[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.38 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk002-ucc6a7a72ce /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk002-ucc6a7a72ce/files /scratch/etanis/ST-118/multi/dimitri-multi/journal/SC_MULTI_CS.SC_MULTI_CORE.MULTI_GEM_WELL_PROCESSOR.VDJ_B_GEM_WELL_PROCESSOR.SC_VDJ_CONTIG_ASSEMBLER.ASSEMBLE_VDJ.fork0.chnk002.ucc6a7a72ce
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.0 = AACTGGTGTTATCGGT-1

using 31326 reads

====================================================================================

graph has 7846 edges initially, 42 edges after simplification

total ucounts = 910
nonsolo ucounts = 485[2^142, 3^100, 4^45, 5^26, 6^23, 7^8, 8^12, 9, 10^4, 11^2, 12, 13, 14, 18, 20, 21, 22, 29, 37, 103, 118, 126, 129, 130, 132, 133, 134, 138, 143, 153, 155, 158, 165^2, 170, 184, 189, 192, 194^2, 206, 212, 213, 219, 221, 222, 226, 230, 232, 236, 237, 240^2, 246, 247, 250, 252, 254^2, 258, 259, 260^2, 262, 266^2, 267, 268^3, 269^2, 270^2, 272, 273, 274, 276, 277^2, 278, 279^2, 281, 282, 284^2, 285^2, 286, 288^2, 290, 291^2, 293, 296^3, 299^3, 300, 301, 303, 305, 306^2, 308, 312, 313, 316^2, 321^2, 322, 323^2, 328, 329, 334, 337, 340^2, 341, 342, 345, 348, 355, 363, 368, 374]
surviving nonsolo ucounts = 110[129, 130, 132, 133, 134, 138, 143, 153, 155, 158, 165^2, 170, 184, 189, 192, 194^2, 206, 212, 213, 219, 221, 222, 226, 230, 232, 236, 237, 240^2, 246, 247, 250, 252, 254^2, 258, 259, 260^2, 262, 266^2, 267, 268^3, 269^2, 270^2, 272, 273, 274, 276, 277^2, 278, 279^2, 281, 282, 284^2, 285^2, 286, 288^2, 290, 291^2, 293, 296^3, 299^3, 300, 301, 303, 305, 306^2, 308, 312, 313, 316^2, 321^2, 322, 323^2, 328, 329, 334, 337, 340^2, 341, 342, 345, 348, 355, 363, 368, 374]
ids = [461, 827, 435, 429, 894, 453, 889, 69, 40, 486, ...]

====================================================================================

