using 249 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 1[249]
ids = [0]

====================================================================================

UMI info for barcode CTAACTTTCCATTCTA-1 contig 1 = GGCTGGGGTC...
umi ATCACCATCG = 251 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=536]
42-386 ==> 0-344 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=20)
392-430 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
430-536 ==> 0-106 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CSSYISSTTLGF at 366, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 42, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.8 = CTAACTTTCCGGGTGT-1

using 311 reads

====================================================================================

graph has 392 edges initially, 2 edges after simplification

total ucounts = 150
nonsolo ucounts = 59[2^25, 3^18, 4^4, 5^5, 6^2, 7, 8^2, 9, 29]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.10 = CTAACTTTCCTAGAAC-1

using 53 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^2, 6, 8, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.14 = CTAACTTTCGCATGAT-1

using 270 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 266]
surviving nonsolo ucounts = 1[266]
ids = [1]

====================================================================================

UMI info for barcode CTAACTTTCGCATGAT-1 contig 1 = AGTCCCACTC...
umi CCAGATCGGC = 253 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=463]
0-20 ==> 7-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
20-371 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=0)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
411-463 ==> 0-52 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQHNSYPQWTF at 347, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 20, 26, 95, 177, 231, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.23 = CTAACTTTCTCGCTTG-1

using 390 reads

====================================================================================

graph has 200 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 383]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=474]
37-304 ==> 2169-2436 on rc of segment before IGHD2-2 exon 1 [len=2436] (mis=0)
340-403 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
403-474 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [0]
of which 0 are surviving nonsolos
reads assigned: 380
start codons at 59, 65, 106, 360
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.24 = CTAACTTTCTCGGACG-1

using 15 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.32 = CTAACTTTCTTGACGA-1

using 269 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 262]
surviving nonsolo ucounts = 1[262]
ids = [5]

====================================================================================

UMI info for barcode CTAACTTTCTTGACGA-1 contig 1 = GATCAGGACT...
umi TTGCATCCTT = 254 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=521]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
427-521 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CMQALQTPRTF at 366, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.36 = CTAAGACAGAAGCCCA-1

using 827 reads

====================================================================================

graph has 302 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[372, 449]
surviving nonsolo ucounts = 1[449]
ids = [1]

====================================================================================

UMI info for barcode CTAAGACAGAAGCCCA-1 contig 1 = AGTGACTCCT...
umi ATGCCCTCTT = 448 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=518]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=2)
394-447 ==> 10-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
447-518 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CAHSFEVTGIYYGMDVW at 365, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 443
start codons at 20, 64, 243, 246, 326, 335, 404
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.40 = CTAAGACAGAGGTAGA-1

using 95 reads

====================================================================================

graph has 90 edges initially, 4 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2, 3^3, 4, 6^2, 7, 12, 13^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.41 = CTAAGACAGCCCAGCT-1

using 231 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[228]
surviving nonsolo ucounts = 1[228]
ids = [2]

====================================================================================

UMI info for barcode CTAAGACAGCCCAGCT-1 contig 1 = GAAGAGCTGC...
umi GGATGGACCT = 226 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=554]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=13)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQQYGRSPGTF at 357, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 33, 241, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.45 = CTAAGACAGGAATCGC-1

using 276 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 272]
surviving nonsolo ucounts = 1[272]
ids = [3]

====================================================================================

UMI info for barcode CTAAGACAGGAATCGC-1 contig 1 = AGCTTCAGCT...
umi GCTGATTATT = 259 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-544 ==> 0-109 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 257
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.49 = CTAAGACAGGACATTA-1

using 335 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 5, 325]
surviving nonsolo ucounts = 1[325]
ids = [3]

====================================================================================

UMI info for barcode CTAAGACAGGACATTA-1 contig 1 = AAAAACCACA...
umi GGGTAAGCTC = 310 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=574]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-574 ==> 0-88 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 50, 248, 253, 270, 314, 347, 540
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.51 = CTAAGACAGGACTGGT-1

using 315 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[314]
surviving nonsolo ucounts = 1[314]
ids = [1]

====================================================================================

