[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.35 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk012-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk012-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk012.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.0 = AGGCCGTAGCGTTCCG-1

using 214 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[214]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.7 = AGGCCGTAGGAATCGC-1

using 138 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[134]
surviving nonsolo ucounts = 1[134]
ids = [1]

====================================================================================

UMI info for barcode AGGCCGTAGGAATCGC-1 contig 1 = ATGGACCTCC...
umi AGCTTAGTCC = 123 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=526]
0-371 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=8)
388-436 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
436-526 ==> 0-90 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CARAHGDYYTLLDYW at 360, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 123
start codons at 0, 21, 65, 151, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.10 = AGGCCGTAGGACTGGT-1

using 133 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[133]
surviving nonsolo ucounts = 1[133]
ids = [0]

====================================================================================

UMI info for barcode AGGCCGTAGGACTGGT-1 contig 1 = GAGTGCTTTC...
umi TTTGTTACTC = 126 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=542]
39-176 ==> 0-137 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=25)
176-302 ==> 140-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=30)
423-469 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=12)
469-542 ==> 0-73 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 18 reads
cdr3 = CARGLLVRGILGKGYYLDLW at 378, score = 8 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 125
start codons at 39, 83, 348
confident = false
see deletion of 3 bases at pos 137 on |197|IGHV4/OR15-8|L-REGION+V-REGION|
>vscore_12.10_77.1%
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTCCTGTCGCAGGGACAGCTGCGAGAGTGGGGCGGAGGCCTGTTGGAGACCTCGCAGACGCTGTCACTCACCTGTTCTGTTTACGGTCGAGTCTTCGCTGGCTCCTTCTGGACTTGGATCCGCCAATCCCCAGGCAAGGGGCCGGAGTGGATTGGAGAAATTGCTCAGGGTGGAAGACCCAACTACAACCCGGCCCTCCAGAGTCGCGTCACCATTTCAACTGACACGTCGCAGATCCAGTTCTCCCTGACTGTGAGGTCTATGACCGCCGCGGACTCGGCTACATATTACTGTGCCAGGGG
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.11 = AGGCCGTAGGCAAAGA-1

using 11856 reads

====================================================================================

graph has 5811 edges initially, 48 edges after simplification

total ucounts = 1151
nonsolo ucounts = 472[2^189, 3^100, 4^52, 5^33, 6^21, 7^14, 8^15, 9^3, 10^3, 11^2, 12, 13^3, 14, 16, 31, 52, 73, 107, 125, 129, 152, 174, 222, 231, 249, 260, 263, 294, 298^2, 306, 308, 314, 327^2, 343, 350^2, 351, 356^2, 359, 364, 371, 376, 393, 414, 698]
surviving nonsolo ucounts = 30[125, 129, 152, 174, 222, 231, 249, 260, 263, 294, 298^2, 306, 308, 314, 327^2, 343, 350^2, 351, 356^2, 359, 364, 371, 376, 393, 414, 698]
ids = [158, 938, 225, 606, 320, 278, 353, 110, 421, 99, ...]

====================================================================================

UMI info for barcode AGGCCGTAGGCAAAGA-1 contig 1 = AGAGCTCTGG...
umi ACGGACACTG = 363 reads: +385 validated
umi ACGGCCTATT = 306 reads: +385 validated
umi AGCCTATCTA = 299 reads: +385 validated
umi AGGATTGCCT = 249 reads: +385 validated
umi ATGACGGCTT = 129 reads: +385 validated
umi CACACGTCTG = 414 reads: +385 validated
umi CACTCTACTG = 119 reads: +285 -36 +56 -8 non-validated
umi CAGAACACTC = 366 reads: +385 validated
umi CCCCAGGGGT = 220 reads: +385 validated
umi CGGCGCGCTC = 269 reads: +385 validated
umi CTCGTTCGGT = 345 reads: +385 validated
umi GCACTCAAAG = 173 reads: +385 validated
umi GCGCTCTTCG = 325 reads: +385 validated
umi GGTAATCCAT = 393 reads: +385 validated
umi GTGCCACGCC = 353 reads: +385 validated
umi TAATCATCGC = 307 reads: +385 validated
umi TCACACAAAT = 365 reads: +385 validated
umi TCACCTTGCG = 331 reads: +385 validated
umi TCGGCTTGAG = 317 reads: +385 validated
umi TCTACTCGGG = 358 reads: +385 validated
umi TCTAGGAATT = 130 reads: +385 validated
umi TCTGCAGGAA = 295 reads: +385 validated
umi TTACCGGTAC = 375 reads: +385 validated
umi TTATCAGACT = 356 reads: +385 validated
umi TTCGTGATGC = 348 reads: +385 validated

UMI info for barcode AGGCCGTAGGCAAAGA-1 contig 2 = ACTTTCTGAG...
umi ATTAATCTGC = 340 reads: +427 validated
umi CATTCCTTGG = 233 reads: +427 validated
umi CCGTTTGTGC = 254 reads: +427 validated
umi TCTCCTACTT = 151 reads: -394X +3 -1X +1 -5X +1 -1XX +4 -3XX +2 -4XX +1 -5XX +2 invalidated

GOOD CONTIGS

TIG 1[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
391-429 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 24 umis using 1085 reads
cdr3 = CQQYGSSPWTF at 368, score = 9 + 8
umis assigned: [53, 55, 99, 110, 158, 211, 225, 233, 320, 421] and 15 others
of which 25 are surviving nonsolos
reads assigned: 7384
start codons at 44, 252, 378, 471
confident = true

TIG 2[bases=533]
0-35 ==> 66-101 on |195|IGHV4-61|5'UTR| [len=101] (mis=0)
35-391 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=1)
396-419 ==> 0-23 on |28|IGHD5-12|D-REGION| [len=23] (mis=2)
414-462 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
462-533 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 55 reads
cdr3 = CARVSRGYSGYDHQDYW at 380, score = 9 + 7
umis assigned: [170, 278, 353, 947]
of which 4 are surviving nonsolos
reads assigned: 960
start codons at 35, 79
confident = true

REJECT CONTIGS

TIG 1[bases=414]
0-167 ==> 173-340 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=0)
165-203 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
203-414 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CQAWDSSTVVF at 142, score = 6 + 8
umis assigned: [131]
of which 1 are surviving nonsolos
reads assigned: 689
start codons at 121, 125
confident = false
not full
VJ delta = 21
not full
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGAGTTAGCCGTGGATATAGTGGCTACGACCACCAGGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.15 = AGGCCGTAGTATGACA-1

using 568 reads

====================================================================================

graph has 252 edges initially, 4 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[3, 4, 5, 274, 277]
surviving nonsolo ucounts = 2[274, 277]
ids = [6, 7]

====================================================================================

UMI info for barcode AGGCCGTAGTATGACA-1 contig 1 = AGAGAAGACA...
umi GTTAACACAC = 260 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=577]
0-31 ==> 83-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
31-384 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
381-419 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
419-577 ==> 0-158 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CASWDDSLRGRVF at 352, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 31, 185, 335, 360, 365
confident = false

REJECT CONTIGS

TIG 1[bases=486]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
393-431 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
431-486 ==> 0-55 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 30, 99, 352, 473
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.19 = AGGCCGTAGTGCAAGC-1

using 310 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 301]
surviving nonsolo ucounts = 1[301]
ids = [7]

====================================================================================

UMI info for barcode AGGCCGTAGTGCAAGC-1 contig 1 = GGAGTCAGAC...
umi TGTACATCCA = 274 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=472]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=4)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=4)
411-472 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CQQYNSYSSF at 353, score = 8 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 269
start codons at 26, 32, 88, 101, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.23 = AGGCCGTAGTGTCCCG-1

using 222 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 214]
surviving nonsolo ucounts = 1[214]
ids = [3]

====================================================================================

UMI info for barcode AGGCCGTAGTGTCCCG-1 contig 1 = CTCCTCTAAA...
umi GTCGATTGAT = 166 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=463]
0-38 ==> 20-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
38-391 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=0)
405-453 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
junction support: 1 umis using 17 reads
cdr3 = CARELRWVGGLDYW at 380, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 166
start codons at 38, 189, 236, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.34 = AGGCCGTCACAAGCCC-1

using 149 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[149]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.36 = AGGCCGTCACCGAATT-1

using 281 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 3[2^2, 268]
surviving nonsolo ucounts = 1[268]
ids = [7]

====================================================================================

UMI info for barcode AGGCCGTCACCGAATT-1 contig 1 = GGCTGGGGTC...
umi TATACACGGG = 258 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
42-403 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
392-430 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
430-556 ==> 0-126 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CSSYTSSSTLVF at 366, score = 8 + 9
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 256
start codons at 42, 199, 243, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.40 = AGGCCGTCACGCGAAA-1

using 593 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 4, 583]
surviving nonsolo ucounts = 1[583]
ids = [1]

====================================================================================

UMI info for barcode AGGCCGTCACGCGAAA-1 contig 1 = GGGAGAGGAG...
umi ACACTCTATT = 581 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=597]
0-74 ==> 6-80 on |139|IGHV3-43|5'UTR| [len=80] (mis=3)
74-429 ==> 0-355 on |140|IGHV3-43|L-REGION+V-REGION| [len=355] (mis=27)
443-495 ==> 11-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
495-597 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CAKDRDLARYYGMDVW at 416, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 575
start codons at 74, 219, 225, 230, 309, 377, 452, 513, 574
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.43 = AGGCCGTCACTATCTT-1

using 11003 reads

====================================================================================

graph has 4831 edges initially, 54 edges after simplification

total ucounts = 880
nonsolo ucounts = 351[2^149, 3^66, 4^39, 5^16, 6^9, 7^5, 8^6, 9^3, 10^2, 12^4, 13^3, 14^2, 15, 16, 17, 26^2, 40, 45, 47, 62^2, 66, 67, 90, 97, 125, 134, 135, 140, 171, 195, 224, 235, 237, 240, 245, 256, 262, 265, 274, 275, 277, 280, 281, 284, 297, 300, 306, 315, 316, 318, 327, 329, 332^2, 338, 344, 378]
surviving nonsolo ucounts = 37[26, 47, 66, 67, 90, 97, 134, 135, 140, 195, 224, 235, 237, 240, 245, 256, 262, 265, 274, 275, 277, 280, 281, 284, 297, 300, 306, 315, 316, 318, 327, 329, 332^2, 338, 344, 378]
ids = [805, 440, 797, 247, 385, 460, 839, 561, 105, 698, ...]

====================================================================================

UMI info for barcode AGGCCGTCACTATCTT-1 contig 1 = GGGGAGGAGT...
umi AGCATTCCTG = 314 reads: +394 validated
umi AGTTTGTTGG = 263 reads: +394 validated
umi ATATGTTTGC = 342 reads: +394 validated
umi ATATTTGGGT = 377 reads: +394 validated
umi CAGGTGTATG = 319 reads: +394 validated
umi CATTAGCATT = 63 reads: +55 -5X +1 -1X +2 -1 +3 -1 +308 -17 invalidated
umi CCCACTTACA = 289 reads: +394 validated
umi CCGTTCTTAG = 281 reads: +394 validated
umi CCTTGCAGCG = 279 reads: +394 validated
umi CGTAACCCAC = 328 reads: +394 validated
umi CTCTCATAGC = 321 reads: +394 validated
umi CTTGGCCCGG = 312 reads: +394 validated
umi GAGCTGCGGG = 254 reads: +394 validated
umi GGCACTATTC = 283 reads: +6 -2X +386 invalidated
umi GGTACTGTCC = 225 reads: +394 validated
umi GGTTACAGGT = 136 reads: +394 validated
umi TCAAATGCGC = 241 reads: +394 validated
umi TCCATAGCCC = 239 reads: +394 validated
umi TCGGCCTACC = 300 reads: +394 validated
umi TCGGTTTTTG = 258 reads: +394 validated
umi TCTCATTTAT = 329 reads: +394 validated
umi TTCTCGAGCA = 136 reads: +394 validated
umi TTTCACTTGG = 264 reads: +394 validated

UMI info for barcode AGGCCGTCACTATCTT-1 contig 2 = TGGGGGCTTT...
umi AAAAGAGGCG = 338 reads: +448 validated
umi AGACTAACGT = 140 reads: -2X +446 invalidated
umi AGCGCGCACT = 335 reads: +448 validated
umi CACAGTTTGT = 299 reads: +448 validated
umi CATTAGGGGC = 331 reads: +448 validated
umi CTCAGCGTCT = 89 reads: +448 validated
umi GAACCATTGA = 47 reads: +417 -31 non-validated
umi GACCAATTAG = 231 reads: +448 validated
umi GATAATATAG = 98 reads: +12 -2 +434 non-validated
umi GGAATATGTG = 278 reads: +448 validated
umi TCCCGCCTAT = 197 reads: +19 -1 +428 non-validated
umi TGTATTTCAT = 65 reads: +441 -7 non-validated
umi TTAAACTGTA = 27 reads: -15 +268 -35 +7 -1 +13 -1 +108 non-validated
umi TTCATATGGG = 278 reads: +448 validated

GOOD CONTIGS

TIG 1[bases=561]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=3)
387-425 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
425-561 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 22 umis using 953 reads
cdr3 = CQLYDNLPPLFTF at 358, score = 9 + 8
umis assigned: [110, 142, 154, 158, 227, 247, 266, 297, 319, 343] and 13 others
of which 23 are surviving nonsolos
reads assigned: 6060
start codons at 31, 37, 93, 106, 245, 368, 467
confident = true

TIG 2[bases=538]
19-396 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=11)
419-467 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
467-538 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 12 umis using 218 reads
cdr3 = CARHGRGSQPRGYFDYW at 385, score = 9 + 7
umis assigned: [5, 105, 115, 209, 248, 385, 440, 448, 460, 526] and 4 others
of which 14 are surviving nonsolos
reads assigned: 2717
start codons at 19, 28, 40, 84, 395
confident = true
now this is a cell
paired!

GACACGGCTGTTTATTACTGTGCGAGACATGGGCGTGGGAGCCAGCCAAGGGGGTACTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACTTTATGATAATCTCCCTCCCCTATTCACTTTCGGCCCTGGGACCAAAGTAGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.54 = AGGCCGTCATCTCCCA-1

using 224 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 1[216]
surviving nonsolo ucounts = 1[216]
ids = [8]

====================================================================================

UMI info for barcode AGGCCGTCATCTCCCA-1 contig 1 = GTCAGACCCA...
umi CTGATCTCGT = 200 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=501]
0-23 ==> 24-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
23-374 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=10)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
411-501 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CQQANSFPPTF at 350, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 195
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.70 = AGGCCGTGTATGCTTG-1

using 259 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[255]
surviving nonsolo ucounts = 1[255]
ids = [3]

====================================================================================

UMI info for barcode AGGCCGTGTATGCTTG-1 contig 1 = GGGCCTCAGG...
umi TTGCGCCCCG = 255 reads: +373 validated

GOOD CONTIGS

TIG 1[bases=561]
0-35 ==> 17-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
35-371 ==> 0-336 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=1)
370-408 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
408-561 ==> 0-153 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQAWDSSVVF at 350, score = 6 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 35, 40, 96, 183, 329, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.73 = AGGCCGTGTCCATCCT-1

using 16 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.77 = AGGCCGTGTCTCTCTG-1

using 153 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 145]
surviving nonsolo ucounts = 1[145]
ids = [6]

====================================================================================

UMI info for barcode AGGCCGTGTCTCTCTG-1 contig 1 = TGCTTCAGCT...
umi GCGTCCTTTC = 142 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=501]
0-47 ==> 4-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
47-403 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
403-441 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
441-501 ==> 0-60 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CQSYDSSLSGLYVF at 371, score = 7 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 139
start codons at 47, 201, 204, 255, 354, 381, 405
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.97 = AGGCCGTGTTTGTTTC-1

using 123 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 119]
surviving nonsolo ucounts = 1[119]
ids = [1]

====================================================================================

UMI info for barcode AGGCCGTGTTTGTTTC-1 contig 1 = CAACCACATC...
umi ACGCCGCACG = 1 reads: -293 +10 -1 +3 -2X +2 -2 +1 -1 +1 -1X +2 -1 +1 -1 +2 -2 +18 -3 +1 -85 invalidated
umi ACGCGGCACT = 116 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=482]
0-49 ==> 255-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=0)
49-402 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=30)
432-482 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=5)
junction support: 1 umis using 10 reads
cdr3 = CARRGRGFTGYEVQGAFDIW at 391, score = 10 + 9
umis assigned: [0, 1]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 49, 205, 247, 313, 346
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.109 = AGGCCGTTCACTTCAT-1

using 301 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 3, 4, 60, 227]
surviving nonsolo ucounts = 2[60, 227]
ids = [7, 1]

====================================================================================

UMI info for barcode AGGCCGTTCACTTCAT-1 contig 1 = AGCTCTGAGA...
umi CCACCTTACA = 223 reads: +424 validated
umi GAACGTTCTG = 55 reads: +359 -35 +30 non-validated

GOOD CONTIGS

TIG 1[bases=584]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-584 ==> 0-81 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [1, 7]
of which 2 are surviving nonsolos
reads assigned: 274
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.114 = AGGCCGTTCATAGCAC-1

using 62 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[57]
surviving nonsolo ucounts = 1[57]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.121 = AGGCCGTTCCGAATGT-1

using 445 reads

====================================================================================

graph has 140 edges initially, 18 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[6, 205, 231]
surviving nonsolo ucounts = 2[205, 231]
ids = [5, 4]

====================================================================================

UMI info for barcode AGGCCGTTCCGAATGT-1 contig 1 = AGCTTCAGCT...
umi GTGTTGGGGC = 205 reads: +388 validated

UMI info for barcode AGGCCGTTCCGAATGT-1 contig 2 = GGGGTCACAA...
umi CATTGTCGCC = 233 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 203
start codons at 47, 201, 351, 376, 381
confident = false

TIG 2[bases=643]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0)
394-432 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
432-643 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CCSYAGSSTPHWVF at 362, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 38, 177, 239, 246, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.124 = AGGCCGTTCCTATGTT-1

using 149 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 146]
surviving nonsolo ucounts = 1[146]
ids = [0]

====================================================================================

UMI info for barcode AGGCCGTTCCTATGTT-1 contig 1 = ACCCAAAAAC...
umi ATATCTATTG = 138 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=501]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 137
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.130 = AGGCCGTTCGCTTAGA-1

using 224 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 220]
surviving nonsolo ucounts = 1[220]
ids = [1]

====================================================================================

UMI info for barcode AGGCCGTTCGCTTAGA-1 contig 1 = GGCACAAGAG...
umi TAACGTTATT = 202 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=515]
0-34 ==> 17-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
34-390 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=5)
384-422 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
422-515 ==> 0-93 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQSYDSSLREVF at 358, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 199
start codons at 34, 188, 191, 242, 341, 368
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.132 = AGGCCGTTCGTTGCCT-1

using 1390 reads

====================================================================================

graph has 1370 edges initially, 18 edges after simplification

total ucounts = 555
nonsolo ucounts = 189[2^98, 3^38, 4^24, 5^12, 6^7, 7^3, 8^3, 9, 10, 11, 441]
surviving nonsolo ucounts = 1[441]
ids = [152]

====================================================================================

UMI info for barcode AGGCCGTTCGTTGCCT-1 contig 1 = GGAGGAGTCA...
umi CACACTTTGG = 445 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=553]
0-29 ==> 0-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=1)
29-380 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=28)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 74 reads
cdr3 = CLQDYTYPFSF at 356, score = 9 + 7
umis assigned: [152]
of which 1 are surviving nonsolos
reads assigned: 435
start codons at 29, 35, 91, 104, 186, 189, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.133 = AGGCCGTTCTAACGGT-1

using 207 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[203]
surviving nonsolo ucounts = 1[203]
ids = [0]

====================================================================================

UMI info for barcode AGGCCGTTCTAACGGT-1 contig 1 = GAGTTCTGGG...
umi ACGGCAGTAT = 187 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=538]
0-21 ==> 19-40 on |356|IGLV3-19|5'UTR| [len=40] (mis=0)
21-358 ==> 0-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=0)
365-403 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
403-538 ==> 0-135 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CNSRDSSGNHLVF at 336, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 187
start codons at 21, 140, 169, 220, 319
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.136 = AGGCCGTTCTCAAGTG-1

using 173 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3^2, 5, 156]
surviving nonsolo ucounts = 1[156]
ids = [5]

====================================================================================

UMI info for barcode AGGCCGTTCTCAAGTG-1 contig 1 = AGTCTCAGTC...
umi GATATATGTA = 147 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=472]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=2)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
408-472 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQQSYSTPLTF at 347, score = 9 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 144
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.146 = AGGGAGTAGAAACCTA-1

using 206 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 200]
surviving nonsolo ucounts = 1[200]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.155 = AGGGAGTAGAGTACCG-1

using 537 reads

====================================================================================

graph has 206 edges initially, 18 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 232, 301]
surviving nonsolo ucounts = 2[232, 301]
ids = [0, 3]

====================================================================================

UMI info for barcode AGGGAGTAGAGTACCG-1 contig 1 = GGGGTCTCAG...
umi TCAACACTCC = 300 reads: +388 validated

UMI info for barcode AGGGAGTAGAGTACCG-1 contig 2 = AGCTTCAGCT...
umi AATCTTGGCC = 232 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=637]
0-38 ==> 125-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
38-392 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
388-426 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
426-637 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CSSHAGSTRVLF at 362, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 38, 195, 246, 345, 372
confident = false

TIG 2[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.159 = AGGGAGTAGCACCGTC-1

using 170 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 165]
surviving nonsolo ucounts = 1[165]
ids = [2]

====================================================================================

UMI info for barcode AGGGAGTAGCACCGTC-1 contig 1 = TCTGCTTCAG...
umi TGAGTTGGAT = 156 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=587]
0-49 ==> 2-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
49-405 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
405-443 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
443-587 ==> 0-144 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQSYDSSLSGLYVF at 373, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 155
start codons at 49, 203, 206, 257, 356, 383, 407, 575
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.172 = AGGGAGTAGGATATAC-1

using 57 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^2, 3^3, 5, 36]
surviving nonsolo ucounts = 1[36]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.181 = AGGGAGTAGTGCGATG-1

using 17 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.187 = AGGGAGTCAAAGTGCG-1

using 277 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 271]
surviving nonsolo ucounts = 1[271]
ids = [0]

====================================================================================

UMI info for barcode AGGGAGTCAAAGTGCG-1 contig 1 = GAGCTGCTCA...
umi CGTACATCTT = 270 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=551]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=6)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQQHATSPFTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 266
start codons at 30, 238, 364, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.191 = AGGGAGTCAAGCTGAG-1

using 405 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 398]
surviving nonsolo ucounts = 1[398]
ids = [2]

====================================================================================

UMI info for barcode AGGGAGTCAAGCTGAG-1 contig 1 = AGAGCTCTGG...
umi ATATAGACCC = 375 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=513]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
392-429 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
429-513 ==> 0-84 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQYGSSPFTF at 368, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 370
start codons at 44, 252, 378, 471
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.192 = AGGGAGTCAAGCTGTT-1

using 1527 reads

====================================================================================

graph has 1376 edges initially, 18 edges after simplification

total ucounts = 489
nonsolo ucounts = 173[2^71, 3^32, 4^28, 5^13, 6^18, 7^4, 9^2, 11, 13, 172, 210, 236]
surviving nonsolo ucounts = 2[210, 236]
ids = [80, 199]

====================================================================================

UMI info for barcode AGGGAGTCAAGCTGTT-1 contig 1 = ACCCAAAAAC...
umi AGGGCGTGCT = 203 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=515]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-515 ==> 0-25 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [80]
of which 1 are surviving nonsolos
reads assigned: 199
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.193 = AGGGAGTCAAGGCTCC-1

using 330 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[5, 322]
surviving nonsolo ucounts = 1[322]
ids = [1]

====================================================================================

UMI info for barcode AGGGAGTCAAGGCTCC-1 contig 1 = TGGGGAGGAG...
umi CAACACGGGG = 327 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=9)
383-420 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CQQSYTTLLTF at 359, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 319
start codons at 32, 38, 94, 107, 243, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.203 = AGGGAGTCACTGAAGG-1

using 294 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[289]
surviving nonsolo ucounts = 1[289]
ids = [3]

====================================================================================

UMI info for barcode AGGGAGTCACTGAAGG-1 contig 1 = TGGGGTCTCA...
umi TCACCGCAGC = 275 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=562]
0-39 ==> 124-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
39-393 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
389-427 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
427-562 ==> 0-135 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CSSHAGSTRVLF at 363, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 39, 196, 247, 346, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.205 = AGGGAGTCAGATCGGA-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.213 = AGGGAGTCATCCGCGA-1

using 395 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 86, 305]
surviving nonsolo ucounts = 2[86, 305]
ids = [3, 1]

====================================================================================

UMI info for barcode AGGGAGTCATCCGCGA-1 contig 1 = GGGGATCAGT...
umi CCGGCAGCGA = 277 reads: +388 validated
umi GTAGCAGAAG = 79 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=507]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-507 ==> 0-92 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 57 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [1, 3]
of which 2 are surviving nonsolos
reads assigned: 350
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.222 = AGGGAGTCATTGAGCT-1

using 406 reads

====================================================================================

graph has 242 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[129, 276]
surviving nonsolo ucounts = 2[129, 276]
ids = [2, 1]

====================================================================================

UMI info for barcode AGGGAGTCATTGAGCT-1 contig 1 = GAGAGAGGAG...
umi TGAGCGGAGC = 270 reads: +424 validated
umi TTTTACTCGG = 128 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=608]
0-73 ==> 6-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
450-497 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
497-608 ==> 0-111 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 2 umis using 38 reads
cdr3 = CARDRAATARLGGMDVW at 415, score = 9 + 7
umis assigned: [1, 2]
of which 2 are surviving nonsolos
reads assigned: 388
start codons at 73, 224, 229, 376, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.224 = AGGGAGTGTAAACCTC-1

using 669 reads

====================================================================================

graph has 250 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[7, 111, 548]
surviving nonsolo ucounts = 2[111, 548]
ids = [2, 5]

====================================================================================

UMI info for barcode AGGGAGTGTAAACCTC-1 contig 1 = GATCAGGACT...
umi TTCGGTCGTT = 505 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=520]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=8)
388-427 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
427-520 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 86 reads
cdr3 = CMQTLQTPYTF at 366, score = 9 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 494
start codons at 30, 63, 99, 177, 187, 349, 369, 469
confident = false

REJECT CONTIGS

TIG 1[bases=565]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-79 ==> 0-43 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=0)
82-191 ==> 0-109 on segment before IGLV1-51 exon 2 [len=109] (mis=2)
188-498 ==> 43-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=8) [SHIFT!]
495-533 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
533-565 ==> 0-32 on |306|IGLC2|C-REGION| [len=317] (mis=0)
cdr3 = CGAWDSSLDVGQF at 466, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 106
start codons at 36, 122, 299, 350, 356, 474, 491
confident = false
not full
frameshifted full length stopped transcript of length 565
VJ delta = -87
delta too large
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.231 = AGGGAGTGTATCGCAT-1

using 956 reads

====================================================================================

graph has 370 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[14, 284, 656]
surviving nonsolo ucounts = 2[284, 656]
ids = [1, 4]

====================================================================================

UMI info for barcode AGGGAGTGTATCGCAT-1 contig 1 = AGCTCTCAGA...
umi CCCAATAGTC = 262 reads: +448 validated

GOOD CONTIGS

TIG 1[bases=577]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=9)
443-470 ==> 0-27 on |18|IGHD3-10|D-REGION| [len=27] (mis=4)
466-527 ==> 0-61 on |58|IGHJ6|J-REGION| [len=61] (mis=4)
527-577 ==> 0-50 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARVGRARITMVRGVPNYYYYMDVW at 421, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 79, 235, 356, 382, 451, 484, 545
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.232 = AGGGAGTGTCGGCTCA-1

using 252 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 248]
surviving nonsolo ucounts = 1[248]
ids = [1]

====================================================================================

UMI info for barcode AGGGAGTGTCGGCTCA-1 contig 1 = GGAGTCAGAC...
umi AGATTTTCGT = 226 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=464]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
414-464 ==> 0-50 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQYNTYSWTF at 353, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 26, 32, 88, 101, 237, 240, 258, 333, 376, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.233 = AGGGAGTGTCTAACGT-1

using 10582 reads

====================================================================================

graph has 4984 edges initially, 54 edges after simplification

total ucounts = 389
nonsolo ucounts = 170[2^44, 3^28, 4^25, 5^9, 6^12, 7^5, 8^3, 10, 14, 18, 22, 32, 64, 71, 81, 86, 99, 115, 139, 145, 150, 159, 169, 170^2, 184, 195, 202, 208, 220, 233, 236, 240, 245, 251, 269, 299, 312, 316, 320, 329, 341, 352, 360, 371, 373, 378, 427, 454, 515, 570]
surviving nonsolo ucounts = 33[22, 64, 71, 86, 139, 150, 169, 170^2, 202, 208, 220, 233, 236, 240, 245, 251, 269, 299, 312, 316, 320, 329, 341, 352, 360, 371, 373, 378, 427, 454, 515, 570]
ids = [322, 264, 178, 177, 44, 73, 24, 166, 199, 175, ...]