UMI info for barcode AACTGGTGTTATCGGT-1 contig 1 = GGGGAGGAGT...
umi AAAAACGGCA = 314 reads: +388 validated
umi AAAATACTTA = 350 reads: +388 validated
umi AAAGAGCGTG = 305 reads: +388 validated
umi AAAGCAGGTC = 337 reads: +388 validated
umi AAAGGCACAC = 298 reads: +388 validated
umi AAGTTACACC = 340 reads: +388 validated
umi AATCATCTTG = 289 reads: +388 validated
umi AATCGAGGTG = 305 reads: +388 validated
umi AATCGTGCCG = 155 reads: +388 validated
umi ACACGCTCCG = 327 reads: +388 validated
umi ACATAGAAAG = 307 reads: +388 validated
umi ACCGTCGTAG = 154 reads: +388 validated
umi ACGACTATGA = 218 reads: +388 validated
umi ACGGCGTCAA = 245 reads: +388 validated
umi ACGGCGTTGG = 278 reads: +388 validated
umi ACTTCTAATG = 166 reads: +388 validated
umi ACTTGACCAT = 237 reads: +388 validated
umi AGATATTAGG = 287 reads: +388 validated
umi AGGTGATTCG = 284 reads: +388 validated
umi ATCAGAATTA = 341 reads: +388 validated
umi ATCAGACCTT = 313 reads: +142 -1XX +245 invalidated
umi ATCATACTCA = 274 reads: +388 validated
umi ATCGTTATAT = 266 reads: +388 validated
umi ATGAATTACT = 274 reads: +388 validated
umi ATGGAGTGGT = 337 reads: +388 validated
umi ATTGCATGAG = 264 reads: +388 validated
umi ATTTGCACAC = 296 reads: +388 validated
umi CAAATGAACC = 254 reads: +388 validated
umi CAAGGCGTAC = 323 reads: +388 validated
umi CAATGCTTTT = 280 reads: +388 validated
umi CAGTGCCTCC = 285 reads: +388 validated
umi CATACGCCGG = 290 reads: +388 validated
umi CATATGTCGG = 238 reads: +388 validated
umi CATTCTCTTC = 306 reads: +388 validated
umi CCAGGGCCCG = 219 reads: +388 validated
umi CCAGGTGCAG = 267 reads: +388 validated
umi CCCGAGACCT = 279 reads: +388 validated
umi CCCGGCGCGC = 275 reads: +388 validated
umi CCCGTGTCCA = 319 reads: +388 validated
umi CCGCCTTAAC = 281 reads: +388 validated
umi CCTGGGCAGT = 313 reads: +388 validated
umi CCTTCTTATA = 268 reads: +388 validated
umi CGACCTTTTG = 349 reads: +388 validated
umi CGCAGCGGTT = 293 reads: +388 validated
umi CGGAGGTGGG = 258 reads: +388 validated
umi CGGTTACCAC = 328 reads: +388 validated
umi CGGTTGTGGC = 275 reads: +388 validated
umi CGTTTTAGCG = 295 reads: +388 validated
umi CGTTTTTGTG = 193 reads: +388 validated
umi CTATTTCTGC = 318 reads: +388 validated
umi CTCCCATGCC = 131 reads: +388 validated
umi CTCGCTTGGT = 134 reads: +388 validated
umi CTTAATGCCT = 137 reads: +388 validated
umi CTTACGTCAA = 265 reads: +388 validated
umi CTTGTGGGTT = 298 reads: +388 validated
umi CTTTGGATAC = 333 reads: +388 validated
umi GAACAGCAGG = 368 reads: +388 validated
umi GAATCTAACC = 155 reads: +388 validated
umi GAGCCAGATA = 355 reads: +388 validated
umi GCAATGAGTA = 252 reads: +388 validated
umi GCTACCTCTT = 214 reads: +388 validated
umi GCTGGTGGGG = 297 reads: +388 validated
umi GCTGTGTTGG = 334 reads: +388 validated
umi GGACTTAATG = 221 reads: +388 validated
umi GGAGCATCTC = 205 reads: +388 validated
umi GGGAGATGCA = 242 reads: +388 validated
umi GGGGCGGCCA = 264 reads: +388 validated
umi GTAAGCATCG = 276 reads: +388 validated
umi GTATATCCAG = 291 reads: +388 validated
umi GTTTTCCCAC = 277 reads: +388 validated
umi TACAAAATGC = 232 reads: +388 validated
umi TACAGGATCT = 240 reads: +388 validated
umi TACCATGCCA = 283 reads: +388 validated
umi TACGTCTGGG = 283 reads: +388 validated
umi TAGTCGGGAG = 291 reads: +388 validated
umi TATACTTGTA = 312 reads: +388 validated
umi TATGCTCTGC = 227 reads: +388 validated
umi TCAAGCTCAG = 215 reads: +388 validated
umi TCAATGCGGA = 260 reads: +388 validated
umi TCACGCGGTC = 187 reads: +388 validated
umi TCCTCAGGTC = 194 reads: +388 validated
umi TCTAACCATG = 329 reads: +388 validated
umi TGACATTCCC = 244 reads: +388 validated
umi TGACTATGCG = 260 reads: +388 validated
umi TGCCGGGACG = 271 reads: +388 validated
umi TGGTGCCAAT = 365 reads: +388 validated
umi TGTCACGTAT = 297 reads: +388 validated
umi TGTGGGTCTC = 378 reads: +388 validated
umi TGTGTCACCC = 275 reads: +388 validated
umi TTAGATTCTG = 270 reads: +388 validated
umi TTTACCCTTT = 188 reads: +388 validated
umi TTTATAATCA = 269 reads: +388 validated
umi TTTATAGCCT = 142 reads: +388 validated
umi TTTCATATCG = 133 reads: +388 validated
umi TTTGCGGGCT = 171 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=555]
0-31 ==> 0-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=2)
31-382 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
382-419 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 95 umis using 3850 reads
cdr3 = CQQYDNLLLTF at 358, score = 9 + 9
umis assigned: [0, 3, 9, 10, 11, 31, 37, 39, 40, 55] and 85 others
of which 95 are surviving nonsolos
reads assigned: 25045
start codons at 31, 37, 93, 106, 245, 368, 461
confident = true

