[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.36 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk009-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk009-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk009.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.18 = AGATCTGAGAGTGACC-1

using 105 reads

====================================================================================

graph has 157 edges initially, 2 edges after simplification

total ucounts = 22
nonsolo ucounts = 16[2^4, 3^2, 4^2, 6^3, 9, 10, 12, 14^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.22 = AGATCTGAGATGCCTT-1

using 11321 reads

====================================================================================

graph has 3954 edges initially, 44 edges after simplification

total ucounts = 354
nonsolo ucounts = 148[2^38, 3^23, 4^15, 5^8, 6^4, 7, 8^2, 9^2, 10^3, 11, 12, 21, 35, 48, 64, 84, 98, 101, 104, 105, 108, 118, 121, 139, 148, 149, 161, 166, 167, 177^3, 187^2, 195, 201, 202, 204, 210, 224, 234, 235, 242, 253, 259, 265, 266, 267, 272, 273, 274, 279, 313, 319, 330, 344, 347, 391, 406, 544, 561]
surviving nonsolo ucounts = 47[48, 84, 98, 101, 104, 105, 108, 118, 121, 139, 148, 149, 161, 166, 167, 177^3, 187^2, 195, 201, 202, 204, 210, 224, 234, 235, 242, 253, 259, 265, 266, 267, 272, 273, 274, 279, 313, 319, 330, 344, 347, 391, 406, 544, 561]
ids = [122, 89, 212, 69, 118, 247, 183, 19, 12, 97, ...]

====================================================================================

UMI info for barcode AGATCTGAGATGCCTT-1 contig 1 = AGGAGTCAGA...
umi AACAGTCCGA = 266 reads: +388 validated
umi AATATCACGG = 311 reads: +388 validated
umi ACACAACGGT = 204 reads: +388 validated
umi ACTGCGTTAC = 334 reads: +56 -1XX +1 -1XX +2 -1XX +1 -5XX +4 -20XX +1 -3XX +3 -11XX +278 invalidated
umi AGACGCGTTT = 278 reads: -320X +1 -1XX +1 -2XX +1 -3XX +1 -1XX +1 -5XX +1 -10XX +1 -1XX +30 -1XX +7 invalidated
umi AGCACTGGCT = 314 reads: +388 validated
umi ATATTTACAG = 259 reads: +388 validated
umi ATCGTATTTG = 405 reads: +388 validated
umi ATGGCACGGC = 83 reads: +2 -1 +2 -1 +382 non-validated
umi CACGGTAGTA = 278 reads: +388 validated
umi CCTGTATTGG = 250 reads: +140 -1XX +247 invalidated
umi CTACATATTA = 227 reads: +388 validated
umi CTACGCACAA = 264 reads: +388 validated
umi CTTACGTGCT = 348 reads: +388 validated
umi CTTATGTTGC = 109 reads: +388 validated
umi GCTTACCCCA = 271 reads: +388 validated
umi GGGCCTCTGC = 563 reads: +388 validated
umi GTCCCGGGAA = 271 reads: +388 validated
umi TAAACACTAA = 263 reads: +388 validated
umi TCATCAATGT = 194 reads: +344 -1XX +1 -2XX +40 invalidated
umi TGAGTTCAAG = 342 reads: +388 validated
umi TTCAGTCATG = 277 reads: +388 validated

UMI info for barcode AGATCTGAGATGCCTT-1 contig 2 = TGGGGAGGTC...
umi AATGTACATC = 180 reads: +439 validated
umi AATTTCCGGC = 173 reads: +439 validated
umi AGCACTTGAC = 263 reads: +36 -1XX +402 invalidated
umi CAAAAATCTT = 181 reads: +382 -1 +1 -4X +1 -1 +2 -1 +5 -3X +1 -5X +28 -4 invalidated
umi CAAACCGCCG = 141 reads: +439 validated
umi CAAATTGCTA = 148 reads: +431 -1 +1 -1 +4 -1 non-validated
umi CATACGACAG = 236 reads: +439 validated
umi CCATGCATTA = 104 reads: +375 -8 +56 non-validated
umi CCATGCCACT = 170 reads: +439 validated
umi CCATTGTCCG = 3 reads: -399X +2 -1XX +1 -1XX +35 invalidated
umi CCGATACCTT = 298 reads: -110X +329 invalidated
umi CCGTATCCGT = 228 reads: +437 -2 non-validated
umi CGAAGGCCTA = 206 reads: +439 validated
umi GACTCTCTTA = 201 reads: +383 -1 +3 -52 non-validated
umi GATATCGCGT = 193 reads: +430 -9 non-validated
umi GCCACGCACG = 99 reads: +435 -2 +2 non-validated
umi GCTGTTAACG = 159 reads: +385 -1 +22 -31 non-validated
umi GTATAAAGCT = 103 reads: +369 -1 +3 -7 +1 -1 +1 -1 +4 -1 +1 -1 +1 -1 +1 -1 +8 -3 +1 -2 +1 -1 +3 -1 +5 -1 +1 -1 +3 -13 non-validated
umi TACTGGGCCA = 146 reads: +427 -3 +7 -1 +1 non-validated
umi TAGGGTTCCA = 212 reads: +439 validated
umi TATTCATGCC = 168 reads: +376 -1 +3 -1 +1 -1 +1 -1 +1 -1 +52 non-validated
umi TTTGATTCCC = 181 reads: +439 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=12)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 21 umis using 883 reads
cdr3 = CQQYNNYSWTF at 354, score = 8 + 8
umis assigned: [5, 17, 26, 47, 52, 56, 72, 79, 89, 103] and 12 others
of which 22 are surviving nonsolos
reads assigned: 6002
start codons at 27, 33, 89, 102, 238, 241, 334, 457
confident = true

TIG 2[bases=636]
84-437 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=21)
477-523 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
523-636 ==> 0-113 on |3|IGHA2|C-REGION| [len=1019] (mis=0)
junction support: 12 umis using 237 reads
cdr3 = CASMILGSDLLRMGGTPFFELW at 426, score = 8 + 7
umis assigned: [22, 24, 57, 96, 97, 99, 110, 118, 119, 122] and 12 others
of which 22 are surviving nonsolos
reads assigned: 3711
start codons at 84, 235, 240, 298, 301, 387, 435, 462, 577, 596
confident = true
now this is a cell
paired!

