Parameter Value Version 1.5.0.0 Fixed modifications Carbamidomethyl (C) Decoy mode revert Special AAs KR Include contaminants True MS/MS tol. (FTMS) 20 ppm Top MS/MS peaks per 100 Da. (FTMS) 12 MS/MS deisotoping (FTMS) True MS/MS tol. (ITMS) 0.5 Da Top MS/MS peaks per 100 Da. (ITMS) 8 MS/MS deisotoping (ITMS) False MS/MS tol. (TOF) 40 ppm Top MS/MS peaks per 100 Da. (TOF) 10 MS/MS deisotoping (TOF) True MS/MS tol. (Unknown) 0.5 Da Top MS/MS peaks per 100 Da. (Unknown) 8 MS/MS deisotoping (Unknown) False PSM FDR 0.01 Protein FDR 0.01 Site FDR 0.01 Use Normalized Ratios For Occupancy True Min. peptide Length 7 Min. score for unmodified peptides 0 Min. score for modified peptides 40 Min. delta score for unmodified peptides 0 Min. delta score for modified peptides 9 Min. unique peptides 0 Min. razor peptides 1 Min. peptides 1 Use only unmodified peptides and True Modifications included in protein quantification Acetyl (Protein N-term);Oxidation (M) Peptides used for protein quantification Razor Discard unmodified counterpart peptides True Min. ratio count 2 Re-quantify False Use delta score False iBAQ True iBAQ log fit True Match between runs True Matching time window [min] 0.5 Alignment time window [min] 20 Find dependent peptides False Fasta file C:\Users\MS_Analysis\Desktop\MaxQuant_1.3.5.7_NQ\HUMAN.fasta First search fasta file C:\Users\MS_Analysis\Desktop\MaxQuant_1.3.5.7_NQ\human.first.search.fasta Labeled amino acid filtering True Site tables Oxidation (M)Sites.txt Decoy mode revert Special AAs KR Include contaminants True RT shift False Advanced ratios True AIF correlation 0.47 First pass AIF correlation 0.8 AIF topx 20 AIF min mass 0 AIF SIL weight 4 AIF ISO weight 2 AIF iterative True AIF threshold FDR 0.01