REJECT CONTIGS

TIG 1[bases=606]
0-90 ==> 3254-3344 on rc of segment before IGKV2-24 exon 2 [len=3774] (mis=6)
0-90 ==> 3251-3341 on segment before IGKV2D-23 exon 1 [len=3772] (mis=6)
30-78 ==> 0-48 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=0)
90-119 ==> 3343-3372 on rc of segment before IGKV2-24 exon 2 [len=3774] (mis=0)
90-119 ==> 3340-3369 on segment before IGKV2D-23 exon 1 [len=3772] (mis=0)
120-281 ==> 48-209 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=0)
282-433 ==> 209-360 on |271|IGKV2D-26|L-REGION+V-REGION| [len=360] (mis=1) [SHIFT!]
431-470 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
470-606 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
cdr3 = CMQDAQDPYTF at 409, score = 9 + 8
umis assigned: [48, 80, 153, 216, 218, 245, 253, 461, 504, 546] and 5 others
of which 15 are surviving nonsolos
reads assigned: 3692
start codons at 30, 63, 95, 108, 141, 192, 229, 293, 392, 412, 419, 512
confident = false
not full
frameshifted full length stopped transcript of length 606
VJ delta = -28
delta too large
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.3 = AACTGGTGTTCCGTCT-1

using 839 reads

====================================================================================

graph has 224 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 2[3, 836]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.13 = AACTGGTTCAAGCCTA-1

using 259 reads

====================================================================================

graph has 82 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 3[2, 9, 244]
surviving nonsolo ucounts = 1[244]
ids = [0]

====================================================================================

UMI info for barcode AACTGGTTCAAGCCTA-1 contig 1 = GGGGAAGCTC...
umi ATACACCGGG = 246 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=552]
0-56 ==> 0-56 on |326|IGLV1-47|5'UTR| [len=56] (mis=0)
56-407 ==> 0-351 on |327|IGLV1-47|L-REGION+V-REGION| [len=351] (mis=1)
409-447 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=0)
447-552 ==> 0-105 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 29 reads
cdr3 = CAAWDDSLSGSYVF at 377, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 243
start codons at 56, 210, 360, 385, 390, 411
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.17 = AACTGGTTCACGGTTA-1

using 12 reads

====================================================================================

graph has 4 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[12]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.18 = AACTGGTTCAGCAACT-1

using 292 reads

====================================================================================

graph has 90 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[292]
surviving nonsolo ucounts = 1[292]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=565]
39-160 ==> 0-121 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=18)
160-303 ==> 123-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=39) [SHIFT!]
215-390 ==> 196-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=31) [SHIFT!]
424-470 ==> 0-46 on |54|IGHJ4|J-REGION| [len=46] (mis=12)
470-565 ==> 0-95 on |45|IGHG3|C-REGION| [len=1130] (mis=0)
cdr3 = CARGPLVRGILGKGYYLDLW at 379, score = 8 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 284
start codons at 39, 83, 349
confident = false
frameshifted full length stopped transcript of length 565
VJ delta = 8
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.35 = AACTGGTTCGGCGCAT-1

using 244 reads

====================================================================================

graph has 84 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[3, 238]
surviving nonsolo ucounts = 1[238]
ids = [0]

====================================================================================

UMI info for barcode AACTGGTTCGGCGCAT-1 contig 1 = GGGAGGAACT...
umi AGCCTAAAAT = 225 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=459]
0-35 ==> 12-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
35-380 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=3)
380-417 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
417-459 ==> 0-42 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CQQRSNWPLTF at 356, score = 9 + 9
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 222
start codons at 35, 243
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.40 = AACTGGTTCGTAGGAG-1

using 284 reads

====================================================================================

graph has 154 edges initially, 2 edges after simplification

total ucounts = 18
nonsolo ucounts = 12[2^3, 4, 5^4, 6^2, 9, 227]
surviving nonsolo ucounts = 1[227]
ids = [7]