CACTGTGCGAGTATGATCCTGGGTTCGGACCTCTTAAGAATGGGGGGGACTCCGTTCTTTGAACTCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGATGATTTTGCAACTTATTACTGCCAACAGTATAATAATTATTCGTGGACGTTCGGCCAAGGGACCAAGGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.24 = AGATCTGAGCCAACAG-1

using 361 reads

====================================================================================

graph has 136 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[3, 353]
surviving nonsolo ucounts = 1[353]
ids = [5]

====================================================================================

UMI info for barcode AGATCTGAGCCAACAG-1 contig 1 = AGAGCTCTGG...
umi CCGTTTGTTG = 337 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=510]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
392-429 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
429-510 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 56 reads
cdr3 = CQQYGSSPPTF at 368, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 331
start codons at 44, 252, 378, 471
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.33 = AGATCTGAGGCGATAC-1

using 554 reads

====================================================================================

graph has 842 edges initially, 6 edges after simplification

total ucounts = 281
nonsolo ucounts = 102[2^50, 3^20, 4^15, 5^5, 6^2, 7^2, 10^4, 11, 12, 15, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.39 = AGATCTGAGTCCGTAT-1

using 48 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2, 13, 32]
surviving nonsolo ucounts = 1[32]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.43 = AGATCTGAGTGGTAAT-1

using 443 reads

====================================================================================

graph has 152 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 185, 251]
surviving nonsolo ucounts = 2[185, 251]
ids = [4, 1]

====================================================================================

UMI info for barcode AGATCTGAGTGGTAAT-1 contig 1 = GCTGGGGTCT...
umi AGCTCAGTTC = 244 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=578]
0-41 ==> 122-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
41-395 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
391-429 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
429-578 ==> 0-149 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CSSHAGSTRVLF at 365, score = 8 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 239
start codons at 41, 198, 249, 348, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.51 = AGATCTGCAATGTAAG-1

using 46 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[44]
surviving nonsolo ucounts = 1[44]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.66 = AGATCTGCAGTCAGAG-1

using 1088 reads

====================================================================================

graph has 1614 edges initially, 10 edges after simplification

total ucounts = 492
nonsolo ucounts = 233[2^88, 3^59, 4^43, 5^16, 6^10, 7^3, 8^3, 9^6, 10, 12^2, 15^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.78 = AGATCTGGTAGCGTGA-1

using 152 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[4, 146]
surviving nonsolo ucounts = 1[146]
ids = [2]

====================================================================================

UMI info for barcode AGATCTGGTAGCGTGA-1 contig 1 = AGCATCGGGG...
umi TCCTTGCACG = 141 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=479]
0-21 ==> 94-115 on |368|IGLV3-9|5'UTR| [len=115] (mis=0)
21-361 ==> 0-340 on |369|IGLV3-9|L-REGION+V-REGION| [len=346] (mis=6)
359-397 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
397-479 ==> 0-82 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CQAWDSSTVIF at 336, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 140
start codons at 21, 82, 169, 319
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.79 = AGATCTGGTAGCTTGT-1

using 236 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[233]
surviving nonsolo ucounts = 1[233]
ids = [0]

====================================================================================

UMI info for barcode AGATCTGGTAGCTTGT-1 contig 1 = GAGTGCTTTC...
umi AATCCCGGTG = 231 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=549]
18-389 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=8)
406-454 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
454-549 ==> 0-95 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CARAHGDYYTLLDFW at 378, score = 8 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 18, 39, 83, 169, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.88 = AGATCTGGTCGAACAG-1

using 256 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[254]
surviving nonsolo ucounts = 1[254]
ids = [0]

====================================================================================

UMI info for barcode AGATCTGGTCGAACAG-1 contig 1 = ATCACATAAC...
umi ATGCTTCCTC = 258 reads: +448 validated

GOOD CONTIGS

TIG 1[bases=608]
0-58 ==> 3-61 on |84|IGHV1-69|5'UTR| [len=61] (mis=0)
58-411 ==> 0-353 on |85|IGHV1-69|L-REGION+V-REGION| [len=353] (mis=0)
420-441 ==> 0-21 on |32|IGHD6-13|D-REGION| [len=21] (mis=0)
443-506 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=2)
506-608 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CARDHPPGIAAAGTLYYYYYGMDVW at 400, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 250
start codons at 58, 209, 256, 355, 463, 524, 585
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.92 = AGATCTGGTCTCCACT-1

using 343 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[9, 328]
surviving nonsolo ucounts = 1[328]
ids = [5]

====================================================================================