====================================================================================

UMI info for barcode AGGGAGTGTCTAACGT-1 contig 1 = AGCTCTGGGA...
umi AATCGCGGAG = 171 reads: +424 validated
umi AGTTTGTGCT = 222 reads: +424 validated
umi ATGCACAATA = 249 reads: +424 validated
umi CACGTCCACT = 233 reads: +424 validated
umi CACTTCTTAT = 240 reads: +424 validated
umi CGCACACCTA = 164 reads: +424 validated
umi GATTCGCTCC = 208 reads: +424 validated
umi TTCATGCTTT = 237 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=664]
0-80 ==> 0-80 on |139|IGHV3-43|5'UTR| [len=80] (mis=1)
80-435 ==> 0-355 on |140|IGHV3-43|L-REGION+V-REGION| [len=355] (mis=28)
458-504 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
504-664 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 8 umis using 204 reads
cdr3 = CVKDMSNRDSSGRFGFW at 422, score = 8 + 7
umis assigned: [24, 77, 94, 116, 117, 166, 222, 359]
of which 8 are surviving nonsolos
reads assigned: 1682
start codons at 80, 225, 236, 297, 315, 383, 434, 558
confident = true

REJECT CONTIGS

TIG 1[bases=568]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
20-72 ==> 5709-5761 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=3)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=19)
394-417 ==> 0-23 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQRRDWPMFTF at 368, score = 8 + 7
umis assigned: [13, 39, 44, 60, 66, 73, 114, 130, 175, 177] and 11 others
of which 21 are surviving nonsolos
reads assigned: 6014
start codons at 47, 252, 348, 392, 425, 474
confident = false
not full
VJ delta = 14
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.237 = AGGGAGTGTGGTCTCG-1

using 418 reads

====================================================================================

graph has 596 edges initially, 12 edges after simplification

total ucounts = 230
nonsolo ucounts = 77[2^36, 3^15, 4^8, 5^8, 6^4, 8^3, 9^2, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.238 = AGGGAGTGTGTAATGA-1

using 203 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[200]
surviving nonsolo ucounts = 1[200]
ids = [0]

====================================================================================

UMI info for barcode AGGGAGTGTGTAATGA-1 contig 1 = AGCTCTGAGA...
umi CGTATCCTTC = 202 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=608]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-608 ==> 0-105 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 196
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.244 = AGGGAGTGTTCCCTTG-1

using 310 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[309]
surviving nonsolo ucounts = 1[309]
ids = [1]

====================================================================================

UMI info for barcode AGGGAGTGTTCCCTTG-1 contig 1 = ACAACAGGCA...
umi CAACTTAGTC = 309 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=561]
0-25 ==> 150-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=1)
25-388 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=20)
386-425 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
425-561 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYYGSPRTF at 364, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 306
start codons at 22, 25, 80, 94, 347, 377, 467
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.262 = AGGGAGTTCCTACAGA-1

using 332 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 320]
surviving nonsolo ucounts = 1[320]
ids = [2]

====================================================================================

UMI info for barcode AGGGAGTTCCTACAGA-1 contig 1 = CCTGGGTCAG...
umi CACTCCCTCC = 322 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=573]
0-52 ==> 0-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
52-400 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=7)
398-437 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
437-573 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQQYGSSPGTF at 376, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 316
start codons at 52, 386, 479
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.264 = AGGGAGTTCCTCATTA-1

using 364 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[10, 47, 302]
surviving nonsolo ucounts = 2[47, 302]
ids = [2, 3]

====================================================================================

UMI info for barcode AGGGAGTTCCTCATTA-1 contig 1 = ACCCTGTGCA...
umi CTACCCGGGA = 299 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=560]
0-36 ==> 11-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
36-387 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=23)
386-424 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
424-560 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQASSFPWTF at 363, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 296
start codons at 36, 42, 98, 111, 247, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.272 = AGGGAGTTCGAATCCA-1

using 66 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[66]
surviving nonsolo ucounts = 1[66]
ids = [0]

====================================================================================

UMI info for barcode AGGGAGTTCGAATCCA-1 contig 1 = GGGTGTCTCA...
umi GCGCATTTTC = 62 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=415]
12-349 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=7)
353-391 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
391-415 ==> 0-24 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CQSTDSSGIYVF at 327, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 61
start codons at 12, 73, 142, 160, 355
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.273 = AGGGAGTTCGATCCCT-1

using 739 reads

====================================================================================

graph has 324 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[81, 160, 495]
surviving nonsolo ucounts = 1[495]
ids = [2]

====================================================================================

UMI info for barcode AGGGAGTTCGATCCCT-1 contig 1 = GCACTGGAAG...
umi AGCTCATTAA = 498 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=554]
0-59 ==> 8-67 on |127|IGHV3-33|5'UTR| [len=67] (mis=2)
59-412 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=23)
430-483 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=7)
483-554 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CVRSSWSEQMLWYFNPW at 401, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 487
start codons at 59, 215, 362, 415, 428, 433
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.277 = AGGGAGTTCTCAACTT-1

using 134 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 125]
surviving nonsolo ucounts = 1[125]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=473]
0-63 ==> 5937-6000 on segment before IGKV2D-40 exon 1 [len=6000] (mis=2)
23-69 ==> 3411-3457 on segment before IGKV2OR2-2 exon 1 [len=3837] (mis=2)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=2)
391-429 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
429-473 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 114
start codons at 30, 63, 99, 187, 250, 349, 369
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.278 = AGGGAGTTCTCACATT-1

using 244 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2^2, 240]
surviving nonsolo ucounts = 1[240]
ids = [0]

====================================================================================

UMI info for barcode AGGGAGTTCTCACATT-1 contig 1 = GCTCTGCTTC...
umi CGTTACGCGC = 230 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=481]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-481 ==> 0-36 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.296 = AGGGATGAGAGTGACC-1

using 360 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2^2, 352]
surviving nonsolo ucounts = 1[352]
ids = [2]

====================================================================================

UMI info for barcode AGGGATGAGAGTGACC-1 contig 1 = ATCAGTCCCA...
umi CAATACCCTT = 354 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 350
start codons at 23, 29, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.318 = AGGGATGAGGCGTACA-1

using 447 reads

====================================================================================

graph has 689 edges initially, 4 edges after simplification

total ucounts = 214
nonsolo ucounts = 87[2^31, 3^16, 4^19, 5^10, 6^5, 7^2, 8, 9, 10, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.328 = AGGGATGAGTATGACA-1

using 423 reads

====================================================================================

graph has 204 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 5[2^2, 3, 158, 249]
surviving nonsolo ucounts = 2[158, 249]
ids = [8, 9]

====================================================================================

REJECT CONTIGS

TIG 1[bases=493]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
0-58 ==> 7988-8046 on rc of segment before IGHV2-70 exon 2 [len=8046] (mis=0)
7-76 ==> 10071-10140 on rc of segment before IGHV1-18 exon 2 [len=10140] (mis=6)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=19)
444-493 ==> 0-49 on |53|IGHJ3|J-REGION| [len=50] (mis=6)
cdr3 = CARGGWRLDSSGYQFGALHIW at 400, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 58, 209, 256, 355, 475
confident = false
VJ delta = 10
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.331 = AGGGATGAGTCCCACG-1

using 84 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 13[2^3, 3, 4^2, 5, 6, 8, 9^3, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.332 = AGGGATGAGTCTCGGC-1

using 507 reads

====================================================================================

graph has 232 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^3, 22, 477]
surviving nonsolo ucounts = 2[22, 477]
ids = [2, 6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=443]
3-194 ==> 165-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=6)
194-232 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
232-443 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 162, score = 7 + 8
umis assigned: [2, 6]
of which 2 are surviving nonsolos
reads assigned: 491
start codons at 46, 145, 172, 196, 364
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.333 = AGGGATGAGTGTGGCA-1

using 282 reads

====================================================================================

graph has 136 edges initially, 10 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3^2, 4, 8, 262]
surviving nonsolo ucounts = 2[3, 262]
ids = [5, 2]

====================================================================================

UMI info for barcode AGGGATGAGTGTGGCA-1 contig 1 = AGACCCAGTC...
umi GTCGCAACTG = 234 reads: +388 validated
umi TTTCGATCAC = 4 reads: -89 +3 -1X +3 -1XX +37 -1XX +5 -1XX +6 -1X +1 -76 +16 -1 +12 -1X +16 -1 +6 -1 +1 -108 invalidated

GOOD CONTIGS

TIG 1[bases=491]
0-20 ==> 27-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
20-371 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=1)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
408-491 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CQQANSFPLTF at 347, score = 9 + 9
umis assigned: [2, 5]
of which 2 are surviving nonsolos
reads assigned: 235
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.339 = AGGGATGCAATAAGCA-1

using 15125 reads

====================================================================================

graph has 6651 edges initially, 88 edges after simplification

total ucounts = 735
nonsolo ucounts = 374[2^127, 3^77, 4^40, 5^21, 6^19, 7^20, 8^10, 9^4, 10, 11, 12, 13^2, 14^2, 15, 19, 30, 33, 35, 132, 133, 148, 160, 170, 175, 201, 207, 212, 235, 245, 246, 247, 251, 257, 261, 269^2, 274, 278, 280, 281, 282, 284, 287, 289, 295, 298, 306, 311, 313, 315, 316, 317, 328, 347, 374, 388, 390, 413, 540, 605, 728, 767]
surviving nonsolo ucounts = 44[30, 132, 133, 148, 160, 170, 175, 207, 212, 235, 245, 246, 247, 251, 257, 261, 269^2, 274, 278, 280, 281, 282, 284, 287, 289, 295, 298, 306, 311, 313, 315, 316, 317, 328, 347, 374, 388, 390, 413, 540, 605, 728, 767]
ids = [227, 6, 325, 328, 656, 117, 28, 662, 715, 728, ...]

====================================================================================

UMI info for barcode AGGGATGCAATAAGCA-1 contig 1 = ACTTTCTGAG...
umi CGGTATATTC = 281 reads: +421 validated
umi CTGGAAAACT = 267 reads: +421 validated
umi GATGATTGGC = 337 reads: +421 validated
umi TGCCCCCGAG = 196 reads: -201X +220 invalidated
umi TTAACCCGCT = 163 reads: +421 validated
umi TTGCCACCCT = 397 reads: +421 validated
umi TTTCGGTTTG = 316 reads: +421 validated
umi TTTGTGTCCT = 239 reads: +421 validated

UMI info for barcode AGGGATGCAATAAGCA-1 contig 2 = GCTCTGCTTC...
umi AAATCACCCC = 241 reads: +388 validated
umi ACTTCATTCG = 181 reads: +388 validated
umi CAGTGTAGTG = 266 reads: +388 validated
umi CCAAGTAAGC = 273 reads: +388 validated
umi CCAATTTCTT = 165 reads: +388 validated
umi CCAGGAGATC = 283 reads: +388 validated
umi CGTATATCCG = 272 reads: +388 validated
umi CGTTACCCTT = 242 reads: +388 validated
umi GAGCGCTCGT = 134 reads: +388 validated
umi GCATGGCGAG = 293 reads: +388 validated
umi GGACATCGCA = 353 reads: +388 validated
umi TATTCATAGG = 264 reads: +388 validated
umi TCCCTTAAGA = 600 reads: +388 validated
umi TCTCACGGTC = 260 reads: +388 validated
umi TGATGGAATA = 328 reads: +388 validated
umi TTATCGTGGT = 276 reads: +388 validated
umi TTCAAGTGGG = 307 reads: +388 validated
umi TTTCCTATGT = 211 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=527]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=1)
385-404 ==> 0-19 on |18|IGHD3-10|D-REGION| [len=27] (mis=0)
405-456 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=4)
456-527 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 8 umis using 330 reads
cdr3 = CARGYYYGSGSSWFDPW at 374, score = 9 + 7
umis assigned: [229, 283, 337, 624, 656, 699, 720, 728]
of which 8 are surviving nonsolos
reads assigned: 2166
start codons at 35, 79, 393
confident = true

TIG 2[bases=650]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=4)
401-439 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
439-650 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 18 umis using 740 reads
cdr3 = CQSYDSSLSVVF at 375, score = 8 + 8
umis assigned: [2, 28, 92, 114, 117, 128, 232, 237, 325, 350] and 8 others
of which 18 are surviving nonsolos
reads assigned: 4858
start codons at 51, 205, 208, 259, 358, 385
confident = true

REJECT CONTIGS

TIG 1[bases=715]
0-114 ==> 0-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
114-467 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=0)
466-504 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
504-715 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
umis assigned: [138, 288, 310, 328, 631, 662, 673, 697]
of which 8 are surviving nonsolos
reads assigned: 2693
start codons at 9, 13, 36, 114, 418, 443, 448, 460
confident = false
did not find CDR3

TIG 2[bases=564]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-44 ==> 5956-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
390-428 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=2)
428-564 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [6, 52, 141, 220, 225, 239, 550, 591, 618]
of which 9 are surviving nonsolos
reads assigned: 2703
start codons at 44, 252, 378, 470
confident = false
did not find CDR3
now this is a cell
paired!

GACACGGCCGTGTATTACTGTGCGAGAGGGTATTACTATGGTTCGGGGTCAAGTTGGTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ACTGGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.343 = AGGGATGCAATCCGAT-1

using 164 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 19, 139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.351 = AGGGATGCACCCATTC-1

using 432 reads

====================================================================================

graph has 599 edges initially, 4 edges after simplification

total ucounts = 254
nonsolo ucounts = 68[2^31, 3^15, 4^7, 5^6, 6, 7^2, 8^2, 9, 10^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.353 = AGGGATGCACGAAACG-1

using 129 reads

====================================================================================

graph has 56 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[4, 19, 104]
surviving nonsolo ucounts = 2[19, 104]
ids = [1, 3]

====================================================================================

UMI info for barcode AGGGATGCACGAAACG-1 contig 1 = AAGGAAGCAG...
umi TGTACAGCCA = 95 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=522]
0-29 ==> 222-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
29-361 ==> 0-332 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=36)
373-411 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
411-522 ==> 0-111 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CQVWDRSRDHVVF at 344, score = 6 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 94
start codons at 29, 78, 90, 113, 327, 372
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.357 = AGGGATGCACGGTGTC-1

using 345 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[3, 4, 335]
surviving nonsolo ucounts = 1[335]
ids = [1]

====================================================================================

UMI info for barcode AGGGATGCACGGTGTC-1 contig 1 = GAAGAGCTGC...
umi ACGAGAAGGG = 343 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=554]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYGSSPVTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 334
start codons at 33, 241, 244, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.366 = AGGGATGCAGGGATTG-1

using 694 reads

====================================================================================

graph has 940 edges initially, 8 edges after simplification

total ucounts = 298
nonsolo ucounts = 136[2^57, 3^33, 4^18, 5^10, 6^6, 7^2, 8^3, 9, 10, 11, 16, 17, 19, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.370 = AGGGATGCATACCATG-1

using 309 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 302]
surviving nonsolo ucounts = 1[302]
ids = [4]

====================================================================================

UMI info for barcode AGGGATGCATACCATG-1 contig 1 = GATCAGGACT...
umi TTGTCCAGTT = 283 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=513]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=1)
389-427 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
427-513 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CMQALQTPWTF at 366, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 274
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.385 = AGGGATGGTAAACACA-1

using 42116 reads

====================================================================================

graph has 17641 edges initially, 108 edges after simplification

total ucounts = 2231
nonsolo ucounts = 1108[2^375, 3^223, 4^129, 5^87, 6^54, 7^34, 8^22, 9^11, 10^7, 11^3, 12^7, 14^3, 16, 17, 18^2, 25, 32, 44, 46, 55, 56, 57, 59, 61, 66, 67, 91, 100, 102, 109, 111, 112, 113, 115^2, 117, 119, 135, 138^2, 140, 142, 143, 153, 154, 160, 165, 166, 168, 169, 170^2, 179, 181^2, 183, 184^2, 186, 187^2, 193, 194, 195^2, 198^2, 200, 204, 206, 207^2, 210, 212, 215^2, 216, 218, 221, 223, 225, 227, 230, 236, 237, 240^2, 241^2, 242, 245, 250, 251, 254, 259, 262, 263, 265, 267^2, 273^2, 274, 277, 280, 287, 289, 290, 291^2, 292, 293^2, 295, 298, 299^3, 300, 305, 306^2, 307, 309, 315, 316, 318, 320^2, 321^2, 323, 324^2, 327^2, 329, 331, 333^2, 339, 340, 341^2, 343, 347, 354, 361^2, 369, 374, 376, 384, 386, 388, 390, 400, 424, 435, 504, 583, 593, 964, 979]
surviving nonsolo ucounts = 141[2, 59, 61, 67, 91, 100, 102, 109, 112, 113, 115^2, 117, 119, 135, 138^2, 140, 142, 143, 153, 154, 160, 165, 166, 168, 169, 170^2, 179, 181^2, 183, 184^2, 186, 187^2, 193, 194, 195^2, 198^2, 200, 204, 206, 207^2, 210, 212, 215^2, 216, 218, 221, 223, 225, 227, 230, 236, 237, 240^2, 241^2, 242, 245, 250, 251, 254, 259, 262, 263, 265, 267^2, 273^2, 274, 277, 280, 287, 289, 290, 291^2, 292, 293^2, 295, 298, 299^3, 300, 305, 306^2, 307, 309, 315, 316, 318, 320^2, 321^2, 323, 324^2, 327^2, 329, 331, 333^2, 339, 340, 341^2, 343, 347, 354, 361^2, 369, 374, 376, 384, 386, 388, 390, 400, 424, 435, 504, 583, 593, 964, 979]
ids = [582, 1074, 918, 1609, 1678, 1478, 738, 533, 167, 1746, ...]

====================================================================================

UMI info for barcode AGGGATGGTAAACACA-1 contig 1 = AGCCCTCAGA...
umi AAAGGGATAC = 221 reads: +430 validated
umi ACAATCTTCG = 212 reads: +430 validated
umi ACACTTTTCG = 113 reads: +430 validated
umi ACATACGGCC = 206 reads: +430 validated
umi ATAGGTATCA = 114 reads: +430 validated
umi ATATATCCTT = 205 reads: +430 validated
umi ATCCCTTACC = 161 reads: +430 validated
umi ATTACTTTGC = 110 reads: +430 validated
umi CAACCATTGA = 245 reads: +430 validated
umi CAATACCGAG = 140 reads: +430 validated
umi CACGGTGCCG = 187 reads: +430 validated
umi CACTATTTGC = 140 reads: +423 -7 non-validated
umi CACTGTACCT = 225 reads: +430 validated
umi CAGCCCATAC = 288 reads: +430 validated
umi CATAGAATCC = 302 reads: +430 validated
umi CATTAAACGA = 98 reads: -403X +1 -1X +1 -3X +1 -7X +1 -1X +1 -1X +1 -3X +1 -2X +2 invalidated
umi CCGTACAGCC = 187 reads: +430 validated
umi CCTCCTTACT = 62 reads: +261 -15 +154 non-validated
umi CCTGGCAGGA = 240 reads: +430 validated
umi CGCCATGTCG = 920 reads: +1 -1XX +6 -1XX +6 -1XX +1 -1XX +6 -374X +1 -1XX +1 -3XX +2 -5XX +2 -1XX +1 -3XX +2 -1XX +1 -1XX +1 -4XX +2 invalidated
umi CGCCGAGCCT = 171 reads: +409 -1 +20 non-validated
umi CGCGCTCTCC = 177 reads: +430 validated
umi CGGAAAGCCT = 186 reads: +430 validated
umi CGGAACTCCT = 216 reads: +430 validated
umi CGGTGTGGGC = 198 reads: +430 validated
umi CGTCTTTCTT = 60 reads: +326 -1 +1 -1 +58 -4X +1 -2 +2 -1 +3 -1X +1 -1X +3 -1 +1 -1 +3 -9 +9 invalidated
umi CTAACTCCGG = 198 reads: +430 validated
umi CTACTGCCTC = 573 reads: +1 -1XX +6 -1XX +6 -1X +1 -386X +1 -1XX +1 -3XX +1 -7XX +1 -1XX +1 -1XX +1 -3XX +1 -2XX +2 invalidated
umi CTATTGCCCT = 194 reads: +430 validated
umi CTCACGACTG = 113 reads: +430 validated
umi CTTTATTCTT = 180 reads: +424 -1 +5 non-validated
umi GACTATATCG = 188 reads: +430 validated
umi GACTTTCCAG = 194 reads: +430 validated
umi GATTGGTCAA = 214 reads: +430 validated
umi GCCCACGCGT = 247 reads: +430 validated
umi GCTACGTCAG = 184 reads: +430 validated
umi GCTCAACCCT = 242 reads: +430 validated
umi GCTCACCATG = 623 reads: +175 -3XX +1 -3XX +1 -7XX +1 -7XX +1 -183X +1 -2XX +1 -5XX +1 -1XX +1 -6XX +30 invalidated
umi GGCAGAAAGG = 96 reads: +430 validated
umi TAACCTAACA = 238 reads: +430 validated
umi TAATATGCCG = 121 reads: +430 validated
umi TACCCGGCCT = 87 reads: +429 -1 non-validated
umi TCAACATAAG = 113 reads: +403 -10 +17 non-validated
umi TCCACATGGC = 223 reads: +430 validated
umi TCCCAGTATG = 246 reads: +430 validated
umi TCCCGGGCCT = 121 reads: +430 validated
umi TCTTTCGTAC = 206 reads: +430 validated
umi TGCGCGGTCC = 300 reads: +36 -1XX +393 invalidated
umi TGCTTGTCTG = 149 reads: +430 validated
umi TGTATCAAGC = 169 reads: +430 validated
umi TTAAAAAGGA = 231 reads: +430 validated
umi TTCTGAACGC = 239 reads: +430 validated
umi TTGCACGAAC = 198 reads: +430 validated
umi TTGCTTTCAG = 191 reads: +430 validated
umi TTGTTCAGAT = 169 reads: +430 validated
umi TTTCCGCCTC = 236 reads: +430 validated

UMI info for barcode AGGGATGGTAAACACA-1 contig 2 = TGGGGAGGAG...
umi AAACTTTCAT = 140 reads: +382 validated
umi AACGACGGCA = 320 reads: +382 validated
umi AACGGTACGA = 255 reads: +382 validated
umi AAGTTTAAGT = 323 reads: +382 validated
umi AATCTTCGCT = 256 reads: +382 validated
umi AATTGCCTGC = 362 reads: +382 validated
umi AATTGGCACT = 257 reads: +382 validated
umi ACAATCGTCA = 306 reads: +382 validated
umi ACCGGCGCCT = 207 reads: +382 validated
umi AGAGCACTCA = 311 reads: +382 validated
umi AGGCCCCTTT = 390 reads: +382 validated
umi AGGGTACATC = 355 reads: +382 validated
umi AGTCGAAATC = 343 reads: +382 validated
umi ATAGGCTTGA = 314 reads: +382 validated
umi ATATGAAATG = 368 reads: +382 validated
umi ATCATACGAA = 436 reads: +382 validated
umi ATTACTAGGC = 257 reads: +382 validated
umi ATTAGTACCC = 293 reads: +382 validated
umi ATTATCGTAC = 202 reads: +382 validated
umi ATTCCACTTT = 276 reads: +382 validated
umi CAAATTACTC = 358 reads: +382 validated
umi CAACTTCGAA = 218 reads: +382 validated
umi CACGACTCCT = 290 reads: +382 validated
umi CAGTCATTAT = 538 reads: +133 -1XX +1 -3XX +2 -6XX +1 -7XX +1 -2XX +1 -39X +1 -10X +1 -6XX +1 -1XX +2 -7XX +156 invalidated
umi CAGTCTGTTA = 349 reads: +382 validated
umi CATCCTTCGG = 296 reads: +382 validated
umi CATTATCCAT = 361 reads: +382 validated
umi CCATTCTCTA = 393 reads: +382 validated
umi CCATTGGCCA = 315 reads: +382 validated
umi CCCCGCCAGT = 155 reads: +382 validated
umi CCCCGCCGGA = 140 reads: +382 validated
umi CCCCGTAGCA = 316 reads: +382 validated
umi CCCTATGCGT = 339 reads: +382 validated
umi CCCTTCATGG = 312 reads: +382 validated
umi CCGACTTTCT = 194 reads: +382 validated
umi CCGGCCCTCT = 138 reads: +382 validated
umi CCGTAGTATG = 317 reads: +382 validated
umi CCTCACCCTT = 342 reads: +382 validated
umi CCTCACGGGG = 310 reads: +382 validated
umi CCTTACCTTA = 327 reads: +382 validated
umi CGATTGTCCT = 251 reads: +382 validated
umi CGCATCTAGG = 334 reads: +382 validated
umi CGTCACATGC = 317 reads: +382 validated
umi CGTCACCGAG = 426 reads: -10 +372 non-validated
umi CGTCGGGTAT = 251 reads: +382 validated
umi CTGCGCTTAT = 332 reads: +382 validated
umi CTGTACATTC = 226 reads: +382 validated
umi GACCATCCCA = 341 reads: +382 validated
umi GACCCATGCG = 279 reads: +382 validated
umi GAGATTCTCC = 182 reads: +382 validated
umi GAGTATGTCA = 197 reads: +382 validated
umi GATTTTCCGG = 331 reads: +382 validated
umi GCCCTTCTTT = 322 reads: +382 validated
umi GCTTGCTCTC = 268 reads: +382 validated
umi GGATCTCGAC = 380 reads: +382 validated
umi GGATTACGTA = 214 reads: +382 validated
umi GGGCTTGTGC = 161 reads: +382 validated
umi GGTTCCCGTC = 322 reads: +382 validated
umi GTAATGCGCG = 309 reads: +382 validated
umi GTCCTATTAC = 273 reads: +382 validated
umi GTGACTATCT = 296 reads: +382 validated
umi TAACTCCGGT = 297 reads: +382 validated
umi TAGATCCGCG = 301 reads: +382 validated
umi TATCGGCCCG = 392 reads: +382 validated
umi TCAGTCTCTG = 308 reads: +382 validated
umi TCATTTAGCT = 378 reads: +382 validated
umi TCCAACTCAG = 275 reads: +382 validated
umi TCCATCATCG = 390 reads: +382 validated
umi TCCCTTACGC = 278 reads: +382 validated
umi TCGCAAACGG = 965 reads: +382 validated
umi TCGGGGTGCG = 297 reads: +382 validated
umi TGATCACTTC = 275 reads: +382 validated
umi TGTCTCACTA = 282 reads: +382 validated
umi TTCACCCATC = 385 reads: +382 validated
umi TTCGCCTTCA = 268 reads: +382 validated
umi TTGGATTTCG = 285 reads: +382 validated
umi TTGGGCTGCG = 298 reads: +382 validated
umi TTTGGGCTGT = 168 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=669]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=2)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=36)
463-509 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=6)
509-669 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 48 umis using 859 reads
cdr3 = CARESYVGLYSSTSYPDYW at 421, score = 8 + 7
umis assigned: [30, 157, 167, 174, 421, 424, 465, 533, 576, 599] and 46 others
of which 56 are surviving nonsolos
reads assigned: 11631
start codons at 79, 228, 235, 314, 356, 382, 437, 563
confident = true

TIG 2[bases=556]
38-286 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=33)
382-420 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=4)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 78 umis using 3893 reads
cdr3 = CLHYDNRRRTF at 359, score = 9 + 8
umis assigned: [22, 66, 69, 104, 125, 142, 143, 156, 223, 323] and 68 others
of which 78 are surviving nonsolos
reads assigned: 23307
start codons at 38, 94, 107, 246, 369, 462
confident = true
now this is a cell
paired!

