[stdout]

starting vdj_asm_asm, mem = 0.01
available mem = 844.38 GB
/scratch/etanis/software/cellranger-6.1.2/lib/bin/cr_vdj martian assembly main /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GE...
n50_n50_rpu = 288

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1 = AAACCTGAGAGCTATA-1

using 307 reads

====================================================================================

graph has 68 edges initially, 2 edges after simplification

total ucounts = 2
nonsolo ucounts = 1[306]
surviving nonsolo ucounts = 1[306]
ids = [1]

====================================================================================

UMI info for barcode AAACCTGAGAGCTATA-1 contig 1 = TGGGATCAGT...
umi TTTTCAGTCT = 290 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=509]
0-27 ==> 0-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=2)
27-378 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=6)
377-415 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
415-509 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 46 reads
cdr3 = CLQHKSYPFTF at 354, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 289
start codons at 27, 33, 102, 238, 457
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.6 = AAACCTGAGCGTAGTG-1

using 117 reads

====================================================================================

graph has 26 edges initially, 2 edges after simplification

total ucounts = 4
nonsolo ucounts = 1[114]
surviving nonsolo ucounts = 1[114]
ids = [1]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.8 = AAACCTGAGCGTTTAC-1

using 139 reads

====================================================================================

graph has 44 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[139]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.9 = AAACCTGAGCTGAACG-1

using 1427 reads

====================================================================================

graph has 1935 edges initially, 22 edges after simplification

total ucounts = 642
nonsolo ucounts = 293[2^120, 3^73, 4^32, 5^26, 6^17, 7^8, 8^6, 10^2, 11^2, 12, 13^2, 15, 16, 20, 24]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.11 = AAACCTGAGGAGTTTA-1
... 25312 lines elided ...
47-400 ==> 0-353 on |323|IGLV1-44|L-REGION+V-REGION| [len=353] (mis=15)
397-435 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=3)
435-511 ==> 0-76 on |306|IGLC2|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CASWDDSLRGRVF at 368, score = 8 + 8
umis assigned: [410]
of which 1 are surviving nonsolos
reads assigned: 170
start codons at 47, 201, 351, 376, 381
confident = false

TIG 2[bases=515]
0-33 ==> 14-47 on |278|IGKV3-11|5'UTR| [len=47] (mis=0)
33-378 ==> 0-345 on |279|IGKV3-11|L-REGION+V-REGION| [len=345] (mis=0)
384-421 ==> 0-37 on |216|IGKJ4|J-REGION| [len=37] (mis=0)
421-515 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 34 reads
cdr3 = CQQRSNWPGALTF at 354, score = 9 + 9
umis assigned: [270]
of which 1 are surviving nonsolos
reads assigned: 253
start codons at 33, 238, 241, 463
confident = false

TIG 3[bases=509]
0-27 ==> 154-181 on |260|IGKV1D-8|5'UTR| [len=181] (mis=0)
27-380 ==> 0-353 on |261|IGKV1D-8|L-REGION+V-REGION| [len=353] (mis=3)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
415-509 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 45 reads
cdr3 = CQQYYSFPWTF at 354, score = 9 + 8
umis assigned: [427]
of which 1 are surviving nonsolos
reads assigned: 242
start codons at 27, 33, 89, 102, 165, 238, 457
confident = false

TIG 4[bases=515]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=1)
33-381 ==> 0-348 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=25)
382-421 ==> 0-39 on |214|IGKJ2|J-REGION| [len=39] (mis=4)
421-515 ==> 0-94 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 28 reads
cdr3 = CQQYGSSPMYTF at 357, score = 9 + 8
umis assigned: [243]
of which 1 are surviving nonsolos
reads assigned: 159
start codons at 33, 82, 241, 340, 381, 463
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1807 = AACTCCCGTCCATCCT-1

using 396 reads

====================================================================================

graph has 74 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 2[4, 389]
surviving nonsolo ucounts = 1[389]
ids = [4]

