[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.33 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk019-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk019-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk019.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.0 = CACCACTCACAACGCC-1

using 672 reads

====================================================================================

graph has 224 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 2[326, 338]
surviving nonsolo ucounts = 2[326, 338]
ids = [3, 5]

====================================================================================

UMI info for barcode CACCACTCACAACGCC-1 contig 1 = GAGTCAGTCC...
umi GCAAACTACC = 323 reads: +388 validated

UMI info for barcode CACCACTCACAACGCC-1 contig 2 = GGAGTCTCCC...
umi CCCACTTGGG = 320 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=477]
0-25 ==> 6-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
25-376 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
376-413 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
413-477 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQYDNLRLTF at 352, score = 9 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 25, 31, 87, 100, 239, 362, 455
confident = false

TIG 2[bases=474]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=3)
426-474 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
junction support: 1 umis using 22 reads
cdr3 = CARSEEGYGAFDYW at 401, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 316
start codons at 59, 233, 257, 392
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.12 = CACCACTCACGGCTAC-1

using 48 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[2^7, 7, 8, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.20 = CACCACTCAGATGAGC-1

using 220 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 3, 210]
surviving nonsolo ucounts = 1[210]
ids = [6]

====================================================================================

UMI info for barcode CACCACTCAGATGAGC-1 contig 1 = GGGGTCTCAG...
umi TTAAGACTCG = 211 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=512]
0-38 ==> 4-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=0)
38-382 ==> 0-344 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=20)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
426-512 ==> 0-86 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CCSHAGSYIWVF at 362, score = 8 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 205
start codons at 38, 174, 177, 195, 249, 345, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.26 = CACCACTCAGCTGTAT-1

using 208 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[204]
surviving nonsolo ucounts = 1[204]
ids = [3]

====================================================================================

UMI info for barcode CACCACTCAGCTGTAT-1 contig 1 = AGCTTCAGCT...
umi TGACTGGGTT = 207 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=32)
397-435 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
435-552 ==> 0-117 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CAAWDVSLNGYVF at 368, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 202
start codons at 47, 351, 376, 381, 393, 399
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.28 = CACCACTCAGGAACGT-1

using 12 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.36 = CACCACTCATAAGACA-1

using 458 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 158, 290]
surviving nonsolo ucounts = 2[158, 290]
ids = [0, 5]

====================================================================================

UMI info for barcode CACCACTCATAAGACA-1 contig 1 = AGCTCAGGAA...
umi CAATGAATGC = 153 reads: +388 validated

UMI info for barcode CACCACTCATAAGACA-1 contig 2 = ATCACATAAC...
umi TCTCGCTACT = 281 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=543]
0-33 ==> 53-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
33-370 ==> 0-337 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=6)
383-421 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
421-543 ==> 0-122 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQSFGSNTRVF at 360, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 151
start codons at 33, 96, 187, 238
confident = false

TIG 2[bases=504]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=3)
446-494 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
junction support: 1 umis using 18 reads
cdr3 = CARSDLVGKGGIAVAVPFDYW at 400, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 58, 209, 256, 355
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.39 = CACCACTCATATACCG-1

using 286 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 280]
surviving nonsolo ucounts = 1[280]
ids = [1]

====================================================================================

UMI info for barcode CACCACTCATATACCG-1 contig 1 = GGGACACTTC...
umi CCGAATGTCA = 276 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=448]
41-392 ==> 0-351 on |244|IGKV1D-13|L-REGION+V-REGION| [len=351] (mis=6)
392-429 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
429-448 ==> 0-19 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQFNNYPLTF at 368, score = 10 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 41, 47, 103, 252, 255
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.40 = CACCACTCATCACGTA-1

using 685 reads

====================================================================================

graph has 228 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 680]
surviving nonsolo ucounts = 1[680]
ids = [1]

====================================================================================

UMI info for barcode CACCACTCATCACGTA-1 contig 1 = GAGGAATCAG...
umi GTACCATTGG = 677 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 113 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 669
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.41 = CACCACTCATCAGTCA-1

using 544 reads

====================================================================================

graph has 168 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 4, 11, 170, 351]
surviving nonsolo ucounts = 2[170, 351]
ids = [4, 5]

====================================================================================

