[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.34 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk014-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk014-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk014.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.0 = AGTGGGACAAGGTGTG-1

using 644 reads

====================================================================================

graph has 340 edges initially, 30 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^3, 256, 375]
surviving nonsolo ucounts = 2[256, 375]
ids = [11, 0]

====================================================================================

UMI info for barcode AGTGGGACAAGGTGTG-1 contig 1 = AGTCAGTCTC...
umi ACCTGTTCCA = 375 reads: +391 validated

UMI info for barcode AGTGGGACAAGGTGTG-1 contig 2 = TGGGGAGGAA...
umi TTCTGTCTAG = 260 reads: +110 -1XX +13 -1XX +263 invalidated

GOOD CONTIGS

TIG 1[bases=551]
24-377 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=1)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CQQSYSTPSLTF at 351, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 368
start codons at 24, 30, 86, 99, 235, 457
confident = false

TIG 2[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=17)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 252
start codons at 32, 38, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.5 = AGTGGGACAATGACCT-1

using 289 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 11, 274]
surviving nonsolo ucounts = 1[274]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGACAATGACCT-1 contig 1 = GATCAGGACT...
umi AATCATGTAA = 239 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=481]
0-30 ==> 0-30 on |266|IGKV2-30|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=2)
386-424 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
424-481 ==> 0-57 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CMQGTLWPTF at 366, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 234
start codons at 30, 63, 91, 99, 187, 349, 369, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.6 = AGTGGGACAATGTTGC-1

using 355 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 347]
surviving nonsolo ucounts = 1[347]
ids = [4]

====================================================================================

UMI info for barcode AGTGGGACAATGTTGC-1 contig 1 = GGAGTCAGTC...
umi TAAATATTCT = 345 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 32-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
26-358 ==> 0-332 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=24)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CQHLVTHPISF at 353, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 344
start codons at 26, 32, 237, 240, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.10 = AGTGGGACACAGACAG-1

using 1768 reads

====================================================================================

graph has 544 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 10, 1752]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=322]
46-77 ==> 3884-3915 on segment before IGLJ4 exon 1 [len=3915] (mis=6)
53-82 ==> 4604-4633 on segment before IGLCOR22-1 exon 1 [len=6000] (mis=1)
73-111 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
111-322 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [3]
of which 0 are surviving nonsolos
reads assigned: 1729
start codons at 54
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.15 = AGTGGGACACCCAGTG-1

using 283 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[9, 271]
surviving nonsolo ucounts = 1[271]
ids = [3]

====================================================================================

UMI info for barcode AGTGGGACACCCAGTG-1 contig 1 = GCTCTGCTTC...
umi TCTCCTTGTA = 270 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=656]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-656 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 266
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.16 = AGTGGGACACGAAACG-1

using 17121 reads

====================================================================================

graph has 5914 edges initially, 68 edges after simplification

total ucounts = 466
nonsolo ucounts = 235[2^68, 3^39, 4^23, 5^15, 6^6, 7^6, 8^11, 9^2, 10^3, 13, 14, 15, 17, 24, 43, 44, 52, 71, 85, 89, 133, 139, 149, 151, 156, 166, 173, 183, 194, 211, 214, 225, 230^2, 232^2, 238, 251, 258, 260, 263, 264, 268, 272, 279, 285, 287, 290, 293, 301, 306, 315^2, 317, 328^2, 340, 343^2, 344, 349, 351, 352, 393, 401, 417, 464, 591, 601, 841, 923]
surviving nonsolo ucounts = 54[71, 85, 89, 133, 139, 149, 151, 156, 166, 173, 183, 194, 211, 214, 225, 230^2, 232^2, 238, 251, 258, 260, 263, 264, 268, 272, 279, 285, 287, 290, 293, 301, 306, 315^2, 317, 328^2, 340, 343^2, 344, 349, 351, 352, 393, 401, 417, 464, 591, 601, 841, 923]
ids = [365, 212, 77, 225, 254, 50, 449, 10, 283, 113, ...]