====================================================================================

UMI info for barcode AACTCCCGTCCATCCT-1 contig 1 = GGCTTTCTGA...
umi TTGCCCATGT = 386 reads: +448 validated

GOOD CONTIGS

TIG 1[bases=534]
15-392 ==> 0-377 on |190|IGHV4-39|L-REGION+V-REGION| [len=377] (mis=6)
412-463 ==> 0-51 on |57|IGHJ5|J-REGION| [len=51] (mis=2)
463-534 ==> 0-71 on |60|IGHM|C-REGION| [len=1358] (mis=0)
junction support: 1 umis using 22 reads
cdr3 = CARQADVLTGYYWFDPW at 381, score = 9 + 6
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 384
start codons at 15, 24, 36, 80, 397
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1816 = AACTCCCGTCTTTCAT-1

using 163 reads

====================================================================================

graph has 46 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 2[2, 154]
surviving nonsolo ucounts = 1[154]
ids = [3]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1827 = AACTCCCGTGGTACAG-1

using 8138 reads

====================================================================================

graph has 7482 edges initially, 126 edges after simplification

total ucounts = 1502
nonsolo ucounts = 1087[2^228, 3^131, 4^109, 5^115, 6^90, 7^75, 8^81, 9^62, 10^35, 11^33, 12^36, 13^26, 14^18, 15^9, 16^8, 17^10, 18^4, 19^4, 20^2, 21, 22^2,...
surviving nonsolo ucounts = 3[180, 333, 338]
ids = [260, 625, 45]

====================================================================================

UMI info for barcode AACTCCCGTGGTACAG-1 contig 1 = GTCAGTCCCA...
umi AAGTTCACGA = 338 reads: +394 validated
umi ATACTTCGGC = 182 reads: +394 validated
umi CGGGTCTTCG = 332 reads: +394 validated

GOOD CONTIGS

TIG 1[bases=553]
0-23 ==> 8-31 on |226|IGKV1-33|5'UTR| [len=31] (mis=0)
23-374 ==> 0-351 on |227|IGKV1-33|L-REGION+V-REGION| [len=351] (mis=0)
379-417 ==> 0-38 on |215|IGKJ3|J-REGION| [len=38] (mis=0)
417-553 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 3 umis using 148 reads
cdr3 = CQQYDNLPPLFTF at 350, score = 9 + 8
umis assigned: [45, 260, 625]
of which 3 are surviving nonsolos
reads assigned: 838
start codons at 23, 29, 85, 98, 237, 360, 459
confident = true
now this is a cell
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1833 = AACTCCCGTTAGAACA-1

using 126 reads

====================================================================================

graph has 48 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[3^2, 4, 112]
surviving nonsolo ucounts = 1[112]
ids = [4]

====================================================================================

UMI info for barcode AACTCCCGTTAGAACA-1 contig 1 = GAAGAGCTGC...
umi GAATGTAGTC = 102 reads: +382 validated

GOOD CONTIGS

TIG 1[bases=448]
0-33 ==> 19-52 on |282|IGKV3-20|5'UTR| [len=52] (mis=0)
33-370 ==> 0-337 on |283|IGKV3-20|L-REGION+V-REGION| [len=348] (mis=13)
377-415 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
415-448 ==> 0-33 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 15 reads
cdr3 = CQQYDRSWTF at 357, score = 9 + 8
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 102
start codons at 33, 367
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1838 = AACTCCCGTTCCCTTG-1

using 54 reads

====================================================================================

graph has 62 edges initially, 2 edges after simplification

total ucounts = 7
nonsolo ucounts = 4[2, 3, 6, 40]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1845 = AACTCCCGTTTGCATG-1

using 11 reads

====================================================================================

graph has 14 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[11]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1847 = AACTCCCTCAAACCGT-1

using 227 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 10
nonsolo ucounts = 7[2^3, 3, 4, 7, 204]
surviving nonsolo ucounts = 1[204]
ids = [6]

====================================================================================