====================================================================================

UMI info for barcode AACTGGTTCGTAGGAG-1 contig 1 = AGTGCTTTCT...
umi CTTATGGCGT = 206 reads: +457 validated

GOOD CONTIGS

TIG 1[bases=483]
17-388 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=0)
400-427 ==> 0-27 on |22|IGHD3-9|D-REGION| [len=27] (mis=4)
426-474 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
junction support: 1 umis using 12 reads
cdr3 = CARGQRFSYYDILTGFRFFDYW at 377, score = 9 + 7
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 203
start codons at 17, 38, 82, 168
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.56 = AACTTTCAGACAAAGG-1

using 1459 reads

====================================================================================

graph has 2147 edges initially, 22 edges after simplification

total ucounts = 625
nonsolo ucounts = 284[2^106, 3^62, 4^29, 5^35, 6^16, 7^13, 8^6, 9^4, 10^5, 11, 12^2, 13^3, 14, 20]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.59 = AACTTTCAGACGCAAC-1

using 589 reads

====================================================================================

graph has 252 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 5[2, 3, 8, 275, 297]
surviving nonsolo ucounts = 2[275, 297]
ids = [1, 3]

====================================================================================

UMI info for barcode AACTTTCAGACGCAAC-1 contig 1 = ACATGGGAAG...
umi CCGGTAGGCT = 264 reads: +436 validated

UMI info for barcode AACTTTCAGACGCAAC-1 contig 2 = GAAGAGCTGC...
umi AGTGAGTTCC = 259 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=503]
0-25 ==> 6-31 on |185|IGHV4-34|5'UTR| [len=31] (mis=0)
25-396 ==> 0-371 on |186|IGHV4-34|L-REGION+V-REGION| [len=371] (mis=7)
413-461 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=5)
461-503 ==> 0-42 on |6|IGHD|C-REGION| [len=1289] (mis=0)
junction support: 1 umis using 21 reads
cdr3 = CARVWASDVWYLAYW at 385, score = 9 + 7
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 260
start codons at 2, 25, 46, 90, 176, 407, 479
confident = false

TIG 2[bases=514]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=26)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
421-514 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQYGSSPMYTF at 357, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 254
start codons at 33, 82, 241, 340, 381, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.62 = AACTTTCAGATATGCA-1

using 289 reads

====================================================================================

graph has 105 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 1[282]
surviving nonsolo ucounts = 1[282]
ids = [4]

====================================================================================

UMI info for barcode AACTTTCAGATATGCA-1 contig 1 = AGTCAGTCCC...
umi CCCACTGCAT = 280 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=545]
0-24 ==> 7-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
24-375 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=2)
371-409 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=3)
409-545 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 58 reads
cdr3 = CQQYDNLPTF at 351, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 278
start codons at 24, 30, 86, 99, 238, 361, 451
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.64 = AACTTTCAGCACACAG-1

using 362 reads

====================================================================================

graph has 592 edges initially, 2 edges after simplification

total ucounts = 190
nonsolo ucounts = 78[2^40, 3^17, 4^8, 5^3, 6^4, 7^4, 9, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.70 = AACTTTCAGCGTTGCC-1

using 311 reads

====================================================================================

graph has 92 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 1[307]
surviving nonsolo ucounts = 1[307]
ids = [4]

====================================================================================

UMI info for barcode AACTTTCAGCGTTGCC-1 contig 1 = AGCTCTGGGA...
umi TTTTATCCAT = 290 reads: +427 validated

GOOD CONTIGS

TIG 1[bases=529]
0-80 ==> 0-80 on |139|IGHV3-43|5'UTR| [len=80] (mis=3)
80-435 ==> 0-355 on |140|IGHV3-43|L-REGION+V-REGION| [len=355] (mis=27)
459-507 ==> 0-48 on |55|IGHJ4|J-REGION| [len=48] (mis=4)
507-529 ==> 0-22 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 13 reads
cdr3 = CAKDAVLRGAVADKIDYW at 422, score = 9 + 7
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 287
start codons at 80, 225, 231, 236, 315, 383, 432
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.72 = AACTTTCAGCTGCAAG-1

using 450 reads

====================================================================================

graph has 214 edges initially, 2 edges after simplification

total ucounts = 11
nonsolo ucounts = 4[3, 5, 15, 420]
surviving nonsolo ucounts = 1[420]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCAGCTGCAAG-1 contig 1 = GGAGCCCCAG...
umi AAACCCCGTT = 391 reads: +409 validated