GCTGTCTATTACTGTGCGAGAGAATCTTATGTCGGGCTCTATTCTTCAACCAGTTATCCCGACTACTGGGGCCAGGGAACCCTGGTCACTGTCTCCCCAG <==> GTCTCCAGCCTGCAACCTGAAGATTTTGCCACCTATTATTGTCTACACTATGATAATCGCCGTCGCACCTTCGGCCAAGGGACACGACTGGACATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.387 = AGGGATGGTACAGACG-1

using 337 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 3, 326]
surviving nonsolo ucounts = 1[326]
ids = [8]

====================================================================================

UMI info for barcode AGGGATGGTACAGACG-1 contig 1 = GTCAGTCCCA...
umi TTCTTTATGT = 299 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=506]
0-23 ==> 8-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
23-374 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=17)
373-411 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=3)
411-506 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CQQYDNLPVTF at 350, score = 9 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 23, 29, 85, 98, 237, 360, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.392 = AGGGATGGTAGCGTGA-1

using 995 reads

====================================================================================

graph has 1188 edges initially, 8 edges after simplification

total ucounts = 450
nonsolo ucounts = 193[2^80, 3^33, 4^29, 5^19, 6^6, 7^10, 8^4, 9^5, 10^3, 11, 14^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.395 = AGGGATGGTCACTTCC-1

using 172 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2^3, 9, 153]
surviving nonsolo ucounts = 1[153]
ids = [1]

====================================================================================

UMI info for barcode AGGGATGGTCACTTCC-1 contig 1 = AGTTCACCTT...
umi ATGCCGTCCG = 147 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=467]
16-376 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=2)
371-410 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
410-467 ==> 0-57 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CMQGTHWSTF at 352, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 144
start codons at 16, 49, 77, 85, 173, 335, 355, 452
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.398 = AGGGATGGTCCGAGTC-1

using 110 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[110]
surviving nonsolo ucounts = 1[110]
ids = [0]

====================================================================================

UMI info for barcode AGGGATGGTCCGAGTC-1 contig 1 = AGGAATCAGT...
umi CATTACTGGC = 97 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=425]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
377-415 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CLQHKSYPFTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 96
start codons at 27, 33, 102, 238
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.401 = AGGGATGGTCGAGTTT-1

using 356 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 3, 348]
surviving nonsolo ucounts = 1[348]
ids = [1]

====================================================================================

UMI info for barcode AGGGATGGTCGAGTTT-1 contig 1 = GAAGAGCTGC...
umi ACACCGCGTG = 323 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=515]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=26)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
421-515 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CQQYGSSPMYTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 33, 82, 241, 340, 381, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.414 = AGGGATGGTGCTAGCC-1

using 615 reads

====================================================================================

graph has 238 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 244, 368]
surviving nonsolo ucounts = 2[244, 368]
ids = [1, 2]

====================================================================================

UMI info for barcode AGGGATGGTGCTAGCC-1 contig 1 = ATCACATAAC...
umi CATATCCCGA = 194 reads: +415 validated

UMI info for barcode AGGGATGGTGCTAGCC-1 contig 2 = AGTCTCAGTC...
umi TGCCCGTTAT = 335 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=497]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=3)
425-473 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
473-497 ==> 0-24 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CASTQLWSYFFDYW at 400, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 191
start codons at 58, 209, 256, 355, 417
confident = false

TIG 2[bases=491]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=18)
366-405 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
405-491 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CQQSYSTPSF at 347, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 20, 26, 82, 95, 231, 447
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.419 = AGGGATGGTGTGGTTT-1

using 1957 reads

====================================================================================

graph has 2645 edges initially, 16 edges after simplification

total ucounts = 773
nonsolo ucounts = 400[2^143, 3^93, 4^57, 5^35, 6^27, 7^11, 8^10, 9^8, 10^5, 11, 12^2, 13, 14, 15, 16^3, 23, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.435 = AGGGATGTCAACGGCC-1

using 6245 reads

====================================================================================

graph has 2983 edges initially, 40 edges after simplification

total ucounts = 415
nonsolo ucounts = 189[2^69, 3^34, 4^16, 5^18, 6^8, 7^8, 8^7, 9, 10, 11^2, 13^2, 18, 49, 51, 75, 80, 113, 135, 215^2, 222, 246, 263, 269^2, 270, 276, 313, 331, 337, 361, 420, 427, 443]
surviving nonsolo ucounts = 21[13^2, 49, 80, 113, 135, 215^2, 246, 263, 269^2, 270, 276, 313, 331, 337, 361, 420, 427, 443]
ids = [249, 255, 337, 301, 253, 93, 88, 384, 144, 79, ...]

====================================================================================

UMI info for barcode AGGGATGTCAACGGCC-1 contig 1 = ATACTTTCTG...
umi AGGCCTACAG = 247 reads: +463 validated
umi AGTGCCCATC = 216 reads: +455 -8 non-validated
umi ATAATTTCTA = 133 reads: +461 -2 non-validated
umi ATATGCCCTG = 436 reads: -7X +456 invalidated
umi ATTCCACCCA = 311 reads: +463 validated
umi CAGCTGTCCT = 229 reads: +463 validated
umi TAAACGCTTC = 74 reads: +462 -1 non-validated
umi TTACTGTCCA = 215 reads: +463 validated

UMI info for barcode AGGGATGTCAACGGCC-1 contig 2 = GTCAGAGCCC...
umi AATAAACAGG = 335 reads: +385 validated
umi AATTTTGTTT = 279 reads: +385 validated
umi ACGTCCAGTT = 432 reads: +385 validated
umi AGGCTAACCG = 270 reads: +385 validated
umi ATCGATTCAT = 420 reads: +385 validated
umi CTGGCGCTCC = 362 reads: +385 validated
umi GCGGTTACTT = 13 reads: +275 -45 +42 -1 +13 -9 non-validated
umi GCGTTTACTT = 112 reads: +385 validated
umi GCGTTTTCTT = 13 reads: -19 +2 -1 +3 -1 +9 -1 +68 -1 +109 -1 +1 -1 +3 -1 +121 -27 +1 -1X +11 -2 +1 invalidated
umi GTCGTTTACT = 276 reads: +385 validated
umi TCTTGTCGGG = 26 reads: -346X +1 -1X +1 -2X +2 -1XX +2 -1XX +4 -2XX +8 -1XX +5 -1XX +7 invalidated

GOOD CONTIGS

TIG 1[bases=571]
0-37 ==> 27-64 on |191|IGHV4-4|5'UTR| [len=64] (mis=2)
37-390 ==> 0-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=1)
399-430 ==> 0-31 on |14|IGHD2-2|D-REGION| [len=31] (mis=2)
437-500 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=3)
500-571 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 6 umis using 84 reads
cdr3 = CARGGPLRYCSSTSCYAGARDYYYYYMDVW at 379, score = 9 + 7
umis assigned: [79, 88, 93, 104, 128, 144, 301, 384]
of which 8 are surviving nonsolos
reads assigned: 1837
start codons at 16, 37, 81, 425, 457
confident = true

TIG 2[bases=568]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
394-432 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 8 umis using 387 reads
cdr3 = CQQRSNWPSFTF at 368, score = 9 + 8
umis assigned: [23, 32, 48, 80, 111, 203, 249, 253, 255, 289] and 1 others
of which 10 are surviving nonsolos
reads assigned: 2491
start codons at 47, 252, 255, 474
confident = true

REJECT CONTIGS

TIG 1[bases=485]
5-54 ==> 4434-4483 on segment before IGHV1OR16-1 exon 1 [len=6000] (mis=1)
5-54 ==> 4391-4440 on segment before IGHV1OR16-2 exon 1 [len=6000] (mis=1)
52-79 ==> 101-128 on |77|IGHV1-46|L-REGION+V-REGION| [len=351] (mis=2)
79-261 ==> 129-311 on |77|IGHV1-46|L-REGION+V-REGION| [len=351] (mis=25)
332-383 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=2)
383-485 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
cdr3 = CARHRRDYGDYAQLNWFDPW at 292, score = 6 + 7
umis assigned: [334, 337]
of which 2 are surviving nonsolos
reads assigned: 298
start codons at 7, 26, 50, 144, 148, 170, 185, 214, 247, 281, 401, 462
confident = false
VJ delta = 10
not full
not full
now this is a cell
paired!

CGATATTGTAGTAGTACCAGCTGCTATGCCGGGGCTAGGGACTACTACTACTACTACATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG <==> AGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTTCATTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.436 = AGGGATGTCAAGGCTT-1

using 223 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[223]
surviving nonsolo ucounts = 1[223]
ids = [0]

====================================================================================

UMI info for barcode AGGGATGTCAAGGCTT-1 contig 1 = ATCACATAAC...
umi ATCGGTCAGG = 216 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=525]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=5)
409-429 ==> 0-20 on |10|IGHD1-26|D-REGION| [len=20] (mis=2)
425-473 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
473-525 ==> 0-52 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARDKVGATTFDYW at 400, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 58, 209, 256, 355
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.443 = AGGGATGTCAGGCCCA-1

using 8251 reads

====================================================================================

graph has 3657 edges initially, 52 edges after simplification

total ucounts = 475
nonsolo ucounts = 195[2^76, 3^28, 4^15, 5^18, 6^12, 7^5, 8^3, 9^2, 10, 12^2, 13, 14, 15, 53, 63, 95, 99, 106, 113, 117, 132, 136, 165, 202, 206, 212, 236, 259, 270, 271, 278, 281, 289, 296, 309, 326, 333, 344, 368, 376, 377, 482, 566]
surviving nonsolo ucounts = 27[53, 95, 99, 106, 117, 132, 136, 165, 202, 212, 236, 259, 270, 271, 278, 281, 289, 296, 309, 326, 333, 344, 368, 376, 377, 482, 566]
ids = [30, 428, 145, 248, 262, 352, 247, 355, 329, 131, ...]

====================================================================================

UMI info for barcode AGGGATGTCAGGCCCA-1 contig 1 = GCAGCGCCTG...
umi AATAACTCTT = 53 reads: +337 -13 +71 -9 non-validated
umi ATGTGTCATA = 275 reads: +429 -1 non-validated
umi CACAGTCATT = 260 reads: +430 validated
umi CGTAAATTCG = 234 reads: +430 validated
umi CGTACTTTGT = 117 reads: +415 -15 non-validated
umi GCGCGGGCTA = 203 reads: +430 validated
umi GTCCACTTCA = 282 reads: +430 validated
umi TTACTCAGTG = 275 reads: +430 validated

UMI info for barcode AGGGATGTCAGGCCCA-1 contig 2 = AGAGCTCTGG...
umi ACATTGGACC = 310 reads: +385 validated
umi AGAACTGGGG = 365 reads: +385 validated
umi AGCCCGTCTC = 334 reads: +385 validated
umi AGGTCATCGT = 376 reads: +385 validated
umi ATCTCAGCAC = 7 reads: -320 +4 -13XX +1 -4XX +1 -1XX +1 -1XX +1 -2XX +1 -1XX +1 -1XX +32 invalidated
umi ATTCCCCGGA = 100 reads: +385 validated
umi ATTTTAATTC = 279 reads: +385 validated
umi CAGATACCGG = 385 reads: +385 validated
umi CCCCCCACGA = 302 reads: -318X +6 -13XX +1 -4XX +1 -1XX +1 -1XX +1 -2XX +1 -1XX +1 -1XX +32 invalidated
umi CGCCGGTTGG = 299 reads: +385 validated
umi CGCTACGCGA = 107 reads: +338 -20 +2 -1 +24 non-validated
umi GATCCAGCGT = 286 reads: +385 validated
umi GCCCTAAGGT = 332 reads: +385 validated
umi GTAACAGCAT = 4 reads: -322 +2 -13X +1 -4X +1 -1XX +1 -1XX +1 -2XX +1 -1XX +1 -1XX +32 invalidated
umi TCGATCGGGT = 349 reads: +385 validated
umi TCTTTGCATT = 99 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=553]
0-52 ==> 0-52 on |206|IGHV6-1|5'UTR| [len=52] (mis=1)
52-417 ==> 0-365 on |207|IGHV6-1|L-REGION+V-REGION| [len=365] (mis=19)
434-482 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
482-553 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 6 umis using 119 reads
cdr3 = CARGGVAGTRNFDYW at 406, score = 8 + 7
umis assigned: [30, 141, 167, 260, 262, 329, 362, 457]
of which 8 are surviving nonsolos
reads assigned: 1662
start codons at 52, 96, 255, 293, 299
confident = true

TIG 2[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-374 ==> 0-330 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=13)
390-429 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 12 umis using 508 reads
cdr3 = CQDYGYSSCNF at 368, score = 9 + 7
umis assigned: [53, 85, 100, 108, 131, 145, 150, 182, 209, 245] and 6 others
of which 16 are surviving nonsolos
reads assigned: 3859
start codons at 44, 252, 378, 391, 471
confident = true
now this is a cell
paired!

CCCGACGACACGGCTGCGTATTTTTGTGCAAGGGGAGGAGTGGCTGGTACCCGAAATTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCGG <==> ATCAGTAAACTGGAGCCTGAAGATATTGCAGTGTATTACTGTCAGGACTATGGTTACTCCTCATGCAATTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.446 = AGGGATGTCATCGATG-1

using 86 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[5, 79]
surviving nonsolo ucounts = 1[79]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=406]
0-345 ==> 3-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=9)
346-385 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
385-406 ==> 0-21 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYGSSQPYTF at 321, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 73
start codons at 201, 205, 331
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.451 = AGGGATGTCATTGCGA-1

using 177 reads

====================================================================================

graph has 72 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 172]
surviving nonsolo ucounts = 1[172]
ids = [2]

====================================================================================

UMI info for barcode AGGGATGTCATTGCGA-1 contig 1 = CAGAGAGGAG...
umi CTAAATGGCT = 152 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=471]
0-73 ==> 6-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=5)
436-470 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CASRRGSW at 415, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 73, 229, 290, 308, 376
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.453 = AGGGATGTCCCTAATT-1

using 1159 reads

====================================================================================

graph has 1214 edges initially, 24 edges after simplification

total ucounts = 400
nonsolo ucounts = 166[2^63, 3^42, 4^25, 5^11, 6^6, 7^7, 8^3, 9^2, 11, 12^3, 15, 16, 313]
surviving nonsolo ucounts = 1[313]
ids = [383]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.454 = AGGGATGTCCGCAGTG-1

using 239 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[233]
surviving nonsolo ucounts = 1[233]
ids = [3]

====================================================================================

UMI info for barcode AGGGATGTCCGCAGTG-1 contig 1 = GGGGAGGAAT...
umi GCGCTTGTAG = 212 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=505]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-505 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.462 = AGGGATGTCCTTTACA-1

using 439 reads

====================================================================================

graph has 122 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 139, 295]
surviving nonsolo ucounts = 2[139, 295]
ids = [0, 2]

====================================================================================

UMI info for barcode AGGGATGTCCTTTACA-1 contig 1 = GACTGATCAG...
umi TATGCATCTC = 300 reads: +397 validated

UMI info for barcode AGGGATGTCCTTTACA-1 contig 2 = GGAGGAACTG...
umi ACTTGACACA = 139 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=567]
0-34 ==> 2-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=0)
34-394 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=1)
393-431 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
431-567 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CMQALQTPFTF at 370, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 294
start codons at 34, 67, 103, 191, 353, 373, 473
confident = false

TIG 2[bases=552]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=2)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 25 reads
cdr3 = CQQYNNWSWTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 138
start codons at 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.463 = AGGGATGTCGAACGGA-1

using 38 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2^2, 32]
surviving nonsolo ucounts = 1[32]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.468 = AGGGATGTCGTACCGG-1

using 48 reads

====================================================================================

graph has 28 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[7, 38]
surviving nonsolo ucounts = 1[38]
ids = [2]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.469 = AGGGATGTCGTCGTTC-1

using 191 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[6, 183]
surviving nonsolo ucounts = 1[183]
ids = [3]

====================================================================================

UMI info for barcode AGGGATGTCGTCGTTC-1 contig 1 = GGTAGCTCAG...
umi TATTTGCCTC = 178 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=525]
0-36 ==> 50-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
36-374 ==> 0-338 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=16)
389-427 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
427-525 ==> 0-98 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 33 reads
cdr3 = CHSYNNNNQGVF at 363, score = 6 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 174
start codons at 36, 99, 190, 241
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.471 = AGGGATGTCTAACGGT-1

using 647 reads

====================================================================================

graph has 166 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[6, 73, 251, 313]
surviving nonsolo ucounts = 3[73, 251, 313]
ids = [7, 1, 6]

====================================================================================

UMI info for barcode AGGGATGTCTAACGGT-1 contig 1 = AGGCTGATCA...
umi TTCATTTATT = 314 reads: +388 validated
umi TTCCCTCTCA = 69 reads: -345 +43 non-validated

UMI info for barcode AGGGATGTCTAACGGT-1 contig 2 = GCTCTGCTTC...
umi GGCCCTCTTA = 247 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=571]
0-47 ==> 0-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
47-398 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=12)
398-435 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
435-571 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQANSFPLTF at 374, score = 9 + 9
umis assigned: [6, 7]
of which 2 are surviving nonsolos
reads assigned: 379
start codons at 47, 53, 109, 122, 261, 279, 477
confident = false

TIG 2[bases=596]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-596 ==> 0-151 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 246
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.472 = AGGGATGTCTAACTTC-1

using 391 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[388]
surviving nonsolo ucounts = 1[388]
ids = [0]

====================================================================================

UMI info for barcode AGGGATGTCTAACTTC-1 contig 1 = GGAATCAGTC...
umi ACAGTACCGT = 388 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=1)
junction support: 1 umis using 58 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 382
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.476 = AGGGATGTCTCAAGTG-1

using 228 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 220]
surviving nonsolo ucounts = 1[220]
ids = [7]

====================================================================================

UMI info for barcode AGGGATGTCTCAAGTG-1 contig 1 = AGCTCTGAGA...
umi TTTTTACGGT = 220 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=579]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-579 ==> 0-76 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.486 = AGGGATGTCTTACCGC-1

using 194 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^3, 5, 178]
surviving nonsolo ucounts = 1[178]
ids = [7]

====================================================================================

UMI info for barcode AGGGATGTCTTACCGC-1 contig 1 = GGTGGTAGCT...
umi TCATTATGCA = 172 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=516]
0-39 ==> 47-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
39-392 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=13)
394-430 ==> 10-46 on |317|IGLJ7|J-REGION| [len=46] (mis=1)
430-516 ==> 0-86 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQSYDGNNQPVF at 366, score = 6 + 6
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 170
start codons at 39, 102, 376, 379
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.488 = AGGGATGTCTTGAGAC-1

using 5550 reads

====================================================================================

graph has 5130 edges initially, 62 edges after simplification

total ucounts = 1359
nonsolo ucounts = 941[2^205, 3^148, 4^138, 5^100, 6^69, 7^55, 8^52, 9^45, 10^30, 11^28, 12^16, 13^25, 14^10, 15^6, 16^5, 17^2, 18^3, 19, 20, 21, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.490 = AGGGTGAAGAAGGTGA-1

using 309 reads

====================================================================================

graph has 182 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3^2, 6, 293]
surviving nonsolo ucounts = 1[293]
ids = [4]

====================================================================================

UMI info for barcode AGGGTGAAGAAGGTGA-1 contig 1 = GGCTGGGGTC...
umi CCTCTCTTCT = 259 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=527]
42-394 ==> 0-352 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=22)
392-430 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
430-527 ==> 0-97 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CTSYTSSGTRVF at 366, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 42, 178, 199, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.491 = AGGGTGAAGACAAGCC-1

using 331 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[2, 322]
surviving nonsolo ucounts = 1[322]
ids = [5]

====================================================================================

UMI info for barcode AGGGTGAAGACAAGCC-1 contig 1 = GGCTGGGGTC...
umi TGTCGTTTCG = 311 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=572]
42-403 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
392-430 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
430-572 ==> 0-142 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CSSYTSSSTLVF at 366, score = 8 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 42, 199, 243, 250, 253, 562
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.493 = AGGGTGAAGAGTGAGA-1

using 119 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 4, 108]
surviving nonsolo ucounts = 1[108]
ids = [5]

====================================================================================

UMI info for barcode AGGGTGAAGAGTGAGA-1 contig 1 = GGGGTCACAA...
umi TCATAGAGGG = 106 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=532]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=5)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
429-532 ==> 0-103 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CCSYAGSSTYVLF at 362, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 103
start codons at 38, 177, 239, 246, 372, 390
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.496 = AGGGTGAAGATGTAAC-1

using 304 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 292]
surviving nonsolo ucounts = 1[292]
ids = [1]

====================================================================================

UMI info for barcode AGGGTGAAGATGTAAC-1 contig 1 = CCACATCCCT...
umi ACATAATCGG = 295 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=552]
0-42 ==> 0-42 on |72|IGHV1-3|5'UTR| [len=42] (mis=1)
42-395 ==> 0-353 on |73|IGHV1-3|L-REGION+V-REGION| [len=353] (mis=16)
397-425 ==> 0-28 on |15|IGHD2-21|D-REGION| [len=28] (mis=4)
428-481 ==> 10-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
481-552 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CARERHIVVVTTVPLYYAMDVW at 384, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 288
start codons at 42, 193, 198, 240, 245, 262, 339, 438
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.504 = AGGGTGAAGGACTGGT-1

using 253 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[253]
surviving nonsolo ucounts = 1[253]
ids = [0]

====================================================================================

UMI info for barcode AGGGTGAAGGACTGGT-1 contig 1 = GGCTTTCTGA...
umi TACCACCACT = 246 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=603]
36-173 ==> 0-137 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=25)
173-299 ==> 140-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=30)
420-466 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=12)
466-603 ==> 0-137 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
junction support: 1 umis using 32 reads
cdr3 = CARGPLVRGILGKGYYLDLW at 375, score = 8 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 244
start codons at 36, 80, 345
confident = false
see deletion of 3 bases at pos 137 on |197|IGHV4/OR15-8|L-REGION+V-REGION|
>vscore_12.504_77.1%
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTCCTGTCGCAGGGACAGCTGCGAGAGTGGGGCGGAGGCCTGTTGGAGACCTCGCAGACGCTGTCACTCACCTGTTCTGTTTACGGTCGAGTCTTCGCTGGCTCCTTCTGGACTTGGATCCGCCAATCCCCAGGCAAGGGGCCGGAGTGGATTGGAGAAATTGCTCAGGGTGGAAGACCCAACTACAACCCGGCCCTCCAGAGTCGCGTCACCATTTCAACTGACACGTCGCAGATCCAGTTCTCCCTGACTGTGAGGTCTATGACCGCCGCGGACTCGGCTACATATTACTGTGCCAGGGG
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.509 = AGGGTGAAGGGATCTG-1

using 240 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[239]
surviving nonsolo ucounts = 1[239]
ids = [1]

====================================================================================

UMI info for barcode AGGGTGAAGGGATCTG-1 contig 1 = GCTCTGCTTC...
umi CTAACAGGAA = 227 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=595]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-595 ==> 0-150 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 51, 205, 208, 259, 358, 385, 409, 577
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.533 = AGGGTGACACCATCCT-1

using 5146 reads

====================================================================================

graph has 3796 edges initially, 52 edges after simplification

total ucounts = 1487
nonsolo ucounts = 515[2^247, 3^107, 4^59, 5^28, 6^18, 7^18, 8^6, 9^7, 10^4, 11^5, 12, 13, 14^2, 16, 18, 54, 79, 127, 163, 186, 228, 242, 244, 424, 709]
surviving nonsolo ucounts = 9[18, 54, 79, 127, 163, 186, 242, 244, 709]
ids = [1418, 278, 829, 557, 595, 488, 970, 820, 744]

====================================================================================

UMI info for barcode AGGGTGACACCATCCT-1 contig 1 = GAAACAGAGC...
umi AGTTGTCACG = 54 reads: +388 validated
umi CCTCTGCACC = 131 reads: +388 validated
umi CGCTATTCAC = 113 reads: +34 -1 +2 -1X +4 -1XX +345 invalidated
umi CTTCGGCGAA = 719 reads: -191X +197 invalidated
umi GGTCCTTCAC = 245 reads: +388 validated
umi TCCGTTCTAA = 404 reads: -388 non-validated

UMI info for barcode AGGGTGACACCATCCT-1 contig 2 = GTCGGCGGTG...
umi CCAGCCAGTA = 187 reads: +406 validated
umi GATCCGCTGC = 239 reads: -308 +98 non-validated
umi GATTCGTGGG = 70 reads: -330X +76 invalidated
umi TTCTGTCTGT = 15 reads: -406 non-validated

GOOD CONTIGS

TIG 1[bases=637]
38-390 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=22)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
426-637 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 5 umis using 237 reads
cdr3 = CSAWDSSLNVWVF at 359, score = 7 + 8
umis assigned: [278, 557, 595, 744, 970, 1213]
of which 5 are surviving nonsolos
reads assigned: 1628
start codons at 38, 177, 367, 384
confident = true

TIG 2[bases=527]
0-50 ==> 30-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=0)
50-401 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=22)
406-456 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
456-527 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CVKEEVAFDIW at 392, score = 9 + 8
umis assigned: [488, 820, 829, 1418]
of which 4 are surviving nonsolos
reads assigned: 504
start codons at 50, 201, 206, 233, 267, 353, 437
confident = true
now this is a cell
paired!