UMI info for barcode CACCACTCATCAGTCA-1 contig 1 = GGGAGTCTCA...
umi CTCGGTCTCA = 340 reads: +388 validated

UMI info for barcode CACCACTCATCAGTCA-1 contig 2 = AGTGCTTTCT...
umi CGCCATAGTA = 166 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=500]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=2)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=2)
373-411 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
411-500 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQSYSTPRTF at 350, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 23, 29, 85, 98, 234, 453
confident = false

TIG 2[bases=479]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=8)
405-453 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
453-479 ==> 0-26 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARAHGDYYTLLDYW at 377, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 166
start codons at 17, 38, 82, 168, 368
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.46 = CACCACTCATGTCGAT-1

using 189 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 10, 173]
surviving nonsolo ucounts = 1[173]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.58 = CACCACTGTCAACTGT-1

using 9 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.64 = CACCACTGTCCCGACA-1

using 6579 reads

====================================================================================

graph has 6228 edges initially, 132 edges after simplification

total ucounts = 1216
nonsolo ucounts = 876[2^130, 3^129, 4^96, 5^82, 6^91, 7^54, 8^61, 9^34, 10^41, 11^34, 12^29, 13^18, 14^26, 15^16, 16^8, 17^6, 18^5, 19^5, 20, 21^3, 22, 23, 26, 72, 74, 147, 248]
surviving nonsolo ucounts = 2[147, 248]
ids = [847, 116]

====================================================================================

UMI info for barcode CACCACTGTCCCGACA-1 contig 1 = AGTCAGTCTC...
umi ACCGCTAATG = 259 reads: +55 -1XX +97 -2XX +59 -1XX +8 -1XX +84 -1XX +3 -1XX +31 -1XX +1 -2XX +2 -4XX +1 -2XX +1 -1XX +1 -1XX +1 -1XX +2 -1XX +5 -1XX +5 -4XX +8 -1XX +4 invalidated
umi GTTGTCCTCA = 139 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=507]
24-377 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=2)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
418-507 ==> 0-89 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQQSYSTPGMYTF at 351, score = 9 + 8
umis assigned: [116, 847]
of which 2 are surviving nonsolos
reads assigned: 363
start codons at 24, 30, 86, 99, 235, 378, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.67 = CACCACTGTCGACTGC-1

using 20 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.68 = CACCACTGTCGCGTGT-1

using 102 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 5[3, 8, 9, 26, 47]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.76 = CACCACTGTGCTTCTC-1

using 337 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 4[2, 3, 4, 320]
surviving nonsolo ucounts = 1[320]
ids = [3]

====================================================================================

UMI info for barcode CACCACTGTGCTTCTC-1 contig 1 = GGAGGAACTG...
umi AGCCAGTTCG = 321 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=552]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-340 ==> 0-306 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=25)
379-416 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQYNKWPLTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 316
start codons at 34, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.77 = CACCACTGTGGTACAG-1

using 629 reads

====================================================================================

graph has 194 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[3, 4, 62, 227, 327]
surviving nonsolo ucounts = 2[227, 327]
ids = [1, 7]

====================================================================================

UMI info for barcode CACCACTGTGGTACAG-1 contig 1 = GAGGAGTCAG...
umi AGCGATGGCT = 210 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=474]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=18)
374-413 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
413-474 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CQQSYSTPSF at 355, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 28, 34, 90, 103, 239, 455
confident = false

REJECT CONTIGS

TIG 1[bases=352]
1-207 ==> 147-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=0)
207-228 ==> 0-21 on |32|IGHD6-13|D-REGION| [len=21] (mis=1)
235-281 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
281-352 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
cdr3 = CARDGIAAAGTVVDLYYW at 196, score = 9 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 323
start codons at 5, 10, 52, 57, 74, 151
confident = false
VJ delta = 6
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.81 = CACCACTGTTATGCGT-1

using 517 reads

====================================================================================

graph has 218 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[203, 309]
surviving nonsolo ucounts = 2[203, 309]
ids = [3, 5]

====================================================================================