UMI info for barcode CTAAGACAGGACTGGT-1 contig 1 = GCTGGGGTCA...
umi GATTTATCCG = 308 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=575]
0-41 ==> 121-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
41-381 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
435-575 ==> 0-140 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 55 reads
cdr3 = CCSEAGGGVPGLLF at 365, score = 7 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 299
start codons at 41, 195
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.53 = CTAAGACAGGCCATAG-1

using 1009 reads

====================================================================================

graph has 340 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2, 40, 76, 251, 280, 356]
surviving nonsolo ucounts = 5[40, 76, 251, 280, 356]
ids = [4, 0, 8, 9, 2]

====================================================================================

UMI info for barcode CTAAGACAGGCCATAG-1 contig 1 = GGGGACTCCT...
umi ACTCCTCAGC = 76 reads: +421 validated
umi CGCCATGTCT = 40 reads: +347 -11 +8 -1 +3 -1 +43 -7 non-validated
umi GTGAGGCGAG = 251 reads: +421 validated

UMI info for barcode CTAAGACAGGCCATAG-1 contig 2 = GGGACTGATC...
umi CAGTTAGGGC = 350 reads: +397 validated
umi TACATTTCCG = 283 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=623]
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=3)
393-441 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
441-623 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 2 umis using 63 reads
cdr3 = CARRRSPWFGALDYW at 365, score = 7 + 7
umis assigned: [0, 4, 8]
of which 3 are surviving nonsolos
reads assigned: 362
start codons at 20, 64, 243, 246, 326, 335, 385
confident = true

TIG 2[bases=569]
0-36 ==> 0-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=2)
36-396 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=4)
395-433 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
433-569 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 97 reads
cdr3 = CMQALQTLFTF at 372, score = 9 + 8
umis assigned: [2, 9]
of which 2 are surviving nonsolos
reads assigned: 626
start codons at 36, 69, 105, 193, 355, 375, 475
confident = true
now this is a cell
paired!

CCTGTGGACACAGCCACATATTACTGTGCACGCAGGCGGAGCCCATGGTTCGGGGCCCTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACCTTATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.60 = CTAAGACAGTGTTAGA-1

using 388 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 3, 378]
surviving nonsolo ucounts = 1[378]
ids = [2]

====================================================================================

UMI info for barcode CTAAGACAGTGTTAGA-1 contig 1 = GGAGTCAGTC...
umi CTACTGCGGC = 332 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=506]
0-26 ==> 21-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
414-506 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CQQSYRRPITF at 353, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 329
start codons at 26, 32, 88, 101, 237, 336, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.64 = CTAAGACCAACAACCT-1

using 509 reads

====================================================================================

graph has 140 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[179, 328]
surviving nonsolo ucounts = 2[179, 328]
ids = [2, 1]

====================================================================================

UMI info for barcode CTAAGACCAACAACCT-1 contig 1 = ATACTTTCTG...
umi CGCTCAACTA = 178 reads: +439 validated

UMI info for barcode CTAAGACCAACAACCT-1 contig 2 = GGAAAACAGA...
umi CGCAACGCTT = 312 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=533]
0-37 ==> 108-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
37-387 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=15)
425-476 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=7)
476-533 ==> 0-57 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CARDVGTTAASPSLHEDNYFGPW at 376, score = 9 + 6
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 174
start codons at 37, 81, 185, 248, 419
confident = false

TIG 2[bases=568]
40-392 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=4)
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
428-568 ==> 0-140 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CSAWDSSLSAWVF at 361, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 40, 179, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.66 = CTAAGACCAAGCGATG-1

using 361 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 357]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

UMI info for barcode CTAAGACCAAGCGATG-1 contig 1 = GACTCAACAA...
umi GTATGCGACT = 363 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=530]
56-409 ==> 0-353 on |736|IGHV1-8|L-REGION+V-REGION| [len=353] (mis=1)
423-459 ==> 10-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
459-530 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CARVWSYWDW at 398, score = 9 + 7
umis assigned: [1]
of which 0 are surviving nonsolos
reads assigned: 355
start codons at 56, 207, 254, 259, 263, 291, 320, 353, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.74 = CTAAGACCACCCTATC-1

using 797 reads

====================================================================================

graph has 1286 edges initially, 2 edges after simplification

total ucounts = 382
nonsolo ucounts = 161[2^63, 3^40, 4^24, 5^13, 6^9, 7^4, 8, 9^2, 11^2, 12, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.76 = CTAAGACCACGAAACG-1

using 311 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^2, 3, 4^3, 5, 7, 13, 264]
surviving nonsolo ucounts = 1[264]
ids = [12]