====================================================================================

UMI info for barcode AGTGGGACACGAAACG-1 contig 1 = ATACTTTCTG...
umi AATCATCGGC = 176 reads: -347X +1 -1X +1 -1X +2 -7X +1 -5X +2 -2X +2 -2X +2 -2X +37 invalidated
umi AATGTTTGGC = 329 reads: -244X +171 invalidated
umi AGAAGTGGTC = 122 reads: +401 -14 non-validated
umi AGTTTGATTC = 84 reads: -389 +26 non-validated
umi ATTGGACCCT = 129 reads: +321 -3X +91 invalidated
umi GGCAGTCCTA = 396 reads: +415 validated
umi GGCCTCTCGG = 469 reads: +415 validated
umi GGTGGCACTT = 168 reads: +415 validated
umi GTCCTGTTCA = 203 reads: +415 validated
umi GTGACCTCCT = 262 reads: +386 -1 +28 non-validated
umi TAACTTGTGC = 353 reads: +415 validated
umi TCTGAGGTTT = 398 reads: +415 validated
umi TGACGCTTCG = 267 reads: +415 validated
umi TGTCCCCAAG = 849 reads: +415 validated

UMI info for barcode AGTGGGACACGAAACG-1 contig 2 = AGAGCTCTGG...
umi AGCAATGCCT = 601 reads: -356X +1 -3XX +34 invalidated
umi ATCAAAACGC = 281 reads: +394 validated
umi ATGCGTCGCG = 342 reads: +394 validated
umi CCCAGCATAC = 592 reads: -360X +34 invalidated
umi CCCTCCTTTA = 274 reads: +394 validated
umi GATTCTACCT = 315 reads: +394 validated
umi GATTTACCCA = 314 reads: +394 validated
umi GTTAGGCTTT = 196 reads: -330X +1 -2XX +1 -3XX +1 -7XX +1 -3XX +1 -6XX +1 -3XX +34 invalidated
umi TCCACACGAA = 71 reads: -240 +154 non-validated
umi TCTATCTCTG = 226 reads: +394 validated
umi TCTCCCCACC = 345 reads: +394 validated
umi TGAGGTTCCC = 928 reads: -346 +1 -2XX +1 -6XX +1 -3XX +34 invalidated

UMI info for barcode AGTGGGACACGAAACG-1 contig 3 = GGAGCTCTAC...
umi CCTAATAGTC = 263 reads: +400 validated
umi CGACATCTAT = 313 reads: +400 validated
umi CTTGACTGGC = 136 reads: +400 validated
umi TATCGCCTTT = 299 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=554]
0-37 ==> 27-64 on |191|IGHV4-4|5'UTR| [len=64] (mis=2)
37-390 ==> 0-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=0)
415-452 ==> 9-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
452-554 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 11 umis using 383 reads
cdr3 = CARDMEAVGPGAYW at 379, score = 9 + 7
umis assigned: [14, 15, 50, 77, 113, 270, 272, 283, 301, 311] and 4 others
of which 14 are surviving nonsolos
reads assigned: 4132
start codons at 16, 37, 81, 391, 470, 531
confident = true

TIG 2[bases=627]
0-22 ==> 38-60 on |373|IGLV4-69|5'UTR| [len=60] (mis=0)
22-381 ==> 0-359 on |374|IGLV4-69|L-REGION+V-REGION| [len=359] (mis=0)
378-416 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
416-627 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 8 umis using 376 reads
cdr3 = CQTWGTGIQVF at 355, score = 8 + 8
umis assigned: [62, 92, 105, 153, 155, 245, 246, 316, 365, 378] and 2 others
of which 12 are surviving nonsolos
reads assigned: 4409
start codons at 22, 183, 223, 239, 338
confident = true

TIG 3[bases=589]
53-413 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=0)
416-453 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
453-589 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 4 umis using 125 reads
cdr3 = CMQGTHWPPGTF at 389, score = 9 + 8
umis assigned: [160, 168, 225, 344]
of which 4 are surviving nonsolos
reads assigned: 984
start codons at 53, 86, 114, 122, 210, 372, 392, 495
confident = true