UMI info for barcode CACCACTGTTATGCGT-1 contig 1 = GAGCTACAAC...
umi CCGCTCGCCT = 197 reads: +400 validated
umi GTACTATGCT = 300 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=498]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
430-498 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 52 reads
cdr3 = CQQYYSTPRTF at 369, score = 9 + 8
umis assigned: [3, 5]
of which 2 are surviving nonsolos
reads assigned: 492
start codons at 30, 99, 352, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.90 = CACCACTGTTGACGTT-1

using 26 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[8, 18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.95 = CACCACTGTTGTCTTT-1

using 155 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[5, 145]
surviving nonsolo ucounts = 1[145]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.97 = CACCACTGTTTCGCTC-1

using 17 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.99 = CACCACTGTTTGGCGC-1

using 276 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 8[2^2, 3^2, 6, 8, 12, 235]
surviving nonsolo ucounts = 1[235]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.105 = CACCACTTCAACCAAC-1

using 257 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 245]
surviving nonsolo ucounts = 1[245]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.124 = CACCACTTCATTCACT-1

using 305 reads

====================================================================================

graph has 166 edges initially, 4 edges after simplification

total ucounts = 29
nonsolo ucounts = 13[2^5, 3^2, 4, 6, 9, 11, 14, 229]
surviving nonsolo ucounts = 1[229]
ids = [20]

====================================================================================

REJECT CONTIGS

TIG 1[bases=567]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
10-64 ==> 5937-5991 on segment before IGLV2-5 exon 1 [len=6137] (mis=2)
19-85 ==> 5809-5875 on segment before IGLV2-34 exon 1 [len=6000] (mis=4)
38-247 ==> 0-209 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0)
247-376 ==> 211-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0) [SHIFT!]
392-430 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
430-567 ==> 0-137 on |307|IGLC3|C-REGION| [len=317] (mis=0)
cdr3 = CCSYAGSSTPHWVF at 360, score = 8 + 8
umis assigned: [20]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 38, 177, 239, 370
confident = false
not full
frameshifted full length stopped transcript of length 567
VJ delta = 8
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.128 = CACCACTTCCAGAAGG-1

using 326 reads

====================================================================================

graph has 176 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 9[2^2, 3^3, 4, 7, 9, 282]
surviving nonsolo ucounts = 1[282]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.144 = CACCACTTCGGCGCTA-1

using 58 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 4, 50]
surviving nonsolo ucounts = 1[50]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=417]
0-334 ==> 6-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
350-388 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
388-417 ==> 0-29 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CCSEAGGGVPGLLF at 318, score = 7 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 47
start codons at 148
confident = false
not full
VJ delta = 6
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.147 = CACCACTTCGTTGCCT-1

using 690 reads

====================================================================================

graph has 250 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 3, 9, 14, 655]
surviving nonsolo ucounts = 1[655]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.151 = CACCACTTCTAACTTC-1

using 30 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[30]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.152 = CACCACTTCTACCTGC-1

using 15 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.158 = CACCACTTCTCTAAGG-1

using 424 reads

====================================================================================

graph has 160 edges initially, 4 edges after simplification

total ucounts = 15
nonsolo ucounts = 8[2^2, 3^2, 4^2, 121, 278]
surviving nonsolo ucounts = 2[121, 278]
ids = [4, 13]

====================================================================================

UMI info for barcode CACCACTTCTCTAAGG-1 contig 1 = CAGAGCTCTG...
umi TTGAGCTTAC = 270 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=477]
0-45 ==> 52-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
45-390 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
427-477 ==> 0-50 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYNNWPRTF at 366, score = 9 + 8
umis assigned: [13]
of which 1 are surviving nonsolos
reads assigned: 264
start codons at 45, 114, 250, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.164 = CACCACTTCTTCATGT-1

using 207 reads

====================================================================================

graph has 124 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 12[2^4, 3, 4, 5, 6^2, 7, 14, 143]
surviving nonsolo ucounts = 1[143]
ids = [18]

====================================================================================

UMI info for barcode CACCACTTCTTCATGT-1 contig 1 = CAATGAGGCT...
umi TCACATGCCG = 141 reads: -1 +393 non-validated

GOOD CONTIGS

TIG 1[bases=446]
2-362 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=4)
357-396 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
396-446 ==> 0-50 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CMQALQTSSF at 338, score = 9 + 7
umis assigned: [18]
of which 1 are surviving nonsolos
reads assigned: 139
start codons at 2, 35, 71, 159, 321, 341, 438
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.173 = CACCAGGAGACTGTAA-1

using 340 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 6, 7, 322]
surviving nonsolo ucounts = 1[322]
ids = [0]

