[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.37 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk005-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk005-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk005.ucc6a7a72ce
n50_n50_rpu = 288

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BARCODE 5.0 = ACATCAGCAAGTCTAC-1

using 24338 reads

====================================================================================

graph has 6643 edges initially, 148 edges after simplification

total ucounts = 582
nonsolo ucounts = 268[2^80, 3^43, 4^35, 5^22, 6^10, 7^8, 8^3, 9^2, 10^5, 11^2, 12^2, 17, 18, 19, 42, 65, 68, 73, 78, 111^2, 114, 139, 156, 161, 169, 181, 190, 193, 195, 204, 205, 219, 227, 242, 257, 259, 261, 262, 266, 273, 291, 317, 345, 404, 479, 523, 570, 580, 611, 665, 680, 709, 720, 726, 753, 769, 784, 809, 829, 830, 848, 888, 900, 913, 928, 1585]
surviving nonsolo ucounts = 53[10, 65, 68, 73, 78, 111^2, 114, 139, 156, 161, 169, 181, 190, 193, 195, 204, 205, 219, 227, 242, 257, 259, 261, 262, 266, 273, 291, 317, 345, 404, 479, 523, 570, 580, 611, 665, 680, 709, 720, 726, 753, 769, 784, 809, 829, 830, 848, 888, 900, 913, 928, 1585]
ids = [268, 527, 522, 498, 283, 41, 460, 387, 52, 193, ...]

====================================================================================

UMI info for barcode ACATCAGCAAGTCTAC-1 contig 1 = GGGATGCTTT...
umi AAGCTCCTCT = 113 reads: +466 validated
umi AATAATTCAG = 145 reads: +466 validated
umi ACAGCAACCG = 263 reads: +463 -3 non-validated
umi ACTGGGACTC = 263 reads: -115X +351 invalidated
umi ATGGGCTGTC = 202 reads: +466 validated
umi CAATCTGGGT = 158 reads: +466 validated
umi CCGCGGTCCG = 259 reads: +466 validated
umi CGGCTGTCGC = 162 reads: +466 validated
umi CTACTTGTTG = 80 reads: +466 validated
umi GCATACAATA = 189 reads: +466 validated
umi GCCCACGTGG = 269 reads: +466 validated
umi TATGAATATA = 199 reads: -1 +1 -1 +2 -2 +120 -1X +338 invalidated
umi TATTAATTTA = 110 reads: +456 -10 non-validated
umi TCGTGCATAT = 271 reads: +466 validated
umi TGATGTCTGG = 320 reads: +466 validated
umi TGGAGTACCT = 244 reads: +466 validated

GOOD CONTIGS

TIG 1[bases=645]
19-396 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=28)
434-485 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=3)
485-645 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 14 umis using 319 reads
cdr3 = CARKGFGMLTFGGLIDKNWFDPW at 385, score = 9 + 7
umis assigned: [41, 52, 68, 105, 165, 193, 243, 270, 283, 350] and 6 others
of which 16 are surviving nonsolos
reads assigned: 3184
start codons at 3, 19, 28, 40, 84, 406, 539
confident = true

REJECT CONTIGS

TIG 1[bases=780]
0-37 ==> 0-37 on |391|IGLV9-49|5'UTR| [len=37] (mis=0)
30-50 ==> 5821-5841 on segment before IGLV2-34 exon 1 [len=6000] (mis=0)
30-50 ==> 5958-5978 on segment before IGLV2-5 exon 1 [len=6137] (mis=0)
37-80 ==> 0-43 on |392|IGLV9-49|L-REGION+V-REGION| [len=369] (mis=0)
83-220 ==> 0-137 on segment before IGLV9-49 exon 2 [len=137] (mis=1)
217-298 ==> 43-124 on |392|IGLV9-49|L-REGION+V-REGION| [len=369] (mis=2) [SHIFT!]
336-533 ==> 172-369 on |392|IGLV9-49|L-REGION+V-REGION| [len=369] (mis=16) [SHIFT!]
531-569 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
569-780 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=1)
umis assigned: [5, 224, 240, 268]
of which 4 are surviving nonsolos
reads assigned: 687
start codons at 37, 200, 480
confident = false
did not find CDR3

TIG 2[bases=305]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=1)
20-83 ==> 5678-5741 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=1)
31-170 ==> 0-139 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=3)
169-305 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [3, 4, 10, 13, 54, 95, 108, 176, 187, 188] and 14 others
of which 24 are surviving nonsolos
reads assigned: 16450
start codons at 31, 37, 93, 106, 211
confident = false
did not find CDR3
now this is a cell
NOT paired!

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BARCODE 5.5 = ACATCAGCACAAGCCC-1

using 13 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.18 = ACATCAGCAGAGCCAA-1

using 46 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 7[2, 3, 4^2, 5, 11, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.27 = ACATCAGCAGGGCATA-1

using 293 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 2[3, 286]
surviving nonsolo ucounts = 1[286]
ids = [4]

====================================================================================

UMI info for barcode ACATCAGCAGGGCATA-1 contig 1 = GAGCTGCTCA...
umi TCTTATATAC = 285 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=554]
0-30 ==> 22-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
30-378 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYGSSPPYTF at 354, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 30, 238, 364, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.30 = ACATCAGCAGTGACAG-1

using 992 reads

====================================================================================

graph has 304 edges initially, 12 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[385, 602]
surviving nonsolo ucounts = 2[385, 602]
ids = [6, 4]

====================================================================================

UMI info for barcode ACATCAGCAGTGACAG-1 contig 1 = GTCAGACTCA...
umi TAGTTCCGCA = 539 reads: -249 +5 -1XX +2 -1XX +15 -1XX +4 -1XX +3 -1XX +25 -1XX +3 -1XX +16 -1XX +3 -3XX +1 -1XX +1 -1XX +2 -11X +2 -1XX +3 -1XX +3 -3XX +5 -1XX +3 -1XX +4 -2XX +1 -1XX +1 -1XX +2 invalidated
umi TTCCTCCTTC = 389 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=17)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CQQYNSYALSF at 350, score = 8 + 7
umis assigned: [4, 6]
of which 2 are surviving nonsolos
reads assigned: 913
start codons at 23, 29, 85, 98, 234, 237, 330, 369, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.33 = ACATCAGCATACTACG-1

using 1033 reads

====================================================================================

graph has 262 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[6, 9, 309, 708]
surviving nonsolo ucounts = 3[9, 309, 708]
ids = [1, 0, 3]

====================================================================================

UMI info for barcode ACATCAGCATACTACG-1 contig 1 = AATCAGTCCC...
umi ACCGATATCG = 311 reads: +388 validated
umi CCAGGCCAAT = 9 reads: -388 non-validated

GOOD CONTIGS

TIG 1[bases=548]
0-24 ==> 3-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
24-375 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CLQYSSSPWTF at 351, score = 9 + 8
umis assigned: [0, 1]
of which 2 are surviving nonsolos
reads assigned: 315
start codons at 24, 30, 99, 235, 454
confident = false

REJECT CONTIGS

TIG 1[bases=350]
1-204 ==> 5797-6000 on rc of segment after IGHD1-1 exon 1 [len=6000] (mis=0)
233-279 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
279-350 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 700
start codons at 16, 118
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.42 = ACATCAGCATGGTCAT-1

using 19 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.51 = ACATCAGGTACCATCA-1

using 1428 reads

====================================================================================

graph has 1934 edges initially, 12 edges after simplification

total ucounts = 606
nonsolo ucounts = 264[2^107, 3^62, 4^41, 5^21, 6^10, 7^9, 8^3, 9^4, 10^3, 12, 13, 15, 164]
surviving nonsolo ucounts = 1[164]
ids = [291]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.69 = ACATCAGGTCTCTTAT-1

using 321 reads

====================================================================================

graph has 162 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 2[2, 310]
surviving nonsolo ucounts = 1[310]
ids = [8]

====================================================================================

UMI info for barcode ACATCAGGTCTCTTAT-1 contig 1 = TGGGGAAAAC...
umi TGCCAGACCT = 292 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=524]
43-395 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=21)
393-431 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
431-524 ==> 0-93 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CSAWDSSLNVWVF at 364, score = 7 + 8
umis assigned: [8]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 43, 182, 372, 389
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.70 = ACATCAGGTCTCTTTA-1

using 54821 reads

====================================================================================

graph has 19037 edges initially, 184 edges after simplification

total ucounts = 2745
nonsolo ucounts = 1417[2^522, 3^277, 4^156, 5^91, 6^60, 7^39, 8^13, 9^8, 10^9, 11^2, 12^5, 13^3, 14, 15^3, 17^2, 18^3, 19^2, 21, 25, 26^2, 28, 38, 40, 41, 45, 47, 48^2, 49, 50, 52, 55^2, 59^2, 60, 69, 70, 72, 74, 79, 80, 84, 85, 93, 95, 100, 101, 102, 104, 105, 120, 121, 122, 128, 130, 131, 134, 141, 148^2, 149^3, 154, 155, 156, 158, 161, 162^2, 164^2, 165, 175, 177, 178, 179, 182^2, 186, 191^2, 194, 195^2, 202, 205^2, 206^2, 207^2, 210^2, 211, 213, 214^2, 216, 217^3, 218^2, 220^2, 221, 222, 223^2, 224^3, 225, 226^3, 227^3, 228, 229^2, 230^2, 233^2, 234^2, 235, 236, 237^3, 238, 239, 240^3, 242, 243^2, 245, 247^2, 248, 249, 251, 254, 256^2, 257, 259, 260^2, 261^2, 262, 263^2, 264, 266, 267^2, 268^2, 271, 272, 275^2, 276, 277^3, 278^3, 279, 281, 284, 285^2, 286^3, 287, 288^2, 289^2, 290^3, 294, 295, 296^3, 297, 298^3, 301, 302, 303^2, 304^2, 305, 308^2, 312, 313, 314, 319, 322, 323^2, 328^2, 331, 335, 337, 343, 344^2, 353, 356, 359, 368, 369, 374, 501, 638, 646, 665]
surviving nonsolo ucounts = 205[2, 41, 47, 52, 55^2, 59^2, 60, 70, 74, 84, 85, 93, 95, 100, 101, 102, 104, 105, 120, 121, 122, 128, 130, 131, 134, 141, 148^2, 149^3, 154, 155, 156, 161, 162^2, 164^2, 165, 175, 177, 178, 179, 182^2, 186, 191^2, 194, 195^2, 202, 205^2, 206^2, 207^2, 210^2, 211, 213, 214^2, 216, 217^3, 218^2, 220^2, 221, 222, 223^2, 224^3, 225, 226^3, 227^3, 228, 229^2, 230^2, 233^2, 234^2, 235, 236, 237^3, 238, 239, 240^3, 242, 243^2, 245, 247^2, 248, 249, 251, 254, 256^2, 257, 259, 260^2, 261^2, 262, 263^2, 264, 266, 267^2, 268^2, 271, 272, 275^2, 276, 277^3, 278^3, 279, 281, 284, 285^2, 286^3, 287, 288^2, 289^2, 290^3, 294, 295, 296^3, 297, 298^3, 301, 302, 303^2, 304^2, 305, 308^2, 312, 313, 314, 319, 322, 323^2, 328^2, 331, 335, 337, 343, 344^2, 353, 356, 359, 368, 369, 374, 501, 638, 646, 665]
ids = [110, 2301, 1669, 1911, 1465, 2493, 185, 2347, 1998, 1889, ...]

====================================================================================

UMI info for barcode ACATCAGGTCTCTTTA-1 contig 1 = GGGGGCTCCA...
umi AAAACCCCGG = 278 reads: +388 validated
umi AAACGTACTT = 200 reads: +388 validated
umi AAAGTCCGAT = 162 reads: +388 validated
umi AACCTCTATA = 231 reads: +388 validated
umi AACGAACTGT = 226 reads: +388 validated
umi AAGACAGGGT = 318 reads: +388 validated
umi AAGGTATCGT = 261 reads: +388 validated
umi AAGTTAGGCT = 255 reads: +388 validated
umi AATTTGTTTG = 230 reads: +388 validated
umi ACATTTAAGG = 278 reads: +352 -1XX +2 -1X +32 invalidated
umi ACCCAGTGGC = 235 reads: +388 validated
umi ACGTGGCTGC = 242 reads: +388 validated
umi ACTGGTCAAA = 227 reads: +388 validated
umi ACTTGCGCAG = 275 reads: +388 validated
umi AGGACGCAGC = 264 reads: +388 validated
umi AGTAAAAATG = 147 reads: +388 validated
umi ATCCACGGTA = 1 reads: -24X +1 -4X +2 -9X +1 -3X +32 -312 invalidated
umi ATGCCTTCCG = 261 reads: +388 validated
umi ATTCATTCCC = 239 reads: +388 validated
umi CAACATGCTT = 302 reads: +388 validated
umi CAAGCCTGCA = 205 reads: +388 validated
umi CACAATTCTG = 221 reads: +388 validated
umi CATATTATTG = 298 reads: +388 validated
umi CATTCTCTTG = 226 reads: +388 validated
umi CCAGCATTGA = 123 reads: +388 validated
umi CCTATTAGCG = 232 reads: +388 validated
umi CCTGCTTCTC = 242 reads: +388 validated
umi CGAAGGTTAT = 176 reads: +388 validated
umi CGGTTCTACA = 310 reads: +388 validated
umi CGTCCGTGTC = 223 reads: +388 validated
umi CTCGATCTGC = 225 reads: +388 validated
umi CTCGTTGCAC = 211 reads: +388 validated
umi CTTAACAGCT = 138 reads: -15X +2 -7XX +1 -4XX +2 -9XX +1 -3XX +344 invalidated
umi CTTCATCCAC = 203 reads: +388 validated
umi GAAGTAAGAC = 197 reads: +388 validated
umi GCTCACCGGC = 269 reads: +388 validated
umi GGAAAAGTAC = 218 reads: +388 validated
umi GGACGTGGGC = 233 reads: +388 validated
umi GTAACGACTC = 287 reads: +388 validated
umi GTATGTCTTC = 204 reads: +388 validated
umi GTGTTCGCCT = 242 reads: +388 validated
umi GTTCACGGGC = 217 reads: +388 validated
umi GTTCTCCTGG = 234 reads: +388 validated
umi TATACGGCGC = 276 reads: +388 validated
umi TATTATTATT = 506 reads: +388 validated
umi TCGAACGTCC = 206 reads: +388 validated
umi TCTGCCACAG = 167 reads: +388 validated
umi TGGTTACTGC = 210 reads: +388 validated
umi TGTGCTCGAT = 177 reads: +388 validated
umi TGTTTTGGGG = 225 reads: +388 validated
umi TTAAAATTGG = 290 reads: +388 validated
umi TTGCAGCCGA = 243 reads: +388 validated
umi TTGGGAATCA = 277 reads: +388 validated
umi TTGTCATAGG = 344 reads: +388 validated
umi TTTACGGACA = 136 reads: +388 validated
umi TTTACGGATA = 305 reads: +388 validated
umi TTTCCCCACT = 241 reads: +388 validated
umi TTTCTGTCCG = 215 reads: +388 validated
umi TTTGTCGGAG = 292 reads: +388 validated

UMI info for barcode ACATCAGGTCTCTTTA-1 contig 2 = GAGCTCTGGG...
umi AAAATGCTCC = 345 reads: +399 -2 +5 non-validated
umi AAACAACCGA = 221 reads: +406 validated
umi ACAGGGTTAC = 59 reads: +369 -1 +2 -34 non-validated
umi ACCCAACAAA = 644 reads: -132X +274 invalidated
umi ATCTATGCCA = 364 reads: +406 validated
umi ATGTGTCACA = 278 reads: +406 validated
umi CAAAGCTTAT = 149 reads: +406 validated
umi CAACTGGCTT = 83 reads: +396 -10 non-validated
umi CACGGCATCC = 231 reads: +406 validated
umi CACTAGCACA = 255 reads: +397 -9 non-validated
umi CAGCTATCCC = 241 reads: +395 -11 non-validated
umi CATGAACCGA = 103 reads: +360 -1 +15 -1 +4 -1 +7 -17 non-validated
umi CATTCGACGG = 297 reads: +406 validated
umi CCCACGGCCA = 267 reads: +165 -3XX +1 -9XX +1 -1XX +1 -33XX +1 -3XX +1 -5XX +1 -1XX +1 -13XX +166 invalidated
umi CCCGCACTCG = 265 reads: +383 -23 non-validated
umi CCGCTAAGTT = 141 reads: +346 -19 +32 -1 +8 non-validated
umi CCGCTGTACC = 305 reads: +406 validated
umi CCGTGACCCG = 228 reads: +406 validated
umi CCTCGATCCG = 164 reads: +375 -1 +8 -22 non-validated
umi CGCCACAGGC = 262 reads: +406 validated
umi CGTAATCCGT = 98 reads: +340 -1 +15 -50 non-validated
umi CTATTCCTGG = 223 reads: +399 -1 +1 -1 +4 non-validated
umi CTTCGGTTCT = 153 reads: +403 -3 non-validated
umi CTTTTCGTAT = 53 reads: +281 -12 +2 -1 +9 -1 +1 -1 +98 non-validated
umi GCATGCCGCC = 75 reads: +395 -11 non-validated
umi GCGCGTACTG = 242 reads: +397 -9 non-validated
umi GCTCGGGAGG = 275 reads: +406 validated
umi GGCCAAAACA = 46 reads: +346 -9 +15 -2 +5 -2 +11 -2 +14 non-validated
umi GTCATCTCTG = 212 reads: +357 -49 non-validated
umi GTCCCATTTT = 265 reads: +375 -2 +2 -27 non-validated
umi GTTTCTAGCA = 303 reads: +8 -1XX +397 invalidated
umi TAAACATCCT = 188 reads: +375 -1 +5 -1 +4 -20 non-validated
umi TAAACGGGCT = 106 reads: +334 -1XX +71 invalidated
umi TAACACTTAC = 69 reads: +313 -93 non-validated
umi TATCGAGCCT = 60 reads: +383 -23 non-validated
umi TCACTTTACG = 220 reads: +406 validated
umi TCGGTTTCCA = 144 reads: +406 validated
umi TGAATACGCA = 41 reads: +296 -1 +27 -82 non-validated
umi TGCCCCCTGA = 244 reads: +400 -2 +4 non-validated
umi TGCGCCCAAT = 166 reads: +406 validated
umi TGCGCTAGGG = 60 reads: +355 -1 +40 -10 non-validated
umi TGTGTAATCA = 147 reads: +313 -1 +63 -29 non-validated
umi TTAATTCATC = 87 reads: +367 -39 non-validated
umi TTACACTCTC = 287 reads: +386 -20 non-validated
umi TTACCTTGAC = 131 reads: +332 -1 +73 non-validated
umi TTACTGTGTT = 43 reads: -372 +34 non-validated
umi TTAGTTATTT = 149 reads: +406 validated
umi TTATGCCGGG = 103 reads: +347 -59 non-validated
umi TTATTTCGGG = 103 reads: +348 -1 +1 -56 non-validated
umi TTCGGATAGA = 193 reads: +369 -31 +6 non-validated
umi TTGACATCTA = 226 reads: +26 -1XX +1 -4XX +1 -5XX +368 invalidated
umi TTGGCTCACA = 182 reads: +362 -1X +16 -27 invalidated
umi TTTGTGGGCT = 148 reads: +369 -37 non-validated
umi TTTTTCATAT = 161 reads: +406 validated