REJECT CONTIGS

TIG 1[bases=641]
9-33 ==> 5788-5812 on segment before IGLV2-34 exon 1 [len=6000] (mis=0)
28-94 ==> 5809-5875 on segment before IGLV2-34 exon 1 [len=6000] (mis=3)
47-395 ==> 0-348 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
401-430 ==> 9-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
430-641 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [10, 16, 55, 76, 94, 104, 107, 136, 163, 169] and 14 others
of which 24 are surviving nonsolos
reads assigned: 5847
start codons at 3, 47, 204, 248, 255, 258
confident = false
did not find CDR3
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.20 = AGTGGGACACTTACGA-1

using 7166 reads

====================================================================================

graph has 4647 edges initially, 60 edges after simplification

total ucounts = 1035
nonsolo ucounts = 444[2^147, 3^107, 4^57, 5^42, 6^24, 7^15, 8^9, 9^7, 10^7, 11^6, 12, 15^2, 19, 25, 28, 156, 166, 176, 178^2, 266, 298, 312, 315, 324, 325, 327, 337, 366, 380, 391, 393]
surviving nonsolo ucounts = 17[156, 166, 176, 178^2, 266, 298, 312, 315, 324, 325, 327, 337, 366, 380, 391, 393]
ids = [234, 768, 546, 279, 588, 629, 251, 667, 802, 158, ...]

====================================================================================

UMI info for barcode AGTGGGACACTTACGA-1 contig 1 = ACATGGGAAG...
umi AGATACGGAA = 343 reads: +442 validated
umi GACTCTGCAC = 179 reads: +442 validated
umi GCCTGCCTGG = 180 reads: +442 validated
umi TATCCATCAA = 165 reads: +442 validated

UMI info for barcode AGTGGGACACTTACGA-1 contig 2 = GATCAGGACT...
umi ATGTTGTCCA = 324 reads: +397 validated
umi ATTCACTGCG = 365 reads: +397 validated
umi CATACGGATC = 156 reads: +397 validated
umi CATTGTTCAT = 296 reads: +397 validated
umi CCATTAGCCA = 176 reads: +397 validated
umi CCCACGGGCT = 386 reads: +397 validated
umi GCACGTCCAC = 328 reads: +397 validated
umi GCTGCTCCCT = 332 reads: +397 validated
umi GGCCGTCCCT = 267 reads: +397 validated
umi GTCACATCAG = 312 reads: +397 validated
umi TAGAAACACT = 60 reads: -367 +1 -1X +5 -1XX +5 -1XX +1 -1XX +1 -1XX +4 -1XX +5 -1XX +1 invalidated
umi TCACGTATAT = 320 reads: +397 validated
umi TCCCAGTCTA = 384 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=627]
0-25 ==> 6-31 on |185|IGHV4-34|5'UTR| [len=31] (mis=0)
25-396 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=18)
416-467 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
467-627 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 4 umis using 107 reads
cdr3 = CARYGRQQSNSWWFDPW at 385, score = 9 + 7
umis assigned: [85, 546, 588, 768]
of which 4 are surviving nonsolos
reads assigned: 851
start codons at 2, 25, 46, 90, 176, 521
confident = true

TIG 2[bases=563]
0-30 ==> 0-30 on |274|IGKV2D-29|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=24)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 12 umis using 555 reads
cdr3 = CMQNVHLPYTF at 366, score = 9 + 8
umis assigned: [158, 162, 234, 251, 279, 282, 572, 611, 629, 667] and 3 others
of which 13 are surviving nonsolos
reads assigned: 3653
start codons at 30, 63, 99, 250, 349, 369, 376, 469
confident = true
now this is a cell
paired!

GACACGGCTGTGTATTACTGTGCGAGATACGGAAGGCAGCAGTCCAACTCTTGGTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCCGAGTGGAGGCTGAGGATGTTGGACTTTATTACTGCATGCAAAATGTACACCTTCCGTACACTTTTGGCCAGGGGACAAGGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.29 = AGTGGGACAGTCAGCC-1

using 384 reads

====================================================================================

graph has 160 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 5, 371]
surviving nonsolo ucounts = 1[371]
ids = [2]

====================================================================================

UMI info for barcode AGTGGGACAGTCAGCC-1 contig 1 = GATCAGGACT...
umi CCGCAAGCGT = 368 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CMQALQTPLFTF at 366, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 361
start codons at 30, 63, 99, 187, 349, 369, 472
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.36 = AGTGGGACATACGCCG-1

using 412 reads

====================================================================================

graph has 202 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[4^2, 5, 7, 386]
surviving nonsolo ucounts = 1[386]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGACATACGCCG-1 contig 1 = GAGTCAGACC...
umi AAACTACACG = 393 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=549]
0-31 ==> 22-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
31-376 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
385-413 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYYSYPRTF at 352, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 385
start codons at 25, 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.40 = AGTGGGACATCGTCGG-1

using 97 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 9, 82]
surviving nonsolo ucounts = 1[82]
ids = [2]