GOOD CONTIGS

TIG 1[bases=508]
0-68 ==> 168-236 on |170|IGHV3-74|5'UTR| [len=236] (mis=1)
68-421 ==> 0-353 on |171|IGHV3-74|L-REGION+V-REGION| [len=353] (mis=13)
428-477 ==> 14-63 on |737|IGHJ6|J-REGION| [len=63] (mis=0)
477-508 ==> 0-31 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CARGNYYGMDVW at 410, score = 9 + 7
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 381
start codons at 68, 224, 285, 371, 434
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.73 = AACTTTCAGGACTGGT-1

using 224 reads

====================================================================================

graph has 64 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[224]
surviving nonsolo ucounts = 1[224]
ids = [0]

====================================================================================

REJECT CONTIGS

TIG 1[bases=467]
41-178 ==> 0-137 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=25)
178-304 ==> 140-266 on |197|IGHV4/OR15-8|L-REGION+V-REGION| [len=353] (mis=30)
cdr3 = CARGPLVRGILGKGYYLDLW at 380, score = 8 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 215
start codons at 41, 85, 350
confident = false
see deletion of 3 bases at pos 137 on |197|IGHV4/OR15-8|L-REGION+V-REGION|
>vscore_2.73_77.1%
ATGAAACACCTGTGGTTCTTCCTCCTCCTGGTGGCAGCTCCCAGATGCGTCCTGTCGCAGGGACAGCTGCGAGAGTGGGGCGGAGGCCTGTTGGAGACCTCGCAGACGCTGTCACTCACCTGTTCTGTTTACGGTCGAGTCTTCGCTGGCTCCTTCTGGACTTGGATCCGCCAATCCCCAGGCAAGGGGCCGGAGTGGATTGGAGAAATTGCTCAGGGTGGAAGACCCAACTACAACCCGGCCCTCCAGAGTCGCGTCACCATTTCAACTGACACGTCGCAGATCCAGTTCTCCCTGACTGTGAGGTCTATGACCGCCGCGGACTCGGCTACATATTACTGTGCCAGGGG
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.78 = AACTTTCAGGTAAACT-1

using 577 reads

====================================================================================

graph has 210 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[11, 193, 368]
surviving nonsolo ucounts = 2[193, 368]
ids = [2, 3]

====================================================================================

UMI info for barcode AACTTTCAGGTAAACT-1 contig 1 = GGAGTCAGTC...
umi ATATAGCACC = 194 reads: +388 validated
umi ATTAGGACTG = 367 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
26-379 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=9)
377-414 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 2 umis using 97 reads
cdr3 = CQQSYTTLLTF at 353, score = 9 + 9
umis assigned: [2, 3]
of which 2 are surviving nonsolos
reads assigned: 551
start codons at 26, 32, 88, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.84 = AACTTTCAGTTTGCGT-1

using 382 reads

====================================================================================

graph has 120 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 39, 339]
surviving nonsolo ucounts = 2[39, 339]
ids = [3, 2]

====================================================================================

UMI info for barcode AACTTTCAGTTTGCGT-1 contig 1 = AATCAGTCCC...
umi GCTCCGATGG = 338 reads: +388 validated
umi TGCAGCGGAT = 37 reads: -388 non-validated

GOOD CONTIGS

TIG 1[bases=548]
0-24 ==> 3-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
24-375 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
374-412 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
412-548 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 60 reads
cdr3 = CLQYSSSPWTF at 351, score = 9 + 8
umis assigned: [2, 3]
of which 2 are surviving nonsolos
reads assigned: 373
start codons at 24, 30, 99, 235, 454
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.90 = AACTTTCCAAGACACG-1

using 2414 reads

====================================================================================

graph has 696 edges initially, 30 edges after simplification

total ucounts = 13
nonsolo ucounts = 7[3, 4, 162, 365, 386, 436, 1052]
surviving nonsolo ucounts = 5[162, 365, 386, 436, 1052]
ids = [1, 6, 3, 0, 8]