UMI info for barcode ACATCAGGTCTCTTTA-1 contig 3 = GTGGGCTCAG...
umi ACAAAGTACA = 219 reads: +382 validated
umi ATACCTTCGT = 235 reads: +382 validated
umi GAACACTGGG = 235 reads: +382 validated
umi GGCTGGCAGA = 284 reads: +382 validated
umi GGTTCACCGA = 314 reads: +382 validated
umi TGAGCCCACA = 130 reads: +382 validated
umi TGCGTCCACT = 216 reads: +382 validated
umi TTATATTCGT = 213 reads: +382 validated

UMI info for barcode ACATCAGGTCTCTTTA-1 contig 4 = GGAGTCTCCC...
umi ACCACGTCTT = 234 reads: -347 +1 -4XX +1 -1XX +1 -1XX +2 -3XX +1 -1XX +2 -2XX +1 -2XX +1 -1XX +3 -2XX +35 invalidated
umi AGTCCGGCGG = 256 reads: +390 -1 +21 non-validated
umi ATCGACTTTC = 249 reads: +412 validated
umi CTACTTCGTC = 156 reads: -366 +2 -2X +1 -1XX +3 -2XX +9 -1XX +2 -1XX +3 -2XX +17 invalidated
umi CTTAATCGTT = 275 reads: -345X +1 -1XX +1 -4XX +1 -1XX +1 -1XX +2 -3XX +1 -1XX +2 -2XX +1 -2XX +1 -1XX +3 -2XX +35 invalidated
umi GTAGGAGCGC = 506 reads: -385X +1 -2XX +1 -1XX +1 -1XX +1 -3XX +1 -2XX +1 -1XX +1 -1XX +2 -2XX +1 -1XX +3 invalidated
umi TAATTATTGT = 38 reads: -383X +2 -9X +2 -2X +1 -2X +2 -2X +7 invalidated
umi TTCGTATCAT = 231 reads: +412 validated

GOOD CONTIGS

TIG 1[bases=645]
46-398 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=21)
396-434 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=1)
434-645 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 58 umis using 2137 reads
cdr3 = CSAWDSSLNVWVF at 367, score = 7 + 8
umis assigned: [4, 23, 36, 62, 66, 90, 104, 109, 158, 208] and 49 others
of which 58 are surviving nonsolos
reads assigned: 13698
start codons at 46, 185, 375, 392
confident = true

TIG 2[bases=557]
0-80 ==> 0-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=1)
80-431 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=23)
436-486 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
486-557 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 21 umis using 338 reads
cdr3 = CVKEEDAFDIW at 422, score = 9 + 8
umis assigned: [11, 14, 185, 222, 518, 575, 647, 659, 713, 723] and 44 others
of which 54 are surviving nonsolos
reads assigned: 9897
start codons at 80, 231, 236, 297, 315, 383, 438, 467
confident = true

TIG 3[bases=628]
0-35 ==> 0-35 on |350|IGLV3-10|5'UTR| [len=35] (mis=0)
35-382 ==> 0-347 on |351|IGLV3-10|L-REGION+V-REGION| [len=347] (mis=1)
379-417 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
417-628 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 8 umis using 297 reads
cdr3 = CYSTDSSGNHWVF at 350, score = 7 + 8
umis assigned: [161, 463, 1475, 1678, 1720, 2308, 2351, 2509]
of which 8 are surviving nonsolos
reads assigned: 1794
start codons at 35, 96, 165, 183, 234, 296, 333
confident = true

TIG 4[bases=542]
0-59 ==> 0-59 on |202|IGHV5-51|5'UTR| [len=59] (mis=1)
59-412 ==> 0-353 on |203|IGHV5-51|L-REGION+V-REGION| [len=353] (mis=3)
423-471 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
471-542 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 3 umis using 34 reads
cdr3 = CARLGVHKGVDYW at 401, score = 8 + 7
umis assigned: [216, 436, 508, 1246, 1394, 1744, 1911, 2571]
of which 8 are surviving nonsolos
reads assigned: 1915
start codons at 59, 233, 257, 392
confident = true

REJECT CONTIGS

TIG 1[bases=571]
0-36 ==> 0-36 on |272|IGKV2D-28|5'UTR| [len=36] (mis=4)
6-81 ==> 5533-5608 on segment before IGKV2OR2-1 exon 1 [len=6000] (mis=2)
36-396 ==> 0-360 on |273|IGKV2D-28|L-REGION+V-REGION| [len=360] (mis=4)
397-435 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
435-571 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CWDLLLHARSTNSSITF at 356, score = 4 + 8
umis assigned: [42, 72, 111, 121, 278, 352, 354, 409, 427, 477] and 62 others
of which 72 are surviving nonsolos
reads assigned: 19057
start codons at 36, 69, 105, 193, 355, 375, 477
confident = false
not full
frameshifted full length transcript of length 571
VJ delta = 30
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.74 = ACATCAGGTGCGAAAC-1

using 319 reads

====================================================================================

graph has 129 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 315]
surviving nonsolo ucounts = 1[315]
ids = [2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=528]
1-347 ==> 7-353 on |209|IGHV7-4-1|L-REGION+V-REGION| [len=353] (mis=12)
407-457 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
457-528 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 68, 150, 192, 229, 400, 409, 438
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.79 = ACATCAGGTGTCAATC-1

using 233 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 227]
surviving nonsolo ucounts = 1[227]
ids = [0]

====================================================================================

UMI info for barcode ACATCAGGTGTCAATC-1 contig 1 = GTCAGACCCT...
umi AATACCGATC = 228 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=547]
0-29 ==> 24-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
29-374 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
383-411 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQYYSYPRTF at 350, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 224
start codons at 23, 29, 85, 98, 234, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.92 = ACATCAGGTTGGTAAA-1

using 232 reads

====================================================================================

graph has 228 edges initially, 14 edges after simplification

total ucounts = 45
nonsolo ucounts = 35[2^5, 3^5, 4^7, 5^3, 6^5, 7^2, 8, 10, 12, 13, 14, 16, 17, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.94 = ACATCAGGTTTGTTTC-1

using 86 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2^2, 77]
surviving nonsolo ucounts = 1[77]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.97 = ACATCAGTCACAAACC-1

using 3217 reads

====================================================================================

graph has 4117 edges initially, 26 edges after simplification

total ucounts = 1358
nonsolo ucounts = 591[2^251, 3^139, 4^84, 5^48, 6^28, 7^13, 8^14, 9^4, 10^2, 13, 14^3, 16, 18, 200, 238]
surviving nonsolo ucounts = 2[200, 238]
ids = [351, 387]

====================================================================================

UMI info for barcode ACATCAGTCACAAACC-1 contig 1 = AGCTTCAGCT...
umi ATTATAGACT = 201 reads: +388 validated
umi CAACCCTTCT = 238 reads: +388 validated
umi TTAATCGGCA = 3 reads: -197 +10 -1X +1 -2X +1 -1X +1 -3X +1 -1X +10 -1 +20 -2 +1 -135 invalidated

GOOD CONTIGS

TIG 1[bases=566]
0-46 ==> 10-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
46-397 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=1)
396-434 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
434-566 ==> 0-132 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 2 umis using 66 reads
cdr3 = CAAWDDSLSGVVF at 367, score = 7 + 8
umis assigned: [351, 387, 1217]
of which 2 are surviving nonsolos
reads assigned: 435
start codons at 46, 200, 350, 375, 380
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.104 = ACATCAGTCAGCTCTC-1

using 72 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 20
nonsolo ucounts = 13[2^3, 3^2, 4^2, 5, 6^2, 9^2, 10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.109 = ACATCAGTCATACGGT-1

using 25602 reads

====================================================================================

graph has 8908 edges initially, 70 edges after simplification

total ucounts = 1033
nonsolo ucounts = 528[2^158, 3^98, 4^65, 5^38, 6^35, 7^22, 8^13, 9^4, 10^5, 11^4, 12^2, 13^2, 14, 18, 28^2, 30, 48, 58, 64, 83, 96, 100, 117^2, 130, 137, 151, 152, 153, 159, 165, 166, 168, 182, 194, 197, 198, 199, 203, 206^2, 208, 218, 219, 221, 225, 227, 230^2, 234, 235, 236, 238, 240, 243^2, 252, 255, 261, 263, 264, 268, 276, 280, 289, 291, 293, 306, 313, 317, 327, 328, 343, 345, 346, 360, 367, 369, 385, 386, 392, 395, 404, 417^2, 443, 505, 564, 780, 811, 1086, 1160, 1317]
surviving nonsolo ucounts = 75[48, 64, 83, 96, 100, 117^2, 130, 137, 151, 153, 159, 165, 166, 168, 182, 194, 197, 198, 199, 203, 206^2, 208, 218, 219, 221, 225, 227, 230^2, 234, 235, 236, 238, 240, 243^2, 252, 255, 261, 263, 264, 268, 276, 280, 289, 291, 293, 306, 313, 317, 327, 328, 343, 345, 346, 360, 367, 369, 385, 386, 392, 395, 404, 417^2, 443, 505, 564, 780, 811, 1086, 1160, 1317]
ids = [389, 142, 460, 183, 697, 182, 433, 1002, 414, 500, ...]

====================================================================================

UMI info for barcode ACATCAGTCATACGGT-1 contig 1 = AGTGCTTTCT...
umi AAAGGGTCGT = 207 reads: +430 validated
umi AATAAATATC = 203 reads: +430 validated
umi ACCTGTGCGG = 239 reads: +430 validated
umi ACTTTAATTC = 64 reads: +406 -24 non-validated
umi AGCTTTCCTC = 416 reads: +430 validated
umi AGTTGTGCAA = 133 reads: +430 validated
umi AGTTTATCTA = 87 reads: -4 +426 non-validated
umi ATAAACTAGC = 205 reads: +430 validated
umi ATAAATTTGC = 370 reads: +430 validated
umi ATTTTTGGAT = 143 reads: +430 validated
umi ATTTTTTTGG = 402 reads: +430 validated
umi CAATTGCCCT = 371 reads: +430 validated
umi CAATTTGCAG = 198 reads: +430 validated
umi CAGATTGTAG = 412 reads: +165 -1XX +264 invalidated
umi CAGCTGGCTT = 383 reads: +430 validated
umi CATATCCGTT = 257 reads: +430 validated
umi CCGCGGCTCT = 47 reads: -28 +402 non-validated
umi CGAGCAATTA = 116 reads: +430 validated
umi CGGATAAGTG = 84 reads: +339 -1 +5 -3 +82 non-validated
umi CGTCCTCAAG = 201 reads: +118 -1X +1 -2X +306 -1 +1 invalidated
umi CTAACCAAGG = 270 reads: +430 validated
umi CTAGGGACGC = 345 reads: +430 validated
umi CTCTCGCTCT = 219 reads: +430 validated
umi GACCCCGTCA = 290 reads: +430 validated
umi GCAGATTAGG = 226 reads: +430 validated
umi GGAACGCCAC = 166 reads: +430 validated
umi GGCATGGGCC = 278 reads: +430 validated
umi GTAAACTTTC = 89 reads: +430 validated
umi GTTCATTATT = 283 reads: +430 validated
umi TAACCTATAC = 154 reads: +430 validated
umi TACTCTCTCC = 169 reads: +430 validated
umi TATAAAGTAG = 328 reads: +430 validated
umi TCGGCTCACA = 148 reads: +430 validated
umi TCTCAGATCC = 309 reads: +363 -1XX +66 invalidated
umi TGGCACGGGT = 296 reads: +426 -4 non-validated
umi TTGGAATAGA = 121 reads: +430 validated

UMI info for barcode ACATCAGTCATACGGT-1 contig 2 = GAGCTACAAC...
umi AAAGGGAGGT = 345 reads: +400 validated
umi AACCCCCATT = 413 reads: -343X +1 -3XX +1 -1XX +1 -14XX +36 invalidated
umi AAGCGCTATC = 325 reads: +400 validated
umi ACCGCCGGGA = 400 reads: +400 validated
umi ACTATTCCGC = 262 reads: +400 validated
umi ATTCACTCCA = 2 reads: -337 +1 -5X +1 -3X +1 -1X +1 -14X +36 invalidated
umi CAGCCACAGC = 337 reads: +400 validated
umi CAGCTCAGCA = 386 reads: -330X +3 -1X +1 -2XX +1 -5XX +1 -3XX +1 -1XX +1 -14XX +36 invalidated
umi CAGGACCTTC = 345 reads: +400 validated
umi CAGTTGCTCC = 243 reads: +400 validated
umi CATTTGGCAC = 763 reads: -332 +1 -1XX +1 -2XX +1 -5XX +1 -3XX +1 -1XX +1 -14XX +36 invalidated
umi CCAGTAGCCC = 205 reads: +400 validated
umi CGCTGGTTCG = 249 reads: +97 -1XX +302 invalidated
umi CGCTTGATAG = 257 reads: +400 validated
umi CTATATGGGA = 151 reads: +400 validated
umi CTATGCGGGC = 220 reads: -330X +3 -1XX +1 -2XX +1 -5XX +1 -3XX +1 -1XX +1 -14XX +36 invalidated
umi CTCGTCCGAG = 336 reads: +400 validated
umi GAGCTATATC = 242 reads: +400 validated
umi TAACCCATTC = 331 reads: +400 validated
umi TAGGTAGGGC = 224 reads: +400 validated
umi TAGTCCATCC = 38 reads: -330X +1 -3X +1 -2XX +1 -1XX +1 -9XX +1 -14XX +36 invalidated
umi TATGAGCGTC = 244 reads: +400 validated
umi TCTACACCAC = 200 reads: +400 validated
umi TCTAGTACTC = 291 reads: +400 validated
umi TGAAATCGGG = 192 reads: -364X +36 invalidated
umi TGCTCGATTC = 337 reads: -330X +3 -1XX +1 -2XX +1 -5XX +1 -3XX +1 -1XX +1 -14XX +36 invalidated
umi TTAGTCCGCA = 225 reads: +400 validated
umi TTCTATGCGC = 232 reads: +400 validated
umi TTGGCTACCA = 617 reads: -330X +3 -1XX +1 -2XX +1 -5XX +1 -3XX +1 -1XX +1 -14XX +36 invalidated

GOOD CONTIGS

TIG 1[bases=518]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=1)
399-447 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
447-518 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 33 umis using 678 reads
cdr3 = CARAKRSYGFDYW at 377, score = 9 + 7
umis assigned: [12, 52, 108, 142, 169, 182, 183, 187, 188, 256] and 26 others
of which 36 are surviving nonsolos
reads assigned: 8069
start codons at 17, 38, 82, 168, 399
confident = true

TIG 2[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=0)
392-430 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 20 umis using 678 reads
cdr3 = CQQYYSTPRTF at 369, score = 9 + 8
umis assigned: [11, 24, 41, 103, 125, 236, 302, 308, 310, 317] and 19 others
of which 29 are surviving nonsolos
reads assigned: 8249
start codons at 30, 99, 352, 472
confident = true

REJECT CONTIGS

TIG 1[bases=333]
36-61 ==> 4932-4957 on rc of segment before IGLL1 exon 1 [len=6445] (mis=0)
84-122 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
120-168 ==> 6397-6445 on rc of segment before IGLL1 exon 1 [len=6445] (mis=4)
122-333 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
umis assigned: [105, 121, 303, 430, 653, 659, 677]
of which 7 are surviving nonsolos
reads assigned: 3817
start codons at 86, 254
confident = false
did not find CDR3
now this is a cell
paired!