====================================================================================

UMI info for barcode CACCAGGAGACTGTAA-1 contig 1 = TGGGGGATCA...
umi ACCTTCCGTA = 305 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=461]
35-361 ==> 0-326 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=11)
394-432 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
432-461 ==> 0-29 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CMNTLPGGFTF at 371, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 300
start codons at 35, 68, 104, 192, 354, 374
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.175 = CACCAGGAGAGCTGGT-1

using 28 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 5, 6, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.177 = CACCAGGAGAGTCTGG-1

using 246 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 239]
surviving nonsolo ucounts = 1[239]
ids = [2]

====================================================================================

UMI info for barcode CACCAGGAGAGTCTGG-1 contig 1 = GCTCTGCTTC...
umi CGTGCTTGTT = 231 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=544]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-544 ==> 0-99 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 228
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.181 = CACCAGGAGATGGGTC-1

using 31 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^4, 3, 15]
surviving nonsolo ucounts = 1[15]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.184 = CACCAGGAGCACACAG-1

using 250 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^2, 3, 5, 231]
surviving nonsolo ucounts = 1[231]
ids = [4]

====================================================================================

UMI info for barcode CACCAGGAGCACACAG-1 contig 1 = AGTCTGGGCC...
umi CACGATATCG = 219 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=519]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-373 ==> 0-333 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=29)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
422-519 ==> 0-97 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQVWDSDGHYVVF at 355, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 217
start codons at 40, 101, 170, 338, 374, 383
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.187 = CACCAGGAGCCAGGAT-1

using 361 reads

====================================================================================

graph has 190 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 6[2^3, 3, 5, 337]
surviving nonsolo ucounts = 1[337]
ids = [10]

====================================================================================

UMI info for barcode CACCAGGAGCCAGGAT-1 contig 1 = AGGAGTCAGT...
umi TAACTTATAA = 308 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=502]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-502 ==> 0-87 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CHQYESVPYTF at 354, score = 9 + 7
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 27, 33, 89, 102, 241, 337, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.191 = CACCAGGAGGAGTACC-1

using 472 reads

====================================================================================

graph has 166 edges initially, 26 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[200, 269]
surviving nonsolo ucounts = 2[200, 269]
ids = [1, 0]

====================================================================================

UMI info for barcode CACCAGGAGGAGTACC-1 contig 1 = TGGGGAGGAA...
umi ATGACAACGG = 258 reads: +118 -1XX +263 invalidated

UMI info for barcode CACCAGGAGGAGTACC-1 contig 2 = TGGGGAGGAA...
umi CGGGACCAAC = 194 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=483]
0-37 ==> 60-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
37-343 ==> 0-306 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=24)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
419-483 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQYNKWPLTF at 358, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 37, 242, 461
confident = false

TIG 2[bases=489]
37-385 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=9)
387-425 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
425-489 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CLQSSTSPPPTF at 361, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 192
start codons at 37, 245, 467
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.198 = CACCAGGAGGTCGGAT-1

using 10909 reads

====================================================================================

graph has 6174 edges initially, 112 edges after simplification

total ucounts = 890
nonsolo ucounts = 473[2^145, 3^86, 4^66, 5^57, 6^26, 7^18, 8^17, 9^11, 10^8, 11^4, 12^3, 13, 14, 17, 21, 34, 36, 63, 109, 111, 116, 127, 159, 185, 195, 205, 226, 238, 259, 270, 274, 300, 301^2, 306, 308, 323, 453, 494, 618, 717, 949, 976]
surviving nonsolo ucounts = 25[63, 109, 111, 116, 127, 159, 185, 195, 205, 226, 238, 270, 274, 300, 301^2, 306, 308, 323, 453, 494, 618, 717, 949, 976]
ids = [773, 124, 241, 641, 35, 405, 418, 703, 16, 635, ...]