UMI info for barcode AGATCTGGTCTCCACT-1 contig 1 = GGGAATCAGT...
umi TATTACAACA = 328 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=1)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 55 reads
cdr3 = CLQYSSSPWTF at 354, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 325
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.94 = AGATCTGGTCTTGTCC-1

using 3575 reads

====================================================================================

graph has 1723 edges initially, 26 edges after simplification

total ucounts = 228
nonsolo ucounts = 98[2^27, 3^15, 4^7, 5^10, 6^8, 7^3, 8^4, 9^5, 10, 12^5, 13, 15, 18, 30, 91, 109, 176, 182, 248, 259, 318, 614, 979]
surviving nonsolo ucounts = 10[30, 91, 109, 176, 182, 248, 259, 318, 614, 979]
ids = [11, 85, 180, 33, 36, 88, 204, 68, 226, 79]

====================================================================================

UMI info for barcode AGATCTGGTCTTGTCC-1 contig 1 = TGGGGAGGAA...
umi ACACCATCGG = 30 reads: -382 non-validated
umi AGGATTGCGT = 179 reads: +382 validated
umi CGCACGAGGG = 310 reads: -318 +1 -3X +60 invalidated
umi CGTACGAGTC = 986 reads: +382 validated
umi GAAACACCTC = 249 reads: +382 validated
umi TGGCATACCA = 262 reads: +382 validated
umi TTTGTGCATG = 621 reads: -98 +284 non-validated

GOOD CONTIGS

TIG 1[bases=555]
0-37 ==> 10-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
37-382 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 443 reads
cdr3 = CQQRSNWPPTF at 358, score = 9 + 8
umis assigned: [11, 33, 68, 79, 88, 204, 226]
of which 7 are surviving nonsolos
reads assigned: 2589
start codons at 37, 242, 245, 461
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.98 = AGATCTGGTGCTGTAT-1

using 271 reads

====================================================================================

graph has 78 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[271]
surviving nonsolo ucounts = 1[271]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=467]
0-28 ==> 24-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-29 ==> 5708-5737 on segment before IGKV3OR2-5 exon 1 [len=6000] (mis=1)
28-236 ==> 0-208 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
236-356 ==> 228-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3) [SHIFT!]
381-467 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQHYGSSPRTLLAKIKRTVAAPSVFIFPPSDEQLKS at 332, score = 9 + 4
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 250
start codons at 28, 77, 342, 423
confident = false
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.106 = AGATCTGTCACAACGT-1

using 320 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[3, 4, 6, 11, 14, 276]
surviving nonsolo ucounts = 1[276]
ids = [5]

====================================================================================

UMI info for barcode AGATCTGTCACAACGT-1 contig 1 = AGCTCAGCTT...
umi GGATAGGTTC = 271 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-52 ==> 62-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
52-360 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
402-440 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
440-551 ==> 0-111 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CVAWDDSLYAWVF at 373, score = 8 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 269
start codons at 52, 356, 386, 398
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.107 = AGATCTGTCACGCATA-1

using 344 reads

====================================================================================

graph has 144 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2^2, 5, 10, 13, 309]
surviving nonsolo ucounts = 1[309]
ids = [3]

====================================================================================

UMI info for barcode AGATCTGTCACGCATA-1 contig 1 = GGAGTCAGAC...
umi CGGCAGGTCT = 309 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=8)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQYNSYPLTF at 353, score = 8 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 304
start codons at 26, 32, 88, 101, 237, 240, 333, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.114 = AGATCTGTCCAAGTAC-1

using 19 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.115 = AGATCTGTCCACGACG-1

using 285 reads

====================================================================================

graph has 146 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 7, 9, 258]
surviving nonsolo ucounts = 1[258]
ids = [2]

====================================================================================

UMI info for barcode AGATCTGTCCACGACG-1 contig 1 = AGTCAGACTC...
umi ATCATATGGC = 236 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=498]
0-24 ==> 157-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
24-375 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=21)
373-412 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
412-498 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQQYYTFSHTF at 351, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 236
start codons at 24, 30, 86, 99, 182, 235, 238, 331, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.116 = AGATCTGTCCACGCAG-1

using 35 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[4, 5, 6, 7, 8]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.118 = AGATCTGTCCAGATCA-1

using 47193 reads

====================================================================================

graph has 15118 edges initially, 164 edges after simplification

total ucounts = 1965
nonsolo ucounts = 1167[2^290, 3^186, 4^110, 5^94, 6^70, 7^43, 8^45, 9^38, 10^25, 11^26, 12^23, 13^12, 14^16, 15^21, 16^12, 17^9, 18^3, 19^2, 20, 21, 22, 23^2, 26, 29, 32, 55, 56, 58, 76, 77, 79, 80, 83, 92, 93^2, 97, 105, 110, 122, 131, 133, 137, 152, 153, 161, 173, 176, 177, 179, 186, 203, 211, 221, 222, 226, 227, 228^2, 243, 248, 254, 255, 257, 262, 263, 265, 266, 269^2, 271, 273, 275, 278^2, 279, 283, 284, 290, 292, 293, 295, 296, 297, 299^2, 302, 303^2, 305^2, 306, 307, 308, 311, 313, 315, 317, 319, 321, 322^2, 323, 326^2, 327, 328^2, 329, 331^2, 332, 336^2, 343, 345, 346, 349, 350, 352, 353, 355, 359^2, 360, 364, 367, 369^2, 371, 375^2, 376^4, 382, 384, 386, 389^2, 399, 400, 403, 405, 416, 425^2, 428, 440, 458, 461, 557, 565, 614, 667, 718, 719, 1223]
surviving nonsolo ucounts = 129[2^2, 5, 56, 58, 79, 97, 105, 110, 122, 131, 133, 137, 152, 153, 161, 173, 176, 177, 179, 186, 203, 211, 221, 222, 226, 227, 228^2, 243, 248, 254, 255, 257, 262, 263, 265, 266, 269^2, 271, 273, 275, 278^2, 279, 283, 284, 290, 292, 293, 295, 296, 297, 299^2, 302, 303^2, 305^2, 306, 307, 308, 311, 313, 315, 317, 319, 321, 322^2, 323, 326^2, 327, 328^2, 329, 331^2, 332, 336^2, 343, 345, 346, 349, 350, 352, 353, 355, 359^2, 360, 364, 367, 369^2, 371, 375^2, 376^4, 382, 384, 386, 389^2, 399, 400, 403, 405, 416, 425^2, 428, 440, 458, 461, 557, 565, 614, 667, 718, 719, 1223]
ids = [505, 1069, 1299, 1936, 447, 1007, 575, 864, 14, 1947, ...]

