[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.37 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk007-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk007-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk007.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.0 = ACCGTAATCAGCAACT-1

using 5587 reads

====================================================================================

graph has 2926 edges initially, 70 edges after simplification

total ucounts = 583
nonsolo ucounts = 228[2^98, 3^58, 4^14, 5^12, 6^8, 7^6, 8^5, 9^3, 11, 12^2, 13, 17, 23, 91, 94, 146, 162, 207, 217, 230, 235, 238, 274, 281, 283, 302, 320, 331, 332, 354, 404]
surviving nonsolo ucounts = 17[91, 94, 146, 162, 217, 230, 235, 238, 274, 281, 283, 302, 320, 331, 332, 354, 404]
ids = [145, 323, 300, 230, 137, 104, 379, 219, 380, 571, ...]

====================================================================================

UMI info for barcode ACCGTAATCAGCAACT-1 contig 1 = GGAGTCAGTC...
umi AGCGGTAGAT = 229 reads: +388 validated
umi AGGACTGGCT = 322 reads: +388 validated
umi ATGACTAGTA = 90 reads: +388 validated
umi CCAGGGATCT = 240 reads: +388 validated
umi CCCTTTTGGA = 165 reads: +53 -1XX +334 invalidated
umi TTATTAGGGA = 332 reads: +388 validated

UMI info for barcode ACCGTAATCAGCAACT-1 contig 2 = AGGTCTCAGA...
umi ATCCCAGGAT = 360 reads: +406 validated
umi CAGACTATCG = 303 reads: +406 validated
umi GACATGGCAG = 80 reads: +324 -1 +3 -2 +11 -1 +1 -1 +2 -1 +1 -2 +2 -1 +2 -2 +3 -1 +1 -1 +3 -1 +1 -1 +3 -34 non-validated
umi GCCTCACAAG = 38 reads: -350X +1 -1X +1 -2X +1 -1X +1 -5X +2 -2XX +1 -2XX +13 -1XX +22 invalidated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 5-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
26-377 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
376-414 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 6 umis using 229 reads
cdr3 = CQQYDNLPFTF at 353, score = 9 + 8
umis assigned: [104, 107, 145, 219, 230, 551]
of which 6 are surviving nonsolos
reads assigned: 1356
start codons at 26, 32, 88, 101, 240, 363, 456
confident = true

TIG 2[bases=556]
0-79 ==> 0-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=4)
440-485 ==> 7-52 on |49|IGHJ1|J-REGION| [len=52] (mis=0)
485-556 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 2 umis using 73 reads
cdr3 = CARGYFNFQHW at 421, score = 9 + 7
umis assigned: [138, 186, 323, 364]
of which 3 are surviving nonsolos
reads assigned: 769
start codons at 79, 235, 296, 314, 382
confident = true

REJECT CONTIGS

TIG 1[bases=551]
9-79 ==> 5667-5737 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=1)
377-415 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=4)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [28, 137, 300, 326, 374, 379, 380, 571]
of which 8 are surviving nonsolos
reads assigned: 2090
start codons at 31, 37, 106, 188, 242, 457
confident = false
did not find CDR3
now this is a cell
paired!

AACAGCCTGAGAGCCGAGGACACGGCTGTGTATTACTGTGCGAGGGGGTATTTTAACTTCCAGCACTGGGGCCAGGGCACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGCCTGAAGATATTGCAACATATTACTGTCAACAGTATGATAATCTCCCTTTCACTTTCGGCCCTGGGACCAAAGTGGATATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.6 = ACCGTAATCCACGTTC-1

using 207 reads

====================================================================================

graph has 116 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 5, 194]
surviving nonsolo ucounts = 1[194]
ids = [0]

====================================================================================

UMI info for barcode ACCGTAATCCACGTTC-1 contig 1 = GAGTCAGTCT...
umi AAGGGCCTTA = 195 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=546]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=5)
372-410 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
410-546 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQQSYSSITF at 352, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 192
start codons at 25, 31, 87, 100, 236, 452
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.25 = ACCGTAATCTAACTGG-1

using 224 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 221]
surviving nonsolo ucounts = 1[221]
ids = [2]