GTGACCGCCGCGGACACGGCTGTGTATTACTGTGCGAGAGCGAAGAGGAGCTATGGTTTCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCAATATTATAGTACTCCTCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.117 = ACATCAGTCCATGCTC-1

using 150 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[5, 140]
surviving nonsolo ucounts = 1[140]
ids = [0]

====================================================================================

UMI info for barcode ACATCAGTCCATGCTC-1 contig 1 = GGGGGGGGTC...
umi AATCTAAGAC = 130 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=448]
42-393 ==> 0-351 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
392-430 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
junction support: 1 umis using 16 reads
cdr3 = CSSYTSISTVLF at 366, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 127
start codons at 42, 199, 243, 250, 253
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.124 = ACATCAGTCCTGCAGG-1

using 2323 reads

====================================================================================

graph has 1422 edges initially, 36 edges after simplification

total ucounts = 290
nonsolo ucounts = 97[2^33, 3^23, 4^10, 5^7, 6^3, 7^3, 8^4, 9, 11^4, 13, 25, 124, 200, 214, 250, 270, 312, 388]
surviving nonsolo ucounts = 8[25, 124, 200, 214, 250, 270, 312, 388]
ids = [79, 21, 180, 284, 200, 259, 205, 95]

====================================================================================

UMI info for barcode ACATCAGTCCTGCAGG-1 contig 1 = TGGGGGACTG...
umi CGAAGTGCTA = 390 reads: +394 validated
umi TGTGCCCATC = 274 reads: +394 validated

UMI info for barcode ACATCAGTCCTGCAGG-1 contig 2 = TGGGGGATCA...
umi CATTCACGCA = 25 reads: +273 -1 +29 -2 +2 -1 +89 non-validated
umi GTCACCCGCC = 203 reads: +397 validated
umi TACCCGTTCT = 262 reads: +205 -1XX +191 invalidated
umi TATCCAAGCG = 331 reads: +205 -1XX +191 invalidated
umi TTTCTACTAT = 215 reads: +397 validated

GOOD CONTIGS

TIG 1[bases=569]
39-399 ==> 0-360 on |267|IGKV2-30|L-REGION+V-REGION| [len=360] (mis=4)
395-433 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=3)
433-569 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 80 reads
cdr3 = CMQGTHSVTF at 375, score = 9 + 8
umis assigned: [95, 259]
of which 2 are surviving nonsolos
reads assigned: 653
start codons at 39, 72, 100, 108, 196, 358, 378, 475
confident = true

TIG 2[bases=568]
35-395 ==> 0-360 on |265|IGKV2-28|L-REGION+V-REGION| [len=360] (mis=0)
393-432 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 163 reads
cdr3 = CMQALQTPYTF at 371, score = 9 + 8
umis assigned: [79, 180, 200, 205, 284]
of which 5 are surviving nonsolos
reads assigned: 989
start codons at 35, 68, 104, 192, 354, 374, 474
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.128 = ACATCAGTCGACCAGC-1

using 63 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 9[3, 4^4, 5, 9, 12, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.136 = ACATCAGTCTACTATC-1

using 51 reads

====================================================================================

graph has 80 edges initially, 6 edges after simplification

total ucounts = 25
nonsolo ucounts = 13[2^5, 3^6, 5, 6]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.140 = ACATCAGTCTCATTCA-1

using 306 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[3^2, 4, 291]
surviving nonsolo ucounts = 1[291]
ids = [1]

====================================================================================

UMI info for barcode ACATCAGTCTCATTCA-1 contig 1 = TGGGGAGGAA...
umi ATGAATTCTT = 287 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=464]
32-383 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
382-420 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
420-464 ==> 0-44 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 54 reads
cdr3 = CLQYSSSPWTF at 359, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 284
start codons at 32, 38, 107, 243
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.141 = ACATCAGTCTCGTATT-1

using 721 reads

====================================================================================

graph has 227 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[319, 396]
surviving nonsolo ucounts = 2[319, 396]
ids = [2, 4]

====================================================================================

UMI info for barcode ACATCAGTCTCGTATT-1 contig 1 = GGAGCCCAGC...
umi AGCTCGCTTC = 311 reads: +445 validated
umi ATCTCGCTTC = 385 reads: +445 validated

GOOD CONTIGS

TIG 1[bases=529]
0-67 ==> 0-67 on |127|IGHV3-33|5'UTR| [len=67] (mis=1)
67-420 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=0)
426-454 ==> 0-28 on |19|IGHD3-16|D-REGION| [len=37] (mis=1)
462-512 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=1)
junction support: 2 umis using 27 reads
cdr3 = CARDALYYDYIWGSYFLRDAFDIW at 409, score = 9 + 8
umis assigned: [2, 4]
of which 2 are surviving nonsolos
reads assigned: 687
start codons at 67, 218, 223, 281, 284, 370, 419, 431, 464, 493
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.153 = ACATCAGTCTTTACGT-1

using 58089 reads

====================================================================================

graph has 23258 edges initially, 230 edges after simplification

total ucounts = 4276
nonsolo ucounts = 2150[2^823, 3^411, 4^274, 5^166, 6^106, 7^75, 8^34, 9^16, 10^12, 11^7, 12^9, 13^7, 14^3, 15^3, 17^2, 18^2, 20, 23, 25, 31, 33, 38, 39, 43, 45, 48, 50, 51, 55, 57, 60, 61, 64, 68^2, 75, 77, 78, 82, 85, 91^2, 95, 98, 99, 102, 103, 109, 113, 118, 122^2, 124, 128, 132, 135, 138, 141, 145, 149, 150, 152, 153, 156, 157, 165^2, 176, 179, 180^2, 181, 182, 183^2, 185^2, 189, 191, 199^2, 200, 202^2, 203^2, 205^3, 206^2, 207^2, 208, 216, 219, 220, 222^2, 225, 226^2, 230^2, 232, 234^2, 235, 237^2, 238^2, 239^4, 240, 241, 242, 244, 245^2, 246, 247, 250^2, 251, 252, 254, 255, 257, 261^2, 263^2, 264, 265, 266, 268, 269^2, 270^2, 272, 273^2, 275^3, 276^3, 279, 280, 282^2, 283, 286, 287, 288^2, 294, 296, 300^2, 302^3, 303^4, 307, 309, 313, 314, 316^2, 317^2, 318, 320^2, 321, 322, 327, 328^2, 329^2, 331, 332, 338, 343, 349, 357, 362^2, 369, 371, 374, 390, 392, 407, 412, 514, 527, 589, 616, 621, 636, 705, 779, 782, 848, 872]
surviving nonsolo ucounts = 187[2^4, 15, 60, 68^2, 75, 77, 78, 82, 85, 91^2, 95, 98, 102, 103, 109, 113, 118, 122^2, 124, 128, 132, 135, 138, 141, 145, 149, 150, 152, 153, 156, 157, 165^2, 176, 179, 180^2, 181, 182, 183^2, 185^2, 189, 191, 199, 200, 202^2, 203^2, 205^3, 206^2, 207^2, 208, 216, 219, 220, 222^2, 225, 226^2, 230^2, 232, 234^2, 235, 237^2, 238^2, 239^4, 240, 241, 242, 244, 245^2, 246, 247, 250^2, 251, 252, 254, 255, 257, 261^2, 263^2, 264, 265, 266, 268, 269^2, 270^2, 272, 273^2, 275^3, 276^3, 279, 280, 282^2, 283, 286, 287, 288^2, 294, 296, 300^2, 302^3, 303^4, 307, 309, 313, 314, 316^2, 317^2, 318, 320^2, 321, 322, 327, 328^2, 329^2, 331, 332, 338, 343, 349, 357, 362^2, 369, 371, 374, 390, 392, 407, 412, 514, 527, 589, 616, 621, 636, 705, 779, 782, 848, 872]
ids = [215, 2692, 3057, 3392, 3964, 1507, 1344, 2995, 2318, 1873, ...]

====================================================================================

UMI info for barcode ACATCAGTCTTTACGT-1 contig 1 = TGGGGATCAC...
umi AAATCACCGG = 182 reads: +409 validated
umi AACTTACCGC = 122 reads: +377 -1 +14 -17 non-validated
umi ACATATTCCT = 364 reads: +409 validated
umi ACCACCCTAT = 144 reads: +291 -1XX +117 invalidated
umi ACGGTCTTCG = 89 reads: +264 -1X +26 -1XX +117 invalidated
umi ACGTATGGCT = 327 reads: +409 validated
umi ACTCGTTACA = 207 reads: +409 validated
umi ACTTTCGGGA = 278 reads: +409 validated
umi ACTTTTTGGT = 147 reads: -371X +1 -2XX +7 -1XX +1 -1XX +2 -1XX +3 -1XX +18 invalidated
umi AGATCGGGTT = 153 reads: +401 -8 non-validated
umi AGTCTCGGCT = 325 reads: +409 validated
umi AGTTAGTTAG = 277 reads: +291 -1XX +117 invalidated
umi AGTTTGCGTC = 229 reads: +409 validated
umi ATAATAGGGG = 122 reads: +409 validated
umi ATAGGTGATA = 297 reads: -345X +1 -8XX +1 -5XX +1 -10XX +20 -1XX +17 invalidated
umi ATCATTTCCG = 184 reads: +394 -15 non-validated
umi ATGCAACCCA = 290 reads: +409 validated
umi ATTGGGCCTG = 254 reads: +291 -1XX +117 invalidated
umi ATTGTTCCTA = 129 reads: +291 -1XX +88 -17 +12 invalidated
umi CAATGTATTG = 342 reads: +409 validated
umi CAATTATCGT = 272 reads: +409 validated
umi CACATACCAG = 253 reads: +409 validated
umi CAGCCACCGT = 197 reads: +409 validated
umi CAGGTATCTT = 474 reads: -398X +2 -1XX +1 -6XX +1 invalidated
umi CATCAGCCTT = 272 reads: +409 validated
umi CATGCTGAGT = 180 reads: +409 validated
umi CATTATAATG = 67 reads: +306 -5 +23 -1 +74 non-validated
umi CATTCTCCGT = 268 reads: +291 -1XX +117 invalidated
umi CCAAGCCTAC = 200 reads: +409 validated
umi CCACGGTCGT = 324 reads: +291 -1XX +117 invalidated
umi CCATTCGGTG = 328 reads: +408 -1 non-validated
umi CCCTCCAGGC = 244 reads: +291 -1XX +117 invalidated
umi CCGGATATTC = 91 reads: +389 -2 +9 -9 non-validated
umi CCGGATCGGC = 195 reads: +409 validated
umi CCTGAAATTA = 308 reads: +291 -1XX +117 invalidated
umi CCTGCAATTT = 286 reads: +291 -1XX +94 -23 invalidated
umi CGGACACTAC = 228 reads: +409 validated
umi CGGCACTATC = 245 reads: +291 -1XX +117 invalidated
umi CGGTACGGCT = 301 reads: +409 validated
umi CGGTCCCTTA = 101 reads: +333 -1XX +60 -15 invalidated
umi CGTATAATCC = 125 reads: +291 -1XX +117 invalidated
umi CGTTAAACTG = 382 reads: -371X +1 -2XX +7 -1XX +1 -1XX +2 -1XX +3 -1XX +18 invalidated
umi CTACTCCTCT = 67 reads: +328 -1X +65 -1 +1 -1 +3 -1 +8 invalidated
umi CTATTGTCTC = 270 reads: +409 validated
umi CTATTTTACC = 282 reads: +409 validated
umi CTCACTACGC = 325 reads: +291 -1XX +117 invalidated
umi CTCCGCTCCC = 324 reads: +391 -12 +6 non-validated
umi CTCTAGTCTG = 146 reads: +409 validated
umi CTGCCCTCTA = 371 reads: +291 -1XX +117 invalidated
umi CTGGTACCCT = 268 reads: +409 validated
umi CTGTGGCTTT = 245 reads: +409 validated
umi CTTCGCTCAT = 202 reads: +409 validated
umi GAAAAGGCAC = 256 reads: +409 validated
umi GAACTCATCG = 148 reads: +394 -15 non-validated
umi GAATCGTCAT = 189 reads: +291 -1XX +117 invalidated
umi GAGAATAGGG = 270 reads: +409 validated
umi GATCAAAGGG = 239 reads: +409 validated
umi GCAATCTACC = 244 reads: +406 -1 +2 non-validated
umi GCCTAGCAGG = 142 reads: +393 -16 non-validated
umi GCCTTTCGCT = 321 reads: +409 validated
umi GCGATGCGCT = 202 reads: +403 -1 +5 non-validated
umi GCGATTATCT = 223 reads: +393 -3 +8 -3 +1 -1 non-validated
umi GGACCCAGCA = 185 reads: +409 validated
umi GGGATACGGG = 97 reads: +50 -1XX +240 -1XX +52 -1 +64 invalidated
umi GTAGGTACTA = 285 reads: +409 validated
umi GTATAGGTCG = 244 reads: +409 validated
umi GTCGATATTC = 208 reads: +342 -1XX +66 invalidated
umi GTTAATATGG = 315 reads: +291 -1XX +117 invalidated
umi GTTTAGTAGC = 91 reads: +291 -1XX +108 -9 invalidated
umi TAAACAAGCC = 277 reads: +291 -1XX +117 invalidated
umi TAAGAGCTAG = 70 reads: +4 -1 +2 -1 +4 -1 +2 -11 +187 -1XX +155 -1 +4 -3 +32 invalidated
umi TAGGTCTACC = 309 reads: +291 -1XX +117 invalidated
umi TATACCGGTC = 354 reads: +88 -1XX +320 invalidated
umi TCAATGGCGA = 209 reads: +390 -19 non-validated
umi TCAATGGGAA = 218 reads: +409 validated
umi TCACATAATG = 80 reads: +370 -1 +7 -2 +29 non-validated
umi TCAGTTGCGC = 192 reads: +409 validated
umi TCATAATCCC = 86 reads: +291 -1XX +117 invalidated
umi TCATCATGAT = 304 reads: +291 -1XX +117 invalidated
umi TCCATCAGAC = 311 reads: +409 validated
umi TCCCCACGCC = 313 reads: +291 -1XX +117 invalidated
umi TCCCTGTGCA = 260 reads: +409 validated
umi TCGATTAGGC = 254 reads: +409 validated
umi TCTACGCCGG = 160 reads: +291 -1XX +117 invalidated
umi TCTTGGTAAT = 872 reads: -151X +140 -1XX +117 invalidated
umi TGACAGTCTT = 141 reads: +384 -25 non-validated
umi TGGATATCAC = 185 reads: +291 -1XX +115 -1 +1 invalidated
umi TGGTCGGCAC = 103 reads: +387 -22 non-validated
umi TGTCGACATA = 403 reads: +291 -1XX +117 invalidated
umi TGTGCGTCGC = 181 reads: +291 -1XX +117 invalidated
umi TGTTTTTTGC = 176 reads: +409 validated
umi TTACGACCTC = 237 reads: +409 validated
umi TTATATAGGT = 207 reads: +291 -1XX +117 invalidated
umi TTCAGATCCG = 204 reads: +409 validated
umi TTCTGTGGTT = 90 reads: +409 validated
umi TTGCATGAAC = 203 reads: +409 validated
umi TTGGAGCGTC = 134 reads: +394 -1 +14 non-validated
umi TTTCCAATCT = 324 reads: +409 validated

UMI info for barcode ACATCAGTCTTTACGT-1 contig 2 = GAGCTACAAC...
umi AACACGGGAG = 655 reads: -358 +1 -7X +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi AAGACCGGAG = 278 reads: +400 validated
umi AATTGTATTG = 323 reads: +400 validated
umi ACACATCAGA = 325 reads: -366X +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi ACACTTATAG = 290 reads: +400 validated
umi ACCCCAGCGG = 224 reads: +400 validated
umi ACCGTTACAA = 326 reads: +400 validated
umi ACGAGGCTCT = 325 reads: +400 validated
umi ACGAGTTGCT = 284 reads: +400 validated
umi ACTCTTCTGC = 300 reads: +400 validated
umi ACTTGTTCAG = 767 reads: -358 +1 -7XX +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi ACTTTCCGTG = 233 reads: +400 validated
umi AGAGTTTGGA = 258 reads: +400 validated
umi AGCAGCGAGC = 304 reads: +400 validated
umi AGTCTTTTTG = 112 reads: +400 validated
umi ATCGTCCTCG = 281 reads: +400 validated
umi ATCTATCAGC = 302 reads: +400 validated
umi ATGAGAGCTA = 345 reads: -366X +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi ATGGTCCTGG = 245 reads: +400 validated
umi ATGTGTAACT = 321 reads: +400 validated
umi CAAGGTAGAC = 371 reads: +400 validated
umi CACAGATTCT = 255 reads: +400 validated
umi CACCTTCCTG = 304 reads: +400 validated
umi CACTCACGCT = 167 reads: +400 validated
umi CATCGTTCCA = 317 reads: +400 validated
umi CATTTGTCAG = 267 reads: -358X +1 -6X +6 -1XX +4 -2XX +8 -1XX +5 -1XX +7 invalidated
umi CCACTCCCTT = 8 reads: -352 +1 -3XX +1 -2XX +1 -5XX +2 -1XX +19 -1XX +12 invalidated
umi CCGCTTCAGA = 286 reads: +400 validated
umi CCTCTAAATG = 206 reads: -358X +1 -4XX +1 -1XX +6 -1XX +3 -3XX +22 invalidated
umi CCTTCACCGT = 227 reads: +400 validated
umi CGCCACACTC = 275 reads: +400 validated
umi CGCCGCTGCT = 238 reads: +400 validated
umi CGGCGCTTTA = 225 reads: -366X +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi CTACGGTCCA = 165 reads: +400 validated
umi CTCTTACTGT = 288 reads: +400 validated
umi CTGCGCACTG = 327 reads: +400 validated
umi GCATTACGCA = 180 reads: +400 validated
umi GCCAATTCAG = 235 reads: +400 validated
umi GCTACCGGCC = 204 reads: +400 validated
umi GGACAAGACT = 237 reads: +400 validated
umi GGGCAACACT = 352 reads: -59 +341 non-validated
umi GGGGCTAATC = 271 reads: +400 validated
umi GTATGTGTAT = 278 reads: +400 validated
umi GTCATATGGG = 1 reads: -388 +4 -1X +7 invalidated
umi GTCCCAGCTT = 236 reads: +400 validated
umi GTCTTCGGCT = 252 reads: +400 validated
umi GTGACAACTG = 280 reads: +400 validated
umi GTGCAGTACG = 270 reads: +400 validated
umi TAAACACGCA = 219 reads: +400 validated
umi TAACACATTG = 178 reads: +400 validated
umi TAATACCTCA = 248 reads: +400 validated
umi TAATACGGCG = 301 reads: +400 validated
umi TACAAACTCT = 205 reads: +400 validated
umi TACGCGGCTG = 279 reads: +400 validated
umi TATTTCCTCG = 238 reads: +400 validated
umi TCACGCTTGA = 367 reads: +400 validated
umi TCCGTTTGGG = 316 reads: +400 validated
umi TCTGAATCCA = 204 reads: +400 validated
umi TCTTGCTGAG = 253 reads: +400 validated
umi TGAAATGTGC = 238 reads: +400 validated
umi TGAGCGTATT = 290 reads: +400 validated
umi TGGTCGTCTC = 222 reads: +400 validated
umi TGTCCTGCTG = 411 reads: -92X +308 invalidated
umi TTCATGTCAA = 258 reads: +400 validated
umi TTCCCCCGCT = 268 reads: +333 -2XX +1 -1XX +63 invalidated
umi TTCCGTTAGT = 260 reads: +400 validated
umi TTCTCAGTTA = 395 reads: -349X +1 -4X +2 -2X +1 -7XX +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi TTCTCCAGTG = 240 reads: +400 validated
umi TTCTTCTAAA = 318 reads: +400 validated
umi TTCTTTACAC = 558 reads: -358X +1 -7XX +2 -1XX +2 -1XX +5 -1XX +14 -1XX +7 invalidated
umi TTTTAGGCAA = 183 reads: +400 validated

GOOD CONTIGS

TIG 1[bases=543]
63-416 ==> 0-353 on |736|IGHV1-8|L-REGION+V-REGION| [len=353] (mis=14)
434-472 ==> 8-46 on |54|IGHJ4|J-REGION| [len=46] (mis=1)
472-543 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 75 umis using 1373 reads
cdr3 = CATGLLGGPIYW at 405, score = 10 + 7
umis assigned: [43, 122, 316, 342, 429, 435, 494, 550, 554, 594] and 88 others
of which 98 are surviving nonsolos
reads assigned: 22339
start codons at 63, 214, 261, 266, 270, 298, 327, 345, 360
confident = true

TIG 2[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=12)
391-430 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 60 umis using 2201 reads
cdr3 = CQHYYSIPPTF at 369, score = 9 + 8
umis assigned: [65, 130, 235, 278, 290, 352, 371, 408, 411, 503] and 61 others
of which 71 are surviving nonsolos
reads assigned: 19378
start codons at 30, 99, 352, 472
confident = true

REJECT CONTIGS

TIG 1[bases=636]
0-342 ==> 10-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=0)
340-391 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
391-636 ==> 0-245 on rc of segment before IGHJ5 exon 1 [len=348] (mis=6)
umis assigned: [1507, 2318]
of which 2 are surviving nonsolos
reads assigned: 129
start codons at 113, 161, 233, 335, 515, 553
confident = false
did not find CDR3

TIG 2[bases=788]
19-70 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
70-418 ==> 0-348 on rc of segment before IGHJ5 exon 1 [len=348] (mis=16)
468-625 ==> 0-157 on rc of segment before IGHJ3P exon 1 [len=157] (mis=0)
686-788 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
umis assigned: [2394, 3132, 3168, 4052]
of which 4 are surviving nonsolos
reads assigned: 1740
start codons at 194, 232, 475, 509, 548, 603, 643, 704, 765
confident = false
did not find CDR3
now this is a cell
paired!