====================================================================================

UMI info for barcode AGATCTGTCCAGATCA-1 contig 1 = GGGAGAGCCC...
umi AAAGCAGGAT = 110 reads: +382 validated
umi AAATCTTTCA = 366 reads: +382 validated
umi AACACCCGTC = 329 reads: +382 validated
umi AACCAGCTGT = 428 reads: +382 validated
umi AAGCCACTCG = 381 reads: +382 validated
umi AAGGTCCACA = 713 reads: +382 validated
umi AAGTGGTTTC = 311 reads: +382 validated
umi AATAAATGCA = 388 reads: +382 validated
umi AATAGGAGTG = 323 reads: +382 validated
umi AATCTAATAG = 301 reads: +382 validated
umi AATGACTGGG = 300 reads: +382 validated
umi ACAATGCTTA = 352 reads: +382 validated
umi ACACATAGTA = 326 reads: +382 validated
umi ACCATAAGGG = 154 reads: +338 -33 +11 non-validated
umi ACCCCCTCAA = 354 reads: +382 validated
umi ACGCCCCTAT = 181 reads: +382 validated
umi ACGCTACGCT = 361 reads: +382 validated
umi ACGCTTGGGT = 308 reads: +382 validated
umi ACGGCTGTCA = 373 reads: +382 validated
umi ACGGTATACC = 322 reads: +382 validated
umi ACTAGGCCCT = 322 reads: +382 validated
umi ACTATGCCAG = 396 reads: +382 validated
umi ACTCAGCAGC = 202 reads: +382 validated
umi AGCAGATCCA = 228 reads: +382 validated
umi AGCCCTCTTC = 305 reads: +382 validated
umi AGTCCATCTA = 266 reads: +382 validated
umi ATACCTTGAT = 460 reads: +382 validated
umi ATTCAGCTCG = 280 reads: +382 validated
umi ATTCCAAGCT = 351 reads: +382 validated
umi ATTCTCGCCT = 484 reads: +258 -3XX +121 invalidated
umi ATTTACGATG = 303 reads: +382 validated
umi CACACGATAT = 406 reads: +382 validated
umi CACTTTACAC = 276 reads: +382 validated
umi CAGCCGGCTT = 364 reads: +382 validated
umi CAGGTCCTTC = 616 reads: -247 +1 -1XX +133 invalidated
umi CAGTATATGG = 383 reads: +382 validated
umi CAGTCGGGCT = 261 reads: +382 validated
umi CATAGAATAT = 311 reads: +382 validated
umi CATATTGAGC = 98 reads: +382 validated
umi CATCCTAAGG = 302 reads: +382 validated
umi CCACGTCAGC = 346 reads: +382 validated
umi CCAGCAACGA = 259 reads: +382 validated
umi CCGTTTTCAG = 302 reads: +382 validated
umi CCTCCTTTGC = 279 reads: +382 validated
umi CCTGCGAACG = 315 reads: +382 validated
umi CCTTCCTATA = 350 reads: +382 validated
umi CGACATGAAT = 328 reads: +382 validated
umi CGAGCCTTTT = 327 reads: +382 validated
umi CGCATCACAT = 262 reads: +382 validated
umi CGCCGTACAG = 372 reads: +382 validated
umi CGGAGAGGCT = 222 reads: +382 validated
umi CTAAGCAATT = 443 reads: +382 validated
umi CTACGTCGCT = 264 reads: +382 validated
umi CTATGACGGC = 413 reads: +382 validated
umi CTCCAACCCA = 250 reads: +382 validated
umi CTCTGTGGAC = 405 reads: +382 validated
umi CTGTATTGCT = 333 reads: +382 validated
umi CTTCACTTTC = 152 reads: +382 validated
umi CTTGATTAGT = 288 reads: +382 validated
umi CTTGGTATAT = 276 reads: +382 validated
umi CTTTTCAGGG = 327 reads: +382 validated
umi GATGATATGC = 228 reads: +382 validated
umi GCAACCCACT = 322 reads: +382 validated
umi GCACGCATCT = 266 reads: +382 validated
umi GCATAGGGCT = 321 reads: +382 validated
umi GCCCTACGTG = 321 reads: +382 validated
umi GCCGGTTGAG = 175 reads: +382 validated
umi GCGTCGCGGG = 152 reads: +382 validated
umi GCTCTCTACT = 299 reads: +382 validated
umi GCTCTTGTGG = 380 reads: +382 validated
umi GCTTCGATCC = 336 reads: +382 validated
umi GGCGTGCCCA = 373 reads: +382 validated
umi GGCTTATGGC = 244 reads: +382 validated
umi GGGTGTGGGT = 668 reads: +382 validated
umi GGTTGTCAAA = 391 reads: +382 validated
umi GGTTTACCCT = 392 reads: +382 validated
umi GTATCATTTA = 347 reads: +382 validated
umi GTCAACGAGC = 280 reads: +382 validated
umi GTCGGAGTAT = 324 reads: +382 validated
umi GTGGTAATGT = 293 reads: +382 validated
umi GTTGTCTGCT = 365 reads: +382 validated
umi TAAGACCCGC = 360 reads: +382 validated
umi TACATTCCTC = 342 reads: +382 validated
umi TACGTACGCC = 162 reads: +382 validated
umi TAGAGACTTT = 290 reads: +382 validated
umi TAGGGTGACA = 247 reads: +382 validated
umi TAGTGCCGAG = 296 reads: +382 validated
umi TATCTATTAT = 405 reads: +382 validated
umi TCACGGTTAG = 305 reads: +382 validated
umi TCCCAGGATC = 254 reads: +382 validated
umi TCCTCGGCTT = 132 reads: +382 validated
umi TCGACCTATT = 574 reads: +382 validated
umi TCGCCCGACA = 335 reads: +382 validated
umi TCGCTCTCTC = 483 reads: +143 -4XX +1 -10XX +1 -3XX +3 -6XX +1 -210 invalidated
umi TCTAACTGCG = 356 reads: +382 validated
umi TCTCGCCCGA = 311 reads: +382 validated
umi TCTTCCACCT = 338 reads: +382 validated
umi TGACTTTTTA = 427 reads: +382 validated
umi TGCTCTCACG = 273 reads: +382 validated
umi TGTAACCTCA = 292 reads: +382 validated
umi TGTATCATCG = 226 reads: +382 validated
umi TGTCATTTCA = 228 reads: +382 validated
umi TTACCATTCA = 138 reads: +382 validated
umi TTATCGTAAT = 174 reads: +382 validated
umi TTCGTACCGA = 348 reads: +382 validated
umi TTCTATAGGG = 384 reads: +382 validated
umi TTCTCATACC = 221 reads: +382 validated
umi TTGGCCGTAA = 362 reads: +382 validated
umi TTTACTAAGC = 211 reads: +382 validated
umi TTTCCCCTAT = 312 reads: +382 validated
umi TTTCCGTAAA = 727 reads: +382 validated
umi TTTTAGGGTG = 121 reads: +382 validated
umi TTTTCCATCA = 380 reads: +382 validated
umi TTTTCTTTCC = 387 reads: +382 validated