====================================================================================

UMI info for barcode CTAAGACCACGAAACG-1 contig 1 = GGAGTCAGAC...
umi TCTATGCCCT = 253 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=482]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=4)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
414-482 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQYNSSPWTF at 353, score = 8 + 8
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 250
start codons at 26, 32, 88, 101, 333, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.77 = CTAAGACCACGGTAAG-1

using 240 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 233]
surviving nonsolo ucounts = 1[233]
ids = [6]

====================================================================================

UMI info for barcode CTAAGACCACGGTAAG-1 contig 1 = ACCCAAAAAC...
umi TTCTGAACCA = 220 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=536]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-536 ==> 0-46 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.80 = CTAAGACCAGAGTGTG-1

using 1123 reads

====================================================================================

graph has 1192 edges initially, 10 edges after simplification

total ucounts = 334
nonsolo ucounts = 151[2^49, 3^31, 4^28, 5^17, 6^13, 7^3, 8^2, 9^4, 11^2, 12, 367]
surviving nonsolo ucounts = 1[367]
ids = [43]

====================================================================================

UMI info for barcode CTAAGACCAGAGTGTG-1 contig 1 = AGGAATCAGA...
umi AGCCAACGGG = 346 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=496]
0-27 ==> 0-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=8)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
415-496 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYNTYPWTF at 354, score = 9 + 8
umis assigned: [43]
of which 1 are surviving nonsolos
reads assigned: 340
start codons at 27, 33, 89, 102, 172, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.83 = CTAAGACCAGCGAACA-1

using 120 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[118]
surviving nonsolo ucounts = 1[118]
ids = [1]

====================================================================================

UMI info for barcode CTAAGACCAGCGAACA-1 contig 1 = ATGGGGAGTG...
umi GATTTTTTGT = 114 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=472]
0-23 ==> 8-31 on |185|IGHV4-34|5'UTR| [len=31] (mis=1)
23-394 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
411-459 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
junction support: 1 umis using 11 reads
cdr3 = CARYFAFVNYYFDKW at 383, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 111
start codons at 0, 23, 44, 88
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.86 = CTAAGACCAGTTCATG-1

using 256 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 6[2, 3, 4^2, 12, 217]
surviving nonsolo ucounts = 1[217]
ids = [3]

====================================================================================

UMI info for barcode CTAAGACCAGTTCATG-1 contig 1 = GGCTGGGGTC...
umi ACAGCGGGCA = 210 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=527]
42-395 ==> 0-353 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
430-527 ==> 0-97 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CSSYTSSSTLVF at 366, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 208
start codons at 42, 199, 243, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.88 = CTAAGACCATACTCTT-1

using 28 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.89 = CTAAGACCATATACCG-1

using 189 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[182]
surviving nonsolo ucounts = 1[182]
ids = [5]

====================================================================================

UMI info for barcode CTAAGACCATATACCG-1 contig 1 = AGCTCTGAGA...
umi TGGACCTACC = 183 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=570]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-570 ==> 0-67 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 11 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 177
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.90 = CTAAGACCATCACGAT-1

using 294 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[294]
surviving nonsolo ucounts = 1[294]
ids = [0]

====================================================================================

UMI info for barcode CTAAGACCATCACGAT-1 contig 1 = GATCAGGACT...
umi GTCATACGCC = 281 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=489]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
427-489 ==> 0-62 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CMQALQTPCSF at 366, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 278
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.91 = CTAAGACCATCTGGTA-1

using 247 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 244]
surviving nonsolo ucounts = 1[244]
ids = [1]

====================================================================================

UMI info for barcode CTAAGACCATCTGGTA-1 contig 1 = GGCTTTCTGA...
umi CTCCATTATC = 231 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=471]
15-392 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=7)
406-454 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
junction support: 1 umis using 24 reads
cdr3 = CARGAAAGTSFDYW at 381, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 15, 24, 36, 80
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.106 = CTAAGACGTCGTTGTA-1

using 1737 reads

====================================================================================

graph has 730 edges initially, 12 edges after simplification

total ucounts = 105
nonsolo ucounts = 43[2^15, 3^6, 4^4, 6^2, 7^2, 9, 12, 24, 35, 107^2, 130, 145, 148, 158, 164, 171, 185, 190]
surviving nonsolo ucounts = 12[24, 35, 107^2, 130, 145, 148, 158, 164, 171, 185, 190]
ids = [62, 70, 53, 86, 89, 43, 39, 90, 95, 8, ...]