====================================================================================

UMI info for barcode CACCAGGAGGTCGGAT-1 contig 1 = AGTCTGGGCC...
umi CACTATGCCC = 492 reads: -263 +119 non-validated
umi GCCAATAGCG = 276 reads: +382 validated
umi GGCTAAGGCT = 237 reads: +382 validated
umi GTGCCTGCTT = 225 reads: +382 validated
umi GTGGGCCGCA = 300 reads: +382 validated
umi TAACAACGAA = 885 reads: -382 non-validated
umi TAGTTAACGG = 180 reads: +382 validated
umi TCGCTCCAAT = 872 reads: -382 non-validated
umi TTATCGCTTG = 276 reads: +382 validated

UMI info for barcode CACCAGGAGGTCGGAT-1 contig 2 = GAGAGAGGAG...
umi AACATTCGCC = 204 reads: +442 validated
umi AAGCTGAGGC = 125 reads: +442 validated
umi AAGTGGATCT = 188 reads: +51 -1XX +13 -2XX +2 -1XX +15 -2XX +1 -1XX +9 -1XX +27 -1XX +4 -1XX +15 -4XX +9 -1XX +43 -1XX +6 -1XX +8 -2XX +5 -1XX +2 -1XX +1 -1XX +1 -1XX +4 -203 invalidated
umi CTAGGTATCC = 97 reads: +51 -1XX +13 -2XX +2 -1XX +15 -2XX +1 -1XX +9 -1XX +27 -1XX +4 -1XX +15 -4XX +9 -1XX +43 -1XX +6 -1XX +8 -2XX +5 -1XX +2 -1XX +1 -1XX +1 -1XX +5 -202 invalidated
umi CTCTGTTCGA = 182 reads: +435 -3 +4 non-validated
umi GTGTCAACCG = 76 reads: +51 -1XX +13 -2XX +2 -1XX +15 -2XX +1 -1XX +9 -1XX +27 -1XX +4 -1XX +15 -4XX +9 -1XX +43 -1XX +6 -1XX +8 -2XX +5 -1XX +2 -1XX +1 -1XX +1 -1X +3 -204 invalidated

UMI info for barcode CACCAGGAGGTCGGAT-1 contig 3 = GGGGAGTCAG...
umi GGTAACCGGC = 301 reads: +388 validated
umi TAAATGAGGA = 39 reads: -241 +2 -1X +1 -1X +1 -1 +1 -1X +1 -1X +6 -1XX +16 -1XX +20 -1XX +2 -2XX +1 -1XX +5 -1XX +8 -1XX +5 -1XX +8 -29 +2 -3X +7 -1X +1 -1X +4 -1X +3 -1X +2 -1XX invalidated
umi TAGAGGAGGC = 306 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=633]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-391 ==> 0-351 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=15)
384-422 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
422-633 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 7 umis using 287 reads
cdr3 = CQVWDSSSDHWVF at 355, score = 8 + 8
umis assigned: [265, 503, 567, 635, 640, 665, 703, 765, 847]
of which 9 are surviving nonsolos
reads assigned: 3631
start codons at 40, 101, 242, 338
confident = true

TIG 2[bases=572]
0-73 ==> 6-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=2)
73-392 ==> 0-319 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=26)
467-515 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
515-572 ==> 0-57 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 2 umis using 33 reads
cdr3 = CVKGPKRFLWDSTAWSSPYFESW at 415, score = 8 + 6
umis assigned: [16, 35, 44, 405, 418, 641]
of which 6 are surviving nonsolos
reads assigned: 842
start codons at 73, 224, 229, 376
confident = true

TIG 3[bases=552]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=27)
377-416 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 107 reads
cdr3 = CQQGYSIPYTF at 355, score = 9 + 8
umis assigned: [587, 664, 694]
of which 2 are surviving nonsolos
reads assigned: 638
start codons at 28, 34, 90, 103, 458
confident = true

REJECT CONTIGS

TIG 1[bases=566]
0-80 ==> 3254-3334 on rc of segment before IGKV2-24 exon 2 [len=3774] (mis=4)
0-80 ==> 3251-3331 on segment before IGKV2D-23 exon 1 [len=3772] (mis=4)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=40)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CMQATQFPKLTF at 366, score = 9 + 9
umis assigned: [124, 241, 327, 630, 872]
of which 5 are surviving nonsolos
reads assigned: 1538
start codons at 63, 99, 187, 349, 369, 472
confident = false
not full
VJ delta = 13
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.201 = CACCAGGAGGTTACCT-1

using 19 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.203 = CACCAGGAGTACGTTC-1

using 354 reads

====================================================================================

graph has 168 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[4, 345]
surviving nonsolo ucounts = 1[345]
ids = [4]