UMI info for barcode AGATCTGTCCAGATCA-1 contig 2 = GAGCTCTGGG...
umi AACTCCTTCT = 186 reads: +386 -5 +42 non-validated
umi ACGTCAACAT = 122 reads: -392 +41 non-validated
umi ATTCTTTGCC = 59 reads: +433 validated
umi CCACTATCTC = 288 reads: +433 validated
umi CGCTTGCGCC = 338 reads: +433 validated
umi CGTCCGTTTG = 105 reads: +410 -23 non-validated
umi CTTATTTGGT = 77 reads: +424 -1 +8 non-validated
umi GCACTGCATT = 278 reads: +420 -13 non-validated

GOOD CONTIGS

TIG 1[bases=565]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=7)
392-429 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 112 umis using 5870 reads
cdr3 = CQQRKSWPLTF at 368, score = 9 + 9
umis assigned: [14, 20, 27, 31, 59, 70, 77, 78, 84, 94] and 104 others
of which 114 are surviving nonsolos
reads assigned: 35887
start codons at 47, 252, 255, 471
confident = true

TIG 2[bases=584]
0-80 ==> 0-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=19)
462-513 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=3)
513-584 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 4 umis using 49 reads
cdr3 = CAKDFPPRAAAGTGNWFDPW at 422, score = 8 + 6
umis assigned: [50, 218, 447, 638, 819, 864, 1007, 1102]
of which 8 are surviving nonsolos
reads assigned: 1419
start codons at 80, 231, 236, 294, 297, 315, 383
confident = true
now this is a cell
paired!

GTATATTACTGTGCGAAAGATTTTCCACCCCGGGCGGCAGCTGGAACGGGTAACTGGTTCGACCCCTGGGGCCAGGGAAACCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAAGAGTTGGCCTCTCACCTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.120 = AGATCTGTCCCTTGCA-1

using 2893 reads

====================================================================================

graph has 2062 edges initially, 46 edges after simplification

total ucounts = 415
nonsolo ucounts = 235[2^59, 3^44, 4^28, 5^28, 6^13, 7^11, 8^7, 9^5, 10^8, 11^5, 12^3, 13^6, 14, 15^3, 16, 17, 20^2, 21, 34, 41, 63, 65, 241, 247, 275, 292, 295]
surviving nonsolo ucounts = 8[41, 63, 65, 241, 247, 275, 292, 295]
ids = [273, 93, 264, 111, 233, 12, 6, 37]

====================================================================================

UMI info for barcode AGATCTGTCCCTTGCA-1 contig 1 = CTGGGAGAGG...
umi AACCTACCAT = 273 reads: +424 validated
umi AATAGACTGT = 268 reads: +424 validated
umi ACTGCATTTC = 294 reads: +413 -11 non-validated
umi CACTTGGCCA = 63 reads: +339 -1 +73 -1 +3 -1 +6 non-validated

UMI info for barcode AGATCTGTCCCTTGCA-1 contig 2 = GGAGAAGAGC...
umi CCAAGCGAGT = 241 reads: +385 validated
umi GCCCTTCTAA = 249 reads: +385 validated
umi GGGGCATTGG = 57 reads: -385 non-validated

GOOD CONTIGS

TIG 1[bases=533]
0-75 ==> 146-221 on |145|IGHV3-53|5'UTR| [len=221] (mis=0)
75-425 ==> 0-350 on |146|IGHV3-53|L-REGION+V-REGION| [len=350] (mis=4)
436-499 ==> 0-63 on |737|IGHJ6|J-REGION| [len=63] (mis=5)
499-533 ==> 0-34 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 2 umis using 34 reads
cdr3 = CARGLLPGIIYYYGMDVW at 414, score = 9 + 7
umis assigned: [6, 12, 37, 93]
of which 4 are surviving nonsolos
reads assigned: 888
start codons at 75, 231, 375, 456, 517
confident = true

TIG 2[bases=557]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=12)
383-421 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 74 reads
cdr3 = CQQYGHSLFTF at 360, score = 9 + 8
umis assigned: [111, 233, 264]
of which 3 are surviving nonsolos
reads assigned: 539
start codons at 36, 244, 370, 463
confident = true
now this is a cell
paired!