====================================================================================

UMI info for barcode AACTTTCCAAGACACG-1 contig 1 = GGGGAGGAAT...
umi CCTGCATAAC = 368 reads: +388 validated

UMI info for barcode AACTTTCCAAGACACG-1 contig 2 = GTCAGTCTCA...
umi AGTGCAGGCA = 389 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=555]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-555 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 59 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 361
start codons at 31, 37, 106, 242, 461
confident = true

TIG 2[bases=547]
0-23 ==> 0-23 on |252|IGKV1D-39|5'UTR| [len=23] (mis=0)
23-376 ==> 0-353 on |253|IGKV1D-39|L-REGION+V-REGION| [len=353] (mis=29)
374-411 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=1)
411-547 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 57 reads
cdr3 = CQQGYSIPLTF at 350, score = 9 + 9
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 383
start codons at 23, 29, 85, 234, 288, 453
confident = true

REJECT CONTIGS

TIG 1[bases=520]
1-271 ==> 86-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=11)
271-309 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
309-520 ==> 0-211 on |305|IGLC1|C-REGION| [len=317] (mis=0)
cdr3 = CQSYDSSLSGLYVF at 239, score = 7 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 432
start codons at 69, 72, 123, 222, 249, 273, 441
confident = false
not full
VJ delta = 22
not full
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.96 = AACTTTCCACAGGCCT-1

using 526 reads

====================================================================================

graph has 188 edges initially, 4 edges after simplification

total ucounts = 9
nonsolo ucounts = 3[5, 180, 335]
surviving nonsolo ucounts = 2[180, 335]
ids = [0, 2]

====================================================================================

UMI info for barcode AACTTTCCACAGGCCT-1 contig 1 = GAGGAACTGC...
umi CCGGGTAAAC = 318 reads: +382 validated

UMI info for barcode AACTTTCCACAGGCCT-1 contig 2 = TGGGTAGAGA...
umi AACTGATCAA = 181 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=3)
415-492 ==> 0-77 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 43 reads
cdr3 = CQQRSNWPLTF at 354, score = 9 + 9
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 312
start codons at 33, 238, 241, 457
confident = false

TIG 2[bases=546]
0-36 ==> 78-114 on |322|IGLV1-44|5'UTR| [len=114] (mis=0)
36-389 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=18)
386-424 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=1)
424-546 ==> 0-122 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 27 reads
cdr3 = CAAWDASLIGVVF at 357, score = 8 + 8
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 177
start codons at 36, 241, 322, 340, 365, 370
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.100 = AACTTTCCACCTCGTT-1

using 326 reads

====================================================================================

graph has 94 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[2, 321]
surviving nonsolo ucounts = 1[321]
ids = [0]

====================================================================================

UMI info for barcode AACTTTCCACCTCGTT-1 contig 1 = GGGGAGGAAC...
umi AGCGGATCGC = 310 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=471]
0-36 ==> 11-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
36-381 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=14)
380-418 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=7)
418-471 ==> 0-53 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CQQRRTWPPAF at 357, score = 9 + 6
umis assigned: [0]
of which 1 are surviving nonsolos
reads assigned: 305
start codons at 36, 85, 241, 460
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.103 = AACTTTCCACTGCCAG-1

using 392 reads

====================================================================================

graph has 256 edges initially, 4 edges after simplification

total ucounts = 11
nonsolo ucounts = 6[2, 4, 11, 24^2, 322]
surviving nonsolo ucounts = 2[24, 322]
ids = [2, 6]

====================================================================================

UMI info for barcode AACTTTCCACTGCCAG-1 contig 1 = GAGTCAGTCT...
umi CTAATACCTG = 299 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=492]
0-25 ==> 22-47 on |230|IGKV1-39|5'UTR| [len=47] (mis=0)
25-376 ==> 0-351 on |231|IGKV1-39|L-REGION+V-REGION| [len=351] (mis=11)
375-413 ==> 0-38 on |217|IGKJ5|J-REGION| [len=38] (mis=0)
413-492 ==> 0-79 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 39 reads
cdr3 = CQQSYRRPITF at 352, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 289
start codons at 25, 31, 87, 100, 236, 335, 455
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.106 = AACTTTCCAGACAAGC-1

using 243 reads

====================================================================================

graph has 156 edges initially, 2 edges after simplification

total ucounts = 15
nonsolo ucounts = 12[3, 4, 5, 6^2, 7^2, 8, 9, 15^2, 155]
surviving nonsolo ucounts = 1[155]
ids = [2]