====================================================================================

UMI info for barcode ACCGTAATCTAACTGG-1 contig 1 = GCTCTGCTTC...
umi GTGTTAAGGC = 212 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=471]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=22)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=6)
442-471 ==> 0-29 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQSYDSILTGWAF at 375, score = 7 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 209
start codons at 51, 205, 358, 385
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.34 = ACCGTAATCTGGGCCA-1

using 238 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[3, 5, 228]
surviving nonsolo ucounts = 1[228]
ids = [3]

====================================================================================

UMI info for barcode ACCGTAATCTGGGCCA-1 contig 1 = TGAGCGCAGA...
umi TCATGACCTA = 220 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=535]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-389 ==> 0-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=13)
386-424 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=4)
424-535 ==> 0-111 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CGTWDASLSAGVF at 357, score = 7 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 215
start codons at 36, 190, 237, 241, 247, 365, 370
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.52 = ACCTTTAAGACTGGGT-1

using 49 reads

====================================================================================

graph has 56 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 7[2^2, 3, 6, 8^2, 16]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.55 = ACCTTTAAGAGCCTAG-1

using 6026 reads

====================================================================================

graph has 4607 edges initially, 118 edges after simplification

total ucounts = 1399
nonsolo ucounts = 943[2^181, 3^150, 4^98, 5^113, 6^81, 7^67, 8^53, 9^51, 10^36, 11^19, 12^20, 13^30, 14^12, 15^10, 16^4, 17^2, 18^4, 19^4, 20, 21^4, 22, 23, 50]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.60 = ACCTTTAAGAGGTTAT-1

using 367 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2^2, 4, 356]
surviving nonsolo ucounts = 1[356]
ids = [6]

====================================================================================

REJECT CONTIGS

TIG 1[bases=487]
1-314 ==> 38-351 on |221|IGKV1-16|L-REGION+V-REGION| [len=351] (mis=6)
319-351 ==> 7-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
351-487 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYNSYPPTF at 290, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 347
start codons at 25, 38, 174, 393
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.63 = ACCTTTAAGAGTACAT-1

using 1850 reads

====================================================================================

graph has 1909 edges initially, 24 edges after simplification

total ucounts = 744
nonsolo ucounts = 382[2^140, 3^88, 4^57, 5^27, 6^20, 7^16, 8^11, 9^3, 10^6, 11^6, 12^3, 13^2, 14, 15, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.64 = ACCTTTAAGATAGTCA-1

using 306 reads

====================================================================================

graph has 136 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 11[2, 3^3, 5^2, 9, 11^2, 12, 239]
surviving nonsolo ucounts = 1[239]
ids = [5]

====================================================================================

UMI info for barcode ACCTTTAAGATAGTCA-1 contig 1 = AGGAACTGCT...
umi CCCGTGCGCG = 226 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=497]
0-32 ==> 15-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
32-377 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=1)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
414-497 ==> 0-83 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQRSNWPPTF at 353, score = 9 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 32, 237, 240, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.65 = ACCTTTAAGATCACGG-1

using 29 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 5[2, 3, 4, 6^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.84 = ACCTTTAAGCTGCAAG-1

using 786 reads

====================================================================================

graph has 1024 edges initially, 10 edges after simplification

total ucounts = 356
nonsolo ucounts = 157[2^60, 3^35, 4^22, 5^14, 6^10, 7^5, 8^5, 9, 10^2, 11, 12, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.91 = ACCTTTAAGGGTCGAT-1

using 68 reads

====================================================================================

graph has 80 edges initially, 2 edges after simplification

total ucounts = 24
nonsolo ucounts = 14[2^5, 3^5, 5, 6, 9, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.92 = ACCTTTAAGTACACCT-1

using 288 reads

====================================================================================

graph has 132 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2^2, 8, 275]
surviving nonsolo ucounts = 1[275]
ids = [3]

====================================================================================