AGCAGCCTGAGAGCTGAGGACACGGCTCTTTATTACTGTGTTAAAGAGGAGGTTGCTTTTGATATCTGGGGCCAAGGGACAATGGTCACCGTCTCTTCAG <==> GGACTCCAGCCTGACGACGAGGGTGACTATTACTGCTCAGCATGGGACAGCAGCCTCAATGTTTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.537 = AGGGTGACACTGAAGG-1

using 291 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 287]
surviving nonsolo ucounts = 1[287]
ids = [2]

====================================================================================

UMI info for barcode AGGGTGACACTGAAGG-1 contig 1 = AGGAGTCAGA...
umi GGACTTTACA = 263 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=512]
0-27 ==> 2-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=0)
27-378 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=28)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-512 ==> 0-97 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CLQDYTYPFSF at 354, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 261
start codons at 27, 33, 89, 102, 184, 187, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.539 = AGGGTGACACTTACGA-1

using 266 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[6, 10, 17, 228]
surviving nonsolo ucounts = 1[228]
ids = [2]

====================================================================================

UMI info for barcode AGGGTGACACTTACGA-1 contig 1 = GAAAGCATCA...
umi CCACACGACT = 210 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=541]
0-64 ==> 240-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=1)
64-417 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=20)
446-494 ==> 13-61 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
494-541 ==> 0-47 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CARGDRRAAAFYYHYMDVW at 406, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 64, 220, 262, 328, 361, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.547 = AGGGTGACAGCTCGCA-1

using 17797 reads

====================================================================================

graph has 6017 edges initially, 76 edges after simplification

total ucounts = 773
nonsolo ucounts = 343[2^122, 3^54, 4^35, 5^21, 6^20, 7^2, 8^9, 9^6, 10^3, 11^3, 12^2, 13^2, 15^2, 19, 20, 21, 23, 30, 37, 44, 60, 64, 65, 68, 86, 122, 157, 159, 169, 177, 189, 223, 232, 234, 238, 242^2, 247, 256, 259, 263, 272, 273, 278, 283, 285, 287, 289, 296, 298^2, 301, 307, 323^2, 326, 327^2, 330, 331, 342, 348, 349, 350, 356, 363, 364, 365, 378, 412, 413, 590, 619, 643, 721]
surviving nonsolo ucounts = 54[64, 65, 68, 86, 122, 157, 159, 169, 177, 189, 223, 232, 234, 238, 242^2, 247, 256, 259, 263, 272, 273, 278, 283, 285, 287, 289, 296, 298^2, 301, 307, 323^2, 326, 327^2, 330, 331, 342, 348, 349, 350, 356, 363, 364, 365, 378, 412, 413, 590, 619, 643, 721]
ids = [582, 18, 249, 251, 677, 532, 571, 254, 149, 489, ...]

====================================================================================

UMI info for barcode AGGGTGACAGCTCGCA-1 contig 1 = GGAGTCAGTC...
umi AACCAGCCAT = 359 reads: +394 validated
umi AATAGGCGCC = 247 reads: +394 validated
umi ACCGAGAGTC = 329 reads: +394 validated
umi ACCGGTCTAG = 284 reads: +394 validated
umi AGACATATGG = 329 reads: +394 validated
umi AGTATAGGTA = 233 reads: +394 validated
umi CATTAACTTG = 274 reads: +394 validated
umi CCCCTGCTCT = 299 reads: +394 validated
umi CCTAATTCAG = 335 reads: +394 validated
umi CGTTCTTCGT = 255 reads: +394 validated
umi GAAATCCGGT = 262 reads: +394 validated
umi GCGACTGTTT = 345 reads: +394 validated
umi GTAATACTCC = 190 reads: +394 validated
umi TAGTTCCCGG = 161 reads: +394 validated
umi TCCGGTCGTG = 586 reads: -188 +206 non-validated
umi TGGCCACGGT = 123 reads: +394 validated
umi TGTAGGACTA = 351 reads: +394 validated
umi TTGTCACAGG = 252 reads: +394 validated

UMI info for barcode AGGGTGACAGCTCGCA-1 contig 2 = AACAACCACA...
umi TCCTTTGGGT = 298 reads: +451 validated

GOOD CONTIGS

TIG 1[bases=556]
0-26 ==> 32-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
26-377 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=0)
383-420 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 18 umis using 825 reads
cdr3 = CQQLNSYPPSLTF at 353, score = 9 + 9
umis assigned: [13, 27, 53, 56, 85, 104, 218, 247, 271, 323] and 8 others
of which 18 are surviving nonsolos
reads assigned: 5127
start codons at 26, 32, 88, 237, 462
confident = true

TIG 2[bases=530]
0-51 ==> 10-61 on |84|IGHV1-69|5'UTR| [len=61] (mis=0)
51-404 ==> 0-353 on |85|IGHV1-69|L-REGION+V-REGION| [len=353] (mis=0)
439-502 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
502-530 ==> 0-28 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CARDLVLYSTPAGSRSYYYYYGMDVW at 393, score = 9 + 7
umis assigned: [627]
of which 1 are surviving nonsolos
reads assigned: 291
start codons at 51, 202, 249, 348, 459, 520
confident = true

REJECT CONTIGS

TIG 1[bases=549]
3-78 ==> 5665-5740 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=4)
375-413 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [12, 15, 18, 19, 24, 58, 107, 149, 165, 249] and 25 others
of which 35 are surviving nonsolos
reads assigned: 10293
start codons at 27, 33, 89, 102, 334, 455
confident = false
did not find CDR3
now this is a cell
paired!

GATCTGGTCCTCTATAGCACCCCGGCCGGCTCTCGATCTTACTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> AGCCTGCAGCCTGAAGATTTTGCAACTTATTACTGTCAACAGCTTAATAGTTACCCTCCGTCGCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.551 = AGGGTGACAGGTTTCA-1

using 273 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[271]
surviving nonsolo ucounts = 1[271]
ids = [2]

====================================================================================

UMI info for barcode AGGGTGACAGGTTTCA-1 contig 1 = CAGCTTCAGC...
umi TTGTATCTCA = 256 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=580]
0-48 ==> 66-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
48-401 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
398-436 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
436-580 ==> 0-144 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CASWDDSLRGRVF at 369, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 252
start codons at 48, 202, 352, 377, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.563 = AGGGTGACATTCTCAT-1

using 125 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 4[2^2, 5, 107]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.569 = AGGGTGAGTACCGCTG-1

using 14268 reads

====================================================================================

graph has 4307 edges initially, 48 edges after simplification

total ucounts = 350
nonsolo ucounts = 145[2^40, 3^21, 4^10, 5^14, 6, 7^4, 8^4, 9^2, 10, 20, 25, 35, 71, 106, 112, 120, 123, 182, 194, 206^2, 210, 218, 235, 242, 243, 247, 260, 261, 265, 269, 286, 288, 293, 296, 303, 308, 320, 327^2, 328, 329, 336, 344^2, 363, 369, 378, 386, 393, 404, 408, 422, 429, 431, 681, 773]
surviving nonsolo ucounts = 44[71, 112, 120, 123, 182, 194, 206^2, 210, 218, 235, 242, 243, 247, 260, 261, 265, 269, 286, 288, 293, 296, 303, 308, 320, 327^2, 328, 329, 336, 344^2, 363, 369, 378, 386, 393, 404, 408, 422, 429, 431, 681, 773]
ids = [256, 113, 301, 317, 187, 102, 190, 215, 24, 34, ...]

====================================================================================

UMI info for barcode AGGGTGAGTACCGCTG-1 contig 1 = TGGGGAGGAG...
umi AATGCTTCAG = 682 reads: +388 validated
umi ACTAGCCGTT = 330 reads: +388 validated
umi AGTGCTCTCC = 239 reads: +388 validated
umi ATGATTGCAT = 247 reads: +388 validated
umi CACTCACATA = 407 reads: +388 validated
umi CAGGATCGGC = 287 reads: +388 validated
umi CATATTGCAT = 292 reads: +388 validated
umi CATGTTGAAG = 113 reads: +379 -5 +4 non-validated
umi CCGTACTGTT = 250 reads: +388 validated
umi GCACATCTTC = 292 reads: +388 validated
umi GCACGGACGT = 199 reads: -348X +1 -1X +1 -3X +2 -1X +6 -2XX +15 -1XX +7 invalidated
umi GTGGATTCGA = 374 reads: +388 validated
umi TACCAAAGCC = 73 reads: +343 -1XX +37 -1 +1 -1 +4 invalidated
umi TAGATTTAGC = 332 reads: +388 validated
umi TAGTAGAGAT = 296 reads: +388 validated
umi TCATTTCTGA = 266 reads: +388 validated
umi TCCCCCCCTT = 331 reads: +388 validated
umi TCGGGTTTCA = 310 reads: +388 validated
umi TGTCATCCGC = 123 reads: -354X +2 -1X +6 -2X +15 -1XX +7 invalidated
umi TTATCACGCC = 247 reads: +388 validated
umi TTTCGTGAGT = 431 reads: +388 validated

UMI info for barcode AGGGTGAGTACCGCTG-1 contig 2 = TGGGGAGTGA...
umi AATTCCAGCT = 409 reads: +430 validated
umi ACACTTGGAG = 327 reads: +430 validated
umi ACATGGCCTT = 173 reads: +430 validated
umi ACCCGCCGTG = 263 reads: +428 -1X +1 invalidated
umi ACGTACCGGG = 221 reads: +421 -9 non-validated
umi ACGTGTTGGT = 322 reads: +430 validated
umi AGTGGCGCTA = 211 reads: +430 validated
umi ATAGCAGCTC = 345 reads: +430 validated
umi CAAGACTGGA = 306 reads: +430 validated
umi CAGTCTGCTA = 158 reads: +430 validated
umi CATGTTCCTG = 349 reads: +430 validated
umi CCACACTGTA = 774 reads: -183X +247 invalidated
umi CCCTATATGC = 377 reads: +430 validated
umi GATAGCACAT = 182 reads: +430 validated
umi GGATCGCGCT = 206 reads: +430 validated
umi TAATCAACCA = 341 reads: +430 validated
umi TATCCAGATA = 365 reads: +430 validated
umi TGAGCCCCAC = 389 reads: +430 validated
umi TGCAAGATGC = 119 reads: +390 -1 +3 -4 +9 -1 +8 -3 +5 -1 +3 -1 +1 non-validated
umi TGCTTATATC = 328 reads: +430 validated
umi TGTCTTCCTC = 264 reads: +430 validated
umi TTTAGGACCC = 334 reads: +430 validated
umi TTTGAGTTGG = 439 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=556]
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=4)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 17 umis using 865 reads
cdr3 = CQQSYSTSWTF at 359, score = 9 + 8
umis assigned: [15, 39, 61, 81, 96, 99, 106, 113, 131, 189] and 11 others
of which 21 are surviving nonsolos
reads assigned: 5995
start codons at 32, 38, 94, 107, 243, 462
confident = true

TIG 2[bases=526]
25-383 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=3)
392-419 ==> 0-27 on |18|IGHD3-10|D-REGION| [len=27] (mis=5)
409-455 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
455-526 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 21 umis using 653 reads
cdr3 = CAHTSFSRITMVRGLDYW at 370, score = 7 + 7
umis assigned: [16, 23, 24, 26, 34, 36, 62, 69, 85, 102] and 13 others
of which 23 are surviving nonsolos
reads assigned: 7078
start codons at 25, 69, 248, 251, 331, 340, 400
confident = true
now this is a cell
paired!

ACAGCCACATATTACTGTGCACACACTAGCTTTTCCCGTATTACTATGGTTCGGGGACTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCTCGTGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.574 = AGGGTGAGTAGATTAG-1

using 282 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[282]
surviving nonsolo ucounts = 1[282]
ids = [0]

====================================================================================

UMI info for barcode AGGGTGAGTAGATTAG-1 contig 1 = GGAGGAACTG...
umi CATTGGTTGG = 255 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=499]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=17)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
416-499 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CLQYSDWPRTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.578 = AGGGTGAGTATATGAG-1

using 152 reads

====================================================================================

graph has 148 edges initially, 4 edges after simplification

total ucounts = 38
nonsolo ucounts = 23[2^4, 3^5, 4^2, 5^2, 6, 7, 8^2, 9, 10^3, 11, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.591 = AGGGTGAGTGTGCGTC-1

using 16 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 8]
surviving nonsolo ucounts = 1[8]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.603 = AGGGTGAGTTGTACAC-1

using 150 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 6[2^2, 4, 7, 11, 114]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=443]
0-346 ==> 509-855 on rc of segment before IGHD3-22 exon 1 [len=1142] (mis=5)
374-422 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
422-443 ==> 0-21 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [3]
of which 0 are surviving nonsolos
reads assigned: 90
start codons at 302, 309
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.607 = AGGGTGATCACATACG-1

using 1893 reads

====================================================================================

graph has 2563 edges initially, 28 edges after simplification

total ucounts = 876
nonsolo ucounts = 394[2^156, 3^92, 4^62, 5^26, 6^28, 7^11, 8^5, 9^3, 10^3, 11^3, 12^2, 15^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.619 = AGGGTGATCCAGTATG-1

using 891 reads

====================================================================================

graph has 342 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[31, 324, 534]
surviving nonsolo ucounts = 2[324, 534]
ids = [4, 0]

====================================================================================

UMI info for barcode AGGGTGATCCAGTATG-1 contig 1 = GAGGAATCAG...
umi ACTTTAAGTA = 533 reads: +388 validated
umi GCATAGCCTT = 324 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 153 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [0, 4]
of which 2 are surviving nonsolos
reads assigned: 846
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.623 = AGGGTGATCGCATGGC-1

using 107 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2^4, 97]
surviving nonsolo ucounts = 1[97]
ids = [5]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.624 = AGGGTGATCGCCGTGA-1

using 183 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[180]
surviving nonsolo ucounts = 1[180]
ids = [1]

====================================================================================

UMI info for barcode AGGGTGATCGCCGTGA-1 contig 1 = ATCAGTCCCA...
umi CCCCGTTACA = 169 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=434]
0-23 ==> 4-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
411-434 ==> 0-23 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CLQYSSSPWTF at 350, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 167
start codons at 23, 29, 98, 234
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.626 = AGGGTGATCGGTCCGA-1

using 275 reads

====================================================================================

graph has 98 edges initially, 4 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 112, 156]
surviving nonsolo ucounts = 2[112, 156]
ids = [0, 6]

====================================================================================

UMI info for barcode AGGGTGATCGGTCCGA-1 contig 1 = GTGCTTTCTG...
umi AAGCAATTCG = 107 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=532]
16-387 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
404-452 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
452-532 ==> 0-80 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 16 reads
cdr3 = CARYFAFVNYYFDKW at 376, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 105
start codons at 16, 37, 81
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.635 = AGGGTGATCTGAGTGT-1

using 288 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 4^2, 271]
surviving nonsolo ucounts = 1[271]
ids = [2]

====================================================================================

UMI info for barcode AGGGTGATCTGAGTGT-1 contig 1 = GAGGAACTGC...
umi GCACTATTCT = 276 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQRSNWPLTF at 354, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 33, 238, 241, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.636 = AGGGTGATCTGGCGAC-1

using 1515 reads

====================================================================================

graph has 1066 edges initially, 26 edges after simplification

total ucounts = 274
nonsolo ucounts = 182[2^41, 3^36, 4^23, 5^17, 6^17, 7^14, 8^6, 9^5, 10^8, 11^2, 12^3, 13^2, 14, 15^2, 16, 19, 46, 167, 307]
surviving nonsolo ucounts = 1[307]
ids = [176]

====================================================================================

UMI info for barcode AGGGTGATCTGGCGAC-1 contig 1 = GGAGTCAGAC...
umi GCGCATCGGG = 279 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=495]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=0)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
420-495 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYNSYSPTWTF at 353, score = 8 + 8
umis assigned: [176]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 26, 32, 88, 101, 333, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.640 = AGGTCATAGAAACCAT-1

using 340 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[4, 6^2, 319]
surviving nonsolo ucounts = 1[319]
ids = [2]

====================================================================================

UMI info for barcode AGGTCATAGAAACCAT-1 contig 1 = GCTGGGGTCA...
umi ATGCTTCCAA = 297 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
0-41 ==> 121-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
41-381 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=20)
391-429 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
429-552 ==> 0-123 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CCAYAGTTTWVF at 365, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 41, 180, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.658 = AGGTCATAGGAGCGAG-1

using 256 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 249]
surviving nonsolo ucounts = 1[249]
ids = [6]

====================================================================================

UMI info for barcode AGGTCATAGGAGCGAG-1 contig 1 = GTAGCTCAGG...
umi TTTTCAACTC = 236 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=501]
0-35 ==> 51-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
35-373 ==> 0-338 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=16)
388-426 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
426-501 ==> 0-75 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CHSYNNNNQGVF at 362, score = 6 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 235
start codons at 35, 98, 189, 240
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.660 = AGGTCATAGGCTAGAC-1

using 316 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[314]
surviving nonsolo ucounts = 1[314]
ids = [1]

====================================================================================

REJECT CONTIGS

TIG 1[bases=553]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-31 ==> 5706-5737 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=1)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=4)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 314
start codons at 30, 238, 364, 459
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.665 = AGGTCATAGGGTATCG-1

using 158 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[157]
surviving nonsolo ucounts = 1[157]
ids = [0]

====================================================================================

UMI info for barcode AGGTCATAGGGTATCG-1 contig 1 = AGCTTCAGCT...
umi GCAACGTCGC = 148 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=471]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=16)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-471 ==> 0-36 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 146
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.669 = AGGTCATAGTATCGAA-1

using 298 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 292]
surviving nonsolo ucounts = 1[292]
ids = [4]

====================================================================================

UMI info for barcode AGGTCATAGTATCGAA-1 contig 1 = ACAAGAGGCA...
umi TCAGAATTAA = 282 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=555]
0-31 ==> 20-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
31-387 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
387-425 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
425-555 ==> 0-130 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQSYDSSLSGLYVF at 355, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 31, 185, 188, 239, 338, 365, 389
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.671 = AGGTCATAGTTCGCAT-1

using 23 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.675 = AGGTCATCAAGCGAGT-1

using 96 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 1[92]
ids = [1]

====================================================================================

UMI info for barcode AGGTCATCAAGCGAGT-1 contig 1 = GATCAGGACT...
umi CCGTAATCTG = 88 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=471]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
427-471 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CMQALQTPLTF at 366, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 86
start codons at 30, 63, 99, 187, 349, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.688 = AGGTCATCACGTAAGG-1

using 23 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.689 = AGGTCATCACTACAGT-1

using 624 reads

====================================================================================

graph has 929 edges initially, 6 edges after simplification

total ucounts = 305
nonsolo ucounts = 116[2^51, 3^26, 4^13, 5^8, 6^6, 7^2, 8^2, 10^3, 11^2, 12, 13, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.693 = AGGTCATCAGAAGCAC-1

using 402 reads

====================================================================================

graph has 235 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 10, 384]
surviving nonsolo ucounts = 1[384]
ids = [7]

====================================================================================

UMI info for barcode AGGTCATCAGAAGCAC-1 contig 1 = GAGTCAGACC...
umi TGATTGGCTT = 393 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=549]
0-31 ==> 22-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=0)
31-376 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=1)
381-413 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=0)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 76 reads
cdr3 = CQQYYSYPPHF at 352, score = 9 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 380
start codons at 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.696 = AGGTCATCAGAGTGTG-1

using 823 reads

====================================================================================

graph has 310 edges initially, 26 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2^4, 159, 241, 413]
surviving nonsolo ucounts = 3[159, 241, 413]
ids = [1, 7, 4]

====================================================================================

UMI info for barcode AGGTCATCAGAGTGTG-1 contig 1 = AGAAGAGCTG...
umi GCGGACCGCC = 417 reads: +388 validated

UMI info for barcode AGGTCATCAGAGTGTG-1 contig 2 = GGGGGTCTCA...
umi ACATTAGTTG = 31 reads: -12X +1 -2XX +2 -2XX +19 -2XX +1 -2XX +21 -2XX +11 -2XX +4 -1XX +1 -1XX +7 -1XX +1 -1X +2 -1X +1 -122X +14 -2XX +2 -1XX +1 -1XX +2 -1XX +22 -1XX +1 -1XX +1 -1XX +9 -1XX +5 -2XX +3 -35X +1 -5X +1 -1X +1 -5X +1 -3X +1 -3X +3 -1X +16 -1 +14 invalidated
umi TCATTGCTAC = 234 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=558]
0-34 ==> 18-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
34-382 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
383-422 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
422-558 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 75 reads
cdr3 = CQQYGSSPPYTF at 358, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 410
start codons at 34, 242, 368, 464
confident = false

TIG 2[bases=536]
39-387 ==> 0-348 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=6)
386-424 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
424-536 ==> 0-112 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 42 reads
cdr3 = CSSYTSSSVVF at 363, score = 8 + 8
umis assigned: [1, 7]
of which 2 are surviving nonsolos
reads assigned: 260
start codons at 39, 196, 240, 250
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.699 = AGGTCATCAGCGTAAG-1

using 305 reads

====================================================================================

graph has 140 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3, 4, 8, 288]
surviving nonsolo ucounts = 1[288]
ids = [2]

====================================================================================

UMI info for barcode AGGTCATCAGCGTAAG-1 contig 1 = TGGGGCTGGG...
umi GTATACACTT = 279 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
45-399 ==> 0-354 on |341|IGLV2-18|L-REGION+V-REGION| [len=354] (mis=6)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
433-499 ==> 0-66 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CSSYTTSTTWVF at 369, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 276
start codons at 45, 246, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.700 = AGGTCATCAGCTGGCT-1

using 436 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[434]
surviving nonsolo ucounts = 1[434]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=319]
0-70 ==> 286-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=4)
70-108 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
108-319 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYGSSLSGLYVF at 38, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 428
start codons at 21, 48, 72, 240
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.701 = AGGTCATCAGGCTGAA-1

using 542 reads

====================================================================================

graph has 210 edges initially, 12 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[2, 3, 22, 226, 287]
surviving nonsolo ucounts = 3[22, 226, 287]
ids = [0, 1, 5]

====================================================================================

UMI info for barcode AGGTCATCAGGCTGAA-1 contig 1 = GAGGAATCAG...
umi TGTATTACCT = 285 reads: +388 validated

UMI info for barcode AGGTCATCAGGCTGAA-1 contig 2 = GCTCTGCCTC...
umi AATGAAGTAT = 5 reads: -21 +3 -1X +1 -1X +6 -1XX +4 -3XX +23 -2XX +5 -1XX +5 -2XX +14 -1X +1 -299 invalidated
umi AGGTTACGTC = 212 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 280
start codons at 28, 34, 103, 239, 458
confident = false

TIG 2[bases=538]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=1)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=9)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
445-538 ==> 0-93 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQSYDTSLSGSNVF at 375, score = 8 + 8
umis assigned: [0, 1]
of which 2 are surviving nonsolos
reads assigned: 216
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.708 = AGGTCATCATGAGCGA-1

using 270 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 266]
surviving nonsolo ucounts = 1[266]
ids = [1]

====================================================================================

UMI info for barcode AGGTCATCATGAGCGA-1 contig 1 = GGGAGGAGTC...
umi AGTATTTCCA = 267 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=554]
30-383 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=15)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQSYTPLLTF at 357, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 263
start codons at 30, 36, 105, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.714 = AGGTCATCATTGTGCA-1

using 22 reads

====================================================================================

graph has 18 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.717 = AGGTCATGTAAGAGAG-1

using 380 reads

====================================================================================

graph has 106 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[124, 253]
surviving nonsolo ucounts = 2[124, 253]
ids = [3, 4]

====================================================================================

UMI info for barcode AGGTCATGTAAGAGAG-1 contig 1 = AGACCCTGTC...
umi TCTCCATAGT = 249 reads: +382 validated

UMI info for barcode AGGTCATGTAAGAGAG-1 contig 2 = GGGGAGGAAC...
umi GTGATTCGTA = 122 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=544]
0-26 ==> 27-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=0)
26-371 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=1)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYYSYPYTF at 347, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 247
start codons at 26, 82, 95, 231, 450
confident = false

TIG 2[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=1)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CQQRSNWPYTF at 357, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 121
start codons at 36, 241, 244, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.721 = AGGTCATGTCAAAGAT-1

using 340 reads

====================================================================================

graph has 186 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 1[322]
surviving nonsolo ucounts = 1[322]
ids = [9]

====================================================================================