ACGGCCGTGTATTACTGTGCGAGAGGATTACTCCCTGGTATCATCTACTACTACGGTATGGACGTCTGGGGCCAAGGGACCACGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTCACTCTCTATTCACTTTCGGCCCTGGGACCAAAGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.121 = AGATCTGTCCGCGCAA-1

using 865 reads

====================================================================================

graph has 355 edges initially, 8 edges after simplification

total ucounts = 15
nonsolo ucounts = 9[2, 3, 7^2, 8, 9, 211, 300, 312]
surviving nonsolo ucounts = 3[211, 300, 312]
ids = [3, 12, 9]

====================================================================================

UMI info for barcode AGATCTGTCCGCGCAA-1 contig 1 = GCTTCAGCTG...
umi CAAAGTATAG = 207 reads: +394 validated

UMI info for barcode AGATCTGTCCGCGCAA-1 contig 2 = AGAGCTCTGG...
umi TAATCTCAGT = 299 reads: +385 validated

UMI info for barcode AGATCTGTCCGCGCAA-1 contig 3 = GTCAGACCCT...
umi CTCGCCCTAC = 314 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=526]
0-46 ==> 5-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
46-402 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
402-440 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
440-526 ==> 0-86 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CQSYDSSLSGLYVF at 370, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 202
start codons at 46, 200, 203, 254, 353, 380, 404
confident = false

TIG 2[bases=565]
0-44 ==> 8-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
44-392 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
390-429 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
429-565 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQYGSSPWTF at 368, score = 9 + 8
umis assigned: [12]
of which 1 are surviving nonsolos
reads assigned: 298
start codons at 44, 252, 378, 471
confident = false

TIG 3[bases=547]
0-29 ==> 24-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=0)
29-374 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=1)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 64 reads
cdr3 = CQQYYSYPWTF at 350, score = 9 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.124 = AGATCTGTCCGTCAAA-1

using 785 reads

====================================================================================

graph has 688 edges initially, 12 edges after simplification

total ucounts = 215
nonsolo ucounts = 117[2^31, 3^23, 4^5, 5^8, 6^12, 7^4, 8^5, 9^2, 10^8, 11^2, 12^4, 13^5, 14^2, 15^4, 16, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.133 = AGATCTGTCGGAAATA-1

using 329 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 6, 317]
surviving nonsolo ucounts = 1[317]
ids = [1]

====================================================================================

UMI info for barcode AGATCTGTCGGAAATA-1 contig 1 = AGGAGTCAGA...
umi ATCAATCCGC = 314 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=551]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=25)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQGNSFPYTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 27, 33, 89, 102, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.134 = AGATCTGTCGGTGTCG-1

using 909 reads

====================================================================================

graph has 358 edges initially, 12 edges after simplification

total ucounts = 8
nonsolo ucounts = 6[2, 4^2, 11, 203, 683]
surviving nonsolo ucounts = 2[203, 683]
ids = [0, 4]

====================================================================================

UMI info for barcode AGATCTGTCGGTGTCG-1 contig 1 = AGTCCCAACC...
umi GGCTTTCGCC = 683 reads: +385 validated

UMI info for barcode AGATCTGTCGGTGTCG-1 contig 2 = GGGGAGGAAC...
umi AAGAGGCTTT = 205 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=541]
0-20 ==> 11-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
20-371 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=3)
367-405 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
405-541 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 105 reads
cdr3 = CQQYDNLPTF at 347, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 669
start codons at 20, 26, 82, 95, 234, 357, 447
confident = false

TIG 2[bases=554]
0-36 ==> 61-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
36-381 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=14)
380-418 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CHQYDNWPQTF at 357, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 201
start codons at 36, 85, 105, 241, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.135 = AGATCTGTCGTCTGAA-1

using 105 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[102]
surviving nonsolo ucounts = 1[102]
ids = [2]

====================================================================================

UMI info for barcode AGATCTGTCGTCTGAA-1 contig 1 = ACCCAAAAAC...
umi GATCCGCTCT = 103 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=531]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-531 ==> 0-41 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 8 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 101
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.157 = AGATTGCAGGACAGCT-1

using 399 reads

====================================================================================

graph has 174 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[3, 389]
surviving nonsolo ucounts = 1[389]
ids = [3]

====================================================================================

UMI info for barcode AGATTGCAGGACAGCT-1 contig 1 = GAATCAGTCC...
umi CCATGCCTGG = 367 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=481]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-481 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 63 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 355
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.160 = AGATTGCAGGCTACGA-1

using 220 reads

====================================================================================

graph has 106 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 8, 204]
surviving nonsolo ucounts = 2[8, 204]
ids = [0, 3]

====================================================================================