====================================================================================

UMI info for barcode AACTTTCCAGACAAGC-1 contig 1 = CAGTTAGGAC...
umi ATCAAATACG = 153 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=503]
0-22 ==> 30-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
22-370 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=21)
369-407 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=2)
407-503 ==> 0-96 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 19 reads
cdr3 = CQQYDRSPRTF at 346, score = 9 + 8
umis assigned: [2]
of which 1 are surviving nonsolos
reads assigned: 150
start codons at 22, 329, 356, 449
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.109 = AACTTTCCAGCCACCA-1

using 10 reads

====================================================================================

graph has 8 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[10]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.118 = AACTTTCCATACGCCG-1

using 3627 reads

====================================================================================

graph has 2007 edges initially, 36 edges after simplification

total ucounts = 419
nonsolo ucounts = 164[2^63, 3^33, 4^17, 5^22, 6^6, 7^5, 9^2, 19, 90, 95, 119, 151, 164, 165, 187, 195, 206, 216, 224, 238, 253, 276, 282]
surviving nonsolo ucounts = 15[90, 95, 119, 151, 164, 165, 187, 195, 206, 216, 224, 238, 253, 276, 282]
ids = [32, 29, 294, 396, 416, 119, 120, 323, 12, 263, ...]

====================================================================================

UMI info for barcode AACTTTCCATACGCCG-1 contig 1 = AGCTCTCAGA...
umi ACAGACTAAC = 93 reads: +421 validated
umi ACATCGCAAG = 91 reads: +394 -1X +26 invalidated
umi CATCAGTAAC = 147 reads: +421 validated
umi CATCGTTCTG = 187 reads: +407 -1 +11 -1 +1 non-validated
umi GGTATATCTC = 215 reads: +383 -1 +4 -2 +31 non-validated
umi TAAATCTGAC = 118 reads: +421 validated
umi TTCGAGAATG = 278 reads: +421 validated

UMI info for barcode AACTTTCCATACGCCG-1 contig 2 = GCTGTGCTGT...
umi AAGGGAACCC = 208 reads: +388 validated
umi CCGCTTCTCC = 223 reads: +388 validated
umi GACAAAGCTC = 236 reads: +388 validated
umi GACAGGAGCC = 281 reads: +388 validated
umi GTGTCGGAGC = 253 reads: +388 validated
umi TATTCTATGA = 191 reads: +388 validated
umi TTCACATGAG = 150 reads: +388 validated
umi TTTGTAGTTC = 161 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=571]
0-79 ==> 0-79 on |141|IGHV3-48|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |142|IGHV3-48|L-REGION+V-REGION| [len=353] (mis=1)
448-500 ==> 0-52 on |50|IGHJ2|J-REGION| [len=52] (mis=5)
500-571 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 6 umis using 73 reads
cdr3 = CARAYWIQLSPYFDLW at 421, score = 9 + 7
umis assigned: [29, 32, 119, 120, 263, 294, 397]
of which 7 are surviving nonsolos
reads assigned: 1116
start codons at 79, 230, 235, 382
confident = true

TIG 2[bases=563]
0-43 ==> 0-43 on |362|IGLV3-25|5'UTR| [len=43] (mis=0)
43-380 ==> 0-337 on |363|IGLV3-25|L-REGION+V-REGION| [len=337] (mis=0)
393-431 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=0)
431-563 ==> 0-132 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 8 umis using 226 reads
cdr3 = CQSADSSGTYQGVVF at 358, score = 8 + 8
umis assigned: [12, 150, 216, 217, 282, 323, 396, 416]
of which 8 are surviving nonsolos
reads assigned: 1680
start codons at 43, 104, 173, 191
confident = true
now this is a cell
paired!