====================================================================================

UMI info for barcode AGTGGGACATCGTCGG-1 contig 1 = GGAATCAGTC...
umi CCCCTTCTCA = 74 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=474]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-474 ==> 0-60 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 71
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.45 = AGTGGGAGTAAAGTCA-1

using 154 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 3, 4, 5, 133]
surviving nonsolo ucounts = 1[133]
ids = [8]

====================================================================================

UMI info for barcode AGTGGGAGTAAAGTCA-1 contig 1 = AGCCTGGGCC...
umi GAACGTCCGT = 132 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=627]
0-40 ==> 12-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
40-374 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=18)
378-416 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
416-627 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQAWDSTEVVF at 355, score = 6 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 129
start codons at 40, 45, 334, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.47 = AGTGGGAGTAAGAGGA-1

using 262 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 6, 250]
surviving nonsolo ucounts = 1[250]
ids = [3]

====================================================================================

UMI info for barcode AGTGGGAGTAAGAGGA-1 contig 1 = AGTGCTTTCT...
umi TCTTCTTCCT = 245 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=530]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
405-453 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
453-530 ==> 0-77 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 23 reads
cdr3 = CARYFAFVNYYFDKW at 377, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 17, 38, 82
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.48 = AGTGGGAGTAAGGGAA-1

using 515 reads

====================================================================================

graph has 276 edges initially, 52 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[7, 9, 164, 332]
surviving nonsolo ucounts = 2[164, 332]
ids = [3, 0]

====================================================================================

UMI info for barcode AGTGGGAGTAAGGGAA-1 contig 1 = GAATCAGACC...
umi CCCCACATAT = 167 reads: +388 validated

UMI info for barcode AGTGGGAGTAAGGGAA-1 contig 2 = GTCAGTCTCA...
umi AAAGAATCTC = 331 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |245|IGKV1D-16|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |246|IGKV1D-16|L-REGION+V-REGION| [len=351] (mis=0)
376-413 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQQYNSYPLTF at 352, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 163
start codons at 25, 31, 87, 100, 236, 455
confident = false

TIG 2[bases=550]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=0)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQSYSTPPWTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 325
start codons at 23, 29, 85, 98, 234, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.51 = AGTGGGAGTACCGAGA-1

using 339 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2, 5, 6, 7, 312]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=568]
1-81 ==> 5533-5613 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=3)
31-396 ==> 0-365 on |734|IGKV2D-40|L-REGION+V-REGION| [len=365] (mis=3)
394-432 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [5]
of which 0 are surviving nonsolos
reads assigned: 308
start codons at 31, 64, 100, 188, 191, 353, 373, 474
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.53 = AGTGGGAGTACGAAAT-1

using 14094 reads

====================================================================================

graph has 5960 edges initially, 74 edges after simplification

total ucounts = 664
nonsolo ucounts = 335[2^107, 3^60, 4^47, 5^26, 6^14, 7^15, 8^9, 9^3, 11, 12, 15^2, 24, 25, 32, 33, 37, 41, 75, 83, 86, 96, 104, 128, 147, 165, 166, 177, 180, 185, 208, 209, 218, 222, 229, 233, 241, 243, 246, 247, 261, 273, 289, 298, 300, 303, 308, 309, 315^2, 319, 321, 342, 360, 373, 380, 396, 500, 590, 620, 728, 732]
surviving nonsolo ucounts = 46[32, 33, 37, 75, 86, 96, 104, 128, 147, 165, 166, 177, 180, 185, 208, 209, 218, 222, 229, 233, 241, 243, 246, 247, 261, 273, 289, 298, 300, 303, 308, 309, 315^2, 319, 321, 342, 360, 373, 380, 396, 500, 590, 620, 728, 732]
ids = [362, 17, 18, 91, 565, 311, 628, 569, 574, 157, ...]