UMI info for barcode AGATTGCAGGCTACGA-1 contig 1 = GGGACTGATC...
umi ATTTTTCGCG = 8 reads: -15 +4 -1 +2 -1 +4 -2 +75 -43 +126 -65 +20 -5X +1 -2X +2 -1X +5 -1X +5 -4X +8 -1X +1 -3 invalidated
umi TACGAAGAGC = 193 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=461]
0-36 ==> 0-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=2)
36-396 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=2)
395-433 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
433-461 ==> 0-28 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 26 reads
cdr3 = CMQALQTRETF at 372, score = 9 + 8
umis assigned: [0, 3]
of which 2 are surviving nonsolos
reads assigned: 198
start codons at 36, 69, 105, 193, 355, 375
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.169 = AGATTGCAGTAGGCCA-1

using 609 reads

====================================================================================

graph has 494 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[5, 271, 327]
surviving nonsolo ucounts = 2[271, 327]
ids = [4, 2]

====================================================================================

UMI info for barcode AGATTGCAGTAGGCCA-1 contig 1 = GTCAGACTCA...
umi GTTTGCCGCT = 277 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=27)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=6)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQYNGQSRAF at 350, score = 8 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 271
start codons at 23, 29, 98, 234, 237, 330, 363, 453
confident = false

REJECT CONTIGS

TIG 1[bases=541]
0-79 ==> 0-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
0-79 ==> 5921-6000 on rc of segment after IGHV3-7 exon 1 [len=6000] (mis=0)
47-118 ==> 6506-6577 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=4)
79-291 ==> 0-212 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=4)
291-364 ==> 280-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=4) [SHIFT!]
383-429 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
429-541 ==> 0-112 on |40|IGHG1|C-REGION| [len=884] (mis=1)
cdr3 = CARDTYYYGSGVDYW at 353, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 301
start codons at 79, 235, 314, 375
confident = false
frameshifted full length transcript of length 541
VJ delta = 74
delta too large
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.170 = AGATTGCAGTAGGTGC-1

using 814 reads

====================================================================================

graph has 306 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[4, 293, 516]
surviving nonsolo ucounts = 2[293, 516]
ids = [2, 0]

====================================================================================

UMI info for barcode AGATTGCAGTAGGTGC-1 contig 1 = GTGCAGGAGT...
umi AGGAGTCAGT = 446 reads: +388 validated
umi CGTCTCCGCT = 264 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=512]
0-31 ==> 16-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
31-382 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
381-419 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
419-512 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 114 reads
cdr3 = CQQSYRRPITF at 358, score = 8 + 8
umis assigned: [0, 2]
of which 2 are surviving nonsolos
reads assigned: 699
start codons at 31, 37, 93, 106, 242, 341, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.181 = AGATTGCCAAATCCGT-1

using 1161 reads

====================================================================================

graph has 1881 edges initially, 2 edges after simplification

total ucounts = 629
nonsolo ucounts = 240[2^119, 3^49, 4^33, 5^19, 6^10, 7^5, 8, 10, 12, 13, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.188 = AGATTGCCACATGTGT-1

using 338 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 333]
surviving nonsolo ucounts = 1[333]
ids = [0]

====================================================================================

UMI info for barcode AGATTGCCACATGTGT-1 contig 1 = GGAGTCAGAC...
umi ACCAGTCTCG = 326 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=547]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=19)
373-411 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CQQSNSYWTF at 353, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 323
start codons at 26, 32, 86, 101, 237, 240, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.193 = AGATTGCCACGAGAGT-1

using 280 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 4, 270]
surviving nonsolo ucounts = 1[270]
ids = [3]

====================================================================================

UMI info for barcode AGATTGCCACGAGAGT-1 contig 1 = CTGCTCAGTT...
umi CCGATTACAT = 254 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=473]
0-27 ==> 25-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
27-375 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
373-412 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
412-473 ==> 0-61 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CQHYGNSPGTF at 351, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 249
start codons at 27, 235, 361, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.195 = AGATTGCCACTATCTT-1

using 96 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 90]
surviving nonsolo ucounts = 1[90]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.197 = AGATTGCCAGCTGCTG-1

using 297 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[295]
surviving nonsolo ucounts = 1[295]
ids = [0]

====================================================================================

UMI info for barcode AGATTGCCAGCTGCTG-1 contig 1 = ATCAGACCCA...
umi CACTGTGTAC = 276 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=506]
0-23 ==> 4-27 on |220|IGKV1-16|5'UTR| [len=27] (mis=0)
23-374 ==> 0-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=6)
379-411 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-506 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQYNSYPPTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 274
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.198 = AGATTGCCAGCTTCGG-1

using 261 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[5, 10, 239]
surviving nonsolo ucounts = 1[239]
ids = [4]

====================================================================================

UMI info for barcode AGATTGCCAGCTTCGG-1 contig 1 = GCTCTGCTTC...
umi CAGACGGCCG = 227 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=544]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-544 ==> 0-99 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 223
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.200 = AGATTGCCAGGCTGAA-1

using 362 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2, 6, 9, 343]
surviving nonsolo ucounts = 1[343]
ids = [1]

====================================================================================

UMI info for barcode AGATTGCCAGGCTGAA-1 contig 1 = AGTCCCAACC...
umi CGGAGCCTAG = 341 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=544]
0-20 ==> 11-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
20-371 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=6)
371-408 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
408-544 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYDFLPLTF at 347, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 337
start codons at 20, 26, 82, 95, 234, 357, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.202 = AGATTGCCATCACGTA-1

using 93 reads

====================================================================================

graph has 76 edges initially, 6 edges after simplification

total ucounts = 23
nonsolo ucounts = 16[2^5, 3^2, 4, 6, 7^4, 9^2, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.204 = AGATTGCCATCGGAAG-1

using 242 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 238]
surviving nonsolo ucounts = 1[238]
ids = [2]