====================================================================================

UMI info for barcode CTAAGACGTCGTTGTA-1 contig 1 = GAGCTACAAC...
umi ACAATCGGGG = 170 reads: +400 validated
umi CGAACCGGCA = 149 reads: +400 validated
umi CGGCTAACGG = 148 reads: +400 validated
umi CTCGGCATGC = 188 reads: +400 validated
umi GCGCACGCCG = 26 reads: -393X +7 invalidated
umi TAATTGTGAC = 35 reads: +367 -1 +1 -6 +1 -1 +1 -1 +7 -1 +1 -12 non-validated
umi TAATTTTGCT = 186 reads: +400 validated
umi TGCCATCCCC = 106 reads: +400 validated
umi TGTCCGTACA = 129 reads: +400 validated
umi TTAGTACTCT = 158 reads: +400 validated
umi TTGCAGCTTC = 170 reads: +240 -1XX +159 invalidated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=6)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 9 umis using 189 reads
cdr3 = CHQYHSTPWTF at 369, score = 9 + 8
umis assigned: [8, 39, 43, 51, 62, 70, 71, 86, 89, 90] and 1 others
of which 11 are surviving nonsolos
reads assigned: 1441
start codons at 30, 99, 352, 472
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.107 = CTAAGACGTCTTTCAT-1

using 571 reads

====================================================================================

graph has 238 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[10, 559]
surviving nonsolo ucounts = 1[559]
ids = [3]

====================================================================================

UMI info for barcode CTAAGACGTCTTTCAT-1 contig 1 = TGGGAGGAAT...
umi GGTGATAGAA = 518 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=505]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-505 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 82 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 506
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.113 = CTAAGACGTTCCACGG-1

using 446 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[443]
surviving nonsolo ucounts = 1[443]
ids = [3]

====================================================================================

UMI info for barcode CTAAGACGTTCCACGG-1 contig 1 = GTCAGACCCA...
umi GTATTGCATG = 441 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=544]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=5)
370-408 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CQQYNSPFTF at 350, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 436
start codons at 23, 29, 85, 98, 330, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.115 = CTAAGACGTTGACGTT-1

using 177 reads

====================================================================================

graph has 228 edges initially, 6 edges after simplification

total ucounts = 45
nonsolo ucounts = 33[2^7, 3^4, 4^7, 5^5, 6, 7^2, 8^2, 9^2, 10^2, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.116 = CTAAGACGTTGAGTTC-1

using 350 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 342]
surviving nonsolo ucounts = 1[342]
ids = [2]

====================================================================================

UMI info for barcode CTAAGACGTTGAGTTC-1 contig 1 = GTCAGTCTCA...
umi CAGTCTTTCT = 341 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=550]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=0)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQSYSTPPWTF at 350, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 335
start codons at 23, 29, 85, 98, 234, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.130 = CTAAGACTCCACGACG-1

using 287 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^3, 277]
surviving nonsolo ucounts = 1[277]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=547]
1-82 ==> 5665-5746 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=4)
31-361 ==> 0-330 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=25)
372-411 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 274
start codons at 31, 87, 100, 236, 453
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.140 = CTAAGACTCGCAGGCT-1

using 890 reads

====================================================================================

graph has 322 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 345, 538]
surviving nonsolo ucounts = 2[345, 538]
ids = [6, 4]

====================================================================================

UMI info for barcode CTAAGACTCGCAGGCT-1 contig 1 = CTCAGTTAGG...
umi TCATGGGCTT = 346 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=542]
0-24 ==> 23-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
24-369 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
369-406 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
406-542 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CQQRSNWPLTF at 345, score = 9 + 9
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 341
start codons at 24, 232, 448
confident = false

REJECT CONTIGS

TIG 1[bases=363]
0-80 ==> 5533-5613 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=4)
30-228 ==> 0-198 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=1)
227-363 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 532
start codons at 30, 63, 99, 150, 187, 269
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.148 = CTAAGACTCTGCTTGC-1

using 352 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[348]
surviving nonsolo ucounts = 1[348]
ids = [4]