====================================================================================

UMI info for barcode CACCAGGAGTACGTTC-1 contig 1 = GAGAGCATCA...
umi GCGTCCAGCC = 318 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=517]
0-64 ==> 0-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
64-417 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=7)
440-488 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
488-517 ==> 0-29 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CARSLVSYSSSWIFDYW at 406, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 64, 262, 267, 299, 328, 361
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.214 = CACCAGGAGTGTGAAT-1

using 111 reads

====================================================================================

graph has 104 edges initially, 8 edges after simplification

total ucounts = 30
nonsolo ucounts = 17[2^4, 3^6, 5, 10^2, 11^2, 12, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.226 = CACCAGGCACATGTGT-1

using 318 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 4, 308]
surviving nonsolo ucounts = 1[308]
ids = [0]

====================================================================================

UMI info for barcode CACCAGGCACATGTGT-1 contig 1 = GGAATCAGTC...
umi ATGATGGATA = 292 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=496]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-496 ==> 0-82 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.235 = CACCAGGCACTTCTGC-1

using 284 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[281]
surviving nonsolo ucounts = 1[281]
ids = [3]

====================================================================================

UMI info for barcode CACCAGGCACTTCTGC-1 contig 1 = ACTTTCTGAG...
umi TAGTATCCTT = 282 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=559]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=17)
400-450 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
450-559 ==> 0-109 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 35 reads
cdr3 = CARDFPGNYFTFDIW at 374, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 35, 79, 159, 229, 431
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.243 = CACCAGGCAGGCGATA-1

using 258 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[258]
surviving nonsolo ucounts = 1[258]
ids = [0]

====================================================================================

UMI info for barcode CACCAGGCAGGCGATA-1 contig 1 = CTGGGCCTCA...
umi GAGTATAATC = 255 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=572]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-371 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=18)
375-413 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
413-572 ==> 0-159 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQAWDSTEVVF at 352, score = 6 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 37, 42, 331, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.246 = CACCAGGCAGGTTTCA-1

using 632 reads

====================================================================================

graph has 256 edges initially, 4 edges after simplification

total ucounts = 13
nonsolo ucounts = 2[290, 331]
surviving nonsolo ucounts = 2[290, 331]
ids = [8, 6]

====================================================================================

UMI info for barcode CACCAGGCAGGTTTCA-1 contig 1 = GGAGTCAGTC...
umi CGTAGCGCGC = 318 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=498]
0-26 ==> 5-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
26-377 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
417-498 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYDNLPPYTF at 353, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 314
start codons at 26, 32, 88, 101, 240, 363, 459
confident = false

REJECT CONTIGS

TIG 1[bases=492]
0-72 ==> 7-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
0-72 ==> 5928-6000 on rc of segment after IGHV3-7 exon 1 [len=6000] (mis=0)
40-111 ==> 6506-6577 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=3)
72-425 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=4)
448-492 ==> 13-57 on |58|IGHJ6|J-REGION| [len=61] (mis=0)
cdr3 = CARVIWFGESPNYMDVW at 414, score = 9 + 7
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 72, 228, 289, 307, 375, 428, 453
confident = false
VJ delta = 21
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.257 = CACCAGGCATTGTGCA-1

using 233 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 3, 224]
surviving nonsolo ucounts = 1[224]
ids = [3]

====================================================================================

UMI info for barcode CACCAGGCATTGTGCA-1 contig 1 = GCTCTGCTTC...
umi GGTGCGCCGT = 223 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=576]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
442-576 ==> 0-134 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CQSYDTSLSGSVF at 375, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 218
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 19.260 = CACCAGGGTACTTAGC-1

using 420 reads

====================================================================================

graph has 194 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 4[2, 8, 195, 206]
surviving nonsolo ucounts = 2[195, 206]
ids = [10, 8]

====================================================================================

UMI info for barcode CACCAGGGTACTTAGC-1 contig 1 = GCTCTGCTTC...
umi CGTGTATTTG = 199 reads: +394 validated
umi TGATGTCCTG = 188 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=565]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-565 ==> 0-120 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 77 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [8, 10]
of which 2 are surviving nonsolos
reads assigned: 382
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!
sorting bam, mem = 0.06
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk019-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk019-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

5.680 seconds used processing barcodes, peak mem = 0.23