UMI info for barcode AGGTCATGTCAAAGAT-1 contig 1 = GAGGAATCAG...
umi AATTCCCATT = 1 reads: -231 +5 -2 +11 -1 +1 -1 +9 -1 +6 -1 +5 -1 +12 -101 non-validated
umi ACGTTTGGTT = 1 reads: -217 +2 -1 +5 -1 +1 -2 +4 -1 +1 -1 +3 -1 +1 -1 +3 -1 +1 -1 +1 -2 +4 -2X +6 -1 +3 -2 +2 -117 invalidated
umi CCGAAAGCCT = 320 reads: +388 validated
umi TCTTCTTAGT = 1 reads: -251 +7 -1X +3 -1 +1 -1 +2 -2 +2 -2 +1 -1 +3 -1 +3 -1 +10 -1 +1 -1X +4 -1 +6 -81 invalidated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
378-416 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 62 reads
cdr3 = CLQHKSYPFTF at 355, score = 9 + 8
umis assigned: [3, 5, 9, 15]
of which 1 are surviving nonsolos
reads assigned: 317
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.724 = AGGTCATGTCATCGGC-1

using 237 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 233]
surviving nonsolo ucounts = 1[233]
ids = [2]

====================================================================================

UMI info for barcode AGGTCATGTCATCGGC-1 contig 1 = AGGAGTCAGA...
umi TTTTTGTTCC = 216 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
415-492 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CQQYNGQSRAF at 354, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 27, 33, 102, 238, 241, 334, 367, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.726 = AGGTCATGTCCGAGTC-1

using 448 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 6, 438]
surviving nonsolo ucounts = 1[438]
ids = [2]

====================================================================================

UMI info for barcode AGGTCATGTCCGAGTC-1 contig 1 = TGGGGGCTTT...
umi ACGGCAGCCC = 401 reads: +463 validated

GOOD CONTIGS

TIG 1[bases=501]
19-396 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=3)
419-482 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=3)
482-501 ==> 0-19 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CARGSSSSLVSWNYYYYGMDVW at 385, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 397
start codons at 19, 28, 40, 84, 439
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.727 = AGGTCATGTCCGCTGA-1

using 397 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[393]
surviving nonsolo ucounts = 1[393]
ids = [2]

====================================================================================

UMI info for barcode AGGTCATGTCCGCTGA-1 contig 1 = GGAGTCAGTC...
umi ATTCTGCGAT = 393 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 5-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
26-377 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
375-414 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 66 reads
cdr3 = CHQYESVPYTF at 353, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 389
start codons at 26, 32, 88, 101, 240, 336, 363, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.734 = AGGTCATGTGATGTGG-1

using 912 reads

====================================================================================

graph has 1323 edges initially, 16 edges after simplification

total ucounts = 366
nonsolo ucounts = 176[2^66, 3^51, 4^18, 5^16, 6^11, 7^6, 8, 9^2, 12, 13, 17, 18, 91]
surviving nonsolo ucounts = 1[91]
ids = [199]

====================================================================================

UMI info for barcode AGGTCATGTGATGTGG-1 contig 1 = GCTCTGCTTC...
umi GCATAGTCTC = 83 reads: +391 -1 +2 non-validated

GOOD CONTIGS

TIG 1[bases=460]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=8)
407-445 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
junction support: 1 umis using 12 reads
cdr3 = CQSYDRSLSGSLVF at 375, score = 8 + 9
umis assigned: [199]
of which 1 are surviving nonsolos
reads assigned: 81
start codons at 51, 205, 208, 259, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.740 = AGGTCATGTGTTGAGG-1

using 147 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 5[3, 5, 6, 24, 107]
surviving nonsolo ucounts = 2[24, 107]
ids = [5, 4]

====================================================================================

UMI info for barcode AGGTCATGTGTTGAGG-1 contig 1 = CTCAGTCAGG...
umi CGTTCATACG = 100 reads: +385 validated
umi TAAATTAGGT = 21 reads: -11 +256 -34 +84 non-validated

GOOD CONTIGS

TIG 1[bases=475]
17-370 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=5)
363-402 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
402-475 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CQQSYSTYTF at 344, score = 9 + 8
umis assigned: [4, 5]
of which 2 are surviving nonsolos
reads assigned: 120
start codons at 17, 23, 79, 92, 228, 444
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.742 = AGGTCATGTTATCCGA-1

using 1009 reads

====================================================================================

graph has 1494 edges initially, 22 edges after simplification

total ucounts = 470
nonsolo ucounts = 188[2^74, 3^42, 4^28, 5^16, 6^5, 7^6, 8^6, 9^4, 10^2, 11, 12, 13, 14, 35]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.746 = AGGTCATGTTGGTGGA-1

using 324 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3, 4, 7, 305]
surviving nonsolo ucounts = 2[4, 305]
ids = [7, 4]

====================================================================================

UMI info for barcode AGGTCATGTTGGTGGA-1 contig 1 = GAGGAATCAG...
umi GTTCCCGCTG = 291 reads: +388 validated
umi TACGAAATGA = 4 reads: -11 +57 -90 +15 -1 +27 -1X +12 -14 +5 -1 +2 -1 +21 -1 +5 -1 +19 -104 invalidated

GOOD CONTIGS

TIG 1[bases=495]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-495 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [4, 7]
of which 2 are surviving nonsolos
reads assigned: 292
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.761 = AGGTCATTCCAAATGC-1

using 656 reads

====================================================================================

graph has 320 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[252, 400]
surviving nonsolo ucounts = 2[252, 400]
ids = [0, 1]

====================================================================================

UMI info for barcode AGGTCATTCCAAATGC-1 contig 1 = AAAAACCACA...
umi AAAGTATATG = 251 reads: +436 validated
umi CCACTCCCAG = 408 reads: -36X +1 -6XX +2 -2XX +389 invalidated

GOOD CONTIGS

TIG 1[bases=646]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=23)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
486-646 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 2 umis using 94 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [0, 1]
of which 2 are surviving nonsolos
reads assigned: 642
start codons at 50, 248, 253, 270, 314, 347, 540
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.764 = AGGTCATTCCCTGACT-1

using 47 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[4, 5, 33]
surviving nonsolo ucounts = 1[33]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.765 = AGGTCATTCCGCATCT-1

using 281 reads

====================================================================================

graph has 99 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[279]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.784 = AGGTCATTCTTACCGC-1

using 399 reads

====================================================================================

graph has 190 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 394]
surviving nonsolo ucounts = 1[394]
ids = [3]

====================================================================================

UMI info for barcode AGGTCATTCTTACCGC-1 contig 1 = AGAGCTCTGG...
umi TTAAGGCCAG = 366 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=513]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
393-432 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
432-513 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQYGSSPPWSF at 368, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 357
start codons at 44, 252, 378, 474
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.790 = AGGTCCGAGAGCCCAA-1

using 128 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2^3, 3, 113]
surviving nonsolo ucounts = 1[113]
ids = [9]

====================================================================================

UMI info for barcode AGGTCCGAGAGCCCAA-1 contig 1 = GGAGCCTTAG...
umi TGTTTTACAA = 114 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=563]
0-67 ==> 12-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
67-420 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
444-491 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
491-563 ==> 0-72 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARDRAATARLGGMDVW at 409, score = 9 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 110
start codons at 67, 218, 223, 370, 448
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.796 = AGGTCCGAGCATGGCA-1

using 323 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 1[314]
surviving nonsolo ucounts = 1[314]
ids = [9]

====================================================================================

UMI info for barcode AGGTCCGAGCATGGCA-1 contig 1 = GGAGGAGTCA...
umi TTGTTACACG = 314 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=553]
0-29 ==> 0-29 on |234|IGKV1-6|5'UTR| [len=29] (mis=1)
29-380 ==> 0-351 on |235|IGKV1-6|L-REGION+V-REGION| [len=351] (mis=28)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CLQDYTYPFSF at 356, score = 9 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 309
start codons at 29, 35, 91, 104, 186, 189, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.800 = AGGTCCGAGCTCAACT-1

using 7502 reads

====================================================================================

graph has 3895 edges initially, 58 edges after simplification

total ucounts = 1127
nonsolo ucounts = 395[2^174, 3^83, 4^59, 5^22, 6^17, 7^7, 8^4, 9^3, 10^2, 11, 12, 13, 17, 21, 89, 103, 125, 144, 161, 168, 223, 231, 236, 261, 263, 280, 287, 328, 352, 371, 375, 718, 808]
surviving nonsolo ucounts = 17[89, 125, 144, 161, 168, 223, 231, 236, 261, 263, 280, 287, 328, 352, 371, 375, 808]
ids = [22, 281, 451, 860, 846, 697, 962, 852, 37, 1024, ...]

====================================================================================

UMI info for barcode AGGTCCGAGCTCAACT-1 contig 1 = AGTGACTCCT...
umi AACATACTAT = 263 reads: +439 validated
umi ATATCCTTCC = 286 reads: +439 validated
umi ATTTTTCCGC = 127 reads: +439 validated
umi CGCTCGTCAC = 146 reads: +439 validated
umi CTAGGCTACC = 281 reads: +439 validated
umi GCTACTTCCT = 873 reads: -406 +1 -5XX +1 -2XX +1 -1XX +1 -10XX +1 -2XX +1 -2XX +1 -3XX +1 invalidated
umi TAGTCACGCG = 171 reads: +439 validated
umi TATCACCATA = 162 reads: +439 validated

UMI info for barcode AGGTCCGAGCTCAACT-1 contig 2 = GAGCTACAAC...
umi GGCCACCCGT = 234 reads: +123 -1XX +276 invalidated
umi TAGTTGTCGG = 236 reads: +400 validated
umi TCATGTGCGC = 325 reads: +400 validated
umi TCTCGTGCGT = 232 reads: +400 validated
umi TGTAGCATGT = 261 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=641]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=14)
408-459 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
459-641 ==> 0-182 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
junction support: 7 umis using 190 reads
cdr3 = CAHRRACSGGRCFPHGGFDPW at 365, score = 7 + 7
umis assigned: [37, 216, 281, 451, 520, 672, 846, 860]
of which 7 are surviving nonsolos
reads assigned: 1983
start codons at 20, 243, 246, 323, 326, 335
confident = true

TIG 2[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=10)
392-430 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 179 reads
cdr3 = CQQYYSHPLTF at 369, score = 9 + 9
umis assigned: [697, 852, 903, 962, 1024]
of which 5 are surviving nonsolos
reads assigned: 1264
start codons at 30, 99, 352, 472
confident = true

REJECT CONTIGS

TIG 1[bases=528]
3-84 ==> 5665-5746 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=3)
27-284 ==> 0-257 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=26)
284-357 ==> 280-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=9) [SHIFT!]
354-392 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=2)
392-528 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [631, 932]
of which 2 are surviving nonsolos
reads assigned: 711
start codons at 27, 33, 89, 102, 193, 390, 434
confident = false
did not find CDR3

TIG 2[bases=308]
11-36 ==> 4932-4957 on rc of segment before IGLL1 exon 1 [len=6445] (mis=0)
59-97 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
95-143 ==> 6397-6445 on rc of segment before IGLL1 exon 1 [len=6445] (mis=4)
97-308 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
umis assigned: [601]
of which 1 are surviving nonsolos
reads assigned: 796
start codons at 61, 229
confident = false
did not find CDR3

TIG 3[bases=371]
1-32 ==> 317-348 on rc of segment before IGHJ5 exon 1 [len=348] (mis=0)
82-239 ==> 0-157 on rc of segment before IGHJ3P exon 1 [len=157] (mis=1)
237-300 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
300-371 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [22]
of which 1 are surviving nonsolos
reads assigned: 89
start codons at 89, 123, 162, 217, 257
confident = false
did not find CDR3
now this is a cell
paired!

TATTACTGTGCACACAGACGGGCCTGTAGTGGTGGTAGGTGTTTCCCTCACGGGGGCTTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAGTATTATAGTCATCCTCTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.801 = AGGTCCGAGCTCCTCT-1

using 86 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 5, 73]
surviving nonsolo ucounts = 1[73]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.803 = AGGTCCGAGGAACTGC-1

using 171 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 165]
surviving nonsolo ucounts = 1[165]
ids = [3]

====================================================================================

REJECT CONTIGS

TIG 1[bases=433]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
0-33 ==> 5967-6000 on rc of segment after IGKV3-11 exon 1 [len=6000] (mis=0)
6-58 ==> 5709-5761 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=3)
33-333 ==> 0-300 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=17)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
cdr3 = CQLRRNWPRGTF at 354, score = 6 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 140
start codons at 33, 238
confident = false
not full
full length stopped transcript of length 433
frameshifted full length stopped transcript of length 433
VJ delta = 13
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.805 = AGGTCCGAGGACTGGT-1

using 127 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[127]
surviving nonsolo ucounts = 1[127]
ids = [0]

====================================================================================

UMI info for barcode AGGTCCGAGGACTGGT-1 contig 1 = GCTGGGGTCA...
umi ACGCATTCAA = 123 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=559]
0-41 ==> 121-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
41-381 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
435-559 ==> 0-124 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 19 reads
cdr3 = CCSEAGGGVPGLLF at 365, score = 7 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 120
start codons at 41, 195
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.817 = AGGTCCGCAAGCCATT-1

using 628 reads

====================================================================================

graph has 268 edges initially, 36 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^3, 4, 285, 330]
surviving nonsolo ucounts = 2[285, 330]
ids = [7, 5]

====================================================================================

UMI info for barcode AGGTCCGCAAGCCATT-1 contig 1 = GGAGTCAGAC...
umi GGTACGTTGT = 331 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQQYNSYPLTF at 353, score = 8 + 9
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 323
start codons at 26, 32, 88, 101, 237, 240, 333, 456
confident = false

REJECT CONTIGS

TIG 1[bases=531]
0-75 ==> 5665-5740 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
24-377 ==> 0-353 on |261|IGKV1D-8|L-REGION+V-REGION| [len=353] (mis=2)
356-395 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=17)
395-531 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 282
start codons at 24, 30, 86, 99, 162, 235, 437
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.821 = AGGTCCGCACACCGCA-1

using 17 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.834 = AGGTCCGCACGTGAGA-1

using 111 reads

====================================================================================

graph has 120 edges initially, 4 edges after simplification

total ucounts = 23
nonsolo ucounts = 14[2^2, 3, 4, 5^2, 6, 8^2, 10^3, 12, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.842 = AGGTCCGCATAGACTC-1

using 7172 reads

====================================================================================

graph has 4298 edges initially, 48 edges after simplification

total ucounts = 878
nonsolo ucounts = 377[2^155, 3^99, 4^38, 5^20, 6^15, 7^7, 8^4, 9^4, 10^5, 11^3, 12, 13^3, 15, 16, 18, 20, 32, 116, 129, 168, 184, 193, 194, 196, 245, 246, 258, 267, 283^2, 287, 372, 417, 736, 796]
surviving nonsolo ucounts = 19[32, 116, 129, 168, 184, 193, 194, 196, 245, 246, 258, 267, 283^2, 287, 372, 417, 736, 796]
ids = [626, 732, 173, 37, 254, 0, 292, 741, 241, 604, ...]

====================================================================================

UMI info for barcode AGGTCCGCATAGACTC-1 contig 1 = GAGAGAGGAG...
umi ATTCATGCCA = 347 reads: +433 validated
umi ATTTATACTT = 287 reads: +433 validated
umi CTAGTGAGCT = 250 reads: +433 validated
umi GCCTCTGCCT = 290 reads: +433 validated
umi GGCTGTGTGG = 343 reads: +433 validated
umi GTTTTCCGTC = 26 reads: -432X +1 invalidated

UMI info for barcode AGGTCCGCATAGACTC-1 contig 2 = TCTGAGGATA...
umi AACTTTGGCT = 166 reads: +383 -6 +2 non-validated
umi ATACCCACTC = 130 reads: +391 validated
umi CACCGGACTT = 178 reads: +391 validated
umi CCATGCCCAG = 192 reads: +391 validated
umi CTACGCTCGG = 257 reads: +391 validated
umi GCAGATACAC = 800 reads: -343 +1 -2X +1 -2XX +1 -3XX +1 -1XX +36 invalidated
umi GGCCCCTCAC = 740 reads: -349X +1 -3XX +1 -1XX +36 invalidated
umi GGGCGTCACT = 279 reads: +391 validated
umi GTCTCAACTA = 245 reads: +391 validated
umi TCTATTCGGG = 143 reads: +33 -1 +3 -6XX +348 invalidated

GOOD CONTIGS

TIG 1[bases=608]
0-73 ==> 6-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=0)
443-506 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=4)
506-608 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 5 umis using 121 reads
cdr3 = CAKERVAAAGSYYYYGMDVW at 415, score = 9 + 7
umis assigned: [213, 221, 398, 520, 555, 626]
of which 6 are surviving nonsolos
reads assigned: 1507
start codons at 73, 224, 229, 376, 463, 524, 585
confident = true

TIG 2[bases=688]
0-86 ==> 0-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
86-439 ==> 0-353 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=2)
439-477 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
477-688 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 7 umis using 181 reads
cdr3 = CQSYDSSNHRVF at 413, score = 6 + 8
umis assigned: [37, 173, 254, 292, 393, 507, 549, 559, 604, 741]
of which 10 are surviving nonsolos
reads assigned: 3082
start codons at 86, 149, 240, 291, 423
confident = true
now this is a cell
paired!

GTATATTACTGTGCGAAAGAAAGGGTAGCAGCAGCTGGGAGCTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> TCTGGACTGAAGACTGAGGACGAGGCTGACTACTACTGTCAGTCTTATGATAGCAGCAATCATCGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.846 = AGGTCCGCATCACGAT-1

using 303 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[302]
surviving nonsolo ucounts = 1[302]
ids = [1]

====================================================================================

UMI info for barcode AGGTCCGCATCACGAT-1 contig 1 = GACTGATCAG...
umi TCCGGGCCCT = 276 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=506]
0-34 ==> 2-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=0)
34-394 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=1)
392-431 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
431-506 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CMQALQTPCSF at 370, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 274
start codons at 34, 67, 103, 191, 353, 373, 473
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.863 = AGGTCCGGTAGAAAGG-1

using 332 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 5, 319]
surviving nonsolo ucounts = 1[319]
ids = [1]

====================================================================================

UMI info for barcode AGGTCCGGTAGAAAGG-1 contig 1 = GTCAGTCTCA...
umi AAACGTGCTC = 320 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=4)
373-411 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQSYSTRFTF at 350, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 316
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.864 = AGGTCCGGTAGAAGGA-1

using 18 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[18]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.867 = AGGTCCGGTAGGCATG-1

using 499 reads

====================================================================================

graph has 246 edges initially, 6 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 231, 265]
surviving nonsolo ucounts = 2[231, 265]
ids = [3, 0]

====================================================================================

UMI info for barcode AGGTCCGGTAGGCATG-1 contig 1 = GGGCCTCAGG...
umi ACGGGCCCAA = 263 reads: +391 validated
umi TTCCGAGTGA = 234 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=584]
37-390 ==> 0-353 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
390-428 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
428-584 ==> 0-156 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 61 reads
cdr3 = CSSYTSSSTLRVF at 361, score = 8 + 8
umis assigned: [0, 3]
of which 2 are surviving nonsolos
reads assigned: 483
start codons at 37, 194, 238, 245, 248
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.870 = AGGTCCGGTCAACTGT-1

using 273 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 4, 263]
surviving nonsolo ucounts = 1[263]
ids = [3]

====================================================================================

UMI info for barcode AGGTCCGGTCAACTGT-1 contig 1 = GAATCAGTCC...
umi CTGTACCGAG = 247 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=481]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-481 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.873 = AGGTCCGGTCTAGCGC-1

using 258 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 2[3, 246]
surviving nonsolo ucounts = 1[246]
ids = [7]

====================================================================================

UMI info for barcode AGGTCCGGTCTAGCGC-1 contig 1 = GCTCTGCTTC...
umi GGTCTTGCAA = 240 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=516]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-516 ==> 0-71 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 232
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.877 = AGGTCCGGTCTTGTCC-1

using 213 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 5[2^3, 3, 186]
surviving nonsolo ucounts = 1[186]
ids = [17]

====================================================================================

UMI info for barcode AGGTCCGGTCTTGTCC-1 contig 1 = GAGGAGTCAG...
umi GTCAACCAGC = 166 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=479]
28-381 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=0)
381-419 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
419-479 ==> 0-60 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQSYSTPPITF at 355, score = 9 + 8
umis assigned: [17]
of which 1 are surviving nonsolos
reads assigned: 162
start codons at 28, 34, 90, 103, 239, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.881 = AGGTCCGGTGATAAGT-1

using 196 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[2, 190]
surviving nonsolo ucounts = 1[190]
ids = [3]

====================================================================================

UMI info for barcode AGGTCCGGTGATAAGT-1 contig 1 = GCTGGGGTCT...
umi GCCGTTCTAC = 187 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-395 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=3)
391-429 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
429-551 ==> 0-122 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CSSYAGSNNLVF at 365, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 183
start codons at 41, 198, 242, 249, 348, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.892 = AGGTCCGTCAAACGGG-1

using 123 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[3, 114]
surviving nonsolo ucounts = 1[114]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.907 = AGGTCCGTCCTAGGGC-1

using 60 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 43
nonsolo ucounts = 10[2^8, 5, 6]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.916 = AGGTCCGTCTCGGACG-1

using 386 reads

====================================================================================

graph has 184 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2^4, 163, 209]
surviving nonsolo ucounts = 2[163, 209]
ids = [0, 6]

====================================================================================

UMI info for barcode AGGTCCGTCTCGGACG-1 contig 1 = GACTCCTGTG...
umi CTATTAAAAG = 160 reads: +418 validated

UMI info for barcode AGGTCCGTCTCGGACG-1 contig 2 = GTCAGACTCA...
umi AAACCCTAGC = 162 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
17-367 ==> 0-350 on |94|IGHV2-26|L-REGION+V-REGION| [len=357] (mis=24)
384-435 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=5)
435-492 ==> 0-57 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CASAAAPGDWFDPW at 362, score = 7 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 157
start codons at 17, 173, 240, 332
confident = false

TIG 2[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQQYNSYPLTF at 350, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 161
start codons at 23, 29, 85, 98, 234, 237, 330, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.919 = AGGTCCGTCTGGCGTG-1

using 261 reads

====================================================================================

graph has 60 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[260]
surviving nonsolo ucounts = 1[260]
ids = [1]

====================================================================================

UMI info for barcode AGGTCCGTCTGGCGTG-1 contig 1 = GGGGAGGAAC...
umi GTGAGCTTCA = 258 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=554]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=2)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQRSNWLFTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 257
start codons at 36, 241, 244, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.922 = AGGTCCGTCTTATCTG-1

using 1620 reads

====================================================================================

graph has 1982 edges initially, 24 edges after simplification

total ucounts = 873
nonsolo ucounts = 320[2^154, 3^68, 4^40, 5^23, 6^13, 7^8, 8^6, 9^3, 10, 11, 14, 18^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.937 = AGTAGTCAGATCACGG-1

using 696 reads

====================================================================================

graph has 1111 edges initially, 8 edges after simplification

total ucounts = 359
nonsolo ucounts = 122[2^51, 3^27, 4^16, 5^10, 6^6, 7^6, 10, 12^2, 13, 14, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.940 = AGTAGTCAGCAATATG-1

using 291 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[288]
surviving nonsolo ucounts = 1[288]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCAGCAATATG-1 contig 1 = GAGAAGAGCT...
umi CAAGGTTCTT = 262 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=516]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
420-516 ==> 0-96 on |212|IGKC|C-REGION| [len=320] (mis=2)
junction support: 1 umis using 45 reads
cdr3 = CQQYGSSPVTF at 359, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 257
start codons at 35, 243, 246, 369, 462
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.947 = AGTAGTCAGGAATCGC-1

using 276 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2^2, 266]
surviving nonsolo ucounts = 1[266]
ids = [5]

====================================================================================

UMI info for barcode AGTAGTCAGGAATCGC-1 contig 1 = GAGGAATCAG...
umi GTACGTCAGT = 267 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.949 = AGTAGTCAGGACTGGT-1

using 281 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[4, 273]
surviving nonsolo ucounts = 1[273]
ids = [0]

====================================================================================

UMI info for barcode AGTAGTCAGGACTGGT-1 contig 1 = AGTGACTCCT...
umi AATTAACCAC = 258 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=542]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=3)
393-441 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
441-542 ==> 0-101 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 40 reads
cdr3 = CARRRSPWFGALDYW at 365, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 20, 64, 243, 246, 326, 335, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.951 = AGTAGTCAGGCATGGT-1

using 1422 reads

====================================================================================

graph has 1120 edges initially, 4 edges after simplification

total ucounts = 308
nonsolo ucounts = 128[2^56, 3^21, 4^25, 5^8, 6^5, 7^5, 8^2, 9^2, 10, 11, 12, 795]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.953 = AGTAGTCAGGCGACAT-1

using 289 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[287]
surviving nonsolo ucounts = 1[287]
ids = [2]

====================================================================================

UMI info for barcode AGTAGTCAGGCGACAT-1 contig 1 = GGGGCTTTCT...
umi TGCCTATGTC = 286 reads: +466 validated

GOOD CONTIGS

TIG 1[bases=585]
17-394 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=3)
394-415 ==> 0-21 on |21|IGHD3-3|D-REGION| [len=31] (mis=0)
420-483 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=4)
483-585 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CARGYYDFWSGLGIYYYYGMDVW at 383, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 282
start codons at 17, 26, 38, 82, 440, 501, 562
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.956 = AGTAGTCAGGGCTCTC-1

using 265 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[261]
surviving nonsolo ucounts = 1[261]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCAGGGCTCTC-1 contig 1 = GCTGCGGGTA...
umi GCTTAACCCC = 241 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=489]
0-40 ==> 0-40 on |318|IGLV1-36|5'UTR| [len=40] (mis=0)
40-393 ==> 0-353 on |319|IGLV1-36|L-REGION+V-REGION| [len=353] (mis=3)
390-428 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
428-489 ==> 0-61 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CAAWDDSLSGPVF at 361, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 40, 191, 248, 251, 344, 369, 374
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.958 = AGTAGTCAGTATCGAA-1

using 262 reads

====================================================================================

graph has 114 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[4, 257]
surviving nonsolo ucounts = 1[257]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCAGTATCGAA-1 contig 1 = GCTGGGGTCT...
umi TACCAGTACG = 253 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=580]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-395 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
429-580 ==> 0-151 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CSSHAGSTRVLF at 365, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 41, 198, 249, 348, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.969 = AGTAGTCCAAGCGCTC-1

using 485 reads

====================================================================================

graph has 172 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 182, 299]
surviving nonsolo ucounts = 2[182, 299]
ids = [0, 3]

====================================================================================