UMI info for barcode ACCTTTAAGTACACCT-1 contig 1 = TGGGGAGGAA...
umi GGACCATGGC = 267 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=496]
0-37 ==> 10-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
37-382 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
419-496 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQRSNWPLTF at 358, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 262
start codons at 37, 245, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.104 = ACCTTTAAGTTCCACA-1

using 1496 reads

====================================================================================

graph has 1987 edges initially, 16 edges after simplification

total ucounts = 682
nonsolo ucounts = 308[2^126, 3^79, 4^30, 5^22, 6^22, 7^7, 8^6, 9^6, 10^3, 11^4, 13, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.108 = ACCTTTACAAAGCAAT-1

using 504 reads

====================================================================================

graph has 246 edges initially, 4 edges after simplification

total ucounts = 14
nonsolo ucounts = 5[2, 3, 5, 229, 256]
surviving nonsolo ucounts = 2[229, 256]
ids = [4, 11]

====================================================================================

UMI info for barcode ACCTTTACAAAGCAAT-1 contig 1 = AGGAGTCAGT...
umi TCGTTTTACT = 242 reads: +388 validated

UMI info for barcode ACCTTTACAAAGCAAT-1 contig 2 = TGGGGGCTTT...
umi GCCATATCAC = 225 reads: +451 validated

GOOD CONTIGS

TIG 1[bases=503]
27-380 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
415-503 ==> 0-88 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 35 reads
cdr3 = CQQSYTTLLIF at 354, score = 9 + 9
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 238
start codons at 27, 33, 89, 102, 238, 457
confident = false

TIG 2[bases=549]
40-385 ==> 0-345 on |742|IGHV4-38-2|L-REGION+V-REGION| [len=345] (mis=10)
439-491 ==> 0-52 on |49|IGHJ1|J-REGION| [len=52] (mis=2)
491-549 ==> 0-58 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARAPIHAPKGWPSAPGYRAEYFHHW at 382, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 221
start codons at 19, 40, 84, 188, 304, 401, 414
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.112 = ACCTTTACAACACCTA-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[28]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.126 = ACCTTTACACAGGAGT-1

using 31 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[2^2, 3, 4^2, 5^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.130 = ACCTTTACACATAACC-1

using 365 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2, 3, 7, 348]
surviving nonsolo ucounts = 1[348]
ids = [3]

====================================================================================

UMI info for barcode ACCTTTACACATAACC-1 contig 1 = GGAACTGCTC...
umi ATATTATCCT = 351 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=552]
0-31 ==> 66-97 on |291|IGKV3D-20|5'UTR| [len=97] (mis=0)
31-379 ==> 0-348 on |292|IGKV3D-20|L-REGION+V-REGION| [len=348] (mis=1)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 62 reads
cdr3 = CQQYGSSPWTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 346
start codons at 31, 239, 242, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.139 = ACCTTTACAGAAGCAC-1

using 398 reads

====================================================================================

graph has 166 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 6[2, 3, 4, 10, 123, 250]
surviving nonsolo ucounts = 3[10, 123, 250]
ids = [9, 11, 10]

====================================================================================

UMI info for barcode ACCTTTACAGAAGCAC-1 contig 1 = GACTCCTGTG...
umi TCAACCAGGG = 10 reads: -4 +102 -1 +17 -31 +41 -1X +14 -40 +4 -1 +3 -1 +47 -63 +63 invalidated
umi TGCGTCTGGG = 252 reads: +433 validated

GOOD CONTIGS

TIG 1[bases=502]
17-375 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=20)
400-450 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=4)
450-502 ==> 0-52 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CAHQLAIVTGYYENAFDIW at 362, score = 8 + 8
umis assigned: [9, 10]
of which 2 are surviving nonsolos
reads assigned: 253
start codons at 17, 61, 240, 243, 246, 332, 402
confident = false