AGCCTGACACCTGAGGACACGGCCGTCTATTATTGTGCGACAGGCCTCCTAGGGGGGCCAATCTACTGGGGCCAGGGAACCCCGGTCACCGTCTCCTCAG <==> ATCAGCAGCCTGCAGGCTGAAGATGTGGCAGTTTATTACTGTCAGCACTATTATTCTATTCCTCCCACTTTTGGCCAGGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.163 = ACATGGTAGCATCATC-1

using 285 reads

====================================================================================

graph has 144 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[3, 281]
surviving nonsolo ucounts = 1[281]
ids = [2]

====================================================================================

UMI info for barcode ACATGGTAGCATCATC-1 contig 1 = GGCTGGGGTC...
umi AGCATAAGCT = 1 reads: -388 non-validated
umi GCCCGCTTAT = 277 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=564]
42-391 ==> 0-349 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=3)
392-430 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=4)
430-564 ==> 0-134 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 49 reads
cdr3 = CSSYTSSSMGVF at 366, score = 8 + 8
umis assigned: [0, 2]
of which 1 are surviving nonsolos
reads assigned: 274
start codons at 42, 199, 243, 250, 253, 390
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.168 = ACATGGTAGCGTTGCC-1

using 249 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[249]
surviving nonsolo ucounts = 1[249]
ids = [0]

====================================================================================

UMI info for barcode ACATGGTAGCGTTGCC-1 contig 1 = AGCTTCAGCT...
umi TCCATATTCA = 239 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=571]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=1)
400-438 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
438-571 ==> 0-133 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CAAWDDSLNGHYVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 237
start codons at 47, 351, 376, 381, 393, 402
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.170 = ACATGGTAGCTTCGCG-1

using 92 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[92]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.173 = ACATGGTAGGACTGGT-1

using 27 reads

====================================================================================

graph has 16 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[27]
surviving nonsolo ucounts = 1[27]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.177 = ACATGGTAGGGTCGAT-1

using 340 reads

====================================================================================

graph has 126 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2^2, 3, 11, 18, 299]
surviving nonsolo ucounts = 1[299]
ids = [6]

====================================================================================

UMI info for barcode ACATGGTAGGGTCGAT-1 contig 1 = GAAGAGCTGC...
umi TGCGTCCTAA = 301 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=554]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=13)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYGRSPGTF at 357, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 297
start codons at 33, 241, 367, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.178 = ACATGGTAGGTGACCA-1

using 164 reads

====================================================================================

graph has 186 edges initially, 4 edges after simplification

total ucounts = 29
nonsolo ucounts = 23[2^4, 3^3, 4^2, 5^3, 9^4, 10^3, 12^2, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.179 = ACATGGTAGGTGATAT-1

using 8145 reads

====================================================================================

graph has 6715 edges initially, 70 edges after simplification

total ucounts = 1669
nonsolo ucounts = 867[2^347, 3^163, 4^130, 5^82, 6^43, 7^29, 8^22, 9^10, 10^7, 11^7, 12^3, 13^3, 14, 15^2, 16^2, 39, 41, 75, 122, 223, 245, 279, 282, 293, 303, 347, 350, 373, 384, 401, 447]
surviving nonsolo ucounts = 17[2^2, 39, 41, 75, 122, 223, 245, 279, 282, 293, 303, 347, 350, 373, 384, 401]
ids = [144, 1540, 1438, 380, 376, 78, 1237, 320, 918, 1638, ...]

====================================================================================

UMI info for barcode ACATGGTAGGTGATAT-1 contig 1 = ATCACATAAC...
umi AATTCGATCG = 124 reads: -86X +1 -2X +353 invalidated
umi ATTGAACTGA = 41 reads: +212 -5 +166 -1 +15 -1 +1 -41 non-validated
umi GAGTCTGTAA = 349 reads: -200 +242 non-validated
umi TACGCTCCTT = 223 reads: +442 validated
umi TGCAATATAC = 21 reads: -442 non-validated

UMI info for barcode ACATGGTAGGTGATAT-1 contig 2 = GGGAGAGCCC...
umi ATCCTCCTCT = 259 reads: +247 -1XX +137 invalidated
umi ATCTATTGTT = 299 reads: +385 validated
umi ATGCCAACGG = 384 reads: +385 validated
umi ATTCTAGGAG = 74 reads: +381 -1 +3 non-validated
umi ATTTTTGCGT = 292 reads: +385 validated
umi CTACCAGATC = 400 reads: +385 validated
umi GATCTTACTT = 282 reads: +385 validated
umi TCTATACTGG = 383 reads: +49 -5XX +1 -2XX +1 -6XX +1 -4XX +1 -2XX +1 -13X +1 -3XX +2 -4XX +1 -1XX +1 -3XX +283 invalidated
umi TGTAGCGTGG = 193 reads: -342 +1 -8XX +2 -1XX +6 -2XX +15 -1XX +7 invalidated
umi TTCCCCCCGT = 353 reads: +385 validated
umi TTTCATGGGT = 275 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=682]
0-58 ==> 0-58 on |87|IGHV1-69D|5'UTR| [len=58] (mis=0)
58-411 ==> 0-353 on |88|IGHV1-69D|L-REGION+V-REGION| [len=353] (mis=33)
457-500 ==> 20-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
500-682 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 3 umis using 151 reads
cdr3 = CARDSITMGHGGIKTANYGMDVW at 400, score = 8 + 7
umis assigned: [78, 380, 909, 1237, 1438]
of which 5 are surviving nonsolos
reads assigned: 745
start codons at 58, 209, 256, 322, 344, 421, 452, 457, 481
confident = true

TIG 2[bases=568]
0-47 ==> 0-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=2)
47-392 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=6)
395-432 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
432-568 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 10 umis using 445 reads
cdr3 = CQQRTNWPPLTF at 368, score = 9 + 9
umis assigned: [320, 329, 340, 376, 399, 732, 918, 1380, 1491, 1556] and 1 others
of which 10 are surviving nonsolos
reads assigned: 3121
start codons at 47, 252, 255, 474
confident = true
now this is a cell
paired!

TGTGCGAGGGATTCCATTACTATGGGTCACGGAGGTATTAAAACGGCTAACTATGGAATGGACGTCTGGGGCCAAGGGACCATGGTCACCGTCTCCTCAG <==> AGCAACCTAGAGCCTGAAGATTTTGCAGTTTATTACTGTCAGCAGCGTACCAACTGGCCTCCTCTCACTTTCGGCGGAGGGACCAAGGTGGAGATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.181 = ACATGGTAGTATCGAA-1

using 338 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 333]
surviving nonsolo ucounts = 1[333]
ids = [2]

====================================================================================

UMI info for barcode ACATGGTAGTATCGAA-1 contig 1 = GGAGTCAGAC...
umi GTTCAGCGCT = 329 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=478]
0-26 ==> 21-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
26-377 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=0)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-478 ==> 0-64 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CQQANSFPLTF at 353, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 320
start codons at 26, 32, 88, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.182 = ACATGGTAGTTAGGTA-1

using 2270 reads

====================================================================================

graph has 3362 edges initially, 32 edges after simplification

total ucounts = 936
nonsolo ucounts = 464[2^157, 3^110, 4^68, 5^37, 6^35, 7^16, 8^23, 9^9, 10^2, 11^4, 12, 17^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.184 = ACATGGTAGTTGAGTA-1

using 577 reads

====================================================================================

graph has 748 edges initially, 10 edges after simplification

total ucounts = 291
nonsolo ucounts = 123[2^55, 3^35, 4^11, 5^9, 6^5, 7^3, 8, 9, 11, 13^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.188 = ACATGGTCAAGACGTG-1

using 583 reads

====================================================================================

graph has 215 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[273, 307]
surviving nonsolo ucounts = 2[273, 307]
ids = [4, 3]

====================================================================================

UMI info for barcode ACATGGTCAAGACGTG-1 contig 1 = GAGGAATCAG...
umi GAAGTGGATG = 298 reads: +388 validated

UMI info for barcode ACATGGTCAAGACGTG-1 contig 2 = GCTGTGGGTC...
umi TCATTACTTA = 262 reads: +373 validated

GOOD CONTIGS

TIG 1[bases=502]
28-370 ==> 0-342 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=5)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-502 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CLQYNLNVWTF at 355, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 292
start codons at 28, 34, 103, 185, 239, 458
confident = false

TIG 2[bases=555]
0-38 ==> 5-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
38-375 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=7)
373-411 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
411-555 ==> 0-144 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQSPDSQGVF at 353, score = 8 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 38, 99, 168, 186
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.190 = ACATGGTCAAGCTGTT-1

using 23 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[23]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.192 = ACATGGTCAATCCAAC-1

using 992 reads

====================================================================================

graph has 288 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[4, 6, 978]
surviving nonsolo ucounts = 1[978]
ids = [6]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.200 = ACATGGTCACCGAAAG-1

using 425 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2, 3^2, 4, 7, 164, 237]
surviving nonsolo ucounts = 2[164, 237]
ids = [0, 6]

====================================================================================

UMI info for barcode ACATGGTCACCGAAAG-1 contig 1 = ACCCAGACGG...
umi AGTTGACGTT = 148 reads: +385 validated

UMI info for barcode ACATGGTCACCGAAAG-1 contig 2 = GGGGGCTGGG...
umi GGTTATCTTA = 231 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=422]
14-359 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=1)
361-399 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
399-422 ==> 0-23 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQYNNWPPITF at 335, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 147
start codons at 14, 83, 219
confident = false

TIG 2[bases=528]
45-406 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=11)
401-439 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
439-528 ==> 0-89 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CSSYTTSSTLLYVF at 369, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 230
start codons at 45, 202, 246, 253, 256, 403
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.202 = ACATGGTCACGGTGTC-1

using 252 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[248]
surviving nonsolo ucounts = 1[248]
ids = [0]

====================================================================================

UMI info for barcode ACATGGTCACGGTGTC-1 contig 1 = GGAAACAGAG...
umi AATTAGCAAG = 246 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=564]
39-391 ==> 0-352 on |333|IGLV10-54|L-REGION+V-REGION| [len=352] (mis=22)
389-427 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
427-564 ==> 0-137 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CSSWDSSLSAWVF at 360, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 241
start codons at 39, 178, 250, 368
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.207 = ACATGGTCAGATCCAT-1

using 202 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[197]
surviving nonsolo ucounts = 1[197]
ids = [3]

====================================================================================

UMI info for barcode ACATGGTCAGATCCAT-1 contig 1 = GTCAGTCTCA...
umi CGTTCCTTCG = 195 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=547]
0-23 ==> 24-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
23-374 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=30)
373-411 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=2)
junction support: 1 umis using 27 reads
cdr3 = CQQSDRTPITF at 350, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 192
start codons at 23, 29, 85, 98, 173, 234, 282, 333, 453
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.214 = ACATGGTCAGCTGCAC-1

using 142 reads

====================================================================================

graph has 50 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[2, 7, 127]
surviving nonsolo ucounts = 1[127]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTCAGCTGCAC-1 contig 1 = GTCAGGACAC...
umi ACCTTAGCTT = 107 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=469]
13-366 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=11)
364-401 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
401-469 ==> 0-68 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CQQSYTTLLTF at 340, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 106
start codons at 13, 19, 75, 88, 224, 443
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.216 = ACATGGTCAGGAACGT-1

using 4435 reads

====================================================================================

graph has 2812 edges initially, 54 edges after simplification

total ucounts = 707
nonsolo ucounts = 354[2^137, 3^65, 4^59, 5^24, 6^19, 7^12, 8^11, 9^5, 10^3, 11^4, 12^2, 13, 14, 23, 129, 174, 178, 218, 246, 336, 360, 368, 383, 386]
surviving nonsolo ucounts = 10[129, 174, 178, 218, 246, 336, 360, 368, 383, 386]
ids = [312, 667, 563, 127, 602, 67, 87, 74, 552, 688]

====================================================================================

UMI info for barcode ACATGGTCAGGAACGT-1 contig 1 = GAGAAGAGCT...
umi ACATTAATCG = 334 reads: +385 validated
umi ACCCGACACG = 373 reads: +385 validated
umi ACGTACGCTA = 362 reads: +385 validated
umi AGTATGCCTG = 222 reads: +385 validated
umi CGCGGCTTTT = 129 reads: +385 validated
umi TAGTCTAGGG = 385 reads: +385 validated
umi TCGTCTGTTG = 245 reads: +385 validated
umi TTCATGGTTA = 177 reads: +385 validated
umi TTTAGTCCAG = 384 reads: +385 validated

UMI info for barcode ACATGGTCAGGAACGT-1 contig 2 = GCATTCGGTG...
umi TATCTCACGT = 181 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=556]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=6)
383-420 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 9 umis using 375 reads
cdr3 = CQQYGSSPLSF at 359, score = 9 + 8
umis assigned: [67, 74, 87, 127, 312, 552, 602, 667, 688]
of which 9 are surviving nonsolos
reads assigned: 2570
start codons at 35, 243, 369, 462
confident = true

TIG 2[bases=511]
37-390 ==> 0-353 on |128|IGHV3-33|L-REGION+V-REGION| [len=353] (mis=11)
416-464 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
464-511 ==> 0-47 on |40|IGHG1|C-REGION| [len=884] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CARDYWGVATIGTAFDYW at 379, score = 9 + 7
umis assigned: [563]
of which 1 are surviving nonsolos
reads assigned: 177
start codons at 37, 188, 193, 251, 254, 340
confident = true
now this is a cell
paired!