====================================================================================

UMI info for barcode CTAAGACTCTGCTTGC-1 contig 1 = AGACCCAGTC...
umi GTCTATAGCA = 348 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 161-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
20-371 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=16)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 69 reads
cdr3 = CQQFNSHSYTF at 347, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 343
start codons at 20, 26, 82, 95, 327, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.149 = CTAAGACTCTGTCTAT-1

using 332 reads

====================================================================================

graph has 156 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2, 3^2, 5, 9, 18, 289]
surviving nonsolo ucounts = 1[289]
ids = [7]

====================================================================================

REJECT CONTIGS

TIG 1[bases=579]
0-330 ==> 26-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
330-368 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
368-579 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 298, score = 7 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 128, 131, 182, 281, 308, 332, 500
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.154 = CTAAGACTCTTTCCTC-1

using 782 reads

====================================================================================

graph has 276 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 775]
surviving nonsolo ucounts = 1[775]
ids = [1]

====================================================================================

UMI info for barcode CTAAGACTCTTTCCTC-1 contig 1 = AGAGCTCTGG...
umi ACATCGTACT = 778 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=562]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
387-426 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
426-562 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 123 reads
cdr3 = CQQYGSSDTF at 368, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 764
start codons at 44, 252, 378, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.161 = CTAATGGAGAGGTTAT-1

using 234 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 4, 6, 20, 198]
surviving nonsolo ucounts = 1[198]
ids = [5]

====================================================================================

UMI info for barcode CTAATGGAGAGGTTAT-1 contig 1 = TGAGCGCAGA...
umi TATTAGAATA = 192 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=489]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=0)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
424-489 ==> 0-65 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CGTWDSSLSAGVF at 357, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 189
start codons at 36, 190, 241, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.162 = CTAATGGAGAGGTTGC-1

using 620 reads

====================================================================================

graph has 330 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 179, 434]
surviving nonsolo ucounts = 2[179, 434]
ids = [4, 2]

====================================================================================

UMI info for barcode CTAATGGAGAGGTTGC-1 contig 1 = GAGTCAGACT...
umi GGATGAACAT = 423 reads: +388 validated

UMI info for barcode CTAATGGAGAGGTTGC-1 contig 2 = CTCAGAGAGG...
umi TTGAGATTTG = 181 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=508]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
376-413 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
413-508 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 66 reads
cdr3 = CQQYNSYPLTF at 352, score = 8 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 419
start codons at 25, 31, 87, 100, 236, 239, 332, 455
confident = false

TIG 2[bases=526]
0-75 ==> 4-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
75-428 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=38)
459-505 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=6)
505-526 ==> 0-21 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CARESYVGLYSSTSYPDYW at 417, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 174
start codons at 75, 224, 231, 310, 352, 378, 433
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.167 = CTAATGGAGCCATCGC-1

using 361 reads

====================================================================================

graph has 222 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 7[2^3, 3, 4, 10, 337]
surviving nonsolo ucounts = 1[337]
ids = [7]

====================================================================================

UMI info for barcode CTAATGGAGCCATCGC-1 contig 1 = AGTCTCAGTC...
umi TGATCCAGCT = 344 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 27-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
20-371 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=18)
380-408 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQSKSPPRTF at 347, score = 9 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.170 = CTAATGGAGCCCGAAA-1

using 165 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 157]
surviving nonsolo ucounts = 1[157]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.171 = CTAATGGAGCCGATTT-1

using 1233 reads

====================================================================================

graph has 424 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 3[4, 258, 962]
surviving nonsolo ucounts = 2[258, 962]
ids = [2, 5]

====================================================================================

UMI info for barcode CTAATGGAGCCGATTT-1 contig 1 = GGGAGAGGAG...
umi ACTACCGTCC = 249 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=529]
0-73 ==> 148-221 on |145|IGHV3-53|5'UTR| [len=221] (mis=0)
73-423 ==> 0-350 on |146|IGHV3-53|L-REGION+V-REGION| [len=350] (mis=7)
429-460 ==> 0-31 on |13|IGHD2-15|D-REGION| [len=31] (mis=4)
452-500 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
500-529 ==> 0-29 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARGESVYCSGGSCYSPYW at 412, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 73, 229, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.178 = CTAATGGAGGAGTAGA-1

using 1213 reads

====================================================================================

graph has 1292 edges initially, 8 edges after simplification

total ucounts = 342
nonsolo ucounts = 140[2^50, 3^32, 4^23, 5^15, 6^4, 7^5, 8^2, 9^2, 10, 11, 12, 13, 20, 206, 282]
surviving nonsolo ucounts = 2[206, 282]
ids = [42, 190]