====================================================================================

UMI info for barcode AGTGGGAGTACGAAAT-1 contig 1 = GAGCTCTGGG...
umi AACGGACCAT = 18 reads: -397X +1 -1X +3 -2X +2 -1 +14 invalidated
umi AAGCCCTGAT = 239 reads: +421 validated
umi ACCTGGTTTA = 220 reads: +417 -4 non-validated
umi ACTCAAATCT = 297 reads: +421 validated
umi ACTCTCCCTT = 249 reads: +421 validated
umi ACTTGAAGCA = 74 reads: +392 -7 +9 -1 +12 non-validated
umi ATCCGCATTA = 210 reads: +421 validated
umi ATTCCTCGTA = 2 reads: -373X +1 -1XX +5 -2XX +1 -2XX +13 -1XX +22 invalidated
umi CGTTACACCA = 97 reads: +389 -1 +11 -20 non-validated
umi CTTCTCGCAT = 31 reads: +283 -22 +59 -1 +20 -2 +1 -1 +1 -1 +25 -5 non-validated
umi GTTTCCTTTG = 156 reads: +421 validated
umi TAACTGCTTT = 291 reads: +421 validated
umi TATGCTACGC = 182 reads: +419 -2 non-validated
umi TCCGACTTCC = 167 reads: +393 -1 +2 -25 non-validated
umi TCTCTCACGC = 83 reads: +401 -1 +4 -1 +2 -1 +1 -1 +9 non-validated
umi TCTGGATACT = 127 reads: +421 validated
umi TGGATGTCCC = 374 reads: +421 validated
umi TTATCGTGTA = 250 reads: +421 validated
umi TTATTTGGTC = 100 reads: +412 -9 non-validated
umi TTCAGTCCTA = 205 reads: +421 validated
umi TTTTTAAGTA = 229 reads: +421 validated

UMI info for barcode AGTGGGAGTACGAAAT-1 contig 2 = GCTCTGCTTC...
umi AACCTTCAGC = 32 reads: -6 +23 -1 +361 non-validated
umi ACCATGAGTC = 262 reads: +391 validated
umi ATCCTAAGGC = 247 reads: +391 validated
umi ATTGCATTGT = 500 reads: +391 validated
umi CACAAACGTG = 187 reads: +391 validated
umi CACAGTAATT = 219 reads: +391 validated
umi CCAGTCGAGC = 245 reads: +391 validated
umi CGTAGAACTG = 288 reads: +391 validated
umi CTTACACCTT = 391 reads: +391 validated
umi TCAACCTTTC = 736 reads: -343X +1 -8X +39 invalidated

GOOD CONTIGS

TIG 1[bases=572]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=0)
438-458 ==> 0-20 on |10|IGHD1-26|D-REGION| [len=20] (mis=2)
453-501 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=0)
501-572 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 12 umis using 196 reads
cdr3 = CARVGPYSGSYYFDYW at 422, score = 9 + 7
umis assigned: [18, 23, 65, 79, 82, 91, 135, 157, 311, 362] and 11 others
of which 21 are surviving nonsolos
reads assigned: 3529
start codons at 80, 231, 236, 289, 294, 297, 315, 383
confident = true

TIG 2[bases=653]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=1)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
442-653 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 9 umis using 360 reads
cdr3 = CQSYDSSLSGWVF at 375, score = 8 + 8
umis assigned: [17, 49, 137, 161, 183, 188, 230, 301, 354, 513]
of which 10 are surviving nonsolos
reads assigned: 3057
start codons at 51, 205, 208, 259, 358, 385
confident = true

REJECT CONTIGS

TIG 1[bases=542]
8-89 ==> 5665-5746 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=3)
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
380-406 ==> 12-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
406-542 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [12, 13, 237, 262, 358, 383, 394, 410, 423, 531] and 2 others
of which 12 are surviving nonsolos
reads assigned: 3906
start codons at 32, 38, 94, 107, 243, 448
confident = false
did not find CDR3
now this is a cell
paired!

GAGGACACGGCTGTGTATTACTGTGCGAGAGTGGGCCCCTATAGTGGGAGCTACTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.56 = AGTGGGAGTAGCGTAG-1

using 9530 reads

====================================================================================

graph has 4170 edges initially, 38 edges after simplification

total ucounts = 643
nonsolo ucounts = 307[2^108, 3^66, 4^39, 5^26, 6^12, 7^7, 8^5, 9^6, 11^2, 13, 14, 15, 16^2, 18, 29, 63, 82, 99, 111, 144, 153, 179, 210, 245, 256, 259, 270, 277, 286, 288, 301, 310^2, 323, 329, 332, 333, 338, 379, 383^2, 403, 528, 562]
surviving nonsolo ucounts = 28[63, 82, 99, 111, 144, 153, 179, 210, 245, 256, 259, 270, 277, 286, 301, 310^2, 323, 329, 332, 333, 338, 379, 383^2, 403, 528, 562]
ids = [407, 233, 57, 586, 126, 603, 156, 150, 247, 377, ...]