REJECT CONTIGS

TIG 1[bases=406]
0-52 ==> 0-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-52 ==> 5948-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
52-400 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=4)
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 118
start codons at 52, 260, 386
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.143 = ACCTTTACAGCCAATT-1

using 370 reads

====================================================================================

graph has 186 edges initially, 6 edges after simplification

total ucounts = 16
nonsolo ucounts = 7[2^5, 146, 205]
surviving nonsolo ucounts = 2[146, 205]
ids = [14, 10]

====================================================================================

UMI info for barcode ACCTTTACAGCCAATT-1 contig 1 = ACCCAAAAAC...
umi TAAATACCTT = 203 reads: +436 validated
umi TTAGCATTAC = 148 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=552]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-552 ==> 0-62 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 2 umis using 31 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [10, 14]
of which 2 are surviving nonsolos
reads assigned: 341
start codons at 54, 252, 257, 274, 318, 351, 544
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.149 = ACCTTTACAGCTGTTA-1

using 38564 reads

====================================================================================

graph has 9464 edges initially, 96 edges after simplification

total ucounts = 815
nonsolo ucounts = 476[2^137, 3^82, 4^40, 5^30, 6^26, 7^6, 8^8, 9^8, 10^4, 11^2, 12^4, 14^2, 18, 23, 26, 30, 37, 38, 46, 80, 89, 93, 107, 114^2, 130, 133, 154, 161, 179, 183, 184, 186, 188, 198, 204, 205, 208, 212, 220^2, 224, 227, 230, 240, 243, 244, 245, 248, 250, 251, 252, 256, 257, 258, 262^2, 268, 271, 272, 274, 276, 278^2, 279, 280, 281^3, 285, 287, 291^2, 292^2, 294, 295, 296, 299^2, 300, 302, 303^3, 304, 305, 307, 309, 311, 312, 314, 315, 316, 317, 320, 322, 323, 325, 327, 328, 329^2, 330, 331, 332, 335, 340, 341, 342, 343, 346^2, 349, 351, 352, 353, 354, 355, 359, 360^2, 365, 367, 370, 372, 373, 378, 421, 426, 466, 528, 574, 611^2, 635, 691, 698, 964]
surviving nonsolo ucounts = 118[80, 89, 107, 114^2, 130, 133, 154, 161, 183, 184, 186, 188, 198, 204, 205, 208, 212, 220^2, 224, 227, 230, 240, 243, 244, 245, 248, 250, 251, 252, 256, 257, 258, 262^2, 268, 271, 272, 274, 276, 278^2, 279, 280, 281^3, 285, 287, 291^2, 292^2, 294, 295, 296, 299^2, 300, 302, 303^3, 304, 305, 307, 309, 311, 312, 314, 315, 316, 317, 320, 322, 323, 325, 327, 328, 329^2, 330, 331, 332, 335, 340, 341, 342, 343, 346^2, 349, 351, 352, 353, 354, 355, 359, 360^2, 365, 367, 370, 372, 373, 378, 421, 426, 466, 528, 574, 611^2, 635, 691, 698, 964]
ids = [279, 688, 137, 305, 506, 349, 472, 521, 733, 204, ...]

====================================================================================