GAGGACACGGCTGTTTATTACTGTGCGAGAGCTTACTGGATACAGCTATCGCCTTACTTCGATCTCTGGGGCCGTGGCACCCTGGTCACTGTCTCCTCAG <==> CAGGCAGAAGACGAGGCTGACTATTACTGTCAATCAGCAGACAGCAGTGGTACTTATCAGGGTGTGGTATTCGGCGGAGGGACCAAGCTGACCGTCCTAG

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 2.123 = AACTTTCCATGGAATA-1

using 1725 reads

====================================================================================

graph has 1262 edges initially, 12 edges after simplification

total ucounts = 251
nonsolo ucounts = 127[2^41, 3^37, 4^14, 5^7, 6^7, 7^5, 8^3, 9^5, 10^3, 13, 93, 239, 324, 472]
surviving nonsolo ucounts = 4[93, 239, 324, 472]
ids = [158, 45, 154, 146]

====================================================================================

UMI info for barcode AACTTTCCATGGAATA-1 contig 1 = AGCTCTGAGA...
umi ATTTTCATTC = 241 reads: +424 validated
umi GAAATTCTCT = 476 reads: +424 validated

UMI info for barcode AACTTTCCATGGAATA-1 contig 2 = AGGCTGGTCA...
umi GCACATCACC = 311 reads: +388 validated

UMI info for barcode AACTTTCCATGGAATA-1 contig 3 = TGGGGCTGGG...
umi GCCCTCTGCA = 86 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=574]
0-79 ==> 0-79 on |121|IGHV3-23|5'UTR| [len=79] (mis=0)
79-432 ==> 0-353 on |122|IGHV3-23|L-REGION+V-REGION| [len=353] (mis=1)
453-503 ==> 0-50 on |53|IGHJ3|J-REGION| [len=50] (mis=2)
503-574 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 2 umis using 34 reads
cdr3 = CAKDLRFLEWANAFDIW at 421, score = 9 + 8
umis assigned: [45, 146]
of which 2 are surviving nonsolos
reads assigned: 701
start codons at 79, 230, 235, 382, 455, 484
confident = true

TIG 2[bases=528]
0-47 ==> 134-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
47-398 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=3)
397-435 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
435-528 ==> 0-93 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 36 reads
cdr3 = CQQYNSYPWTF at 374, score = 8 + 8
umis assigned: [154]
of which 1 are surviving nonsolos
reads assigned: 308
start codons at 47, 53, 109, 122, 354, 477
confident = true

TIG 3[bases=474]
45-399 ==> 0-354 on |339|IGLV2-14|L-REGION+V-REGION| [len=361] (mis=9)
395-433 ==> 0-38 on |311|IGLJ2|J-REGION| [len=38] (mis=2)
433-474 ==> 0-41 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 17 reads
cdr3 = CSSYTSSGTLVF at 369, score = 8 + 9
umis assigned: [158]
of which 1 are surviving nonsolos
reads assigned: 86
start codons at 45, 202, 246, 253, 256
confident = true
now this is a cell
NOT paired!

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BARCODE 2.128 = AACTTTCCATTCCTCG-1

using 22 reads

====================================================================================

graph has 24 edges initially, 2 edges after simplification

total ucounts = 3
nonsolo ucounts = 2[10, 11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

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BARCODE 2.136 = AACTTTCGTACCGTAT-1

using 329 reads

====================================================================================

graph has 124 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 6[2, 3, 6, 8, 15, 292]
surviving nonsolo ucounts = 1[292]
ids = [6]

====================================================================================

UMI info for barcode AACTTTCGTACCGTAT-1 contig 1 = GTCAGACTCA...
umi TGGTCTATCA = 288 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=501]
0-23 ==> 158-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=1)
23-374 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=7)
372-411 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=3)
411-501 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 44 reads
cdr3 = CQQYNSYPYTF at 350, score = 8 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 283
start codons at 23, 29, 85, 98, 234, 237, 330, 453
confident = false
NOT paired!
sorting bam, mem = 0.09
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk002-ucc6a7a72ce/files/contig_bam_sorted.bam /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ_CONTIG_ASSEMBLER/ASSEMBLE_VDJ/fork0/chnk002-ucc6a7a72ce/files/contig_bam.bam

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10.437 seconds used processing barcodes, peak mem = 0.23