====================================================================================

UMI info for barcode AGTGGGAGTAGCGTAG-1 contig 1 = ATCATCCAAC...
umi ACCTCCTCTG = 277 reads: +430 validated
umi AGTACTACCC = 315 reads: +430 validated
umi AGTTGGTTGC = 286 reads: +430 validated
umi ATACAACCCA = 149 reads: +375 -1 +54 non-validated
umi ATGCGCACTT = 191 reads: +335 -1X +94 invalidated
umi ATGGCTTAGG = 366 reads: +430 validated
umi ATGTGTTCCT = 166 reads: +430 validated
umi CCACCGGCAT = 432 reads: -401X +2 -9XX +2 -2XX +1 -2XX +2 -2XX +2 -1XX +4 invalidated
umi GCCATGTAAC = 349 reads: +430 validated
umi GTAGTAGCCG = 63 reads: +393 -37 non-validated
umi TATAAGCTGT = 267 reads: +430 validated
umi TTCCTTTAGT = 157 reads: +430 validated

UMI info for barcode AGTGGGAGTAGCGTAG-1 contig 2 = AGAGCTCTGG...
umi ACCCAGGCCA = 279 reads: +379 validated
umi AGTTCGTGTA = 286 reads: +379 validated
umi ATGCTTATCA = 342 reads: +379 validated
umi CCACTTTTAA = 78 reads: -379 non-validated
umi CCAGATCGAG = 380 reads: +379 validated
umi CCCGGGTATC = 244 reads: +379 validated
umi CTAGTAGCAC = 313 reads: +379 validated
umi GCCTTAATAG = 339 reads: +379 validated
umi GCTCAGGTAC = 304 reads: +379 validated
umi GGAATGCCGC = 254 reads: +379 validated
umi GGTTCCTTCT = 527 reads: +379 validated
umi GTCCTAGTGG = 331 reads: +379 validated
umi GTCTGGAGAC = 257 reads: +6 -1XX +49 -1XX +8 -1XX +3 -1XX +15 -1XX +10 -1XX +56 -1XX +63 -1XX +12 -1XX +7 -1XX +30 -1XX +20 -1XX +3 -1XX +2 -1XX +16 -1XX +8 -20X +4 -1XX +2 -1XX +5 -1XX +9 -1XX +4 -1XX +7 invalidated
umi TCCTTTTCAA = 384 reads: +379 validated
umi TTAAATGGAA = 111 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=559]
0-58 ==> 246-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=0)
58-411 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=4)
408-435 ==> 0-27 on |18|IGHD3-10|D-REGION| [len=27] (mis=5)
438-488 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=0)
488-559 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 8 umis using 198 reads
cdr3 = CAIHYYGSGSKDDDAFDIW at 400, score = 9 + 8
umis assigned: [60, 105, 117, 126, 150, 153, 156, 229, 353, 407] and 2 others
of which 12 are surviving nonsolos
reads assigned: 2968
start codons at 58, 214, 256, 322, 355, 416, 434, 437, 440, 469
confident = true

TIG 2[bases=559]
0-44 ==> 53-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
44-389 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=1)
385-423 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 13 umis using 575 reads
cdr3 = CQQYNNWPTF at 365, score = 9 + 8
umis assigned: [58, 114, 152, 233, 234, 247, 294, 362, 369, 377] and 5 others
of which 14 are surviving nonsolos
reads assigned: 4340
start codons at 44, 113, 249, 465
confident = true
now this is a cell
paired!

GCCGTGTATTACTGTGCAATTCATTACTATGGTTCGGGGAGTAAGGATGATGATGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG <==> ACCATCAGCAGCCTGCAGTCTGAAGATTTTGCAGTTTATTACTGTCAGCAGTATAATAACTGGCCGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.64 = AGTGGGAGTCGCGGTT-1

using 558 reads

====================================================================================

graph has 218 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[555]
surviving nonsolo ucounts = 1[555]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGAGTCGCGGTT-1 contig 1 = TGGGATCAGT...
umi AAGAACGGCG = 563 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 103 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 548
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.77 = AGTGGGAGTGTGAATA-1

using 556 reads

====================================================================================

graph has 308 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[4^2, 232, 315]
surviving nonsolo ucounts = 2[232, 315]
ids = [2, 0]