ACGGCTCTCTATTACTGTGCGAGAGACTACTGGGGAGTGGCTACGATTGGGACGGCGTTTGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCAGACTGGAGCCTGAAGATTTTGCAGTGTATTACTGTCAGCAGTATGGTAGCTCACCTCTTTCTTTCGGCGGAGGGACCAAGGTGGACATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.225 = ACATGGTCATACGCTA-1

using 135 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 128]
surviving nonsolo ucounts = 1[128]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTCATACGCTA-1 contig 1 = GGAACTGCTC...
umi ATTCTTCGTA = 122 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=420]
0-31 ==> 16-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
31-376 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=1)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
junction support: 1 umis using 16 reads
cdr3 = CQQRSNWPPALTF at 352, score = 9 + 9
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 120
start codons at 31, 236, 239
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.233 = ACATGGTCATGTCCTC-1

using 343 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 3, 334]
surviving nonsolo ucounts = 1[334]
ids = [5]

====================================================================================

UMI info for barcode ACATGGTCATGTCCTC-1 contig 1 = GAGTCAGACC...
umi TCGTCCGCGA = 339 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=555]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=10)
380-419 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQHYNSFFLRYIF at 352, score = 8 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 25, 31, 87, 100, 236, 332, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.243 = ACATGGTGTAGAGGAA-1

using 162 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[162]
surviving nonsolo ucounts = 1[162]
ids = [0]

====================================================================================

UMI info for barcode ACATGGTGTAGAGGAA-1 contig 1 = TGGGGTCTCA...
umi CCACAAGTAG = 160 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-39 ==> 3-42 on |336|IGLV2-11|5'UTR| [len=42] (mis=0)
39-367 ==> 0-328 on |337|IGLV2-11|L-REGION+V-REGION| [len=358] (mis=16)
389-427 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=3)
427-549 ==> 0-122 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 23 reads
cdr3 = CSSVAGSYSLVF at 363, score = 8 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 155
start codons at 39, 178, 196, 247, 250, 346
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.247 = ACATGGTGTATGAATG-1

using 395 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[394]
surviving nonsolo ucounts = 1[394]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTGTATGAATG-1 contig 1 = AGAGCCCTGG...
umi CTGCTTCCAG = 382 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=511]
0-44 ==> 3-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
44-389 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
388-426 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=4)
426-511 ==> 0-85 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 61 reads
cdr3 = CQQRSNWPPAF at 365, score = 9 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 372
start codons at 44, 249, 252, 468
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.250 = ACATGGTGTCAGAATA-1

using 365 reads

====================================================================================

graph has 186 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^6, 3^4, 4, 5, 7, 9^3, 11, 13, 270]
surviving nonsolo ucounts = 1[270]
ids = [15]

====================================================================================

UMI info for barcode ACATGGTGTCAGAATA-1 contig 1 = AGGAGTCAGA...
umi GTTCCTTGCT = 261 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=468]
0-27 ==> 20-47 on |218|IGKV1-12|5'UTR| [len=47] (mis=0)
27-378 ==> 0-351 on |219|IGKV1-12|L-REGION+V-REGION| [len=351] (mis=1)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
415-468 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 40 reads
cdr3 = CQQANSFPRTF at 354, score = 9 + 8
umis assigned: [15]
of which 1 are surviving nonsolos
reads assigned: 258
start codons at 27, 33, 89, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.254 = ACATGGTGTCGCTTCT-1

using 94 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[93]
surviving nonsolo ucounts = 1[93]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTGTCGCTTCT-1 contig 1 = AGAGCCCTGG...
umi TAACTAATTA = 1 reads: -179 +2 -1 +5 -1X +1 -1 +1 -1 +3 -1 +1 -1 +4 -1 +1 -1 +10 -3 +1 -1 +1 -1 +1 -1 +1 -1 +3 -1 +4 -145 invalidated
umi TCCCTAATTC = 81 reads: +379 validated

GOOD CONTIGS

TIG 1[bases=513]
0-44 ==> 3-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
44-389 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=20)
385-423 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=1)
423-513 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 14 reads
cdr3 = CQQRSNWITF at 365, score = 9 + 8
umis assigned: [0, 1]
of which 1 are surviving nonsolos
reads assigned: 82
start codons at 44, 249, 252, 465
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.261 = ACATGGTGTGGCAAAC-1

using 17 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.265 = ACATGGTGTGTATGGG-1

using 163 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[3, 4, 151]
surviving nonsolo ucounts = 1[151]
ids = [5]

====================================================================================

UMI info for barcode ACATGGTGTGTATGGG-1 contig 1 = CTTGGGAGAG...
umi CGAGTTGGGG = 149 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=498]
0-34 ==> 25-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
34-387 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
436-470 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
470-498 ==> 0-28 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 9 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 376, score = 7 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 148
start codons at 34, 232, 237, 254, 298, 331
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.266 = ACATGGTGTGTGCCTG-1

using 10 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.267 = ACATGGTGTGTTAAGA-1

using 304 reads

====================================================================================

graph has 66 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[303]
surviving nonsolo ucounts = 1[303]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTGTGTTAAGA-1 contig 1 = AGGAGTCAGA...
umi TCCCTTTCCT = 308 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=551]
0-33 ==> 20-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
33-378 ==> 0-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=22)
387-415 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
415-551 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQYYSYPRTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 301
start codons at 27, 33, 89, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.281 = ACATGGTTCAACACGT-1

using 248 reads

====================================================================================

graph has 70 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2^2, 241]
surviving nonsolo ucounts = 1[241]
ids = [0]

====================================================================================

UMI info for barcode ACATGGTTCAACACGT-1 contig 1 = AGGAGTCAGT...
umi AAATGTGATT = 214 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=472]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
374-412 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
412-472 ==> 0-60 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQQYDNLPTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 27, 33, 89, 102, 241, 364, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.283 = ACATGGTTCACTATTC-1

using 10 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.287 = ACATGGTTCAGCACAT-1

using 297 reads

====================================================================================

graph has 122 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[2, 289]
surviving nonsolo ucounts = 1[289]
ids = [5]

====================================================================================

UMI info for barcode ACATGGTTCAGCACAT-1 contig 1 = GAGGAATCAG...
umi CACTTCCGAA = 2 reads: -266X +1 -2X +3 -1 +2 -1 +4 -1 +3 -1 +7 -2X +2 -2X +8 -1 +9 -1 +1 -70 invalidated
umi GACTTCCGTA = 277 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=497]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-497 ==> 0-81 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [0, 5]
of which 1 are surviving nonsolos
reads assigned: 273
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.296 = ACATGGTTCATTGCGA-1

using 230 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 4[2^2, 4, 216]
surviving nonsolo ucounts = 1[216]
ids = [5]

====================================================================================

UMI info for barcode ACATGGTTCATTGCGA-1 contig 1 = GCTCTGCTTC...
umi CTTGGCATCA = 212 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=537]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
407-445 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
445-537 ==> 0-92 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CQSYDSSLSGLYVF at 375, score = 7 + 8
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 210
start codons at 51, 205, 208, 259, 358, 385, 409
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.303 = ACATGGTTCCTGTACC-1

using 411 reads

====================================================================================

graph has 264 edges initially, 6 edges after simplification

total ucounts = 56
nonsolo ucounts = 43[2^8, 3^3, 5^6, 6^6, 7^3, 8^4, 9^4, 10^2, 11, 12, 13^4, 123]
surviving nonsolo ucounts = 1[123]
ids = [50]

====================================================================================

UMI info for barcode ACATGGTTCCTGTACC-1 contig 1 = GAGAAGAGCT...
umi TCTTTTGGAT = 113 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=465]
0-35 ==> 17-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
35-383 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=3)
381-420 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
420-465 ==> 0-45 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CQQYGSSPVTF at 359, score = 9 + 8
umis assigned: [50]
of which 1 are surviving nonsolos
reads assigned: 111
start codons at 35, 243, 246, 369
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.305 = ACATGGTTCCTTTCTC-1

using 645 reads

====================================================================================

graph has 202 edges initially, 6 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[12, 315, 316]
surviving nonsolo ucounts = 2[315, 316]
ids = [4, 1]

====================================================================================

UMI info for barcode ACATGGTTCCTTTCTC-1 contig 1 = ACTGACTACC...
umi ATGACTCGTC = 314 reads: +379 validated

UMI info for barcode ACATGGTTCCTTTCTC-1 contig 2 = GAGGAACTGC...
umi TTCTACGACC = 319 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=548]
0-33 ==> 30-63 on |297|IGKV5-2|5'UTR| [len=63] (mis=0)
33-378 ==> 0-345 on |298|IGKV5-2|L-REGION+V-REGION| [len=345] (mis=1)
375-412 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=4)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CLQHDNFPLF at 354, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 310
start codons at 33, 123, 181, 184, 189, 292, 337, 364, 454
confident = false

TIG 2[bases=554]
0-33 ==> 64-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
33-378 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
381-418 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
418-554 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQYNNWPPATF at 354, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 313
start codons at 33, 102, 238, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.310 = ACATGGTTCGCTAGCG-1

using 10531 reads

====================================================================================

graph has 4736 edges initially, 56 edges after simplification

total ucounts = 760
nonsolo ucounts = 367[2^136, 3^71, 4^51, 5^24, 6^15, 7^9, 8^4, 9^7, 10^3, 11^6, 12, 14^2, 15, 18, 32, 33, 71, 81, 128, 138, 147^2, 148, 155, 170, 184, 193, 201, 204, 225, 228, 234, 259, 261, 276, 295, 297, 303, 308, 329, 341, 349, 352, 356, 362, 382, 407, 418, 445, 453]
surviving nonsolo ucounts = 34[32, 81, 128, 138, 147^2, 148, 155, 170, 184, 193, 201, 204, 225, 228, 234, 259, 261, 276, 295, 297, 303, 308, 329, 341, 349, 352, 356, 362, 382, 407, 418, 445, 453]
ids = [368, 621, 157, 36, 117, 631, 679, 623, 480, 342, ...]

====================================================================================

UMI info for barcode ACATGGTTCGCTAGCG-1 contig 1 = AGAGCTCTGG...
umi AAAAACCGTT = 358 reads: +379 validated
umi AATATCTGTC = 298 reads: +379 validated
umi CAAGGTCCGC = 226 reads: +359 -9 +11 non-validated
umi CATCTTGCCC = 292 reads: +379 validated
umi CATGAATTGC = 344 reads: +379 validated
umi CGCAAATCTG = 460 reads: +379 validated
umi CTCTACACAT = 452 reads: +379 validated
umi GCATTAGTCG = 330 reads: +379 validated
umi GCTTGAATCG = 359 reads: +379 validated
umi GGACTACTCA = 170 reads: +379 validated
umi GTCCGATCTA = 418 reads: +379 validated
umi GTTCCCCCCT = 308 reads: +379 validated
umi TACGTGTGTA = 357 reads: +379 validated
umi TCGAAGGTTC = 86 reads: +379 validated
umi TCGAGATTCC = 156 reads: +379 validated
umi TCGGCTCCGA = 147 reads: +379 validated
umi TTATGCTCTA = 357 reads: +379 validated

UMI info for barcode ACATGGTTCGCTAGCG-1 contig 2 = AGCTCTGGGA...
umi AAGAGCGTAT = 135 reads: +421 validated
umi ACACAAACCC = 226 reads: +421 validated
umi AGTTCCCTGG = 147 reads: +421 validated
umi CAACTTTAAC = 126 reads: +406 -2 +1 -1X +2 -1 +5 -2 +1 invalidated
umi CACGGATGGC = 197 reads: +421 validated
umi CTAGGCTAAT = 175 reads: +419 -2 non-validated
umi CTCACTTTGT = 220 reads: +421 validated
umi CTGAATCAAC = 32 reads: +250 -1 +140 -30 non-validated
umi GCCTGATGCA = 259 reads: +421 validated
umi GGACGCGCAA = 198 reads: +421 validated
umi TGTCCGTGCA = 143 reads: +421 validated
umi TTCAGAATCC = 195 reads: +421 validated

GOOD CONTIGS

TIG 1[bases=559]
0-44 ==> 53-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
44-389 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=12)
385-423 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 15 umis using 726 reads
cdr3 = CQQYNKWRTF at 365, score = 9 + 8
umis assigned: [0, 46, 160, 192, 193, 297, 363, 451, 476, 480] and 7 others
of which 17 are surviving nonsolos
reads assigned: 5030
start codons at 44, 113, 249, 465
confident = true

TIG 2[bases=611]
0-80 ==> 40-120 on |105|IGHV3-11|5'UTR| [len=120] (mis=0)
80-433 ==> 0-353 on |106|IGHV3-11|L-REGION+V-REGION| [len=353] (mis=20)
452-501 ==> 0-49 on |56|IGHJ5|J-REGION| [len=49] (mis=5)
501-611 ==> 0-110 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 9 umis using 182 reads
cdr3 = CARGYSDYDSTCLDPW at 422, score = 9 + 7
umis assigned: [36, 58, 117, 157, 175, 342, 350, 368, 462, 479] and 2 others
of which 12 are surviving nonsolos
reads assigned: 2017
start codons at 80, 236, 366, 383, 555
confident = true

REJECT CONTIGS

TIG 1[bases=543]
3-78 ==> 5665-5740 on segment before IGKV1OR2-6 exon 1 [len=5879] (mis=2)
27-80 ==> 0-53 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=0)
80-338 ==> 60-318 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=10) [SHIFT!]
370-407 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=5)
407-543 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [305, 401, 459, 515, 586]
of which 5 are surviving nonsolos
reads assigned: 1613
start codons at 27, 33, 82, 95, 231, 449
confident = false
did not find CDR3
now this is a cell
paired!

GAGGACACGGCCGTATATTACTGTGCGAGAGGCTACAGTGACTACGATTCAACTTGTCTCGACCCCTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAA <==> ACCATCAGCAACCTGCAGTCTGAAGACTTTGCACTTTATTACTGTCAGCAATATAATAAGTGGCGGACGTTCGGCCAAGGGACCAAGGTGGAAATCAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.316 = ACATGGTTCTACGAGT-1

using 209 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 7, 196]
surviving nonsolo ucounts = 1[196]
ids = [1]

====================================================================================

UMI info for barcode ACATGGTTCTACGAGT-1 contig 1 = ACCCAAAAAC...
umi AACAATTACA = 190 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=533]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
456-490 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
490-533 ==> 0-43 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 396, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 188
start codons at 54, 252, 257, 274, 318, 351
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.325 = ACATGGTTCTTGAGAC-1

using 310 reads

====================================================================================

graph has 86 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[2, 4, 301]
surviving nonsolo ucounts = 1[301]
ids = [2]

====================================================================================

UMI info for barcode ACATGGTTCTTGAGAC-1 contig 1 = GAGGAACTGC...
umi AGGGGACACC = 277 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=510]
0-33 ==> 64-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
33-378 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=5)
376-415 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
415-510 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 52 reads
cdr3 = CQQYNHWPCSF at 354, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 33, 102, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.327 = ACATGGTTCTTGTACT-1

using 8049 reads

====================================================================================

graph has 5786 edges initially, 46 edges after simplification

total ucounts = 1372
nonsolo ucounts = 698[2^246, 3^151, 4^95, 5^71, 6^50, 7^24, 8^15, 9^8, 10^6, 11^2, 12, 13^2, 17, 20, 26, 29, 33, 55, 82, 125, 134, 142, 166, 178, 182, 184, 191, 226, 228, 236, 241, 246, 265, 276, 293, 325, 332, 333, 349]
surviving nonsolo ucounts = 23[26, 29, 33, 55, 125, 134, 142, 166, 178, 182, 191, 226, 228, 236, 241, 246, 265, 276, 293, 325, 332, 333, 349]
ids = [347, 1245, 389, 368, 126, 609, 476, 207, 985, 308, ...]

====================================================================================

UMI info for barcode ACATGGTTCTTGTACT-1 contig 1 = ATACTTTCTG...
umi AATCGGAAGC = 193 reads: +436 validated
umi ATTCTATGTT = 185 reads: +436 validated
umi CAACATTTCT = 26 reads: +321 -1 +2 -1 +64 -1 +5 -1 +6 -1 +1 -2 +1 -2 +7 -1X +2 -1 +4 -1 +3 -1 +1 -1 +5 invalidated
umi CCGCCGAGCA = 109 reads: +40 -1X +2 -1XX +1 -1XX +310 -1 +9 -1 +2 -1 +1 -1 +1 -1X +1 -1 +12 -1X +24 -23 invalidated
umi CTCCTATTGC = 332 reads: +436 validated
umi CTCTCATCGA = 240 reads: +436 validated
umi GTGACACCTT = 280 reads: +436 validated
umi TACACCTACT = 176 reads: +436 validated
umi TGTAGTTCGA = 235 reads: +436 validated
umi TTACACAACG = 30 reads: +406 -30 non-validated
umi TTCCGTTATG = 293 reads: +436 validated

UMI info for barcode ACATGGTTCTTGTACT-1 contig 2 = AGAGCCCTGG...
umi AACCTCTTCG = 266 reads: +379 validated
umi ACCATAGAGT = 127 reads: +379 validated
umi ACTTCTTCTT = 231 reads: +379 validated
umi AGAGCATAAC = 155 reads: -350 +1 -14X +1 -1XX +1 -5XX +1 -3XX +1 -1XX invalidated
umi ATGGTCCTAG = 242 reads: +379 validated
umi CAGCCTTCAA = 34 reads: +379 validated
umi CTCCTCGCGA = 137 reads: +379 validated
umi CTTGAAACTT = 352 reads: +379 validated
umi GCCACTCACA = 317 reads: -349X +2 -14X +1 -1XX +1 -5XX +1 -3XX +1 -1XX invalidated
umi TAGATTTAGT = 322 reads: +379 validated
umi TGTAGGTCTG = 247 reads: +379 validated
umi TTCACGTTCT = 49 reads: +24 -1 +1 -5X +1 -2XX +2 -1XX +1 -5XX +1 -2XX +263 -70 invalidated

GOOD CONTIGS

TIG 1[bases=633]
0-37 ==> 27-64 on |191|IGHV4-4|5'UTR| [len=64] (mis=2)
37-390 ==> 0-353 on |192|IGHV4-4|L-REGION+V-REGION| [len=353] (mis=36)
427-473 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=7)
473-633 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 8 umis using 228 reads
cdr3 = CARVGRYFLRGTYRYRHLDYW at 379, score = 8 + 6
umis assigned: [86, 308, 347, 476, 608, 621, 917, 985, 1220, 1245] and 1 others
of which 11 are surviving nonsolos
reads assigned: 2059
start codons at 16, 37, 81, 301, 370, 454, 527
confident = true

TIG 2[bases=559]
0-44 ==> 3-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
44-377 ==> 0-333 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=22)
385-423 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=5)
423-559 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 8 umis using 301 reads
cdr3 = CHQRGNLWSF at 365, score = 9 + 6
umis assigned: [38, 126, 195, 207, 295, 389, 609, 659, 745, 1012] and 2 others
of which 11 are surviving nonsolos
reads assigned: 2439
start codons at 44, 249, 252, 416, 465
confident = true

REJECT CONTIGS

TIG 1[bases=571]
0-331 ==> 21-352 on rc of segment before IGHJ4 exon 1 [len=352] (mis=7)
329-380 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=0)
380-571 ==> 0-191 on rc of segment before IGHJ5 exon 1 [len=348] (mis=1)
umis assigned: [368, 1286]
of which 1 are surviving nonsolos
reads assigned: 56
start codons at 102, 150, 222, 324, 504, 542
confident = false
did not find CDR3
now this is a cell
paired!