UMI info for barcode AACTCCCTCAAACCGT-1 contig 1 = GGAATCAGTC...
umi GAGGCTCAAA = 205 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=550]
0-26 ==> 1-27 on |222|IGKV1-17|5'UTR| [len=27] (mis=0)
26-377 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
414-550 ==> 0-136 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 41 reads
cdr3 = CLQYSSSPWTF at 353, score = 9 + 8
umis assigned: [6]
of which 1 are surviving nonsolos
reads assigned: 198
start codons at 26, 32, 101, 237, 456
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1855 = AACTCCCTCACATGCA-1

using 135 reads

====================================================================================

graph has 78 edges initially, 4 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2, 3, 22, 104]
surviving nonsolo ucounts = 2[22, 104]
ids = [4, 7]

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1859 = AACTCCCTCACTTATC-1

using 22 reads

====================================================================================

graph has 12 edges initially, 2 edges after simplification

total ucounts = 1
nonsolo ucounts = 1[22]
surviving nonsolo ucounts = 0[]
ids = []

====================================================================================
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1861 = AACTCCCTCAGAAATG-1

using 162 reads

====================================================================================

graph has 88 edges initially, 2 edges after simplification

total ucounts = 12
nonsolo ucounts = 5[3^2, 4, 5, 140]
surviving nonsolo ucounts = 1[140]
ids = [11]

====================================================================================

UMI info for barcode AACTCCCTCAGAAATG-1 contig 1 = GGAGTCAGAC...
umi TTCACTTGAA = 140 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=449]
0-26 ==> 155-181 on |232|IGKV1-5|5'UTR| [len=181] (mis=0)
26-377 ==> 0-351 on |233|IGKV1-5|L-REGION+V-REGION| [len=351] (mis=2)
376-414 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=0)
414-449 ==> 0-35 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 20 reads
cdr3 = CQQYNSYSWTF at 353, score = 8 + 8
umis assigned: [11]
of which 1 are surviving nonsolos
reads assigned: 137
start codons at 26, 32, 88, 101, 333
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1862 = AACTCCCTCAGAGACG-1

using 310 reads

====================================================================================

graph has 134 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 4[2^2, 7, 295]
surviving nonsolo ucounts = 1[295]
ids = [7]

====================================================================================

UMI info for barcode AACTCCCTCAGAGACG-1 contig 1 = GGGGATGGCC...
umi TAATACCCTA = 282 reads: +391 validated

GOOD CONTIGS

TIG 1[bases=534]
4-360 ==> 0-356 on |321|IGLV1-40|L-REGION+V-REGION| [len=356] (mis=1)
357-395 ==> 0-38 on |313|IGLJ3|J-REGION| [len=38] (mis=0)
395-534 ==> 0-139 on |307|IGLC3|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 68 reads
cdr3 = CQSYDSSLSGWVF at 328, score = 8 + 8
umis assigned: [7]
of which 1 are surviving nonsolos
reads assigned: 277
start codons at 4, 158, 161, 212, 311, 338
confident = false
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1873 = AACTCCCTCATGCAAC-1

using 178 reads

====================================================================================

graph has 100 edges initially, 2 edges after simplification

total ucounts = 14
nonsolo ucounts = 4[3, 4^2, 157]
surviving nonsolo ucounts = 1[157]
ids = [10]

====================================================================================

REJECT CONTIGS

TIG 1[bases=505]
0-80 ==> 0-80 on |123|IGHV3-30|5'UTR| [len=80] (mis=1)
49-116 ==> 6507-6574 on segment before IGHV3OR16-9 exon 1 [len=6692] (mis=2)
80-431 ==> 0-351 on |124|IGHV3-30|L-REGION+V-REGION| [len=351] (mis=0)
436-467 ==> 0-31 on |13|IGHD2-15|D-REGION| [len=31] (mis=5)
462-505 ==> 0-43 on |55|IGHJ4|J-REGION| [len=48] (mis=3)
cdr3 = CAKVTGYCSGGSCPDLDYW at 422, score = 9 + 7
umis assigned: [10]
of which 1 are surviving nonsolos
reads assigned: 155
start codons at 80, 231, 236, 294, 297, 315, 383
confident = false
VJ delta = 6
not full
not full
NOT paired!

▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓▓

BARCODE 0.1882 = AACTCCCTCCCTTGCA-1

using 286 reads

====================================================================================

graph has 112 edges initially, 2 edges after simplification

total ucounts = 5
nonsolo ucounts = 3[2, 7, 275]
surviving nonsolo ucounts = 1[275]
ids = [3]

====================================================================================

UMI info for barcode AACTCCCTCCCTTGCA-1 contig 1 = GCTGGGGTCA...
umi TCATGCACCG = 273 reads: +385 validated

GOOD CONTIGS

TIG 1[bases=541]
0-41 ==> 121-162 on |342|IGLV2-23|5'UTR| [len=162] (mis=0)
41-381 ==> 0-340 on |343|IGLV2-23|L-REGION+V-REGION| [len=340] (mis=11)
388-426 ==> 0-38 on |309|IGLJ1|J-REGION| [len=38] (mis=1)
426-541 ==> 0-115 on |305|IGLC1|C-REGION| [len=317] (mis=0)
junction support: 1 umis using 38 reads
cdr3 = CCSKAGRSYVF at 365, score = 8 + 8
umis assigned: [3]
of which 1 are surviving nonsolos
reads assigned: 270
start codons at 41, 180, 249, 390
confident = false
NOT paired!

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BARCODE 0.1886 = AACTCCCTCCGCGCAA-1

using 234 reads

====================================================================================

graph has 108 edges initially, 2 edges after simplification

total ucounts = 8
nonsolo ucounts = 3[2, 3, 224]
surviving nonsolo ucounts = 1[224]
ids = [1]

====================================================================================

UMI info for barcode AACTCCCTCCGCGCAA-1 contig 1 = GGGGAGGAAT...
umi AGGCCCTGGT = 219 reads: +388 validated

GOOD CONTIGS

TIG 1[bases=509]
31-382 ==> 0-351 on |223|IGKV1-17|L-REGION+V-REGION| [len=351] (mis=19)
381-419 ==> 0-38 on |213|IGKJ1|J-REGION| [len=38] (mis=1)
419-509 ==> 0-90 on |212|IGKC|C-REGION| [len=320] (mis=0)
junction support: 1 umis using 32 reads
cdr3 = CLQYSSSPWTF at 358, score = 9 + 8
umis assigned: [1]
of which 1 are surviving nonsolos
reads assigned: 214
start codons at 31, 37, 106, 242, 461
confident = false
NOT paired!

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BARCODE 0.1896 = AACTCCCTCGCGTAGC-1

using 85 reads

====================================================================================

graph has 42 edges initially, 2 edges after simplification

total ucounts = 9
nonsolo ucounts = 4[2^3, 74]
surviving nonsolo ucounts = 1[74]
ids = [4]

====================================================================================

REJECT CONTIGS

TIG 1[bases=393]
0-30 ==> 16-46 on |389|IGLV8-61|5'UTR| [len=46] (mis=0)
30-393 ==> 0-363 on |390|IGLV8-61|L-REGION+V-REGION| [len=368] (mis=0)
umis assigned: [4]
of which 1 are surviving nonsolos
reads assigned: 70
start codons at 30, 45, 54, 57, 82, 349, 352, 381
confident = false
did not find CDR3
NOT paired!
sorting bam, mem = 0.14
running samtools sort -l 8G -o /scratch/etanis/ST-118/multi/dimitri-multi/SC_MULTI_CS/SC_MULTI_CORE/MULTI_GEM_WELL_PROCESSOR/VDJ_B_GEM_WELL_PROCESSOR/SC_VDJ...

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177.811 seconds used processing barcodes, peak mem = 0.34