UMI info for barcode ACCTTTACAGCTGTTA-1 contig 1 = GGGAGAGCCC...
umi AACACTTTGC = 273 reads: +385 validated
umi AACGACTCCC = 305 reads: +385 validated
umi AAGGTACTTT = 251 reads: +385 validated
umi AATGATCCTG = 474 reads: +385 validated
umi AATTTGCGAG = 362 reads: +385 validated
umi ACATCGCGCA = 277 reads: +385 validated
umi ACCGTGCAGT = 355 reads: +385 validated
umi ACGAATCTTT = 287 reads: +385 validated
umi ACGTTTCTTA = 238 reads: +385 validated
umi ACTGTAGACG = 199 reads: +385 validated
umi ACTTTACATT = 326 reads: +385 validated
umi AGGTCGCGGG = 303 reads: +385 validated
umi ATAAGATCGC = 185 reads: +385 validated
umi ATAAGCAAAC = 445 reads: -351X +2 -1XX +6 -2XX +15 -1XX +7 invalidated
umi ATCAATGTCC = 322 reads: +385 validated
umi ATGCAAACAT = 282 reads: +184 -1XX +200 invalidated
umi ATGGCTCACT = 611 reads: +385 validated
umi ATGTTGGGGC = 298 reads: +385 validated
umi ATTGCCATCC = 300 reads: +385 validated
umi CACCACAACC = 247 reads: +385 validated
umi CACCTGAACC = 420 reads: +385 validated
umi CACGTCCGTG = 187 reads: +385 validated
umi CACTACACTT = 309 reads: +385 validated
umi CAGAATGGTT = 245 reads: +385 validated
umi CATACATTGT = 354 reads: +385 validated
umi CCACGTATCA = 316 reads: +385 validated
umi CCCAACGTCG = 240 reads: +385 validated
umi CGATACGCTC = 296 reads: +385 validated
umi CGCTTCCTCC = 253 reads: +385 validated
umi CGTCGTTGGG = 302 reads: +385 validated
umi CGTTTTTTAG = 732 reads: +385 validated
umi CTAAAAGCGA = 281 reads: +385 validated
umi CTCATCGCTG = 285 reads: +385 validated
umi CTCCGTTCAG = 688 reads: +385 validated
umi CTCTAGTCCG = 265 reads: +385 validated
umi CTGTTATCTG = 268 reads: +385 validated
umi CTTGGATCCG = 374 reads: +385 validated
umi CTTGTAATCG = 610 reads: +385 validated
umi GAATTATAGC = 370 reads: +385 validated
umi GACACTCACA = 206 reads: +385 validated
umi GAGCGAAGCT = 302 reads: +385 validated
umi GATACGCGCC = 350 reads: +385 validated
umi GCACTGCCTG = 323 reads: +385 validated
umi GCCCGTACGC = 257 reads: +385 validated
umi GCCTGTTACA = 229 reads: +385 validated
umi GCTCATATAT = 331 reads: +385 validated
umi GCTTTGCGCC = 342 reads: +385 validated
umi GGACCATGTG = 544 reads: +385 validated
umi GGATTGGTTC = 329 reads: +385 validated
umi GGCGGGAGGG = 279 reads: +385 validated
umi GGGCCGTACT = 363 reads: +385 validated
umi GGGTCGCCAA = 329 reads: +385 validated
umi GGTTCCGCAC = 302 reads: +385 validated
umi GTATAATTAC = 330 reads: +385 validated
umi GTTACTTCAT = 269 reads: +385 validated
umi TAAATACATC = 315 reads: +385 validated
umi TAAGTACTAC = 356 reads: +385 validated
umi TACTAATCGG = 332 reads: +385 validated
umi TACTCTGATG = 306 reads: +385 validated
umi TATAAAGGTT = 186 reads: +385 validated
umi TATTCGCAGG = 205 reads: +385 validated
umi TCAATATCAT = 365 reads: +385 validated
umi TCATGATTTG = 640 reads: +385 validated
umi TCCGCATTCT = 309 reads: +126 -1XX +258 invalidated
umi TCCGCGAAGG = 259 reads: +385 validated
umi TCCGGCCATG = 319 reads: +385 validated
umi TCCTGATCAG = 373 reads: +385 validated
umi TCCTTTTTTC = 338 reads: +385 validated
umi TCGAATCGGT = 340 reads: +385 validated
umi TCGCGATGCG = 345 reads: +385 validated
umi TCTGTAATTA = 354 reads: +385 validated
umi TCTTCCCATC = 374 reads: +385 validated
umi TCTTGCTATC = 344 reads: +385 validated
umi TGAATTATCT = 316 reads: +385 validated
umi TGCCCGACCT = 330 reads: +385 validated
umi TTACCAGACC = 322 reads: +385 validated
umi TTAGTTCAGT = 333 reads: +385 validated
umi TTCACTTCTT = 279 reads: +385 validated
umi TTCAGATTCA = 293 reads: +385 validated
umi TTCAGTCCCG = 276 reads: +385 validated
umi TTGCTTTCTC = 291 reads: +385 validated
umi TTTGTTACTA = 363 reads: +385 validated
umi TTTTTATACT = 304 reads: +385 validated
umi TTTTTGAACG = 278 reads: +385 validated