UMI info for barcode AGTAGTCCAAGCGCTC-1 contig 1 = AGCTTCAGCT...
umi TCTTATAGCG = 296 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=594]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-355 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-594 ==> 0-159 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CVAWDDSLYAWVF at 368, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 292
start codons at 47, 351, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.974 = AGTAGTCCAATAAGCA-1

using 22 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.980 = AGTAGTCCACTGCCAG-1

using 178 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[177]
surviving nonsolo ucounts = 1[177]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCCACTGCCAG-1 contig 1 = GAACCATGGA...
umi GACCAACGCA = 146 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=457]
5-350 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=2)
347-384 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
384-457 ==> 0-73 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CQQRSNWLTF at 326, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 141
start codons at 5, 210, 213, 426
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.982 = AGTAGTCCAGATAATG-1

using 1749 reads

====================================================================================

graph has 1638 edges initially, 20 edges after simplification

total ucounts = 545
nonsolo ucounts = 189[2^78, 3^35, 4^27, 5^15, 6^11, 7^11, 8^6, 9, 10^2, 18, 20, 691]
surviving nonsolo ucounts = 1[691]
ids = [456]

====================================================================================

REJECT CONTIGS

TIG 1[bases=376]
3-105 ==> 269-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=1)
143-194 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=4)
194-376 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
cdr3 = CARGHFYFASGSRIPGGVWFDPW at 94, score = 9 + 7
umis assigned: [456]
of which 1 are surviving nonsolos
reads assigned: 681
start codons at 
confident = false
VJ delta = 11
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.984 = AGTAGTCCAGATGGGT-1

using 408 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^3, 401]
surviving nonsolo ucounts = 1[401]
ids = [3]

====================================================================================

UMI info for barcode AGTAGTCCAGATGGGT-1 contig 1 = GAGGAATCAG...
umi GATTCGCTTT = 409 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 71 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 398
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.996 = AGTAGTCCATCGACGC-1

using 374 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 3[2, 7, 365]
surviving nonsolo ucounts = 1[365]
ids = [1]

====================================================================================

UMI info for barcode AGTAGTCCATCGACGC-1 contig 1 = AAGAGCTGCT...
umi CCACTCATGG = 362 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=553]
0-32 ==> 20-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
32-380 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
378-417 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 67 reads
cdr3 = CQQYGSSPYTF at 356, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 359
start codons at 32, 240, 366, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1005 = AGTAGTCCATTGCGGC-1

using 66589 reads

====================================================================================

graph has 13744 edges initially, 88 edges after simplification

total ucounts = 983
nonsolo ucounts = 512[2^109, 3^59, 4^30, 5^18, 6^12, 7^12, 8^6, 9^5, 10, 11^3, 13^2, 15^2, 16^2, 17, 19^2, 20^2, 21^2, 25^2, 33, 34, 35, 40, 44, 45, 48^2, 51, 55, 57^3, 58, 63, 68^2, 70, 71, 72, 76, 77^2, 78, 86, 88, 98, 99, 100, 104, 105, 108, 113, 115^2, 119, 121, 124, 125, 130, 132, 134, 138^2, 142, 144, 145, 148, 151^2, 152, 153, 157, 164, 167, 170, 171, 175^2, 178^2, 186, 187, 189, 196, 198, 204, 205, 208, 213^2, 218, 224, 225, 231, 232^3, 234^2, 237^2, 241^2, 244^2, 246, 248, 252^2, 253, 256, 257, 263, 264^2, 266, 267, 270, 271, 272, 275^3, 277, 281, 283, 284, 287^2, 288^3, 289, 291, 292^2, 293, 295, 296, 297^3, 299, 300, 301, 302^2, 303^3, 304, 305, 307, 309, 312^4, 313^2, 314^4, 315^2, 317^4, 318, 320^2, 321^2, 322^2, 324^2, 327^2, 329, 332, 334^3, 335^3, 336^3, 337, 338^3, 339^2, 340^2, 341, 342^2, 343^2, 345, 347, 351, 353^2, 355, 357, 358, 359, 362^2, 363^2, 367, 368^3, 370, 371, 372, 373, 375, 377^4, 378, 381^3, 382, 383, 384^2, 385, 389^2, 391^2, 393, 396, 407, 414, 420, 430, 431, 434, 435, 437, 445, 461, 473, 488, 650, 663, 665]
surviving nonsolo ucounts = 235[19, 20, 21, 25^2, 33, 34, 40, 44, 45, 48, 51, 55, 57^2, 63, 68, 71, 72, 77, 78, 86, 88, 98, 99, 100, 105, 113, 115^2, 121, 125, 130, 132, 134, 138^2, 142, 144, 145, 148, 151^2, 152, 153, 157, 164, 167, 170, 171, 175^2, 178^2, 186, 187, 189, 196, 198, 204, 205, 208, 213^2, 218, 224, 225, 231, 232^3, 234^2, 237^2, 241^2, 244^2, 246, 248, 252^2, 253, 256, 257, 263, 264^2, 266, 267, 270, 271, 272, 275^3, 277, 281, 283, 284, 287^2, 288^3, 289, 291, 292^2, 293, 295, 296, 297^3, 299, 300, 301, 302^2, 303^3, 304, 305, 307, 309, 312^4, 313^2, 314^4, 315^2, 317^4, 318, 320^2, 321^2, 322^2, 324^2, 327^2, 329, 332, 334^3, 335^3, 336^3, 337, 338^3, 339^2, 340^2, 341, 342^2, 343^2, 345, 347, 351, 353^2, 355, 357, 358, 359, 362^2, 363^2, 367, 368^3, 370, 371, 372, 373, 375, 377^4, 378, 381^3, 382, 383, 384^2, 385, 389^2, 391^2, 393, 396, 407, 414, 420, 430, 431, 434, 435, 437, 445, 461, 473, 488, 650, 663, 665]
ids = [961, 699, 186, 223, 858, 76, 198, 637, 274, 681, ...]

====================================================================================

UMI info for barcode AGTAGTCCATTGCGGC-1 contig 1 = TGGGGAGGAG...
umi AAACATGTTC = 356 reads: +388 validated
umi AAAGCCACGC = 355 reads: +388 validated
umi AAAGCCATGC = 203 reads: +388 validated
umi AACACATAGT = 377 reads: +388 validated
umi AACAGATACA = 338 reads: +388 validated
umi AACATCCAAC = 375 reads: +388 validated
umi AACCCTGATA = 290 reads: +158 -3XX +1 -1XX +1 -2XX +1 -5XX +1 -2XX +1 -6XX +1 -82X +2 -1XX +1 -4XX +1 -10XX +2 -4XX +98 invalidated
umi AACCGCCGAC = 288 reads: +388 validated
umi AACTTGCACG = 490 reads: -236 +152 non-validated
umi AAGGAGCGCC = 268 reads: +388 validated
umi AATAACGTAA = 325 reads: +388 validated
umi AATACAAGGA = 383 reads: +388 validated
umi AATGAGAGGG = 335 reads: +388 validated
umi AATTAAAACG = 288 reads: +388 validated
umi AATTGAAGGC = 666 reads: +388 validated
umi AATTGTGTGC = 270 reads: +388 validated
umi ACAGGTTCTC = 324 reads: +388 validated
umi ACATACCCAA = 344 reads: +388 validated
umi ACATAGGGGT = 460 reads: +47 -2X +2 -2XX +105 -6XX +1 -2XX +1 -4XX +2 -6XX +1 -98XX +1 -1XX +2 -2XX +5 -3XX +95 invalidated
umi ACCAAAGTCT = 325 reads: +388 validated
umi ACCCAATCAG = 322 reads: +388 validated
umi ACCGATGCAC = 435 reads: +388 validated
umi ACCGTCATGA = 288 reads: +388 validated
umi ACCTGATTTT = 318 reads: +388 validated
umi ACGGCACCTT = 336 reads: +388 validated
umi ACGTCGCCTA = 338 reads: +388 validated
umi ACTCCCCATC = 386 reads: +388 validated
umi AGAGAATGGG = 150 reads: +388 validated
umi AGAGGTCGTA = 212 reads: +388 validated
umi AGCCCTACAT = 253 reads: +388 validated
umi AGCCTGTCTC = 334 reads: +388 validated
umi AGCGGCTTGA = 366 reads: +388 validated
umi AGGAAGCCAG = 340 reads: +388 validated
umi AGGTCGCCCG = 288 reads: +388 validated
umi AGTACTCTTT = 331 reads: +89 -1 +1 -1XX +1 -2XX +1 -1XX +291 invalidated
umi AGTAGCATAT = 335 reads: +388 validated
umi ATAAATGAGG = 172 reads: +388 validated
umi ATACCACGTT = 691 reads: +159 -1XX +1 -1XX +1 -5XX +2 -13XX +1 -176XX +1 -2X +1 -2XX +3 -14XX +1 -2XX +1 -1XX invalidated
umi ATCACAAGGT = 323 reads: +388 validated
umi ATCGGAACCT = 321 reads: +388 validated
umi ATGACATAAC = 362 reads: +388 validated
umi ATGCGGCACA = 345 reads: +388 validated
umi ATGGTATTCC = 304 reads: +388 validated
umi ATGTGAGGCT = 304 reads: +388 validated
umi ATGTTGACAT = 299 reads: +388 validated
umi ATTCTACAGC = 394 reads: +388 validated
umi ATTGTCCAAC = 185 reads: +388 validated
umi ATTTATCGTA = 377 reads: +388 validated
umi CAAAATGCCA = 359 reads: +388 validated
umi CAAGCTGTGT = 310 reads: +388 validated
umi CACATCACTC = 323 reads: +388 validated
umi CACGTGTGGG = 333 reads: +388 validated
umi CAGGCGGCCC = 342 reads: +388 validated
umi CAGGTCGTGA = 315 reads: +388 validated
umi CATATCGTGC = 335 reads: +388 validated
umi CATCATCCCA = 377 reads: +388 validated
umi CATCTCACAA = 292 reads: +388 validated
umi CATGTAATGT = 382 reads: +388 validated
umi CATTACGCGG = 223 reads: -15 +373 non-validated
umi CATTACTAGC = 414 reads: +388 validated
umi CATTCACGCT = 226 reads: +388 validated
umi CATTCAGTGC = 271 reads: +388 validated
umi CATTGCGGAT = 139 reads: +388 validated
umi CATTTACCAA = 349 reads: +388 validated
umi CCACCTCACG = 231 reads: +388 validated
umi CCCAGGCCAC = 330 reads: +388 validated
umi CCCCATTTCG = 316 reads: +388 validated
umi CCCTAATTGC = 350 reads: +159 -1XX +1 -1XX +1 -5XX +2 -13XX +1 -179X +1 -2XX +3 -14XX +1 -2XX +1 -1XX invalidated
umi CCCTGTCTGG = 328 reads: +388 validated
umi CCGATGTTCC = 382 reads: +388 validated
umi CCGCATTTGT = 346 reads: +388 validated
umi CCGCCACCTC = 245 reads: +388 validated
umi CCTAAATTTA = 303 reads: +388 validated
umi CGAACTAGCG = 315 reads: +388 validated
umi CGACATAATA = 337 reads: +22 -1XX +365 invalidated
umi CGCTCCACTC = 320 reads: +388 validated
umi CGGGTGTTTG = 440 reads: +388 validated
umi CGGTGCGTAC = 264 reads: +388 validated
umi CGGTTACTAG = 336 reads: +388 validated
umi CTAAGTCACC = 388 reads: +388 validated
umi CTCACACCAC = 316 reads: +388 validated
umi CTCCGTAATA = 385 reads: +388 validated
umi CTCGAGCTAG = 367 reads: +388 validated
umi CTGAAAGTTG = 252 reads: +388 validated
umi CTGGACTTAC = 337 reads: +388 validated
umi CTGTATGCTG = 4 reads: -357X +6 -2X +15 -1XX +1 -1XX +5 invalidated
umi CTGTTCGTCA = 367 reads: +388 validated
umi CTTATCATGG = 309 reads: +388 validated
umi CTTCACGCCC = 305 reads: +388 validated
umi GACGGTCCCG = 265 reads: +388 validated
umi GAGTACTCCC = 282 reads: +388 validated
umi GATCATCTTC = 245 reads: +388 validated
umi GATTTAGGGT = 383 reads: +388 validated
umi GATTTGTCTG = 394 reads: +388 validated
umi GCAAGAATGG = 378 reads: +388 validated
umi GCACAAAGGC = 339 reads: +388 validated
umi GCACATTGTT = 240 reads: +388 validated
umi GCAGATAAGG = 377 reads: +388 validated
umi GCATCACATA = 322 reads: +388 validated
umi GCCACTCCTG = 367 reads: +388 validated
umi GCCTAGGGAT = 365 reads: +388 validated
umi GCCTAGTCAT = 395 reads: +388 validated
umi GCTAAGGTGG = 376 reads: +388 validated
umi GCTGTCCTCT = 455 reads: +158 -3XX +1 -1XX +1 -2XX +1 -5XX +1 -2XX +1 -6XX +1 -83 +1 -1X +1 -4XX +1 -10XX +2 -4XX +98 invalidated
umi GGACACTCTA = 303 reads: +388 validated
umi GGGCAACCCA = 286 reads: +388 validated
umi GGGTATACGT = 294 reads: +388 validated
umi GGTGCGAAGG = 240 reads: +388 validated
umi GTAACACCTC = 312 reads: +388 validated
umi GTAAGTTGTC = 270 reads: +388 validated
umi GTAATTGACT = 286 reads: +388 validated
umi GTACTCATGA = 2 reads: -348 +1 -5X +2 -1X +6 -2X +15 -1XX +1 -1XX +5 invalidated
umi GTAGTACCAG = 299 reads: +388 validated
umi GTATTTACTT = 318 reads: +388 validated
umi GTCACAACGA = 236 reads: +388 validated
umi GTCAGGTATG = 329 reads: +388 validated
umi GTCATTGGAG = 349 reads: +388 validated
umi GTCCAACGAC = 188 reads: +388 validated
umi GTCCGTACCC = 2 reads: -354X +2 -1X +6 -2X +15 -1XX +1 -1XX +5 invalidated
umi GTGAAGGTTT = 310 reads: +388 validated
umi GTGCGCTCCC = 434 reads: +388 validated
umi GTGTCTTCCG = 222 reads: +388 validated
umi GTTCTCTCAT = 300 reads: +388 validated
umi GTTTCACTCT = 307 reads: +348 -1XX +2 -37XX invalidated
umi TAACATGAGA = 300 reads: +388 validated
umi TAACTTGCTC = 244 reads: +388 validated
umi TACAGAAATT = 240 reads: +388 validated
umi TACATAAGGC = 374 reads: +388 validated
umi TACTTCACAA = 204 reads: +388 validated
umi TAGCAGGAGG = 257 reads: +388 validated
umi TATATTTCTT = 191 reads: -341X +47 invalidated
umi TATCTAGGGA = 305 reads: +388 validated
umi TATCTATGTT = 316 reads: +388 validated
umi TATTGAGGCT = 368 reads: +388 validated
umi TATTGCACCG = 325 reads: +388 validated
umi TCACGGTCCT = 299 reads: +388 validated
umi TCCATCTCGG = 315 reads: +388 validated
umi TCCATTCGGC = 289 reads: +388 validated
umi TCCCAACGAT = 313 reads: +388 validated
umi TCCGACATCG = 294 reads: +388 validated
umi TCCGCAGTAC = 370 reads: +388 validated
umi TCCGCTTGTA = 340 reads: +388 validated
umi TCCTTGGCGA = 180 reads: +388 validated
umi TCGAATTCGC = 179 reads: +388 validated
umi TCTCGATTAC = 297 reads: +388 validated
umi TCTTCAAACA = 295 reads: +388 validated
umi TGACGGTCTA = 236 reads: +388 validated
umi TGAGTTGGGC = 350 reads: +388 validated
umi TGATAAATGG = 456 reads: +388 validated
umi TGCATTGTGC = 271 reads: +388 validated
umi TGCCGGGTGG = 146 reads: +388 validated
umi TGGTACCTTC = 411 reads: +388 validated
umi TGTAACTATC = 190 reads: +388 validated
umi TGTGCCTGAC = 391 reads: +388 validated
umi TGTGGGACCT = 270 reads: +388 validated
umi TGTTACAGAG = 123 reads: +388 validated
umi TTAATAGGTC = 296 reads: +388 validated
umi TTAATGGAAT = 338 reads: +388 validated
umi TTAATTCTCT = 359 reads: +388 validated
umi TTATACGTGA = 505 reads: +158 -3XX +1 -1XX +1 -2XX +1 -5XX +1 -2XX +1 -6XX +1 -82X +2 -1XX +1 -4XX +1 -10XX +2 -4XX +98 invalidated
umi TTATCTCATC = 363 reads: +388 validated
umi TTCACAACGG = 272 reads: +388 validated
umi TTCACCGTTC = 363 reads: +388 validated
umi TTCAGCAGGA = 208 reads: +388 validated
umi TTCAGGAGGG = 329 reads: +388 validated
umi TTCTATCCAG = 336 reads: +388 validated
umi TTGATCTAAC = 276 reads: +388 validated
umi TTGCACTTTG = 166 reads: +388 validated
umi TTGGAGCCCT = 310 reads: +388 validated
umi TTGGCGAAGC = 344 reads: +388 validated
umi TTGTATGCGT = 356 reads: +388 validated
umi TTTCGGGCCC = 398 reads: +388 validated
umi TTTCTTTATA = 241 reads: +388 validated
umi TTTGCACCAT = 690 reads: +158 -3XX +1 -1XX +1 -2XX +1 -5XX +1 -2XX +1 -6XX +1 -82X +2 -1XX +1 -4XX +1 -10XX +2 -4XX +98 invalidated

UMI info for barcode AGTAGTCCATTGCGGC-1 contig 2 = AGTGCTTTCT...
umi AACGATTACC = 153 reads: +436 validated
umi AAGCGGTTCG = 116 reads: +436 validated
umi ACAGGCTCCC = 33 reads: -1 +368 -1 +11 -44 +5 -1 +5 non-validated
umi ACGTGGGTGA = 73 reads: +436 validated
umi ACTTTCTGTG = 53 reads: +347 -2 +13 -1 +61 -12 non-validated
umi AGGACCGACT = 47 reads: +402 -1 +33 non-validated
umi AGGCTAGGTA = 60 reads: +284 -10 +142 non-validated
umi AGTCAACCGC = 21 reads: +37 -43 +356 non-validated
umi ATAACAGCTG = 35 reads: +81 -1 +14 -3 +337 non-validated
umi ATCAGGTGTC = 166 reads: +436 validated
umi ATCTCGCGGA = 307 reads: +436 validated
umi ATTTTTGTTC = 44 reads: -24 +338 -1 +1 -1 +69 -1 +1 non-validated
umi CAATCATATA = 213 reads: +436 validated
umi CAGAATATCC = 131 reads: +436 validated
umi CCATGACCTC = 180 reads: +436 validated
umi CCGCAGTTCA = 171 reads: +436 validated
umi CCTATTCCAG = 70 reads: -10 +426 non-validated
umi CTAATCGTTA = 68 reads: +436 validated
umi CTCCATTCGT = 157 reads: +436 validated
umi CTGATAGCCA = 142 reads: +436 validated
umi GCATAGGGAT = 252 reads: +436 validated
umi GCTTCGATAG = 122 reads: +436 validated
umi GGATGCGGGC = 85 reads: -16 +420 non-validated
umi GGCCAAGTCA = 58 reads: -12 +424 non-validated
umi GGCGAAAGCG = 177 reads: +436 validated
umi GGTGATACCG = 251 reads: +436 validated
umi GGTTGTATGC = 102 reads: +436 validated
umi GTAATATAAG = 40 reads: +436 validated
umi GTGACCTAGA = 252 reads: +436 validated
umi GTTTTAGCAG = 19 reads: +23 -17 +21 -1 +6 -1XX +1 -2X +3 -1 +3 -1 +187 -38 +7 -3 +5 -1 +4 -1 +5 -1 +1 -2 +2 -1 +9 -1 +5 -1 +2 -1 +1 -78 invalidated
umi TAAGTATGGG = 99 reads: +423 -2 +11 non-validated
umi TACATAATCC = 295 reads: +436 validated
umi TACGGTTCAC = 152 reads: +436 validated
umi TATTCATCAG = 130 reads: +436 validated
umi TCCGGACATT = 152 reads: +436 validated
umi TCCGGTCTCT = 78 reads: +421 -12 +3 non-validated
umi TCCTACGCCC = 231 reads: +436 validated
umi TCTCACAGGA = 111 reads: +436 validated
umi TCTCGCCTCC = 209 reads: +436 validated
umi TGATTGGTAT = 21 reads: -15 +360 -9 +37 -1 +14 non-validated
umi TTCGGGCTCA = 50 reads: +399 -37 non-validated
umi TTCTTTGCTG = 116 reads: +436 validated
umi TTGGTCATTC = 19 reads: -45 +235 -18 +7 -1 +2 -1 +8 -1 +36 -28 +54 non-validated

GOOD CONTIGS

TIG 1[bases=556]
32-385 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=13)
383-420 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 162 umis using 8215 reads
cdr3 = CQQSYSTLLTF at 359, score = 9 + 8
umis assigned: [5, 10, 11, 19, 21, 23, 27, 29, 36, 42] and 164 others
of which 174 are surviving nonsolos
reads assigned: 53766
start codons at 32, 38, 94, 107, 243, 462
confident = true

TIG 2[bases=635]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=8)
405-453 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
453-635 ==> 0-182 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
junction support: 36 umis using 661 reads
cdr3 = CARAHGDYYTLLDCW at 377, score = 8 + 7
umis assigned: [32, 39, 76, 120, 137, 163, 169, 186, 198, 213] and 33 others
of which 43 are surviving nonsolos
reads assigned: 5189
start codons at 17, 38, 82, 168, 368
confident = true

REJECT CONTIGS

TIG 1[bases=864]
2-589 ==> 2058-2645 on rc of segment before IGHD1-1 exon 1 [len=2645] (mis=0)
619-682 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=2)
682-864 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
umis assigned: [223, 296, 400, 457, 458, 681, 835, 861, 944]
of which 9 are surviving nonsolos
reads assigned: 1214
start codons at 2, 10, 639
confident = false
did not find CDR3
now this is a cell
paired!

GCCGCGGACACGGCTATGTATTACTGTGCGAGAGCACACGGTGACTACTACACCCTCCTTGACTGCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGCACCCTCCTCACTTTCGGCGGAGGGACCAAGGTGGAGACCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1008 = AGTAGTCGTAAACACA-1

using 485 reads

====================================================================================

graph has 184 edges initially, 12 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 229, 249]
surviving nonsolo ucounts = 2[229, 249]
ids = [0, 5]

====================================================================================

UMI info for barcode AGTAGTCGTAAACACA-1 contig 1 = CTGGGCCTCA...
umi CTCTATCACG = 238 reads: +379 validated

UMI info for barcode AGTAGTCGTAAACACA-1 contig 2 = TGAGCGCAGA...
umi AAGACCGGTC = 224 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=568]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-371 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=12)
378-416 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
416-568 ==> 0-152 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQAWDSTTDWVF at 352, score = 6 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 233
start codons at 37, 42, 98, 185, 331, 335
confident = false

TIG 2[bases=576]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=8)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
424-576 ==> 0-152 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CGTWDRSLSAGVI at 357, score = 7 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 36, 241, 365
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1009 = AGTAGTCGTAACGACG-1

using 395 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[393]
surviving nonsolo ucounts = 1[393]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=330]
1-143 ==> 195-337 on |244|IGKV1D-13|L-REGION+V-REGION| [len=351] (mis=9)
34-157 ==> 228-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=7)
156-194 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
194-330 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNGQSRAF at 133, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 383
start codons at 17, 20, 113, 146, 236
confident = false
not full
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1012 = AGTAGTCGTAGAAAGG-1

using 429 reads

====================================================================================

graph has 206 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[429]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1013 = AGTAGTCGTAGATTAG-1

using 1311 reads

====================================================================================

graph has 518 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 14^2, 250, 320, 706]
surviving nonsolo ucounts = 3[14, 320, 706]
ids = [2, 1, 9]

====================================================================================

UMI info for barcode AGTAGTCGTAGATTAG-1 contig 1 = TGGGGAGGAA...
umi ACTAGGTAAT = 187 reads: +22 -1XX +25 -1XX +12 -2XX +26 -1XX +3 -1XX +16 -1XX +13 -22X +1 -3X +4 -2XX +1 -5XX +2 -1XX +1 -1XX +5 -1XX +8 -1XX +20 -1XX +3 -1XX +3 -1XX +2 -3XX +2 -1XX +2 -1XX +5 -1XX +30 -2XX +27 -1XX +20 -1XX +8 -1XX +11 -4XX +2 -2XX +1 -1XX +1 -1XX +2 -1XX +1 -6XX +8 -1XX +28 invalidated
umi AGCACGGCAC = 318 reads: +388 validated
umi ATCTGAAATC = 14 reads: +32 -8 +215 -1 +2 -1 +10 -19 +56 -30 +2 -2 +10 non-validated
umi TAGTGTTAGG = 715 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=556]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 168 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [0, 1, 2, 9]
of which 3 are surviving nonsolos
reads assigned: 1181
start codons at 32, 38, 107, 243, 462
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1016 = AGTAGTCGTCAGCTAT-1

using 7851 reads

====================================================================================

graph has 3906 edges initially, 34 edges after simplification

total ucounts = 601
nonsolo ucounts = 255[2^93, 3^55, 4^29, 5^19, 6^15, 7^4, 8^7, 10, 12^4, 13, 15, 22, 53, 67, 123, 138, 152, 169, 186, 206, 229, 232, 242, 244, 247, 259, 260, 271, 280, 286, 316, 317, 319, 324, 542, 550, 649]
surviving nonsolo ucounts = 26[6, 53, 67, 123, 138, 152, 169, 186, 206, 229, 232, 242, 244, 247, 259, 260, 271, 280, 286, 316, 317, 319, 324, 542, 550, 649]
ids = [214, 436, 342, 365, 290, 292, 252, 36, 66, 86, ...]