====================================================================================

UMI info for barcode CTAATGGAGGAGTAGA-1 contig 1 = TCTGGCACCA...
umi ATCCGCCCGC = 169 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=467]
0-34 ==> 0-34 on |387|IGLV7-46|5'UTR| [len=34] (mis=0)
34-385 ==> 0-351 on |388|IGLV7-46|L-REGION+V-REGION| [len=351] (mis=12)
381-419 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
419-467 ==> 0-48 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CLVSYSGAWVF at 358, score = 8 + 8
umis assigned: [42]
of which 1 are surviving nonsolos
reads assigned: 169
start codons at 34, 242, 341
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.188 = CTAATGGAGTACTTGC-1

using 167 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 4, 5, 154]
surviving nonsolo ucounts = 1[154]
ids = [5]

====================================================================================

UMI info for barcode CTAATGGAGTACTTGC-1 contig 1 = GTCAGTCCCA...
umi TCGCTTTTAA = 134 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=484]
0-23 ==> 35-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
23-355 ==> 0-332 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=24)
373-411 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
411-484 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQHLVTHPISF at 350, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 134
start codons at 23, 29, 234, 237, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.189 = CTAATGGAGTAGATGT-1

using 150 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3^2, 138]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 42.198 = CTAATGGCAACACCTA-1

using 230 reads

====================================================================================

graph has 142 edges initially, 18 edges after simplification

total ucounts = 10
nonsolo ucounts = 9[2, 6, 7, 9, 11^2, 17, 21, 145]
surviving nonsolo ucounts = 2[11, 145]
ids = [3, 7]

====================================================================================

UMI info for barcode CTAATGGCAACACCTA-1 contig 1 = TGGGGAGGAA...
umi CCTCTAGTGC = 6 reads: -83 +1 -1 +1 -1 +5 -1X +3 -1XX +3 -1XX +11 -1XX +34 -2XX +1 -2XX +1 -2XX +15 -1XX +2 -1XX +25 -47 +23 -1X +5 -1X +17 -2X +2 -1X +4 -77 invalidated
umi TCAATAGCCC = 138 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=450]
0-37 ==> 10-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
37-370 ==> 0-333 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=14)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
416-450 ==> 0-34 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQERGGWWTF at 358, score = 9 + 8
umis assigned: [3, 7]
of which 2 are surviving nonsolos
reads assigned: 142
start codons at 37, 245
confident = false
NOT paired!

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BARCODE 42.204 = CTAATGGCACCAGTTA-1

using 620 reads

====================================================================================

graph has 282 edges initially, 24 edges after simplification

total ucounts = 16
nonsolo ucounts = 6[2^2, 3, 10, 293, 300]
surviving nonsolo ucounts = 2[293, 300]
ids = [13, 0]

====================================================================================

UMI info for barcode CTAATGGCACCAGTTA-1 contig 1 = AGCTGTGGTA...
umi AACGTCACTC = 298 reads: +388 validated

UMI info for barcode CTAATGGCACCAGTTA-1 contig 2 = GTCTGGGCCT...
umi TTAGACGTTC = 274 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=562]
0-40 ==> 16-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
40-391 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=5)
390-428 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
428-562 ==> 0-134 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CAAWDDSLSGYVF at 361, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 40, 194, 198, 251, 254, 344, 369, 374, 392
confident = false

TIG 2[bases=555]
0-39 ==> 212-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
39-371 ==> 0-332 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=36)
383-421 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
421-555 ==> 0-134 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQVWDRSRDHVVF at 354, score = 6 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 39, 88, 100, 123, 337, 382
confident = false
NOT paired!
sorting bam, mem = 0.03
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk042-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk042-ucc6a7a72ce/files/contig_bam.bam

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4.531 seconds used processing barcodes, peak mem = 0.23