UMI info for barcode ACCTTTACAGCTGTTA-1 contig 2 = GGGGGACCCA...
umi ACGTACTTCT = 324 reads: +427 validated
umi ATTTTTTTCG = 229 reads: +427 validated
umi CAGGGTTACC = 220 reads: +427 validated
umi CATTCGCATC = 330 reads: +427 validated
umi CCCGTACATT = 214 reads: +427 validated
umi CCGAGACCGC = 286 reads: +427 validated
umi CCGCGTCCGA = 74 reads: +427 validated
umi CGCAACAGGG = 115 reads: +412 -15 non-validated
umi CTAAATGGCT = 132 reads: +427 validated
umi CTACCCGTCT = 239 reads: +427 validated
umi CTGCCGTTTT = 206 reads: +427 validated
umi CTTATACTCT = 279 reads: +427 validated
umi GACTAGGCAC = 283 reads: +427 validated
umi GCTTAAAGCT = 164 reads: +427 validated
umi GGAGACATCG = 103 reads: +412 -1 +1 -13 non-validated
umi GGTATACGGG = 155 reads: +419 -8 non-validated
umi GGTCTGCTTC = 423 reads: +427 validated
umi GTGCTCCCTC = 273 reads: +427 validated
umi GTTTATTACC = 222 reads: +427 validated
umi TACGAGAATT = 307 reads: +427 validated
umi TATAATCGCC = 358 reads: +427 validated
umi TCTGCCTGGT = 88 reads: +384 -43 non-validated
umi TTACTCACGC = 209 reads: +427 validated
umi TTGCATGTGT = 304 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=568]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
395-432 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 83 umis using 4133 reads
cdr3 = CQQRSNWPLLTF at 368, score = 9 + 9
umis assigned: [19, 24, 32, 44, 51, 62, 67, 69, 77, 89] and 74 others
of which 84 are surviving nonsolos
reads assigned: 27175
start codons at 47, 252, 255, 474
confident = true

TIG 2[bases=557]
0-59 ==> 0-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=4)
59-412 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=1)
438-486 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
486-557 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 20 umis using 383 reads
cdr3 = CARDVEWLQLLTPSYDYW at 401, score = 8 + 7
umis assigned: [75, 179, 216, 235, 270, 276, 279, 305, 349, 352] and 14 others
of which 24 are surviving nonsolos
reads assigned: 5460
start codons at 59, 210, 257, 262, 279, 294, 323, 356, 411, 418
confident = true

REJECT CONTIGS

TIG 1[bases=559]
0-25 ==> 6-31 on |185|IGHV4-34|5'UTR| [len=31] (mis=0)
0-25 ==> 3160-3185 on rc of segment before IGHV7-34-1 exon 2 [len=3185] (mis=0)
4-34 ==> 1353-1383 on rc of segment before IGHV7-81 exon 2 [len=1396] (mis=1)
12-46 ==> 5966-6000 on rc of segment after IGHV4-4 exon 1 [len=6000] (mis=0)
25-396 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=2)
425-488 ==> 0-63 on |59|IGHJ6|J-REGION| [len=63] (mis=1)
488-559 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [56, 137, 472, 733]
of which 4 are surviving nonsolos
reads assigned: 607
start codons at 2, 25, 46, 90, 176, 421, 445
confident = false
full length stopped transcript of length 559
frameshifted full length stopped transcript of length 559
did not find CDR3
now this is a cell
paired!