====================================================================================

UMI info for barcode AGTAGTCGTCAGCTAT-1 contig 1 = GAGTCAGTCT...
umi CTAATTTTCT = 653 reads: +388 validated
umi GCAGCATATA = 282 reads: +388 validated
umi TCCGAGAGTA = 322 reads: +388 validated

UMI info for barcode AGTAGTCGTCAGCTAT-1 contig 2 = AGCTCTGGGA...
umi AAAGCGGGTC = 247 reads: +375 -13XX +1 -4X +1 -3 +36 invalidated
umi ACCTAGACTC = 185 reads: +433 validated
umi AGAAACGCTT = 266 reads: +433 validated
umi AGCAGAGCAA = 204 reads: +433 validated
umi ATACTAGCCT = 213 reads: +432 -1X invalidated
umi CAGAGCTTTT = 274 reads: +293 -1XX +125 -14 invalidated
umi CATCATTACC = 286 reads: +433 validated
umi CCATTATTCT = 229 reads: +423 -1X +9 invalidated
umi CGTAAATTCA = 170 reads: +401 -1 +1 -1 +10 -19 non-validated
umi CTCTGTCGTA = 135 reads: +392 -1 +2 -1 +37 non-validated
umi CTCTTAAATA = 155 reads: +433 validated
umi GAGTCGTTTA = 65 reads: +433 validated
umi GCACTCCCTA = 517 reads: +433 validated
umi GCGCTATAAG = 14 reads: -143X +4 -4XX +3 -4XX +3 -1XX +3 -1XX +8 -1XX +1 -1XX +21 -1XX +2 -4XX +1 -1XX +2 -3XX +1 -2XX +6 -1XX +2 -209XX invalidated
umi GCTAAGCCTC = 236 reads: +433 validated
umi GTCATAATAA = 316 reads: +422 -11 non-validated
umi TCCTCAAGCC = 306 reads: +433 validated
umi TTAGTTACCG = 246 reads: +433 validated
umi TTCACAGCTA = 274 reads: +413 -2 +18 non-validated

GOOD CONTIGS

TIG 1[bases=549]
25-378 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=5)
374-413 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 3 umis using 206 reads
cdr3 = CQQSYSTLWTF at 352, score = 9 + 8
umis assigned: [264, 356, 486]
of which 3 are surviving nonsolos
reads assigned: 1233
start codons at 25, 31, 87, 100, 236, 455
confident = true

TIG 2[bases=584]
0-80 ==> 40-120 on |105|IGHV3-11|5'UTR| [len=120] (mis=0)
80-433 ==> 0-353 on |106|IGHV3-11|L-REGION+V-REGION| [len=353] (mis=8)
450-513 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=5)
513-584 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 11 umis using 151 reads
cdr3 = CARGRKQLAGGYYYYGMDVW at 422, score = 9 + 7
umis assigned: [1, 36, 56, 66, 86, 151, 161, 184, 252, 290] and 9 others
of which 19 are surviving nonsolos
reads assigned: 4253
start codons at 80, 236, 383, 470
confident = true

REJECT CONTIGS

TIG 1[bases=558]
0-77 ==> 5533-5610 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=2)
30-390 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=2)
384-422 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
422-558 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [240, 436]
of which 2 are surviving nonsolos
reads assigned: 370
start codons at 30, 63, 91, 99, 187, 349, 369, 464
confident = false
did not find CDR3
now this is a cell
paired!

GTGTATTACTGTGCGAGAGGCAGGAAGCAGCTCGCCGGGGGGTACTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> ATCAGCAGTCTGCAACCTGAAGATTTTGCAACTTACTACTGTCAACAGAGTTACAGTACCTTGTGGACTTTTGGCCAGGGGACCAAGCTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1027 = AGTAGTCGTTCCCTTG-1

using 704 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[702]
surviving nonsolo ucounts = 1[702]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1035 = AGTAGTCTCATCGGAT-1

using 260 reads

====================================================================================

graph has 130 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[256]
surviving nonsolo ucounts = 1[256]
ids = [4]

====================================================================================

UMI info for barcode AGTAGTCTCATCGGAT-1 contig 1 = GAGTCTCCCT...
umi TACCAGACCT = 254 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=527]
0-58 ==> 1-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=0)
58-411 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=14)
426-476 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
476-527 ==> 0-51 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CARRYGDYVYAFDFW at 400, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 248
start codons at 58, 232, 256, 413, 428
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1045 = AGTAGTCTCCCTTGTG-1

using 450 reads

====================================================================================

graph has 148 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[445]
surviving nonsolo ucounts = 1[445]
ids = [3]

====================================================================================

UMI info for barcode AGTAGTCTCCCTTGTG-1 contig 1 = GGGGGACTCC...
umi TACTGGTCGG = 348 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=476]
21-379 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=4)
380-396 ==> 0-16 on |25|IGHD4-17|D-REGION| [len=16] (mis=1)
404-457 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=2)
457-476 ==> 0-19 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CAHIQDYGDYVFSNWYFDLW at 366, score = 7 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 344
start codons at 21, 65, 244, 247, 327, 336
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1051 = AGTAGTCTCCGTTGTC-1

using 1072 reads

====================================================================================

graph has 816 edges initially, 2 edges after simplification

total ucounts = 187
nonsolo ucounts = 84[2^36, 3^19, 4^10, 5^5, 6^4, 7^4, 9^3, 15, 16, 665]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1057 = AGTAGTCTCGATAGAA-1

using 1101 reads

====================================================================================

graph has 308 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 6, 7, 1079]
surviving nonsolo ucounts = 1[1079]
ids = [8]

====================================================================================

REJECT CONTIGS

TIG 1[bases=386]
4-137 ==> 223-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=3)
137-175 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
175-386 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 105, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 1059
start codons at 88, 115, 139, 307
confident = false
not full
VJ delta = 22
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1062 = AGTAGTCTCGGCCGAT-1

using 397 reads

====================================================================================

graph has 204 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[4^2, 174, 210]
surviving nonsolo ucounts = 2[174, 210]
ids = [3, 6]

====================================================================================

UMI info for barcode AGTAGTCTCGGCCGAT-1 contig 1 = AGCTCTGAGA...
umi CGTTAACTGC = 176 reads: +424 validated
umi TGATTCATAC = 209 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=562]
0-79 ==> 0-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
456-503 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
503-562 ==> 0-59 on |43|IGHG2|C-REGION| [len=977] (mis=0)
junction support: 2 umis using 33 reads
cdr3 = CARDRAATARLGGMDVW at 421, score = 9 + 7
umis assigned: [3, 6]
of which 2 are surviving nonsolos
reads assigned: 374
start codons at 79, 230, 235, 382, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1068 = AGTAGTCTCTAACCGA-1

using 984 reads

====================================================================================

graph has 1175 edges initially, 10 edges after simplification

total ucounts = 380
nonsolo ucounts = 149[2^54, 3^35, 4^20, 5^13, 6^4, 7^4, 8^6, 9^4, 10^2, 11^2, 12^2, 13, 17, 163]
surviving nonsolo ucounts = 1[163]
ids = [281]

====================================================================================

UMI info for barcode AGTAGTCTCTAACCGA-1 contig 1 = GAGGAATCAG...
umi TACTACCTTT = 163 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [281]
of which 1 are surviving nonsolos
reads assigned: 160
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1070 = AGTAGTCTCTCCCTGA-1

using 49 reads

====================================================================================

graph has 52 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 5[2, 9, 10, 12, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1072 = AGTAGTCTCTGATTCT-1

using 195 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 186]
surviving nonsolo ucounts = 1[186]
ids = [2]

====================================================================================

UMI info for barcode AGTAGTCTCTGATTCT-1 contig 1 = ATGGACACAC...
umi CACTTTACGG = 172 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=478]
0-358 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=20)
388-436 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
436-478 ==> 0-42 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CAHIVGFYFPNSGYYYFDSW at 345, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 170
start codons at 0, 44, 223, 226, 315
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1086 = AGTCTTTAGATCCTGT-1

using 230 reads

====================================================================================

graph has 340 edges initially, 2 edges after simplification

total ucounts = 134
nonsolo ucounts = 37[2^17, 3^6, 4^3, 5^4, 6^3, 7^2, 8, 9]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1098 = AGTCTTTAGCTGTCTA-1

using 56 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 5[2, 3, 5, 15, 25]
surviving nonsolo ucounts = 1[25]
ids = [8]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1102 = AGTCTTTAGGATGGTC-1

using 323 reads

====================================================================================

graph has 124 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 116, 197]
surviving nonsolo ucounts = 2[116, 197]
ids = [1, 3]

====================================================================================

UMI info for barcode AGTCTTTAGGATGGTC-1 contig 1 = GTCAGACTCA...
umi CGTGTCCGCA = 190 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
411-492 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQQYNGQSRAF at 350, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 187
start codons at 23, 29, 98, 234, 237, 330, 363, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1105 = AGTCTTTAGGGAACGG-1

using 1528 reads

====================================================================================

graph has 1968 edges initially, 22 edges after simplification

total ucounts = 611
nonsolo ucounts = 304[2^112, 3^78, 4^29, 5^33, 6^13, 7^9, 8^6, 9^4, 10^6, 11^2, 12^5, 13^2, 15, 17, 18^2, 21]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1108 = AGTCTTTAGTAAGTAC-1

using 12 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1118 = AGTCTTTAGTTAGCGG-1

using 16791 reads

====================================================================================

graph has 6834 edges initially, 74 edges after simplification

total ucounts = 1047
nonsolo ucounts = 526[2^191, 3^107, 4^66, 5^33, 6^26, 7^10, 8^8, 9^5, 10^4, 11^4, 12, 13^2, 14^2, 15, 40, 79, 93, 113, 114, 121, 122, 124, 144^2, 151, 153, 155, 156, 157, 164, 165, 166, 171, 173, 174, 175, 178, 180, 185, 187, 188^2, 190, 207^2, 210, 211, 213, 216, 218, 220, 222, 225, 230, 231, 236^2, 237, 241, 242, 245^2, 247, 251, 256, 257, 259, 268, 274, 280, 289, 295, 313, 332, 346, 362, 429, 450, 478, 609]
surviving nonsolo ucounts = 64[13, 15, 93, 113, 114, 122, 124, 144^2, 151, 153, 155, 156, 157, 164, 165, 171, 173, 174, 175, 178, 180, 185, 187, 188^2, 190, 207^2, 210, 211, 213, 216, 218, 220, 222, 225, 230, 231, 236^2, 237, 241, 242, 245^2, 247, 251, 256, 257, 259, 268, 274, 280, 289, 295, 313, 332, 346, 362, 429, 450, 478, 609]
ids = [751, 349, 731, 320, 973, 800, 206, 462, 804, 868, ...]

====================================================================================

UMI info for barcode AGTCTTTAGTTAGCGG-1 contig 1 = AGCATCATCC...
umi AAATTGTACC = 284 reads: +445 validated
umi AAGCGCAGGC = 46 reads: +18 -1XX +2 -1XX +3 -1XX +4 -1XX +4 -1XX +1 -1XX +3 -2XX +3 -2XX +2 -1X +1 -17 +1 -2XX +9 -1XX +4 -2XX +14 -1XX +25 -1XX +4 -1XX +1 -1XX +11 -3XX +1 -1XX +4 -1XX +1 -1XX +26 -1XX +18 -3XX +10 -228X invalidated
umi CCCCTCGCCC = 14 reads: +109 -78 +217 -41 non-validated
umi GCGATGCCAT = 202 reads: +445 validated
umi TATATAAGGG = 13 reads: +72 -2 +2 -1 +75 -51 +64 -29 +6 -1 +66 -3 +56 -17 non-validated
umi TCCATACGGT = 121 reads: +445 validated
umi TGCTCACACT = 161 reads: +445 validated

UMI info for barcode AGTCTTTAGTTAGCGG-1 contig 2 = AGCTTCAGCT...
umi AACGAATGGG = 321 reads: +312 -1XX +1 -5XX +1 -3XX +3 -1XX +2 -1XX +1 -1XX +2 -30XX +1 -1X +3 -2XX +1 -9XX +1 -4XX +2 invalidated
umi AAGATGCCGT = 241 reads: +22 -1XX +7 -13XX +1 -2XX +2 -9X +1 -1XX +1 -1XX +2 -3XX +2 -2XX +1 -4XX +313 invalidated
umi AATATTACCC = 214 reads: +388 validated
umi AGAATCGTAG = 257 reads: +388 validated
umi AGACATCCTT = 306 reads: +312 -1XX +1 -5XX +1 -3XX +3 -1XX +2 -1XX +1 -1XX +2 -28XX +1 -1XX +1 -1XX +3 -2XX +1 -9XX +1 -4XX +2 invalidated
umi AGCCTTCGGT = 202 reads: +388 validated
umi AGTAGCCAGT = 238 reads: +388 validated
umi AGTCGCTATA = 11 reads: -388 non-validated
umi ATCACATCCT = 14 reads: -387X +1 invalidated
umi ATCATTCTTC = 221 reads: +388 validated
umi ATCGTCTCGC = 209 reads: +388 validated
umi ATCTGTTCTA = 170 reads: +388 validated
umi ATTATCATCC = 224 reads: +388 validated
umi CAAGTCCTTA = 154 reads: +388 validated
umi CATAGTCCTG = 177 reads: +312 -1XX +1 -5XX +1 -3XX +3 -1XX +2 -1X +1 -1X +2 -30X +1 -1X +3 -2X +1 -9X +1 -4XX +2 invalidated
umi CATCTAAACA = 142 reads: +380 -8 non-validated
umi CATTAACTCA = 20 reads: -381X +1 -5XX +1 invalidated
umi CCACCATCGA = 276 reads: +388 validated
umi CCACGAGGTA = 165 reads: +309 -67 +3 -1 +1 -3X +2 -1 +1 invalidated
umi CCGGCTCAAG = 152 reads: +388 validated
umi CCGGTTAGTT = 251 reads: +388 validated
umi CCGTTACCTT = 153 reads: +388 validated
umi CGAGACGTGG = 229 reads: +388 validated
umi CGGTTCAGTT = 173 reads: +388 validated
umi CGTAAAGTAG = 186 reads: +388 validated
umi CTCCGGTCTT = 238 reads: +388 validated
umi CTCTTATGTC = 141 reads: +388 validated
umi CTGACCACAC = 191 reads: +388 validated
umi CTTCTATCTG = 187 reads: +388 validated
umi CTTGCGACTC = 220 reads: +388 validated
umi GAAAGTATAG = 214 reads: +388 validated
umi GAACATCTGA = 463 reads: +388 validated
umi GATCCTATTA = 278 reads: +388 validated
umi GATGGTGCCC = 10 reads: -381X +1 -5X +1 invalidated
umi GCAAAATGGC = 184 reads: +388 validated
umi GCCAAGCGGC = 257 reads: +388 validated
umi GGCAAAGCCT = 268 reads: +388 validated
umi GGTCCATTTG = 15 reads: -381X +1 -5XX +1 invalidated
umi GGTTATCATT = 355 reads: +312 -1XX +1 -5XX +1 -3XX +3 -1XX +2 -1XX +1 -1XX +2 -28 +1 -1X +1 -1X +3 -2XX +1 -9XX +1 -4XX +2 invalidated
umi GTACGTGTAA = 491 reads: +120 -1XX +267 invalidated
umi GTTAATTCCC = 237 reads: +388 validated
umi TAAATTAGGG = 214 reads: +388 validated
umi TAACACGGAC = 180 reads: +388 validated
umi TAGACACAAT = 94 reads: +388 validated
umi TCACTGGGGA = 15 reads: -359X +2 -4XX +2 -4XX +1 -2XX +1 -6XX +1 -5XX +1 invalidated
umi TCCCGTCGCC = 146 reads: +388 validated
umi TCCCTCATCC = 222 reads: +388 validated
umi TCCTGTATTG = 18 reads: -387X +1 invalidated
umi TCCTTCCTCT = 15 reads: -359X +2 -4X +2 -4XX +1 -2XX +1 -6XX +1 -5XX +1 invalidated
umi TGACAGATGT = 152 reads: +388 validated
umi TGACTCTACT = 241 reads: +388 validated
umi TGCCAGACCT = 428 reads: +388 validated
umi TGGAAGTGAC = 171 reads: +388 validated
umi TTCGTCTGGG = 16 reads: -360 +1 -4X +2 -4XX +1 -2XX +1 -6XX +1 -5XX +1 invalidated
umi TTTCAAACCG = 247 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=585]
0-61 ==> 243-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=2)
61-414 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=38)
443-506 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=5)
506-585 ==> 0-79 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 4 umis using 68 reads
cdr3 = CVRGYCGRSCSRGLNYYYYPMDVW at 403, score = 9 + 7
umis assigned: [13, 41, 349, 574, 751, 800, 894]
of which 6 are surviving nonsolos
reads assigned: 829
start codons at 61, 148, 259, 325, 358, 463, 560
confident = true

TIG 2[bases=645]
0-46 ==> 10-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
46-397 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=17)
396-434 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
434-645 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 40 umis using 1282 reads
cdr3 = CAPWDDSLSGWVF at 367, score = 7 + 8
umis assigned: [23, 36, 52, 131, 133, 151, 166, 174, 206, 209] and 45 others
of which 55 are surviving nonsolos
reads assigned: 10399
start codons at 46, 197, 200, 350, 380
confident = true

REJECT CONTIGS

TIG 1[bases=422]
1-167 ==> 976-1142 on rc of segment before IGHD3-22 exon 1 [len=1142] (mis=0)
189-240 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=2)
240-422 ==> 0-182 on |45|IGHG3|C-REGION| [len=1130] (mis=1)
umis assigned: [243, 280, 558, 750]
of which 3 are surviving nonsolos
reads assigned: 1066
start codons at 18, 77
confident = false
did not find CDR3
now this is a cell
paired!

GTTAGGGGATATTGTGGTAGAAGTTGCTCCCGGGGACTGAACTACTACTACTACCCCATGGACGTCTGGGGCAAAGGGACCACGGTCACCGTCTCCTCAG <==> GGGCTCCGGTCCGAGGATGAGGGTAATTATTACTGTGCACCTTGGGATGACAGCCTGAGTGGCTGGGTGTTCGGCGGAGGGACCAAACTGACCGTCCTGG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1123 = AGTCTTTCAAAGTCAA-1

using 342 reads

====================================================================================

graph has 138 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[94, 244]
surviving nonsolo ucounts = 2[94, 244]
ids = [1, 0]

====================================================================================

UMI info for barcode AGTCTTTCAAAGTCAA-1 contig 1 = GATCAGGACT...
umi AGCTTGTGCG = 238 reads: +397 validated

UMI info for barcode AGTCTTTCAAAGTCAA-1 contig 2 = GGGGGGGGTC...
umi ATAAGGTCCA = 93 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=525]
0-30 ==> 0-30 on |274|IGKV2D-29|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=1)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
427-525 ==> 0-98 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CMQSIQLPLTF at 366, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 234
start codons at 30, 63, 99, 187, 250, 349, 369, 469
confident = false

TIG 2[bases=476]
0-42 ==> 0-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=3)
42-370 ==> 0-328 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=14)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
430-476 ==> 0-46 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CSSEAGSYTLLF at 366, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 92
start codons at 42, 193, 199, 250, 253, 349
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1128 = AGTCTTTCAAGTCTAC-1

using 274 reads

====================================================================================

graph has 113 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 270]
surviving nonsolo ucounts = 1[270]
ids = [2]

====================================================================================

UMI info for barcode AGTCTTTCAAGTCTAC-1 contig 1 = GGAGTCAGTC...
umi CTATTTGCGT = 252 reads: +298 -1XX +89 invalidated

GOOD CONTIGS

TIG 1[bases=517]
0-26 ==> 32-58 on |238|IGKV1-9|5'UTR| [len=58] (mis=0)
26-377 ==> 0-351 on |239|IGKV1-9|L-REGION+V-REGION| [len=351] (mis=11)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-517 ==> 0-103 on |212|IGKC|C-REGION| [len=320] (mis=2)
junction support: 1 umis using 31 reads
cdr3 = CQHISTYPWTF at 353, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 250
start codons at 26, 32, 88, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1139 = AGTCTTTCACCTGGTG-1

using 473 reads

====================================================================================

graph has 186 edges initially, 24 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[232, 239]
surviving nonsolo ucounts = 2[232, 239]
ids = [2, 1]

====================================================================================

UMI info for barcode AGTCTTTCACCTGGTG-1 contig 1 = AGCTTCAGCT...
umi CTCTCTCCGT = 240 reads: +385 validated

UMI info for barcode AGTCTTTCACCTGGTG-1 contig 2 = GGGGTCACAA...
umi GACGCTCTGA = 224 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=565]
0-46 ==> 10-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
46-397 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=4)
393-431 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
431-565 ==> 0-134 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 29 reads
cdr3 = CAAWDDSLGGVF at 367, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 237
start codons at 46, 200, 350, 375, 380
confident = false

TIG 2[bases=557]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=3)
385-423 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
423-557 ==> 0-134 on |306|IGLC2|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CCSYAGSLWVF at 362, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 38, 177, 239, 246, 372, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1141 = AGTCTTTCACGGCGTT-1

using 598 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[7, 586]
surviving nonsolo ucounts = 1[586]
ids = [3]

====================================================================================

UMI info for barcode AGTCTTTCACGGCGTT-1 contig 1 = GATCAGGACT...
umi CTCACCTCCC = 583 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=563]
0-30 ==> 0-30 on |264|IGKV2-28|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=3)
389-427 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=2)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 89 reads
cdr3 = CMQALQTKVTF at 366, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 578
start codons at 30, 63, 99, 187, 349, 369, 469
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1152 = AGTCTTTCAGGGTACA-1

using 315 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 310]
surviving nonsolo ucounts = 1[310]
ids = [0]

====================================================================================

UMI info for barcode AGTCTTTCAGGGTACA-1 contig 1 = GTCAGACCCT...
umi ACTGAAGGGG = 311 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=547]
0-29 ==> 24-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
29-374 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
383-411 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYYSYPRTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 307
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1155 = AGTCTTTCATCACAAC-1

using 269 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[269]
surviving nonsolo ucounts = 1[269]
ids = [0]

====================================================================================

UMI info for barcode AGTCTTTCATCACAAC-1 contig 1 = GTCAGTCTCA...
umi CCTCGTCTTC = 251 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=499]
0-23 ==> 24-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
23-362 ==> 0-339 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=13)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=5)
414-499 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQSDDKSPITF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 23, 29, 85, 98, 234, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1157 = AGTCTTTCATCACGTA-1

using 11587 reads

====================================================================================

graph has 5048 edges initially, 64 edges after simplification

total ucounts = 807
nonsolo ucounts = 393[2^140, 3^76, 4^59, 5^23, 6^21, 7^11, 8^3, 9^4, 10, 11, 12^2, 14, 15, 18, 19, 22, 28, 29, 48, 51, 56, 60, 69, 107, 123, 129, 130, 137, 152, 163, 167, 174, 181, 183, 187, 191, 200^2, 202, 208, 210, 211, 222, 228, 230, 232, 241, 243, 247, 251, 252, 255, 260, 262, 269, 270, 283, 289, 293, 303, 312, 523, 857]
surviving nonsolo ucounts = 40[48, 56, 69, 107, 123, 129, 130, 137, 152, 163, 167, 174, 181, 183, 187, 191, 200^2, 202, 208, 210, 211, 222, 228, 230, 232, 241, 243, 247, 251, 252, 255, 260, 262, 269, 270, 283, 289, 312, 523]
ids = [650, 285, 542, 724, 406, 114, 600, 360, 284, 604, ...]

====================================================================================

UMI info for barcode AGTCTTTCATCACGTA-1 contig 1 = AGGTTCCAGC...
umi CCATACGCTC = 57 reads: +19 -9 +382 -5 non-validated
umi CCCATCTGGC = 261 reads: +415 validated
umi CGGGGTTTTG = 312 reads: +415 validated
umi CTGAAAAGCT = 244 reads: +415 validated
umi GCCATCGCAG = 206 reads: +415 validated
umi TACACCGTCC = 199 reads: +415 validated
umi TAGCACTCCA = 280 reads: +415 validated
umi TCGCATCATT = 49 reads: +283 -30 +102 non-validated
umi TCGGACTCCG = 178 reads: +415 validated

UMI info for barcode AGTCTTTCATCACGTA-1 contig 2 = GGGGTCACAA...
umi AATCTGCTCT = 228 reads: +391 validated
umi AATTATCCAG = 202 reads: +391 validated
umi ACAGCATCAG = 191 reads: +391 validated
umi ACCCCGGGCG = 253 reads: +391 validated
umi ACCCCTTACG = 268 reads: +391 validated
umi ACCCTGCTTC = 225 reads: +391 validated
umi AGCTTCTTCC = 131 reads: +391 validated
umi AGGCTTTATT = 256 reads: +391 validated
umi ATAGAACCTT = 271 reads: +391 validated
umi CAAAACGGTT = 212 reads: +391 validated
umi CAAAGTAGCA = 224 reads: +391 validated
umi CAAGCCAGGC = 289 reads: +391 validated
umi CACAAACATA = 208 reads: +391 validated
umi CACGCAATTC = 182 reads: +391 validated
umi CACTTTCACG = 185 reads: +391 validated
umi CATCTTGGCG = 243 reads: +391 validated
umi CCATAAGTTA = 155 reads: +391 validated
umi CCTACATAGG = 185 reads: +391 validated
umi CCTCAGGGTT = 518 reads: +391 validated
umi CGGTGAACAC = 136 reads: +391 validated
umi CGTTTTTGGC = 197 reads: +391 validated
umi CTTCATGGGT = 211 reads: +391 validated
umi CTTTCGAGCA = 43 reads: -357X +1 -4XX +2 -4XX +2 -4XX +1 -9XX +1 -4XX +2 invalidated
umi GGTAAACCCG = 254 reads: +391 validated
umi GTAATTATCT = 68 reads: -6 +385 non-validated
umi GTTATATTAA = 792 reads: -351 +1 -2XX +1 -2XX +2 -1XX +5 -4XX +9 -1XX +1 -1XX +10 invalidated
umi TACATTTCGT = 266 reads: +391 validated
umi TAGTTAGTAG = 126 reads: +391 validated
umi TATATCGGCT = 162 reads: +391 validated
umi TATGAGCGAA = 248 reads: +391 validated
umi TGTTCAAACG = 108 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=544]
0-58 ==> 87-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
58-408 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=2)
404-424 ==> 0-20 on |10|IGHD1-26|D-REGION| [len=20] (mis=3)
425-473 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
473-544 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 8 umis using 156 reads
cdr3 = CARYSGSYRKTFDYW at 397, score = 9 + 7
umis assigned: [285, 291, 357, 400, 496, 577, 594, 650, 657]
of which 9 are surviving nonsolos
reads assigned: 1764
start codons at 14, 58, 102
confident = true

TIG 2[bases=640]
0-38 ==> 124-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
38-378 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=2)
391-429 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
429-640 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 29 umis using 835 reads
cdr3 = CCSYAGSSTFWVF at 362, score = 8 + 8
umis assigned: [33, 39, 45, 63, 65, 67, 114, 119, 136, 199] and 21 others
of which 29 are surviving nonsolos
reads assigned: 6907
start codons at 38, 177, 239, 246, 372
confident = true
now this is a cell
paired!