====================================================================================

UMI info for barcode AGTGGGAGTGTGAATA-1 contig 1 = AGTTAGGACC...
umi AATGATGTCA = 318 reads: +385 validated

UMI info for barcode AGTGGGAGTGTGAATA-1 contig 2 = AAACCACACC...
umi ATCAGTCTGA = 224 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=542]
0-21 ==> 76-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
21-366 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
367-406 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
406-542 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQYNNWPPYTF at 342, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 313
start codons at 21, 90, 226, 448
confident = false

TIG 2[bases=604]
0-48 ==> 11-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
48-401 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
450-484 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
484-604 ==> 0-120 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 390, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 216
start codons at 48, 246, 251, 268, 312, 345, 538
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.85 = AGTGGGAGTTCCATGA-1

using 866 reads

====================================================================================

graph has 302 edges initially, 24 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[269, 590]
surviving nonsolo ucounts = 2[269, 590]
ids = [6, 2]

====================================================================================

UMI info for barcode AGTGGGAGTTCCATGA-1 contig 1 = AGCTTCAGCT...
umi CCTCCGCGGA = 589 reads: +388 validated

UMI info for barcode AGTGGGAGTTCCATGA-1 contig 2 = GGGGTCACAA...
umi TCTGATCGGC = 270 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-355 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 94 reads
cdr3 = CVAWDDSLYAWVF at 368, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 583
start codons at 47, 351, 381, 393, 567
confident = false

TIG 2[bases=634]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=26)
385-423 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
423-634 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 40 reads
cdr3 = CFSYGNTGRVF at 362, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 264
start codons at 38, 249, 372, 555
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.86 = AGTGGGAGTTGGACCC-1

using 267 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 259]
surviving nonsolo ucounts = 1[259]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGAGTTGGACCC-1 contig 1 = ACCCAAAAAC...
umi ACTGCACCCG = 230 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=579]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-579 ==> 0-89 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 228
start codons at 54, 252, 257, 274, 318, 351, 544
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.88 = AGTGGGAGTTGGTGGA-1

using 208 reads

====================================================================================

graph has 128 edges initially, 30 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[4, 100^2]
surviving nonsolo ucounts = 2[100^2]
ids = [2, 4]

====================================================================================

UMI info for barcode AGTGGGAGTTGGTGGA-1 contig 1 = TCCCCTAGAT...
umi CGTCAACGTT = 88 reads: +351 -1X +84 invalidated

UMI info for barcode AGTGGGAGTTGGTGGA-1 contig 2 = GAGAGCATCA...
umi CGAGGGGCCG = 94 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=499]
0-24 ==> 35-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
24-377 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=23)
426-460 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
460-499 ==> 0-39 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 4 reads
cdr3 = CARYKHFRDPYYHYSGALDHW at 366, score = 7 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 87
start codons at 24, 222, 227, 244, 288, 321
confident = false

TIG 2[bases=527]
0-64 ==> 0-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
64-417 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=7)
440-488 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
488-527 ==> 0-39 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 10 reads
cdr3 = CARSLVSYSSSWIFDYW at 406, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 91
start codons at 64, 262, 267, 299, 328, 361
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.101 = AGTGGGATCCAAACTG-1

using 252 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 246]
surviving nonsolo ucounts = 1[246]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGATCCAAACTG-1 contig 1 = GAGTCAGTCT...
umi CTGATTCCCT = 227 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=503]
25-292 ==> 0-267 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=15)
295-381 ==> 267-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-503 ==> 0-87 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQSYSSPWTF at 355, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 25, 31, 87, 100, 232, 236, 458
confident = false
see insertion of GGG at pos 267 on |253|IGKV1D-39|L-REGION+V-REGION|
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.109 = AGTGGGATCCTTGGTC-1

using 59 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 6[3, 6, 7, 12, 13, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.116 = AGTGGGATCGTGGGAA-1

using 173 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[172]
surviving nonsolo ucounts = 1[172]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGATCGTGGGAA-1 contig 1 = AGCTGTGGGT...
umi CGCCTGCCTT = 168 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=568]
0-41 ==> 73-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
41-394 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
391-429 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
429-568 ==> 0-139 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CASWDDSLRGRVF at 362, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 166
start codons at 41, 195, 345, 370, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.118 = AGTGGGATCGTTACGA-1

using 1111 reads

====================================================================================

graph has 356 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2, 3, 4, 1095]
surviving nonsolo ucounts = 1[1095]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.124 = AGTGGGATCTCTGAGA-1

using 294 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 284]
surviving nonsolo ucounts = 1[284]
ids = [5]