====================================================================================

UMI info for barcode AGATTGCCATCGGAAG-1 contig 1 = TGGGGTCACA...
umi CCACAGCTAT = 2 reads: -104 +3 -2 +10 -1 +2 -1 +1 -7X +7 -1 +2 -1 +12 -3X +1 -1 +1 -234 invalidated
umi CCACAGCTTG = 226 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=544]
0-39 ==> 123-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
39-379 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=5)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
433-544 ==> 0-111 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CCSYAGSRTPNWVF at 363, score = 8 + 8
umis assigned: [1, 2]
of which 1 are surviving nonsolos
reads assigned: 226
start codons at 39, 178, 240, 247, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.206 = AGATTGCCATCTCGCT-1

using 1293 reads

====================================================================================

graph has 1880 edges initially, 6 edges after simplification

total ucounts = 672
nonsolo ucounts = 259[2^105, 3^64, 4^39, 5^25, 6^11, 7^5, 8^5, 9, 10, 11, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.210 = AGATTGCCATTAGGCT-1

using 291 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[290]
surviving nonsolo ucounts = 1[290]
ids = [1]

====================================================================================

UMI info for barcode AGATTGCCATTAGGCT-1 contig 1 = AGGAGGCAGC...
umi TCTGGTGTTA = 287 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=540]
30-374 ==> 0-344 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=20)
380-418 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
418-540 ==> 0-122 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CSSYISSTTLGF at 354, score = 8 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 285
start codons at 30, 238, 241
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.212 = AGATTGCGTAAGGGAA-1

using 84 reads

====================================================================================

graph has 74 edges initially, 6 edges after simplification

total ucounts = 13
nonsolo ucounts = 10[3^3, 5, 6, 7, 8, 12^2, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.219 = AGATTGCGTCAATACC-1

using 284 reads

====================================================================================

graph has 138 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[7, 276]
surviving nonsolo ucounts = 1[276]
ids = [2]

====================================================================================

UMI info for barcode AGATTGCGTCAATACC-1 contig 1 = GGGAGTCTCA...
umi GCTTTACCAA = 276 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=550]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=2)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=1)
375-414 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQSYSTPLVTF at 350, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 23, 29, 85, 98, 234, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.224 = AGATTGCGTCGCGGTT-1

using 23 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.229 = AGATTGCGTGAAATCA-1

using 748 reads

====================================================================================

graph has 352 edges initially, 6 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[3, 214, 261, 267]
surviving nonsolo ucounts = 3[214, 261, 267]
ids = [4, 6, 3]

====================================================================================

UMI info for barcode AGATTGCGTGAAATCA-1 contig 1 = GAGCCCCAGC...
umi GCACGCCTGC = 263 reads: +409 validated

UMI info for barcode AGATTGCGTGAAATCA-1 contig 2 = GCTCTGCTTC...
umi GTAACAATCG = 199 reads: +391 validated

UMI info for barcode AGATTGCGTGAAATCA-1 contig 3 = GTCAGTCTCA...
umi GTTTCTTGTT = 249 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=574]
0-67 ==> 169-236 on |168|IGHV3-74|5'UTR| [len=236] (mis=0)
67-398 ==> 0-331 on |169|IGHV3-74|L-REGION+V-REGION| [len=351] (mis=17)
439-476 ==> 26-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
476-574 ==> 0-98 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 31 reads
cdr3 = CTNLYHFGSEVW at 409, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 67, 223, 284, 530
confident = false

TIG 2[bases=531]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=10)
404-442 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
442-531 ==> 0-89 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQSYDINLIGVIF at 375, score = 7 + 9
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 196
start codons at 51, 114, 205, 208, 259, 358, 385
confident = false

TIG 3[bases=466]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=8)
376-414 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
414-466 ==> 0-52 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQSYSTPQITF at 350, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 245
start codons at 23, 29, 85, 98, 234, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 9.236 = AGATTGCGTTCAGACT-1

using 490 reads

====================================================================================

graph has 178 edges initially, 6 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[5, 225, 257]
surviving nonsolo ucounts = 2[225, 257]
ids = [1, 5]

====================================================================================

UMI info for barcode AGATTGCGTTCAGACT-1 contig 1 = GGTGGTAGCT...
umi TCGTGCCAAT = 251 reads: +391 validated

UMI info for barcode AGATTGCGTTCAGACT-1 contig 2 = AGCTGGGAAG...
umi CTAACGTCTT = 222 reads: +403 validated

GOOD CONTIGS

TIG 1[bases=584]
0-39 ==> 47-86 on |732|IGLV6-57|5'UTR| [len=86] (mis=0)
39-362 ==> 0-323 on |384|IGLV6-57|L-REGION+V-REGION| [len=353] (mis=16)
392-430 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
430-584 ==> 0-154 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQSSDNSNPWVF at 366, score = 6 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 246
start codons at 39, 193, 244
confident = false

TIG 2[bases=587]
0-30 ==> 16-46 on |389|IGLV8-61|5'UTR| [len=46] (mis=0)
30-398 ==> 0-368 on |390|IGLV8-61|L-REGION+V-REGION| [len=368] (mis=3)
395-433 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
433-587 ==> 0-154 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CVLYMGSGIWVF at 369, score = 7 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 220
start codons at 30, 45, 54, 57, 82, 349, 352, 381
confident = false
NOT paired!
sorting bam, mem = 0.08
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk009-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk009-ucc6a7a72ce/files/contig_bam.bam

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

16.872 seconds used processing barcodes, peak mem = 0.25