TATTACTGTGCGAGAGTGGGTCGCTATTTCCTGCGGGGGACCTATCGTTACAGGCACTTGGACTATTGGGGCCAGGGAATCATGGTCACCGTTTCTTCAG <==> ACCATCAACAACCTAGAGCCTGAAGACTTTGGAGTTTATTACTGTCATCAGCGTGGCAATTTGTGGTCCTTCGGCCAAGGGACCAGGGTGGACATGAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.332 = ACCAGTAAGAATTGTG-1

using 7 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[7]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.336 = ACCAGTAAGAGTAAGG-1

using 620 reads

====================================================================================

graph has 310 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[8, 282, 328]
surviving nonsolo ucounts = 2[282, 328]
ids = [3, 0]

====================================================================================

UMI info for barcode ACCAGTAAGAGTAAGG-1 contig 1 = ACCCAAAAAC...
umi TGCCGTTGGA = 248 reads: +418 validated

UMI info for barcode ACCAGTAAGAGTAAGG-1 contig 2 = GGAGAAGAGC...
umi AAAACTTTAT = 302 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=514]
0-54 ==> 5-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=0)
54-407 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=21)
404-424 ==> 0-20 on |13|IGHD2-15|D-REGION| [len=31] (mis=3)
437-472 ==> 11-46 on |54|IGHJ4|J-REGION| [len=46] (mis=1)
472-514 ==> 0-42 on |60|IGHM|C-REGION| [len=1358] (mis=1)
junction support: 1 umis using 13 reads
cdr3 = CARTYCSGGTCYDGW at 396, score = 8 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 54, 205, 252, 257, 274, 318, 351, 430, 433
confident = false

TIG 2[bases=503]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=1)
386-424 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
424-503 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQYGSSLPITF at 360, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 295
start codons at 36, 244, 370, 466
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.340 = ACCAGTAAGGACTGGT-1

using 209 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[3, 204]
surviving nonsolo ucounts = 2[3, 204]
ids = [0, 3]

====================================================================================

UMI info for barcode ACCAGTAAGGACTGGT-1 contig 1 = GCTGGGGTCA...
umi CACACCTTTG = 3 reads: -4 +56 -39 +56 -90 +56 -93 non-validated
umi TAAGCCTTGG = 202 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=557]
0-41 ==> 121-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
41-381 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=29)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=6)
435-557 ==> 0-122 on |306|IGLC2|C-REGION| [len=317] (mis=3)
junction support: 1 umis using 37 reads
cdr3 = CCSEAGGGVPGLLF at 365, score = 7 + 9
umis assigned: [0, 3]
of which 2 are surviving nonsolos
reads assigned: 200
start codons at 41, 195
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.345 = ACCAGTAAGGCTAGGT-1

using 304 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[2, 3, 292]
surviving nonsolo ucounts = 1[292]
ids = [6]

====================================================================================

UMI info for barcode ACCAGTAAGGCTAGGT-1 contig 1 = GAATCAGTCC...
umi TACTATGTTG = 276 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-499 ==> 0-86 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 272
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.349 = ACCAGTAAGTAATCCC-1

using 177 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[177]
surviving nonsolo ucounts = 1[177]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=432]
0-35 ==> 110-145 on |193|IGHV4-59|5'UTR| [len=145] (mis=0)
0-35 ==> 4708-4743 on rc of segment before IGHVII-28-1 exon 1 [len=4743] (mis=0)
0-35 ==> 4692-4727 on rc of segment before IGHVII-30-21 exon 1 [len=4727] (mis=0)
0-35 ==> 3481-3516 on rc of segment before IGHV3-60 exon 2 [len=3516] (mis=0)
0-35 ==> 3550-3585 on rc of segment before IGHV3-62 exon 2 [len=3585] (mis=0)
35-385 ==> 0-350 on |194|IGHV4-59|L-REGION+V-REGION| [len=350] (mis=0)
411-432 ==> 19-40 on |58|IGHJ6|J-REGION| [len=61] (mis=0)
cdr3 = CARDG at 374, score = 9 + 4
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 35, 79, 384
confident = false
VJ delta = -12
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.350 = ACCAGTAAGTACGCGA-1

using 346 reads

====================================================================================

graph has 110 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[343]
surviving nonsolo ucounts = 1[343]
ids = [3]

====================================================================================

UMI info for barcode ACCAGTAAGTACGCGA-1 contig 1 = GAATCAGTCC...
umi TTTGCCCTCA = 346 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
0-25 ==> 2-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
25-376 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CLQYSSSPWTF at 352, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 339
start codons at 25, 31, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.355 = ACCAGTACAACACGCC-1

using 26 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.363 = ACCAGTACACAACGTT-1

using 198 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 191]
surviving nonsolo ucounts = 1[191]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=378]
0-108 ==> 46-154 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=3)
108-304 ==> 155-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=14)
303-341 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
341-378 ==> 0-37 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CLQYSSSPWTF at 280, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 156
start codons at 29, 164
confident = false
not full
VJ delta = 15
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.364 = ACCAGTACACACAGAG-1

using 13 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.367 = ACCAGTACACCAGTTA-1

using 309 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[2^2, 3, 295]
surviving nonsolo ucounts = 1[295]
ids = [6]

====================================================================================

UMI info for barcode ACCAGTACACCAGTTA-1 contig 1 = AGTCAGACCC...
umi GTGACAGGCC = 277 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
0-24 ==> 157-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
24-359 ==> 0-335 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=13)
373-412 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=6)
412-499 ==> 0-87 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYYTYPYAF at 351, score = 8 + 7
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 275
start codons at 24, 30, 86, 99, 331, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.370 = ACCAGTACACGCCAGT-1

using 126 reads

====================================================================================

graph has 158 edges initially, 2 edges after simplification

total ucounts = 23
nonsolo ucounts = 19[2^2, 3^3, 4, 5, 6^3, 7^2, 8^4, 10, 12, 14]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.372 = ACCAGTACACGTTGGC-1

using 1112 reads

====================================================================================

graph has 354 edges initially, 4 edges after simplification

total ucounts = 5
nonsolo ucounts = 4[2, 3, 350, 756]
surviving nonsolo ucounts = 2[350, 756]
ids = [0, 2]

====================================================================================

UMI info for barcode ACCAGTACACGTTGGC-1 contig 1 = GGAGGAACTG...
umi CCTGATCCAA = 327 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=514]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
419-514 ==> 0-95 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYNNWPLWTF at 355, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 321
start codons at 34, 103, 239, 461
confident = false

REJECT CONTIGS

TIG 1[bases=436]
3-199 ==> 157-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=13)
228-276 ==> 13-61 on |58|IGHJ6|J-REGION| [len=61] (mis=2)
276-436 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
cdr3 = CARGDRRAAAFYYHYMDAW at 188, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 744
start codons at 44, 110, 143, 233, 330
confident = false
VJ delta = 21
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.375 = ACCAGTACAGAAGCAC-1

using 1066 reads

====================================================================================

graph has 382 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 3[4, 322, 737]
surviving nonsolo ucounts = 2[322, 737]
ids = [5, 2]

====================================================================================

REJECT CONTIGS

TIG 1[bases=556]
0-36 ==> 16-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
0-36 ==> 5964-6000 on rc of segment after IGKV3-20 exon 1 [len=6000] (mis=0)
36-384 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=0)
382-420 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=5)
420-556 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [2, 5]
of which 2 are surviving nonsolos
reads assigned: 1051
start codons at 36, 244, 370, 462
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.380 = ACCAGTACAGGCGATA-1

using 417 reads

====================================================================================

graph has 150 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[416]
surviving nonsolo ucounts = 1[416]
ids = [1]

====================================================================================

UMI info for barcode ACCAGTACAGGCGATA-1 contig 1 = CTGGGCCTCA...
umi TCGTACGCGG = 414 reads: +376 validated

GOOD CONTIGS

TIG 1[bases=624]
0-37 ==> 15-52 on |348|IGLV3-1|5'UTR| [len=52] (mis=0)
37-371 ==> 0-334 on |349|IGLV3-1|L-REGION+V-REGION| [len=346] (mis=18)
375-413 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=4)
413-624 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 76 reads
cdr3 = CQAWDSTEVVF at 352, score = 6 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 408
start codons at 37, 42, 331, 335
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.386 = ACCAGTACATGCATGT-1

using 398 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[395]
surviving nonsolo ucounts = 1[395]
ids = [1]

====================================================================================

UMI info for barcode ACCAGTACATGCATGT-1 contig 1 = GAGTCAGTCT...
umi ACCCCGACCG = 389 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=549]
25-378 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=17)
375-413 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
413-549 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQSYSTPWQF at 352, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 387
start codons at 25, 31, 87, 100, 236, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.388 = ACCAGTACATGGTAGG-1

using 470 reads

====================================================================================

graph has 146 edges initially, 4 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[75, 394]
surviving nonsolo ucounts = 2[75, 394]
ids = [1, 0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=488]
0-61 ==> 243-304 on |78|IGHV1-46|5'UTR| [len=304] (mis=1)
0-79 ==> 10061-10140 on rc of segment before IGHV1-18 exon 2 [len=10140] (mis=11)
61-414 ==> 0-353 on |79|IGHV1-46|L-REGION+V-REGION| [len=353] (mis=20)
443-488 ==> 13-58 on |58|IGHJ6|J-REGION| [len=61] (mis=1)
cdr3 = CARGDRRAAAFYYHYMDVW at 403, score = 8 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 68
start codons at 61, 217, 259, 325, 358, 448
confident = false
VJ delta = 21
not full
not full

TIG 2[bases=505]
0-32 ==> 21-53 on |236|IGKV1-8|5'UTR| [len=53] (mis=1)
0-32 ==> 5968-6000 on rc of segment after IGKV1-8 exon 1 [len=6000] (mis=1)
13-83 ==> 8897-8967 on segment before IGKV1OR2-3 exon 1 [len=9099] (mis=6)
13-83 ==> 8906-8976 on rc of segment before IGKV2OR2-10 exon 1 [len=9109] (mis=6)
13-83 ==> 8905-8975 on segment before IGKV1OR2-11 exon 1 [len=9108] (mis=6)
32-247 ==> 0-215 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=14)
248-378 ==> 215-345 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=8) [SHIFT!]
387-415 ==> 11-39 on |214|IGKJ2|J-REGION| [len=39] (mis=1)
415-505 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CQQYYSYPRTF at 354, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 355
start codons at 26, 32, 88, 101, 237, 457
confident = false
not full
frameshifted full length stopped transcript of length 505
VJ delta = 14
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.389 = ACCAGTACATTAGCCA-1

using 277 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 270]
surviving nonsolo ucounts = 1[270]
ids = [2]

====================================================================================

UMI info for barcode ACCAGTACATTAGCCA-1 contig 1 = GCTCTGCTTC...
umi CATGAACGCG = 264 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=591]
0-51 ==> 0-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
51-407 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
423-442 ==> 19-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
442-591 ==> 0-149 on |305|IGLC1|C-REGION| [len=317] (mis=1)
junction support: 1 umis using 49 reads
cdr3 = CQSYDSSLSGYVF at 375, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 261
start codons at 51, 205, 259, 358, 385, 406, 574
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.412 = ACCAGTAGTGCGCTTG-1

using 214 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 2[2, 207]
surviving nonsolo ucounts = 2[2, 207]
ids = [5, 4]

====================================================================================

UMI info for barcode ACCAGTAGTGCGCTTG-1 contig 1 = GGAGGAACTG...
umi TCAAGGCCTT = 194 reads: +385 validated
umi TCATCTTTCG = 2 reads: -153 +71 -161 non-validated

GOOD CONTIGS

TIG 1[bases=498]
0-34 ==> 63-97 on |280|IGKV3-15|5'UTR| [len=97] (mis=0)
34-379 ==> 0-345 on |281|IGKV3-15|L-REGION+V-REGION| [len=345] (mis=0)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
419-498 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CQQYNNWPLWTF at 355, score = 9 + 8
umis assigned: [4, 5]
of which 2 are surviving nonsolos
reads assigned: 190
start codons at 34, 103, 239, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.416 = ACCAGTAGTGTCTGAT-1

using 56 reads

====================================================================================

graph has 32 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 50]
surviving nonsolo ucounts = 1[50]
ids = [0]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.430 = ACCAGTATCACCACCT-1

using 454 reads

====================================================================================

graph has 128 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[2, 450]
surviving nonsolo ucounts = 1[450]
ids = [1]

====================================================================================

UMI info for barcode ACCAGTATCACCACCT-1 contig 1 = GAGGAATCAG...
umi GACAAAACAC = 448 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=552]
28-379 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=20)
378-416 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
416-552 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 65 reads
cdr3 = CLQYSSSPWTF at 355, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 443
start codons at 28, 34, 103, 239, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.432 = ACCAGTATCAGCTCGG-1

using 167 reads

====================================================================================

graph has 98 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[163]
surviving nonsolo ucounts = 1[163]
ids = [2]

====================================================================================

UMI info for barcode ACCAGTATCAGCTCGG-1 contig 1 = GATGCTTTCT...
umi TGAGCCAAAA = 157 reads: +457 validated

GOOD CONTIGS

TIG 1[bases=574]
17-394 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=34)
421-474 ==> 0-53 on |51|IGHJ2|J-REGION| [len=53] (mis=6)
474-574 ==> 0-100 on |40|IGHG1|C-REGION| [len=884] (mis=1)
junction support: 1 umis using 22 reads
cdr3 = CARPILARAGGMPAYFFDLW at 383, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 152
start codons at 1, 17, 26, 38, 82, 162, 179, 416
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.434 = ACCAGTATCATCGGAT-1

using 1563 reads

====================================================================================

graph has 2218 edges initially, 16 edges after simplification

total ucounts = 668
nonsolo ucounts = 314[2^111, 3^67, 4^51, 5^24, 6^27, 7^11, 8^11, 9^4, 10^3, 12^3, 14, 19]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.436 = ACCAGTATCATGTCCC-1

using 461 reads

====================================================================================

graph has 222 edges initially, 34 edges after simplification

total ucounts = 12
nonsolo ucounts = 8[2, 3^2, 4, 6, 59, 90, 290]
surviving nonsolo ucounts = 3[59, 90, 290]
ids = [2, 4, 5]

====================================================================================

UMI info for barcode ACCAGTATCATGTCCC-1 contig 1 = GAGAGAGGAG...
umi CAGACTTCCG = 59 reads: +359 -13 +16 -1X +35 invalidated
umi GTACGTTTCT = 91 reads: +424 validated

UMI info for barcode ACCAGTATCATGTCCC-1 contig 2 = AGCACTAGAA...
umi TAACCTCCCG = 256 reads: +430 validated

GOOD CONTIGS

TIG 1[bases=501]
0-73 ==> 6-79 on |119|IGHV3-21|5'UTR| [len=79] (mis=0)
73-426 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
450-497 ==> 16-63 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
junction support: 1 umis using 11 reads
cdr3 = CARDRAATARLGGMDVW at 415, score = 9 + 7
umis assigned: [2, 4]
of which 2 are surviving nonsolos
reads assigned: 147
start codons at 73, 224, 229, 376, 454
confident = false

TIG 2[bases=491]
0-60 ==> 20-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
60-411 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=10)
413-440 ==> 0-27 on |18|IGHD3-10|D-REGION| [len=27] (mis=4)
442-490 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
junction support: 1 umis using 16 reads
cdr3 = CAKGDYYGSGTYSPYFDYW at 402, score = 8 + 7
umis assigned: [5]
of which 1 are surviving nonsolos
reads assigned: 252
start codons at 60, 211, 216, 274, 277, 295, 363, 421
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.438 = ACCAGTATCCAAAGTC-1

using 336 reads

====================================================================================

graph has 118 edges initially, 2 edges after simplification

total ucounts = 6
nonsolo ucounts = 1[331]
surviving nonsolo ucounts = 1[331]
ids = [2]

====================================================================================

UMI info for barcode ACCAGTATCCAAAGTC-1 contig 1 = GGAGTCAGAC...
umi CGGGAGTCGT = 310 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=455]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=9)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
414-455 ==> 0-41 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 53 reads
cdr3 = CQQYNSYPLTF at 353, score = 8 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 306
start codons at 26, 32, 88, 101, 237, 240, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.439 = ACCAGTATCCAATGGT-1

using 24 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 7, 13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.442 = ACCAGTATCCCAGGTG-1

using 318 reads

====================================================================================

graph has 100 edges initially, 4 edges after simplification

total ucounts = 4
nonsolo ucounts = 2[128, 188]
surviving nonsolo ucounts = 2[128, 188]
ids = [0, 2]

====================================================================================

UMI info for barcode ACCAGTATCCCAGGTG-1 contig 1 = AGTCTGGGCC...
umi ACGTCTACTG = 126 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=492]
0-40 ==> 211-251 on |358|IGLV3-21|5'UTR| [len=251] (mis=0)
40-391 ==> 0-351 on |359|IGLV3-21|L-REGION+V-REGION| [len=351] (mis=5)
384-422 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=2)
422-492 ==> 0-70 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQVWDSSSDHQVF at 355, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 122
start codons at 40, 101, 239, 242, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.448 = ACCAGTATCGTTGACA-1

using 271 reads

====================================================================================

graph has 96 edges initially, 2 edges after simplification

total ucounts = 16
nonsolo ucounts = 5[2^3, 6, 248]
surviving nonsolo ucounts = 1[248]
ids = [7]

====================================================================================

REJECT CONTIGS

TIG 1[bases=353]
4-38 ==> 5966-6000 on rc of segment after IGHV4-4 exon 1 [len=6000] (mis=0)
17-171 ==> 0-154 on |184|IGHV4-34|L-REGION+V-REGION| [len=369] (mis=1)
171-353 ==> 0-182 on |40|IGHG1|C-REGION| [len=884] (mis=1)
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 240
start codons at 17, 38, 82, 168
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.466 = ACCCACTAGAGACTAT-1

using 316 reads

====================================================================================

graph has 164 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 5[2, 6, 7, 102, 198]
surviving nonsolo ucounts = 2[102, 198]
ids = [2, 0]

====================================================================================

UMI info for barcode ACCCACTAGAGACTAT-1 contig 1 = AGCTTCAGCT...
umi CCATACGGGC = 194 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=540]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-540 ==> 0-105 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 30 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 192
start codons at 47, 201, 351, 376, 381
confident = false