====================================================================================

UMI info for barcode AGTGGGATCTCTGAGA-1 contig 1 = GGGAGAGGAG...
umi TGTTATTGTG = 250 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=498]
0-73 ==> 148-221 on |145|IGHV3-53|5'UTR| [len=221] (mis=0)
73-423 ==> 0-350 on |146|IGHV3-53|L-REGION+V-REGION| [len=350] (mis=10)
447-497 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=1)
junction support: 1 umis using 18 reads
cdr3 = CARDILTGPNRGDAFDIW at 412, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 73, 229, 373, 382, 449, 478
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.125 = AGTGGGATCTCTGCTG-1

using 124 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 120]
surviving nonsolo ucounts = 1[120]
ids = [0]

====================================================================================

UMI info for barcode AGTGGGATCTCTGCTG-1 contig 1 = ACTAGAAGTC...
umi CACTGCCACG = 106 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=505]
0-57 ==> 23-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
57-408 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=0)
425-475 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
475-505 ==> 0-30 on |60|IGHM|C-REGION| [len=1358] (mis=1)
junction support: 1 umis using 7 reads
cdr3 = CARESSGWNYAFDIW at 399, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 104
start codons at 57, 208, 213, 266, 271, 274, 292, 360, 427, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.129 = AGTGGGATCTGATTCT-1

using 2151 reads

====================================================================================

graph has 1930 edges initially, 10 edges after simplification

total ucounts = 533
nonsolo ucounts = 216[2^86, 3^50, 4^26, 5^17, 6^10, 7^6, 8^7, 9^4, 10, 12, 14, 15^2, 18, 231, 251, 272, 291]
surviving nonsolo ucounts = 4[231, 251, 272, 291]
ids = [123, 117, 394, 191]

====================================================================================

UMI info for barcode AGTGGGATCTGATTCT-1 contig 1 = AGAGAGGTGC...
umi ATCGAGTGCT = 249 reads: +436 validated
umi ATGCGTCGAC = 234 reads: +436 validated
umi CCGACACCAT = 292 reads: +436 validated

UMI info for barcode AGTGGGATCTGATTCT-1 contig 2 = GAAGACAGGA...
umi TACACACCAC = 264 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=579]
0-72 ==> 7-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
72-425 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=0)
445-508 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=3)
508-579 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 53 reads
cdr3 = CAREIAVAGRPSYYYYGMDVW at 414, score = 9 + 7
umis assigned: [117, 123, 191]
of which 3 are surviving nonsolos
reads assigned: 764
start codons at 72, 223, 228, 375, 465
confident = true

TIG 2[bases=536]
0-28 ==> 86-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
28-381 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
378-416 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
416-536 ==> 0-120 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CAAWDDSLNGYVF at 349, score = 8 + 8
umis assigned: [394]
of which 1 are surviving nonsolos
reads assigned: 259
start codons at 28, 332, 357, 362, 374, 380
confident = true
now this is a cell
paired!

TATTACTGTGCGAGAGAGATAGCAGTGGCTGGTCGTCCTTCTTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> GGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTTATGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.130 = AGTGGGATCTGGCGTG-1

using 207 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3^2, 10, 187]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.138 = AGTGTCAAGAAGAAGC-1

using 195 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[195]
surviving nonsolo ucounts = 1[195]
ids = [0]

====================================================================================

UMI info for barcode AGTGTCAAGAAGAAGC-1 contig 1 = GGGTCAGTCC...
umi CCTACTACTC = 184 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=498]
31-279 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
413-498 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLHYDNRRRTF at 352, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 182
start codons at 31, 87, 100, 239, 362, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.139 = AGTGTCAAGAAGATTC-1

using 232 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^2, 7, 12, 205]
surviving nonsolo ucounts = 1[205]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 14.141 = AGTGTCAAGACTAGGC-1

using 97 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 16[2^4, 3, 4^2, 5, 7^3, 8^2, 9, 10, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.06
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk014-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk014-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

12.728 seconds used processing barcodes, peak mem = 0.23