GCTGCGGACACGGCCGTGTATTACTGTGCGAGGTATAGTGGGAGCTACCGGAAGACCTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGGCTCCAGGCTGAGGACGAGGCTGATTATTACTGCTGCTCATATGCAGGTAGTAGCACTTTCTGGGTGTTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1159 = AGTCTTTCATCCGCGA-1

using 130 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 29
nonsolo ucounts = 20[2^3, 3, 4^2, 5^3, 6^3, 7^2, 8^2, 9^2, 11, 12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1167 = AGTCTTTCATTTGCCC-1

using 13 reads

====================================================================================

graph has 10 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1170 = AGTCTTTGTAAAGTCA-1

using 301 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 292]
surviving nonsolo ucounts = 1[292]
ids = [0]

====================================================================================

UMI info for barcode AGTCTTTGTAAAGTCA-1 contig 1 = GGGAATCAGT...
umi ACATGGCGGG = 281 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=500]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-500 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 276
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1174 = AGTCTTTGTAATCACC-1

using 285 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[3, 6, 272]
surviving nonsolo ucounts = 1[272]
ids = [3]

====================================================================================

UMI info for barcode AGTCTTTGTAATCACC-1 contig 1 = GGGGAGGAAC...
umi CTATCTTCAT = 273 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=560]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
386-424 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
424-560 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQYNNWPPRETF at 357, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 269
start codons at 36, 105, 241, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1182 = AGTCTTTGTATGGTTC-1

using 2196 reads

====================================================================================

graph has 3163 edges initially, 27 edges after simplification

total ucounts = 1050
nonsolo ucounts = 467[2^197, 3^117, 4^64, 5^32, 6^19, 7^15, 8^7, 9^3, 10^5, 11^2, 12^4, 14, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1196 = AGTCTTTGTGAGGGAG-1

using 51 reads

====================================================================================

graph has 30 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 3, 41]
surviving nonsolo ucounts = 1[41]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1199 = AGTCTTTGTGCAGGTA-1

using 1441 reads

====================================================================================

graph has 2168 edges initially, 28 edges after simplification

total ucounts = 714
nonsolo ucounts = 294[2^121, 3^73, 4^38, 5^25, 6^16, 7^9, 8^3, 9^3, 10, 11^2, 12, 13, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1223 = AGTCTTTTCAACGCTA-1

using 2526 reads

====================================================================================

graph has 2731 edges initially, 38 edges after simplification

total ucounts = 846
nonsolo ucounts = 389[2^150, 3^83, 4^50, 5^36, 6^26, 7^11, 8^12, 9^5, 10^2, 11^3, 12, 13^2, 16, 20^2, 26, 32, 162^2, 237]
surviving nonsolo ucounts = 1[237]
ids = [763]

====================================================================================

UMI info for barcode AGTCTTTTCAACGCTA-1 contig 1 = ACTCACTGGT...
umi TGTGCTCATT = 231 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=548]
0-114 ==> 61-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
114-477 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
478-517 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
517-548 ==> 0-31 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CQQYYSTPPYTF at 453, score = 9 + 8
umis assigned: [763]
of which 1 are surviving nonsolos
reads assigned: 229
start codons at 114, 183, 436
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1256 = AGTCTTTTCTACTTAC-1

using 31 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3^2, 4, 6, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1269 = AGTCTTTTCTTCGGTC-1

using 1792 reads

====================================================================================

graph has 2690 edges initially, 38 edges after simplification

total ucounts = 859
nonsolo ucounts = 388[2^163, 3^90, 4^62, 5^28, 6^16, 7^10, 8^11, 9^4, 10^2, 13, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1278 = AGTGAGGAGATACACA-1

using 310 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 8, 293]
surviving nonsolo ucounts = 1[293]
ids = [1]

====================================================================================

UMI info for barcode AGTGAGGAGATACACA-1 contig 1 = GAGTCAGACC...
umi AAACCTGCAT = 286 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=496]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=0)
377-416 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
416-496 ==> 0-80 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYNSYSPYTF at 352, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 279
start codons at 25, 31, 87, 100, 332, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1282 = AGTGAGGAGCCACGTC-1

using 361 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 354]
surviving nonsolo ucounts = 1[354]
ids = [4]

====================================================================================

UMI info for barcode AGTGAGGAGCCACGTC-1 contig 1 = GGAGGAACTG...
umi TTTGTAGATA = 353 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=555]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CQQYNNWPPWTF at 355, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 350
start codons at 34, 103, 239, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1291 = AGTGAGGAGCTGCGAA-1

using 392 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 8[2^3, 3, 4^3, 357]
surviving nonsolo ucounts = 1[357]
ids = [0]

====================================================================================

UMI info for barcode AGTGAGGAGCTGCGAA-1 contig 1 = GCTCTGGAGA...
umi AATATCCGGG = 327 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=501]
0-41 ==> 11-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
41-389 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=16)
388-426 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
426-501 ==> 0-75 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 50 reads
cdr3 = CQQYVSSPWTF at 365, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 323
start codons at 41, 249, 375, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1298 = AGTGAGGAGTACATGA-1

using 440 reads

====================================================================================

graph has 226 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2, 3^2, 5, 419]
surviving nonsolo ucounts = 1[419]
ids = [11]

====================================================================================

REJECT CONTIGS

TIG 1[bases=424]
0-171 ==> 166-337 on |357|IGLV3-19|L-REGION+V-REGION| [len=337] (mis=8)
175-213 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
213-424 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
cdr3 = CHSRDSRGPWVF at 149, score = 8 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 409
start codons at 33, 132
confident = false
not full
VJ delta = 12
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1300 = AGTGAGGAGTACGTTC-1

using 22 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[3^3, 7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1302 = AGTGAGGAGTCCTCCT-1

using 1516 reads

====================================================================================

graph has 1706 edges initially, 38 edges after simplification

total ucounts = 564
nonsolo ucounts = 298[2^115, 3^63, 4^34, 5^18, 6^19, 7^15, 8^5, 9^10, 10^6, 11^4, 14, 15^2, 16^3, 18, 19, 23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1303 = AGTGAGGAGTGGGCTA-1

using 275 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 272]
surviving nonsolo ucounts = 1[272]
ids = [0]

====================================================================================

UMI info for barcode AGTGAGGAGTGGGCTA-1 contig 1 = GAAATACTTT...
umi AAGCTTCGTT = 257 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=544]
0-40 ==> 61-101 on |195|IGHV4-61|5'UTR| [len=101] (mis=0)
40-396 ==> 0-356 on |196|IGHV4-61|L-REGION+V-REGION| [len=356] (mis=17)
407-455 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=6)
455-544 ==> 0-89 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CARDKYPGGFDYW at 385, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 251
start codons at 40, 84, 164, 355, 509
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1305 = AGTGAGGAGTGTGGCA-1

using 1595 reads

====================================================================================

graph has 1960 edges initially, 26 edges after simplification

total ucounts = 639
nonsolo ucounts = 309[2^113, 3^62, 4^44, 5^28, 6^23, 7^11, 8^6, 9^4, 10^8, 11^2, 12^2, 14, 15, 16^2, 18, 33]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1306 = AGTGAGGAGTTAGCGG-1

using 290 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[3^2, 4, 278]
surviving nonsolo ucounts = 1[278]
ids = [0]

====================================================================================

UMI info for barcode AGTGAGGAGTTAGCGG-1 contig 1 = GAAGAGCTGC...
umi ATAGAGTTTG = 281 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=557]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
384-421 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQYGSSPPLTF at 357, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 33, 241, 367, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1311 = AGTGAGGCAACTGGCC-1

using 230 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 225]
surviving nonsolo ucounts = 1[225]
ids = [1]

====================================================================================

UMI info for barcode AGTGAGGCAACTGGCC-1 contig 1 = GAGTGCTTTC...
umi AGCCTCACTG = 225 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=636]
18-389 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=40)
406-454 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=2)
454-636 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 28 reads
cdr3 = CARYFAFVNYYFDKW at 378, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 18, 39, 83
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1312 = AGTGAGGCAAGACGTG-1

using 17953 reads

====================================================================================

graph has 7399 edges initially, 60 edges after simplification

total ucounts = 842
nonsolo ucounts = 427[2^145, 3^76, 4^47, 5^33, 6^18, 7^14, 8^8, 9^5, 10^4, 11^3, 12^4, 13, 14^3, 15, 19, 29, 53, 73, 96, 109, 124, 135, 142^2, 153, 169, 174, 180, 184^2, 188, 197, 201, 205, 219, 221, 222, 226, 235, 238, 240, 246, 251^2, 255, 256, 258^2, 260, 261, 267, 272, 278, 280^2, 282, 286, 294, 295, 302, 303, 304, 312, 313, 315, 316, 319, 332, 333^2, 335, 341, 360, 368, 378, 391, 397, 400, 521]
surviving nonsolo ucounts = 61[96, 109, 124, 135, 142^2, 153, 169, 174, 180, 184^2, 188, 197, 201, 205, 219, 221, 222, 226, 235, 238, 240, 246, 251^2, 255, 256, 258^2, 260, 261, 267, 272, 278, 280^2, 282, 286, 294, 295, 302, 303, 304, 312, 313, 315, 316, 319, 332, 333^2, 335, 341, 360, 368, 378, 391, 397, 400, 521]
ids = [327, 75, 395, 486, 446, 662, 658, 791, 152, 840, ...]

====================================================================================

UMI info for barcode AGTGAGGCAAGACGTG-1 contig 1 = ATCACATAAC...
umi AATGTGCTCA = 288 reads: +442 -6 non-validated
umi AATTCAGTCG = 189 reads: +377 -1 +70 non-validated
umi AATTCTTGTC = 237 reads: +448 validated
umi AGTTACACAT = 264 reads: +448 validated
umi ATATATGGTG = 304 reads: +448 validated
umi ATATTTCCTA = 173 reads: +448 validated
umi ATTACCCCTC = 331 reads: +361 -1XX +86 invalidated
umi CCAGATTGGC = 277 reads: +444 -1 +3 non-validated
umi CCGACTTATA = 274 reads: +448 validated
umi CCGCATGCGT = 235 reads: +448 validated
umi CCTCAGCGCT = 98 reads: +331 -5 +1 -1 +19 -1 +3 -1X +71 -1 +13 -1 invalidated
umi CCTCTATCCG = 279 reads: +361 -1X +86 invalidated
umi CGTAATGCCC = 256 reads: +361 -1 +79 -2 +5 non-validated
umi CTAAAGCTCC = 122 reads: +341 -1 +2 -1 +3 -1 +1 -1 +6 -1 +3 -1X +86 invalidated
umi CTCATTTACC = 257 reads: +448 validated
umi CTGATAATGC = 10 reads: -366X +82 invalidated
umi CTGTCCACTC = 295 reads: +448 validated
umi CTGTGGTCTC = 261 reads: +430 -1X +17 invalidated
umi CTGTTTGTCC = 146 reads: +361 -1 +86 non-validated
umi GAGTTCTGTA = 135 reads: +357 -1X +90 invalidated
umi GTCCCTACAT = 282 reads: +436 -1 +11 non-validated
umi GTGGATCTCT = 202 reads: +350 -1X +6 -1 +75 -1 +14 invalidated
umi TACATTGAGC = 255 reads: +448 validated
umi TATATCTTCT = 200 reads: +448 validated
umi TATTTAGCAT = 194 reads: -17X +1 -3X +427 invalidated
umi TATTTCTCAC = 178 reads: +448 validated
umi TCAAGTTTAC = 211 reads: +433 -15 non-validated
umi TCCTCAACGC = 189 reads: +448 validated
umi TGACTCCTTT = 287 reads: +405 -1 +2 -1 +28 -11 non-validated
umi TGTTCTCGGG = 238 reads: +448 validated
umi TTCAAAGCCC = 239 reads: +448 validated
umi TTCATACCCA = 170 reads: +448 validated
umi TTTCCGCATG = 221 reads: +361 -1 +71 -1 +1 -13 non-validated
umi TTTTCCCACG = 178 reads: +432 -1 +2 -2 +2 -1 +1 -7 non-validated

UMI info for barcode AGTGAGGCAAGACGTG-1 contig 2 = TGGGGGATCA...
umi AACTTGCATA = 331 reads: +397 validated
umi AAGAACAACC = 313 reads: +397 validated
umi AATCACCTTT = 320 reads: +397 validated
umi ACACATTTAT = 253 reads: +397 validated
umi ACCTTTCAAC = 108 reads: +397 validated
umi ACTTGGGATG = 371 reads: +397 validated
umi AGAGAGACCG = 391 reads: +397 validated
umi ATCAAACGTA = 330 reads: +397 validated
umi CATTTAGTTC = 359 reads: +397 validated
umi CCATTGACAT = 268 reads: +397 validated
umi CCCGGTAATG = 339 reads: +397 validated
umi CCCTAGTTGA = 302 reads: +397 validated
umi CCTGATCTGA = 383 reads: +397 validated
umi CGCTTGTCGT = 319 reads: +397 validated
umi CTGCTAGACA = 258 reads: +397 validated
umi GCATCTCTGG = 530 reads: +58 -1XX +4 -1XX +3 -1XX +1 -1XX +1 -3XX +1 -1XX +1 -1XX +1 -211XX +1 -1XX +2 -8XX +1 -3XX +1 -7XX +83 invalidated
umi GCTCCCGTGT = 332 reads: +397 validated
umi GTGTCAAGAC = 399 reads: +397 validated
umi TAAACTCTAG = 283 reads: +397 validated
umi TAGGCCGTTC = 150 reads: +397 validated
umi TATACCGTTA = 144 reads: +397 validated
umi TATATCATCA = 304 reads: +397 validated
umi TCTCGTATGA = 229 reads: +397 validated
umi TCTTATTAGC = 315 reads: +397 validated
umi TGCTTATCGT = 399 reads: +397 validated
umi TTACGAATCC = 316 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=577]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=11)
443-506 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=6)
506-577 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 21 umis using 248 reads
cdr3 = CARGGWATGTTDLEKGYYYYAMDVW at 400, score = 9 + 7
umis assigned: [38, 40, 41, 138, 150, 152, 181, 282, 312, 315] and 24 others
of which 34 are surviving nonsolos
reads assigned: 7351
start codons at 58, 209, 256, 355, 463
confident = true

TIG 2[bases=568]
35-395 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=6)
394-432 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 25 umis using 1078 reads
cdr3 = CMQALQTRETF at 371, score = 9 + 8
umis assigned: [24, 25, 31, 48, 75, 95, 103, 153, 272, 289] and 16 others
of which 26 are surviving nonsolos
reads assigned: 7903
start codons at 35, 68, 104, 192, 354, 374, 474
confident = true
now this is a cell
paired!

AGAGGGGGGTGGGCAACTGGAACAACCGATCTGGAGAAGGGCTACTACTATTACGCTATGGACGTCTGGGGCCAAGGGACCGCGGTCACCGTCTCCTCAG <==> ATCAGCAGAGTGGAGGCTGAGGATGTTGGGGTTTATTACTGCATGCAAGCTCTACAAACTCGGGAGACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1316 = AGTGAGGCAAGGCTCC-1

using 275 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[274]
surviving nonsolo ucounts = 1[274]
ids = [1]

====================================================================================

UMI info for barcode AGTGAGGCAAGGCTCC-1 contig 1 = GTCAGACTCA...
umi TCACAACGAT = 271 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=17)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQYNSYALSF at 350, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 23, 29, 85, 98, 234, 237, 330, 369, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1322 = AGTGAGGCACACTGCG-1

using 10430 reads

====================================================================================

graph has 4868 edges initially, 66 edges after simplification

total ucounts = 912
nonsolo ucounts = 410[2^163, 3^73, 4^37, 5^37, 6^17, 7^18, 8^11, 9^7, 10^6, 11^2, 12^4, 13^2, 14^2, 15, 17, 20, 21, 140, 142, 146, 165, 178, 225, 227, 228, 248, 253, 254, 259, 261, 264, 266, 289^2, 297, 309, 338, 375, 379, 420, 488, 584, 607, 783]
surviving nonsolo ucounts = 26[140, 142, 146, 165, 178, 225, 227, 228, 248, 253, 254, 259, 261, 264, 266, 289^2, 297, 309, 338, 375, 379, 420, 488, 584, 607]
ids = [42, 674, 293, 710, 551, 445, 407, 444, 395, 137, ...]

====================================================================================

UMI info for barcode AGTGAGGCACACTGCG-1 contig 1 = GCTCTGCTTC...
umi AAGGGGATAT = 269 reads: +391 validated
umi AGCCCACAGA = 257 reads: +391 validated
umi CATCACGTGC = 148 reads: +391 validated
umi CCATGGACTC = 810 reads: -357X +1 -2X +5 -4XX +9 -1XX +1 -1XX +10 invalidated
umi CCCCTTCACA = 255 reads: +391 validated
umi CGGAACACTA = 250 reads: +391 validated
umi CGTCTTCCGG = 229 reads: +391 validated
umi CTATAGCTTG = 343 reads: +391 validated
umi CTGCCATCAG = 31 reads: -362X +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi CTGCGCCTTA = 226 reads: +391 validated
umi GACATGACCT = 288 reads: +391 validated
umi GACGGCACTT = 262 reads: +391 validated
umi GCGTTGTCGC = 181 reads: +391 validated
umi GCTGCGATCA = 39 reads: -356X +2 -4X +2 -3XX +1 -1XX +1 -4XX +1 -3XX +1 -1XX +1 -3XX +1 -4XX +2 invalidated
umi GGCTAATTAC = 292 reads: +391 validated
umi GTACAGACTA = 313 reads: +391 validated
umi TATGAGCTCA = 167 reads: +391 validated
umi TGTTTGACGG = 587 reads: +391 validated
umi TTATCTTGAG = 258 reads: +391 validated
umi TTGCTGAGAA = 263 reads: +391 validated

UMI info for barcode AGTGAGGCACACTGCG-1 contig 2 = AGCATCATCC...
umi TAACTCTAAA = 1 reads: -369X +1 -1 +1 -1 +1 -1 +2 -4X +5 -1 +2 -1 +1 -2 +2 -1 +1 -1 +1 -4X +1 -1 +2 -1 +1 -3X +7 -1X +1 -1 +1 -7 invalidated
umi TAACTCTTTT = 140 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=653]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=0)
404-442 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
442-653 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 17 umis using 779 reads
cdr3 = CQSYDSSLSGSVF at 375, score = 8 + 8
umis assigned: [49, 137, 293, 319, 331, 395, 407, 422, 444, 445] and 10 others
of which 19 are surviving nonsolos
reads assigned: 5339
start codons at 51, 205, 208, 259, 358, 385, 574
confident = true

TIG 2[bases=517]
0-61 ==> 243-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=1)
61-414 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=20)
443-491 ==> 13-61 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
491-517 ==> 0-26 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CARGDRRAAAFYYHYMDVW at 403, score = 8 + 7
umis assigned: [673, 674]
of which 1 are surviving nonsolos
reads assigned: 138
start codons at 61, 217, 259, 325, 358, 448
confident = true

REJECT CONTIGS

TIG 1[bases=490]
0-60 ==> 19-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
0-61 ==> 5939-6000 on rc of segment after IGHV3-41 exon 1 [len=6000] (mis=5)
24-71 ==> 3154-3201 on rc of segment before IGHV3-33 exon 2 [len=3201] (mis=6)
53-76 ==> 6531-6554 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=0)
60-413 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=3)
417-444 ==> 0-27 on |22|IGHD3-9|D-REGION| [len=27] (mis=0)
446-490 ==> 0-44 on |53|IGHJ3|J-REGION| [len=50] (mis=3)
cdr3 = CAKMGVLRYFDWLLEGAFDIW at 402, score = 9 + 8
umis assigned: [42, 540, 579]
of which 3 are surviving nonsolos
reads assigned: 745
start codons at 60, 211, 216, 363, 411, 477
confident = false
VJ delta = 10
not full
not full

TIG 2[bases=306]
3-186 ==> 169-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=4)
184-235 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
235-306 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [173, 566, 768]
of which 3 are surviving nonsolos
reads assigned: 1420
start codons at 5, 77, 179
confident = false
did not find CDR3
now this is a cell
paired!

GCCGTGTATCATTGTGCTAGAGGGGATCGTAGAGCAGCCGCTTTCTATTACCACTACATGGACGTCTGGGGCAAAGGGACCACAGTCACCGTCTCCTCAG <==> GGGCTCCAGGCTGAGGATGAGGCTGATTATTACTGCCAGTCCTATGACAGCAGCCTGAGTGGTTCAGTCTTCGGAACTGGGACCAAGGTCACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1328 = AGTGAGGCACCCTATC-1

using 204 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 24, 175]
surviving nonsolo ucounts = 1[175]
ids = [1]

====================================================================================

UMI info for barcode AGTGAGGCACCCTATC-1 contig 1 = GAGAGAGTAG...
umi CTTTTCACTT = 170 reads: +424 validated

GOOD CONTIGS

TIG 1[bases=518]
0-73 ==> 6-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=1)
73-426 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
450-497 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
497-518 ==> 0-21 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CARDRAATARLGGMDVW at 415, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 169
start codons at 73, 224, 229, 376, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1330 = AGTGAGGCACCTATCC-1

using 15 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1339 = AGTGAGGCAGCTCCGA-1

using 52 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[52]
surviving nonsolo ucounts = 1[52]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=359]
0-51 ==> 86-137 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=0)
54-257 ==> 137-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=9)
261-299 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=6)
299-359 ==> 0-60 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CCSYAGSSAF at 241, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 36
start codons at 53, 56, 125, 251
confident = false
see insertion of TGA at pos 137 on |343|IGLV2-23|L-REGION+V-REGION|
not full
VJ delta = 3
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1343 = AGTGAGGCATATACCG-1

using 55 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 4[2, 4^2, 35]
surviving nonsolo ucounts = 1[35]
ids = [10]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1346 = AGTGAGGCATCTCCCA-1

using 1424 reads

====================================================================================

graph has 529 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2, 273, 327, 816]
surviving nonsolo ucounts = 2[327, 816]
ids = [2, 3]

====================================================================================

UMI info for barcode AGTGAGGCATCTCCCA-1 contig 1 = AGGAGTCAGT...
umi ACTGTGCTAG = 333 reads: +385 validated
umi AGGTCACACT = 819 reads: +385 validated
umi GGCTAATCCC = 188 reads: +2 -1XX +38 -1XX +10 -1XX +14 -1XX +2 -1XX +2 -1XX +21 -2XX +1 -1XX +9 -3XX +19 -21X +7 -2XX +1 -2XX +1 -4XX +11 -1XX +5 -1XX +68 -1XX +39 -1XX +1 -1XX +2 -1XX +2 -2XX +19 -1XX +3 -20X +2 -3XX +1 -1XX +1 -3XX +4 -1XX +9 -1XX +4 -1XX +7 invalidated

GOOD CONTIGS

TIG 1[bases=548]
0-27 ==> 20-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
27-370 ==> 0-343 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=13)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 168 reads
cdr3 = CQQSYNMWTF at 354, score = 9 + 8
umis assigned: [2, 3, 7]
of which 2 are surviving nonsolos
reads assigned: 1307
start codons at 27, 33, 89, 102, 238, 372, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1351 = AGTGAGGCATTATCTC-1

using 942 reads

====================================================================================

graph has 338 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[3^2, 248, 290, 390]
surviving nonsolo ucounts = 3[248, 290, 390]
ids = [12, 5, 2]

====================================================================================

UMI info for barcode AGTGAGGCATTATCTC-1 contig 1 = AGCATCATCC...
umi TTTCGCTGGG = 245 reads: +442 validated

UMI info for barcode AGTGAGGCATTATCTC-1 contig 2 = GCTCTGCTTC...
umi CGTGCCGTTA = 277 reads: +391 validated

UMI info for barcode AGTGAGGCATTATCTC-1 contig 3 = AGTCTCAGTC...
umi ACGCCACATC = 365 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=574]
0-61 ==> 243-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=0)
61-414 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=2)
420-447 ==> 0-27 on |22|IGHD3-9|D-REGION| [len=27] (mis=2)
455-503 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
503-574 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CARNPLYYDILTGYSHVGYFDYW at 403, score = 9 + 7
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 61, 217, 259, 325, 358
confident = false

TIG 2[bases=548]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=22)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=6)
442-548 ==> 0-106 on |307|IGLC3|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 46 reads
cdr3 = CQSYDSILTGWAF at 375, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 51, 205, 358, 385, 521
confident = false

TIG 3[bases=489]
0-20 ==> 3-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
20-373 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=3)
369-408 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
408-489 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CQQSYSTPYTF at 347, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 358
start codons at 20, 26, 82, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1356 = AGTGAGGGTAGAAAGG-1

using 159 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 19
nonsolo ucounts = 8[2^7, 134]
surviving nonsolo ucounts = 1[134]
ids = [16]

====================================================================================

UMI info for barcode AGTGAGGGTAGAAAGG-1 contig 1 = GGAATCAGTC...
umi TGTGTCGTGG = 117 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=502]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-502 ==> 0-88 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [16]
of which 1 are surviving nonsolos
reads assigned: 116
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

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BARCODE 12.1357 = AGTGAGGGTCAAAGAT-1

using 320 reads

====================================================================================

graph has 142 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[320]
surviving nonsolo ucounts = 1[320]
ids = [0]

====================================================================================

UMI info for barcode AGTGAGGGTCAAAGAT-1 contig 1 = TGTGATCAGT...
umi ACGTTTTCGT = 322 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=3)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
377-415 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CLQHKSYPFTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 316
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1372 = AGTGAGGGTGTCTGAT-1

using 119 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 112]
surviving nonsolo ucounts = 1[112]
ids = [4]

====================================================================================

UMI info for barcode AGTGAGGGTGTCTGAT-1 contig 1 = GAGCATCACC...
umi TCTTCCCCTC = 92 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=501]
0-62 ==> 2-64 on |66|IGHV1-2|5'UTR| [len=64] (mis=0)
62-415 ==> 0-353 on |67|IGHV1-2|L-REGION+V-REGION| [len=353] (mis=23)
426-474 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
474-501 ==> 0-27 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CARGGGNSWFDYW at 404, score = 7 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 92
start codons at 62, 218, 260, 265, 297, 326, 359
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1378 = AGTGAGGGTTCATGGT-1

using 248 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 21
nonsolo ucounts = 12[2^5, 3, 4^2, 5^2, 6, 202]
surviving nonsolo ucounts = 1[202]
ids = [12]

====================================================================================

UMI info for barcode AGTGAGGGTTCATGGT-1 contig 1 = GGCTGGGGTC...
umi CTTTCTGCAG = 199 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=561]
42-403 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
392-430 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
430-561 ==> 0-131 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CSSYTSSSTLVF at 366, score = 8 + 9
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 197
start codons at 42, 199, 243, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 12.1380 = AGTGAGGGTTCGCGAC-1

using 356 reads

====================================================================================

graph has 361 edges initially, 4 edges after simplification

total ucounts = 225
nonsolo ucounts = 53[2^32, 3^7, 4^6, 5^2, 6^2, 7, 13, 16, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.11
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk012-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk012-ucc6a7a72ce/files/contig_bam.bam

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78.401 seconds used processing barcodes, peak mem = 0.30