REJECT CONTIGS

TIG 1[bases=427]
10-363 ==> 0-353 on |120|IGHV3-21|L-REGION+V-REGION| [len=353] (mis=18)
387-427 ==> 16-56 on |737|IGHJ6|J-REGION| [len=63] (mis=1)
cdr3 = CARDRAATARLGGMDVW at 352, score = 9 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 89
start codons at 10, 161, 166, 313, 391
confident = false
VJ delta = 23
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.467 = ACCCACTAGAGGGCTT-1

using 178 reads

====================================================================================

graph has 174 edges initially, 10 edges after simplification

total ucounts = 38
nonsolo ucounts = 24[2^3, 3^6, 4^2, 5, 6^3, 8, 9^3, 11^2, 12, 14, 26]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.468 = ACCCACTAGAGTACAT-1

using 553 reads

====================================================================================

graph has 176 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[550]
surviving nonsolo ucounts = 1[550]
ids = [3]

====================================================================================

UMI info for barcode ACCCACTAGAGTACAT-1 contig 1 = TGGGGTCTCA...
umi GTGCCAGCTT = 557 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=638]
0-39 ==> 124-163 on |346|IGLV2-8|5'UTR| [len=163] (mis=0)
39-393 ==> 0-354 on |347|IGLV2-8|L-REGION+V-REGION| [len=354] (mis=13)
389-427 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=5)
427-638 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 74 reads
cdr3 = CSSHAGSTRVLF at 363, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 548
start codons at 39, 196, 247, 346, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.471 = ACCCACTAGATCCCGC-1

using 396 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[2, 393]
surviving nonsolo ucounts = 1[393]
ids = [0]

====================================================================================

UMI info for barcode ACCCACTAGATCCCGC-1 contig 1 = AGGTCTCAGA...
umi ACCCTTTTCG = 391 reads: +415 validated

GOOD CONTIGS

TIG 1[bases=565]
0-79 ==> 0-79 on |158|IGHV3-7|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |159|IGHV3-7|L-REGION+V-REGION| [len=353] (mis=14)
448-494 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
494-565 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CARGDPEYPGTDYW at 421, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 388
start codons at 79, 235, 296, 314, 382
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.476 = ACCCACTAGCGTCAAG-1

using 271 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 3, 264]
surviving nonsolo ucounts = 1[264]
ids = [4]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.488 = ACCCACTAGTATCTCG-1

using 3011 reads

====================================================================================

graph has 2506 edges initially, 36 edges after simplification

total ucounts = 639
nonsolo ucounts = 312[2^112, 3^73, 4^43, 5^30, 6^21, 7^14, 8^5, 9, 10^2, 11^3, 12, 30, 210, 214, 233, 285, 292, 317]
surviving nonsolo ucounts = 7[30, 210, 214, 233, 285, 292, 317]
ids = [388, 629, 396, 163, 510, 386, 429]

====================================================================================

UMI info for barcode ACCCACTAGTATCTCG-1 contig 1 = AGTGACTCCT...
umi ATTGCACAGT = 226 reads: +424 validated
umi GCGATGGTGA = 30 reads: +357 -10 +55 -2 non-validated

UMI info for barcode ACCCACTAGTATCTCG-1 contig 2 = GATCAGGACT...
umi GCGAAATCTC = 289 reads: +397 validated
umi GCTCGCTTCG = 213 reads: +397 validated
umi GGTCTATATG = 320 reads: +397 validated
umi TCATTCTGTT = 289 reads: +397 validated
umi TTTCCTCAGT = 221 reads: +160 -1XX +236 invalidated

GOOD CONTIGS

TIG 1[bases=520]
0-20 ==> 0-20 on |97|IGHV2-5|5'UTR| [len=20] (mis=0)
20-378 ==> 0-358 on |98|IGHV2-5|L-REGION+V-REGION| [len=358] (mis=13)
396-444 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=1)
444-520 ==> 0-76 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CAHRDSSDSLYYFGYW at 365, score = 7 + 7
umis assigned: [163, 388]
of which 2 are surviving nonsolos
reads assigned: 251
start codons at 20, 64, 108, 243, 249, 326, 335, 498
confident = true

TIG 2[bases=563]
0-30 ==> 0-30 on |274|IGKV2D-29|5'UTR| [len=30] (mis=0)
30-390 ==> 0-360 on |275|IGKV2D-29|L-REGION+V-REGION| [len=360] (mis=14)
390-427 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
427-563 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 5 umis using 180 reads
cdr3 = CMQSIQFPLTF at 366, score = 8 + 9
umis assigned: [386, 396, 429, 510, 629]
of which 5 are surviving nonsolos
reads assigned: 1304
start codons at 30, 63, 99, 187, 250, 349, 369, 469
confident = true
now this is a cell
paired!

GTGGACACAGCCACATATTACTGTGCACACAGGGATAGCAGTGACTCGCTATACTACTTTGGCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> ATCAGCCGGGTGGAGGCTGAGGATGTTGGGAATTATTACTGCATGCAAAGTATACAGTTTCCCCTCACGTTCGGCGGAGGGACCAGGGTGGAGATCAATC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.498 = ACCCACTCAAACCCAT-1

using 232 reads

====================================================================================

graph has 104 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[225]
surviving nonsolo ucounts = 1[225]
ids = [7]

====================================================================================

UMI info for barcode ACCCACTCAAACCCAT-1 contig 1 = AGGAGTCAGA...
umi TCGGGACCAT = 214 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=474]
0-27 ==> 154-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
27-378 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=17)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=3)
415-474 ==> 0-59 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CQQYNSYALSF at 354, score = 8 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 213
start codons at 27, 33, 89, 102, 238, 241, 334, 373, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.500 = ACCCACTCAAGCCGCT-1

using 25 reads

====================================================================================

graph has 22 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[25]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.502 = ACCCACTCAAGTTAAG-1

using 230 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 6, 215]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=464]
1-38 ==> 4706-4743 on rc of segment before IGHVII-28-1 exon 1 [len=4743] (mis=1)
1-38 ==> 4690-4727 on rc of segment before IGHVII-30-21 exon 1 [len=4727] (mis=1)
1-38 ==> 3479-3516 on rc of segment before IGHV3-60 exon 2 [len=3516] (mis=1)
1-38 ==> 3548-3585 on rc of segment before IGHV3-62 exon 2 [len=3585] (mis=1)
38-386 ==> 0-348 on |740|IGHV4-30-4|L-REGION+V-REGION| [len=348] (mis=8)
420-464 ==> 0-44 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
432-464 ==> 13-45 on |56|IGHJ5|J-REGION| [len=49] (mis=0)
cdr3 = CAREGSGSGWYGIAFDSW at 383, score = 9 + 7
umis assigned: [4]
of which 0 are surviving nonsolos
reads assigned: 187
start codons at 38, 82
confident = false
VJ delta = 1
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.503 = ACCCACTCAATAAGCA-1

using 236 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 3, 224]
surviving nonsolo ucounts = 1[224]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.513 = ACCCACTCACCACCAG-1

using 16 reads

====================================================================================

graph has 20 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.515 = ACCCACTCACCAGTTA-1

using 24170 reads

====================================================================================

graph has 8657 edges initially, 66 edges after simplification

total ucounts = 1353
nonsolo ucounts = 658[2^240, 3^129, 4^69, 5^49, 6^21, 7^12, 8^10, 9^4, 10^4, 11^4, 12, 13^4, 14^2, 16, 17, 18^2, 20^2, 25, 28, 30, 39, 41, 47, 50^2, 61, 73, 76, 93, 95, 101^2, 102, 111, 113, 117, 119, 123, 124^2, 127, 133, 134, 153, 155^3, 160, 162, 163^2, 166, 168, 171, 175, 180, 181, 182, 183, 184, 187, 188, 190^2, 193, 195^2, 198, 199, 200^2, 201^2, 203, 207, 208, 210, 211^2, 212, 213, 215, 216^3, 218^2, 219, 220, 222, 224, 227^2, 228^2, 229, 231, 232, 233, 237, 239^2, 240, 242, 244, 246, 247, 253, 256, 261, 270, 293, 369, 384, 412, 424, 719, 740, 784, 1052]
surviving nonsolo ucounts = 95[50, 61, 73, 76, 93, 95, 101^2, 102, 111, 113, 117, 123, 124^2, 127, 133, 134, 153, 155^3, 160, 162, 163^2, 166, 168, 171, 175, 180, 181, 182, 183, 184, 187, 188, 190^2, 193, 195^2, 198, 199, 200^2, 201^2, 203, 207, 208, 210, 211^2, 212, 213, 215, 216^3, 218^2, 219, 220, 222, 224, 227^2, 228^2, 229, 231, 232, 233, 237, 239^2, 240, 242, 244, 246, 247, 253, 256, 261, 270, 293, 369, 384, 412, 424, 719, 740, 784, 1052]
ids = [242, 647, 311, 368, 663, 383, 100, 584, 508, 1264, ...]

====================================================================================

UMI info for barcode ACCCACTCACCAGTTA-1 contig 1 = AGCTTCAGCT...
umi AACTATTCAC = 227 reads: +388 validated
umi AAGAACTATG = 168 reads: +388 validated
umi AATCTCTCCG = 157 reads: +388 validated
umi ACACTTTATC = 254 reads: +388 validated
umi ACAGCTCTCC = 98 reads: +388 validated
umi ACCAGCCTCT = 410 reads: +388 validated
umi ACCTTGTCGG = 257 reads: +388 validated
umi ACTTCATTGC = 226 reads: +388 validated
umi AGCATTGTCC = 213 reads: +388 validated
umi AGTCCCGGAA = 218 reads: +388 validated
umi ATCACGTTCC = 206 reads: +388 validated
umi ATCCATTCCG = 205 reads: +388 validated
umi ATCCTTGTAG = 114 reads: +388 validated
umi ATGAATCTCC = 272 reads: +388 validated
umi ATTCATTCAA = 194 reads: +388 validated
umi ATTCTAAGTG = 64 reads: +304 -84 non-validated
umi CAAATGGGGT = 239 reads: +388 validated
umi CAACCTACGC = 246 reads: +388 validated
umi CACTCAGTCA = 95 reads: +339 -1 +48 non-validated
umi CAGTTGTCCA = 179 reads: +380 -8 non-validated
umi CATGTTAAAG = 155 reads: +388 validated
umi CCACCAGTCT = 225 reads: +388 validated
umi CCATTCCGAG = 220 reads: +388 validated
umi CGGGAGACGG = 194 reads: +388 validated
umi CGTATATGCA = 205 reads: +386 -1X +1 invalidated
umi CGTCGTTTAG = 103 reads: +388 validated
umi CTAAACCTTG = 136 reads: +388 validated
umi CTAACTGATA = 237 reads: +388 validated
umi CTATATGCCT = 199 reads: +388 validated
umi CTCTGCGGGT = 61 reads: +318 -6 +16 -1X +47 invalidated
umi CTGCGGCCGA = 96 reads: +341 -47 non-validated
umi CTTTCAGGCC = 217 reads: +388 validated
umi GACTCTATGT = 225 reads: +388 validated
umi GAGGTATCTT = 163 reads: +355 -13 +20 non-validated
umi GAGTACCTTT = 384 reads: +388 validated
umi GATTATTTAT = 239 reads: +388 validated
umi GCAGATTATG = 227 reads: +245 -5XX +1 -3XX +134 invalidated
umi GCCCGTTTTT = 245 reads: +388 validated
umi GCGCGACATC = 228 reads: +388 validated
umi GGGCTCGGTC = 724 reads: -192X +196 invalidated
umi GGGTGTCTTA = 251 reads: +388 validated
umi GTTGTTTGAT = 177 reads: +388 validated
umi TAATATAGGC = 199 reads: +388 validated
umi TACCAGGATC = 218 reads: +388 validated
umi TCACAGCTAC = 193 reads: +388 validated
umi TCATGTCTCC = 181 reads: +388 validated
umi TCCAATGAAT = 182 reads: +388 validated
umi TGAATTATTT = 226 reads: +388 validated
umi TGTCCTGTAC = 233 reads: +388 validated
umi TTAATGGCGG = 153 reads: +388 validated
umi TTAATTGATC = 196 reads: +388 validated
umi TTAGGTCTTT = 237 reads: +388 validated
umi TTTTAGTCTT = 205 reads: +388 validated
umi TTTTTTACTC = 116 reads: +388 validated

UMI info for barcode ACCCACTCACCAGTTA-1 contig 2 = TCGGTGATCA...
umi ACGGACCCAG = 789 reads: -209X +221 invalidated
umi CCTAGTGAGC = 99 reads: +430 validated
umi GAGATTACCC = 645 reads: -383X +1 -2XX +3 -1XX +1 -1XX +1 -2XX +9 -1XX +2 -1XX +3 -2XX +17 invalidated

GOOD CONTIGS

TIG 1[bases=646]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=2)
397-435 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
435-646 ==> 0-211 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 49 umis using 1371 reads
cdr3 = CAAWDDSLNGVVF at 368, score = 8 + 8
umis assigned: [33, 39, 64, 96, 100, 118, 146, 184, 194, 212] and 44 others
of which 54 are surviving nonsolos
reads assigned: 11119
start codons at 47, 351, 376, 381, 393
confident = true

TIG 2[bases=565]
0-33 ==> 47-80 on |125|IGHV3-33|5'UTR| [len=80] (mis=0)
33-384 ==> 0-351 on |126|IGHV3-33|L-REGION+V-REGION| [len=351] (mis=7)
417-463 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=5)
463-565 ==> 0-102 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 2 umis using 154 reads
cdr3 = CATDQLTRGAVLPGNIAYW at 375, score = 9 + 7
umis assigned: [155, 508, 724]
of which 3 are surviving nonsolos
reads assigned: 1511
start codons at 33, 184, 189, 247, 250, 268, 336, 481, 542
confident = true

REJECT CONTIGS

TIG 1[bases=646]
4-31 ==> 5785-5812 on segment before IGLV2-34 exon 1 [len=6000] (mis=0)
26-92 ==> 5809-5875 on segment before IGLV2-34 exon 1 [len=6000] (mis=3)
45-406 ==> 0-361 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=7)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=2)
435-646 ==> 0-211 on |307|IGLC3|C-REGION| [len=317] (mis=0)
umis assigned: [18, 38, 43, 92, 107, 121, 138, 156, 219, 242] and 28 others
of which 38 are surviving nonsolos
reads assigned: 8074
start codons at 45, 202, 246, 253, 256
confident = false
did not find CDR3
now this is a cell
paired!

GCTGTGTATTACTGTGCAACCGATCAACTCACACGGGGGGCTGTATTACCAGGCAATATCGCCTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GGGCTCCAGTCTGAGGATGAGGCTGATTATTACTGTGCAGCATGGGATGACAGCCTGAATGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.529 = ACCCACTCAGCTGGCT-1

using 526 reads

====================================================================================

graph has 148 edges initially, 6 edges after simplification

total ucounts = 12
nonsolo ucounts = 7[2^3, 3^2, 175, 334]
surviving nonsolo ucounts = 2[175, 334]
ids = [9, 4]

====================================================================================

UMI info for barcode ACCCACTCAGCTGGCT-1 contig 1 = GAGCTACAAC...
umi TCCGGGTTCC = 177 reads: +400 validated

UMI info for barcode ACCCACTCAGCTGGCT-1 contig 2 = GGAGTCAGTC...
umi GTAGTAAGCA = 334 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=566]
0-30 ==> 145-175 on |295|IGKV4-1|5'UTR| [len=175] (mis=0)
30-393 ==> 0-363 on |296|IGKV4-1|L-REGION+V-REGION| [len=363] (mis=4)
393-430 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=2)
430-566 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CQQYYSTSVTF at 369, score = 9 + 8
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 175
start codons at 30, 99, 352, 472
confident = false

TIG 2[bases=550]
0-26 ==> 5-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
26-377 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=29)
375-414 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=5)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CHQYESVPYTF at 353, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 332
start codons at 26, 32, 88, 101, 240, 336, 363, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.534 = ACCCACTCAGGTTTCA-1

using 135 reads

====================================================================================

graph has 54 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 1[129]
surviving nonsolo ucounts = 1[129]
ids = [2]

====================================================================================

UMI info for barcode ACCCACTCAGGTTTCA-1 contig 1 = CTGTGGGCAC...
umi GAAGATGCGT = 128 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=517]
0-39 ==> 12-51 on |320|IGLV1-40|5'UTR| [len=51] (mis=0)
39-395 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=12)
392-430 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
430-517 ==> 0-87 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CQSYDTSLSGSVF at 363, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 125
start codons at 39, 193, 196, 247, 346, 373
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.537 = ACCCACTCATAACCTG-1

using 13 reads

====================================================================================

graph has 6 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.541 = ACCCACTCATCCGTGG-1

using 304 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[3, 294]
surviving nonsolo ucounts = 1[294]
ids = [0]

====================================================================================

UMI info for barcode ACCCACTCATCCGTGG-1 contig 1 = GGGGAGGAAT...
umi AGCTCAAGAG = 292 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=555]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 37 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 288
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.551 = ACCCACTGTAAGAGGA-1

using 398 reads

====================================================================================

graph has 174 edges initially, 4 edges after simplification

total ucounts = 6
nonsolo ucounts = 4[2^2, 100, 292]
surviving nonsolo ucounts = 2[100, 292]
ids = [4, 2]

====================================================================================

UMI info for barcode ACCCACTGTAAGAGGA-1 contig 1 = AGCTTCAGCT...
umi CTCATCCATT = 291 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=579]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-579 ==> 0-144 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 284
start codons at 47, 201, 351, 376, 381
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.558 = ACCCACTGTATATGAG-1

using 1853 reads

====================================================================================

graph has 2116 edges initially, 16 edges after simplification

total ucounts = 705
nonsolo ucounts = 325[2^134, 3^68, 4^55, 5^26, 6^16, 7^6, 8^10, 9^3, 10, 11, 12, 13^2, 15, 332]
surviving nonsolo ucounts = 1[332]
ids = [367]

====================================================================================