ACGGCCGTGTATTACTGTGCGAGAGATGTCGAATGGCTACAATTACTCACGCCATCCTACGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> AGCAGCCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTAGCAACTGGCCTCTTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.157 = ACCTTTACATACGCCG-1

using 158 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[2^2, 3^2, 141]
surviving nonsolo ucounts = 1[141]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.158 = ACCTTTACATATGGTC-1

using 467 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 5[2^2, 4, 154, 300]
surviving nonsolo ucounts = 2[154, 300]
ids = [4, 7]

====================================================================================

UMI info for barcode ACCTTTACATATGGTC-1 contig 1 = GCTCTGCTTC...
umi GAGTAGCGCG = 151 reads: +391 validated
umi GGCCGCCTCG = 296 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=558]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=22)
404-442 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=6)
442-558 ==> 0-116 on |307|IGLC3|C-REGION| [len=317] (mis=1)
junction support: 2 umis using 57 reads
cdr3 = CQSYDSILTGWAF at 375, score = 7 + 8
umis assigned: [4, 7]
of which 2 are surviving nonsolos
reads assigned: 444
start codons at 51, 205, 358, 385, 521
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.159 = ACCTTTACATCACAAC-1

using 28 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[5, 22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.161 = ACCTTTACATCACGAT-1

using 244 reads

====================================================================================

graph has 76 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[244]
surviving nonsolo ucounts = 1[244]
ids = [0]

====================================================================================

UMI info for barcode ACCTTTACATCACGAT-1 contig 1 = AGGTTCCAGC...
umi CAGTATGCGC = 224 reads: +418 validated

GOOD CONTIGS

TIG 1[bases=490]
0-58 ==> 87-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
58-408 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=0)
434-476 ==> 19-61 on |58|IGHJ6|J-REGION| [len=61] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CARDGGSGSYYSLDVW at 397, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 219
start codons at 14, 58, 102, 407
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 7.166 = ACCTTTACATCCTTGC-1

using 3589 reads

====================================================================================

graph has 1962 edges initially, 50 edges after simplification

total ucounts = 482
nonsolo ucounts = 211[2^96, 3^44, 4^22, 5^14, 6^10, 7^3, 8^3, 9^2, 10, 12^3, 14, 17, 22, 36, 70, 96, 138, 144, 229, 292, 358, 370, 880]
surviving nonsolo ucounts = 11[17, 22, 70, 96, 138, 144, 229, 292, 358, 370, 880]
ids = [311, 181, 379, 134, 102, 40, 89, 423, 177, 443, ...]

====================================================================================

UMI info for barcode ACCTTTACATCCTTGC-1 contig 1 = GGTCTGCTTC...
umi ACCATGGCGC = 143 reads: +394 validated
umi AGGATTCGCT = 225 reads: +394 validated
umi CCGGATAAGG = 22 reads: -20 +151 -1 +222 non-validated
umi TCAACGGGGT = 881 reads: -358 +3 -1XX +32 invalidated
umi TGGGCTTCAC = 292 reads: +394 validated
umi TTATTGATTG = 374 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=656]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=1)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=0)
407-445 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
445-656 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 5 umis using 163 reads
cdr3 = CQSYDSSLSGSGVF at 375, score = 8 + 8
umis assigned: [40, 89, 181, 365, 423, 443]
of which 6 are surviving nonsolos
reads assigned: 1913
start codons at 51, 205, 208, 259, 358, 385
confident = true

REJECT CONTIGS

TIG 1[bases=746]
0-36 ==> 0-36 on |330|IGLV1-51|5'UTR| [len=36] (mis=0)
36-79 ==> 0-43 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=0)
82-191 ==> 0-109 on segment before IGLV1-51 exon 2 [len=109] (mis=0)
188-498 ==> 43-353 on |331|IGLV1-51|L-REGION+V-REGION| [len=353] (mis=2) [SHIFT!]
497-535 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
535-746 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
umis assigned: [102, 134, 177, 379]
of which 4 are surviving nonsolos
reads assigned: 651
start codons at 36, 122, 299, 350, 474
confident = false
did not find CDR3
now this is a cell
NOT paired!

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BARCODE 7.177 = ACCTTTACATTCTCAT-1

using 47 reads

====================================================================================

graph has 36 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[13, 34]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.07
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk007-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk007-ucc6a7a72ce/files/contig_bam.bam

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12.302 seconds used processing barcodes, peak mem = 0.24