UMI info for barcode ACCCACTGTATATGAG-1 contig 1 = GGAGGAATCA...
umi GCATGATTCT = 323 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=497]
29-380 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
379-417 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
417-497 ==> 0-80 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 48 reads
cdr3 = CLQYSSSPWTF at 356, score = 9 + 8
umis assigned: [367]
of which 1 are surviving nonsolos
reads assigned: 318
start codons at 29, 35, 104, 240, 459
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.564 = ACCCACTGTCATACTG-1

using 2105 reads

====================================================================================

graph has 2954 edges initially, 46 edges after simplification

total ucounts = 930
nonsolo ucounts = 432[2^187, 3^88, 4^58, 5^34, 6^22, 7^16, 8^3, 9^4, 10^6, 11^4, 12^2, 13^2, 14^2, 15, 20, 22, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.572 = ACCCACTGTCTGCCAG-1

using 208 reads

====================================================================================

graph has 102 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 2[3, 199]
surviving nonsolo ucounts = 1[199]
ids = [3]

====================================================================================

UMI info for barcode ACCCACTGTCTGCCAG-1 contig 1 = AGGAGGCAGC...
umi CATTCTGGAT = 196 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=480]
0-30 ==> 11-41 on |338|IGLV2-14|5'UTR| [len=41] (mis=0)
30-383 ==> 0-353 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=0)
383-421 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=3)
421-480 ==> 0-59 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 24 reads
cdr3 = CSSYTSSSTLWVF at 354, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 195
start codons at 30, 187, 231, 238
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.575 = ACCCACTGTGAGTATA-1

using 87 reads

====================================================================================

graph has 38 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 3[3^2, 74]
surviving nonsolo ucounts = 1[74]
ids = [2]

====================================================================================

UMI info for barcode ACCCACTGTGAGTATA-1 contig 1 = AGTCCCACTC...
umi CCATACCGGG = 74 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=499]
0-20 ==> 7-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
20-371 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
370-408 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
408-499 ==> 0-91 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 12 reads
cdr3 = CLQYSSSPWTF at 347, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 72
start codons at 20, 26, 95, 231, 450
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.578 = ACCCACTGTGTCGCTG-1

using 264 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 6[2^2, 3, 6^2, 241]
surviving nonsolo ucounts = 1[241]
ids = [9]

====================================================================================

UMI info for barcode ACCCACTGTGTCGCTG-1 contig 1 = AGCTTCAGCT...
umi TTTTAGACCC = 239 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=601]
0-47 ==> 67-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
47-355 ==> 0-308 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=14)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-601 ==> 0-166 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CVAWDDSLYAWVF at 368, score = 8 + 7
umis assigned: [9]
of which 1 are surviving nonsolos
reads assigned: 237
start codons at 47, 351, 381, 393
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.585 = ACCCACTGTTGAGTTC-1

using 317 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[315]
surviving nonsolo ucounts = 1[315]
ids = [2]

====================================================================================

UMI info for barcode ACCCACTGTTGAGTTC-1 contig 1 = AGGAGTCAGT...
umi GGACTCTCGG = 303 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=511]
0-27 ==> 4-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
27-378 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
379-418 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
418-511 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 47 reads
cdr3 = CQQYDNLPRYTF at 354, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 296
start codons at 27, 33, 89, 102, 241, 364, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.597 = ACCCACTTCAGTCAGT-1

using 379 reads

====================================================================================

graph has 176 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[2, 370]
surviving nonsolo ucounts = 1[370]
ids = [2]

====================================================================================

UMI info for barcode ACCCACTTCAGTCAGT-1 contig 1 = TGGGAGTCAG...
umi ATAACCCCAC = 359 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=488]
34-282 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
378-416 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
416-488 ==> 0-72 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CLHYDNRRRTF at 355, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 354
start codons at 34, 90, 103, 242, 365, 458
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.599 = ACCCACTTCATCTGCC-1

using 456 reads

====================================================================================

graph has 116 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^2, 137, 310]
surviving nonsolo ucounts = 2[137, 310]
ids = [1, 3]

====================================================================================

UMI info for barcode ACCCACTTCATCTGCC-1 contig 1 = GAAGAGCTGC...
umi CTCATTATGG = 303 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=494]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-369 ==> 0-336 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=17)
378-415 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
415-494 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 42 reads
cdr3 = CQQYRNSITF at 357, score = 9 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 303
start codons at 33, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.610 = ACCCACTTCCTTTCTC-1

using 13 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[13]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.629 = ACCCACTTCTCTGTCG-1

using 76 reads

====================================================================================

graph has 95 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 10[2, 3, 4, 5, 6, 7, 8, 9, 11, 17]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.635 = ACCCACTTCTTCGGTC-1

using 139 reads

====================================================================================

graph has 58 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[138]
surviving nonsolo ucounts = 1[138]
ids = [1]

====================================================================================

UMI info for barcode ACCCACTTCTTCGGTC-1 contig 1 = AAAAACCACA...
umi GTTGAGTCTG = 138 reads: +436 validated

GOOD CONTIGS

TIG 1[bases=486]
0-50 ==> 9-59 on |64|IGHV1-18|5'UTR| [len=59] (mis=1)
50-403 ==> 0-353 on |65|IGHV1-18|L-REGION+V-REGION| [len=353] (mis=22)
452-486 ==> 12-46 on |54|IGHJ4|J-REGION| [len=46] (mis=0)
junction support: 1 umis using 7 reads
cdr3 = CARDKHFRDPYYHYSGALDHW at 392, score = 7 + 7
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 136
start codons at 50, 248, 253, 270, 314, 347
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.642 = ACCGTAAAGAAGGCCT-1

using 553 reads

====================================================================================

graph has 170 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 3[2^2, 548]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================

REJECT CONTIGS

TIG 1[bases=445]
1-113 ==> 2195-2307 on rc of segment after IGKJ1 exon 1 [len=6000] (mis=0)
109-271 ==> 5838-6000 on rc of segment after IGKJ1 exon 1 [len=6000] (mis=1)
271-309 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
309-445 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
umis assigned: [2]
of which 0 are surviving nonsolos
reads assigned: 540
start codons at 26, 79, 134, 139, 252, 351
confident = false
did not find CDR3
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.646 = ACCGTAAAGAATTGTG-1

using 33358 reads

====================================================================================

graph has 12445 edges initially, 90 edges after simplification

total ucounts = 1568
nonsolo ucounts = 850[2^240, 3^193, 4^121, 5^60, 6^47, 7^26, 8^19, 9^10, 10^5, 11^6, 12^4, 13, 14^2, 16, 17, 18, 22, 35, 36, 38, 49, 53, 56, 57, 85^2, 89, 98, 103, 108, 110, 117, 121, 137, 138, 148, 154, 160, 169, 170, 184, 187^2, 197, 199, 201, 205, 207^2, 213, 216, 218^2, 223, 225, 233, 235^2, 236, 242, 246, 248, 249, 258^2, 261, 263, 267, 268, 270, 273, 275^2, 277, 278^2, 280, 282, 286, 288^2, 291, 295, 298, 300, 302, 304^2, 312, 313, 318^2, 319, 322, 324, 325, 326, 329, 330, 333, 334, 335, 337, 338^2, 345, 346, 347, 348^2, 351^2, 352, 353^2, 355, 358, 360, 363, 372, 399, 401, 412, 432, 464, 549, 567, 660, 729]
surviving nonsolo ucounts = 104[53, 56, 85^2, 89, 98, 110, 117, 121, 137, 138, 148, 154, 160, 169, 170, 184, 187^2, 197, 199, 201, 205, 207^2, 213, 216, 218^2, 223, 225, 233, 235^2, 236, 242, 246, 248, 249, 258^2, 261, 263, 267, 268, 270, 273, 275^2, 277, 278^2, 280, 282, 286, 288^2, 291, 295, 298, 300, 302, 304^2, 312, 313, 318^2, 319, 322, 324, 325, 326, 329, 333, 334, 335, 337, 338^2, 345, 346, 347, 348^2, 351^2, 352, 353^2, 355, 358, 360, 363, 372, 399, 401, 412, 432, 464, 549, 567, 660, 729]
ids = [718, 1194, 1142, 1485, 1126, 638, 530, 288, 1544, 446, ...]

====================================================================================

UMI info for barcode ACCGTAAAGAATTGTG-1 contig 1 = TGGGGGAGGA...
umi AACATTGCCA = 357 reads: +382 validated
umi AACCAACTCT = 318 reads: +382 validated
umi AAGGTAGGTA = 367 reads: +382 validated
umi AATTTTCTCC = 353 reads: +382 validated
umi ACATACAGGG = 308 reads: +382 validated
umi ACCGTAGTAG = 337 reads: +382 validated
umi ACCTATGCCA = 269 reads: +382 validated
umi ACGATTAAGG = 270 reads: +382 validated
umi AGAAACTATG = 289 reads: +382 validated
umi AGAACTGATA = 315 reads: +382 validated
umi AGAAGGATCG = 462 reads: +382 validated
umi AGATCCTGAA = 346 reads: +382 validated
umi AGCCCTTGGT = 332 reads: +382 validated
umi AGCCGATTAT = 208 reads: +382 validated
umi ATGGTTAAGG = 285 reads: +382 validated
umi ATTGCATGAG = 187 reads: +382 validated
umi CAATTACGAC = 332 reads: +382 validated
umi CAATTTAACA = 352 reads: +382 validated
umi CACCCGGCAT = 355 reads: +382 validated
umi CACCCTAGCT = 292 reads: +382 validated
umi CACTGAGCCG = 378 reads: +382 validated
umi CACTGTTTAC = 353 reads: +382 validated
umi CCAATCGTTA = 228 reads: +382 validated
umi CCAATGATTA = 551 reads: +382 validated
umi CCAGGGCCTG = 236 reads: +382 validated
umi CCGAACTTCG = 272 reads: +382 validated
umi CCGAAGGGCC = 325 reads: +382 validated
umi CCGGCGCATC = 329 reads: +382 validated
umi CCGTCAATTG = 305 reads: +382 validated
umi CCTAAAGCCA = 280 reads: +382 validated
umi CCTCAATAAC = 327 reads: +382 validated
umi CCTTAGATAT = 276 reads: +382 validated
umi CGATATCTGG = 151 reads: +382 validated
umi CGCCTACTGG = 349 reads: +382 validated
umi CGGGTCGGGT = 302 reads: +382 validated
umi CGTTGTTCTA = 352 reads: +382 validated
umi CTAGCTTGGC = 217 reads: +382 validated
umi CTGCTCGCTC = 336 reads: +382 validated
umi CTTAAAACTG = 258 reads: +382 validated
umi CTTGCGTGTA = 354 reads: +382 validated
umi GATCTCCGTA = 362 reads: +382 validated
umi GATTGGGGAA = 202 reads: +382 validated
umi GCCAGTATCA = 274 reads: +382 validated
umi GCGCCGATAG = 218 reads: +382 validated
umi GGATTTAGGC = 320 reads: +382 validated
umi GGGACAACGG = 183 reads: +382 validated
umi GGTCACATAT = 400 reads: +382 validated
umi GTCACGGGGG = 349 reads: +382 validated
umi GTTTCTCCTG = 241 reads: +382 validated
umi TAAGTATAGC = 317 reads: +382 validated
umi TACATAATAC = 160 reads: +83 -1XX +12 -1XX +7 -1XX +9 -1XX +4 -60 +4 -1XX +9 -2XX +13 -1XX +5 -1XX +27 -1XX +15 -2XX +5 -1XX +1 -1XX +10 -69X +12 -1XX +8 -1XX +10 -1XX +2 invalidated
umi TACCATCTTT = 201 reads: +382 validated
umi TACTGATACC = 339 reads: +382 validated
umi TAGGCTCTAG = 264 reads: +382 validated
umi TAGTGTCCTT = 325 reads: +382 validated
umi TATGATACGC = 271 reads: +382 validated
umi TATTAGGATT = 266 reads: +382 validated
umi TCAAGGGATA = 316 reads: +382 validated
umi TCACCATGGC = 314 reads: +382 validated
umi TCCACAGTCT = 571 reads: +382 validated
umi TCCGTTTGTG = 665 reads: +382 validated
umi TCGAGTTGTT = 204 reads: +382 validated
umi TCGCTCATTG = 401 reads: +382 validated
umi TCTCGCCTGT = 286 reads: +382 validated
umi TGACACCCGG = 279 reads: +382 validated
umi TGCCTCCATC = 337 reads: +382 validated
umi TGCTGGGGGG = 407 reads: +382 validated
umi TGGTATTGGG = 298 reads: +382 validated
umi TGTATCTGCA = 237 reads: +228 -1X +3 -2XX +148 invalidated
umi TTCCATTACG = 282 reads: +382 validated
umi TTGCGCTTCG = 279 reads: +382 validated
umi TTGTGTGGCC = 264 reads: +382 validated
umi TTTCGTTAAG = 296 reads: +382 validated

UMI info for barcode ACCGTAAAGAATTGTG-1 contig 2 = AGCCCTCAGA...
umi AATAAGGCGC = 195 reads: +430 validated
umi AATGCATTTA = 222 reads: +201 -1XX +228 invalidated
umi ATATAGAATG = 113 reads: +430 validated
umi ATCCATCTTT = 221 reads: +201 -1XX +228 invalidated
umi ATTATTGCCT = 250 reads: +86 -1XX +334 -1 +2 -1 +2 -2 +1 invalidated
umi CACGTAGCTT = 251 reads: +201 -1XX +228 invalidated
umi CAGCCTAGAG = 238 reads: +430 validated
umi CAGGGCCCCA = 138 reads: +430 validated
umi CCAGAGTCAA = 187 reads: +430 validated
umi CCCCGTGGCG = 111 reads: +430 validated
umi CGCGGGGTCA = 102 reads: +201 -1XX +214 -1 +2 -1 +2 -1 +7 invalidated
umi CGTCGCCTCC = 158 reads: +430 validated
umi CTCCCCATGA = 56 reads: +201 -1XX +228 invalidated
umi CTGATTGGAC = 259 reads: +201 -1XX +228 invalidated
umi CTTCTATATC = 201 reads: +430 validated
umi CTTGGCCGTA = 272 reads: +201 -1XX +228 invalidated
umi GGATATTACC = 168 reads: +430 validated
umi TAAAATATCG = 218 reads: +394 -2 +12 -1 +1 -2 +18 non-validated
umi TAAACTGTCA = 85 reads: +430 validated
umi TAATCTACGT = 91 reads: +201 -1XX +228 invalidated
umi TAGCAAATAT = 170 reads: +430 validated
umi TAGCACGGTT = 57 reads: +398 -1 +31 non-validated
umi TATGATCTGC = 262 reads: +201 -1XX +228 invalidated
umi TCCCATGCTG = 733 reads: -290X +140 invalidated
umi TCCGCGTATG = 147 reads: +201 -1XX +228 invalidated
umi TTCCCCATGA = 89 reads: +201 -1XX +228 invalidated
umi TTGTAGCCTC = 125 reads: -390X +3 -2XX +7 -1XX +1 -1XX +2 -1XX +3 -2XX +8 -1XX +8 invalidated
umi TTTCTATGAG = 127 reads: +201 -1XX +228 invalidated

GOOD CONTIGS

TIG 1[bases=557]
39-287 ==> 0-248 on |237|IGKV1-8|L-REGION+V-REGION| [len=345] (mis=34)
383-421 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
421-557 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 72 umis using 3417 reads
cdr3 = CLHYDNRRRTF at 360, score = 9 + 8
umis assigned: [18, 19, 50, 96, 115, 140, 141, 159, 199, 204] and 63 others
of which 72 are surviving nonsolos
reads assigned: 22241
start codons at 39, 95, 108, 247, 370, 463
confident = true

TIG 2[bases=669]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=2)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=33)
463-509 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=4)
509-669 ==> 0-160 on |1|IGHA1|C-REGION| [len=1058] (mis=0)
junction support: 24 umis using 398 reads
cdr3 = CARESYVGLYSSTSYPDYW at 421, score = 8 + 7
umis assigned: [64, 80, 288, 305, 356, 422, 437, 446, 501, 530] and 18 others
of which 28 are surviving nonsolos
reads assigned: 5079
start codons at 79, 228, 235, 314, 356, 382, 437, 563
confident = true
now this is a cell
paired!

GCTGTGTATTACTGTGCGAGAGAATCTTATGTCGGGCTCTATTCTTCAACCAGTTATCCCGACTACTGGGGCCAGGGAACCCTGGTCACCGTCTCCTCAG <==> GTCTCCAGCCTGCAACCTGAAGATTTTGCCACCTATTACTGTCTACACTATGATAATCGCCGTCGCACCTTCGGCCAAGGGACACGACTGGAGATTAAAC

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.648 = ACCGTAAAGACAGGCT-1

using 1023 reads

====================================================================================

graph has 1480 edges initially, 10 edges after simplification

total ucounts = 496
nonsolo ucounts = 185[2^66, 3^40, 4^29, 5^22, 6^12, 7^4, 8^4, 9, 11, 12, 13^2, 14, 15^2]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 5.650 = ACCGTAAAGAGCCCAA-1

using 335 reads

====================================================================================

graph has 164 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 1[333]
surviving nonsolo ucounts = 1[333]
ids = [2]

====================================================================================

UMI info for barcode ACCGTAAAGAGCCCAA-1 contig 1 = GAGTCAGACT...
umi GGGTACTCGC = 339 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=546]
0-25 ==> 156-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
25-376 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=25)
373-410 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=5)
410-546 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 51 reads
cdr3 = CHQYNSYSTF at 352, score = 8 + 7
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 327
start codons at 25, 31, 87, 100, 236, 239, 332, 452
confident = false
NOT paired!

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BARCODE 5.657 = ACCGTAAAGCACGCCT-1

using 1127 reads

====================================================================================

graph has 1594 edges initially, 12 edges after simplification

total ucounts = 589
nonsolo ucounts = 220[2^102, 3^44, 4^27, 5^16, 6^12, 7^8, 8^5, 9^2, 10^2, 13, 15]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!
sorting bam, mem = 0.12
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk005-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk005-ucc6a7a72ce/files/contig_bam.bam

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59.518 seconds used processing barcodes, peak mem = 0.29
